AU5800 Chemistry Analyzer

In-Lab Training Manual

___________________________________

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 AU5800 Chemistry Analyzer

WARNINGS AND PRECAUTIONS

Read all product manuals and consult with Beckman Coulter-trained personnel before
attempting to operate the instrument.

Beckman Coulter, Inc. urges its customers and employees to comply with all national
health and safety standards such as the use of barrier protection. This may include,
but is not limited to, protective eyewear, gloves, suitable laboratory attire when
operating or maintaining this or any other automated laboratory equipment.

INTENTION FOR USE

This document is not intended to replace the information in your User’s Guide or
Quick Response Guide. Information in the User’s Guide and Quick Response
Guide supersedes information in any other manual.

REVISION STATUS

Version 1.0 (May 2016)

Based on:
 AU5800 Chemistry Analyzer Software version 4.3
 AU5800 Chemistry Analyzer User’s Guide A98352AB
 AU5800 Chemistry Analyzer Quick Response Guide B00418AB

TRADEMARKS

AU5800 Chemistry Analyzer

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 AU5800 Chemistry Analyzer
In-Lab Training Manual

Table of Contents

Chapter 1 Analyzer Overview ....................................................................................... 7 

AU5800 Hardware ....................................................................................................... 8 
Sample Processing Overview .................................................................................... 13 
ISE Testing Sequence ............................................................................................... 17 
Chapter 2 Software Overview ..................................................................................... 19 
AU5800 Home Menu ................................................................................................. 20 
Main Button Area ....................................................................................................... 21 
Home Menu Area ....................................................................................................... 22 
Alarm Area ................................................................................................................. 23 
Menu List ................................................................................................................... 24 
Software Menu Path .................................................................................................. 25 
Online Help ................................................................................................................ 26 
Chapter 3 Daily Start Up ............................................................................................. 27 
Set the Start Conditions ............................................................................................. 28 
Daily Analyzer Maintenance....................................................................................... 30 
Check the Analyzer Status ......................................................................................... 34 
Check and Replenish Reagents................................................................................. 37 
ISE Start Up – Check the ISE Reagents .................................................................... 40 
ISE Start Up – ISE Daily Maintenance ....................................................................... 42 
Analyzer Calibration ................................................................................................... 46 
Process Quality Control (QC)..................................................................................... 48 
Chapter 4 Sample Processing and Programming .................................................... 51 
Sample Racks ............................................................................................................ 53 
Sample Data Prefix .................................................................................................... 57 
AU5800 Validated Sample Containers ....................................................................... 58 
Sample Processing .................................................................................................... 60 
Manual Programming ................................................................................................. 64 
Additional Sample Programming Features................................................................. 66 

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Chapter 5 Monitor and Review Results ..................................................................... 69 
Print RB/CAL/QC Results .......................................................................................... 70 
Calibration Monitor ..................................................................................................... 72 
QC Monitor and QC Data Review .............................................................................. 74 
Review Patient Results .............................................................................................. 78 
Chapter 6 Maintenance ............................................................................................... 81 
Maintenance Resources ............................................................................................ 82 
Weekly and Every Other Week Maintenance............................................................. 83 
Monthly Maintenance ................................................................................................. 93 
Chapter 7 As-Needed Tasks ..................................................................................... 101 
Fix (Assign) a Reagent Position ............................................................................... 102 
Set Calibrator Lot Concentrations ............................................................................ 104 
Configure New QC Lot ............................................................................................. 106 
Define a User Menu ................................................................................................. 108 
Save or Load System Parameters ........................................................................... 110 
Save Data to External Media ................................................................................... 112 
Perform an End Process (System Shutdown) .......................................................... 114 
Perform and Recover from an Emergency Stop ...................................................... 118 
Disable a Test .......................................................................................................... 120 
Transfer to Host ....................................................................................................... 122 
Chapter 8 Troubleshooting ...................................................................................... 124 
Alarms ...................................................................................................................... 125 
Flags ........................................................................................................................ 127 
Troubleshooting Reagent Blank, Calibration, and QC Failures ................................ 128 
Troubleshooting Tips ............................................................................................... 132 
Chapter 9 Resources ................................................................................................ 137 
Beckman Coulter Support Personnel ....................................................................... 137 
References............................................................................................................... 138 
Chapter 10 In-Lab Training Competency Exercise ................................................. 139 
In-Lab Training Competency Exercise Reference Key ............................................ 151 

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 Chapter 1
Analyzer Overview

Introduction

The AU5800 is an automated chemistry analyzer that measures analytes in samples, in

combination with appropriate reagents, calibrators, quality control (QC) material, and
other accessories. The system is for in vitro diagnostic use only. The AU5800 performs
automated analysis of serum, urine, and other fluids.

This chapter will cover the following:

 Analyzer and ISE hardware components
 Photometric sample processing overview
 ISE sample processing overview

Understanding the function of hardware components in the processing of samples on

the analyzer can assist in efficiently operating the system, as well as with performing
maintenance and troubleshooting.

Note: The ISE module is an optional module of the analyzer. You can skip any ISE-
related information and procedures in the In-Lab Training Manual if your lab does not
have the ISE module.

Practice

 Use the information presented in the following pages to identify the hardware
components on the analyzer

 Review the sample processing overviews for tests processed in cuvettes

(photometric) and the ISE unit

 Animations for the reaction process can be found on the Beckman Coulter website.
Use the following pathway to access the e-learning module:

www.beckmancoulter.com > SUPPORT > Training & Education >

Diagnostics/Chemistry > AU5800 > select On Demand tab:
 AU5800 Overview E-learning: Provides a hardware, ISE, and software
overview of the AU5800 with reaction process animations

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AU5800 Rack Transfer Hardware

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AU5800 Rack Transfer Hardware

Component Function

1 Rack Set Unit Holds rack set trays. Loads racks onto system
Rack Set Tray
2 Each tray holds up to 20 racks
1 or 2
Vertical Rack Transfers racks between rack set unit, rack buffer, rack
3
Transfer Unit transfer unit and rack collection unit
Twenty three positions temporarily hold racks before moving
4 Rack Buffer Unit
them to the rack transfer unit for either the initial or repeat run
Priority Rack Set Two positions are available to load racks onto the system with
5
Unit a higher priority than the rack set unit
RACK SET/DIAG Use this button to load a rack or tray when racks are moving
6
Button on the rack set unit. Also used to start maintenance functions
Primary Sample Moves routine racks to sample aspiration position on each
7
Transport Lane* unit
Moves red racks, repeat racks and priority load racks to the
8 Bypass Lane*
sample aspiration position on each unit
Moves racks to rack collection unit when sample aspiration is
9 Return Lane*
complete or to the buffer unit when Auto Repeat is enabled
Moves racks between the Primary sample transport lane,
10 Lane Changer
Bypass lane and Return lane
11 Collection Lane Moves racks to a rack collection tray
Rack Collection
12 Holds rack collection trays 1 and 2
Unit
Rack Collection
13 Each tray holds up to 20 racks
Tray 1 or 2
*7, 8, and 9 are the parts of the rack transfer unit (three belts)

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AU5800 Rack Feeder Unit with Automation
When the AU5800 analyzer is part of an automation line, the left side of the Rack
Feeder Unit is connected to the AU5800 Rack Builder Module.

AU5800
Rack Builder
Module

Component Function
Tray used for front loading of sample racks. Racks manually
1 Rack Set Tray loaded in the rack set tray or priority rack are processed
before racks automatically loaded from the Rack Builder
Rack Collection Tray used for collection of sample racks loaded on the rack
2
Tray set tray and priority rack set unit
Rack Builder Delivers AU5800 automation racks from the Rack Builder
3
Carry-in Lane Module to the analyzer
Rack Builder Returns the AU5800 automation racks processed on the
4
Carry-out Lane analyzer to the Rack Builder Module
 Loads samples from the automation line into AU5800
automation racks for processing on the analyzer
Rack Builder  Unloads processed samples from the AU5800 automation
5
Module racks and returns them to the automation line
 AU5800 automation racks are white racks with light blue
inserts
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AU5800 Sample Processing Hardware

Component Function
Two sample probes per analytical unit aspirate sample (in
conjunction with sample syringes) from the sample container and
Sample Probes dispense the sample into a cuvette. S1 of each unit delivers to
1 the inner cuvette wheel of each unit and S2 of each unit delivers
(S1, S2 and ISE)
to the outer cuvette wheel. The ISE sample probe delivers to ISE
sample pots in cells 1 or 2 in the ISE unit
Cuvette Wheels Houses 408 glass cuvettes in each unit, where reactions occur
2 and absorbance readings are taken
(Inner and Outer)
R1/R2 Refrigerated Two refrigerated compartments that house R1 or R2 reagents.
3 There are 54 positions in each compartment
Compartments
Aspirates and dispenses reagent, in conjunction with the reagent
R1 Reagent Probes
4 syringe, into glass cuvettes located in the cuvette wheel. R1-1
(R1-1, R1-2) delivers to the inner wheel; R1-2 delivers to the outer wheel
Aspirates and dispenses reagent into glass cuvettes located in
R2 Reagent Probes
5 the cuvette wheel. R2-1 delivers to the inner wheel; R2-2 delivers
(R2-1, R2-2) to the outer wheel
Mixes the contents of the cuvette after R1 reagent and sample is
6 R1/S Mix Unit dispensed into the cuvette. Mix bars are spiral shaped
Mixes the cuvette contents after R2 reagent is dispensed. Mix
7 R2 Mix Unit bars are L-shaped
8 Wash Nozzle Unit Cleans, rinses and dries cuvettes before and after analysis
The light source of the photometry system that is used to
9 Lamp measure reactions
Ion Specific Electrode (ISE) unit where Sodium, Potassium and
10 ISE Unit Chloride are measured. May consist of 1 or 2 cells
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AU5800 Hardware (Continued)

Component Function
Tank Storage The main wash solution tank that supplies wash solution to the
1 wash solution tanks located on each analytical unit
(below Rack Feeder)
This area houses the DI water tank, wash solution concentrate
Tank Storage
2 and dilute wash solution used throughout the system to wash
(Analyzer Unit) cuvettes and mix bars
Work with R2 probes to aspirate and dispense specified volumes
3 R2 Syringes of R2 reagent. R2-1 delivers reagent to the inner wheel, R2-2
delivers reagent to the outer wheel
Work with R1 probes to aspirate and dispense specified volumes
4 R1 Syringes of R1 reagent. R1-1 delivers reagent to the inner wheel, R1-2
delivers reagent to the outer wheel
Works with sample probe to aspirate and dispense the specified
5 Sample Syringes sample volume (located on the rear of the instrument)
Rinses the inside of the sample probe between samples (located
6 Wash Syringes on the rear of the instrument)
 ON (green): Turns on the analyzer and computer
 EM STOP (orange): Emergency stop turns off all power to
the analyzer immediately. Ctrl-Alt-Delete is required to turn
7 System Buttons
off the computer
 RESET (white): Use following an EM STOP or power failure
before pressing the ON button

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Sample Processing Overview

Step Action

1 A sample rack is placed on the rack feeder unit by the operator.

2 The operator selects Start from the Main Button Area.

The rack is moved to the bar code reader where sample programming is
3
identified.
4 The rack is moved to the sample aspiration position.

An R1 reagent probe, working with an R1

reagent syringe, delivers R1 reagent
5 (concentrated) and diluent into a cuvette:
 R1-1 delivers to the inner wheel
 R1-2 delivers to the outer wheel

An R1 mix bar mixes the diluted reagent

6
in the cuvette.

7 The photometer starts taking readings.

A sample probe, working with a sample

syringe, aspirates and dispenses sample
8 into the cuvette in the cuvette wheel:
 S1 delivers to the inner wheel
 S2 delivers to the outer wheel

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 Step Action

A sample mix bar mixes the sample and

9 the reagent. The photometer continues to
take readings.

If required, an R2 reagent probe, working

with an R2 reagent syringe, delivers the
R2 reagent (concentrated) and diluent
10
into the cuvette:
 R2-1 delivers to the inner wheel
 R2-2 delivers to the outer wheel

11 An R2 mix bar mixes the reaction mixture

The photometer continues to take

12
reaction readings.

The cuvette is washed, rinsed and dried

by the wash nozzle probes using diluted
wash solution and DI water

13 Note: Each wash nozzle is a 3-way

nozzle used to clean the cuvettes:
Longest aspirates liquid
Middle dispenses liquid
Shortest aspirates overflow liquid
When the sample is no longer needed the rack is moved to a rack collection
14
tray.

Note: A priority sample may be processed using the Priority Rack Set Unit at any time
during routine sample processing. This allows the priority sample to be processed
before the samples loaded in the rack set unit.
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AU5800 ISE Module

Component Function
ISE MID Standard
1 Conditions the electrodes
Solution
ISE Buffer
2 Dilutes the sample in the sample pot
Solution
ISE Buffer Aspirates and dispenses ISE Buffer Solution into the sample
3
Syringe pot
MID Solution
4 Delivers ISE MID Standard Solution to the sample pot
Roller Pump
ISE Reference Read by the reference electrode and used as a reference
5
Solution point in relation to the three electrodes
ISE Sample Works with the ISE sample probe to aspirate and dispense a
6
Syringe specific volume of sample into the sample pot
ISE Wash
7 Rinses the inside of the ISE sample probe between samples
Syringe

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ISE Testing Sequence

Step Action
The mixture aspiration roller pump pulls ISE Reference Solution from the bottle
1 and past the REF electrode where measurements are taken and the solution is
sent to waste.
2 ISE Buffer Solution is delivered to the sample pot by the ISE Buffer syringe.
Sample is aspirated by the sample probe and dispensed into the sample pot
3
where the sample and ISE Buffer Solution are mixed by the mix bar.
The mixture aspiration roller pump draws the diluted sample through the flowcell
where measurements are taken. Excess diluted sample in the sample pot is
4
pulled through the bypass tubing by the mixture aspiration roller pump and sent
to waste.
After a urine sample is processed, ISE Buffer Solution is delivered to the sample
5 pot by the ISE Buffer syringe and pulled through the flowcell by the mixture
aspiration roller pump to rinse the flowcell.
After each sample is processed, the MID Standard roller pump pulls ISE MID
Standard Solution from the bottle and delivers it to the sample pot. The mixture
6 aspiration roller pump draws the ISE MID Standard Solution through the flowcell
to condition the electrodes and take measurements and the solution is sent to
waste.
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 Chapter 2
Software Overview

Introduction

The software is used by the operator to interact with the analyzer and perform system
operations.

This chapter will cover the following:

 Common software screen areas
 Home menu of the AU5800
 Software screen structure
 Accessing the Help menu

Being familiar with the software can assist in understanding and efficiently operating the
system. Three software navigation methods are available for interacting with the
software:
 Touch screen
 Mouse
 Keyboard

Practice

 Use the information on the following pages to identify the common software areas
and the Home menu

 Access the Help button to identify the information available on the system

 The Main Button area buttons are not labeled on the software. Place the pointer of
the mouse over a button to identify the name of the button

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 AU5800 Home Menu
Main Button Area

Home Menu
Buttons

Shortcut Message
Buttons Area

Alarm Alarm Alarm

Message Clear List
Area

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 Main Button Area
The Main Button Area is viewable from all software screens.

Button Description

Home Displays the Home screen

Menu
Displays the Menu List screen
List

User Displays the User Menu screen (operator-defined shortcut

Menu buttons)
Mode
Displays the current mode. Displays the time remaining for
Display
certain maintenance operations
area

Start Starts analysis

Pauses analysis. The system pauses at the first test for which
Pause
no reagent was dispensed

Feeder Stops the rack feeder unit. The analysis of samples in racks
Stop that are loaded continues

Stop / Stops analysis. All data in process will be lost. In Stop mode,
Standby select this button to return the system to Standby mode

The system displays a menu for accessing the operator

Help
documentation and maintenance video directory

Logout Logs out an operator

Shuts down the system (End Process). Shutting down the

End system turns off the auxiliary power supply, including the lamp
and computer
Time
Display Displays the current date and time
area
.

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 Home Menu Area

Home
Description
Menu Button
Sample Status: Displays the sample status under analysis,
estimated time of completion, and results

Analyzer Status: Displays the analyzer status and temperatures

Message Area Description

 Displays messages regarding system conditions that
can affect analysis results

 Colors indicate the severity level of the message:

You cannot start analysis on the unit(s)

Red (ISE/analyzer) that generate the red
messages until they are addressed
You can start analysis. Review the message
Orange
carefully and take corrective actions
You can start analysis. Review the message
carefully and take corrective actions. Yellow
Yellow
messages will shift to orange (more severe)
if they are not addressed
A notification of system status. The system
Green
has no operational problems

 A symbol displays in the upper left of the screen

according to the most severe message color

 When a message is selected from the message area:

 The system displays a dialog with information and
corrective actions for the message. Select OK in the
dialog to go to the software screen to perform the
required action
 Highlights the affected area on the analyzer diagram

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 Home Screen Shortcut (Jump) Buttons
Shortcut (jump) buttons provide direct menu access to the most frequently used menus
to simplify software navigation.

Button Description

Set a new data index, the group of tests in use, the operator
name, and start sample numbers

Displays the R1 and R2 reagent status, and cleaning solution

status for the ISE and analyzer units

Displays the analyzer and ISE maintenance schedules, and use

to perform maintenance procedures

Displays sample information and manually order (requisition)

tests for patient samples, calibration, and QC
View and print reagent blank, calibration, QC, and patient data,
and batch transfer data to the laboratory information system
(LIS)

Alarm Area
Displays alarm messages generated during system operation.

Button Description

Select to stop the audible alarm. Select a second time to

Alarm Clear
clear the alarm message from the screen

Select to display the Alarm List. The system can store

Alarm List
and display a maximum of 4096 alarms

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 Menu List

This column displays the software menus. This column displays the submenus
The selected menu is highlighted in blue. for the selected menu.

Menu Description
Perform various basic operational procedures: set start conditions,
Routine manage reagents, order samples/calibrations/QC, view/print results,
review reaction data
View history of calibration information and perform calibration
Calibration verification

QC View and manage QC data and charts

Program information for all tests, including calibration and QC

Parameters parameters

Monitor and perform maintenance, review a detailed alarm log, and

Maintenance perform diagnostic functions

Program online conditions, list formats, comments, bar code options,

System system settings, and data management

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 Software Menu Path

1 Menu List
2 Menus
3 Submenus
4 Screens
5 Tabs
6 Buttons for corresponding function keys (F1 – F8 on the keyboard)

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 Online Help

 Select the Help button from the Main Button area to access the AU5800 Chemistry
System Online Help Main Menu

 Online Help includes

 PDF versions of User’s Guide and Quick Response Guide
 PDF versions of additional documentation (Automation Addendum)
 Video Directory with videos of select maintenance procedures

 Tips for using Online Help:

 PDF documents can be printed – be sure to enter a page range before printing if
only printing a specific section or procedure
 Blue text within the PDF document can be selected as a link to other procedures
 The Online Help is screen-specific. Selecting Help automatically takes you to the
section of the User’s Guide that relates to the current screen

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 Chapter 3
Daily Start Up

Introduction

A daily start up should be performed daily on the AU5800 prior to processing patient
samples. The following procedures are required for a daily start up:

 Set the start conditions

 Perform daily analyzer maintenance
 Check the analyzer status
 Check and replenish reagents
 Perform an ISE start up
 Perform analyzer calibration
 Process quality control (QC)

These procedures are presented in this chapter in an efficient order. However, you may
identify an order that may be more efficient based on your lab policies and processes.

Flowchart Information

Flowcharts are provided for each daily start up procedure in this chapter. These
flowcharts are available as a quick reference for the daily start up procedures.

Review the User’s Guide for Warnings, Cautions, Important, Note, and Tip alerts as well
as step-by-step instructions. For complete instructions to the daily start up procedures,
refer to:

Reference: AU5800 Chemistry Analyzer User’s Guide

 Chapter 5: Daily Start Up

Practice

 Use the information and flowcharts on the following pages to practice the daily start
up procedures and determine an efficient order based on your laboratory workflow

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Set the Start Conditions

The Start Condition menu is used to create a data index, select a group of tests, and
enter the operator name.

An index is a data file identified by the date and time. Create a new index daily, each
shift, or as needed. There are two options available when creating a new index:
 Option 1: Create a new index from the New Index window that displays when
the analyzer is turned on from an End Process (system shutdown). This option is
available only for labs that shut down the analyzer at the end of the day
 Option 2: Create a new index from the Start Condition menu. This option is
available for continuously operating labs or if there is a need to create another
index in the same day

The group of tests determines the onboard tests to be performed in the index. Up to
three different groups can be defined on the system. A group of tests can be changed
without changing the index.

Practice

 Use the flowchart on the next page to practice creating a new index. To practice
creating an index following option 1 (turning the system on from an End Process),
you will need to first shut down and then turn the analyzer back on. Refer to
Chapter 7 As-Needed Tasks  Perform an End Process (System Shutdown) of this
manual for instructions on performing an end process

 Determine if your lab will have multiple “Group of Tests” defined. If so, identify when
you would need to change the group of tests

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 Set the Start Conditions

System
Yes No
powered on from an
End Process?

Select New Index option

Select Home
from the New Index window

Select “Group of Tests”, if necessary Select Start Condition jump button

Select OK to create a new index Select Edit F1

Select New Index button

Set the following conditions, if

necessary:

“Group of Tests”
“Operator Name”
“Start Sample No.”

Select Confirm F1

Select OK to create a new index

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Daily Analyzer Maintenance

Maintenance should be performed on the analyzer to ensure system performance and

safety. Daily analyzer maintenance involves a series of inspections and the
replenishment of solutions.

A Maintenance Schedule List is available for you to document the completion of

maintenance procedures on the AU5800.

Note: The daily maintenance procedure to “Inspect, Clean, and Prime the ISE Probe” is
included in this section with the analyzer probes instead of the ISE Start Up – ISE Daily
Maintenance section of this chapter.

For a copy of the Maintenance Schedule List, refer to:

Reference: AU5800 Chemistry Analyzer User’s Guide

 Chapter 8: Maintenance
 8.1 Using the Routine Maintenance Schedule
 8.1.1 Maintenance Schedule List

Practice

 Use the flowcharts on the following pages to practice the daily maintenance
procedures. Procedures are presented in an efficient order in the flowchart but do
not need to be performed in the order listed

 Identify where to find spare parts in the lab for components that require replacement
following inspections: syringes, mix bars, probes

 Identify the solutions that need to be replenished for the bottle positions on the
outside of the reagent refrigerators and sample probes:
 Diluent
 Det.-1, Det.-2
 CLN-1, CLN-2 (lab dependent contamination parameters; check with your
Applications Specialist for the appropriate solutions)

 Based on your laboratory workflow, which shift will be in charge of daily

maintenance?

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 Daily Analyzer Maintenance

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 Daily Analyzer Maintenance, continued

Daily Maintenance procedures continued from the previous page

Inspect the Printer and Paper

Verify the printer is on

Verify that there is enough paper in the printer

Inspect the Stability of the Upper Cover

Verify that the upper cover for each analyzer unit is stable and remains in the
upright position when raised

Replace the DI Water in the Pre-dilution Bottles

Discard the water in the pre-dilution bottles located outside of the R1 reagent
compartment in positions labeled 55. Diluent/W2 and 56. Diluent/W2
Rinse the bottles twice with DI water
Fill the bottles with DI water and replace it on analyzer unit
Repeat for each analyzer unit

Prepare the Sample Probe Wash Solutions

Verify there is approximately 50 mL of 2% wash solution in the two 60 mL

bottles located by the sample probes for each analyzer unit and the (Optional)
ISE unit. The positions are labeled 61. Det.-1/W2, 62. Det.-2 and 63. Det.-1/W2,
64. Det.-2 for the analyzer sample probes and Det.-1/W2, Det.-2 for the ISE
sample probe
Note: Heavy contamination may require 50 mL of a 1:5 dilution of Beckman
Coulter Sodium Hypochlorite (5%) in the Det.-2 positions.

Contamination Parameters

If you have contamination parameters defined on your system, verify you have
sufficient volume of the appropriate cleaning solution in each bottle
(60 mL bottles located by the reagent probes in positions labeled
55. CLN-1, 56. CLN-2, 57. CLN-1, and 58. CLN-2 for R2 reagent probes and
57. CLN-1, 58. CLN-2, 59. CLN-1, and 60. CLN-2 for R1 reagent probes)

Verify the Handle on the Diluted Wash Solution Tank

is in the Open Position

Open the right door of each analyzer unit

Verify the handle on the diluted wash solution tank is in the Open position
If necessary, turn the handle to the Open position
Close the door to each analyzer unit

Document completion of Daily Maintenance tasks

on the paper Analyzer Maintenance Schedule List

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Check the Analyzer Status

The Analyzer Status menu displays a color-coded overview of the system and the
temperatures of the incubator and refrigerated R1/R2 compartments. The following
colors indicate the status of the components:
 Blue: No errors
 Yellow: Non-fatal error. The unit can be started
 Red. Fatal error. The unit cannot be started

The rack set trays, rack collection trays, rack transport belts, wash solution tanks, DI
water tanks, waste tanks, printer, and LIS communication are also monitored. The ISE
unit and reagents are monitored when the ISE unit is installed.

Notes:
 Analyzer Status also displays the location of in-process racks on the rack feeder unit
and the rack transfer unit (primary sample transport, bypass, and return lanes)
 Select Rack Data F1 from Analyzer Status to view the time a rack was detected on
the rack feeder unit (Pre-Analysis) and the time the rack moved to the rack collection
area (Post-Analysis)

For a description of the display color status for each component, refer to:

Reference: AU5800 Chemistry Analyzer User’s Guide

 Chapter 7: System Monitoring and Results
 7.2 Monitoring Analysis
 7.2.4 Analyzer Status Menu

Practice

 Use the flowchart on the following page to review the Analyzer Status menu.
To cause the display color to change for different components, you can:
 Open the covers of the refrigerators, ISE unit, or rack buffer
 Remove an ISE reagent from its tray

 Locate the Bath Temp and Coolant Temp displays. Determine if your lab requires
daily documentation of the temperature readings on a separate log

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 Check the Analyzer Status

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Check and Replenish Reagents

A reagent check should be performed during a daily start up to determine the number of
tests onboard and to update the status of the reagents and cleaning solutions.

Reagent volume and status can be viewed from tabs in Reagent Management:
 Main tab: Provides volume and status of reagents by sample type. The display
color for each reagent indicates the following for the selected analyzer unit:
 Orange: Reagent is missing, expired, or empty
 Yellow: Reagent volume is short (low)
 Light Blue: Required reagents are set (present)
 Green: Reagents do not meet volume requirements set in Reagent Inventory
 Gray: The reagent is not used for the displayed sample type
 Details tab: Provides detailed information for each onboard reagent in the
selected analyzer unit. Use the Shots, Onboard Remaining, and Expiration
columns to identify reagents that need to be replaced

Reagent Check: The analyzer requires a reagent check any time the reagent
refrigerator covers are opened or when selecting the Edit function in the Parameters
menu. The following options are available when performing a reagent check:
 Check all positions: Checks the remaining volume of reagents at all positions,
including the bottle positions outside of the R1/R2 compartments and sample
probes
 Check specified positions: Checks the remaining volume of reagents at
specified positions selected by the operator
 Check changed positions: Checks the remaining reagent for any reagent ID
(bar code) that is new or has been moved since the previous reagent check
 Reset Only: The reagent volume check and bar code reading is not performed.
Select this option when a reagent cover is opened without changing any reagent,
or a Parameters screen is entered, but no changes are made
These checkboxes can be selected/deselected in the Reagent Check dialog
 Unit: The Unit checkboxes are selected as the default. A reagent check will not
be performed for analyzer units that are deselected
 ISE: The ISE checkbox is selected as the default to perform a volume check of
the ISE detergent bottles located by the ISE sample probe:
 The system performs only an ISE detergent volume check when all Unit
checkboxes are deselected in the Reagent Check dialog
 The ISE checkbox is automatically deselected when a reagent check is
performed while the ISE unit is busy

Note: A reagent check resets any manual calibration or QC orders back to

automatic order requirements (based on calibration status and default QC profile).

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Check and Replenish Reagents, continued

Practice

 Use the flowchart on the following page to practice loading reagents and performing
a reagent check

 Review the Details tab to identify the additional information that displays for onboard
reagents

 Based on your test volume, at what number of shots (tests) will you decide to
replace or add reagent bottles?

 Perform each reagent check option to identify the differences between them
Note: The Checked changed positions option will require the moving or addition
of a bar coded reagent to see the reagent probes perform a volume check.

 Perform a ISE detergent check only by deselecting the Unit checkbox(es)

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 Check and Replenish Reagents

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ISE Start Up – Check the ISE Reagents

Each flowcell in the ISE unit uses three reagents:

 ISE Buffer Solution
 ISE MID Standard Solution
 ISE Reference Solution

Check the ISE reagents daily for onboard stability and volume:
 All three ISE reagents have a 90-day onboard stability that needs to be tracked
by the operator. The analyzer does not track ISE reagent onboard stability. It is
recommended to write the onboard expiration on the bottle when loading an ISE
reagent
 The system generates an “ISE Reagent Short” alarm when the reagent volume is
low (5.2 cm above the bottom of the bottle). The approximate number of
samples that can be processed for each reagent when the alarm is generated is:
 Buffer: 240 samples
 MID Standard: 180 samples
 Reference: 600 samples
 For systems with two flowcells, if one flowcell runs out of reagent, processing
continues with the flowcell that has sufficient reagent

When loading ISE reagents:

 ISE reagents cannot be loaded and primed when the ISE unit is in Measure
mode. Verify there is sufficient reagent volume on board before starting analysis
 ISE reagents can be loaded and primed, and ISE calibration can be performed,
when the analyzer is in a Measure mode, as long as the ISE unit is in a Ready
mode. Confirm the ISE unit displays Ready each flowcell (Cell 1 and Cell 2) from
the Analyzer Status screen
 DO NOT mix old and new reagent
 It is recommended to perform calibration when a new bottle is loaded even if the
24-hour ISE calibration period is not due

Practice

 Use the flowchart on the following page to practice loading an ISE reagent

 Determine the volume at which your lab will replace an ISE reagent:
 Based on your daily sample volume, can you wait until the alarm is generated?
 Will you have each shift check the volume prior to processing samples?

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 Load ISE Reagents

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ISE Start Up – ISE Daily Maintenance

ISE Daily Maintenance includes:

Note: The daily maintenance procedure to “Inspect, Clean, and Prime the ISE Probe” is
included in the Daily Analyzer Maintenance section of this chapter with the other
analyzer probes.

ISE Cleaning
 Perform daily to clean the ISE sample pot and electrode lines. Contamination or
inaccurate results may occur if the cleaning is not performed
 For labs that will perform an End Process (system shutdown) each day, it is
recommended to perform the cleaning just prior to shutting down the analyzer to
remove any build up before the ISE is not in use for a period of time
 Procedure takes approximately 6 minutes to complete

ISE Calibration
 Perform once every 24 hours, following specific maintenance procedures, and
when replacing ISE reagents
 If the ISE calibration is performed immediately after the ISE cleaning, perform a
total prime before calibrating
 The ISE Serum and Urine Standards have an open-bottle stability of 90 days
 Use the Slope tab to review the slope results in graphical format for consistency
across calibrations for each flowcell

Practice

 Use the flowcharts on the following pages to perform the ISE Daily Maintenance
procedures

 Determine when you will perform the ISE cleaning and ISE calibration based on your
laboratory operating hours

 Identify if your lab will calibrate serum only, urine only, or both

 Review the Slope chart

 The system automatically updates the ISE Cleaning and ISE Calibration procedures
when they are completed in the ISE Maintenance screen. Confirm that the system
has the correct “Performed” date and time after the procedures are complete

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 ISE Cleaning

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 ISE Calibration

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Analyzer Calibration

During a reagent check, the system automatically orders reagent blank (RB) and
calibration (CAL) for all tests with:
 Reagent blank or calibration expired
 Reagent blank or calibration expires soon*
 New bottle or lot number for the reagent
 Reagent blank or calibration failed

* Your Beckman Coulter Applications Specialist can define “expires soon” to a time
period that meets your laboratory workflow.

You can always edit the automatic order by adding or removing tests for reagent blank
and calibration.

The reagent blank and calibration status (RB/CAL Stability Remaining) for a test can be
found in the Reagent Management: Details tab.

Practice

 Use the flowchart on the following page to review the tests automatically
requisitioned for calibration and the calibrators required

 Identify the “expires soon” time set for your analyzer

 Identify where to see the RB Stability and CAL Stability Remaining times from the
Reagent Management: Details screen. The system displays remaining times in
days (D) and hours (H). Select the column name to sort the times in ascending
order (tests with the shortest RB and CAL Stability Remaining times sort to the top of
the column)

 Use the flowchart to practice manually ordering tests for calibration

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 Analyzer Calibration

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Process Quality Control (QC)

Quality control analysis is used to confirm system performance. QC should be

performed prior to processing patient samples, following calibration, following
maintenance procedures, and according to laboratory policy. Each laboratory should
establish its own control frequency.

QC can be processed on the system by:

 Using a default QC profile
 Manually ordering QC tests

When a default QC profile is defined on the system, all tests defined in the profile will be
automatically ordered when a reagent check is performed. The default QC profile is
usually setup by the Applications Specialist during installation.

The default QC profile is automatically ordered only for the first bottle (sequence 1) of
each reagent onboard the system. If multiple bottles of the same reagents are on the
system, you will need to manually order QC for the additional bottles (individual
requisition) if your lab policy requires QC to be processed on the additional bottles. Up
to 5 sequence bottles can be loaded on an analyzer unit for a reagent.

If a default QC profile is not defined, then the operator is required to manually order QC
each time it needs to be processed on the analyzer.

Note: The following flowcharts do not apply if your lab uses the LIS to generate QC
requests and monitor QC results.

Practice

 Use the flowcharts on the following pages to practice processing QC:

 Use the “Process Quality Control (QC) Using a Default QC Profile” flowchart if
you have a default QC profile defined on your system
 Use the “Manually Order Quality Control (QC)” flowchart if you do not have a
default QC profile defined

 Based on your test volume, will you require multiple bottles of reagents on the
analyzer? You can identify the multiple bottles in the “Seq.” column of the Reagent
Management: Details tab

 Use the “Manually Order Quality Control (QC)” flowchart when you need to rerun or
order QC for specific tests

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 Process Quality Control (QC) Using a Default QC Profile

Select Home
Select Rack Requisition jump button
Select QC button

Review default QC profile requisition:

Select sample type from the “Type” drop-down list

to view tests requisitioned for QC by the analyzer for each sample type

Note: The analyzer will automatically requisition all tests in the

default QC profile, if the default is defined on your system.
Tests highlighted in blue are requisitioned for QC.

QC
multiple bottles No
of the same
test?

Yes

Requisition QC for multiple bottles:

Select Start Entry F1

Select Individual Requisition F3 (for multiple analyzer unit
systems the default is Unit No. 1)
Select Select All to requisition all bottles for all tests
Select sample type from the “Type” drop-down list to requisition
controls for other sample types
(For multiple analyzer unit systems) select Unit No. to select tests
from specific analyzer units
Select Close
Select Entry F2 to save the requisition

Select Display Cup Set F5 to view the controls and racks required
Note: You may need to scroll down, or select Print, to view all
required controls
Select Close

Load the controls according to the list in the correct racks

and place the racks on the rack set unit

Select Start from the main button bar

Review errors on the Error List in the Start window and perform any
corrective actions, if necessary
Select Start from the Start window

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 Manually Order QC

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 Chapter 4
Sample Processing and Programming

Introduction

In this chapter you will identify the racks and validated sample containers that are
available on the AU5800. The racks and containers that you use in your lab are setup
by your Applications Specialist and Service Engineer during installation.

It is important to reduce the risk of system errors and to provide accurate test results by
using the appropriate racks and validated containers when processing samples on the
analyzer.

This chapter will also include information on:

 Sample data prefixes
 Processing samples with bar codes and LIS programming
 Processing and programming samples without LIS programming
 Ordering an add on or rerun test at the analyzer
 Batch programming samples on the analyzer

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Sample Racks

The system identifies the rack type from the combination of magnets set into the bottom
of the rack. The color of the rack is for the operator to easily identify the rack type.

The system identifies the sample type for the rack from the rack ID bar code label. The
rack ID bar code range for sample type is configured in the AU5800 software. The
following sample types are available on the AU5800: Serum, Urine, Other-1, Other-2.
Place samples in the correct rack for the sample type.

Note: Labs that use a Beckman Coulter automation system will have racks setup for
mixed sample types. There may also be exceptions for mixed sample types on racks
based on your specific laboratory requirements. Verify rack setup on your system with
your Applications Specialist.

Any validated container can be setup to be used for processing reagent blank,
calibrators, QC, or patient samples in the appropriate rack type. The available validated
containers for the AU5800 will be addressed later in this chapter.

Rack Adapters

Rack adapters (black) are necessary to hold smaller diameter tubes (11.5 to 13.5 mm)
firmly in position in the racks. Larger diameter tubes (13.6 to 16 mm) do not require
adapters.

Note: For labs connected to an automation line, white racks with non-removable light
blue adapters are used in the Rack Builder Module.

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Reagent Blank, Calibration, and QC Rack Types

When multi-rack ID is used, analysis for reagent blank, calibration, and QC takes place
simultaneously on multiple units for faster results. Each rack has a rack bar code which
specifies the unit number.

Example: If you have a system with 4 units and you have your blue racks setup for
multi-rack ID, then:
 Blue rack for Unit 1, rack ID = 1001
 Blue rack for Unit 2, rack ID = 2001
 Blue rack for Unit 3, rack ID = 3001
 Blue rack for Unit 4, rack ID = 4001

Multi-rack ID can be setup separately for Reagent Blank, Calibration, and QC racks.

Blue Rack

 Use to process reagent blanks

 Use any validated container to place

DI water in position 1

Yellow Rack
 Use to process calibrators

 Multiple racks may be required

depending on number of calibrators
and sample types used

 Racks may be configured for rack ID

and position or bar code operation
Green Rack
 Use to process QC material

 Multiple racks may be required

depending on number of controls and
sample types used

 Racks may be configured for rack ID

and position or bar code operation

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Patient Sample Rack Types

 Barcode analysis: Bar coded samples may be placed in any position in the rack
programmed for the correct sample type
 Sequential analysis: Samples without bar codes must be placed in numeric order
by their sample number order. Do not leave empty spaces between samples
Note: Barcode analysis is recommended because sequential analysis does not
allow for positive patient identification.

White Rack

 Use for routine patient samples

 Use for Automatic Repeat samples

(repeat is automatically ordered and
processed by the analyzer in the same
rack)

Orange Rack
 Use for Manual Repeat samples
(repeat is automatically ordered by the
analyzer, but only processed when
placed into an orange rack)

 Samples that require repeat are

identified from the Repeat Run Pending
List (Menu List > Routine >
Repeat Run > Pending List F4)
Red Rack

 Use for emergency (STAT priority)

patient samples

 Can also be used for Automatic Repeat

samples

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Sample Data Prefix

The system displays the sample data prefix in front of the sample number. The sample
data prefix can help you identify the following information about a sample processed on
the analyzer:
 If the sample data is for a reagent blank, calibration, QC, or patient sample
 If the sample was processed in a routine (white) rack, an emergency (red) rack, or a
manual repeat (orange) rack
 If the patient sample result is from the original run or the repeat run of the sample

Type Normal Run Repeat Run

Serum (None) H

Routine Urine U HU
Sample Other-1 X HX
Other-2 Y HY
Serum E HE

Emergency Urine UE HUE

Sample Other-1 XE HXE
Other-2 YE HYE
QC Q
CAL A
RB R

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AU5800 Validated Sample Containers
Note: BD indicates a Becton Dickinson part number. The BD tube or its equivalent can be
used.

Dead
Container
Description Part Number Volume Example
Type
(µL)

Serum Separator Tube

BD 367986
13x100 mm

Serum Separator Tube

BD 367988
16x100 mm

Lithium heparin with gel

separator (light green top) BD 367960 4 mm
13x75 mm above the
non-sample
Lithium heparin with gel (cells or gel)
separator (light green top) BD 367962 layer
Primary
13x100 mm
Tube
Lithium heparin (green top)
BD 367884
13x75 mm

Lithium heparin (green top)

BD 367886
13x100 mm

Primary tube (red top)

BD 366668
13x75
140
Primary tube (red top)
BD 367815
13x100

Aliquot Auto Aliquot Tube

2910034 90
Tube 13 mm

Sample Hitachi Cup

MU853200 70
Cup 2.0 mL

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AU5800 Validated Sample Containers

Cup Nested (Inserted) in Tube Dead

Tube Part Volume Example
Cup Description Part Number (µL)
Number
Access 2 Cup, 1.0 mL
13x75 mm
BD 367960
81915 140
BD 367884
BD 366668

Access 2 Cup, 2.0 mL 81902

DxC Cup, 2.0 mL 652730 DxC Transfer

Tube 70
979272

13x75 mm
EZ Nest Cup BD 367960
BD 367884
BD 366668
1270013000 50
13x100 mm
BD 367962
BD 367886
BD 367815
EZ Nest Cup
16x75 mm
BD 364976
1270016000 80
16x100 mm
BD 367988

Hitachi, 2.0 mL
SST
MU853200 16x100 mm 70
BD 367988

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Sample Processing

When processing samples on the analyzer:

 Determine if samples have sufficient volume to process in the primary tubes.
Transfer any samples with insufficient volume into validated low volume containers.
Use of non-validated containers may result in sampling errors
CAUTION: A field service engineer sets the sample probe descent and alignment
for optimal sampling during your system installation. Do not change the type of
containers used on a rack without consulting Beckman Coulter technical support.
 Remove all caps
 Barcode analysis: Samples can be placed in any position in the rack, but a bar code
is required. Bar code labels should be placed at least 7 mm from the bottom of the
container
 Place each sample in the correct rack for:
 Sample type: In Barcode analysis, if a sample is placed in the incorrect rack for
sample type, the system will generate an alarm and will not process the sample
 Tube size: Confirm a tube fits correctly in a rack by placing the tube into a rack
with and without an adapter and determine which holds the tube most securely
 Confirm the sample bar codes are aligned in the center of the open slots in the rack
 Confirm there is sufficient sample volume in insert cups (including dead volume) to
prevent sample probes from crashing into the bottom of the insert
 Load racks on the rack set unit with position 1 of the rack (rack ID bar code end)
facing to the right
 Use the priority rack set unit to process samples with higher priority
 The system will use the Bypass Lane to process samples loaded in red racks in
the priority rack set unit before racks loaded on the rack set unit

Practice

 Use the flowcharts on the following pages to process samples on the rack set unit or
the priority rack set unit. The flowcharts apply for systems setup for Barcode
analysis
Reminder: When connected to an automation line, front load racks in the priority
rack set tray and left rack set tray only. These racks will unload in the right tray.

 Based on your laboratory workflow, how will you manage priority samples? Will you
need to use Emergency (red) racks or the priority rack set unit?

 Identify if you will require racks for different sample types and tube sizes. How will
operators be able to differentiate racks for sample type?

 If you have an analyzer with multiple units, will your reagent blank, calibration,
and/or QC racks be setup for the multi-rack ID feature? Will they be setup for rack
ID and position or bar code operation?

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 Sample Processing using the Rack Set Unit

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 Sample Processing using the Priority Rack Set Unit

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Manual Programming

Samples can be manually programmed and processed on the AU5800 when the LIS is
down or if the sample does not have an LIS-generated request. Samples can be
manually programmed for processing on routine or emergency racks.

When the system is setup with racks for Barcode analysis, manually programmed
samples require a bar code. A roll of alternate bar codes is provided with your analyzer
during installation for you to use when manually programming. You may also have
“downtime” bar codes in your lab that you can use for manual programming.

When the system is setup with racks for Sequential analysis, you will need to manually
program all samples processed in the sequential racks, but they do not require a bar
code on the sample. Samples processed in sequential racks must be placed in the rack
and onto the analyzer in the order they are manually programmed on the system.
Note: Barcode analysis is recommended because sequential analysis does not allow
for positive patient identification.

To identify the Analysis mode for racks on your system, use the following pathway on
the software:
Menu List > System > System Condition > Analysis mode > [Test Requisition]

Practice

 Use the flowcharts in the following pages to practice manually programming samples

 Which bar codes will be used in your lab when a sample without a bar code is
manually programmed?

 Routine (white) and Emergency (red) racks can be set up separately for Barcode or
Sequential analysis. Will you have racks setup for sequential analysis?
 If so, how will you manage processing samples on these racks?
 How will this affect the processing of bar coded STAT samples on emergency
racks?

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 Manual Programming for Samples

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Additional Sample Programming Features

The Rack Requisition Sample screen provides additional features for manually
programming samples at the analyzer:
 Add on or rerun tests
 Batch programming

The system allows for manual ordering of add on and rerun tests for samples previously
processed in the same index. Use the Add On button when:
 The order is required to be manually ordered at the analyzer (not from data
management system or LIS)
 The order will be on samples that were processed in the current index
 The order requires the use of the original sample ID (bar code) from the first run
of the sample
 The order will be processed using the same Sample Kind (Routine rack,
Emergency rack) as the first run of the sample
If any of these conditions do not apply to the request, then the add on or rerun can be
manually ordered as a “new” sample.

Batch programming allows for the ordering of the same tests on a number of samples
without having to order each sample individually.

Practice

 Use the flowcharts on the following pages to practice ordering add on/rerun tests or
batch programming
Note: The add on/rerun procedure can only be performed on a sample that has
been processed in the current index.

 When will you need to manually order an add on or rerun test at the analyzer?

 When will you need to batch order at the analyzer?

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 Add On and Rerun Tests on Racks

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 Batch Order

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 Chapter 5
Monitor and Review Results

Introduction

It is important to review the results for reagent blank, calibration, and QC before
processing samples. Patient samples should be reviewed for any flags before releasing
results.

This chapter will cover the following:

 Reviewing calibration status from the Calibration Monitor
 Reviewing QC charts
 Managing QC data
 Reviewing patient results

The system can be setup to automatically print reagent blank, calibration, QC, and
patient results. You can also reprint reports when needed.

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Print RB/CAL/QC Results

Reagent blank, calibration, and QC results should be reviewed for flags. These results
can be reviewed from the printed reports or from the Detail or Realtime Display screen
in Sample Status.

The analyzer is usually set to automatically print reagent blank, calibration, and QC
results when they are complete. These reports can be reprinted from Sample Manager
when needed.

Practice

 Use the flowchart on the following page to print reagent blank, calibration, and QC
results from Sample Manager

 When might you need to reprint a reagent blank, calibration, or QC report?

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 Print RB/CAL/QC Results

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Calibration Monitor

Use the Calibration Monitor to:

 Review the reagent blank and calibration status for a test
 Review the reagent blank and calibration history for a test
 Review the reagent blank and calibration details for a test

The Status tab provides a color-coded status of reagent blank and calibration for each
test by sample type, cuvette wheel (inner or outer), and for analyzers with multiple units,
unit number. The display color indicates the following for RB and CAL:
 Orange: Bottles without calibration data, with failed calibration data, or with
expired calibration data exist
 Yellow: Bottles with calibration data that expires soon exist
 Light Blue: No errors (current calibration passed)
 Green: Status cannot be determined until a reagent check is performed
 White: Tests are not for the selected sample type, cuvette wheel, and unit

The RB History and Calibration History tabs provide a graph of the reagent blank and
calibration data for a test. The system saves a maximum of 100 points of data per
sample type per test. The graphs can be used to check for consistency of reagent
blank and calibration data.

The RB Detail and Calibration Detail tabs provide the detailed data for the selected
test.

Practice

 Use the flowchart on the following page to review the reagent blank and calibration
status, history, and details for tests from the Calibration Monitor

 Calibration status for a test can also be viewed from the Comment column of the
Reagent Management: Details tab. What is the benefit of using the Calibration
Monitor to view calibration status?

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 Calibration Monitor

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QC Monitor and QC Data Review

Use the QC Monitor to:

 Review QC data review status
 Review and print QC charts
 Review QC statistics

The QC Monitor displays the QC data review status for the QC levels processed for
each test in the current index. The color for each QC level processed for a test
indicates the status:
 Green: Normal data (results in range)
 Yellow: Data outside the Single Check Level programmed in QC Parameters
 Red: Abnormal data (not included in QC statistics)

Use QC Data Review to:

 Delete QC data
 Enter comments for QC results

If you are using the analyzer to generate QC statistics you may need to manage QC
data if it is processed incorrectly (levels switched, wrong QC used) to not include the
data in the statistics calculation.

Practice

 Use the flowcharts on the following pages to review and manage QC results

 Will you use the analyzer to review and manage QC results, or will this be done
using another QC program (on a data management system or LIS)?

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 Review and Print QC Daily Chart

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 Delete QC Data and Enter Comments

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Review Patient Results

Patient results can be reviewed from the software or on the printed reports.

In progress and completed samples can be viewed from the Sample Status screen of
the Home menu. Completed samples can be reviewed and printed from the Sample
Manager screen.

Patient results should be reviewed for flags. Error flags are generated by the system
when it encounters a condition that can affect the result. This condition can range from
minor warnings to severe errors that require immediate attention. No result should be
reported with an unresolved or unexpected flag. When in doubt, always consider
repeating the sample analysis.

Flags are displayed to the right of the patient result. They are displayed in red when
results are reviewed on the Sample Status screen.

Practice

 Use the flowcharts on the following pages to review patient results on the software
and print patient results

 Will patient results be reviewed from printed reports or at the analyzer? Or will they
be reviewed on a data management system or at the LIS?

 When might you need to print a patient report? Review the different report formats
available on your system for printing patient reports

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 Review Patient Results from Sample Status

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 Review and Print Patient Results from Sample Manager

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 Chapter 6
Maintenance

Introduction

Maintenance should be performed on the analyzer to ensure system performance and

safety.

A Maintenance Schedule List is available for you to document the completion of

maintenance procedures on the AU5800.

For a copy of the Maintenance Schedule List, refer to:

Reference: AU5800 Chemistry Analyzer User’s Guide

 Chapter 8: Maintenance
 8.1 Using the Routine Maintenance Schedule
 8.1.1 Maintenance Schedule List

This chapter will cover the following maintenance intervals:

 Weekly Maintenance
 Every Other Week Maintenance
 Monthly Maintenance

Flowcharts are provided for weekly, every other week, and monthly procedures in this
chapter. These flowcharts are available as a quick reference for the procedures.

Review the User’s Guide for Warnings, Cautions, Important, Note, and Tip alerts as well
as step-by-step instructions.

For information on maintenance intervals beyond Monthly (Every Other Month,

Quarterly, 6-Month, Yearly, As Needed), refer to the AU5800 Chemistry Analyzer User’s
Guide, Chapter 8: Maintenance.

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Maintenance Resources

Multiple resources are available to assist in performing maintenance on the analyzer:

 User’s Guide
 Includes step-by-step instructions with pictures and drawings for all maintenance
procedures that can be performed by the operator
 Includes Warnings, Cautions, Important, Note, and Tip alerts
 Includes a list of supplies required for each procedure as well as the part number
of analyzer components that need to be replaced

 Maintenance Videos
 Available in the Online Help Video Directory of the analyzer
 Includes videos for the following maintenance procedures:
 Clean the Wash Nozzle Unit and Check the Tube Mounting Joints
 Replace the Photometer Lamp
 Replace Syringes or Syringe Case Heads
 Clean or Replace Individual Cuvettes
 Enhanced Cleaning of the ISE Electrode Line
 Manually Clean the ISE Mix Bar, Liquid Level Sensors, Sample Pot, and
Sample Pot Tubing
 Manually Clean the Drain Well and if Needed, Replace the Drain Tube

 Maintenance Job Aids

 Available on the Beckman Coulter website (see Chapter 9: Resources in this
manual)
 Shortened versions of maintenance procedures found in the User’s Guide
 Available for the following maintenance intervals
 Weekly and Every Other Week
 Monthly
 Two versions of job aids, one for procedures performed on their own, one for
efficiency (combines procedures and presented in efficient order)

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Weekly and Every Other Week Maintenance

Weekly Maintenance includes the following procedures:

 Clean the Sample Probe and Mix Bars
 Perform a W2
 Perform a Photocal
 Clean the Pre-dilution Bottles
 (ISE Option only) Check the Selectivity of the Na/K Electrodes
 (ISE Option only) Enhanced Cleaning of the ISE Electrode Line

(ISE Option only) Every Other Week or Every 3,000 Samples Maintenance includes the
following procedure:
 Manually Clean the ISE Mix Bar, Liquid Level Sensors, Sample Pot, and Sample
Pot Tubing

For Weekly Maintenance, the W2, Photocal, and Enhanced Cleaning of the ISE
procedures can be combined for efficiency. The flowchart in this chapter combines the
three procedures. These procedures can also be performed separately when needed.

Practice

 Use the flowcharts on the following pages as a quick reference for performing
Weekly and Every Other Week maintenance procedures

 The W2, Photocal, and Enhanced ISE Cleaning procedures take approximately an
hour to complete when performed together. Based on your laboratory workflow,
when will these procedures be performed?

 Every Other Week maintenance requires sonication of the sample pot and tubing for
10 minutes. Will your lab order spare sample pot and tubing sets to minimize
analyzer downtime?

 Identify the solutions that you will need for maintenance. Some of these solutions
need to be made up prior to performing the maintenance procedures

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Monthly Maintenance

Monthly Maintenance includes the following procedures:

 Clean the Sample Probe and Reagent Probe Wash Wells
 Clean the Mix Bar Wash Wells
 Clean the Wash Nozzle Unit and Check the Tube Mounting Joints
 Clean the DI Water Tank, DI Filter, and Sample Probe Filter

Practice

 Use the flowcharts on the following pages as a quick reference for performing
Monthly maintenance procedures

 The AU5800 Online Help has a maintenance video for the “Clean the Wash Nozzle
Unit and Check the Tube Mounting Joints” procedure. Watch the video for a
demonstration on performing the procedure

 The “Clean the DI Water Tank, DI Filter, and Sample Probe Filter” procedure
requires the sonication of DI water filters for 10 minutes. Will your lab order spare DI
water filters to minimize analyzer downtime?

 Identify the solutions that you will need for maintenance. Some of these solutions
need to be made up prior to performing the maintenance procedure

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 Chapter 7
As-Needed Tasks

Introduction

This chapter provides information on tasks that are performed on an as-needed basis.
The procedures include:

 Fix (assign) a reagent position

 Set calibrator lot concentrations
 Configure a new QC lot
 Define a user menu
 Save or load system parameters
 Save data to external media
 Perform an end process
 Perform and recover from an emergency stop
 Disable a test
 Transfer data to the host

These tasks may not be required for all operators of the AU5800. Determine which
tasks apply to you based on your role and responsibilities in the lab.

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Fix (Assign) a Reagent Position

If your lab will be using non-bar coded reagents (user-defined or from a third party
vendor) on the AU5800, you will need to assign these reagents to fixed positions. The
analyzer does not read bar codes on reagents in fixed positions. Fixed positions in the
reagent refrigerators can be identified by an asterisk (*) to the left of the “Pos.” column
in the Reagent Management: Details screen.

When reagents in fixed positions need to be replaced or replenished you do not need to
fix them again unless you are placing them into new positions. You can edit the
information for a fixed reagent position as-needed.

The Initialize Onboard Stability button can be used to reset the “Onboard Remaining”
time when a fixed reagent is replaced or replenished. For this feature to work, the fixed
reagent needs to have:
 An Onboard Stability time set for the test in Specific Test Parameters
 A lot number and bottle number (SN) entered for the reagent bottle

You can also assign bar coded reagents to fixed positions if the bar code is damaged or
unreadable. When fixing bar coded reagents that have R1 and R2 bottles, both bottles
need to be assigned to fixed positions, even if only one of them have bar code read
issues. The Reagent Management: Details tab will display a Mismatch error if the
information entered for fixed R1 and R2 reagents do not match.

Note: ID Edit F3 in the Reagent Management: Details screen can also be used to
manually enter the bar code for reagent bottles with bar code read errors. When using
ID Edit the position is not fixed. The system will generate a “REAGENT ID READ
ERROR” alarm each time a reagent check is performed, but it will remember the
manually entered bar code number as long as the bottle remains in the same position.

Do not leave positions fixed if they are no longer needed. Be sure to switch the position
back to Reagent ID so the system will read bar codes for reagents placed in the position
on the reagent refrigerator.

Practice

 Use the flowchart on the following page to identify how to fix a position, assign a
reagent to the fixed position, and load the reagent

 Will you have fixed reagent positions on your system? Identify which tests will be
fixed on your analyzer

 Will your lab policy require the entering of lot and bottle number (SN) for fixed
reagents? This is required to update onboard stability for fixed reagents

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 Fix (Assign) a Reagent Position

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Set Calibrator Lot Concentrations

When you receive a new lot of a calibrator in the lab you will need to enter the new
information (lot number, expiration, concentrations) into the system.

The calibrator kit includes an insert with the updated concentrations for tests in the
calibrator. Confirm the concentrations are entered correctly before processing
calibrations.

Practice

 Use the flowchart on the following page to practice updating a calibrator with new lot
information

 Will all operators be required to know how to enter new calibrator lot information on
the analyzer?

 How will you confirm that the correct calibrator lots are in use?

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 Set Calibrator Lot Concentrations

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Configure New QC Lot

When you receive a new lot of a QC in the lab you will need to enter in the new
information (lot number, expiration, mean, SD, range) on the system.

If your lab policy requires parallel lot testing for new QC lots you will need to configure
the new QC separately from the current QC. This will allow both lots of QC to be
ordered as a part of the automatic QC requisition.

Practice

 Use the flowchart on the following page to practice defining a new lot of QC

 Will all operators be required to know how to enter new QC lots on the analyzer?

 Will you use the QC Parameters to define new lots of QC on the analyzer, or will this
be managed on a different QC program (on a data management system or LIS)?

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 Configure a New QC Lot

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Define a User Menu

The User Menu allows for the customization of up to 16 menus (with operator-defined
names) frequently used in your lab. Selecting a menu from the User Menu can save
time for the operator by decreasing the number of steps required to reach specific
software screens.

Practice

 Use the flowchart on the following page to practice creating a user menu entry

 Does your analyzer already have some User Menu entries defined? Your
Applications Specialist may have defined some during installation

 Will you define User Menu entries on your system to access screens for routine
operations? Or will you define entries for less frequently accessed screens?

 Think of how you can name a User Menu entry to make software navigation easier
for operators

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 Define a User Menu

Select Menu List

Select System menu

Select User Menu sub-menu

Select Edit F1

Select the empty light blue box

Select the menu to put in the User Menu list from the
“Select Screen” drop-down list

Enter the user-defined menu name in the

“Display Data” field

Note: Up to 28 characters can be entered

on each of the two lines.

Select Entry

Select Confirm F1

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Save or Load System Parameters

The system can save or load parameters to a backup folder on the hard drive or
external media. Beckman Coulter recommends saving parameters to external media
when programming changes are made to the Parameters or System menus, or
following laboratory policy. If your lab needs to add, remove, or edit any tests following
the initial install of your system, it is important to save the updated test parameters to
external media to ensure that a current backup copy is available.

Having a system parameters backup can be useful in the event that you have a system
issue that requires the replacement of the computer. The saved parameters can be
quickly loaded onto the new computer. This would save a significant amount of
analyzer downtime that would be required if a backup was not available and the
information needed to be reentered.

Practice

 Use the flowchart on the following page to practice saving system parameters

 Will your lab have a policy to perform a system parameter backup on a routine
schedule? Who will be required to know how to save system parameters?

 What external media will you use for backup (flash drive, CD, floppy disk)?

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 Save of Load System Parameters

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Save Data to External Media

Save data to external media for a backup of data, or to transfer the data to another
computer. Data is saved to an “AU Data” folder. The data files are identified by the
index.

Sample data, reagent blank, calibration, and QC data can be saved to external media.

Practice

 Use the flowchart on the following page to practice saving data to external media

 Does your lab require data to be saved electronically? If so, how frequently will the
data backup be performed at the analyzer?

 Do you have other options for backing up data in your lab?

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 Save Data to External Media

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Perform an End Process (System Shutdown)

An End Process turns off the analyzer lamp and the computer. The reagent
refrigerators and incubator temperatures are maintained. The ISE module performs an
automatic prime with ISE MID Standard Solution every hour to keep the electrodes
conditioned while the system is shutdown.

An End Process can be initiated after a W2 and/or Photocal is started. In this case, the
system shuts down after the W2 and/or Photocal is completed.

An End Process (system shutdown) is recommended for labs that do not operate 24
hours a day, 7 days a week, to save lamp life.

An automatic startup time can be set for the system to automatically turn on at a
specified time each day of the week. This feature allows the system to warm up before
you arrive in the lab in the morning.

Practice

 Use the flowchart on the following page to practice performing an End Process and
setting a daily automatic startup time

 Will you be performing an End Process daily in your lab? If so, you can set an
automatic startup time so that the analyzer will turn on at a specified time each day
of the week

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 Perform an End Process (System Shutdown)

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 Program Automatic Startup Function

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Perform and Recover from an Emergency Stop

Pressing the EM STOP button on the analyzer immediately turns off power to the
analyzer and ISE module. All analysis immediately stops and any data that is not
complete is lost and must be repeated.

Reagents remain in the cuvettes after performing an emergency stop of the analyzer.
A W1 should be performed following an emergency stop to clean the cuvettes before
performing an End Process or resuming analysis.

The same steps used to recover from an emergency stop also apply to recovering from
a loss of power to the analyzer.

When the analyzer turns on following an emergency stop the system mode remains in
Warm up for 1.5 hours. The system requires a 20 minute warm up time for the lamp.
After the 20 minute warm up time you can bypass warm up to standby.

Practice

 Use the flowchart on the following page to practice performing an emergency stop

 When an emergency stop is performed on the analyzer the refrigerator temperature

is no longer maintained. Consider how to manage reagents in the refrigerators if the
system is not immediately turned back on

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 Perform and Recover from an Emergency Stop

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Disable a Test

Specific tests can be selected to stop analysis (disable) even when the test has an
order. It may be useful to disable a test if the calibration failed for that test, or QC fails
and samples are in process.

 A test can be disabled or enabled during Measure mode. Analysis of the test(s) will
stop or re-start after selection in the Disable dialog
 Reagent blank, calibration, and QC samples can still be processed for disabled tests
 Tests that are disabled display and print with a / flag, indicating the test was ordered
but not performed
 Selections in the Disable dialog are in effect until a new index is created, an End
Process is performed, or the test is re-enabled

Practice

 Use the flowchart on the following page to practice disabling a test

 Navigate to the Home menu to identify how an operator can see that a test is
disabled

 The test is re-enabled when a new index is created or following an End Process.
You can also re-enable the test from the Start Condition screen. Re-enable the test
you disabled

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 Disable a Test

Note: A test can be disabled or enabled

during Measure mode.

Select Home

Select Start Condition jump button

Select Disable F7
A list of tests to disable is displayed

Note: For analyzers with multiple units, Unit 1 tests

are displayed as default. Select the correct Unit #
for the test(s) you need to disable.

Select the test(s) to be disabled.

Tests that are disabled are highlighted in orange

Select OK

Note: Tests remain disabled until:

They are re-enabled
A new index is made, or
An End Process is performed

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Transfer to Host

The system is generally set up to automatically transfer results to the host. If the
communication between the analyzer and host becomes unavailable, you may need to
resend the results to the host once the communication is reestablished.

Practice

 Use the flowchart on the following page to practice sending results to the host

 What scenarios might arise in your lab that will require you to resend results to the
host?

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 Transfer to Host

Verify the system is in Standby mode

Select Home

Note:
Tests results that have Select Sample Manager jump button
transferred to the host
(data management Note: Select OK if a “Data Not Found”
system or LIS) display message appears.
an r flag.

Select Main tab

Transfer
Previous data from current Current
or previous
index?

Select Search F3 Data from the current index displays with all
samples selected (highlighted in blue)

Note: Deselect any samples that do not

Select a start date and an end date to need to be transferred to the host.
search in the “Index” drop-down list

Note: Sample Kind selection defaults to all

available in the index range selected Select Transfer to Online F7

Select OK Select OK

Data displays with all samples selected

(highlighted in blue)

Note: Deselect any samples that do not

need to be transferred to the host.

Select Transfer to Online F7

Select OK

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 Chapter 8
Troubleshooting

Introduction

This chapter introduces you to the resources available for troubleshooting on the
AU5800. The following will be covered in this chapter:

 Alarm list
 Error flags
 Troubleshooting reagent blank, calibration, and QC failures
 Troubleshooting tips for common issues

Practice

 Use the information in the following pages to:

 Review information in the Alarm List and Alarm Help
 Identify flags
 Review tools available for troubleshooting reagent blank, calibration, and QC
failures

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Alarms

 Alarm messages display on the bottom of the software screen with an audible alert
 Select Alarm Clear once to silence the audible alert
 Select Alarm Clear a second time to clear the message from the bottom
of the software screen

 There are three alarm levels. A different sound can be set for each alarm level:

Level Description

1 Warning: A fatal system abnormality exists

2 Caution: An abnormality influencing the data exists

Informational: No system abnormality and the system displays the

3
operation log

 System can store and display a maximum of 4096 alarms on the Alarm List

 To access detailed information about the alarm and possible corrective actions:
 Select Alarm List . The system displays the Alarm List dialog with the
most recent alarm at the top
 Select the alarm you are investigating, and then select Help. The system
displays the Alarm Help dialog for the selected alarm
 Select Print Screen on the keyboard to print a copy of the alarm
 Review the information in the User section for possible corrective actions to take
 Contact Beckman Coulter Technical Support if the issue is not resolved using the
available resources

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 The Alarm Message and Alarm sections display the general and specific content in
the parentheses and brackets for a selected alarm. The following example is for a
“Sample Short” alarm:
Alarm
Sample Short (A, B, C, D, E, F, G, H, I, J K) [A]
Message

Alarm Sample Short (CRE, 3, 0013, 1, ACAL, , 1, 0, 0, 4 1) [Unit 2]

Displays more information about the alarm, including a key to the content in the
parentheses and brackets. The following is the key for the alarm message above:
A: Test name F: Sample ID
B: Rack type (1: Routine, G: Detailed Error information
Comment* 2: Emergency, 3: ACAL, 4: QC, H: Remaining pulses for sample probe
5: Reagent blank, 7: Repeat descending
C: Rack ID I: Pulses for sample probe submergence
D: Cup position J: Cup type
E: Sample No. K: Probe information
Corrective action to be taken by the operator. If there is no information in the User
User section, determine if Beckman Coulter Technical Support should be contacted to help
resolve the issue.

*Note: Select the Detailed button if the Comment section does not provide enough information to
interpret the contents of the alarm.
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Flags

 The system generates flags when the system encounters a condition that can affect
the result. This condition can range from minor warnings to severe errors that
require immediate attention

 Up to four flags may be attached to a result according to priority. The four flags with
the highest priorities are displayed in red when viewing results from the
Sample Status: Detail screen or on the printed reports

 Review each flag and identify the root cause, and perform the corrective action

 Do not report any result with an unresolved or unexpected flag

 For a list of flag definitions, possible causes, and corrective actions, refer to:

Reference: User’s Guide

 Chapter 9: Error Flags
 9.1 Summary or Error Flags (Alphabetical order)
 9.2 Summary of Error Flags (In order of priority)
 9.3 Error Flag Details

 Flag definitions are also displayed in the <Contents of Error> area when viewing
results from the Sample Status Detail screen. Select a result with flags to see the
definitions at the bottom of the screen

 Examples of how flags are displayed on different software screens and on a printed
report:

Sample Status:
Detail

Sample Status:
Realtime Display

Sample Manager:
Sample tab

Data Log Report

(Printed report)

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Troubleshooting Reagent Blank, Calibration, and QC Failures

Reagent  The system generates “RB DATA ERROR” alarms if the reagent
Blank (RB) blank fails for a test
Issues
 Review the printout for flags:
 Flags u or y display if the RB data of the first read point of the
test fails
 Flags U or Y display if the RB data of the second read point of the
test fails

 Review the RB history data graph in the Calibration Monitor:

RB History
 OD readings should be consistent with previous reagent blank
data

 If RB fails for only one test, inspect what is UNIQUE to the failed
test:
 Reagent
 Reagent expiration date
 Reagent on-board expiration date
 Confirm correct reagent preparation
 Confirm fixed reagents are in the correct position
 Confirm a bar code labeled reagent is not in a position fixed
for a different test

 If RB fails for multiple tests, inspect what is COMMON to the failed

tests:
 Reagent Probes
 Reagent Syringes
 Cuvette wheel (Inner or Outer)
 Analyzer Unit (for systems with multiple units)
 RB sample (DI water)

 Take action based on your investigation, and perform a reagent

blank and calibration

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Calibration  The system generates “CALIBRATION FACTOR RANGE
(CAL) OVER/UNDER” and “CALIBRATION ERROR” alarms if the
Issues calibration fails for a test

 Review the printout for flags

Note: A failed calibration does not always generate a flag for the test
on the calibration data report.

 Review the calibration history data graph in the Calibration Monitor:

Calibration History
 OD readings should be consistent with previous calibration data
 Look for separation between calibrator levels for a multi-point
calibration
 Look at the RB History of the test to determine if you see the
same trend (OD readings increase/decrease) as the failed
calibration. If so, use the RB Issues section to troubleshoot the
reagent before continuing with troubleshooting the calibrator or
sampling system

 If CAL fails for only one test, and the RB history OD readings for
the test are consistent, inspect what is UNIQUE to the failed test:
 Calibrator
 Confirm the correct calibrator was poured
 Confirm the integrity of the calibrator material (expiration date,
open bottle stability, time at room temperature, contamination)
 Confirm correct calibrator is placed in the correct position
 Confirm the correct lot number is in use and concentration set
points entered correctly

 If CAL fails for multiple tests, inspect what is COMMON to the

failed tests:
 Calibrator
 Sample Probe
 Sample Syringe
 Wash Syringe
 Cuvette wheel (Inner or Outer)
 Analyzer Unit (for systems with multiple units)

 Take action based on your investigation, and perform a reagent

blank and calibration

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QC Issues  Perform QC on the system to validate the calibration

 If the QC Mode on the system is set for Preset or Cumulative, the

system generates “QC DATA RANGE OVER/UNDER” alarms if the
results exceed the QC range

 Review the printout for flags

 QC data that exceeds Single Check Level generates1Q flag
 QC data that does not meet Multi Check Level selection
generates 1Q to 7Q flags

 If QC fails, review the following for the failed tests:

 Reagent blank issues
 Calibration issues

 If the RB History and Calibration History OD data are consistent for

the tests that failed QC, troubleshoot the QC material:
 QC Sample:
 Confirm the correct QC material was poured
 Confirm the integrity of the QC material (expiration date, open
bottle stability, time at room temperature, contamination)
 Confirm correct QC material is placed in the correct position
 Confirm the correct QC lot number is in use and range is
entered correctly

 Take action based on your investigation:

 Perform a reagent blank and calibration, if needed
 Process QC for the failed tests

Other  DI water
Consider-  Waste
ations  Power
 Temperature
 Humidity
 Mixing
 Cuvette overflow
 Incorrect wash dilution

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Troubleshooting Tips

The following pages include flowcharts for troubleshooting common issues that may
arise when operating the analyzer. The flowcharts included are:

 Reagent Liquid Level Detection Error Troubleshooting Tips

 Rack Jam Troubleshooting Tips
 Failed Reagent Blank (RB), Calibration (CAL), or Quality Control (QC)
Troubleshooting Tips
 ISE Calibration Failure Troubleshooting Tips

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 Chapter 9
Resources

Introduction

This chapter identifies and describes resources available to assist you with your
AU5800 chemistry analyzer.

Beckman Coulter Support Personnel

Technical Support (Hotline):

 Provides 24 hour phone support
 Contact at 1-800-854-3633
 Know your System ID _____________________________
 www.beckman.com/help  Support Request

Applications Specialist:
 Provides installation support
 Your applications specialist is ___________________________
(Name)

Field Service Engineer:

 Installs the system
 Repairs and assists in maintaining your system
 Your Field Service Engineer is __________________________
(Name)

PROService
 PROService is an optional feature that allows operators to manually transmit
parameter, data, log, and other system files to the Beckman Coulter service center
for remote diagnostics and troubleshooting. Contact your Beckman Coulter
representative for more information about getting PROService
 If you have PROService, refer to the following for instructions on how to transmit
files from the AU5800 to the Beckman Coulter service center:

Reference: AU5800 Chemistry Analyzer User’s Guide

 Chapter 7: System Monitoring and Results
 7.16 Using Beckman Coulter PROService (Option)

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References

Beckman Coulter website at www.beckmancoulter.com:

 Sign up for “My Technical Documents” to receive updates on documents pertaining

to your analyzer. Access the My Technical Documents User Guide link from the
website at SUPPORT  Technical Documents for instructions on how to sign up

 AU5800 User’s Guide and Quick Response Guide can be found using the
following pathway from the website:
 SUPPORT  Technical Documents  make the following selections from the
Search By Product drop-down lists:
 Market Segment: Diagnostics
 Product Line: Chemistry
 Product Series: AU Systems
 Product: AU5800
 Document Category: Instrument IFU/Manual

 AU5800 Reagent Instructions for Use and Chemistry Setting Sheets can be
found using the following pathway from the website:
 SUPPORT  Technical Documents  make the following selections from the
Search By Product drop-down lists:
 Market Segment: Diagnostics
 Product Line: Chemistry
 Product Series: AU Systems
 Product: AU Test Menu
 Document Category: Consumable IFU/CIS/Setting Sheet
 To search for documents for a specific test, use the Search by
Reagents/Calibrators/Controls or Search By Item/REF/Document Number
options

 AU5800 Safety Data Sheets (SDS/MSDS) can be found using the following
pathway from the website:
 SUPPORT  Technical Documents  Safety Data Sheets (SDS/MSDS) tab 
enter Search By and Search For criteria

 AU5800 Tools, Job Aids, and E-learning can be found using the following pathway
from the website:
 SUPPORT  Training & Education  Diagnostics  Chemistry  AU5800:
 Tools & Aids
 Job Aids (Daily Start Up, Maintenance, System Operations)
 Competency Checklist
 Troubleshooting Tips
 On Demand
 AU5800 E-learning module

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 Chapter 10
In-Lab Training Competency Exercise

The competency exercise should be completed by the end of the in-lab training session.
All materials supplied with the AU5800 or used during training may be used to complete
this exercise. The exercise is a combination of performing learned skills and knowledge
review.

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Analyzer Overview

1. Identify how many of each component listed below is on an ISE unit (for 1 flowcell)
and one analyzer unit:

Number on one Number on an ISE Unit

Components
Analyzer Unit (1 flowcell)
Sample Probes
Reagent Probes
ISE Mix Bar
R1/S Mix Bars
R2 Mix Bars
Syringes
Electrodes
Cuvettes

2. Place a number next to each step in the order they occur in the sample processing
of a photometric test on the analyzer (1 = first, 2 = second, etc.):

Order Step
The cuvette is washed, rinsed, and dried by the wash nozzle unit

The sample probe delivers sample into the cuvette

The rack is moved to the sample aspiration position

The R2 mix bar mixes the R2 reagent, sample, and R1 reagent.
The photometer continues to take readings
The reagent probe delivers R1 reagent (concentrated) and diluent
into the cuvette
The sample mix bar mixes the sample and R1 reagent. The
photometer continues to take readings
The rack is moved to the bar code reader where sample
programming is identified
If necessary, the reagent probe delivers R2 reagent (concentrated)
and diluent into the cuvette
The R1 mix bar mixes the diluted R1 reagent. The photometer
starts taking readings

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Software Overview

3. Identify the name of each Main Button Area button.

Hint: When you hold the mouse pointer over the button on the software screen, a
pop-up message will indicate the name of the button.

Button Name Button Name

4. Which button can you select to access the User’s Guide to look up how to perform
a maintenance procedure? _____________

5. Your analyzer is in a Stop mode. Which button do you select to bring the analyzer

back to Standby? ____________________

6. Which menu should you be in to access the Sample Status button to view the
status of samples processing? ____________________

7. The lamp needs to be replaced on the analyzer. Which button should you select to
shut down the analyzer? _______________

8. Racks have been placed on the rack set unit while the analyzer is in Standby.
Which button should you select to place the system into Measure? _____________

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Daily Start Up

9. Identify the solution used in the bottles listed below and the probe that utilizes that
solution. The bottles are located near the sample and reagent probes on the
analyzer.

Hint: You may need to look on the analyzer to identify which probe is used for each
solution.

Sample Probes or
Bottle Solution
Reagent Probes?
Diluent/W2
CLN-1
CLN-2
DET-1/W2
DET-2

10. Where should you go to view the system temperatures? Circle the correct answer.

a. Start Condition

b. Analyzer Status

c. Analyzer Maintenance

d. Sample Status

11. (ISE Labs only) Identify the reagents used by the ISE module:
 _______________________
 _______________________
 _______________________

12. (ISE Labs only) TRUE or FALSE?

You need to press the green DIAG button on the analyzer to start the ISE Cleaning
procedure.

13. (ISE Labs only) Where is ISE calibration processed?

__________________________________________________________________

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Daily Start Up, continued

Refer to the Reagent Management screen above to answer the following questions:

14. TRUE or FALSE? A reagent check is required on the analyzer.

15. How many shots are available for Total Protein (TP)? _______________________

16. Which reagent(s) does not meet Reagent Inventory requirements? _____________

17. Which reagent(s) has a reagent short status? _____________________________

18. Which reagent(s) is missing, expired, or empty? ___________________________

19. What should you select to identify the Onboard Stability of reagents?
________________________

20. Why is COCA+ highlighted in gray?

__________________________________________________________________

What should you select to view the status of COCA+? _____________

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Daily Start Up, continued

21. Identify the four options available when performing a reagent check:
 __________________________
 __________________________
 __________________________
 __________________________

Circle the option(s) that can be used to check the volume of the solutions in the
bottles by the sample and reagent probes (Diluent, DET-1, DET-2, CLN-1, CLN-2)

22. During a reagent check, the system automatically orders reagent blank (RB) and
calibration (CAL) for all tests with: Check all that apply

Reagent blank or calibration expired

Reagent blank or calibration expires soon (time determined by lab)
New bottle or lot number for reagent
Reagent blank or calibration failed
QC failed

23. You can load multiple bottles of the same reagent on an analyzer unit. The AU
identifies each bottle as a sequence. Up to how many bottle sequences of the same
reagent can be on an analyzer unit at one time? Circle the correct answer.
a. 4
b. 5
c. 6
d. There is no limit

24. The system does not automatically process QC on multiple bottles of a reagent.
Which software pathway should you take when you need to order QC on multiple
bottles of the same reagent? Circle the correct answer.

a. QC > Rack Requisition > Start Entry > Individual Requisition

b. Rack Requisition > QC > Start Entry > Individual Requisition

c. Rack Requisition > QC > Individual Requisition > Start Entry

d. Rack Requisition > Individual Requisition > QC > Start Entry

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25. Perform a Daily Start Up on your AU5800 analyzer.

26. Review the items that you have covered in Chapter 3: Daily Start Up. Place a check
next to items you feel comfortable performing. Meet with your trainer to review or
answer any questions you may still have on unchecked items.

Daily Start Up Review Checklist

Set a new index
Perform daily analyzer maintenance
Inspect syringes (ISE and analyzer units) for leaks
Inspect the wash solution and replenish, as needed
Inspect the printer and paper
Inspect the stability of the upper cover
Inspect, clean, and prime the sample probes (ISE and analyzer units),
reagent probes, and mix bars
Replace the DI water in the pre-dilution bottles
Prepare the sample probe wash solutions (2% wash)
Replenish contamination parameter solutions, as needed
Verify the handle on the diluted wash solution tank is in the open position
Check the analyzer status
Check and replenish reagents
Perform an ISE start up
Check and load ISE reagents, as needed
Perform ISE cleaning
Perform ISE calibration
Perform analyzer calibration
Process quality control (QC)

27. Use the Calibration Monitor and QC Monitor to review the status of calibration and
QC from the daily start up.

28. Review the items for monitoring calibration and QC status and results in
Chapter 5: Monitor and Review Results. Place a check next to items you feel
comfortable performing. Meet with your trainer to review or answer any questions
you may still have on unchecked items.

Monitor and Review Results Checklist – RB/CAL/QC

Print RB/CAL/QC results
Review calibration using Calibration Monitor
Review QC using QC Monitor and QC Data Review

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Sample Processing and Programming

29. Place the letter representing the rack color next to the sample kind the rack is used
for:

Letter /
Letter Sample Kind
Rack Color
A: White Reagent Blanks

B: Yellow QC Samples

C: Green Emergency Samples

D: Orange Calibrator Samples

E: Red Manual Repeats

Routine Patient and
F: Blue
Automatic repeats

30. Circle the statement that is false when loading racks on the rack set unit:

a. Caps should be removed from all sample containers

b. The racks should be placed in the rack set tray with the rack bar code facing the
right of the analyzer

c. 13x75, 13x100, and 16x100 tubes can be placed in the same rack if they are for
the same sample type

d. When calibrating, always place the yellow racks in front of the blue rack

e. Manually programmed samples require a bar code if the analyzer is set for
Barcode analysis

31. TRUE or FALSE?

Samples placed in an Emergency (red) rack will have higher processing priority than
samples placed in a Routine (white) rack.

32. Which button do you select to start sample processing of racks loaded in the priority
rack set unit? ____________________

Can this button be selected while the system is in Measure 1 mode? ___________

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33. Which software screens can you use to view patient results? Check all that apply.

Sample Status > Status

Sample Status > Detail
Sample Status > Realtime Display
Sample Manager > Main tab
Sample Manager > Sample tab

34. Practice the following sample-related tasks, as appropriate, based on your analyzer
setup and lab processes:
 Manually program and process samples using the rack set unit for the sample
types used in your lab
 Manually program and process samples using the priority rack set unit for the
sample types used in your lab
 Recall and reprint a patient report

35. Review the items that you have covered in the Chapter 4: Sample Processing and
Programming and Chapter 5: Monitor and Review Results. Place a check next to
items you feel comfortable performing. Meet with your trainer to review or answer
any questions you may still have on unchecked items.

Sample Processing and Programming Checklist

Identify sample rack usage
Identify validated sample containers
Process bar coded samples with LIS-programming using the rack set unit
Process bar coded samples with LIS-programming using the priority rack set unit
Manually program and process samples in routine (white) racks
Manually program and process samples in emergency (red) racks

Monitor and Review Results Checklist – Patient Samples

Monitor patient results from Sample Status
Review and reprint patient results from Sample Manager

36. Practice and review any additional tasks from the In-Lab Training Manual required of
your role and responsibilities in your lab.

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37. You have completed the competency check. Have your trainer compare the
answers of your completed competency check with the Reference Key and review
any questions you might have.

CONGRATULATIONS!

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 Congratulations
_________________________________________________

has successfully completed the

In-Lab Training Course on the operation of the
AU5800 Chemistry Analyzer

________________________________________ ____________________
Beckman Coulter Representative Date

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In-Lab Training Competency Exercise Reference Key

Analyzer Overview

1. Identify how many of each component listed below is on the analyzer:

Number on one Number on an ISE Unit

Components
Analyzer Unit (1 flowcell)
Sample Probes 2 1
Reagent Probes 4 N/A
ISE Mix Bar N/A 1
R1/S Mix Bars 12 (6 of each) N/A
R2 Mix Bars 6 N/A
Syringes 8 3
Electrodes N/A 3
Cuvettes 408 N/A

2. Place a number next to each step in the order they occur in the sample processing
of a photometric test on the analyzer (1 = first, 2 = second, etc.):

Order Step
9 The cuvette is washed, rinsed, and dried by the wash nozzle unit

5 The sample probe delivers sample into the cuvette

2 The rack is moved to the sample aspiration position

The R2 mix bar mixes the R2 reagent, sample, and R1 reagent.
8
The photometer continues to take readings
The reagent probe delivers R1 reagent (concentrated) and diluent
3
into the cuvette
The sample mix bar mixes the sample and R1 reagent. The
6
photometer continues to take readings
The rack is moved to the bar code reader where sample
1
programming is identified
If necessary, the reagent probe delivers R2 reagent (concentrated)
7
and diluent into the cuvette
The R1 mix bar mixes the diluted R1 reagent. The photometer
4
starts taking readings

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Software Overview

3. Identify the name of each Main Button Area button.

Hint: When you hold the mouse pointer over the button on the software screen, a
pop-up message will indicate the name of the button.

Button Name Button Name

Home Menu List

User Menu Start

Pause Feeder Stop

Stop/Standby Help

Logout End

4. Which button can you select to access the User’s Guide to look up how to perform
a maintenance procedure? Help button

5. Your analyzer is in a Stop mode. Which button do you select to bring the analyzer

back to Standby? Stop/Standby button

6. Which menu should you be in to access the Sample Status button to view the
status of samples processing? Home

7. The lamp needs to be replaced on the analyzer. Which button should you select to
shut down the analyzer? End button

8. Racks have been placed on the rack set unit while the analyzer is in Standby.
Which button should you select to place the system into Measure? Start button

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Daily Start Up

9. Identify the solution used in the bottles listed below and the probe that utilizes that
solution. The bottles are located near the sample and reagent probes on the
analyzer.

Hint: You may need to look on the analyzer to identify which probe is used for each
solution.

Sample Probes or
Bottle Solution
Reagent Probes?
Diluent/W2 DI water Reagent
CLN-1 Lab dependent Reagent
CLN-2 Lab dependent Reagent
DET-1/W2 2% wash solution Sample
DET-2 2% wash solution* Sample

*Labs processing a high volume of samples or dialysis patient samples may

use Beckman Sodium Hypochlorite (5%) diluted 1:5 in the DET-2 bottles.

10. Where should you go to view the system temperatures? Circle the correct answer.

a. Start Condition

b. Analyzer Status

c. Analyzer Maintenance

d. Sample Status

11. (ISE Labs only) Identify the reagents used by the ISE module:
 ISE Buffer Solution
 ISE MID Standard Solution
 ISE Reference Solution

12. (ISE Labs only) TRUE or FALSE?

You need to press the green DIAG button on the analyzer to start the ISE Cleaning
procedure.

13. (ISE Labs only) Where is ISE calibration processed?

Positions labeled S-L, S-H, U-L, and U-H on the ISE unit

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Daily Start Up, continued

Refer to the Reagent Management screen above to answer the following questions:

14. TRUE or FALSE? A reagent check is required on the analyzer.

15. How many shots are available for Total Protein (TP)? 870 shots

16. Which reagent(s) does not meet Reagent Inventory requirements? ALB

17. Which reagent(s) has a reagent short status? PHOS

18. Which reagent(s) is missing, expired, or empty? MG, AST, TBILC

19. What should you select to identify the Onboard Stability of reagents?
Details tab

20. Why is COCA+ highlighted in gray?

It is not used for the displayed sample type (Serum) on the Main tab

What should you select to view the status of COCA+? Type drop-down

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Daily Start Up, continued

21. Identify the four options available when performing a reagent check:
 Check all positions
 Check specified positions
 Check changed positions
 Reset Only

Circle the option(s) that can be used to check the volume of the solutions in the
bottles by the sample and reagent probes (Diluent, DET-1, DET-2, CLN-1, CLN-2)

22. During a reagent check, the system automatically orders reagent blank (RB) and
calibration (CAL) for all tests with: Check all that apply

 Reagent blank or calibration expired

 Reagent blank or calibration expires soon (time determined by lab)
 New bottle or lot number for reagent
 Reagent blank or calibration failed
QC failed

23. You can load multiple bottles of the same reagent on an analyzer unit. The AU
identifies each bottle as a sequence. Up to how many bottle sequences of the same
reagent can be on an analyzer unit at one time? Circle the correct answer.
a. 4
b. 5
c. 6
d. There is no limit

24. The system does not automatically process QC on multiple bottles of a reagent.
Which software pathway should you take when you need to order QC on multiple
bottles of the same reagent? Circle the correct answer.

a. QC > Rack Requisition > Start Entry > Individual Requisition

b. Rack Requisition > QC > Start Entry > Individual Requisition

c. Rack Requisition > QC > Individual Requisition > Start Entry

d. Rack Requisition > Individual Requisition > QC > Start Entry

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25. Perform a Daily Start Up on your AU5800 analyzer.

26. Review the items that you have covered in Chapter 3: Daily Start Up. Place a check
next to items you feel comfortable performing. Meet with your trainer to review or
answer any questions you may still have on unchecked items.

Daily Start Up Review Checklist

Set a new index
Perform daily analyzer maintenance
Inspect syringes (ISE and analyzer units) for leaks
Inspect the wash solution and replenish, as needed
Inspect the printer and paper
Inspect the stability of the upper cover
Inspect, clean, and prime the sample probes (ISE and analyzer units),
reagent probes, and mix bars
Replace the DI water in the pre-dilution bottles
Prepare the sample probe wash solutions (2% wash)
Replenish contamination parameter solutions, as needed
Verify the handle on the diluted wash solution tank is in the open position
Check the analyzer status
Check and replenish reagents
Perform an ISE start up
Check and load ISE reagents, as needed
Perform ISE cleaning
Perform ISE calibration
Perform analyzer calibration
Process quality control (QC)

27. Use the Calibration Monitor and QC Monitor to review the status of calibration and
QC from the daily start up.

28. Review the items for monitoring calibration and QC status and results in
Chapter 5: Monitor and Review Results. Place a check next to items you feel
comfortable performing. Meet with your trainer to review or answer any questions
you may still have on unchecked items.

Monitor and Review Results Checklist – RB/CAL/QC

Print RB/CAL/QC results
Review calibration using Calibration Monitor
Review QC using QC Monitor and QC Data Review

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Sample Processing and Programming

29. Place the letter representing the rack color next to the sample kind the rack is used
for:

Letter /
Letter Sample Kind
Rack Color
A: White F Reagent Blanks

B: Yellow C QC Samples

C: Green E Emergency Samples

D: Orange B Calibrator Samples

E: Red D Manual Repeats

Routine Patient and
F: Blue A
Automatic repeats

38. Circle the statement that is false when loading racks on the rack set unit:

a. Caps should be removed from all sample containers

b. The racks should be placed in the rack set tray with the rack bar code facing the
right of the analyzer

c. 13x75, 13x100, and 16x100 tubes can be placed in the same rack if they are for
the same sample type

d. When calibrating, always place the yellow racks in front of the blue rack

e. Manually programmed samples require a bar code if the analyzer is set for
Barcode analysis

30. TRUE or FALSE?

Samples placed in an Emergency (red) rack will have higher processing priority than
samples placed in a Routine (white) rack.

31. Which button do you select to start sample processing of racks loaded in the priority
rack set unit? Start button

Can this button be selected while the system is in Measure 1 mode? Yes (as long

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32. Which software screens can you use to view patient results? Check all that apply.

Sample Status > Status

 Sample Status > Detail
 Sample Status > Realtime Display
Sample Manager > Main tab
 Sample Manager > Sample tab
33. Practice the following sample-related tasks, as appropriate, based on your analyzer
setup and lab processes:
 Manually program and process samples using the rack set unit for the sample
types used in your lab
 Manually program and process samples using the priority rack set unit for the
sample types used in your lab
 Recall and reprint a patient report

34. Review the items that you have covered in the Chapter 4: Sample Processing and
Programming and Chapter 5: Monitor and Review Results. Place a check next to
items you feel comfortable performing. Meet with your trainer to review or answer
any questions you may still have on unchecked items.

Sample Processing and Programming Checklist

Identify sample rack usage
Identify validated sample containers
Process bar coded samples with LIS-programming using the rack set unit
Process bar coded samples with LIS-programming using the priority rack set unit
Manually program and process samples in routine (white) racks
Manually program and process samples in emergency (red) racks

Monitor and Review Results Checklist – Patient Samples

Monitor patient results from Sample Status
Review and reprint patient results from Sample Manager

35. Practice and review any additional tasks from the In-Lab Training Manual required of
your role and responsibilities in your lab.

AU5800 In-Lab Training Manual Page 158 of 158

Version 1.0 (May 2016) Beckman Coulter Professional Development