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Catalase Enzyme Lab Report

This experiment tested the effect of different concentrations of potato catalase on its catalytic activity. Potato catalase was added to hydrogen peroxide solution at concentrations of 0%, 20%, 40%, 60%, 80%, and 100%. The amount of foam produced, indicating oxygen gas production, was measured for each concentration. Both the bench and class averages showed that as the concentration of catalase increased, the volume of foam also increased, supporting the hypothesis. At 100% catalase concentration, the average foam volume was over 10mL, demonstrating that higher enzyme concentrations lead to greater catalytic activity.

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296 views

Catalase Enzyme Lab Report

This experiment tested the effect of different concentrations of potato catalase on its catalytic activity. Potato catalase was added to hydrogen peroxide solution at concentrations of 0%, 20%, 40%, 60%, 80%, and 100%. The amount of foam produced, indicating oxygen gas production, was measured for each concentration. Both the bench and class averages showed that as the concentration of catalase increased, the volume of foam also increased, supporting the hypothesis. At 100% catalase concentration, the average foam volume was over 10mL, demonstrating that higher enzyme concentrations lead to greater catalytic activity.

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Sara
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Catalytic Activity of Enzymes

Introduction:
The purpose of this lab is to find out which concentration of catalase will the enzyme be
most active. Catalase has a main goal of breaking down hydrogen peroxide, which is its
substrate. At different concentration levels, H2O2 has different effects. At high levels, it can kill
a cell without catalase, which removes the harmful products and leaves healthy, harmless
molecules. Catalases are found in all cells grown in oxygen (1). For this experiment, a potato
will be used, which cells were grown in oxygen. To release catalase, grind up potatoes in water
in a blender. To separate cell debris from liquid and potato skins, the potato should be filtered
through cheese cloth. The liquid will contain the catalase.
Enzymes are proteins responsible for most of the chemical activities of living organisms.
They act as catalysts, which are substances that speed up chemical reactions without being
destroyed or completely changed during the process. Enzymes are extremely efficient and may
be used over and over again. One enzyme may catalyze thousands of reactions every second.
However, enzymes are very specific. Enzymes are highly specific both in the reactions that they
catalyze and in their choice of reactants, which are called substrates. An enzyme usually
catalyzes a single chemical reaction or a set of closely related reactions (3).
Catalase activity will be measured by adding potato cell extract to 3% hydrogen peroxide
solution in a test tube. Bubbles of O2 gas creates foam in the test tube when the catalysis occurs,
and oxygen is produced. The formation of bubbles, or oxygen, indicates a positive result. It is
hypothesized that the amount of foam produced by the potato cell extract will increase as the
enzyme concentration increases. The amount of foam is dependent upon the concentration of
both substrate hydrogen and catalase enzyme (2).

Materials and Methods:


In this experiment, Team A will test 0%, 20%, 40%, enzyme concentration in volume of
enzyme 0mL, 4mL, and 8mL, and volume of buffer at 20mL, 116mL, and 12mL-respectively.
Team B will test 60%, 80%, and 100% enzyme concentration in 12mL, 16mL, and 20mL
volume, and 8mL, 4mL, and 0mL of buffer-respectively. Label test tubes C, 1, 2, 3, 4, 5
respectively. Teams will add 2mL of 3% hydrogen peroxide to test tubes. Add 2mL of each of
the various dilution of catalase using a transfer pipet to corresponding test tubes containing
hydrogen peroxide. Wait 5 minutes for reactions. Measure bottom of test tube top to of foam in
mL, and record.

Results:
Table 1: Bench average of foam in milliliters.
Group

FOAM VOLUME IN
CONTROMILLILITERS
20%
40%
60%

80%

100%

GROUP 1

L
0.0mL

1.0mL

2.6mL

4.5mL

7.5mL

10.5mL

GROUP 2

0.0mL

0.5mL

2.0mL

5.0mL

8.0mL

10.0mL

BENCH

0.0mL

0.75mL

2.3mL

4.75mL

7.75mL

10.25mL

AVERAGE

Table 2: Class average of foam in milliliters.


Group
CONTRO
Bench 1
Bench 2
Bench 3
Class

L
0.0mL
0.0mL
0.0mL
0.0mL

FOAM VOLUME IN
20%
40%
60%
MILLILITERS
1.0mL
1.25mL
0.75mL
1.0mL

3.25mL
3.0mL
2.3mL
2.85mL

5.85mL
4.625mL
4.75mL
5.075mL

80%

100%

8.5mL
7.125mL
7.75mL
7.7917

11.5mL
9.875mL
10.25mL
10.542mL

Average
Upon adding potato catalase to 3% hydrogen peroxide buffer, the amount of foam
consistently increased. At 0% concentration, the amount of foam was equal to 0mL for both
bench and class averages. At 20%, foam volume was equal to 0.75mL for bench average and 1.0
for class average. At 40% concentration, the bench average foam volume was 2.3mL, and 2.85
for the class. At 60% concentration, foam volume was 4.75mL for both bench and class average.
At 80% concentration, foam volume was 7.75mL for bench average, and 7.7917mL for class
average. At 100% catalase concentration, foam volume was 10.25mL for class average, and
10.542mL for class average. Average volumes for bench and the class were consistently similar,
both rising as the potato catalase concentration level increased.

Effect of Catalase Concentration(%) on Catalase Activity Represented as Foam (mL)

Bench Average

Class Average

Figure 1: The bench and class averages were found to create a graph that directly represents each
value. At 100% catalase concentration,
Discussion:
The hypothesis that the amount of foam produced by the potato cell extract will increase
as the enzyme concentration increases was supported. Throughout the experiment, amount of

foam consistently increased. The final product, at 100% concentration, shows the foam activity
increased to 10.542 mL, as opposed to the beginning constant of 0% concentration with 0mL of
foam, or even the 20% concentrations product of and average of 1.0mL of foam activity.
Three-percent solution of hydrogen peroxide is used as a wound antiseptic. The
peroxides greatest and most important activity is towards Gram-positive bacteria, but the
presence of catalase in these bacteria makes dilutions below three percent less effective. Here,
what was tested in the lab has been made clear. The less concentration of hydrogen peroxide, the
less effective it will be.
References:
1. Catalytic Activity of Enzymes. Biology 1 Fall 2015 Lab Protocol Biol 111L. 29-32.
2. Cooper, Geoffrey M. "The Catalytic Activity of Enzymes." The Central Role of
Enzymes as Biological Catalysts. U.S. National Library of Medicine, 2000. Web. 07
Oct. 2015.
3. Morris, James. Biology How Life Works. Edition 13. W. H. Freeman. 24 July 2015. 55-5-6.

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