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Primers For TKO Library NGS

The document describes barcoded reverse primers and universal forward primers used for sequencing. It lists 8 reverse primers with different barcodes and indexes. It also lists 8 forward primers with varying lengths of staggered nucleotides at the 5' end. The primers are used to add adaptors and indexes for sequencing multiple samples together.

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161 views1 page

Primers For TKO Library NGS

The document describes barcoded reverse primers and universal forward primers used for sequencing. It lists 8 reverse primers with different barcodes and indexes. It also lists 8 forward primers with varying lengths of staggered nucleotides at the 5' end. The primers are used to add adaptors and indexes for sequencing multiple samples together.

Uploaded by

fmachour9316
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Barcoded Reverse Primers

Name Barcode Index read Sequence (5’-3’)


R index 2 ACATCG CGATGT CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 5 CACTGT ACAGTG CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 6 ATTGGC GGCAAT CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 8 TCAAGT ACTTGA CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 3 GCCTAA TTAGGC CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 4 TGGTCA TGACCA CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 7 GATCTG CAGATC CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 10 AAGCTA TAGCTT CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC

Universal Forward Primers


Name Stagger Sequence (5’-3’)
F universal (0nt) - AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
TTGTGGAAAGGACGAGGTACCG
F universal (1nt) C AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
CTTGTGGAAAGGACGAGGTACCG
F universal (2nt) GC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
GCTTGTGGAAAGGACGAGGTACCG
F universal (3nt) AGC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
AGCTTGTGGAAAGGACGAGGTACCG
F universal (4nt) CAAC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
CAACTTGTGGAAAGGACGAGGTACCG
F universal (6nt) TGCACC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
TGCACCTTGTGGAAAGGACGAGGTACCG
F universal (7nt) ACGCAAC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
ACGCAACTTGTGGAAAGGACGAGGTACCG
F universal (8nt) GAAGACCC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
GAAGACCCTTGTGGAAAGGACGAGGTACCG

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