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Cinetica Batch Lev

The document contains data from experiments measuring biomass concentration (X in g/L) and substrate concentration (S in g/L) over time. It also includes calculations of reaction rates (rx), maximum biomass production (Prod max biomasa), and maximum product formation (Prod max producto). Graphs plot ln(X) over time and the specific growth rate (um) against the substrate reaction rate (rs).

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Eric Gabriel
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92 views11 pages

Cinetica Batch Lev

The document contains data from experiments measuring biomass concentration (X in g/L) and substrate concentration (S in g/L) over time. It also includes calculations of reaction rates (rx), maximum biomass production (Prod max biomasa), and maximum product formation (Prod max producto). Graphs plot ln(X) over time and the specific growth rate (um) against the substrate reaction rate (rs).

Uploaded by

Eric Gabriel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLS, PDF, TXT or read online on Scribd
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0 1 2 3 5

0.415 0.437 0.603 0.515 0.49 0.635


1 EN 3

Curva de Biomasa Levadura


y= 1.3305x-0.0887

X (g/L) 0.3785794814 0.39511462 0.51987974 0.4537392 0.43494927 0.54393085

CONSIDERANDO LA DILUCION
TIEMPO 0 1 2 3 5 6
X(g/L) 0.3785794814 0.39511462 0.51987974 0.4537392 1.3048478 1.63179256
ln -0.9713292377 -0.92857938 -0.65415775 -0.7902327 0.26608641 0.48967914

6 2.5

5 2

4 Row1.5
13
3 1

0.5
2
0
1 0 5
-0.5
0
0 5 10 15 20 25 30 -1

-1.5
0.797 0.604 0.729 0.847 0.767 0.817
1EN5 1 EN 10

0.66568959 0.52063134 0.61458098 0.70326945 0.64314168 0.68072153

7 8 9 10 23 26
1.99706877 2.60315671 3.07290492 3.51634724 6.43141676 6.80721533
0.69168049 0.95672483 1.12262334 1.25742273 1.86119485 1.91798313

2.5

Row1.5
13
1
Row
0.5 14

0
0 5 10 15 20 25 30
-0.5

-1

-1.5
Volumen gast Muestra Resultado (g/L)
0 0.65 1 5.85
1 0.8 1 7.2
2 0.95 1 8.55
3 1 9
5 1.2 10.8
6 1.2 10.8
7 1.4 12.6
8 1.55 13.95
9 1.55 13.95
10 1.9 17.1
23 2.9 26.1
26 2.9 26.1
1 2 3 4 5 6
A 0.136 0.116 0.118 0.118 0.222
B 0.113 0.383 0.373 0.383 0.4 0.422
C 0.113 0.464 0.446 0.425 0.426 0.42
D
E
F
G
H

SIN
BLANCO 0.27 0.26 0.27 0.287 0.309
0.779 0.699 0.762 0.779 0.633
0 1 2 3 5
Curva Glucosa

y= 0.3547x-0.0763

DILUCIÓN 1/5
X (g/L) 0.97631802 0.94812518 0.97631802 1.02424584 1.08627009

0 2 3 4 6
4.88159008 4.74062588 4.88159008 5.12122921 5.43135044
X (g/L)

X (g/L) 2.41133352 2.18579081 2.36340569 2.41133352 1.99971807

0 1 2 3 5
X (g/L) 24.1133352 21.8579081 23.6340569 24.1133352 19.9971807
0 1 2 3 5
24.1133352 21.8579081 23.6340569 24.1133352 19.9971807
7 8 9 10 11 12
0.195 0.246 0.445 0.659 0.104 Read 2:550

0.333 0.322 0.909 0.367 0.111 Read 2:550

0.395 0.379 0.337 0.166 0.123 Read 2:550

Read 2:550

Read 2:550

Read 2:550

Read 2:550

Read 2:550

0.22 0.209 0.796 0.254 -0.002


0.586 0.597 0.574 0.55 0.49 0.111 0.062
6 7 8 9 10 23 26

SIN DILUCION
0.83535382 0.8043417 2.45926135 0.93120947 0.20947279
ACTIVIDAD ENZIMATICA
7 8 9 11 24
4.1767691 0.8043417 2.45926135 0.93120947 0.20947279 volver a medir
4.02170849 12.2963067 4.65604736 1.04736397
-14.96053 -6.03749648 -13.0011277 -14.9182408

1.86721173 1.89822385 1.83338032 1.76571751 1.59656047 0.52805187 0.38990696


SUSTRATO
6 7 8 9 10 11 23
18.6721173 18.9822385 18.3338032 17.6571751 15.9656047 5.28051875 3.89906964
6 7 8 9 10 11 23
18.6721173 18.9822385 18.3338032 17.6571751 15.9656047 5.28051875 3.89906964

30

25

20

15 Row
34

10

0
20

15 Row
34

10

0
0 5 10 15 20 25
ACTIVIDAD ENZIMATICA

Row
34
Row
34

5
Producto Prod max Prod max
Tiempo X (g/L) S (g/L) rx
(g/L) biomasa P producto
0 0.378579481 24.11333521 5.85
1 0.395114619 21.85790809 7.20 0.395114619 0.017
2 0.519879744 23.63405695 8.55 0.259939872 0.125
3 0.453739196 24.11333521 9.00 0.151246399 -0.066
5 1.304847802 19.99718072 10.80 0.26096956 0.426
6 1.631792559 18.67211728 10.80 0.271965427 1.8 0.327
7 1.997068771 18.98223851 12.60 0.285295539 0.365
8 2.603156708 18.33380321 13.95 0.325394589 0.606
9 3.072904923 17.65717508 13.95 0.34143388 0.470
10 3.516347238 15.96560474 17.10 0.351634724 0.443
23 6.431416761 5.280518748 26.10 0.279626816 2.915
26 6.807215333 3.899069636 26.10 0.261815974 0.376

Ln
3 0.453739196 -0.7902327
5 1.304847802 0.266086407
6 1.631792559 0.48967914
rs rp rx especifico rp especifico
um G

-2.255 1.35 0.041848963 3.416730074


0.888 1.35 0.239988434 2.596754373
0.160 0.45 -0.14576777 0.991759152
-2.058 0.9 0.326133287 0.689735614
-0.221 0 0.200359265 0
0.044 1.8 0.182906176 0.901320989 0.4411 1.57140599
-0.081 1.35 0.232828064 0.518601126
-0.075 0 0.1528678 0
-0.169 3.15 0.126108796 0.895815967
-0.465 0.692307692 0.453254645 0.107644663
-0.053 0 0.055205918 0

0.6
f(x) = 0.4411404636x - 2.07
R² = 0.9716440778
0.4

0.2

0
2.5 3 3.5 4 4.5
-0.2

-0.4

-0.6

-0.8

-1
f(x) = 0.4411404636x - 2.0701445494
R² = 0.9716440778

2.5 3 3.5 4 4.5 5 5.5 6 6.5 Column C


Linear
(Column C)

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