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Syllabus For Biotechnology (BT) : Engineering Mathematics

The syllabus outlines topics in engineering mathematics, biotechnology, and bioinformatics that are covered in a Biotechnology course. Key topics include linear algebra, calculus, differential equations, probability and statistics, numerical methods, microbiology, biochemistry, molecular biology and genetics, process biotechnology, bioprocess engineering, plant and animal biotechnology, immunology, recombinant DNA technology, and major bioinformatics resources and techniques.

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Manoj Sk
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98 views2 pages

Syllabus For Biotechnology (BT) : Engineering Mathematics

The syllabus outlines topics in engineering mathematics, biotechnology, and bioinformatics that are covered in a Biotechnology course. Key topics include linear algebra, calculus, differential equations, probability and statistics, numerical methods, microbiology, biochemistry, molecular biology and genetics, process biotechnology, bioprocess engineering, plant and animal biotechnology, immunology, recombinant DNA technology, and major bioinformatics resources and techniques.

Uploaded by

Manoj Sk
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Syllabus for Biotechnology (BT)

ENGINEERING MATHEMATICS

Linear Algebra: Matrices and determinants, Systems of linear equations, Eigen values and
Eigen vectors.

Calculus: Limit, continuity and differentiability, Partial derivatives, Maxima and minima,
Sequences and series, Test for convergence, Fourier Series.

Differential Equations: Linear and nonlinear first order ODEs, higher order ODEs with
constant coefficients, Cauchy’s and Euler’s equations,Laplace transforms, PDE- Laplace, heat
and wave equations.

Probability and Statistics: Mean, median, mode and standard deviation, Random variables,
Poisson, normal and binomial distributions, Correlation and regression analysis.

Numerical Methods: Solution of linear and nonlinear algebraic equations, Integration of


trapezoidal and Simpson’s rule, Single and multistep methods for differential equations.

BIOTECHNOLOGY

Microbiology: Prokaryotic and eukaryotic cell structure; Microbial nutrition, growth and
control; Microbial metabolism (aerobic and anaerobic respiration, photosynthesis); Nitrogen
fixation; Chemical basis of mutations and mutagens; Microbial genetics (plasmids,
transformation, transduction, conjugation); Microbial diversity and characteristic features;
Viruses.

Biochemistry: Biomolecules and their conformation; Weak inter-molecular interactions in


biomacromolecules; Chemical and functional nature of enzymes; Kinetics of single substrate and
bi-substrate enzyme catalyzed reactions; Bioenergetics; Metabolism (Glycolysis, TCA and
Oxidative phosphorylation); Membrane transport and pumps; Cell cycle and cell growth control;
Cell signaling and signal transduction.

Molecular Biology and Genetics: Molecular structure of genes and chromosomes; DNA
replication and control; Transcription and its control; Translational processes; Regulatory
controls in prokaryotes and eukaryotes; Mendelian inheritance; Gene interaction;
Complementation; Linkage, recombination and chromosome mapping; Extrachromosomal
inheritance; Chromosomal variation; Population genetics; Transposable elements, Molecular
basis of genetic diseases and applications.

Process Biotechnology: Bioprocess technology for the production of cell biomass and
primary/secondary metabolites, such as baker’s yeast, ethanol, citric acid, amino acids, exo-
polysacharides, antibiotics and pigments etc.; Microbial production, purification and bioprocess
application(s) of industrial enzymes; Production and purification of recombinant proteins on a
large scale; Chromatographic and membrane based bioseparation methods; Immobilization of
enzymes and cells and their application for bioconversion processes.

Aerobic and anaerobic biological processes for stabilization of solid / liquid wastes;
Bioremediation.

Bioprocess Engineering: Kinetics of microbial growth, substrate utilization and product


formation; Simple structured models; Sterilization of air and media; Batch, fed-batch and
continuous processes; Aeration and agitation; Mass transfer in bioreactors; Rheology of
fermentation fluids; Scale-up concepts; Design of fermentation media; Various types of
microbial and enzyme reactors; Instrumentation in bioreactors.

Plant and Animal Biotechnology: Special features and organization of plant cells; Totipotency;
Regeneration of plants; Plant products of industrial importance; Biochemistry of major metabolic
pathways and products; Autotrophic and heterotrophic growth; Plant growth regulators and
elicitors; Cell suspension culture development: methodology, kinetics of growth and production
formation, nutrient optimization; Production of secondary metabolites by plant suspension
cultures; Hairy root cultures and their cultivation. Techniques in raising transgencies.

Characteristics of animal cells: Metabolism, regulation and nutritional requirements for mass
cultivation of animal cell cultures; Kinetics of cell growth and product formation and effect of
shear force; Product and substrate transport; Micro & macro-carrier culture; Hybridoma
technology; Live stock improvement; Cloning in animals; Genetic engineering in animal cell
culture; Animal cell preservation.

Immunology: The origin of immunology; Inherent immunity; Humoral and cell mediated
immunity; Primary and secondary lymphoid organ; Antigen; B and T cells and Macrophages;
Major histocompatibility complex (MHC); Antigen processing and presentation; Synthesis of
antibody and secretion; Molecular basis of antibody diversity; Polyclonal and monoclonal
antibody; Complement; Antigen-antibody reaction; Regulation of immune response; Immune
tolerance; Hyper sensitivity; Autoimmunity; Graft versus host reaction.

Recombinant DNA Technology: Restriction and modification enzymes; Vectors: plasmid,


bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; cDNA
and genomic DNA library; Gene isolation; Gene cloning; Expression of cloned gene;
Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain reactions;
DNA fingerprinting; Southern and northern blotting; In-situ hybridization; RAPD; RFLP; Site-
directed mutagenesis; Gene transfer technologies; Gene therapy.

Bioinformatics: Major bioinformatics resources (NCBI, EBI, ExPASy); Sequence and structure
databases; Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence
alignment, phylogeny); Genomics and Proteomics (Large scale genome sequencing strategies;
Comparative genomics; Understanding DNA microarrays and protein arrays); Molecular
modeling and simulations (basic concepts including concept of force fields).

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