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Unit 7 Enzyme Controlled Reactions Virtual Lab

The document describes an enzyme-controlled reaction virtual lab that tested the relationship between substrate concentration and initial reaction rate of lactase enzyme at different pH levels. The results show a linear relationship between substrate concentration and reaction rate up to a maximum initial reaction rate of 350 x 106 molecules formed per minute at pH 7 and a substrate concentration of 4.0g or higher. The optimum pH level for the lactase-catalyzed reaction is pH 7. Manufacturers of lactase supplements need to ensure the enzyme maintains a neutral pH as it travels through the digestive system.

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592 views2 pages

Unit 7 Enzyme Controlled Reactions Virtual Lab

The document describes an enzyme-controlled reaction virtual lab that tested the relationship between substrate concentration and initial reaction rate of lactase enzyme at different pH levels. The results show a linear relationship between substrate concentration and reaction rate up to a maximum initial reaction rate of 350 x 106 molecules formed per minute at pH 7 and a substrate concentration of 4.0g or higher. The optimum pH level for the lactase-catalyzed reaction is pH 7. Manufacturers of lactase supplements need to ensure the enzyme maintains a neutral pH as it travels through the digestive system.

Uploaded by

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© © All Rights Reserved
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Unit 7 Enzyme Controlled Reactions Virtual Lab

Amount of PH 3 PH 5 PH 7 PH 9 PH 11
substrate
0.5 g 19 39 72 45 24
1.0 g 39 81 145 91 49
2.0 g 82 168 300 189 103
4.0 g 96 198 350 223 121
8.0 g 96 198 350 223 121

Journal questions
1. Describe the relationship between substrate concentration and the initial
reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship?
What happens to the initial reaction rate as substrate concentration
increases?

As more substance is added from the enzymes, more products are being
formed. This is a linear relationship

2. What is the maximum initial reaction rate for the lactase enzyme at pH 7?
350 x 106 molecules formed per minute. It began at 4.0g and also appeared
in 8.0g

3. Explain why the maximum initial reaction rate cannot be reached at low
lactose concentrations.

The maximum initial reaction rate cannot be reached at low lactose


concentrations because the substrate will run out and there wouldn't be
enough enzymes to react on.

4. What does your data indicate about the optimum pH level for this lactase-
catalyzed reaction?

The optimum level for this lactase-catalyzed reaction is trial 5.

5. Enzymes function most efficiently at the temperature of a typical cell,


which is 37 degrees Celsius. Increases or decreases in temperature can
significantly lower the reaction rate. What does this suggest about the
importance of temperature-regulating mechanisms in organisms? Explain.
This suggest the importance of temperature as enzymes function more
efficiently when they are under the right temperature.

6. People with a lactose intolerance are able to take products such as Lactaid
that contain the lactase enzyme with their meals. These products can be
taken in pill form. Considering the fact that the pill form of the enzyme would
have to travel through the person's stomach, what special consideration
would the producer of this product need to be concerned about?

The main concern is whether the person consuming it is taking it in properly


or might not digest properly. For example, the lactase pH should stay neutral,
it shouldn't be too acidic or too basic because the person will not receive
enzymes as wished.

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