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Model Question Paper B.Tech. Industrial Biotechnology (V Semester) Ib038 - Metabolic Engineering

The document is a model question paper for an exam on metabolic engineering. It contains two parts: Part A contains 10 short answer questions testing fundamental concepts in metabolic engineering like flux control coefficients, reverse isotope trapping techniques, energy charge, regulation of key metabolic enzymes, effects of glucagon, glucose metabolism pathways, analog resistant mutants, inverse metabolic engineering, and metabolic control. Part B contains 4 long answer questions, one of which is compulsory, related to criteria for choosing organism for metabolite overproduction, techniques for strain improvement like isolation of auxotrophs and analog resistant mutants, use of rational design and evolutionary engineering in metabolic engineering, selection of mutants for primary/secondary metabolite overproduction, and directing

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373 views2 pages

Model Question Paper B.Tech. Industrial Biotechnology (V Semester) Ib038 - Metabolic Engineering

The document is a model question paper for an exam on metabolic engineering. It contains two parts: Part A contains 10 short answer questions testing fundamental concepts in metabolic engineering like flux control coefficients, reverse isotope trapping techniques, energy charge, regulation of key metabolic enzymes, effects of glucagon, glucose metabolism pathways, analog resistant mutants, inverse metabolic engineering, and metabolic control. Part B contains 4 long answer questions, one of which is compulsory, related to criteria for choosing organism for metabolite overproduction, techniques for strain improvement like isolation of auxotrophs and analog resistant mutants, use of rational design and evolutionary engineering in metabolic engineering, selection of mutants for primary/secondary metabolite overproduction, and directing

Uploaded by

devikamurugan124206
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© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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MODEL QUESTION PAPER B.Tech. Industrial Biotechnology (V Semester) IB038 - METABOLIC ENGINEERING Max.

Marks: 100 PART A (10 X 2 = 20 Marks) Answer ALL the Questions 1. What is MCA? Define flux control coefficient? 2. What is reverse isotope trapping and overloading technique? What is their use? 3. What is energy charge? Why is it important? 4. How are isocitrate dehydragenase and citrate synthase regulated? 5. What are the metabolic effects of glucagon on glucose metabolism? 6. With the help of only a diagram illustrate the various pathways for glucose 6phosphate. 7. What are analog resistant mutants and revertants? 8. What is inverse metabolic engineering? 9. Define network rigidity, flexible and rigid modes. 10. What are the different means by which metabolic control is executed? PART B (4 X 20 = 80 Marks) 1) Answer any FOUR Questions. 2) Question No. 11 is Compulsory 11. What are the criteria to be employed in the choice of an organism for metabolite overproduction? List the sequence of steps and the strategy involved in (a) the isolation of a mutant of C.glutamicum overproducing lysine that does not recognize inhibitors or repressors. (b) The isolation of a mutant of C.glutamicum overproducing glutamic acid. 12. Describe the different techniques employed in the isolation of auxotrophs and ARM. Diagram a flow chart for the strain improvement programme for secondary metabolic production. Time: 3 hours

OR What are the potentials and perils of rational design in the metabolic engineering cycle? How does evolutionary engineering fill gaps in the rational design cycle? 13. Give a blueprint for the selection of induced mutants synthesizing improved levels of primary metabolites with suitable examples of pathways. OR Give a concise review of your seminar paper. 14. Give the central metabolic pathway in E.coli. Using techniques of genetic engineering and the strategies of metabolic engineering, describe how you will direct the flux of this pathway to overproduce propionic acid. How may one use evolutionary engineering towards this end? 15. Outline a strategy for the selection of induced mutants synthesizing improved levels of primary metabolites. How is it different from the methods involving secondary metabolic production?

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