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LabBench Activity
BioCoach Lab Quiz I
LabBench Use the following information to answer questions 14.

In a molecular biology laboratory, a student obtained competent E. coli cells and used a
Glossary
common transformation procedure to induce the uptake of plasmid DNA with a gene for
resistance to the antibiotic kanamycin. The results below were obtained.
Molecular Biology

Introduction

6-I BACTERIAL
TRANSFORMATION

Key Concepts I
Concept 1: Bacterial
Colonies
Concept 2: E.coli Bacteria
Concept 3: Plasmids
Concept 4: Competent 1. On which petri dish do only transformed cells grow?
Cells
a. Plate I
Design of the Experiment I b. Plate II
Exercise 1: Transformation
c. Plate III
Procedure
d. Plate IV
Closer Look: Step 1
Closer Look: Step 2 Correct! The correct answer is b, Plate II.
Closer Look: Step 3
Closer Look: Step 4 2. Which of the plates is used as a control to show that nontransformed E. coli will not grow
Closer Look: Step 6 in the presence of kanamycin?
Exercise 2: Transformation
Procedure Animation a. Plate I
Exercise 3: Place the b. Plate II
Stages of Transformation c. Plate III
in Order
d. Plate IV
Analysis of Results I Correct! The correct answer is d, Plate IV.
Label the Results of Your
Experiment 3. If a student wants to verify that transformation has occurred, which of the following
procedures should she use?
Lab Quiz I
a. Spread cells from Plate I onto a plate with LB agar; incubate.
6-II DNA ELECTROPHORESIS
b. Spread cells from Plate II onto a plate with LB agar; incubate.
Key Concepts II c. Repeat the initial spread of kanR cells onto plate IV to eliminate possible
Concept 1: How Do experimental error.
Restriction Enzymes Work? d. Spread cells from Plate II onto a plate with LB agar with kanamycin; incubate.
Concept 2: Cutting DNA e. Spread cells from Plate III onto a plate with LB agar and also onto a plate with LB
with Restriction Enzymes agar with kanamycin; incubate.
Concept 3: Gel
Electrophoresis Correct! The correct answer is d.

Design of the Experiment II 4. During the course of an E. coli transformation laboratory, a student forgot to mark the
Exercise 1: Preparing the culture tube that received the kanamycin-resistant plasmids. The student proceeds with the
Gels laboratory because he thinks that he will be able to determine from his results which
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Exercise 2: Loading the Gel culture tube contained cells that may have undergone transformation. Which plate would
Exercise 3: Filling the Wells be most likely to indicate transformed cells?
Exercise 4: Electrophoresis
Exercise 5: Running the Gel a. A plate with a lawn of cells growing on LB agar with kanamycin
Exercise 6: Staining and b. A plate with a lawn of cells growing on LB agar without kanamycin
Photographing the DNA c. A plate with 100 colonies growing on LB agar with kanamycin
d. A plate with 100 colonies growing on LB agar without kanamycin
Analysis of Results II
Making a Standard Curve Correct! The correct answer is c.
for Hindlll DNA Fragments
Making a Standard Curve Use the following information to answer questions 56.
Practice Problem 1
Practice Problem 2 A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It
could have been kanamycin, ampicillin, or tetracycline. She decides to make up a special set
Lab Quiz II of plates to determine the type of antibiotic used. The plates below show the results of the
test.

5. Which antibiotic plasmid has been used?

a. kanamycin
b. ampicillin
c. tetracycline

Correct! The correct answer is c.

6. What is the explanation for these results?

a. Plates I and II each contain a plasmid that is resistant to that antibiotic.

b. Plate III has antibiotic agar, but E. coli that has been transformed to be resistant
to tetracycline can grow.
c. Plate IV has no antibiotic.
d. There are no tetracycline-resistant cells on Plate II.

Correct! In the second part of this laboratory, you will learn how to separate and analyze
DNA fragments.

Check Your Answers

Next

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