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SM 385. Part 9000 Title Page

This document outlines the coordinators and chairs for the joint task group for the 23rd edition of Standard Methods for the Examination of Water and Wastewater. It provides a summary of major changes since the 2012 edition, including expanded quality assurance/quality control procedures, updated sample collection and storage information, and revisions to procedures for detecting various microorganisms like coliforms, E. coli, and fungi.

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David Amaya
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195 views2 pages

SM 385. Part 9000 Title Page

This document outlines the coordinators and chairs for the joint task group for the 23rd edition of Standard Methods for the Examination of Water and Wastewater. It provides a summary of major changes since the 2012 edition, including expanded quality assurance/quality control procedures, updated sample collection and storage information, and revisions to procedures for detecting various microorganisms like coliforms, E. coli, and fungi.

Uploaded by

David Amaya
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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PART 9000

MICROBIOLOGICAL EXAMINATION

Part Coordinators
Ellen B. Braun-Howland and Margo E. Hunt

https://doi.org/10.2105/SMWW.2882.178
JOINT TASK GROUP CHAIRS FOR THE 23RD EDITION

9020 Quality Assurance/Quality Control..........................................................................Margo E. Hunt


9030 Laboratory Apparatus .....................................................................................................Gil Dichter
9040 Washing and Sterilization ........................................................................................Margo E. Hunt
9050 Preparation of Culture Media...................................................................................Margo E. Hunt
9060 Samples ...........................................................................................................................Gil Dichter
9215 Heterotrophic Plate Count.................................................Gil Dichter and Mark W. LeChevallier
9221 Multiple-Tube Fermentation Technique for
Members of the Coliform Group.......................................................................Ellen B. Braun-Howland
9222 Membrane Filter Technique for Members of the Coliform Group .........................Nancy H. Hall
9223 Enzyme Substrate Coliform Test ................................................................................Jennifer Best
9230 Fecal Enterococcus/Streptococcus Groups ............................................................Rachel T. Noble
9250 Detection of Actinomycetes .....................................................................................Margo E. Hunt
9610 Detection of Fungi....................................................................................Joseph O. Falkinham, III

SUMMARY OF MAJOR CHANGES SINCE 2012

Quality Assurance/Quality Control (9020) has been expanded to address requirements for regulatory
compliance, along with updates on personnel training, biosafety, and temperature-sensing devices.
The language was clarified in Laboratory Apparatus (9030), Washing and Sterilization (9040), and
Preparation of Culture Media (9050). Samples (9060) contains new information on sample collection,
preservation, and storage.
Heterotrophic Plate Count (9215) contains new information on sample preparation and agar use, as
well as summarizing incubation times and temperatures for media.
The Multiple-Tube Fermentation Technique for Members of the Coliform Group (9221) now
includes quality control (QC) information and a procedure for the simultaneous determination of
thermotolerant coliforms and E. coli. The procedure for the confirmed phase of the Presence–Absence
test was rewritten.
The Membrane Filter Technique for Members of the Coliform Group (9222) includes new QC
information and new laboratory apparatus lists. MF Partition Procedures (formerly 9222G) has been
split into Partitioning Thermotolerant Coliforms from MF Total Coliform using EC Broth (now
9222G), Partitioning E. coli from MF Total Coliform using EC-MUG Broth (9222H), and Partitioning
E. coli from MF Total Coliforms using NA-MUG Agar (9222I). Simultaneous Detection of Total
Coliform and E. coli by Dual-Chromogen Membrane Filter Procedure (formerly 9222H) is now 9222J.
Simultaneous Detection of Total Coliforms and E. coli by Fluorogen/Chromogen Membrane Filter
Procedure (formerly 9222I) is now 9222K.
Enzyme Substrate Coliform Test (9223) now includes information on sample collection and QC.
Also, incubation times and temperatures were clarified—including instructions for pre-warming sam-
ples—and specific directions for analyzing samples in various MPN formats are given.
Fecal Enterococcus/Streptococcus Groups (9230) includes new QC procedures to test each lot of
commercial media. Editorial revisions were made to Detection of Actinomycetes (9250) and Detection
of Fungi (9610), and references were updated in 9610.

https://doi.org/10.2105/SMWW.2882.178

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