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The document describes coating wells of a plate with various proteins including Protein G, TF9, mouse IgG, and Fibronectin alone or in combination with 1% BSA. Additional steps include washing the wells with ethanol, PBS, coating with Protein G or 5% BSA, adding cells to the wells, washing with PBS, and visualizing under a microscope.

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Anchalia Chandrakumaran
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© Attribution Non-Commercial (BY-NC)
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56 views1 page

Diagram

The document describes coating wells of a plate with various proteins including Protein G, TF9, mouse IgG, and Fibronectin alone or in combination with 1% BSA. Additional steps include washing the wells with ethanol, PBS, coating with Protein G or 5% BSA, adding cells to the wells, washing with PBS, and visualizing under a microscope.

Uploaded by

Anchalia Chandrakumaran
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Protein G (50 ug/mL ), 1% BSA

Protein G (50 ug/mL ), TF9 (50 ug/mL), 1% BSA

Protein G (50 ug/mL ), mouseIgG (50 ug/mL), 1% BSA

Protein G (50 ug/mL ), Fibronectin (10 ug/mL), 1% BSA

1% BSA

Washed with ethanol

Washed 3 times with 1xPBS

Coated with 50 ug/mL Protein G

Washed 3 times with 1xPBS

Washed 3 times with 1xPBS

Coated with 5%BSA

Washed 3 times with 1xPBS

Coated three wells with TF9 (50 ug/mL) , mouseIgG (50 ug/mL), or Fibronectin (10 ug/mL)

Added cells (1x10^6 cells/mL) into the wells

Washed 3 times with 1xPBS

Visualized under microscope (10x objective)

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