The last of the X-Men, an aging James "Logan" Howitt (Hugh Jackman) and his dying longtime friend and mentor Charles Xavier (Patrick Stewart) become the protectors of Laura Kinney (Dafne Keen), an 11-year-old mutant girl from the corrupt Alkali-Transigen corporation. When they discover the corporation is using mutant DNA samples to create and control mutants and use them as weapons and that the corporation also created Laura from Logan's mutant DNA, they set out to take Laura to Eden, a refuge for mutants, and Logan makes his final stand as he fights Alkali-Transigen that are after Laura and other mutant children that they created.

While this film's story pulls inspiration and story ideas from several Wolverine comic book stories, the film itself is not an adaptation of any particular previously-published story. The story most noted for inspiring this film is Mark Millar's 2008 "Old Man Logan" mini-series. However, the final film bears no resemblance to that comic book except for minor thematic parallels.

No, Patrick Stewart did reprise the role in Doctor Strange and The Multiverse of Madness for a minor role as a Professor X in a different dimension. Hugh Jackman reprises his role of Wolverine in Deadpool & Wolverine.