Research Inverted Microscope

IX83/IX73/IX53
IX3 Series

Built for Live Cell Imaging

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Class A device). Using it in a residential environment may affect other equipment in the
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owners.
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• Specifications and appearances are subject to change without any notice or obligation on the part
of the manufacturer.

Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan 21 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia

Wendenstrasse 14-18, 20097 Hamburg, Germany 5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.

3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A. A8F, Ping An International Financial Center, No. 1-3, Xinyuan South Road,
Chaoyang District, Beijing, China, 100027
491B River Vallay Road, #12-01/04 Valley Point Office Tower, Singapore 24837
Printed in Japan M1756E-062012
 AdvAnce to A HIgHer LeveL of LIve ceLL
reSeArcH, WItH tHe IX3
The new IX3 is a highly expandable platform for live cell imaging
designed with the scientist’s workflow in mind.

Built on a robust foundation and able to grow as your needs evolve,

the IX3 features flexible construction with an easy-access light-path and
offers high-definition wide-field imaging with minimal loss of light.

Equipped with a camera, the IX3 provides fast user-friendly,

high resolution digital imaging with high reproducibility.

1 2
 System overview

eXpAndAbLe to groW WItH Your reSeArcH

IX83: Two-deck System IX83: One-deck System

Enables high-speed, fully automated device selection during An intelligent, motorized microscope that can be equipped with
live cell research including time-lapse imaging. Two decks the IX3-ZDC to create a new standard for live cell imaging
offer maximum expandability.

IX73: One-deck System IX53: One-deck System

The fully-motorized and automated IX83 along with the semi-motorized IX73, are designed to satisfy a myriad of
research needs. Each model is available as a one-deck system with ergonomic low stage height or as a two-deck
system. With additional modules providing expanded functionality, both microscopes provide the ability to enable
a multitude of imaging techniques, ranging from long-term time lapse imaging and other demanding cutting edge
techniques to casual documentation. No matter what the task, the IX3 series delivers the performance and
expandability needed to accommodate the demands of tomorrow.

The left frame port on the IX83 provides ready access to the light-path making it easy to add or change modules.
A variety of deck modules may be easily changed to supply added functions. Modules available include:
Fluorescence filter turrets, side ports, magnification changer and more. Modules can be mounted with ease and
allow users to build a single, uniquely flexible system. IX3-ZDC module with its own specialized port is available for
IX83 systems to maintain continuous focus throughout extended time-lapse use. A microscope designed with emphasis on working efficiency An outstanding microscope delivering cost efficiency for
for documentation, routine testing, and other tasks. brightfield and fluorescence applications.

*Besides IX3-ZDC
3 4
 Impressive Image Quality

reLIAbLe HIgH reSoLutIon ImAgeS tHAt Are

cLeAr And brIgHt

excellent Image
Apochromatic objectives enable High-resolution observation of phase contrast Silicone objectives* enable High-reso-
and fluorescence lution observation of deep Live cells
Phase contrast apochromatic objectives (UPLSAPO100×OPH, PLAPON60×OPH) enable These high-NA objectives (UPLSAPO30×S
high-precision observation free from optical axis displacement—even during simultaneous and UPLSAPO60×S) use silicon oil as an
observation of phase contrast and fluorescence, negating the need to change the objective immersion medium with a refractive in-
when switching methods. dex (ne≈1.40) close to that of living tissue
(ne≈1.38) normally investigated in live cell
imaging. As a result, these objectives deliver
high-resolution observation with minimal
spherical aberration that is commonly
caused by a refractive index mismatch
when viewing deep
Fly-eye Lens inside living tissue.

HeLa cells

Special objective Available for ipS/eS and floating cell observation bright, uniform fluorescent Illumina-
This high-NA phase contrast objective (UCPLFLN20×PH) is especially suited for the observa- tion
tion of plastic dishes. It enables phase contrast observation of the cell proliferation process, The fluorescence illuminator (IX3-RFALFE)
for example, and delivers differentiation across a wide area in high resolution. incorporates a fly-eye lens system to pro-
vide an even distribution of fluorescence
illumination.

Surface of a Fly-eye Lens

System, Enlarged Image
Maximum field number: 22 (IX83P1ZF, IX73P1F), 20 (IX73P2F), 18 (IX83P2ZF) Phase contrast image of mouse Immunofluorescence staining
ES cells for Nanog

Olympus UIS2 infinity-corrected optics provide bright, high resolution images from ultraviolet to near-infrared.

The system ensures high optical transmittance with a broad range of objectives providing wide chromatic correc- High S/n fluorescent mirror units that efficiently detect fluorescence Signals Led transmitted Illumination
All fluorescence mirror units feature filters treated with a coating specially developed to With its perfect color reproduction and low
tion and high resolution, as well as high S/N primary images regardless of the observation method. The wide field minimize noise by absorbing more than 99% of stray light, while the sharp performance and heat generation, this LED illumination is
of view and fly-eye lens system provide uniform fluorescence images and enable the use of sCMOS cameras with high transmittance of the mirror units ensure efficient fluorescence signal detection. ideal for time-lapse imaging.
large chips. U-MWU2 U-FUW
100 100
90 90
80 80 LED Transmitted

Transmittance (%)
Transmittance (%)
70 70 Llight Illuminator
60 60
50 50
40 40
30 30
20 20
10 10
0 0
300 350 400 450 500 550 600 650 700 750 800 300 350 400 450 500 550 600 650 700 750 800
Wavelength (nm) Wavelength (nm)
BP330–385 BA420 DM400 BP340–390 BA420IF DM410

IX2 Series IX3 Series

*Use dedicated silicone oil.

5 6
 ease of use

IntuItIve And ergonomIc controL over

mIcroScope performAnce

Smart control operator-friendly design

Switch observation methods with a Smooth tracking at High magnification
tap of the touch panel The IX3-SVR manual stage features a
A press of a touch panel is all it takes to smooth positioning system which enables
change observation settings, with auto- the easy tracking of cells even in high
matic controls enabling switching of optical magnifications. The user settable position
components, aperture adjustment, and limits immobilizes the stage, ensuring that
exposure. the observation position is maintained dur-
ing operations such as reagent application,
even if the stage is inadvertently touched.
It is also possible to remove 35-mm dishes
from the stage, place them in an incubator
for culturing, and return them to the stage—
repositioning the exact location of the cells
Zdc one-shot function detects focus fast, even in High magnification within the
observation field of view.
IX3-ZDC focus detection and tracking can be performed via the innovative touch panel
independent of software. There's also a focus search function supported by a cell-safe,
near-infrared laser enabling instant focusing on samples—even at high magnification.

Koehler Illumination control via the

frontal condenser Knob
Using a condenser lock and the front-
Just Touch the Panel For Instant Focus
located control knobs, the condenser can
be moved
and easily
reset to
Intuitive microscope and XY Stage Koehler
controllers Illumination.
The combination of the U-MCZ and XY-
controller make it possible to provide the
familiarity of conventional handle operation
for confident working even in a darkroom.
The IX3 imaging system incorporates a range of advanced technologies to enable fine control of your imaging. As
a result, it allows researchers to refine complex sequences of operations into workflows with speed and comfort,
eliminating burden on the observer and minimizing cell damage. frame construction prevents optical
System contamination
A repositionable controller can be located comfortably close to hand, while Olympus cellSens imaging software A catch tray under the nosepiece prevents
enables advanced control of functions. There is also an innovative, user-friendly touch panel that makes digital microscope configuration recall damage to the microscope frequency
(olympus cellSens) caused by contamination through spilled
control simple and accurate, even when working under darkroom conditions. The IX3-ZDC Z-drift compensation The system saves microscope configura- liquids and
system, employs a near-infrared light to minimize cell damage while enabling instant focus. tions alongside image data through incor- simplifies
poration of a readout function that utilizes maintenance.
motorized units and coded units. With this
advanced system, a wide range of set-
tings can be recalled at will to recreate the
desired imaging conditions, thus creating an
easy to use reproducible high end imaging
Olympus cellSens Imaging Software
system.

7 8
 reliable data Acquisition

IdeAL obServAtIon And cApture of

tIme-LApSe ImAgeS

Accuracy
Z-drift compensation System maintain cell viability over an extended
IX3-Zdc optical path diagram
The IX3-ZDC uses low phototoxicity IR light period of time
to detect the correct focus position as set Box-type incubator enables time-lapse
cell
by the user. One-shot AF mode allows sev- observations over a period of several days,
eral focus positions to be set as desired for Offset while the microscope CO2 incubator can be
deeper samples, enabling efficient Z-stack fitted to the stage for two-day time-lapse
Offset lens
acquisition in multi-position experiments. observations maintaining cell activity to sig-
Cover glass
Continuous AF mode keeps the desired Objective nificantly improve the reliability of time-lapse
plane of observation precisely in focus, AF sensor
observation.
avoiding focus drift due to temperature
changes or the addition of reagents, making
it ideal for measurements such as TIRF that
have requirement more stringent focusing.

▼ time-lapse observation Images using Zdc example

low

co2 Stage top Incubator

Precise controls maintain a constant envi-
Cantilevered ronment within the dish or well plate con-
The cantilevered structure with  Drive Guide high trolling thermally, humidity, and CO2 con-
a Z drive guide located near the Apoptosis in cultured human ES cells, photographed at 2-minute intervals over 5 hours. centration.
nosepiece offers high resistance (Top row: DIC imaging of physical changes; Bottom row: FRET imaging of Caspase-3 action)
to thermal drift.
Image data courtesy of:
Masatoshi Ohgushi, Ph.D.
Yoshiki Sasai M.D., Ph.D.
Human Stem Cell Technology Unit, RIKEN Research Center for Developmental Biology
Reference material:
Ohgushi, M. et al. Molecular Pathway and Cell State Responsible for Dissociation-Induced Apoptosis in Human
Pluripotent Stem Cells. Cell Stem Cell 7, 225-239(2010).

With new frame architecture and focus drive design, the IX3 system offers enhanced rigidity that reduces the impact IX3D CO2 Incubator

of vibration and heat. It maintains desired positions along X, Y, and Z axes to allow reliable time-lapse imaging.
The real-time Z-drift compensation system capabilities of the IX3-ZDC combine with the Olympus ultrasonic stage-
capable of multipoint imaging to enable capturing high-precision multipoint time-lapse images that are never out-of- Low thermal drift ultrasonic Stage Incubator
with multipoint registration A box-type incubator keeps the microscope
focus or misaligned. The Olympus ultrasonic stage enables temperature stable while safely enclosing
precise movement of specimens, while many components.
Box-type and onstage incubators are also available to enable time-lapse observation while maintaining the viability of multi-point registration enables time-lapse
live cells. imaging with outstanding positional
IX81 accuracy.
IX81

IX83

IX83

Square Frame for

0 50 100 150 200 (min) 0.0 0.5 1.0 1.5 2.0 (s)
Increased Rigidity
Thermal Drift Displacement Periodic Damping

9 10
 modularity and expandability

SopHIStIcAted ImAgIng optIonS WItH

IntercHAngeAbLe optIcAL moduLeS

deck units/fast Speed units fluorescence System

motorized fluorescence reflected Light fluorescence Illuminators for Your Specific
mirror turret (IX3-rfAcA) Application
A non-click turret fitted with 8 Choose the illuminator best suited to meet each need, such as
mirror units delivers smooth, multicolor fluorescence observation or photoactivation. An L-shaped
fast switching. Mirror units can fluorescence illuminator with a fly-eye lens system provides bright,
be used with 25-mm diameter consistent illumination without adjustment, an L-shape-fluorescence
filters or 32-mm diameter illuminator is equipped with a field iris diaphragm and aperture iris di-
filters. No tool use is required to aphragm, and a straight-through fluorescence illuminator is available
change mirror units, which can for applications demanding intense excitation light. A wide range of
be opened with ease. light sources are available, including light-guide light sources and
IX3-RFACA lamp houses compatible with both 100-W mercury and 75-W xenon
right Side port with c-mount illumination.
(IX3-rSpc) 1U-LH100HG
A right side port with a C-mount 2 2U-LH75XEAPO
(field number of 11) allows the 3 4 3U-LH100HGAPO
light-path switching component 1 4U-HGLGPS
to be fitted with up to two mirror 5IX3-RFA
units, enabling the construction
6U-LLGAD
of customized systems for
7IX3-RFAL
applications such as split 5 8IX3-RFALFE
imaging. 6
IX3-RSPC
coded Intermediate
magnification changer 7
(IX3-cAS) 8
Magnification can be changed
between 1x, 1.6x, and 2x by
smooth lever operation. Since
the system incorporates coded
functionality, information on motorized units/coded units
intermediate magnifications is
saved with image data. A cost-efficient Way to upgrade to a motorized microscope
IX3-CAS A wide range of motorized and encoded units are available,
including an 8-position motorized fluorescent mirror turret, an
motorized fast filter Wheel encoded fluorescence mirror turret, a motorized 6-position
(u-ffW)/ nosepiece, an encoded 6-position nosepiece, a motorized long
A diverse range of units is available for the Olympus IX3 microscope system, bringing greater efficiency to every- motorized fast filter Wheel working distance universal condenser, filter wheels, and shutters.
for emission (u-ffWem)/ Units can also be added for specific purposes at any point—
thing from casual observation to serious imaging. Simple cassette-like insertion into the deck makes it easy to motorized fast Shutter improving operation at a minimum cost.
mount fluorescence mirror turrets, a right side port with C-mount, an encoded magnification changer, reflected light (u-fSHu) U-FFW 1U-FFWEM
Filter wheels can be switched
fluorescence illuminators and other desired units. 2IX3-LWUCDA
between filters in just 60 mil- 4
3 3U-FFW
liseconds, while shutters can be
To provide more flexibility, the two-deck system allows the simultaneous mounting of two illumination units. The opened and closed in just 26 2 4U-FSHU

IX3 system can also be isolated from vibration sources through the combined use of a new line of high-speed filter milliseconds. The IX83 is 1
5IX3-RFACA
5 6IX3-D6RES
wheels and light guide light sources. Upgrades are available to allow the IX3 to meet the needs of a wide range of capable of controlling up to six
filter wheels and four shutters. U-FFWEM 7IX3-D6REA
other applications.

7
U-FSHU

11 12
 objective specifications
Water proof &
W.D. Cover glass Correction Iris
optImIZed ImAge AcQuISItIon AccordIng to
UIS2 objective NA FN Immersion Spring oil proof
(mm) thickness (mm) ring diaphragm
function
UPLSAPO UPLSAPO 4× 0.16 13 26.5 —
WorKfLoW UPLSAPO 10×2 0.40 3.1 26.5 0.17
UPLSAPO 20× 0.75 0.6 26.5 0.17 ✓
UPLSAPO 20×O 0.85 0.17 26.5 — Oil ✓ ✓
UPLSAPO 30×S 1.05 0.8 22 0.13–0.19 Silicone ✓ ✓
UPLSAPO 40×2 0.95 0.18 26.5 0.11–0.23 ✓ ✓
UPLSAPO 60×W 1.20 0.28 26.5 0.13–0.21 Water ✓ ✓ ✓
UPLSAPO 60×O 1.35 0.15 26.5 0.17 Oil ✓ ✓
UPLSAPO 60×S 1.30 0.3 22 0.15–0.19 Silicone
olympus cellSens Imaging Software ✓ ✓ ✓ ✓
UPLSAPO 100×O 1.40 0.13 26.5 0.17 Oil ✓ ✓
2d deconvolution UPLSAPO 100×OPH 1.40 0.13 26.5 0.17 Oil ✓ ✓
This useful feature eliminates blur from PLAPON PLAPON 60×O 1.42 0.15 26.5 0.17 Oil ✓ ✓
single plane images. Processing can be PLAPON 60×OSC 1.40 0.12 22 0.17 Oil ✓ ✓
carried out multiple times and the results PLAPON 60×OPH 1.42 0.15 26.5 0.17 Oil ✓ ✓
can be adjusted while viewing them on the UPLFLN UPLFLN 4× 0.13 17 26.5 —
screen. A 3D deconvolution module UPLFLN 10×2 0.30 10 26.5 —
is also available for UPLFLN 20× 0.50 2.1 26.5 0.17
Original image ✓
use on multi-plane
UPLFLN 40× 0.75 0.51 26.5 0.17 ✓
images.
UPLFLN 40×O 1.30 0.2 26.5 0.17 Oil ✓ ✓
UPLFLN 60× 0.90 0.2 26.5 0.11–0.23 ✓ ✓
UPLFLN 60×OI 1.25–0.65 0.12 26.5 0.17 Oil ✓ ✓ ✓
Deconvolved image UPLFLN 100×O2 1.30 0.2 26.5 0.17 Oil ✓ ✓
UPLFLN 100×OI2 1.3-0.6 0.2 26.5 0.17 Oil ✓ ✓ ✓
PLFLN PLFLN 100× 0.95 0.2 26.5 0.14–0.2 ✓ ✓
Olympus cellSens imaging software is available in three packages Well navigator UCPLFLN UCPLFLN 20× 0.7 0.8–1.8 22 0–1.6 ✓
to meet individual workflow needs. "Entry" is used for simple image Enables the simple setting of locations and UCPLFLN 20×PH 0.7 0.8–1.8 22 0–1.6 ✓
acquisition. “Standard” provides simple operation for imaging docu- observation orders by selecting to the wells LUCPLFLN LUCPLFLN 20× 0.45 6.6–7.8 22 0–2 ✓
mentation, and "Dimension" allows control of the complete workflow under observation. Comments can also be LUCPLFLN 40× 0.60 2.7–4 22 0–2 ✓
from image capture to analysis. inserted for individual wells to further speed
LUCPLFLN 60× 0.70 1.5–2.2 22 0.1–1.3 ✓
cellSens software is not for clinical diagnostic use workflow.
LUCPLFLN 20×PH 0.45 6.6–7.8 22 0–2 ✓
LUCPLFLN 20×RC 0.45 6.6–7.8 22 0–2 ✓
LUCPLFLN 40×PH 0.60 3.0–4.2 22 0–2 ✓
LUCPLFLN 40×RC 0.60 3.0–4.2 22 0–2 ✓
cell tIrf System LUCPLFLN 60×PH 0.70 1.5–2.2 22 0.1–1.3 ✓
UPLFLN-PH UPLFLN 4×PH 0.13 17 26.5 —
nA 1.7 tIrf objective UPLFLN 10×2PH 0.30 10 26.5 —
The NA 1.7 APON100×HOTIRF* objective expands the
UPLFLN 20XPH 0.50 2.1 26.5 0.17 ✓ ✓
adjustable range for production of evanescent fields,
UPLFLN 40XPH 0.75 0.51 26.5 0.17 ✓ ✓
enabling the user to form thin evanescent fields by
simply adjusting the angle of incidence. High NA UPLFLN 60XOIPH 1.25-0.65 0.12 26.5 0.17 Oil ✓ ✓
objectives for TIRF from 60× to 150× are also UPLFLN 100XO2PH 1.30 0.2 26.5 0.17 Oil ✓
available. UPLFLN-PHP UPLFLN 4×PHP 0.13 16.4 22 —
CPLFLN CPLFLN 10×PH 0.30 9.5 22 1
tIrf Image Acquisition with High resolution and a High CPLFLN 10×RC 0.30 9 22 1.5
frame rate LCACHN LCACHN 20×PH 0.40 3.2 22 1
TIRF observation demands more accurate focusing. To meet this LCACHN 20×PHP 0.40 3.2 22 1
demand, the IX83 two-deck system can be combined with the
LCACHN 20×RC 0.40 2.8 22 1.5
IX3-ZDC to deliver live imaging at a high frame rate, while maintain-
LCACHN 40×PH 0.55 2.2 22 1
ing accurate real-time focus.
Image data courtesy of: LCACHN 40×PHP 0.55 2.2 22 1
Akiko Hashimoto-Tane, Ph.D. LCACHN 40×RC 0.55 1.9 22 1.5
Takashi Saito, Ph.D. CACHN & CPLN CACHN 10×PHP 0.25 8.8 22 —
Quad-wavelength multiple tIrf Image capture Laboratory for Cell Signaling,
Accurate, motorized control of the laser’s angle of incidence enables RIKEN Research Center for Allergy
CPLN 10×PH 0.25 10 22 1
optimum adjustment of the light emitted at each wavelength. This and Immunology CPLN 10×RC 0.25 9.7 22 1.5
system allows four lasers to be used simultaneously to capture four Reference material: UAPON 340 UAPON 20×W340 0.70 0.35 22 0.17 Water ✓ ✓
different wavelengths (ranging from 405 nm to 640 nm), while Akiko Hashimoto-Tane, Takashi
UAPON 40×O340 1.35 0.1 22 0.17 Oil ✓ ✓
Saito, et al. (2011). Dynein-Driven
seamlessly switching between multicolor TIRF imaging and UAPON 40×W340 1.15 0.25 22 0.13–0.25 Water ✓ ✓ ✓
Transport of T Cell Receptor Micro-
fluorescence. What’s more, the primary laser path is equipped with clusters Regulates Immune Synapse TIRF APON 60×OTIRF 1.49 0.1 22 0.13–0.19 Oil ✓ ✓
a point FRAP optical solution system that can also be used for kinet- Formation and T Cell Activation.
UAPON 100×OTIRF 1.49 0.1 22 0.13–0.19 Oil ✓ ✓
ic measurements such as molecular diffusion, bonding, and velocity Colocalization of the Dynein Complex with Immunity 34, 919-931.
determination. cell TIRF is a class 3B laser product. T Cell Receptor Microclusters •Images shown left acquired by IX81. UAPON 150×OTIRF 1.45 0.08 22 0.13–0.19 Oil ✓ ✓
APON 100×HOTIRF* 1.70 0.08 22 0.147–0.153 Oil ✓ ✓
*HIGHINDEX-CG cover glass and dedicated immersion oil required
* Requires special cover glass and immersion oil.
13 14
IX83/IX73 system diagram

Observation tubes, eyepieces Transmitted illumination 45FR Controllers I

45-LBD-IF
45-ND25 U-LH100L-3
WHN10× IX-ADUCD
Condenser adapter
45-ND6
43IF550W45
100 W halogen III
WHN10×-H lamp housing
B Filters
Eyepieces A
XIII IV
U-CT30-2 U-BI90 U-TBI90 U-TR30-2, U-TR30H-2 C1394A
Optical U-UCD8-2 IX3-CBH
Centering
telescope Binocular tube Tilting U-TR30NIR Trinocular elements Universal condenser TH4-HS Control box Interface cable V
binocular tube Trinocular tube Hand switch
tubes U-RMT
U-LH100-3 Extension cord TH4-100
U-TLO U-LHEAD 100 W halogen Power supply unit U-IFCBL100
Oil top lens Extension adapter lamp housing Interface cable,
IX-ATU U-CBFCBL
Intermediate tube for lamp housing
XI 100 cm CBF cable VI
U-TLD
Dry top lens
7
C IX3-ILL
Transmitted
XII U-CBF VI U-CBF
IX3-LHLEDC Control box for Control box for
illuminator LED lamp U-IFCBL200 fast filter wheel fast filter wheel
house
U-LEDPS
VII Interface cable,
200 cm
and shutter and shutter
GX-SPU U-EPA2 U-CA U-ECA LED power supply U-IFCBL15
Side port
intermediate
Eyepoint
adjuster
Magnification changer
1x, 1.25x, 1.6x, 2x
Magnification
changer 2x
Optical
elements 3 VI Interface cable,
15 cm U-HSCBM
Hand switch
tube IX2-MLWPO IX2-MLWCD U-FSHU
1 Polarizer attachment Mid long working Motorized fast shutter VII
distance condenser X IX3-CBM cellSens
II U-NPCBL100
Interface cable,
Control box M RS232C Imaging
software
U-IFCBL100 100 cm Interface cable
Stages Incubator Interface cable, 100 cm VIII
IX-HOP IX-HOS IX-HOT IX-CLMT, IX-PPM IX-LWPO U-AW
VII XIII IX
Petri dish Slide glass Terasaki IX-CLM96 Scale for Polarizer attachment IX2-LWUCD U-IFCBL200
holder holder plate holder Well positioners NUNCTM plate Optical
Long working
distance universal
Motorized
attenuator wheel Interface cable, 200 cm X U-CB5S
Control box for coded function
elements condenser

Incubator Fluorescence illuminators VI VI VI

(Available in the near future.)
IX-MVR U-FMP
II IX3-CAS
Coded intermediate
Mechanical Mechanical stage U-IFCBL100 U-FFW U-FFW U-FSHU
magnification changer IX3-RFA Motorized Motorized Motorized fast shutter
stage Interface cable,
IX3-LWUCDA Fluorescence fast filter wheel fast filter wheel
100 cm
Motorized LWD VIII illuminator 25ND1.5
8 VII
condenser U-COCBL100 25ND6 III
CODE cable 25ND25 U-IFCBL100
U-IFCBL200 II 25ND50 Interface cable, 100 cm
Interface cable, IX3-FDICT U-IFCBL15 Filters
200 cm Transmitted Interface cable, U-AW
Motorized attenuator wheel
VII
IX3-HO35D IX3-HOS DIC filter unit 15 cm
Dish holder Slide holder Optical U-IFCBL200
elements IX3-RFACA Interface cable, 200 cm
Motorized fluorescence VII IX3-RFAL
L-shape-
U-LHEAD
Extension
IX-ULWCD mirror turret U-IFCBL100 fluorescence
Ultra long working IX3-FFXL adapter
IX3-FCFPXL Interface cable, illuminator for lamp
distance condenser 100 cm XLED1
IX3W/IX3D IX3-FGFPXL housing Microscope
CO2 incubator IX3-FYFPXL adapter High intensity
(Stage adapters IX3-MCHEXL LED fluorescence
separately required. IX3-FUWXL IX3-RSPC microscope light source
Available in the near future.) IX3-FGWXL Right side port with U-RFSS (Available in the near future.)
C-mount Rectangular
U-RFL-T
LIFE TIME

Thermoplate IX3-FBVWXL
(Available in the near future.) XL mirror units field stop U-RFL-T
IX3-HOW IX-CP50 IX2-GCP
BURNER ON

Microplate Insert plate Glass stage (ø32) Power supply

holder insert plate unit for
mercury lamp
U-ANT U-DULHA U-LH100HG
Mirror units IX3-RFACS 100 W mercury lamp housing
Coded fluorescence Analyzer for IX3-RFALFE Double lamp
mirror turret transmitted L-shape- housing U-LH100HGAPO U-CLA
U-RFL-T
LIFE TIME

light fluorescence adapter 100 W mercury apo lamp housing Collector lens
illuminator with U-RX-T
VIII
BURNER ON

adjustment Power supply

IX-SCL flyeye lens
Slide clip U-COCBL100 unit for
CODE cable U-DICT xenon lamp
IX-SCL IX-SCL DIC slider for U-LH75XEAPO
Slide clip Slide clip XY-controller transmitted light 75 W xenon apo lamp housing
(attached to
BX3-SSU)
Revolving U-HGLGPS
U-LLGAD U-LLG150 U-FFWADP
CK2-SS IX2-SP BX3-SSU
nosepiece Liquid light Liquid light Adapter
100 W
mercury
IX3-SVR IX2-GS GX-SVR GX-SFR guide guide (1.5 m) for light guide lamp housing
Side stage Plain stage Mechanical Gliding stage Right handle Flexible right Scanning stage Control box adapter illumination with fiber
with ultrasonic U-DICTS U-LLG300
stage with stage for GX handle stage (attached to BX3-SSU) Shift DIC slider for system
right handle Liquid light
(Insert plate (Exclusive transmitted light guide (3 m)
separately unit for IX73. cell TIRF
required.) Insert plate
separately
V IX3-D6RES
Coded 6 position 5 U-DICTHC
High contrast
DIC slider for
Motorized TIRF system
(Cannnot be used with IX83P1ZF.
nosepiece
required.) IX transmitted light Cannnot be used with other illuminators.
2 U-DICTHR 4 Available in the near future.) U-FFW U-FFW
XI U-NPCBL100 High resolution Motorized
fast filter wheel
Motorized
fast filter wheel U-FSHU
Interface cable, DIC slider for
100 cm Motorized fast
transmitted light shutter
Camera adapters, camera ports
D
VI VI VI
U-BMAD U-CMAD3 U-SMAD U-TMAD U-CMT U-FMT
Bayonet C-mount Sony T mount C-mount F mount adapter IX3-ZDC
mount adapter mount adapter adapter IX3-D6REA
Motorized 6 position 2 5 3 Z drift compensator 2 3
adapter adapter
VII nosepiece
D IX-TVAD
Primary image XII U-IFCBL200
camera port tube Interface cable,
U-TV1×-2 U-TVZ Cube cassette 200 cm
B
Primary image Zoom camera for full image/ II II
camera port
tube
port tube split images 1 1 4 III
VI (Not available
in some areas.) IX3-AN
U-MCZ
Analyzer slider
Controller
C
U-SIP
4 7
Split primary
U-TV1×C
C-mount camera
U-TV0.63×C
C-mount
U-TV0.5×C-3
C-mount
U-TV0.35×C-2
C-mount
U-TV0.25×C
C-mount
U-FFWEM
Motorized fast
U-DPCAD
Dual port
image camera port IX73P2F IX83P2ZF IV
adapter 1x camera camera camera camera (Not available IX73 2port frame IX83 2port frame Touch panel controller
filter wheel tube with in some areas.)
(XY adjustment) port tube with port tube with port tube with port tube with for emission C-mounts IX73P1F IX83P1ZF (attached to IX83P2ZF/IX83P1ZF)
C 0.63x lens C 0.5x lens C 0.35x lens 0.25x lens IX73 1port frame IX83 1port frame
A
Exclusive units for IX83
A 6 6 6 8 XIII 6 8 I II IX3-FP
Exclusive units for IX73
Frame fix plate

15 16
 IX3 specifications dimensions (Unit : mm)
IX83 IX73 IX53
IX83: Two-Decks IX83: One-Deck
Optical system UIS2 optical system (IX83P2ZF configuration) 363 (IX83P1ZF configuration) 366

• Motorized sextuple revolving nosepiece

(DIC slider attachable),
• Motorized sextuple revolving nosepiece
simple waterproof structure • Sextuple revolving nosepiece,
Revolving nosepiece (DIC slider attachable),
• Coded sextuple revolving nosepiece simple waterproof structure
simple waterproof structure
(DIC slider attachable),
Microscope frame simple waterproof structure

751

686
Stroke: 10.5 mm
Minimum increment: 0.01 μm,

433
Focus Stroke: 10 mm Stroke: 10 mm

430
Maximum nosepiece movement speed:

288
288
3 mm/s

Motorized 0:100/50:50/100:0
Light path selection 0:100/50:50/100:0 (Left side port: BI port) 50:50 (Left side port: BI port)
(Left side port: BI port) 145 475 145 475
323 323 568
636 636
•30W halogen illuminator: 769
Detachable condenser lens system
Weight: approx. 54 kg Power consumption: approx. 530 W Weight: approx. 47 kg Power consumption: approx. 370 W
(NA 0.3, W.D. 72 mm), single filter holder
Pillar tilt mechanism (30° inclination angle, with vibration reducing mechanism),
aperture iris diaphragm adjustable
Condenser holder (with with 88 mm stroke, refocusing mechanism),
•100W halogen illuminator:
Field iris diaphragm adjustable,
Transmitted light illuminator Pillar tilt mechanism (30° inclination angle,
4 filter holders
with vibration reducing mechanism), IX73: Two-Decks IX73: One-Deck
Light source: •12 V 100 W halogen bulb (pre-centered)
Condenser holder (with 88 mm stroke, (IX73P2F configuration) (IX73P1F configuration)
• High color reproductive LED light source
refocusing mechanism) 365 365
Field iris diaphragm adjustable,
4 filter holders

•Widefield tilting binocular

Observation tube Widefield (FN 22) •Widefield tilting binocular •Widefield binocular •Widefield trinocular
•Widefield binocular

Scanning stage with ultrasonic Stage stroke: X: 76 mm x Y: 52 mm, maximum stage movement speed: 30 mm/s —

721

656
Mechanical stage with right
114 mm (X) x 75 mm (Y) stroke, stage position locking function
handle

432

432
Right handle stage 50 mm (X) x 50 mm (Y) stroke —

288
Stage
Flexible right handle stage — 50 mm (X) x 50 mm (Y) stroke —
145 475 145 475
Gliding stage Upper circular stage 360° rotatable, 20 mm (X/Y) travel 323 569 323 691
632 509
Plain stage 232 mm (X) x 240 mm (Y) stage size, stage insert plate exchangeable (ø110 mm)
Weight: approx. 41 kg Power consumption: approx. 310 W Weight: approx. 35 kg Power consumption: approx. 310 W
W.D. 27mm, NA 0.55, motorized turret with 7 position slots for optical devices
Motorized long working
(3 positions for ø30 mm and 4 positions for ø38 mm), —
distance condenser
motorized aperture and polarizer

Long working distance IX53: One-Deck Touch panel controller

Condenser NA 0.55, W.D. 27 mm 5 positions for optical devices (3 positions for ø30 mm and 2 position for ø38 mm)
universal condenser (IX53P1F configuration)
365
Long working distance
NA 0.5, W.D. 45 mm, 4 positions for optical devices (for ø50 mm, Relief Contrast optical devices rotatable)
relief contrast

Ultra long working distance NA 0.3, W.D. 73mm, 4 positions for optical devices (for ø29 mm)

L-shape-fluorescence
L-shaped design with exchangeable FS module —
illuminator with flyeye lens

657
Fluorescence illuminator L-shape-fluorescence

104.5
L-shaped design with exchangeable FS and AS modules —
illuminator

432

25º
289
Fluorescence illuminator Straight design with field iris diaphragm
190 210.5
Motorized fluorescence
Motorized turret with 8 positions, built-in shutter, simple waterproof structurer —
mirror turret 145 475
323 691
509
Coded fluorescence mirror Coded 8 positions turret,
Fluorescence mirror turret — —
turret built-in shutter, simple waterproof structure Weight: approx. 32 kg Power consumption: approx. 300 W
Turret with 8 positions,
Fluorescence mirror turret — — IX3-CBH
built-in shutter, simple waterproof structure

•130 W Hg light guide illumination •100 W Hg apo lamp housing and transformer •100 W Hg lamp housing and transformer
Fluorescence light source
•75 W Xe lamp housing and transformer U-HSCBM TH4-100
Offset method (Focus search, one-shot focus,
Focus compensator Z drift compensator —

214
210
continuous focus)

Motorized fast filter wheel High speed mode 60 ms, Low vibration mode 100 ms (rotation time until next hole on the wheel) — 7~
32
Motorized fast filter wheel High speed mode 60 ms, Low vibration mode 100 ms (rotation time until next hole on the wheel)
— U—HSCBM
100 280
Filter wheel/shutter for emission C-mount adapter and bayonet mount adapter are enclosed OPERATION
ATTENUATOR
OBSERV ATION
DF DIC

MEMORY 294.5

120
109

125
Motorized fast shutter High speed mode 26.2 ms, Low vibration mode 60 ms (rotation time on one way) —
MIRROR PO PH

FL SHUTTER

OBJECTIVE BF FL
Voltage/current: AC 100-120 V 50/60 Hz 1.8 A
Motorized attenuator wheel Time to shift another filter 300ms (rotation time until next hole on the wheel) —
146 75 200
• Indoor use 14.5
• Ambient temperature : 5 º to 40 ºC (41 º to 104 ºF)
Voltage/current: AC 100-120/220-240 V
Operating environment • Maximum relative humidity : 80% for temperatures up to 31 ºC (88 ºF), decreasing linearly through 70% at 34 ºC (93 ºF), 60% at 37 ºC (99 ºF), to 50% relative humidity at 40 ºC 50/60 Hz 4.6 A/2.8 A
(104 ºF)
• Supply voltage fluctuations : Not to exceed ±10% of the normal voltage
Motorized or coded units are designed for the IX3 series use in industrial environments for the EMC performance (IEC61326-1 Class A device). Using it in a residential environment may affect other equipment in the environment.

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