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Oko Et Al 2019, Figure 8

Lytic replication is characterized by heterogeneous TMER localization and variable penetrance of actin RNA degradation.

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0% found this document useful (0 votes)

56 views1 page

Oko Et Al 2019, Figure 8

Lytic replication is characterized by heterogeneous TMER localization and variable penetrance of actin RNA degradation.

Uploaded by

Copyright

© © All Rights Reserved

We take content rights seriously. If you suspect this is your content, claim it here.

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A.

Gene 73
Gene 73
100
B.

mRNA
mRNA

TMER
TMER

DAPI
DAPI

Actin
Expt 1

RCA
Actin
RCA
Nuclear TMER
80

BF
Expt 2

BF
% Cells with

60
40
20
0
TMER: + + +
Gene 73: - + +
RCA: - - + Nuclear TMERs Cytoplasmic TMERs

C.
Mock Infected γHV68 Infected

TMER-/73- TMER-/73- TMER+/73- TMER-/73+ TMERlow/73low TMERhigh/73high

Input #: 202,669 Input #: 128,028 Input #: 29,096 Input #: 5,610 Input #: 8,956 Input #: 35,850

2500

1875
TMERs

1250

625

0
Gene 73 mRNA

4000

3000

2000

1000

250
RCA protein

187.5

125

62.5

5000
Actin mRNA

3750

2500

1250

D. TMERlow/ TMERhigh/
TMER-/73- TMER+73- TMER-/73+ 73low 73high

Figure 7
Oko et al

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