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Order of Draw

This document provides information on blood collection tubes including their color, additive, and common tests. It lists 16 different tubes in order of draw: 1. Clear tube is used as a discard tube. 2. Blood culture bottle must be drawn first if a culture is ordered with other bloodwork. 3. Yellow tube is used for mycobacteria blood cultures. 4. Royal blue tube with red label is used for serum trace element tests. 5. Red glass tube is used for serum tests that cannot be in SST tubes.
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0% found this document useful (0 votes)
676 views2 pages

Order of Draw

This document provides information on blood collection tubes including their color, additive, and common tests. It lists 16 different tubes in order of draw: 1. Clear tube is used as a discard tube. 2. Blood culture bottle must be drawn first if a culture is ordered with other bloodwork. 3. Yellow tube is used for mycobacteria blood cultures. 4. Royal blue tube with red label is used for serum trace element tests. 5. Red glass tube is used for serum tests that cannot be in SST tubes.
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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ORDER OF DRAW

ORDER
OF
DRAW

COLOR OF STOPPER

INVERTED

ADDITIVE

Clear

Not required

No additive

Blood culture bottle

Invert gently to
mix

Bacterial growth
medium and
activated charcoal

Yellow with clear label

8 to 10 times

Sodium polyanethol
sulfonate

Not required

Royal blue (with red


band on the label)
Red glass

Glass tube with no


additive
Glass tube with no
additive

Light blue

3 to 4 times

3.2% buffered
Sodium citrate
anticoagulant

Black glass

3 to 4 times

Red

5 times

3.2% Sodium citrate


anticoagulant
Clot activator and no
coagulant

Gold

5 times

Gel separator and


clot activator

10

Dark green

8 to 10 times

Sodium heparin
anticoagulant

Light green

8 to 10 times

Lithium heparin
anticoagulant and

7
8

11

Not required

COMMON TESTS

Tube used ONLY as


discard tube.
When a culture is
ordered along with any
other blood work, the
Blood Culture MUST be
drawn first.
Tube used for
Mycobacteria (ABF)
blood culture.
Tube used for serum
trace element test.
Tube used for serum
tests, which cannot be
collected in SST tubes.
Note: red plastic tubes
are preferable for lab
tests.
Tube used mainly for
PT (INR), PTT, and
other coagulation
studies.
Tube used for ESR only.
Tube used for serum
tests, which cannot be
collected in SST tubes.
Usually referred to as
SST (serum
separator tube). After
centrifugation, the Gel
forms a barrier
between the clot and
the serum.
Tube used mainly for
Amino Acids and
Cytogenetic Tests.
Usually referred to as
PST (Plasma

gel separator

12

Royal blue (with blue


band on the label)
Royal blue (with
lavender band on the
label)
Lavender

8 to 10 times

8 to 10 times

K2EDTA
anticoagulant

15

Yellow with yellow


banded white label

8 to 10 times

Acid citrate dextrose


solution A (ACDA)

16

Grey

8 to 10 times

Sodium fluoride and


potassium oxalate
anticoagulant

13

14

8 to 10 times

K2EDTA
anticoagulant
Na2EDTA
anticoagulant

separator tube). After


centrifugation, the gel
forms a barrier
between the blood
cells and the plasma.
Tube used mainly for
Chemistry tests.
Tube used for trace
elements.
Tube used for whole
blood trace element
trace.
Tube used mainly for
CBC, pre-transfusion
testing, Hemoglobin
A1C and anti-rejection
drugs.
Tube used for Tissue
Typing and some Flow
Cytometry testing
Tube used for lactate.

By: Jayson C. Ladio

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