The monsters shown in the movie are all references to original monsters in movies. Ginormica is a reference to Attack of the 50 Foot Woman (1958), B.O.B. is a reference to The Blob (1958), The Missing Link is a reference to Creature from the Black Lagoon (1954), Dr. Cockroach Ph.D. is a reference to The Fly (1958), and Insectosaurus is a reference to Mothra from Mosura (1961).

For the scenes of Ginormica wandering the streets of San Francisco, a replica of the set was built, and supervising animator Line Korsgaard Andersen was videotaped walking around it as reference for the animators.

When Gallaxhar is talking to Earth about what he is about to do, when he is talking in Tokyo, Japan, on the top left corner, a scene from Madagascar (2005) can be seen of Marty talking to Alex.

The first computer-animated movie directly produced in a stereoscopic 3-D format, instead of being converted into 3-D after completion.

If this movie had been rendered on one computer, it would have taken 4,071 years to render.