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Histologic Techniques PDF

This document outlines the 12 basic steps of histologic techniques: 1) Numbering, 2) Fixation, 3) Dehydration, 4) Clearing, 5) Infiltration, 6) Embedding, 7) Blocking, 8) Trimming, 9) Sectioning, 10) Staining, 11) Mounting, and 12) Labeling. It provides a brief description of each step, including fixing tissues to prevent decomposition, removing water through dehydration with alcohol, clearing with xylene to remove alcohol, infiltrating with paraffin wax, sectioning very thin tissues, staining typically with hematoxylin and eosin, and mounting stained slides with coverslips. The overall goal is to properly

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802 views18 pages

Histologic Techniques PDF

This document outlines the 12 basic steps of histologic techniques: 1) Numbering, 2) Fixation, 3) Dehydration, 4) Clearing, 5) Infiltration, 6) Embedding, 7) Blocking, 8) Trimming, 9) Sectioning, 10) Staining, 11) Mounting, and 12) Labeling. It provides a brief description of each step, including fixing tissues to prevent decomposition, removing water through dehydration with alcohol, clearing with xylene to remove alcohol, infiltrating with paraffin wax, sectioning very thin tissues, staining typically with hematoxylin and eosin, and mounting stained slides with coverslips. The overall goal is to properly

Uploaded by

Eya
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Basic

Histologic
Techniques
Alvin Rey Flores, RMT, MT(ASCPi),MPH

12 Steps of Histologic
Techniques
12 Steps
1. Numbering
2. FixaGon


3. DehydraGon
4. Clearing
5. InltraGon
6. Embedding

7. Blocking
8. Trimming
9. SecGoning
10. Staining
11. MounGng
12. Labeling

Numbering
First and Most Important Step
Logging in a Log book the details of the Gssue

Receiving of Specimens
Tissue Specimens should be properly labeled and with a
corresponding request
Tissues will be described by the pathologist (Gross
DescripGon)
Tissues will be cut by the pathologist

Fixation
Tissues are immersed in a xaGve to prevent decomposiGon,
and to preserve the structure

Dehydration
Removal of Excess Water Using Ascending Grades of Alcohol

Clearing (Dealcoholization)
Removal of excess alcohol; makes Gssues clear or transparent;
prepares Gssue for paran impregnaGon

InGiltration
Tissues caviGes are saturated with paran wax

Embedding
Impregnated Tissue is Oriented and Embedded in Paran
Block to form a Tissue Block

Blocking and Trimming


SeparaGon of embedded Gssues into Blocks
Removal of Excess Paran Wax using Knives

Sectioning
Very Thin SecGons of Tissue are Cut using a Microtome

Staining
Using H and E (RouGne Stain)

Mounting
Stained Tissue SecGons on a slide are added with mounGng
medium and are Covered with a Coverslip.

Labelling

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