Buck Scientific, Inc.

Seminar in ANALYTICAL INSTRUMENTATION for Spectroscopy & Chromatography: UV-Vis Spectrophotometry

Ultra-Violet/Visible SPECTROSCOPY (UV-Vis)

Theory & Principle of OPERATION

A TYPE of VIBRATIONAL Spectroscopy, similar to Infra-Red. A Sample in a Cuvette is exposed to Light Energy between 190nm. (in the UV) and 1000nm. (in the VIS). Certain parts of an Organic Molecule will ABSORB some of this energy to create PEAKS on a SPECTRUM for Quantitative (primarily) and Qualitative Analysis

BASIC Spectrophotometer Design

Like AAS instrumentation, the ORIGINAL UV-Vis Specs were made as DOUBLE-BEAM units to correct for Noise, Drift and other Instabilities. Over 20 years ago, the well-known leaders in the Analytical Instrument markets; Beckman & PerkinElmer; began to focus on a line of Stable-Beam SINGLE-BEAM Instruments.

The BUCK/Cecil series employs this optimum design in our instruments with unique Internal software

Basic SPECTROMETER Design

ALL Analytical Spectrometers have several BASIC design components in common:

Light Source Sample Chamber Optical System Detector Output

Basic SPECTROMETER Design

ALL Analytical Spectrometers have several BASIC design components in common:
LIGHT SOURCE: External D2 & W Lamps to prevent
thermal instability within instrument (no fan)

SAMPLE Chamber: Standard & Specialty Holders OPTICS: High-energy Ebert Monochromator DETECTOR: Choice of low-Noise Photo-Diode or high-Sensitivity Photo-Multiplier Tube OUTPUT: Graphics On-Screen Display, Analog, Printer
and Serial RS-232 P-C capabilities

UV/Vis Important Features

Scan Speed Resolution Software Features Ease of Operation Data Storage Customized Calculations Support & Service

BUCK/Cecil Instruments
The COMPACT, Stable-Beam construction of the Single-Beam series uses the high-quality Cecil base instrument and high-performance components from BUCK Scientific

BUCK/Cecil Instruments
Best optical design and highest quality components resulting in superior specifications:
Stray Light Noise Stability Baseline Flatness Accuracy (wavelength & photometric / ABS) Reproducibility (wavelength & photometric / ABS)

BUCK/Cecil Series
Series 2000
Single Beam, Scanning, 4nm bandpass Optional built-in printer & P-C Interface Many software options Most POPULAR Industrial system Applications: Bio-Quest, Aqua-Quest, FoodQuest series with Prep-Programmed Operations

BUCK/Cecil Series
Series 3000
Single Beam, Scanning, 1.8nm bandpass Optional built-in printer & P-C interface Many software features (Kinetics, Bio-Assay) Reflectance option: 3055 w/ Integrating Sphere

Specifically designed to meet ALL of the required Specifications for USP / BP Pharmaceutical Assays

BUCK/Cecil Series
Series 7000/7000+/9000
Double-Beam, Scanning, some Models with
variable 0.5 / 1.0 / 1.8-2.0 / 4.0nm bandpass

Standard Printer & P-C interface, Data-Stream Many software features (Kinetics, Bio-Assay) Reflectance / Fluorescence / Turbidity options

Ideal for the stringent requirements of Academic and Pharmaceutical Research

Sample Handling Options

Cuvette - standard cell holder
Quartz for UV range Glass & Plastic for Vis range 1-100mm Pathlengths

Flow Cell - standard or micro flow (50L)

Uses Sipette Pump Optional Automatic Cell Changer

Sample Handling Options

Multi-Cell Changer
4, 6, or 8 Positions Manual, Automatic, Programmable

Thermostatted Cell Holder

Water-jacketted or Electronic Peltier system

Batch Sampler - 40 Positions Carousel Specular & Integrating Sphere / Diffuse Reflectance

Sample Handling Options

Multiple position CELL CHANGERS, MICROSIPPERS and Sequential Access BATCH SAMPLERS Allow the Chemist complete FLEXIBILITY for MAXIMUM Sample Throughput & Volume on ANY of the BUCK/Cecil UV-Vis Spectrophotometers

Software Features
A convenient INTERNAL Ink-Printer, and several P-C Software Programs provide many Data Processing and Manipulation options for Qualitative & Quantitative Work Economical UV-Scan and sophisticated GRAMS/UV & Data-Stream are available

Software Features
The INTERNAL Software built-in to the BUCK/Cecil UV-Vis Spectrophotometers is capable of performing General Analytical, Environmental, Biotechnology and Clinical Assay WITHOUT the need for a Computer:
Christian-Warburg & Protein / DNA Measurements Colorimetric Anion Calibrations stored in the instrument Wavelength Subtraction, Spectral Overlay & Smoothing, Derivatization and auto Peak-Pick on all systems Multi-Component Analysis, with 1o / 2o and 3o Polynomial Calibrations with up to 10 Standards with Statistics

APPLICATIONS
Primarily used for QUANTITATIVE Analysis of Known Compounds, UVVis is one of the most popular techniques in the Pharmaceutical, Foods and Paints Industries, as well as Water Laboratories

APPNOTE - Inorganic
SAMPLE: River Water for PHOSPHATE PREPARATION: Digest w/ 10% HCl, add NH4MoO4 & Hydrazine reagent, boil till Blue color develops; measure at 810nm. ANALYSIS: CE-2041 or VIS-100 w/ USEPA 2-pt Calibration

RESULTS: mg/L
or PPM in Sample

APPNOTE - Inorganic
DATE 3-10-00 3-24-00 4-08-00 4-22-00 5-10-00 Total-PO4 69 PPM 77 PPM 94 PPM 118 PPM 85 PPM ortho-PO4 51 PPM 50 PPM 83 PPM 72 PPM 78 PPM

ANION assays by Colorimetry are the ACCEPTED Standard World-Wide & much better (and less expensive) than Ion-Chromatography

APPNOTE - Cosmetic
SAMPLE: Color-Dyes for Hair Shampoos PREPARATION: Make 1:5 and 1:200 dilution of Dyes to compare to Samples ANALYSIS: CE-2041 or -3041 Scanning Spectrophotometer, 1cm. Glass Cuvette

RESULT: % Red /
Green / Blue based on Color Standards

APPNOTE - Nutritional
SAMPLE: Cereals and Grains (preserved) PREPARATION: Hydrolyze 2 grams w/ 5% HCl, extract w/ Hexane for 1:10 dilution ANALYSIS: CE-2041 or CE-3041 Scanning Spectrophotometer, scan range 200-450nm. For BHT, BHA and TBHQ Anti-Oxidants. Sample solutions run in 10mm. Quartz Cells

RESULT: Parts-Per-Million TOTAL AntiOxidants based on USFDA Standard Procedure

APPNOTE - Nutritional
Sample
[Wavelength

BHA
410nm.

BHT
360nm.

TBHQ
285nm.]

Frosted Flakes Raisin Bran Rice Krispies

35 PPM 16 PPM 23 PPM

27 PPM 41 PPM 11 PPM

11 PPM < 2 PPM 4.5 PPM

Scanning SPECTROPHOTOMETRY is an ideal method to evaluate YOUR OWN products, or material from the Competition

Other SPECTRO Analyzers from BUCK

BUCK Scientific also offers a series of inexpensive, high-performing and reliable Analytical Spectroscopy equipment for special Applications, Educational and Dedicated Testing procedures

FLUORESCENCE SPECTROSCOPY (FLUOR)

Theory & Principle of OPERATION

FLUORESCENCE is a form of Molecular EMISSION Spectroscopy; where a Sample in a special Cuvette is exposed to invisible, highEnergy UV radiation, and certain parts of the Organic Molecule will give off or EMIT lowerEnergy VISIBLE Light radiation. Very specific & selective test for Drugs & Vitamins, Alkaloids & Toxins

BUCK Filter Fluorometer

The advantage of HIGH Sensitivity for most compatible materials makes Fluorescence a valuable analytical tool for Forensic & Drug applications
Intense BLUE Fluorescence of QUININE in a Drug Formulation

BUCK Filter Fluorometer

The availability of a wide assortment of Excitation and Emission Filters makes this portable FL-380 instrument very flexibility for a very low cost ideal for Academic Teaching & Industrial QA/QC work

Visible COLORIMETRY (COLOR)

Theory & Principle of OPERATION

This is for use in Academic Teaching classes and for dedicated COLORIMETRIC Analyses for materials such as ANIONS (SO4, NO2, NO3, PO4, etc) and Visible Colors of compound such as Pigments, Chlorophyll and Dyes

Covers the range from 320 - 950nm.

BUCK Colorimeter
The VIS-100 is a small, durable, selfcalibrating unit that provides MEDIUM resolution @ 8nm. Bandpass, automatic 1-point Calibration & Data Outputs for a very low price