CHEMICAL ANALYSIS OF SOFT WATER

1.0 Description: _______________________________________________________________

_______________________________________________________________

Result: Sign. /Date Complies / Does not Comply

______________________________________________________________________________________

2.0 pH: (Limit: 6.5 to 8.5)

Report Attached Inst. ID.________

To ______ ml sample added _______ ml of saturated solution of KCl and determined pH with
calibrated pH meter
Observation:

Result: Sign. /Date Complies / Does not Comply

_______________________________________________________________________________________

3.0 Hardness: (Limit : 10 ppm )

Take ____ ml sample in a conical flask added ______ml purified water, ____ ml of ammonia buffer
solution and pinch of mordant black II mixture and shake it. Titrate with 0.01 M EDTA solution.
Consumption of 0.01 M EDTA: ml
Calculation= TV x N x 0.0010008 x 106
V x 0.01
Observation:
Result: Complies / Does not Comply Sign/Date
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 REPORT OF RAW WATER / SOFT WATER

Sample Name : A.R. No. :

Location of Point : Sampled by
Date of testing: Sampled quantity
Date of Analysis : Date of Completion :
Test Observation
Total Aerobic Viable Count
E. coli

Salmonella

P. aeruginosa
Pathogens
S. aureus

Enterobacteria

1. 0 Total Viable Count:

Media Used:

Sr.No. Media Name Sterilization Lot No.

1. R2A / Plate Count Agar

Sample preparation: 1 ml of water sample pippeted in petri dish, and poured sterile R2A / PCA (40-
45ºC).
Medium : 20-30ml R2A / plate count agar.

Incubation : 30-35C for 120 hrs.

Observation : a. ________________b. _______________mean __________ CFU/ml

Complies / does not comply Analyzed by date

2. 0 Test for Pathogens

Media Used:

Sr.No. Media Name Sterilization Lot No.

1. Soyabean Casein digest medium
2. MacConkey broth
3. MacConkey Agar
4. EMB Agar
5. Rappaport Vassiliadis Salmonella Enrichment Broth
6. Enterobacteria Enrichment Broth
7. VRBGA
8. Triple Sugar Iron Agar
9. Xylose-lysine Deoxycholate Agar
11. Cetrimide Agar
14 Mannitol Salt Agar
 Pathogens Testing:

Preparation of sample : Filter of water sample through 0.45 µ sterile filter paper , transfer the filter
paper in 100ml sterile Soyabean casein digest medium and incubate at 30-35°C for 18 to 24 hrs
(Solution-A).

1. E-coli :

Incubation
Observation
Sr.No. Test Temp Duration Test Negative
(hrs.) Positive Done by
(C) control
control Sign. / date
1ml of solution
A in 100ml
1. 30 -35 24- 48
MacConkey
broth
Streak on
2. MacConkey 30 -35 18- 72
agar plate
Streak on Eosin
3. Methylene Blue 30-35 18-24
agar plate

Observation: Complies / does not comply

2. Salmonella:

Incubation
Observation
S.
Test Temp Duration Test Negative Done by
No. Positive
(C) (hrs.) control Sign. /
control
date
0.1ml of solution A in
10ml Rappaport
1. Vassiliadis Salmonella 30 -35 24- 48
Enrichement Broth
tube.

Streak on 30 -35 24- 48

Xylose Lysine
Deoxycholate Agar
2.
plate

30 -35 24- 48
Streaking and stab
culture on
3. Triple sugar Iron Agar
Slant tube

Observation: Complies / does not comply

3. Pseudomonas aeruginosa:

Incubation
Observation
S.
Test Temp Duration
No. Test Negative
(C) (hrs.) Positive Done by
control
control Sign. / date

Streak loop full of

1. solution A on Cetrimide
30 -35 18- 72
agar
2. Oxidase Disk NA NA

Observation: Complies / does not comply

4. Staphylococcus aureus:

Incubation Observation
S.
Test Negative
No. Temp Duration Test Positive Done by
control
(C) (hrs.) control Sign. / date
Streak loop full of
1. solution A on Mannitol
30 -35 18-72
salt agar

Loop full culture in 0.5

2.
ml Mamalian Plasma 30 -35 24

Observation: Complies / does not comply

1.0 Test for Bile Tolerant gram negative bacteria:

S.
Test Incubation
No
Done by Observed by
(Date / Sign.) (Date / Sign.)
Temp. (0c) Duration (hrs)
Dissolved…… gm/ml of
1. sample in…… ml Soyabean
Casein digest broth.
20 - 25 2-5

5.1 Qualitative evaluation:

Incubation
Observation

S. No Test Test Done by

Duration Positive Negative
Temp. (0c) Sign. /
(hrs) control control
date
Transfer ……. ml in
…….. Enterobacteria 30-35
1. 24-48
enrichment broth -
Mossel.
Streak on Violet red 30-35
2. 18-24
bile glucose agar

Analyzed by / Date: Approved by / Date: