BRAND Catalog 2018
BRAND Catalog 2018
Worldwide.
General Catalog 900
®
Trademark Index
accu-jet®, BIO-CERT®, BLAUBRAND®, BRAND®, BRANDplates ®,
cell-culture™, cellGrade™, Dispensette®, EASYCAL™, HandyStep®,
hydroGrade™, immunoGrade™, inertGrade™, lipoGrade™, nano-cap™,
PLASTIBRAND®, pureGrade™, QuikSip™, SafetyPrime™, seripettor®,
Titrette®, Transferpette®, as well as the logo design marks depicted here,
are trademarks of BRAND GMBH + CO KG, Germany.
The following brands and trademarks as referenced in the current catalog are
property of their respective third-party owners as listed below:
AMERSHAM ®
GE HEALTHCARE LIMITED, GB
APPLIED BIOSYSTEMS® Applied Biosystems LLC, USA
AR-GLAS® SCHOTT AKTIENGESELLSCHAFT, Germany
AutoRep™ Rainin Instrument, LLC, USA
BECKMAN® Beckman Coulter, Inc., USA
BIOHIT® Sartorius Biohit Liquid Handling Oy, Finland
BIOMETRA® Biometra Biomedizinische Analytik GmbH, Germany
BIORAD® BIO-RAD LABORATORIES GMBH, Germany
Combitips® Eppendorf AG, Germany
COULTER COUNTER® BECKMAN COULTER INC., USA
EDISONITE® Schülke & Mayr GmbH, Germany
Encode™ Rainin Instrument, LLC, USA
Eppendorf® Eppendorf AG, Germany
FINNPIPETTE® Thermo Fisher Scientific Oy, Finland
GILSON® Gilson, Inc., USA
LightCycler® Roche Diagnostics GmbH, Germany
MegaBace® GE Healthcare Bio-Sciences AB, GB
METTLER TOLEDO® Mettler-Toledo AG, Switzerland
MICROSOFT® MICROSOFT CORPORATION, USA
MJ RESEARCH® BIO-RAD LABORATORIES, INC., USA
Mucasol® Schülke & Mayr GmbH, Germany
Mucocit® Schülke & Mayr GmbH, Germany
Multipette® Eppendorf AG, Germany
MWG® MWG BIOTECH AG, Germany
OHAUS® OHAUS CORPORATION, USA
PARAFILM® Bemis Company, Inc., USA
PEQLAB® PEQLAB Biotechnologie GmbH, Germany
Pursept® Schülke & Mayr GmbH, Germany
REPETMAN™ Gilson S.A.S., France
Roche® F. Hoffmann-La Roche AG, Switzerland
sartorius® SARTORIUS AG, Germany
STRATAGENE® Agilent Technologies, Inc., USA
TaKaRa® TAKARA SHUZO CO., LTD., Japan
TECHNE® TECHNE INCORPORATED, USA
TRANSGENOMIC® Transgenomic, Inc., USA
TRITON™ Dow Chemical Company, USA
WINDOWS® MICROSOFT CORPORATION, USA
About the Product
Our products are available through The catalog is intended for informational The DAkkS-accredited calibration laboratory
major laboratory supply dealers purposes only. Actual results may vary based at BRAND is authorized to issue DAkkS cali-
throughout the world. If you have upon a number of factors beyond our control, bration certificates for the following products:
trouble locating a specialized supplier including user technique or lab environment. Liquid Handling instruments, such as piston
in your area, just call – or visit us at Suitability for a specific application is the operated pipettes and burettes, dispensers
www.brand.de. responsibility of the end-user. and dilutors, glass and plastic volumetric
Specifications, catalog numbers and appear- instruments and glass density bottles.
ance are subject to change without notice.
Pack quantities are according to the recom- You can obtain an order form for repair and
mended order quantity. The minimum order calibration service online at www.brand.de.
quantities are indicated in the current pricelist.
Products certified as "suitable for cer- We will not be held responsible for print-
tification" satisfy the requirements of the ing or typographical errors.
German State Office of Weights and Mea-
sures ("Eichamt").
Contents
About us ............................................................................................................................ 4
Ideas
Partnering for success.
4 info@brand.de
About us
BRAND in Wertheim
6 info@brand.de
About us
www.brand.de 7
About us
8 info@brand.de
1949 1950
1957 1961
1967 1973
1963 1964
1979 1984
1986
1985
2011
1994
2005
1995 2008
2013
2012 2013
About us
Goals
With you in mind, we look to the future.
10 info@brand.de
© Mark Weathers Images/Flicks/Getty Images
About us
Advances
Vision, with implementation.
12 info@brand.de
© Peter de Rooij/Flickr/Getty Images
Product News
BRANDplates®
Insert System
Innovative cell culture
plates and inserts for cell
and tissue culture work.
• starting on page 159
D i s p e n s e t t e ® TA
The new bottle-top dis-
penser for trace analysis.
• pages 29-32 P LT U n i t
The pipette leak testing
unit for single and multi-
channel air-displacement
pipettes.
• pages 105-108
14 info@brand.de
Product News
Ti p R a c k –
Ti p B o x –
Ti p S t a c k ™
More News
macro pipette controller in new design, page 100
1.5 ml microcentrifuge tubes with lid closure, page 121
Strips of 8 PCR tubes with individual transparent flat caps, page 128
PCR plates in various formats, page 130
96-well deep-well plates 1.2 ml with elevated rim, page 137
and much more.
www.brand.de 15
© Tadao Yamamoto/amana images/Getty Images
Liquid Handling
One of the most important, fundamental activities
in modern laboratories is the careful handling of the
ever smaller quantities of liquids used. The precision
transfer of liquids in quantities from milliliters down to
nanoliters is still a challenge today.
Their impressive ergonomic design, high preci-
sion, and superb quality have contributed to making
BRAND liquid handling instruments known around the
world for excellence.
Overview
Bottle-top dispensers
Dispensette ®
Liquid Handling
from page 19
seripettor ®
Bottle-top burette
Titrette ®
from page 39
Repetitive pipettes
and PD-Tips
HandyStep ®
from page 87
Pipetting aids
accu-jet ®
from page 97
macro, micro
Bottle-top aspirator
QuikSip ™
from page 103
Calibration software
EASYCAL ™
from page 109
18 info@brand.de
Dispensette ® III
Dispensette ® Organic
Dispensette ® TA NEW!
Dispensette ®
proven itself the world over with its wide
range of practical applications.
It has been continuously improved over de-
cades to meet the increasing demands of Bottle-top Dispenser
the laboratory.
Dispensette® III
Dispensette® Organic
Areas of application
(For assistance in selecting a system, please see the guide on page 23)
Dispensette® III
Dispensette® Organic
20 info@brand.de
Dispensette® III
Dispensette® Organic
A Closer Look...
Liquid Handling
The Digital · Easy Calibration type has a digital
display and all the features that make dispensing
safer and convenient.
Mechanical/digital display
The mechanical volume setting is Easy Calibration Technique
easy to read and ensures accurate Calibration and adjustments accord-
and reproducible volume control. ing to ISO 9001 and GLP are done
within seconds. Alteration of factory
setting is automatically indicated by
a red recalibration flag. For more
information please see page 324.
www.brand.de 21
Dispensette® III
Dispensette® Organic
22 info@brand.de
Dispensette® III / Organic
Dispenser Selection Chart
Liquid Handling
Reagent Disp. III Disp. Reagent Disp. III Disp. Reagent Disp. III Disp.
Organic Organic Organic
Acetaldehyde + + Cyclohexane + Methylene chloride +
Acetic acid (glacial), 100% + + Cyclohexanone + + Mineral oil (Engine oil) + +
Acetic acid, ≤ 96% + + Cyclopentane + Monochloroacetic acid + +
Acetic anhydride + Decane + + Nitric acid, ≤ 30% + +
Acetone + + 1-Decanol + + Nitric acid, 30-70% */ ** +
Acetonitrile + + Dibenzyl ether + + Nitrobenzene + +
Acetophenone + Dichloroacetic acid + Oleic acid + +
Acetyl chloride + Dichlorobenzene + + Oxalic acid +
Acetylacetone + + Dichloroethane + n-Pentane +
Acrylic acid + + Dichloroethylene + Peracetic acid +
Acrylonitrile + + Dichloromethane + Perchloric acid + +
Adipic acid + Diesel oil (Heating oil), Perchloroethylene +
+
Allyl alcohol + + bp 250-350 °C Petroleum, bp 180-220 °C +
Aluminium chloride + Diethanolamine + + Petroleum ether, bp 40-70 °C +
Amino acids + Diethyl ether + Phenol + +
Ammonia, ≤ 20% + + Diethylamine + + Phenylethanol + +
Ammonia, 20-30% + 1.2 Diethylbenzene + + Phenylhydrazine + +
Ammonium chloride + Diethylene glycol + + Phosphoric acid, ≤ 85% + +
Ammonium fluoride + Dimethyl sulfoxide (DMSO) + + Phosphoric acid, 85% +
+ +
Ammonium sulfate + Dimethylaniline + Sulfuric acid, 98%, 1:1
n-Amyl acetate + + Dimethylformamide (DMF) + + Piperidine + +
Amyl alcohol (Pentanol) + + 1.4 Dioxane + Potassium chloride +
Amyl chloride (Chloropentane) + Diphenyl ether + + Potassium dichromate +
Aniline + + Essential oil + Potassium hydroxide +
Barium chloride + Ethanol + + Potassium permanganate +
Benzaldehyde + + Ethanolamine + + Propionic acid + +
Benzene (Benzol) + + Ethyl acetate + + Propylene glycol (Propanediol) + +
Benzine (Petroleum benzin), Ethylbenzene + Pyridine + +
+
bp 70-180 °C Ethylene chloride + Pyruvic acid + +
Benzoyl chloride + + Fluoroacetic acid + Salicylaldehyde + +
Benzyl alcohol + + Formaldehyde, ≤ 40% + Scintilation fluid + +
Benzylamine + + Formamide + + Silver acetate +
Benzylchloride + + Formic acid, ≤ 100% + Silver nitrate +
Boric acid, ≤ 10% + + Glycerol + + Sodium acetate +
Bromobenzene + + Glycol (Ethylene glycol) + + Sodium chloride +
Bromonaphthalene + + Glycolic acid, ≤ 50% + Sodium dichromate +
Butanediol + + Heating oil (Diesel oil), Sodium fluoride +
+
1-Butanol + + bp 250-350 °C Sodium hydroxide, ≤ 30% +
n-Butyl acetate + + Heptane + Sodium hypochlorite +
Butyl methyl ether + + Hexane + Sulfuric acid, ≤ 98% + +
Butylamine + + Hexanoic acid + + Tartaric acid +
Butyric acid + + Hexanol + + Tetrachloroethylene +
Calcium carbonate + Hydriodic acid, ≤ 57% ** + + Tetrahydrofuran (THF) * / ** +
Calcium chloride + Hydrobromic acid + Tetramethylammonium hydroxide +
Calcium hydroxide + Hydrochloric acid, ≤ 20% + + Toluene +
Calcium hypochlorite + Hydrochloric acid, 20-37% ** + Trichloroacetic acid +
Carbon tetrachloride + Hydrogen peroxide, ≤ 35% + Trichlorobenzene +
Chloro naphthalene + + Isoamyl alcohol + + Trichloroethane +
Chloroacetaldehyde, ≤ 45% + + Isobutanol + + Trichloroethylene +
Chloroacetic acid + + Isooctane + Trichlorotrifluoro ethane +
Chloroacetone + + Isopropanol (2-Propanol) + + Triethanolamine + +
Chlorobenzene + + Isopropyl ether + + Triethylene glycol + +
Chlorobutane + + Lactic acid + Trifluoro ethane +
Chloroform + Methanol + + Trifluoroacetic acid (TFA) +
Chlorosulfonic acid + Methoxybenzene + + Turpentine +
Chromic acid, ≤ 50% + + Methyl benzoate + + Urea +
Chromosulfuric acid + Methyl butyl ether + + Xylene +
Copper sulfate + Methyl ethyl ketone + + Zinc chloride, ≤ 10% +
Cresol + Methyl formate + + Zinc sulfate, ≤ 10% +
Cumene (Isopropyl benzene) + + Methyl propyl ketone + +
The above recommendations reflect testing completed prior to publication. Always follow instructions in the operating manual of the * use ETFE/PTFE bottle adapter
instrument as well as the reagent manufacturer‘s specifications. In addition to these chemicals, a variety of organic and inorganic saline ** use PTFE seal for valve block
solutions (e.g., biological buffers), biological detergents and media for cell culture can be dispensed. Should you require information
on chemicals not listed, please feel free to contact BRAND. Status as of: 1014/13
www.brand.de 23
Dispensette® III / Organic
Ordering Data
Ordering Data
Liquid Handling
Items supplied:
Each Dispensette® bottle-top dispenser has DE-M marking and is supplied with perfor-
mance certificate, discharge tube, telescoping filling tube, SafetyPrime™ recirculation valve
(optional), mounting tool and adapters of polypropylene:
Dispensette® Adapter for Filling tube
nominal volume, ml bottle thread length, mm
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-5. DE-M marking.
A = Accuracy, CV = Coefficient of variation
24 info@brand.de
Dispensette® III / Organic
Ordering Data
Liquid Handling
Dispensette ® Organic, Digital · Easy Calibration
Capacity Subdivision A* ≤ ± CV* ≤ without SafetyPrime™ with SafetyPrime™
ml ml % µl % µl recirculation valve recirculation valve
Cat. No. Cat. No.
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-5. DE-M marking.
A = Accuracy, CV = Coefficient of variation
www.brand.de 25
Dispensette® III / Organic
Accessories and Spare Parts
(Other spare parts and accessories can be found in the operating manual.)
n for Dispensette® III 0.5, 1, 2, 5, 10 fine tip 90 7079 15 for Dispensette® III 1-100 ml
n 7060 80
5, 10 standard 90 7079 16 for Dispensette® III 0.5 ml
n 7060 81
25, 50, 100 standard 120 7079 17 for Dispensette® Organic
n 7060 90
25, 50, 100 fine tip 120 7079 18
for Dispensette® Organic
n 0.5, 1, 2, 5, 10 fine tip 90 7079 35
5, 10 standard 90 7079 36
25, 50, 100 standard 120 7079 37 Discharge tube with
25, 50, 100 fine tip 120 7079 38 Luer-Lock attachment
for micro filter
With integrated valve.
Pack of 1.
Cat. No. 7079 28*
Bottle adapters * not suitable for HF and Peroxide
For Dispensette®, Titrette®,
seripettor® and QuikSip™.
PP or ETFE. Adapters of
ETFE offer higher chemical
resistance. Pack of 1.
GL 32 GL 25 ETFE 7043 75
GL 32 GL 28 / S 28 ETFE 7043 78
GL 32 GL 30 ETFE 7043 80
GL 32 GL 45 ETFE 7043 95
GL 45 GL 32 ETFE 7043 98
GL 45 GL 38 ETFE 7043 99
Threaded bottles, coated
GL 45 S* 40 PTFE 7043 91
and uncoated, see page 299.
GL 32 NS 19/26 PP 7044 19
GL 32 NS 24/29 PP 7044 24
GL 32 NS 29/32 PP 7044 29
* buttress thread
26 info@brand.de
Dispensette® III / Organic
Accessories and Spare Parts
Liquid Handling
Telescoping filling tubes Flexible discharge tubing
FEP. Adjusts to various PTFE tube, coiled, length
bottle heights. approx. 800 mm, with safety
Pack of 1. handle. Pack of 1.
Nominal volume Outer Ø Length Cat. No. Nominal volume Discharge tube Cat. No.
ml mm mm ml Outer Ø mm Inner Ø mm
for Dispensette® III, Dispensette® Organic 0.5, 1, 2, 5, 10 6697 for Dispensette® III, Dispensette® Organic 0.5, 1, 2, 5, 10 6637
for Dispensette® III, Dispensette® Organic 25, 50, 100 6698 for Dispensette® III, Dispensette® Organic 25, 50, 100 6638
Seals
PTFE. Spare seals for
Seal for valve block
discharge tube, SafetyPrime™ PTFE. For highly volatile
and filling valve. reagents.
Pack of 5 each type. Pack of 1.
Cat. No. 6696 Cat. No. 7044 86
www.brand.de 27
Dispensette® III / Organic
Remote Dispensing System
28 info@brand.de
Dispensette® TA
Liquid Handling
NEW!
Dispensette ® TA
Trace Analysis
The Dispensette® TA provides outstand-
ing performance for precise volume
dispensing of high-purity media for
trace analysis. Also suitable for HF with
platinum-iridium valve spring.
www.brand.de 29
Dispensette® TA
A Closer Look...
Liquid Handling
Piston seat
Dispensing cartridge
with serial number Volume adjustment
Safety ring
SafetyPrime™
recirculation valve
Discharge tube
Valve block
Screw cap
Recirculation tube
Replaceable dispensing
cartridge supplied with
certificate
30 info@brand.de
Dispensette® TA
Liquid Handling
General features of the Dispensette® TA
bottle-top dispenser
n Plastics in contact with media consist of high-purity
materials such as PTFE, ETFE, FEP, and PFA.
The purest sapphire is used for replaceable valves.
Depending on the application, platinum-iridium or
tantalum are available as spring materials.
n A field-tested cleaning process before use in trace
analysis is described in the operating manual.
n If contamination of the bottle contents must be
avoided, we recommend using the device without
the SafetyPrime™ recirculation valve.
Replaceable dispensing Serial dispensing n The 45 mm standard thread plus the included
cartridge adapters fit most common lab bottles.
If the piston seal is worn after For easy serial dispensing, n The valve block can be rotated 360° so that the
an extended period of use, the an optional flexible discharge bottle label always faces the user for safety.
entire dispensing cartridge tube with textured safety
can easily be replaced without handle (not approved for HF) n Telescoping filling tube adjusts to different bottle
tools by the user. The car- permits fast and precise dis- sizes.
tridge is fully adjusted at the pensing, even into narrow test n Easy disassembly for replacement of the dispensing
factory, and delivered with a tubes. Full functionality of the cartridge.
performance certificate. No SafetyPrime™ recirculation
calibration is required after valve and the safety discharge
replacement. system is maintained after
installation.
Various fluoroplastics (ETFE, Vapor pressure max. 600 mbar Acetic acid + +
FEP, PFA, PTFE), Al2O3 viscosity max. 500 mm2/s Ammonia solution + +
sapphire and platinum-iridium temperature max. 40 °C
or tantalum, depending on density max. 3.8 g/cm3 Bromine + +
design. Hydrochloric acid + +
Hydrofluoric acid* + –
Hydrogen peroxide – +
Nitric acid + +
Perchloric acid + +
Phosphoric acid + +
Sulfuric acid + +
Water + +
The above recommendations reflect testing completed prior to publication. Always follow instruc-
tions in the operating manual of the instrument as well as the reagent manufacturer‘s specifica-
tions. Should you require information on chemicals not listed, please feel free to contact BRAND.
Status as of: 0713/2
www.brand.de 31
Dispensette® TA
Ordering Data
Ordering Data
Liquid Handling
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-5. DE-M marking.
A = Accuracy, CV = Coefficient of variation
Bottle Stand
Flexible discharge tubing
PP. Full plastic construction –
PTFE, coiled, length 800 mm,
suitable for use in aggressive
with safety handle. Must not be
environment (e.g., acid fumes
used with HF! Pack of 1.
in the hood). Support rod
Nominal volume Valve spring Cat. No. 325 mm, base plate 220 x
ml 160 mm, weight 1130 g.
Pack of 1.
10 Platinum-iridium 7079 45
10 Tantalum 7079 46 Cat. No. 7042 75
32 info@brand.de
seripettor ®
seripettor ® pro
seripettor ®
seripettor® and seripettor® pro are innovative
bottle-top dispensers from BRAND with a
special design. They provide a cost-effective
alternative to high-end dispensers for routine Bottle-top Dispenser
laboratory use.
seripettor® · seripettor® pro
Models
Liquid Handling
– Alkaline solutions The seripettor® pro bottle-top dispenser extends the operating
The components of the instrument are compatible range to include the dispensing of
with alkaline solutions, such as NaOH, KOH and – acids such as concentrated HCl
ammonia. – polar solvents such as acetone
– UV-sensitive reagents
– Polar solvents
e.g., polar solvents such as ethanol, methanol, Information on use with your specific media can be found in the
acetylacetone, etc. guidelines for dispenser selection on page 36, or ask BRAND.
34 info@brand.de
seripettor® · seripettor® pro
A Closer Look...
Liquid Handling
The design of the seripettor® bottle-top dispenser makes
it possible for the user to replace any functional parts
when needed, quickly and easily without the use of tools.
Cleaning and maintenance work is reduced to a minimum.
Pump assembly
with lifting spring
The automatic lifting action
of a spring pushes the piston
up. This fills the dispensing
cartridge.
Volume settings
Scalloped track allow for quick
and exact setting of desired
volume. The selected volume
Replaceable dispensing can be easily read.
cartridge
If the dispenser’s piston
seal becomes worn, it is
easy and inexpensive to
replace. One spare car-
tridge included free.
Valve block
Valve block (GL 45) and inclu-
ded adapters (GL 32 and S 40)
match the threads of the most
common reagent bottles.
(See page 26 for an overview
Cap for discharge tube of available bottle adapters.)
Stopper cap or screw
cap (depending on
the model).
www.brand.de 35
seripettor® · seripettor® pro
Dispenser Selection Chart
The above recommendations reflect testing completed prior to publication. Always follow instructions in the operating manual of the
instrument as well as the reagent manufacturer‘s specifications. In addition to these chemicals, a variety of organic and inorganic saline
solutions (e.g., biological buffers), biological detergents and media for cell culture can be dispensed. Should you require information
on chemicals not listed, please feel free to contact BRAND. Status as of: 0114/9
Note! seripettor® and seripettor® pro are not suitable for HF.
For dispensing HF, we recommend the use of the
Dispensette® TA bottle-top dispenser with platinum-
iridium valve spring (Cat. No. 4740 041, page 32).
36 info@brand.de
seripettor® · seripettor® pro
Ordering Data
Ordering Data
Liquid Handling
seripettor ®
Items supplied:
Each seripettor® bottle-top dispenser is supplied with discharge tube, filling tube,
spare dispensing cartridge and PP adapters (GL 45/32 and GL 45/S40).
Volume Subdivision A* ≤ ± CV* ≤ Cat. No.
ml ml % µl % µl
seripettor ® pro
Items supplied:
Each seripettor® pro bottle-top dispenser is supplied with discharge tube, filling tube,
spare dispensing cartridge, mounting tool and PP adapters (GL 45/32 and GL 45/S40).
Volume Subdivision A* ≤ ± CV* ≤ Cat. No.
ml ml % µl % µl
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. A = Accuracy, CV = Coefficient of variation
www.brand.de 37
seripettor® · seripettor® pro
Accessories and Spare Parts
Liquid Handling
(1)
(2)
Pump assembly Discharge tube Filling valve (1) Adapter for discharge
seripettor ® pro seripettor ® pro seripettor ® pro tube seripettor ® pro
PPO. PEI (UV protection). With integrated valve Filling valve with seal. PP. With seal.
Hastaloy (stainless) lifting with seal. Pack of 1. Pack of 1.
spring. Pack of 1.
Description Cat. No. Cat. No. 6208
Pack of 1.
Description Cat. No.
6697
Description Cat. No. 2 + 10 ml
(2)Adapter for filling valve
2 ml 7079 15 25 ml 6698
2 ml 7045 51 10 ml
seripettor ® pro
7079 16 Filling valves are ordered sepa-
10 ml 7045 48 25 ml 7079 18 rately. PP. With seal.
25 ml 7045 49 Discharge tube adapters are Pack of 1.
ordered separately.
Cat. No. 6707
Length Pack of Cat. No. Nominal volume Outer Ø Length Cat. No.
mm ml mm mm
38 info@brand.de
Titrette® – the first bottle-top burette to sat-
isfy Class A error limits for glass burettes!
It is also remarkable for its easy dropwise ti-
tration, compact design, ultra-high precision,
replaceable piston/cylinder assembly and
Titrette ®
an optional PC interface. With the Titrette®
bottle-top burette, you can titrate quickly and
reliably with the highest precision, even in
close quarters, with no power hookup need- Bottle-top Burette
ed – in the lab, in production, or in the field.
Titrette®
A Closer Look...
Liquid Handling
PC interface
(optional)
Digital display
Replaceable batteries
Hand wheels
Titration and
recirculating valve
Valve block
Freely rotating
Titrating tube (Bottle thread GL 45)
horizontally and
vertically adjusta-
ble, with integrated
discharge valve
Recirculating tube
40 info@brand.de
Titrette®
Liquid Handling
Light-weight and compact
The compact and lightweight
design ensures good stabil-
ity. The titrating tube can
be adjusted horizontally and
vertically. This provides flex-
ibility when positioning the
instrument, e.g., when using
a magnetic stirrer or different
bottle sizes.
Useful extras
The instrument is equipped with four helpful additional electronic
functions:
www.brand.de 41
Titrette®
PC interface (optional)
Liquid Handling
When the instrument is properly handled, dispensed liquid will only come into contact
with the following chemically resistant materials: borosilicate glass, Al2O3, ETFE,
PFA, FEP, PTFE, platinum-iridium; PP (screw cap).
42 info@brand.de
Titrette®
Comparison
Liquid Handling
of error limits
Titrette® Bottle-top burettes according Glass burettes Class A acc. to
bottle-top burette to DIN EN ISO 8655-3 DIN EN ISO 385 and ASTM
NEW!
5 0.20 10 0.10 5 0.6 30 0.2 10 20
1 1.00 10 0.50 5 3 30 1 10 20
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) The titration volume is displayed in steps of 1 μl at instruments with
indicated on the instrument, obtained with instrument and distilled water at equilibrium with am- 10 ml and 25 ml size and in steps of 2 μl for 50 ml size instruments.
bient temperature at 20 °C, and with smooth, steady operation. The error limits are well within For titration volumes above 20 ml the display will automatically switch
the limits of DIN EN ISO 8655-3. DE-M marking. A = Accuracy, CV = Coefficient of variation to steps of 10 μl.
** Error limit: EL = A + 2CV, according to DIN EN ISO 8655-6 Annex B
The error limits for Class A burettes according to DIN EN ISO 385 are met.
Ordering Data
Titrette ®
Items supplied:
Each Titrette® bottle-top burette has DE-M marking and is supplied with performance certi-
ficate, telescoping filling tube (170 - 330 mm), recirculation tube, 2 batteries (AAA/UM4/
LR03), 3 PP bottle adapters (GL 45/32, GL 45/S 40, GL 32/NS 29/32), 2 amber colored
light shield inspection windows.
* Additionally included: 2 m interface cable (Sub-D plug connector, 9-pin), one CD (driver software and open RS232
communication protocol). The CD also includes an example application in XLS-file format, as well as a special operat-
ing manual.
www.brand.de 43
Titrette®
Accessories and Spare Parts
(Other spare parts and accessories can be found in the operating manual.)
Titrating tube
Telescoping filling tubes
With screw cap and integrated
discharge and recirculation FEP.
valve. Pack of 1. Pack of 1.
for volume Cat. No. Length Cat. No.
ml mm
Filling valve
Inspection window
With olive-shaped nozzle and
sealing ring. 1 set colorless and 1 set am-
Pack of 1. ber colored (light shield).
Dispensing cylinder
Piston with valve block
Pack of 1. Pack of 1.
for volume Cat. No. for volume Cat. No.
ml ml
10 7075 31 10 7075 33
25 7075 30 25 7075 35*
50 7075 32 50 7075 37*
Bottle Stand
PP. Full plastic construction.
Support rod 325 mm,
Drying tube
base plate 220 x 160 mm,
weight 1130 g. Drying tube and seal, without
Pack of 1. drying agent. Pack of 1.
Cat. No. 7042 75 Cat. No. 7079 30
44 info@brand.de
BRAND offers the ideal pipette for every
hand:
n Transferpette® S with the central pipetting
button and one-handed volume setting
n Transferpette® with the pipetting key on
the side
n Transferpette® electronic with motor drive
BRAND has been developing and manu-
facturing single-channel and multichannel
Transferpette ®
piston-operated pipettes for more than 25
years. Special attention is always paid to op-
timum ergonomics and reduction of injuries
caused by prolonged strain (such as Repeti- Pipetting System
tive Strain Injury Syndrome, RSI).
Transferpette®
Pipetting System
Pipetting – A Routine
Liquid Handling
Laboratory Procedure
Pipetting is one of the most frequent tasks in the laboratory.
The right choice of pipette is critical to performing this repetitive
task accurately and strain-free.
ic
tron
elec
ic
tron
12
12
/-
elec
8/-1
-8/-
S -8
S
te ®
te ®
te ®-
te ®
te ®
te ®
pet
pet
pet
pet
pet
pet
sfer
sfer
sfer
sfer
sfer
sfer
Tran
Tran
Tran
Tran
Tran
Tran
46 info@brand.de
Transferpette® S
Transferpette® S -8/-12
Liquid Handling
Transferpette ® S
Single and Multichannel Pipettes
The performance standard among pi-
pettes with a central pipetting button:
Transferpette® S pipettes from BRAND.
Transferpette ® S
Solutions for
S cience
www.brand.de 47
Transferpette® S
Transferpette® S -8/-12
Models
Liquid Handling
Lightweight – robust – low force:
The piston-operated pipette Transferpette® S provides maximum
versatility and optimum quality over the entire volume range.
There are 10 single-channel pipettes with adjustable volume
and 8 single-channel pipettes with fixed volume available in the
0.1 μl to 10 ml range.
There are 5 different multichannel pipettes available in the 0.5 to
300 μl volume range.
Features
Transferpette ® S
n Large, central pipetting button
and separate ejection function
n True one-handed operation for Transferpette ® S -8
both right-and left-handers
n The Transferpette® S is complete-
ly autoclavable at 121 °C (2 bar),
acc. DIN EN 285.
n Volume-change protection
n 4-position volume display, always
clearly visible
n Transferpette® S features Easy
Calibration technique – readjust-
ment without special tools (please
see page 324 for further details).
Clearly visible external flag indi-
cates changes from factory set-
tings.
n Short stroke of only 12.5 mm to
reduce the risk of RSI (Repetitive
Strain Injury)
n Corrosion-resistant piston and
ejector
n Color-coded volume range
n Transferpette® S 0.1-1 µl offers
maximum precision for molecular
biology work, especially when
pipetting enzymes.
n UV resistant
n CE- - compliant
48 info@brand.de
Transferpette® S
A Closer Look...
Liquid Handling
A central pipetting button, one-handed operation, precise 4-position
volume settings and a volume display that can be easily read by both
right- and left-handed operators are only a few examples of the atten-
tion to detail in the design of the Transferpette® S pipette.
Pipetting button
The centrally mounted, large
pipetting button allows uniform
smooth movement of the piston.
Volume adjustment
True one-handed volume adjust-
Ejection button
ment for right- and left-handed
Ergonomically arranged, with operators – even with gloves.
color code according to volume range
Tip cone
Due to the optimized standard design,
pipette tips from BRAND and other
manufacturers' tips can be used.
www.brand.de 49
Transferpette® S
Ordering Data
Ordering Data
Liquid Handling
Items supplied:
Each Transferpette® S adjustable and fixed volume pipette has DE-M marking and
is supplied with performance certificate, shelf/rack mount and silicone oil.
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
** For use only with 2-200 µl pipette tips
50 info@brand.de
Transferpette® S -8/-12
A Closer Look...
Liquid Handling
Easy Handling – a particular advantage of the new multichannel pipette is the easy operation,
e.g., when performing serial pipetting of immunological assays, while making serial dilutions
or when filling 96-well plates for cell cultures.
Pipetting button
The centrally mounted, large
pipetting button allows uniform
smooth movement of the piston. Volume adjustment
True one-handed volume
adjustment for right- and left-
Ejection button
handed operators – even with
Ergonomically arranged, with gloves.
color code according to volume range
Easy Calibration Technique
Volume-change protection Changes from factory settings
A lock prevents unintended are externally visible!
volume adjustments. (For more information, please
see page 324.)
Manifold
Can be freely rotated by 360°
in either direction.
www.brand.de 51
Transferpette® S -8/-12
Ordering Data
Ordering Data
Liquid Handling
Items supplied:
Each Transferpette® S -8/-12 pipette has DE-M marking and is supplied with performance
certificate, 2 x TipBox, filled with pipette tips from BRAND, 1 shelf/rack mount, 1 reagent
reservoir, 1 mounting tool, silicone oil and 1 set of sealing rings made of FKM.
Transferpette® S -8
Capacity, µl Description A* CV* Subdivision Cat. No.
(color-coded) ≤±% ≤% µl
Transferpette® S -12
Capacity, µl Description A* CV* Subdivision Cat. No.
(color-coded) ≤±% ≤% µl
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
For 6 Transferpette® S or
Transferpette® S -8/-12 pipet- For Transferpette® S 1-10 ml
tes. Pack of 1. pipettes. Pack of 25.
Cat. No. 7048 05 Cat. No. 7046 53
52 info@brand.de
Transferpette®
Transferpette®-8/-12
Liquid Handling
Transferpette ®
Single and Multichannel Pipettes
The Transferpette® pipette from BRAND is
designed for routine lab and research ap-
plications in a shape that is adapted to the
anatomy of the human hand. The special
handle shape with the side pipetting key al-
lows the Transferpette® pipette to lie loosely
and lightly in your hand.
The Transferpette® models are particularly
well suited for prolonged pipetting, or for
anyone who is susceptible to RSI syndrome
due to repetitive laboratory procedures.
www.brand.de 53
Transferpette®
Transferpette®-8/-12
Models
Liquid Handling
Features
Transferpette ®
■ Side pipetting key relieves strain;
separate ejector function limits
errors.
Transferpette ® -12
■ Pipette shafts/manifold are en-
tirely autoclavable at 121 ºC
(2 bar), acc. DIN EN 285.
■ Transferpette® adjustable volume
models and Transferpette®-8/-12
feature Easy Calibration tech-
nique – readjustment without
special tools (please see page
324 for further details).
■ Corrosion-resistant piston and
ejector
■ Tip ejector caps with color code
according volume range.
■ Transferpette® 0.1-1 µl offers
maximum precision for molecular
biology work, especially when
pipetting enzymes.
■ A variety of pipette stands
for optimum storage of the
Transferpette® pipette
■ UV resistant
■ CE- - compliant
54 info@brand.de
Transferpette®
A Closer Look...
Liquid Handling
The Transferpette® pipette is designed to
the shape of the human hand for maximum
comfort.
Pipette shaft
The slim shaft (autoclavable at
121 ºC) makes it possible to pipette
Tip cone in the narrowest vessels without
The taper is designed to fit pipette removing the tip ejector.
tips from BRAND and most other
leading manufacturers tips.
www.brand.de 55
Transferpette®
Ordering Data
Ordering Data
Liquid Handling
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
56 info@brand.de
Transferpette®
Accessories
Accessories
Liquid Handling
(Other accessories and spare parts can be found in the operating manual.)
Bench-top rack
Incl. 1 or 2 adapters for
Transferpette® pipettes 2 ml
or 0.5-5 ml.
Pack of 1.
Description Cat. No.
Filter
For all Transferpette® single-
channel pipettes 0.5-5 ml.
Pack of 25.
Cat. No. 7046 52
www.brand.de 57
Transferpette®-8/-12
A Closer Look...
Liquid Handling
The effortless ease of operation and its unique ergonomic shape make working
with the Transferpette®-8/-12 piston-operated pipette so comfortable. The use
of high-quality materials makes it exceptionally light in weight, while special FKM
V-rings and the stepped shape of the ejector significantly reduce the ejection
force needed. Your hand remains relaxed and loose, even during a prolonged
pipetting operation.
Individual shafts
and seals can
easily be replaced
in the laboratory.
58 info@brand.de
Transferpette®-8/-12
Ordering Data
Ordering Data
Liquid Handling
Items supplied:
Each Transferpette®-8/-12 pipette has DE-M marking and is supplied with performance
certificate, 2 x TipBox, filled with pipette tips from BRAND, 1 stand, silicone oil and
1 set of sealing rings made of FKM.
Transferpette®-8
Capacity, µl A* ≤ ± CV* ≤ Subdivision Cat. No.
(color-coded) % µl % µl µl
Transferpette®-12
Capacity, µl A* ≤ ± CV* ≤ Subdivision Cat. No.
(color-coded) % µl % µl µl
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
Accessories
(Other accessories and spare parts can be found in the operating manual.)
Individual stand
For all Transferpette® multi-
Reagent reservoir,
channel pipettes.
PP, non-sterile or sterile,
Pack of 1.
please see page 67.
Cat. No. 7034 40
www.brand.de 59
Transferpette® electronic
Transferpette®-8/-12 electronic
Liquid Handling
Transferpette ® electronic
Single and Multichannel Pipettes
The Transferpette® electronic piston-
operated pipette combines the widely rec-
ognized features of BRAND mechanical
pipettes with the advantages of electronic
apparatus.
Comfortable design, balanced weight distri-
bution, intuitive software and user-friendly
technical documentation were the key
objectives in developing the Transferpette®
electronic pipette.
TUV Rhineland/Berlin-Brandenburg has
confirmed the design as ergonomically sound
and easy to use after rigorous field testing.
It was the first pipette ever granted such
recognition as a comprehensive, ergonomic
concept.
60 info@brand.de
Transferpette® electronic
Transferpette®-8/-12 electronic
Models
Liquid Handling
Ergonomics – approved and certified.
Transferpette® electronic single channel pipette is available
in 5 different models: 0.5-10 µl, 2-20 µl, 20-200 µl, 100-
1000 µl and 0.5-5 ml.
The Transferpette®-8/-12 electronic multichannel pipette is
available in 5 different models: 0.5-10 µl, 1-20 µl, 5-100 µl,
10-200 µl and 15-300 µl.
Transferpette ® Features
electronic Ergonomic
– functional, ergonomic housing
design
Transferpette ® -8 – individually adjustable finger rest
electronic Easy operation
– intuitive menu structure
– comprehensively illustrated user
manual
Innovative
Significantly reduced tip attach-
ment and ejection forces using
universal tips
Resistant
Corrosion-resistant piston and
ejector
Five convenient programs
(Please see page 62 for details)
– Pipetting
– Reverse pipetting
– Mixing
– GEL-Electrophoresis
– Dispensing
Ready for use
– 4000 pipetting cycles with each
battery charge
– battery refresh function
– even during recharging
■ CE- - compliant
www.brand.de 61
Transferpette® electronic
Transferpette®-8/-12 electronic
Functions
Liquid Handling
The Pipetting
(PIP Mode)
Programs The 'standard' program.
The set volume is aspirated by the pipette,
and then discharged.
Mixing of Samples
(PIPmix Mode)
Program for mixing of liquids. The sample is
repeatedly aspirated and discharged, and the
number of mixing cycles is displayed.
Reverse Pipetting
(revPIP Mode)
Program specially designed for the pipetting
of liquids with a high viscosity, high vapor
pressure or foamy media.
Dispensing
(DISP Mode)
A program for the dispensing of liquids in a series
of equal aliquots. A volume that has been aspirated
is dispensed in steps.
Battery-Refresh
(batt Mode)
Regeneration function for increased performance and
extending the service life of the batteries. The world’s
first microliter pipette with this function.
* The GEL mode is not included in the 1000 μl and 5000 μl single channel pipettes
because these volumes are rarely used in electrophoresis.
** Patented
62 info@brand.de
Transferpette® electronic
A Closer Look...
Liquid Handling
The single channel pipette Transferpette® electronic was
the first microliter pipette worldwide to be recog-
nized with the 'Ergonomics Approved' certificate from the
Technical Control Board Rhineland/Berlin-Brandenburg!
Independent user tests confirm the ergonomics and the
operating ease of the product and system! A user accep-
tance rating of 1.54 is an outstanding result.
You can obtain information about the Transferpette®
electronic pipette at www.tuv.com, ID No. 0011105500.
Individually adjustable
finger rest
Color indicates
volume range
www.brand.de 63
Transferpette® electronic
Ordering Data
Ordering Data
Liquid Handling
Transferpette® electronic
Items supplied:
Each Transferpette® electronic pipette has DE-M marking and is supplied with performance
certificate, battery, AC adapter, silicone oil.
Capacity, µl Subdiv. A* ≤ ± CV* ≤ With AC adapter Cat. No.
(color-coded) µl % µl % µl for
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
64 info@brand.de
Transferpette®-8/-12 electronic
A Closer Look...
Liquid Handling
The optimal position of the thumb relative to the functional
elements of the pipette is the starting point for a relaxed
grip. Avoiding RSI is the key.
The optimum design, the layout of the controls, and the
adjustable finger rest provide a Transferpette®-8/-12
electronic multichannel pipette that fits the hand like a
glove. Perfect for right-handers and left-handers alike!
The Transferpette®-8/-12 electronic pipette was the
world‘s first electronic multichannel pipette to received
the Ergonomics Certificate. The User Acceptance
Charging connector jack
Rating of 1.55 is unrivaled anywhere!
Individually adjustable
finger rest
www.brand.de 65
Transferpette®-8 electronic
Ordering Data
Ordering Data
Liquid Handling
Items supplied:
Each Transferpette®-8/-12 electronic pipette has DE-M marking and is supplied with
performance certificate, battery, AC adapter, device stand, TipBox, refill unit, reagent
reservoir, mounting tool, silicone oil and 1 set of sealing rings made of FKM.
Transferpette ® -8 electronic
Capacity, µl Subdivision A* ≤ ± CV* ≤ With AC adapter Cat. No.
(color-coded) µl % µl % µl for
* Calibrated to deliver (TD, Ex). Erro r limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
66 info@brand.de
Transferpette®-12 electronic
Ordering Data
Liquid Handling
Transferpette ® -12 electronic
Capacity, µl Subdivision A* ≤ ± CV* ≤ With AC adapter Cat. No.
(color-coded) µl % µl % µl for
* Calibrated to deliver (TD, Ex). Error limits according to the nominal capacity (= maximum volume) indicated on the
instrument, obtained with instrument and distilled water at equilibrium with ambient temperature at 20 °C, and with
smooth, steady operation. The error limits are well within the limits of DIN EN ISO 8655-2. DE-M marking.
A = Accuracy, CV = Coefficient of variation
Accessories
(Other accessories and spare parts can be found
in the operating manual.)
Reagent reservoir
PP, high clarity.
Capacity 60 ml.
Autoclavable (121 °C).
Sterile, without lid.
Non-sterile, with lid. Packed individually. Sterile, without lid.
Pack of 10. Pack of 100. 5 per bag, pack of 200.
Cat. No. 7034 59 Cat. No. 7034 11 Cat. No. 7034 09
www.brand.de 67
Pipette Tips and Filter Tips
Liquid Handling
Pipette Tips
and Filter Tips
Standard
from page 74
Cadmium-free pigments
68 info@brand.de
Pipette Tips and Filter Tips
Liquid Handling
Pipette tips and filter tips are the most fre- For the manufacture of pipette tips and filter tips, BRAND ex-
quently used disposables in the laboratory. clusively uses raw materials that are free from the additives
As processing methods have become in- di(2-hydroxyethyl)methyldodecylammonium (DiHEMDA) and
creasingly sensitive, the requirements for 9-octadecenamide (oleamide). Both of these additives are fre-
these disposable items have changed signifi- quently found in PP granules, and can interfere with biological
cantly over the years. Starting with the raw tests, leading to spurious results. Only highly polished tooling
materials, here PP, to the tools used and the equipment is used, so that no parting agents or demolding aids
quality tests carried out, a lot of parameters are needed.
need to be considered to meet the highest
standards both in research and standardized
applications.
Cleanroom technique
BRAND disposable items for the life sci- the actual parameters can immediately be final product by batch, guarantees
ences are produced using the most advanced checked against the nominal values. Devia- the consistently high quality in the life
cleanroom techniques in one of the world's tions are detected immediately, and suitable science products from BRAND.
largest cleanrooms for laboratory disposable countermeasures can be taken before the For the production of disposable
items. limit values are exceeded. items, class 8, 7, and 5 manufac-
The ongoing cleanroom monitoring includes The high-precision control of environmental turing environments are available.
continuous measurements of air particulates, conditions provides a very high degree of Compliance of our class 8 cleanroom
positive air pressure, air exchange rate, stability in the corresponding parameters, with ISO 14 644-1 is validated and
room temperature, and the relative humid- especially the room temperature. This uni- certified by external, independent au-
ity, among other things. This ensures that formity, together with quality testing of the ditors.
www.brand.de 69
Pipette Tips and Filter Tips
0.1 - 1 µl
0.5 - 20 µl
0.5 - 10 µl
1 - 50 µl
1 - 20 µl
2 - 200 µl
2 - 20 µl
0.1 - 20 µl 0.5 - 20 µl 1 - 50 µl 2 - 200 µl
0.1 - 1 µl (Filter) 0.5 - 10 µl (Filter) 1 - 20 µl (Filter) 2 - 20 µl (Filter)
The nano-cap™ tip was spe- The slender design and 46 mm With a length of 50 mm, the tip A tested and proven thin-
cially developed for the nano- length allow pipetting in micro- is ideal for working down to the walled tip. Lighter in weight,
liter range and hence is ideal tubes and microplates without bottom of narrow containers. 50 mm long and can be used
for molecular biological ap- touching the walls. Graduation Graduation at 2.5, 10, 25 and for virtually all pipettes with
plications such as PCR. It is at 2 µl and 10 µl for quick 50 µl for quick volume check. yellow color code. Graduation
37 mm long, and features a volume check. The racked tips The racked tips are colorless at 20 µl and 100 µl for a quick
capillary rise that is even visible are colorless and placed into a and placed into a grey-colored volume check. Bulk tips are
to the naked eye at 0.1 µl. The grey-colored tip tray. tip tray. yellow colored. The racked tips
capillary part of the tip con- are colorless and placed into a
veniently fills gel wells for gel yellow-colored tip tray.
electrophoresis systems from
most manufacturers. Suitable
for pipettes up to 20 µl. The
racked tips are colorless and
placed into a grey-colored tip
tray.
70 info@brand.de
Pipette Tips and Filter Tips
Liquid Handling
NEW!
50 - 1000 µl
50 - 1000 µl
0.5 - 5 ml
1 - 10 ml
5 - 300 µl
5 - 100 µl
5 - 200 µl
5 - 300 µl 50 - 1000 µl
5 - 100/200 µl (Filter) 50 - 1000 µl (Filter)
The thin-walled tip is suitable Proven thin-walled tip for rou-
for reverse pipetting and plate tine laboratory and research
washing. It is 53 mm long and pipetting. Its length is 70 mm.
can also be used for pipettes Graduation at 250, 500 and
with yellow color code. It is par- 1000 µl for a quick volume
ticularly suited for working with check. Bulk tips are blue col-
multichannel pipettes. Gradua- ored. The racked tips are col-
tion at 50 and 100 µl for quick orless and placed into a blue-
volume check. The racked tips colored tip tray.
are colorless and placed into a
green-colored tip tray. 0.5 - 5 ml 1 - 10 ml
Particularly slender shape, at 156.5 mm length and ap-
160 mm length and approx. prox. 15 mm diameter! Ideal
9.6 mm diameter! This al- for working with the microliter
lows pipetting even from narrow pipette Transferpette® S 10 ml.
volume measuring equipment Compatible with Eppendorf®
such as volumetric flasks with systems.
NS 12/21. Suitable for mi-
croliter pipette Transferpette®
www.brand.de 71
Pipette Tips and Filter Tips
TipBox/TipRack System
Liquid Handling
No deflection during
tip loading
The tip-tray is fabri-
Dual function cated from especially
hinged and push-on lid rigid PP.
Simply rotate 180° to
change closure method.
Transparent window
For better all-round
visual inspection.
72 info@brand.de
Pipette Tips and Filter Tips
Liquid Handling
TipRack
Compared to the filled TipBoxes, the amount of waste with
the new refill units is reduced by over 20%. All racked pipette
tips and filter tips up to 1000 µl are free of DNA, RNases,
endotoxins and ATP (for detailed information see page 118).
TipRacks in BIO-CERT® quality are sterile according to ISO
11 137 and the AAMI guidelines, a SAL of 10-6 is obtained.
These racks are supplied with a transfer aid that enables
simple, contamination-free transfer into a previously sterilized
TipBox. All tip-trays are printed on one side with information
on the contents.
TipRacks
TipStack™
Transfer aid
Sterile handling
Press the long sides of the
transfer aid together, and
continue pressing them
together while withdrawing
TipStack™ the tip-tray.
A tip tower containing 5 filled tip-trays and a TipBox Ensure that the holding
constitute the new, space-saving refill system for straps for the transfer aid
20 µl, 200 µl and 1000 µl tips. are correctly positioned.
Tightly sealing spacers prevent the tips from getting
stuck together, and ensure them to be free from
DNA, RNases, endotoxins and ATP.
The sterile TipStacks (BIO-CERT® quality) are sup- Insert the filled tip-tray
plied with a transfer aid for contamination-free use perpendicularly from above
in a previously sterilized TipBox. into the previously sterilized
TipBox until it locks into
place.
n All components are recyclable
n Reduced amount of waste
n Sterilizable and reusable TipBox
n High purity of the pipette tips
and filter tips
Remove the transfer aid from
n Space-saving design
the mounting plate. Finished
– all without tip contact!
www.brand.de 73
Pipette Tips
sterile and non-sterile
Pipette Tips
Liquid Handling
1 - 50 µl Pipette tips, 1 - 50 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
up to 50 µl 7329 90
TipBox, with tip-tray, for 200 µl 7329 92
empty for 300 µl 7329 94
PP. Stackable. Pack of 1. for 1000 µl 7329 96
74 info@brand.de
Pipette Tips
sterile and non-sterile
Liquid Handling
Pipette tips, 5 - 300 µl
5 - 300 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
Pipette tips, 1 - 10 ml
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
www.brand.de 75
Filter Tips
sterile and non-sterile
Filter Tips
Liquid Handling
Non-self-sealing filter tips from BRAND have Since the filter does not swell as in the case
a PE filter that is free from chemical addi- of self-sealing filter tips, the sample can be
tives. Permeability is controlled by the combi- recovered from the filter by simply actuating
nation of pore size and filter length, so that no the pipette‘s blow-out function, or by cen-
aerosols can reach the pipette shaft. These trifugation if necessary. This is clearly an im-
filters function with consistent reliability. On portant advantage of non-self-sealing filters,
the other hand, liquids can pass very slow- especially when working with valuable sam-
ly should they accidentally contact the filter. ples. As an added advantage, the absence
of filter additives protects samples from con-
tamination.
Pipette tip
Filter tip
1 - 20 µl
Filter tips, 1 - 20 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
76 info@brand.de
Filter Tips
sterile and non-sterile
2 - 20 µl
Liquid Handling
Filter tips, 2 - 20 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
www.brand.de 77
ULR Pipette Tips
sterile and non-sterile
The surfaces of the Ultra Low Retention Ideal for biological samples that contain
tips are produced through a special physi- detergents such as TRITON™ X-100,
cochemical process. The homogeneous, SDS, Tween etc.
defect-free surface thus produced has ex-
No additives that can be leached out!
tremely low surface tension – over 50% less
No siliconization of the surface!
than PTFE (see table). This significantly re-
duces sample loss and provides substantially High chemical resistance. Ideal for wor-
higher reproducibility when working with king with solvents.
critical media.
The tips can be autoclaved at 121 °C
(2 bar) without damaging the material
properties.
Ultra Low Retention pipette tip
PTFE 19 mN/m
Silicone 21.5 mN/m
Untreated PP 30 mN/m
Water 72 mN/m
78 info@brand.de
ULR Pipette Tips
sterile and non-sterile
Liquid Handling
ULR pipette tips, 1 - 50 µl
1 - 50 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
TipBox TipStack™
www.brand.de 79
ULR Filter Tips
sterile and non-sterile
1 - 20 µl
ULR filter tips, 1 - 20 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
80 info@brand.de
ULR Filter Tips
sterile and non-sterile
Liquid Handling
ULR filter tips, 5 - 200 µl
5 - 200 µl
Quantity Pack of non-sterile sterile
Cat. No. Cat. No.
TipBox
Comparison chart
Standard and Ultra Low Retention pipette tips from BRAND
Volume 200 µl, compared with various media and subsequent photometric analysis and conversion.
4.5 4.58
0.2
4
3.5
0.15 0.15
3
% Residual quantity
% Residual quantity
2.5 2.5
2.37 2.31 2.37
0.1
2
1.84 1.8
1.5 1.4
1.2 1.3
0.05 0.05
1
0.5 0.5
0.1 0.023 0.04 0.06 0.04
BSA 1% TRITON™ SDS Isopropanol Methanol Food coloring n-Decane DMSO PCR master mix
X-100 60% 100% (green)
BRAND Ultra Low Retention pipette tips BRAND standard pipette tips
www.brand.de 81
Pipette Tips
Filter Tips
Note: Pipette shafts can be subject to modification. The fit depends on the manufacturer, pipette
type, serial number, and date of manufacture, among other things. We recommend checking the fit
of the tips using the free sample bag.
ic
tron
elec
ic
12
tron
12
12
/-
-8/-
elec
S -8
-8/-
S
te ®
te ®
te ®
te ®
te ®
te ®
pet
pet
pet
pet
pet
pet
sfer
sfer
sfer
sfer
sfer
sfer
Tran
Tran
Tran
Tran
Tran
Tran
The right Pipette Tip
Transferpette® single channel Transferpette® multichannel
Nominal volume Nominal volume
Volume range
Pipette tips
Filter tips
200 µl 3 )
1000 µl
10 µl 2 )
2)
100 µl
200 µl
250 µl
500 µl
100 µl
200 µl
300 µl
2.5 µl
20 µl
10 ml
10 µl
20 µl
25 µl
50 µl
10 µl
20 µl
25 µl
50 µl
2 ml
5 ml
1 µl
5 µl
0.1 - 20 µl 4 44 ) 44 ) 44 ) 4 4
0.5 - 20 µl 4 4 4 4 4
1 - 50 µl 44 ) 4 4 4 4
2 - 200 µl 1)
4 4 4 4 4 4 4 4 4 4 4 4
5 - 300 µl 4 4 4 4 4 4 4 4 4 4 4 4
50 - 1000 µl 1)
4 4 4 4
0.5 - 5 ml 4 4
1 - 10 ml 4
0.1 - 1 µl 4 44 ) 4
0.5 - 10 µl 4 4 4 4 4
1 - 20 µl 44 ) 4 4 4 4
2 - 20 µl 4 4 4 4 4 4 4 4 4 4 4
5 - 100 µl 4 4 4 4 4 4 4 4 4 4 4 4
5 - 200 µl 4 4 4 4 4 4
50 - 1000 µl 4 4
4 = Tip volume less than pipette's nominal volume
1)
Bulk tips are yellow or blue colored, racked tips are colorless in a yellow-colored or blue-colored tip tray
2)
Transferpette® with yellow color code
3)
Transferpette® with blue color code
4)
Calibration and possible adjustment needed
82 info@brand.de
The Transferpettor pipette is ideal for liquids
when air displacement pipettes just won't
work. Viscous, foaming, high vapor pres-
Transferpettor
sure: the Transferpettor pipette can handle
them all, with the precision and accuracy you
expect from a BRAND pipette. This is the
pipette for your most demanding pipetting Piston-operated pipette
operations.
Transferpettor
A Closer Look...
Liquid Handling
The Transferpettor pipette operates on the There is no need to discard tips after each
positive displacement principle. pipetting operation, since residual wetting
In contrast with air displacement pipettes, is negligible.
the piston is in direct contact with the aspi-
rated liquid. The movable, precision-fit pis- However, in cases where no carry-over can
ton always glides smoothly along the walls be tolerated, for example with infectious
of the capillaries and tips, right on through or radioactive media, a different BRAND
to the last drop that can be clearly observed Transferpette® model is recommended,
as it leaves the opening. such as an air displacement pipette with
a disposable tip for convenient operation
In this way, the results obtained are exactly
(page 45).
reproducible regardless of the pipetting rate
and environmental conditions.
84 info@brand.de
Transferpettor · Ordering Data
Application
Liquid Handling
Media which tend to foam Media with high vapor Highly viscous media and
pressure media with high density
– surfactant solutions
– alcohols, ether, hydro- – highly concentrated
carbons protein solutions, oils,
resins, fats
– glycerin, mercury,
sulfuric acid
Ordering Data
Transferpettor, Digital-adjustable
Capacity A* ≤ ± CV* ≤ Subdivision Color code Cat. No.
µl % µl % µl µl
Transferpettor, Fixed-volume
Capacity A* ≤ ± CV* ≤ Color code Cat. No.
µl % µl % µl
www.brand.de 85
Transferpettor
Accessories and Spare Parts
Seals, PTFE
DE-M marking.
Pack of 3, with mounting block.
For capacity Cat. No.
µl
Caps, glass
DE-M marking. 20, 25 7019 20
Pack of 100 (except 100/200 µl: pack of 50). 50 7019 22
100, 200 7019 24
For nominal Color code Cat. No.
volume, µl
Seals, PE
1, 2, 3, 4, 5 white 7019 00
DE-M marking.
10 orange 7019 02 Pack of 10.
20 black 7019 04
25 2 x white 7019 06 For capacity Cat. No.
µl
50 green 7019 08
100, 200 blue 7019 10
100 - 500 7028 64
200 - 1000 7028 66
1000 - 5000 7028 70
Caps, PP
2000 - 10000 7028 72
DE-M marking.
Pack of 10.
For capacity Color code Cat. No.
µl
1, 2, 5 – 7019 64 1, 2, 5 7019 28
10 – 7019 65 10 7019 30
20, 25 natural 7019 66 20, 25 7019 32
50 green 7019 67 50 7019 34
100, 200 blue 7019 68 100 7019 36
200 7019 38
86 info@brand.de
Serial pipetting can be quick and easy with
the HandyStep® S repetitive pipette. Ergo-
nomic and durable, the instrument, in con-
junction with PD-Tips from BRAND, gives you
as many as 49 repetitive dispensings from
HandyStep ® S
a single aspiration. The simple operation of
the HandyStep® S repetitive pipette, com-
bined with the positive displacement PD-Tips,
makes it ideal for versatile use in fields like Repetitive Pipette
microbiology, immunology and biochemistry.
HandyStep® S
A Closer Look...
Liquid Handling
Finger rest
Ergonomic finger loop to keep
the hand relaxed, important when
conducting long series.
Ejection key
Locking/filling lever
At the push of a button!
Ergonomic eject button Just one lever for locking the
for contact-free, safe piston and filling the PD-Tip.
ejection of contami-
nated dispenser
tips.
PD-Tip
In addition to PD-Tips from
BRAND, compatible dispenser
tips from other manufacturers
can also be used.
88 info@brand.de
HandyStep® S
Liquid Handling
n Increased chemical resistance
through innovative plastic materi-
als
n Easy tip mounting – PD-Tip is
now simply inserted from below
n Dispensed volume range from
2 μl to 5 ml
n Up to 49 dispensing steps
n Weighs only 108 g
Repetitive Dispenser tip n CE- -compliant
dispensing ejection
Steps
0.1 0.5 1 1.25 2.5 5 10 12.5 25 50
Volume (µl)
www.brand.de 89
HandyStep® S · Ordering Data
Ordering Data
Liquid Handling
HandyStep ® S
Items supplied:
Each HandyStep® S repetitive pipette has
DE-M marking, serial number, performance
certificate, shelf/rack mount, 3 PD-Tips:
0.1 ml, 1 ml and 10 ml.
Pack of 1. Note! BRAND also offers calibration
service at the factory lab (for more
Cat. No. 7051 10 information, please see page 326).
Accessory
Shelf/rack mount
for HandyStep® S. Can be
fitted to the bench-top rack of
Transferpette® S (page 52).
Pack of 1.
Cat. No. 7051 30
90 info@brand.de
The HandyStep® electronic repetitive pipette
was designed to provide effortless pipetting
for repetitive serial dispensing. Reduced
operating forces, intuitive menu and easy-
HandyStep ® electronic
to-read display further simplify repetitive Repetitive Pipette
pipetting.
HandyStep® electronic
A Closer Look...
Liquid Handling
Function
Number of steps
Dispensing
Battery status
Speed
Selection key 1
Selection key 2
92 info@brand.de
HandyStep® electronic
Liquid Handling
Dispensing (DISP) Automatic Dispensing Pipetting (PIP)
the standard mode (AUTO-DISP)
Single aspiration/dispense posi-
A predefined volume is dispensed The instrument uses its patented tive displacement function. Ideal for
repeatedly. learning function to calculate the pipetting viscous or volatile fluids.
average time interval between your
first three dispensing steps, and
automatically continues to work at
this rhythm. No need to calculate and
enter time intervals manually!
0.1 ml 1 µl - 100 µl 1 µl - 100 µl 0.1 µl 1.0 1.0 1.6 8.0 0.5 1.0 2.0 12.0
5 µl - 100 µl 0.1 µl
0.5 ml 5 µl - 500 µl 0.9 0.9 1.0 5.0 0.3 0.6 1.0 5.0
100 µl - 500 µl 1 µl
1.0 ml 10 µl - 1 ml 10 µl - 1 ml 1 µl 0.6 0.9 1.0 5.0 0.3 0.5 0.8 4.0
12.5 µl - 100 µl 0.5 µl
1.25 ml 12.5 µl - 1250 µl 100 µl - 1000 µl 1 µl 0.6 0.6 0.9 5.0 0.2 0.5 0.7 4.0
1 ml - 1.25 ml 10 µl
25 µl - 1000 µl 1 µl
2.5 ml 25 µl - 2500 µl 0.5 0.6 0.7 3.5 0.15 0.3 0.6 3.0
1 ml - 2.5 ml 10 µl
50 µl - 1000 µl 1 µl
5.0 ml 50 µl - 5000 µl 0.5 0.5 0.7 3.5 0.15 0.4 0.7 3.0
1 ml - 5 ml 10 µl
10.0 ml 100 µl - 10 ml 100 µl - 10 ml 10 µl 0.4 0.5 0.7 3.5 0.15 0.5 0.8 4.0
125 µl - 1000 µl 5 µl
12.5 ml 125 µl - 12.5 ml 1 ml - 10 ml 10 µl 0.5 0.5 0.8 3.5 0.15 0.6 1.4 6.5
10 ml - 12.5 ml 100 µl
250 µl - 10 ml 10 µl
25.0 ml 250 µl - 25 ml 0.5 0.5 0.6 3.0 0.15 0.3 1.0 6.0
10 ml - 25 ml 100 µl
500 µl - 10 ml 10 µl
50.0 ml 500 µl - 50 ml 0.5 0.5 0.5 3.0 0.15 0.4 1.2 9.0
10 ml - 50 ml 100 µl
* Error limits refer to the nominal volumes and partial volumes relative to the PD-Tip, obtained with instrument and distilled water at equilibrium with ambient temperature at
20 °C, and with smooth operation. The error limits defined in ISO 8655 are not exceeded. A = Accuracy, CV = Coefficient of variation
The nominal volume is the maximum volume printed on the PD-Tip.
www.brand.de 93
HandyStep® electronic
Ordering Data
Ordering Data
Liquid Handling
HandyStep® electronic
Items supplied:
Each HandyStep® electronic has DE-M
marking and is supplied with performance
certificate, NiMH battery pack, charging
dock and AC adapter. One each PD-Tip
size 0.5 ml, 1.25 ml, 2.5 ml, 5 ml and
12.5 ml.
AC adapter Cat. No.
Accessories
AC adapter for charging dock
Pack of 1.
Description Cat. No.
Charging dock
Europe (continental) (230 V/50 Hz) 7050 50 NiMH Battery Pack
Without AC adapter.
UK/Ireland (230 V/50 Hz) 7050 51
Pack of 1. Pack of 1.
USA/Japan (110 V/50-60 Hz) 7050 52
Australia (240 V/50 Hz) 7050 53 Cat. No. 7050 20 Cat. No. 7050 25
94 info@brand.de
PD-Tips
Liquid Handling
PD-Tips
Precision Dispenser Tips
The PD-Tips with patented size encoding are the ideal
system component for the repetitive pipettes HandyStep®
electronic (with automatic tip size recognition) and
HandyStep® S from BRAND. The PD-Tips comply with
ISO 8655 requirements and come with a batch certificate.
CE-marked according to IVD-Directive 98/79 EC. PD-Tips
are available either non-sterile or sterile/endotoxin-free
(individually wrapped), as well as in BIO-CERT® quality
(see page 118 for detailed information).
0.1 ml 1.0 µl - 100 µl 1.0 1.0 1.6 8.0 0.5 1.0 2.0 12.0
0.5 ml 5.0 µl - 500 µl 0.9 0.9 1.0 5.0 0.3 0.6 1.0 5.0
1.0 ml 10.0 µl - 1 ml 0.6 0.9 1.0 5.0 0.3 0.5 0.8 4.0
1.25 ml 12.5 µl - 1250 µl 0.6 0.6 0.9 5.0 0.2 0.5 0.7 4.0
2.5 ml 25.0 µl - 2500 µl 0.5 0.6 0.7 3.5 0.15 0.3 0.6 3.0
5.0 ml 50.0 µl - 5000 µl 0.5 0.5 0.7 3.5 0.15 0.4 0.7 3.0
10.0 ml 100 µl - 10 ml 0.4 0.5 0.7 3.5 0.15 0.5 0.8 4.0
12.5 ml 125 µl - 12.5 ml 0.5 0.5 0.8 3.5 0.15 0.6 1.4 6.5
25.0 ml 250 µl - 25 ml 0.5 0.5 0.6 3.0 0.15 0.3 1.0 6.0
50.0 ml 500 µl - 50 ml 0.5 0.5 0.5 3.0 0.15 0.4 1.2 9.0
* Error limits refer to the nominal volumes and partial volumes relative to the PD-Tip. obtained with instrument and distilled
water at equilibrium with ambient temperature at 20 °C. and with smooth operation. The error limits defined in ISO 8655
are not exceeded. A = Accuracy. CV = Coefficient of variation
The nominal volume is the maximum volume printed on the PD-Tip.
www.brand.de 95
PD-Tips · Ordering information
Liquid Handling
PD-Tips, non-sterile
Precision Dispenser Tips
Capacity Pack Cat. No.
ml of
PD-Tip Set (20 PD tips each in sizes of 0.5, 1, 1.25, 2.5, 5, 10 and 12.5 ml) 7023 68
* incl. 1 adapter
PD-Tips, sterile
Precision Dispenser Tips, individually wrapped
Capacity Pack sterile/endotoxin-free BIO-CERT®
ml of Cat. No. Cat. No.
non-sterile 7023 98
BIO-CERT® 7023 99
25 ml 50 ml
96 info@brand.de
Pipetting aids from BRAND excel by their
comfortable grips, superior control, light
weight and rugged reliability:
■ accu-jet® pro
■ macro
■ micro
Pipetting Aids
■ micro-classic
accu-jet® pro
A Closer Look...
Liquid Handling
Specifications
Weight: 190 g
Operating and charging temperature:
+10 °C to + 35 °C
Recharging-indicator (LED) Pipetting speed:
50 ml in less than 10 seconds
Battery compartment cover For glass and plastic pipettes
from 0.1 to 200 ml
Approx. eight hours of continuous
Mode selection
pipetting (with a 10 ml pipette) wit-
(gravity-delivery/blow-out)
hout recharging
Variable motor speed Battery pack:
NiMH battery 2.4 V / 700 mAh
Pipetting
buttons
98 info@brand.de
accu-jet® pro
Liquid Handling
Single-handed operation
All with one hand: select the delivery
mode (gravity-delivery/blow-out) and
adjust the motor speed range with your
thumb; use variable button pressure for
fine control of filling and delivery speed.
Advanced charging
The intelligent battery charger prevents
overcharging of the NiMH battery.
It effectively reduces the lazy-battery-
effect (shortened operating time due to
premature recharging).
A flashing LED indicates when the stor-
age battery needs charging. Charging
time is 4 hours. After that, the charger
automatically switches to a pulsed, long-
term charging mode. The pipette control-
ler is always ready for action, even while
being charged.
Tidy storage
Keep your instrument within reach by
placing it inverted on your lab bench. Or
store it in the space saving wall support.
Four colors
Select from four colors to individualize
your pipette controller.
Ordering Data
accu-jet ® pro
Items supplied:
Each pipette controller is supplied with nickel-metal hydride battery, 2 battery compartment
covers, wall support, AC adapter (100 - 240 V; 50 / 60 Hz) and 2 spare membrane filters
0.2 µm, sterile.
Spare Parts
Color dark blue magenta green royal blue (Other spare parts and accessories can be
accu-jet® pro Cat. No. Cat. No. Cat. No. Cat. No. found in the operating manual.)
www.brand.de 99
macro
Ordering Data
BLAUBRAND ®
Pipetting Package
Items supplied:
n 1 macro pipette controller, gray
n 6 BLAUBRAND® graduated pipettes,
type 2
3 pipettes 5 ml and 10 ml each, macro Pipette Controller
class AS, DE-M marking, Items supplied:
with batch certificate
Each pipette controller is supplied
n Useful product information with a 3 µm spare membrane filter.
BLAUBRAND® volumetric instruments,
handling of pipettes Color Cat. No.
100 info@brand.de
micro · micro-classic
Liquid Handling
The micro pipette controller is an indispens-
able accessory for sampling with disposable
micropipettes with ring mark and many micro Pipette Controller
small volume pipettes up to 1 ml (e.g.,
blood diluting and blood sugar pipettes) Pack of 1.
with aspiration end-Ø max. 5 mm. Cat. No. 258 00
The micro reduces the hazards of infection
and is autoclavable at 121 °C.
The integrated ejection device allows the Spare suction system
disposal of contaminated pipettes up to Pack of 3.
50 µl without touching them, thus helping
to prevent the transmission of dangerous Cat. No. 258 05
viruses such as hepatitis B or HIV.
The micro is extremely light and very
convenient.
A high-performance team:
micro Pipette Controller and
BLAUBRAND® Disposable
Micropipettes, page 251.
www.brand.de 101
Pipette fillers
Pipette fillers
Liquid Handling
Simple pipetting aids made of natural rubber for one-mark and graduated pipettes.
Control of the functions by squeezing the appropriate valves between thumb and
forefinger.
Pipette filler
Standard model, for pipettes up to 10 ml.
Pipette filler with 3 valves.
Valve A: Release air
Valve S: Filling
Valve E: Delivery
Pack of 1.
Cat. No. 253 00
Pipette filler
Universal model, for pipettes up to 100 ml.
Pipette filler with 3 valves.
Valve A: Release air
Valve S: Filling
Valve E: Delivery
Pack of 1.
Cat. No. 253 15
Pipette filler
Flip model, for pipettes up to 100 ml.
Pipette filler with 2 valves.
Release air through an automatic valve.
Valve ↑: Filling
Valve ↓: Delivery
Pack of 1.
Cat. No. 254 00
102 info@brand.de
The QuikSip™ bottle-top aspirator from
BRAND is designed for safe and fast aspi-
ration of common laboratory liquids used in
biology, food chemistry and medicine.
QuikSip ™
Adapter
■ and suction tube of the
cell-culture™-unit are autoclavable at
121 °C (2 bar), acc. DIN EN 285.
Dispensing cartridge and pump unit are BT-Aspirator
not autoclavable.
QuikSip™ · Ordering Data
Ordering Data
Liquid Handling
QuikSip ™ BT-Aspirator
Spare parts Quik-Sip ™
Items supplied:
1 QuikSip™ BT-Aspirator, Description Cat. No.
1 cell-culture™-unit incl. suction tube and
3 adapters, spare dispensing cartridge Seals for QuikSip™ (Pack of 5) 6788
and 2 PP adapters (GL 45/32 and Filling tube (PP)
7045 75
GL 45/S 40). with filling valve (PP/EPDM)
Discharge valve (PP/EPDM)
7045 80
Cat. No. 4723 150 with seal (EPDM)
Accessories
8-channel manifold Dispensing cartridge
and Spare Parts PP. Autoclavable (121 °C). Piston (PE), cylinder (PP).
Pack of 1. Pack of 3.
Cat. No. 7045 26 Cat. No. 7045 04
Adapter
PP. Pack of 1.
Outer- for bottle Cat. No.
thread thread
Membrane filter
GL 32 GL 25 7043 25 Cap
Membrane filter, 0.2 µm.
GL 32 GL 28 7043 28
PP. Cap for valve block. Pack of 10 in PE-bag,
GL 45 S* 40 7043 43
Autoclavable (121 °C). non-sterile, autoclavable
GL 32 GL 45 7043 45
Pack of 1. (121 °C). Pack of 1.
GL 45 GL 32 7043 96
GL 45 GL 38 7043 97 Cat. No. 7045 54 Cat. No. 265 35
* buttress rim
104 info@brand.de
The most frequent cause of inaccuracy in air
displacement pipettes is leakage. This arises
from damage either to the seals, pistons, or
PLT unit
tip cones. Often not detectable by the naked
eye, leaks lead to significant volume errors.
The BRAND pipette leak tester (PLT unit)
for air displacement pipettes finds even the Pipette Leak Testing Unit
smallest leaks within seconds.
PLT unit
A Closer Look...
Liquid Handling
According to monitoring of measuring instruments, air-displace- Leak rates and their detection
ment pipettes must be checked at regular intervals and the results
The leak rate is a measure of the quantity of material that flows
must be compared with the ISO 8655-2 error limits.
through a leak per time unit. For air-displacement pipettes the
However, a calibration certificate only reflects the results at the
PLT unit determines the rate through a differential pressure
time of testing. The time between these calibrations is crucial,
measurement, i.e., after creating a negative pressure, the pres-
since leaks can occur at any time.
sure rise over a given time is measured.
Well over 80% of pipettes sent in for repair have leaks and are
outside their volume tolerances, even if they don’t drip.
n Complex determinations n The pV value
While the PLT unit cannot replace regular gravimetric testing,
The leak rate is determined The pV value is the product
daily pipette checks can provide a safeguard during the periods
by considering complex of the pressure and the vol-
between calibrations. Even the smallest leaks are detected! Pro-
physical relationships. Cal- ume of a certain quantity of
cess reliability for the pipettes is thus significantly improved.
culation of the limit values a gas at the prevailing tem-
resident in the PLT must perature. This is a measure
include factors such as the of the quantity of material or
dead volume of the pipette/ the mass of the gas.
tip system, flow cross-
section of the pipette tip, n The leak rate Q L
pressure rise per time unit, The leak rate Q L is the ratio
pipette volume and type, etc. of the pV value and the
period of time during which
the gas flows through a path
cross-section.
"Start/Execute"
button
"Back" button
Select knob
LED display
106 info@brand.de
PLT unit
Liquid Handling
Testing with and without tip Dynamic or static test? Features
To check the overall pipette The dynamic test can rapidly n Limit values for the com-
system, the test is conducted determine whether a defec- mercially available single-
with a mounted, unused tip. tive piston (contamination, and multichannel pipettes
When a leak has been identi- scratches, etc.) has caused a in the volume range 1 µl to
fied, the test can be repeated leak. The pipette button must 10 ml are pre-programmed.
without a tip to determine be pushed down numerous
whether the leak arises from times during the measurement n Testing with and without
the tip cone/tip coupling period. The associated piston tips
region. movement allows a defective n Test results in seconds
piston to be recognized.
In the static test, the pipette n Patent pending
button is not pressed during
the test procedure, i.e. the
piston doesn't move. This will
only determine a leak in a gen-
eral way, without attributing it
to a particular component.
www.brand.de 107
PLT unit · Ordering Data
Ordering Data
Liquid Handling
PLT unit
(Pipette Leak Testing Unit)
Including one 1-channel pipette adapter*
each for testing of single-channel air-
displacement pipettes with tip (mounted)
and without tip, 2 plugs, 3 replacement PE
filters for the pipette adapters, universal AC
adapter, quality certificate and operating
manual. Pack of 1.
Cat. No. 7039 70
Accessories
Universal AC adapter
Input: AC 100 V - 240 V,
Filters
50/60 Hz
PE, for pipette adapter. Output: DC 6,5 V, 800 mA
Pack of 10. Pack of 1.
Cat. No. 7039 78 Cat. No. 7039 79
108 info@brand.de
BRAND calibration software is compatible
with nearly all liquid handling instruments and
glass or plastic volumetric instruments. Now
you can calibrate and track measuring instru-
EASYCAL ™ 4.0
ments to GLP and ISO 9001 standards with-
out calculators or scratch paper. EASYCAL™
4.0 software from BRAND performs all ac-
curacy and precision calculations, matches Calibration Software
them to standards and generates a report.
EASYCAL™ 4.0
A Closer Look...
Liquid Handling
n For testing of liquid handling n Continual control of the actual n Reliable transmission, calculation
instruments and volumetric results during testing by means and saving of measurement data.
instruments of glass and plastic, of a traffic-light indicator.
according to ISO 8655, ISO
n Reminder function for outstanding
4787 etc.
calibrations.
n Open software, suitable for
n Recording of primary data in
all volumetric instruments, irre-
accordance with GLP.
spective of the manufacturer.
110 info@brand.de
EASYCAL™ 4.0 · Ordering Data
Liquid Handling
Quit calibration early?
After entering three weighing values
(either manually or via data transmis-
sion from the balance), EASYCAL™
4.0 executes a background compari-
son of the results with the error lim-
its. A traffic-light indicator (green/
red) displays whether your results
exceed the error limits.
In the event of unsuccessful test-
EASYCAL™ also helps you with
ing, the test equipment can be
testing times and intervals. You
designated as a 'defective device'.
will be automatically reminded
You can cancel this selection after
which tests are due.
successful cleaning or repair.
Ordering Data
EASYCAL ™ 4.0
Items supplied:
CD-ROM with EASYCAL™ software in 5 languages (German/English/French/Spanish/
Dutch), manual and testing instructions (SOPs) in 4 languages in PDF format for single-
and multichannel pipettes, hand-held dispensers, bottletop burettes and dispensers and
volumetric measuring instruments of glass/plastic.
Version Description Cat. No.
www.brand.de 111
EASYCAL™ 4.0 · Accessories
Accessories
Liquid Handling
Micro-weighing container
The extremely small cap open-
ing and internal filter provides
simple protection against
evaporation.
112 info@brand.de
Liquid Handling
BRAND online
Liquid Handling
Notes:
www.brand.de 113
© altrendo nature/altrendo/Getty Images
Life Science
Research – and even routine applications – in the bio-
sciences are unimaginable today without high quality
plastic consumables. Increasingly sensitive detection
methods rely on ever higher quality from lab dispos-
ables.
In addition to pipette tips, filter tips, microcentrifuge
tubes and PD-Tips, BRAND Life Science also pro-
vides a variety of other high-performance products for
PCR, storage, immunology and cell culture technol-
ogy.
Life Science
Starting materials
Sensitive applications, such as enzyme tests, ammonium (DiHEMDA) and 9-octadecen-
PCR or DNA purification, require plastic dis- amide (oleamide). Both of these additives
posable products of the highest quality. The are frequently found in PP granules, and
proper selection of raw materials is a signifi- can interfere with biological tests, leading to
cant step in the manufacture of high-quality spurious results*.
products. Over many years, polypropylene The starting materials for life science prod-
and polystyrene have earned their place as ucts are carefully selected by BRAND so
preferred materials for life science consum- that substances that can dissolve out of the
ables. These materials often come into direct raw material (leachables), which could affect
contact with expensive reagents and valuable the results of biological tests, are reduced to
samples. the minimum necessary for processing. Mold
For manufacturing pipette tips, BRAND uses release agents, such as stearates and erucic
specially selected PP types, free from the acid amide, are not used in the production
additives di-(2-hydroxyethyl) methyldodecyl- process.
Advantages of PP Advantages of PS
116 info@brand.de
Life Science
Cleanroom production
Life Science
BRAND disposable items for the life sciences
are produced using the most advanced clean-
room techniques in one of the world's largest
cleanrooms for laboratory disposable items.
Continuous cleanroom monitoring, together
with precise control of environmental condi-
tions, ensures a high level of temperature
stability over the entire production area. This
uniformity, together with quality testing of the
final product by batch, guarantees the con-
sistently high quality of life science products
from BRAND.
Depending on the desired usage environment
for the final product, cleanrooms according
to ISO 14 644-1 (Classes 5, 7 and 8) are
available for manufacturing.
Surface modification
To produce optimal properties, surface mod- As exemplified by BRAND Ultra Low Reten-
ification using various physical and chemical tion tips, even PP surfaces can be modified
methods is required for the products used in to be as hydrophobic as PTFE, and such
many life sciences applications. a material will not be wetted by liquids in
Depending on the modification conditions which the surface tension has been reduced
employed, starting materials can be custom- through the use of detergents.
ized, e.g., with either hydrophilic or hydro-
phobic surfaces. To take a specific case, PS Surface Surface tension
surfaces can either be made hydrophobic so
BRAND® PP Ultra Low Retention 9 mN/m
that membrane proteins can bind, or hydro-
PTFE 19 mN/m
philic to enable cell growth.
Silicone 21.5 mN/m
Untreated PP 30 mN/m
Water 72 mN/m
www.brand.de 117
Life Science
The right quality grade should be used in each application. Consequently, BRAND
offers a range of quality grades so that the optimal one is always available.
BIO-CERT® products correspond to the Endotoxins Endotoxins refer to the components present
highest quality grade. They are sterile, free from DNA, RNases, in the outer membranes of Gram-negative bacteria. These com-
endotoxins and ATP. ponents are lipopolysaccharides that are released when cells are
destroyed. Endotoxins make up the largest group of pyrogens,
and these two are often falsely believed to be synonymous.
Depending on the concentration, these heat-stable substances
Sterility Sterility means being free of living organisms. lead to fever, circulatory collapse, shock, etc., and can be fatal
BRAND products are sterilized with beta radiation according to in very high doses. The concentration of endotoxins in products
ISO 11 137 and the AAMI Guidelines. The radiation dose is at from BRAND is determined by the kinetic-turbidimetric limulus
least 12.1 kGy. The inertGrade™ microplates are an exception; amebocyte lysate (LAL) test. The detection limit is 0.01 EU/ml.
these are sterilized with ethylene oxide due to their special surface This corresponds to an endotoxin concentration of < 1 x 10-12 g/tip
characteristics. (1 pg/tip). Freedom from endotoxins is required in pharmaceutical
An SAL (sterility assurance level) of 10-6 is guaranteed, meaning manufacture and cell culture.
no more than one part in 1 x 106 is contaminated! This level of
sterility complies with the requirements of USP 29 and the Ph.Eur.
118 info@brand.de
Microcentrifuge Tubes
Life Science
Microcentrifuge Tubes
Quality features
n High clarity.
To calculate RCF:
( )
2
n
RCF = 1.118 · r · 1000
Example:
Rotation radius r = 180 mm
(vertical distance between center
of rotation axis and bottom of
centrifuge tube)
Speed n = 6000 min-1
( 1000 )
2
6000
RCF = 1.118 · 180 ·
= 7245
www.brand.de 119
Microcentrifuge Tubes
Microcentrifuge tubes
Life Science
Pack of 1000
(1 bag).
Cat. No. 7805 07
Pack of 500
(1 bag).
Cat. No. 7805 00 BIO-CERT®
Sterile and free of endotoxins, DNA,
Pack of 3000 RNase and ATP.
(6 bags of 500). Pack of 450 (30 blister packs of 15 tubes).
Cat. No. 7805 02 Cat. No. 7804 00
Colored
Pack of 500.
Color Cat. No.
yellow 7805 21
blue 7805 22
green 7805 23
orange 7805 24
amber* 7805 25
* The amber-colored microcentrifuge tubes are particu-
larly suitable for light-sensitive reagents.
120 info@brand.de
Microcentrifuge Tubes
Life Science
0.5 ml, 1.5 ml and 2.0 ml,
with lid closure NEW!
PP. RNase-, DNA- and endotoxin-free. Lid closure to achieve especially effective sealing
and prevent accidental opening! Frosted marking area, subdivisions for approximate volume
determination.
Volume RCF max. Outer-Ø Height Pack Cat. No.
ml mm mm of
lid closure
Pack of 12 000
(6 bags of 2000).
Microcentrifuge tube racks
Cat. No. 7805 05
and adapters can be found
on page 125.
Pack of 500
(1 bag).
Cat. No. 7805 50
www.brand.de 121
Micro Tubes
Accurate
Dimensions: outer Ø
11 mm, height 47 mm.
Clear
All tubes are made of
high-transparency PP
for easy visibility of
samples.
Rugged
Withstands RCF to
17 000 g at 20 °C,
for 20 min.
Convenient
Micro tubes with ring stands
(self-standing) can be placed
in suitable racks one-handed.
Versatile
Micro tubes with screw caps
are available in different
formats, designs and quality
grades. Micro tubes of PP and
screw caps of PE or PP are
precisely matched to ensure a
secure seal.
with sealing cone with silicone seal with silicone seal,
tamper-evident screw cap
Micro tubes with sealing cone Micro tubes are sealed The tamper-evident screw
are particularly suitable for the extremely well with silicone cap guarantees the user an
storage of sensitive samples, seals, without contact be- uncontaminated sample. A vis-
since they avoid the risk of tween the sample and the ible ring acts as an anti-tamper
contamination from existing sealing ring. The containers seal, which breaks when the
silicone seals. These tubes are suitable for the storage of cap is first opened. The micro
are not autoclavable. samples in the gaseous (va- tubes have a silicone seal, and
por) phase of liquid nitrogen. are suitable for the storage of
Micro tubes with silicone seals samples in the gaseous (va-
are autoclavable at 121 °C por) phase of liquid nitrogen.
(2 bar), acc. DIN EN 285. The micro tubes with tamper-
evident screw cap are auto-
clavable at 121 °C (2 bar),
acc. DIN EN 285.
122 info@brand.de
Micro Tubes
Micro tubes
Life Science
Colored screw caps and colored
cap inserts are available sepa-
rately (page 124).
www.brand.de 123
Micro Tubes
Micro tubes
Life Science
Caps, colored
Cap Cat. No.
Color
transparent 7807 70
Tamper-evident screw caps with blue 7807 71
silicone seal, for micro tubes green 7807 72
7807 60 - 7807 64 purple 7807 73
red 7807 74
PP. Applications: -196 °C to +121 °C.
yellow 7807 75
Pack of 1000.
124 info@brand.de
Accessories
Microcentrifuge Tubes
Life Science
Microcentrifuge tube rack
PP, grey. Numbered positions for 20 microcentrifuge tubes, 1.5 ml.
Autoclavable at 121 °C (2 bar), acc. DIN EN 285.
Pack of 1.
Positions Length Width Height Cat. No.
mm mm mm
20 210 70 37 7806 05
0.5 ml adapter
for Cat. No. 7806 05
PP. Microcentrifuge tube racks can be
equipped with inserts to accommodate
0.5 ml microcentrifuge tubes. Easy perma-
nent assembly. Pack of 20.
Cat. No. 7806 08
Mini cooler
PC. Mini coolers are designed to protect a wide range of solutions (enzymes, DNA, RNA,
cell suspensions) by helping to maintain freezer temperatures on the lab bench. Durable
polycarbonate filled with non-toxic gel. Mini coolers hold twelve 0.5 ml to 2.0 ml tubes.
Bench temp. Time held Color Cat. No.
maintained
www.brand.de 125
PCR Products
PCR
Life Science
Features
Real-Time PCR?
q!PCR outstanding!
126 info@brand.de
PCR Tubes
Life Science
0.2 ml and 0.5 ml PCR tubes with attached caps are compatible with leading thermal cyclers
with heated lids. The caps are easy to open and close without tools, yet ensure a tight fit
to reduce sample evaporation. The various colors of the PCR tubes allow fast sample clas-
sification.
www.brand.de 127
PCR Strips
PCR Strips
Life Science
White PCR products offer significantly better results for qPCR, and are specifically
recommended by many thermal cycler manufacturers.
Strips of 8
with detached cap strips
PP. 8 connected 0.2 ml tubes. Separate, domed or flat caps are available in strips of 8.
They are easy to open and close without tools. The tubes have a holding strap at one end,
and the cover strips on each cap have a small lip on the side for careful, contamination-
free opening.
Pack of 125 strips, 1000 vessels or caps, total.
Capacity Color Strips of 8 Strips of 8 Strips of 8
ml PCR tubes PCR caps domed PCR caps flat*
Cat. No. Cat. No. Cat. No.
Pack of 250 strips each, 2000 tubes and 2000 caps, total.
NEW! Capacity
ml
Color Strips of 8 PCR tubes and
strips of 8 PCR caps domed
Strips of 8 PCR tubes and
strips of 8 PCR caps flat*
Cat. No. Cat. No.
NEW!
Strips of 8 PCR tubes
with attached flat individual caps
PP. 8 connected 0.2 ml or 0.15 ml tubes with attached, flat individual caps – ideal protec-
tion against contamination. The highly transparent cap makes them ideal for real-time PCR.
Available in standard and low profile versions.
Pack of 120 Strips, 960 vessels total.
Description Capacity Color Pack of Cat. No.
ml
Standard 0.2 clear 120 strips per bag 7813 32 q!PCR
Standard 0.2 white 120 strips per bag 7813 16 q!PCR NEW!
Low profile 0.15 clear 120 strips per bag 7813 33 q!PCR
Low profile 0.15 white 120 strips per bag 7813 17 q!PCR NEW!
128 info@brand.de
PCR Strips · PCR Plates
Life Science
Strips of 8 PCR tubes
with attached cap strips
PP. 8 connected 0.2 ml tubes with attached cap strips. Domed caps facilitate one-handed
operation. Pack of 125 strips, 1000 vessels, total.
Cat. No. 7813 30
Strips of 12
with detached cap strips
PP. 12 connected 0.2 ml tubes. The separate domed caps are available in strips of 12.
They ensure a secure seal. Pack of 125 strips, 1500 vessels or caps, total.
Capacity Color Strips of 12 PCR tubes Strips of 12 PCR caps
ml Cat. No. Cat. No.
24-well, non-skirted
PP. 0.2 ml wells. Suitable for most commercially available thermal cyclers.
A detailed compatibility table can be found on page 134.
Description Color Pack of Cat. No.
48-well, non-skirted
PP. 0.2 ml wells. Suitable for most commercially available thermal cyclers.
A detailed compatibility table can be found on page 134.
Description Color Pack of Cat. No.
www.brand.de 129
PCR Plates
The ultra thin-walled PCR plate design facilitates constant, rapid and precise heat transfer.
The smooth vessel interior minimizes the binding of enzymes and nucleic acid to the walls.
The rim of the wells are designed to protect against cross-contamination, allowing a reliable
seal with the sealing mats tailored to the plates.
2
NEW! Low profile Standard profile Standard profile,
elevated rim
96-well, non-skirted
PP. 0.2 ml or 0.15 ml wells. 96-well non-skirted PCR plates are suitable for most com-
mercially available thermal cyclers. A detailed compatibility table can be found on page 134.
Description Color Cut corner Pack of Cat. No.
3
1 Low profile clear H12 50 (5 plates per bag) 7813 66
Low profile white H12 50 (5 plates per bag) 7813 67
3 Standard profile, elevated rim clear H12 50 (5 plates per bag) 7813 50
Standard profile, elevated rim white H12 50 (5 plates per bag) 7813 54
Please note the thermal cycler 96-well PCR plates in standard profile can be sealed with PCR caps (strips of 8),
compatibility table on page 134. Cat. No. 7813 40 - 7813 44 and 7813 34 page 128.
130 info@brand.de
PCR Plates
Life Science
1
NEW!
raised skirt elevated rim 2
96-well, semi-skirted
PP. 0.2 ml or 0.15 ml wells. 96-well semi-skirted PCR plates can easily be labeled or
tagged with a barcode. A detailed compatibility table can be found on page 134.
Description Color Cut corner Pack of Cat. No. 3
Low profile* white H12 50 (10 plates per bag) 7813 64
50 (10 plates per bag)
Low profile* white H12 7813 65
+ 50 films for qPCR
www.brand.de 131
PCR Plates
384-well, skirted
PP. The 40 μl wells can hold sample volumes of 2 μl to 30 μl. This helps to reduce reagent
costs and shortens cycle times. The plates can be filled using multichannel pipettes or
robotic systems. A detailed compatibility table can be found on page 134.
Description Color Cut corner Pack of Cat. No.
384-well, skirted
Suitable for Roche® LightCycler® 480 and other thermal cyclers
PP. 0.03 ml tubes. The wells can hold sample volumes of 2 µl to 30 µl. This helps to reduce
reagent costs and shortens cycle times. The plates can be filled using multichannel pipettes
or robotic systems.
Description Color Cut corner Pack of Cat. No.
Skirted white A12, H12 50 (10 plates per bag) 7813 58
PCR-Box/-Rack
sorted by color (red, yellow, green, purple, blue)
PP. Suitable for sample preparation, for keeping and storing 0.2 ml single tubes,
8-strips, and 12-strips, and 96-well PCR plates. These racks can also be stacked
without lids. Withstand temperatures from -80 to +121 °C.
Pack of 5.
Cat. No. 7813 62
132 info@brand.de
PCR Sealing Mats and
Sealing Films
Life Science
PCR sealing mats have been designed to fit BRAND PCR plates exactly and reduce evapora-
tion losses by up to 75% compared to conventional systems. They are characterized by their
high flexibility, tight sealing, autoclavability and are easily pierced by pipette tips.
www.brand.de 133
Thermal Cycler Compatibility
One glance at the table will tell you which BRAND PCR plates are compatible with your thermal cycler. We are continuously updating the table
with information from manufacturers and feedback from our customers. Ask for a free sample of our PCR plates to check compatibility with your
thermal cycler (www.brand.de), with no obligation.
7814 11
7814 12
7814 15
7814 16
7813 66
7813 67
7813 68
7813 69
7813 50
7813 54
7814 00
7813 57
7813 64
7813 71
7813 72
7813 75
7813 76
7813 73
7813 74
7813 77
7813 78
7813 45
7813 47
7813 48
7813 58
rigid
White color x x x x x x x x x x x x x
Number of wells 24 48 96 96 96 96 96 96 96 96 96 384 384 384 384
Standard well x x x x x x
Low Profile well x x x x x
Cut corner H12 A12 H12 H12 H12 A12 A12 A1 H1 A24 + P24 A24 A24 A24 + P24
Agilent Technologies
AriaMx ● ●
Analytik Jena
Flex Cycler2 96 Series ● ● ● ●
Flex Cycler2 Twin 48 ● ●
Speed Cycler2 SPR ● ● ● ●
qTower 2.0 und 2.2 ● ● ● ●
APPLIED BIOSYSTEMS®
2700 ● ● ● ● ● ● ● ● ● ●
3100 ● ● ● ● ● ● ●
3130 ● ● ● ● ● ● ●
3500 ● ● ● ●
3700 ● ● ● ● ● ● ● ●
3730/ 3730x ● ● ● ● ● ● ●
9600 ● ● ● ● ● ●
9700 ● ● ● ● ● ● ● ● ● ●
9800 Fast ●
5700 ● ● ● ● ●
7000 ● ● ● ● ● ●
7300 ● ● ● ● ● ●
7500 ● ● ● ● ● ●
7700 ● ● ● ● ● ●
7900 HT ● ● ● ●
7500 Fast ●
Step One Plus ●
Veriti 0.2 ml ●
Veriti 0.1 ml ●
ViiA™ 7 ● ●
AMERSHAM® Bioscience
MegaBace® 500 ●
MegaBace® 1000 ●
MegaBace® 4000 ● ● ● ● ●
BECKMAN®
CEQ ● ●
MegaBace® 4000 ● ● ● ● ●
BIOMETRA® Analytik Jena AG
Uno ● ● ● ● ● ● ● ●
Uno II ● ● ● ● ● ● ● ● ● ● ●
T1 Thermal Cycler ● ● ● ● ● ● ● ● ● ● ● ●
Tgradient ● ● ● ● ● ● ● ●
Trobot ● ● ● ● ● ● ● ● ● ● ● ●
BIORAD®/MJ RESEARCH®
CFX 96 Touch™ ● ●
CFX 96 Connect™ ● ●
CFX Automated System II ● ● ● ●
T100 ● ●
Genecycler ●
C1000 ● ● ● ● ● ● ● ● ● ●
S100 ● ● ● ●
PTC-200 DNA-Engine ● ● ● ● ● ● ● ● ● ● ● ●
PTC-220 DNA-Eng. Dyad ● ● ● ● ● ● ● ● ● ●
PTC-221 Dyad-Disciple ● ● ● ● ● ● ● ● ●
PTC-225 DNA-Tetrad ● ● ● ● ● ● ● ● ● ● ●
PTC-240 DNA-Tetrad 2 ●
Base Station ●
iCycler ● ● ● ● ● ●
MyCycler ● ● ● ● ●
MyiQ ● ● ● ● ● ●
iQ4 ● ● ●
iQ5 ● ● ● ● ● ●
CFX96 ● ●
Chromo 4 ● ● ● ●
134 info@brand.de
Thermal Cycler Compatibility
continued
Life Science
non-skirted semi-skirted r.s.-s.* skirted
7814 11
7814 12
7814 15
7814 16
7813 66
7813 67
7813 68
7813 69
7813 50
7813 54
7814 00
7813 57
7813 64
7813 71
7813 72
7813 75
7813 76
7813 73
7813 74
7813 77
7813 78
7813 45
7813 47
7813 48
7813 58
rigid
White color x x x x x x x x x x x x x
Number of wells 24 48 96 96 96 96 96 96 96 96 96 384 384 384 384
Standard well x x x x x x
Low Profile well x x x x x
Cut corner H12 A12 H12 H12 H12 A12 A12 A1 H1 A24 + P24 A24 A24 A24 + P24
BIORAD®/MJ RESEARCH®
Opticon ● ● ●
Opticon 2 ● ● ●
Opticon 4 ● ●
Corbett Research
Palm Cycler 96 ●
Palm Cycler 384 ● ● ● ●
Eppendorf®
Mastercycler® Gradient ● ● ● ● ● ●
Mastercycler® ep ● ● ● ● ● ●
Mastercycler® ● ● ● ●
Mastercycler® M384 ● ● ● ●
Mastercycler® ep Realplex ● ● ● ● ●
Ericomp
Single Block ● ● ● ● ● ●
Twin Block ● ● ● ● ● ●
Power Block ● ● ● ●
Delta Cycler ● ● ● ● ● ● ●
Esco
Swift ● ●
Flexi
Gene ● ● ●
Genius ● ●
G-Storm
GS1/ GS4/ GSX ● ● ● ●
Labnet
MultiGene™ OptiMax ● ●
Thermo Hybaid
Multi Block System ● ● ● ● ● ● ● ● ● ● ● ● ●
Omnigene ● ● ● ● ● ● ● ● ● ● ● ● ●
Omn-E ● ● ● ● ● ● ● ● ● ●
PCR-Express ● ● ● ● ● ● ● ● ● ● ● ●
PCR-Sprint − − ● ● − − ● ● ●
pxe ● ● ● ● ● ● ● ● ●
px2 ● ● ● ● ● ● ● ● ● ● ● ●
Touchdown ● ● ● ● ● ● ● ● ● ● ● ● ●
MWG®
Primus 96 ● ● ● ● ● ● ● ● ●
Primus 384 ● ● ● ● ●
The Q LifeCycler ● ●
PEQLAB®
PeqStar 96 ● ● ● ● ● ● ●
Roche®
LightCycler® 480 ● ●
STRATAGENE®
Robocycler ● ● ● ● − ● ● ● ● ●
Robocycler Gradient ● ● ● ● ●
Mx 3000 ● ● ●
Mx 3000 P ● ● ● ●
Mx 3005 P ● ● ● ●
Mx 4000 ● ● ● ● ●
TaKaRa®
TP2400 ● ● ● ●
TP3000 ● ● ● ● ● ● ● ● ● ● ●
TECHNE®
TC-412 ● ● ● ● ● ● ● ● ● ● ● ●
Flexigene ● ● ● ● ● ● ● ● ● ● ● ● ●
Genius ● ● ● ● ● ● ●
Genius Quad ● ● ● ● ● ● ● ●
TC-512 ● ● ● ● ● ● ● ● ● ● ● ● ●
Touchgene Gradient ● ● ● ● ● ● ●
Touchgene X ● ● ● ●
TC-3000X ● ● ● − − − −
Quantica
Cyclogene ● ● ● ● ●
TRANSGENOMIC®
Wave System ● ●
● = compatible − = not compatible = no information * r. s.-s. = raised skirt semi-skirted Status as of: 1015
www.brand.de 135
Sample Storage
Life Science
Sample Storage
BRAND has developed a number of disposable
products of polypropylene and polystyrene espe-
cially for the storage of life science samples.
These products are popular for applications
including cell biology, molecular biology, drug
discovery tests, and screening.
Features
136 info@brand.de
Deep-well Plates
Life Science
Deep-well Plates
The most important application for deep-well plates is sample storage (e.g., in substance
libraries). Deep-well plates are also an important component in numerous other applications,
e.g., cultivation of microorganisms, extraction of nucleic acids and proteins, screening stud-
ies, etc.
The 1.2 ml low profile model reduces the space requirements by approx. 30%.
1
NEW!
3
96-well
U-bottom wells, non-sterile
PP and PS. Capacities 0.5 ml, 1.1 ml, 1.2 ml low profile and 2.2 ml.
Capacity Material well Height Pack Cat. No.
ml shape mm of
4
1 0,5 PP round 28.5 48 7013 46
www.brand.de 137
Deep-well Plates
Life Science
384-well
V-bottom wells, non-sterile
PP. Capacity 0.3 ml. Designed for a wide range of applications, such as High-Throughput
Screening (HTS), tests requiring a mother plate, cell and tissue cultures, serial dilutions,
reagent transfer and sample storage down to -80 °C. Deep-well plates are manufactured
in the ANSI/SLAS format and are stackable for easy storage. Pack of 48.
Cat. No. 7013 55
96-well microplate
U-bottom wells, non-sterile
PP. For volumes up to 300 µl. Compatible with virtually all leading microplate centrifuges.
Raised rings around the orifice of each well minimize possible cross-contamination.
The plates can be sealed using self-adhesive films, such as DMSO-resistant sealing film
(for more information, see page 140).
Pack of 100 (10 per bag).
Cat. No. 7013 30
138 info@brand.de
Racks · Tubes
Life Science
96 tube racks
with 0.65 ml or 1.2 ml tubes, non-sterile, for use with robots
Tubes and rack are autoclavable at 121 °C (2 bar), acc. DIN EN 285 (cover mat/strip,
TPE, not autoclavable). Tubes, racks, and lids can withstand temperatures as low as
-80 °C. The seal of the tubes is pressure-tested. Thanks to the stable connection between
the mounting plate and the racks, the stackable racks in ANSI/SLAS format are especially
well-suited for use with robots and other automated dispensing systems. Coded tubes (A1
- H12) and rack sides suitable for barcode labels simplify sample organization.
Description Material Pack Cat. No.
of
www.brand.de 139
Sealing Films
In case the microplates need not only to be covered, but also securely sealed, self-adhesive sealing
films are available. These film sheets can be easily applied on the plates and also removed without
the use of expensive equipment. They are available in different versions and are especially well-
suited for storage or cell- and tissue-culture.
q!PCR
for ELISA, PCR for ELISA, Real-Time PCR for storage, ELISA, PCR Hard rubber.
Polyester. Transparent. Polyester. High-transparency. PP. DMSO resistant. Pack of 1.
Cat. No. 7813 90 Cat. No. 7813 91 Cat. No. 7013 67 Cat. No. 7013 80
PE top, underside PP with Vinyl, acrylic adhesive. for fluorescence for luminescence
adhesive. Inert, chemically Multiply pierceable with measurement measurement
resistant. pipette tips. Vinyl, black. Vinyl, white.
Packs of 50 sheets. Packs of 100 sheets. Light-absorbent. Reflective.
Cat. No. 7013 70 Cat. No. 7013 74 Cat. No. 7013 71 Cat. No. 7013 72
Non-sterile
pack of 100 sheets
Single sheets
Cat. No. 7013 64
Cat. No. 7813 81
Sterile
pack of 50 sheets Roll
Cat. No. 7013 65 Cat. No. 7813 80
140 info@brand.de
Cryogenic Tubes
Cryogenic Tubes
Life Science
Designed for storage of biological material, such as microorganisms, human and animal cells,
Cryogenic tubes are sterile,
etc. in the gaseous phase of liquid nitrogen.
RNase-, DNase-, DNA- and
PP, graduated, outer-Ø 12.5 mm. Large frosted marking area and colored cap inserts for
endotoxin-free.
easy sample identification. Temperature stability to -196 °C, γ-sterilized (SAL 10-6) and auto-
clavable at 121 °C (2 bar), acc. DIN EN 285.
Marked with the CE symbol according to the IVD Directive 98/79 EC. Tubes without ring
stands can be centrifuged at up to an RCF of 14 000 g.
Cap inserts
PP. Fit for all sizes.
Pack of 500.
Color Cat. No.
white 1148 50
blue 1148 51
red 1148 52
green 1148 53
yellow 1148 54
www.brand.de 141
Accessories Cryogenic Tubes
Storage boxes
PC. For cryogenic tubes. Operating range (in the gaseous phase of liquid nitrogen)
-196 °C to +121 °C. Autoclavable at 121 °C (2 bar), acc. DIN EN 285.
Pack of 4.
for cryogenic tubes Positions Length Width Height Cat. No.
ml mm mm mm
Mini cooler
PC. Mini coolers are designed to protect a wide range of solutions (enzymes, DNA, RNA,
cell suspensions) by helping to maintain freezer temperatures on the lab bench. Durable
polycarbonate filled with non-toxic gel. Mini coolers hold twelve 0.5 ml to 2.0 ml tubes.
Pack of 1.
Bench temperature Time held Color Cat. No.
maintained
142 info@brand.de
BRANDplates ® Microplates
Life Science
BRANDplates ®
Microplates
Modern research methods require high-quality
disposables.
BRANDplates ® microplates can be used in all
important fields of life science. For this compre-
hensive line, three immunological and four cell
culture surfaces have been developed under the
most modern production conditions.
The product line covers a multitude of standard
applications (e.g., homogenous assays, screen-
ings) as well as applications in the fields of im-
munology and cell culture technique.
Non-treated surfaces
n
pureGrade™
pureGrade™ S
Immunology
n
immunoGrade™
hydroGrade™
lipoGrade™
Cell culture
n
cellGrade™
cellGrade™ plus
cellGrade™ premium
inertGrade™
www.brand.de 143
BRANDplates® Microplates
Life Science
BRANDplates ®
pureGrade ™
pureGrade ™ S
Microplates,
non-treated surface
pureGrade ™ S
Non-treated surface, sterile
• Sterilized via β-radiation.
• Especially suited for bacteriological assays.
144 info@brand.de
BRANDplates ®
non-treated
Life Science
Application Specifications
Application Types (see below)
Types
96-well microplates NEW! NEW!
384-well microplates
1536-well microplates
1 Standard
www.brand.de 145
BRANDplates ®
non-treated
96-well Microplates
Life Science
NEW!
black,
F-bottom 350 µl 50 (5 bags of 10 plates) 7816 15 1
UV-transparent
NEW!
4 96-well strip plates
NEW!
transparent, without grid,
F-bottom 360 µl 100 (4 bags of 25 plates) 7823 00
strips of 8 wells, not divisible
transparent, with grid,
strips of 8 wells, divisible
F-bottom 350 µl 100 (4 bags of 25 plates) 7823 01 NEW!
1
not available in USA
pureGrade ™ S
3 PS. Non-treated, sterile.
For bacteriological assays, screenings, storage, etc.
Color Bottom Well volume Pack of Cat. No.
NEW!
1 96-well standard microplates
transparent U-bottom 330 µl 50 (individually wrapped, with lid) 7816 60
transparent V-bottom 360 µl 50 (individually wrapped, with lid) 7816 61
transparent F-bottom 350 µl 50 (individually wrapped, with lid) 7816 62
transparent C-bottom 350 µl 50 (individually wrapped, with lid) 7816 63
white F-bottom 350 µl 50 (individually wrapped, with lid) 7816 65
black F-bottom 350 µl 50 (individually wrapped, with lid) 7816 68
2 96-well microplates with transparent bottom
white F-bottom 330 µl 50 (individually wrapped, with lid) 7816 70
4 black F-bottom 330 µl 50 (individually wrapped, with lid) 7816 71
146 info@brand.de
BRANDplates ®
non-treated
384-well Microplates
Life Science
pureGrade ™ (medium binding)
PS. Non-treated, non-sterile.
For homogenous assays, screenings, storage, etc.
Color Bottom Well volume Pack of Cat. No.
pureGrade ™ S
PS. Non-treated, sterile. 2
For bacteriological assays, screenings, storage, etc.
Color Bottom Well volume Pack of Cat. No.
www.brand.de 147
BRANDplates ®
non-treated
1536-well Microplates
Life Science
pureGrade ™
PS. Non-treated, non-sterile.
For homogenous assays, screenings, storage, etc.
Color Bottom Well volume Pack of Cat. No.
pureGrade ™ S
PS. Non-treated, sterile.
For bacteriological assays, screenings, storage, etc.
Color Bottom Well volume Pack of Cat. No.
148 info@brand.de
BRANDplates ® Microplates
Life Science
BRANDplates ®
immunoGrade ™
hydroGrade ™
lipoGrade ™
Microplates
for Immunoassays
Diagnosis of disease, pregnancy or verification
of doping substances – many analyses in the
modern laboratory are performed with immuno-
logical assays in microplates.
They are characterized by their high specificity
and provide the opportunity to detect the lowest
concentrations of defined substances in com-
plex liquids, such as blood serum.
www.brand.de 149
BRANDplates ® Microplates
BRANDplates ®
Life Science
immunoGrade ™ (high-binding)*
Optimized for the immobilization of IgG
• Optimized for the immobilization of IgG, offering highest binding capacity for
molecules with mixed hydrophilic and hydrophobic regions.
• The surface of choice for the majority of standard ELISAs.
• Suitable for solid phase immunoassays.
• Comparable to 'high-binding' plates from other manufacturers.
hydroGrade ™
For the immobilization of hydrophilic molecules
• Strongly hydrophilic, with high affinity to hydrophilic molecules, such as glycoproteins and
peptides, antibodies with predominantly hydrophilic regions, and nucleic acids.
• An alternative to the immunoGrade™ surface when performing solid phase assays.
• Alternative for homogeneous assays with hydrophobic molecules, that remain in solution.
lipoGrade ™
For the immobilization of hydrophobic molecules
• Strongly hydrophobic (lipophilic), for immobilization of biomolecules with predominantly
hydrophobic regions.
• An alternative to the immunoGrade™ surface for the immobilization of molecules, such
as lipoproteins or peptides.
• Specially suited for liquid phase assays when the reaction component should stay in
solution. The majority of hydrophilic biomolecules are not immobilized on this surface.
150 info@brand.de
BRANDplates ®
Immunoassays
Life Science
Application Specifications
Application Types (see below)
96-well 384-well
Types
96-well microplates NEW!
384-well microplates
www.brand.de 151
BRANDplates ®
Immunoassays
96-well Microplates
Life Science
immunoGrade ™ (high-binding)
PS, non-sterile.
Optimized for the immobilization of IgG, standard ELISA assay.
Color Bottom Well volume Pack of Cat. No.
hydroGrade ™
PS, non-sterile. Hydrophilic. For solid phase with hydrophilic molecules
and liquid phase with hydrophobic molecules, respectively.
Color Bottom Well volume Pack of Cat. No.
lipoGrade ™
PS, non-sterile. Lipophilic/hydrophobic. For solid phase with hydrophobic molecules
and liquid phase with hydrophilic molecules, respectively.
Color Bottom Well volume Pack of Cat. No.
152 info@brand.de
BRANDplates ®
Immunoassays
384-well Microplates
Life Science
immunoGrade ™ (high-binding)
PS, non-sterile.
Optimized for the immobilization of IgG, standard ELISA assay.
Color Bottom Well volume Pack of Cat. No.
hydroGrade ™
PS, non-sterile. Hydrophilic. For solid phase with hydrophilic molecules and liquid phase
with hydrophobic molecules, respectively.
Color Bottom Well volume Pack of Cat. No.
lipoGrade ™
PS, non-sterile. Lipophilic/hydrophobic. For solid phase with hydrophobic molecules
and liquid phase with hydrophilic molecules, respectively.
Color Bottom Well volume Pack of Cat. No.
www.brand.de 153
BRANDplates ® Microplates
Life Science
BRANDplates ®
cellGrade ™
cellGrade ™ plus
cellGrade ™ premium
inertGrade ™
Microplates
for Cell Culture
Cell culture is increasing in popularity in the
research and development area. Outside of
basic research, cells are cultivated today for a
number of reasons, including the production of
proteins and, in particular, as assay systems.
As cell cultures can sometimes be quite demand-
ing regarding their environment, the disposables
used for cultivation have to be of highest qual-
ity. BRAND has drawn upon its extensive expe-
rience in the production of high-quality plastic
disposables to expand the product range of
BRANDplates ® microplates into the field of cell
culture.
154 info@brand.de
BRANDplates ® Microplates
BRANDplates ®
Life Science
Surfaces for Cell Culture
Adherent cells prefer polar surfaces that possess hydrophilic functional groups.
In contrast suspension cells prefer extremly hydrophobic or hydrophilic surfaces,
that don’t allow any immobilization. Four different plate surfaces – cellGrade™,
cellGrade™ plus, cellGrade™ premium, inertGrade™ – allow the optimum combi-
nation between microplate and specific cell line.
cellGrade ™
For the cultivation of adherent cells
• Standard surface for the cultivation of adherent cell lines.
• PS-surface with different chemical groups, such as carboxyl and hydroxyl groups,
that are freely accessible.
• Surface is hydrophilic compared with non-treated PS.
• Serum components are easily bound onto the freely accessible chemical groups,
allowing an indirect adhesion of cells.
cellGrade ™ plus
For reduced-serum media cultivation of cells
• For cultivation of fastidious cell lines.
• In addition to carboxyl and hydroxyl chemical groups, free amino groups are present on
the surface.
• The surface has a protein-like composition, so cells can directly attach and spread out.
• Cells adhere faster, better rate of yield.
• Sensitive cell lines can be cultivated.
• Suited for serum-reduced cultivation of cells.
cellGrade ™ premium
Poly-D-Lysine-equivalent surface
• Poly-D-Lysine-equivalent surface, with analogous results regarding growth performance
and cell morphology.
• Optimal adhesion of cells to the surface reduces cell damage when washing frequently.
• Cultivation of cell lines with the highest demands on their environment.
• Surface suited for serum-free and serum-reduced cultivation of cells.
• Good shelf life at room temperature.
• The alternative option to biologically coated surfaces.
inertGrade ™
For cultivation of suspension cells
• Especially suited for cell cultures, when adhesion is not desired.
• Optimized surface characteristics reduce cell adhesion and protein adsorption
to a minimum.
• Inhibits early differentiation of stem cells.
• Sterilized with ethylene oxide.
www.brand.de 155
BRANDplates ®
Cell Culture
Application Specifications
Life Science
Types
96-well microplates
384-well microplates
1536-well microplates
1 Standard
156 info@brand.de
BRANDplates ®
Cell Culture
Life Science
PS, sterile. For standard cell culture applications.
Color Bottom Well volume Pack of 5 * Pack of 50 **
Cat. No. Cat. No.
1 96-well standard microplates
transparent U-bottom 330 µl – 7819 60
transparent V-bottom 360 µl – 7819 61
transparent F-bottom 350 µl – 7819 62
transparent C-bottom 350 µl – 7819 63
white F-bottom 350 µl – 7819 65
black F-bottom 350 µl – 7819 68
2 96-well microplates with transparent bottom
white F-bottom 330 µl 7819 74 7819 70
black F-bottom 330 µl 7819 75 7819 71 1
* 1 bag of 5 plates ** individually wrapped, with lid
cellGrade plus ™
PS, sterile.
For cultivation of fastidious cell lines and for serum-reduced cultivation.
Color Bottom Well volume Pack of 5 * Pack of 50 **
Cat. No. Cat. No.
1 96-well standard microplates 2
transparent F-bottom 350 µl – 7820 22
white F-bottom 350 µl – 7820 25
black F-bottom 350 µl – 7820 28
2 96-well microplates with transparent bottom
white F-bottom 330 µl 7820 34 7820 30
black F-bottom 330 µl 7820 35 7820 31
* 1 bag of 5 plates ** individually wrapped, with lid
cellGrade ™ premium
PS, sterile. For the most demanding cell lines, and for serum-reduced and serum-free
cultivation.
Color Bottom Well volume Pack of 5 * Pack of 50 **
Cat. No. Cat. No.
1 96-well standard microplates
transparent F-bottom 350 µl – 7820 82
white F-bottom 350 µl – 7820 85
black F-bottom 350 µl – 7820 88
2 96-well microplates with transparent bottomn
white F-bottom 330 µl 7820 94 7820 90
black F-bottom 330 µl 7820 95 7820 91
* 1 bag of 5 plates ** individually wrapped, with lid
inertGrade ™
PS, sterile. For cultivation of suspension and stem cells.
Color Bottom Well volume Pack of 5 * Pack of 40 *
Cat. No. Cat. No.
1 96-well standard microplates
transparent U-bottom 330 µl – 7819 00
transparent F-bottom 350 µl – 7819 02
white U-bottom 330 µl – 7819 04
white F-bottom 350 µl – 7819 05
white C-bottom 350 µl – 7819 06
black U-bottom 330 µl – 7819 07
black F-bottom 350 µl – 7819 08
black C-bottom 350 µl – 7819 09
2 96-well microplates with transparent bottom
white F-bottom 330 µl 7819 12 7819 10
black F-bottom 330 µl 7819 13 7819 11
* individually wrapped, with lid
www.brand.de 157
BRANDplates ®
Cell Culture
384-well Microplates
Life Science
cellGrade ™
PS, sterile. For standard cell culture applications.
Color Bottom Well volume Pack of 5 * Pack of 50 **
Cat. No. Cat. No.
1 384-well standard microplates
transparent F-bottom 100 µl – 7819 80
white F-bottom 100 µl – 7819 81
black F-bottom 100 µl – 7819 82
2 384-well microplates with transparent bottom
1
white F-bottom 120 µl 7819 88 7819 86
black F-bottom 120 µl 7819 89 7819 87
* 1 bag of 5 plates ** individually wrapped, with lid
cellGrade ™ plus
PS, sterile.
2 For cultivation of fastidious cell lines and for serum-reduced cultivation.
Color Bottom Well volume Pack of 5 * Pack of 50 **
Cat. No. Cat. No.
1 384-well standard microplates
transparent F-bottom 100 µl – 7820 40
2 384-well microplates with transparent bottom
white F-bottom 120 µl 7820 48 7820 46
black F-bottom 120 µl 7820 49 7820 47
* 1 bag of 5 plates ** individually wrapped, with lid
cellGrade ™ premium
PS, sterile. For the most demanding cell lines, and for serum-reduced and serum-free
cultivation.
Color Bottom Well volume Pack of 5 * Pack of 50 **
Cat. No. Cat. No.
1 384-well standard microplates
transparent F-bottom 100 µl – 7821 00
2 384-well microplates with transparent bottom
Lids for BRANDplates ®
white F-bottom 120 µl 7821 08 7821 06
microplates can be found
on page 164. black F-bottom 120 µl 7821 09 7821 07
* 1 bag of 5 plates ** individually wrapped, with lid
1536-well Microplates
cellGrade ™
PS, sterile. For standard cell culture applications.
Color Bottom Well volume Pack of Cat. No.
158 info@brand.de
BRANDplates ®
Insert System
Life Science
NEW!
BRANDplates ®
Insert System
The new BRANDplates® Insert System offers
an innovative expansion of the existing cell cul-
ture product line at BRAND. Perfect for manual
applications and for use with pipetting robots!
www.brand.de 159
BRANDplates ®
Insert System
NEW! For manual and automated cell and tissue culture work.
The BRAND Insert System, co-developed with the Fraunhofer Society, includes
two different cell culture plates and two types of cell culture inserts, which can
also be used in combination.
160 info@brand.de
BRANDplates ®
Insert System
A Closer Look...
Life Science
The well and insert are perfectly n Innovative, specially designed
coordinated: wells with side feeding ports
The guide grooves in the support collars for n Track-etched PC and PET mem-
the wells in the standard 24-well plate hold branes
the guide ridges of the insert in position.
This prevents the individual inserts from ro- n Sterile according to ISO 11 137
tating – the feed ports on the wells remain and AAMI Guidelines, SAL 10-6
open. At the same time, the guide ridges Guide grooves in the Guide Feeding n Non-cytotoxic according to
center the insert in the well. standard 24-well plates ridge port
ISO 10 993-5, endotoxins-free
(< 0.01 EU/ml), free from DNA,
Hanging Inserts DNase, and RNase.
The distance from the well bottom to the
insert membrane is 4 mm.
0.4 µm Co-culture, transport studies, secretion studies, cell polarity studies, etc.
1.0 µm Co-culture, transport studies, secretion studies, etc.
3.0 µm Migration studies, chemotaxis studies, metastasis experiments, etc.
8.0 µm Migration studies, chemotaxis studies, metastasis experiments, etc.
See also the construction of full-thickness skin models by the Fraunhofer IGB
(www.tissue-factory.com)
www.brand.de 161
BRANDplates ®
Insert System
Ordering Data
Life Science
* patent pending
* patent pending
162 info@brand.de
BRANDplates ®
Overview
At a Glance
Life Science
Use our BRANDplates® microplates
Selection Guide at www.brand.de
96-well Microplates
Type Non-treated Immunological surfaces Cell culture surfaces
Standard
Color Bottom / pureGrade™ pureGrade™ S immunoGrade™ hydroGrade™ lipoGrade™ cellGrade™ cellGrade™ cellGrade™ inertGrade™
Well volume plus premium
transparent U / 330 µl 7816 00 7816 60 7817 20 7817 80 7818 40 7819 60 – – 7819 00
transparent V / 360 µl 7816 01 7816 61 7817 21 – 7818 41 7819 61 – – –
transparent F / 350 µl 7816 02 7816 62 7817 22 7817 82 7818 42 7819 62 7820 22 7820 82 7819 02
transparent C / 350 µl 7816 03 7816 63 7817 23 7817 83 7818 43 7819 63 – – –
white U / 330 µl 7816 04 – 7817 24 7817 84 7818 44 – – – 7819 04
white F / 350 µl 7816 05 7816 65 – – – 7819 65 7820 25 7820 85 7819 05
white C / 350 µl – – 7817 26 7817 86 7818 46 – – – 7819 06
black U / 330 µl 7816 07 – 7817 27 7817 87 7818 47 – – – 7819 07
black F / 350 µl 7816 08 7816 68 – – – 7819 68 7820 28 7820 88 7819 08
black C / 350 µl – – 7817 29 7817 89 7818 49 – – – 7819 09
with transparent bottom
Color Bottom / pureGrade™ pureGrade™ S immunoGrade™ hydroGrade™ lipoGrade™ cellGrade™ cellGrade™ cellGrade™ inertGrade™
Well volume plus premium
white F / 330 µl 7816 10 7816 70 – – 7818 50 7819 70 7820 30 7820 90 7819 10
white* F / 330 µl – – – – 7818 52 7819 74 7820 34 7820 94 7819 12
black F / 330 µl 7816 11 7816 71 7817 31 – 7818 51 7819 71 7820 31 7820 91 7819 11
black* F / 330 µl – – 7817 32 – 7818 53 7819 75 7820 35 7820 95 7819 13
with UV film bottom
Color Bottom / pureGrade™ pureGrade™ S immunoGrade™ hydroGrade™ lipoGrade™ cellGrade™ cellGrade™ cellGrade™ inertGrade™
Well volume plus premium
transparent F / 350 µl 7816 14 1 – – – – – – – –
black F / 350 µl 7816 15 – – – – – – – –
Strip plates
Color Bottom / pureGrade™ pureGrade™ S immunoGrade™ hydroGrade™ lipoGrade™ cellGrade™ cellGrade™ cellGrade™ inertGrade™
Well volume plus premium
transparent,
F / 360 µl 7823 00 – 7823 05 – – – – – –
without grid
transparent,
F / 350 µl 7823 01 – 7823 06 – – – – – –
with grid
1
not available in USA * Pack of 5
www.brand.de 163
Lids for BRANDplates ®
Microplates
Accessories
Life Science
164 info@brand.de
Cuvettes
Life Science
Cuvettes
For more than 25 years, BRAND has been one
of the leading manufacturers of disposable plas-
tic cuvettes. Macro and semi-micro cuvettes
of PS and PMMA are now standard in every
laboratory. This product line was extended with
the plastic UV-Cuvettes. The UV-transparent cu-
vettes are available in various types and replace
sensitive and expensive glass or quartz cuvettes
in many areas.
Quality features:
www.brand.de 165
UV-Cuvette
Life Science
UV-Cuvette micro
Center height: 8.5 mm or 15 mm
Specially designed for photometric determination of proteins, ssDNA, dsDNA,
RNA and oligonucleotides in the UV range. Usable starting from 230 nm.
Ideally suited for measurements at 260 nm, 280 nm and in the visible range.
Standard 10 mm light path. Sample volumes as small as 70 µl are sufficient.
Individually wrapped UV-Cuvettes micro are free of DNase, DNA and RNase!
Center height Pack of Cat. No.
mm
blue 7592 40
yellow 7592 41
green 7592 42
orange 7592 43
166 info@brand.de
Cuvettes
Life Science
Standard Cuvettes macro and semi-micro
PS and PMMA. Grouped by mold cavity number. 10 mm light path.
Pack of 1000 (10 boxes of 100 cuvettes per box.)
Description Material Cat. No.
Cuvette rack
PP, grey. Numbered positions. Autoclavable (121 °C).
Suitable for standard 10 mm path-length cuvettes. Pack of 1.
Description Length Width Height Cat. No.
mm mm mm
PS 3 120 mm 7598 00
www.brand.de 167
Cuvettes
Technical Data
Life Science
Overview Table
Cuvette type Filling volume Dimensions Range of application Standard deviation
min. max. window (w x h) in extinction units
40 Dioxane – – +
DMF – – +
30
Ethyl acetate – – +
20 Hexane – + –
10 Hydrochloric acid, 36% + – +
Wavelength (nm) Hydrofluoric acid, 10% + + +
0
Isopropanol + + +
200 220 240 260 280 300 320 340 360 380 400
Nitric acid, 65% – – +
UV-Cuvette micro Standard cuvette PMMA Sodium hydroxide + + +
UV-Cuvette semi-micro/macro Standard cuvette PS * Short time resistance, 30 min. Longer-term storage of these chemicals
should be confirmed by the user. Request a free sample.
To achieve reproducible results: Before the actual measurement, always determine
the blank value for the cuvette, and determine the linear range of measurement by
means of a calibration curve.
168 info@brand.de
Life Science
BRAND online
Life Science
Notes:
www.brand.de 169
© allison mcd photography/Flickr open/Getty Images
Volumetric
Instruments
Precision analysis requires measuring instruments
with consistently high precision. Every BLAUBRAND®
volumetric instrument is individually calibrated. Statisti-
cal process control ensures with a certainty of at least
99.6 % (AQL ≤ 0.4) compliance with predefined speci-
fications. BLAUBRAND® – quality from the very start.
Volumetric Instruments
Volumetric Instruments
Volumetric Instruments
Precision
Class A/AS
The intended application determines
which measuring instrument should Volumetric instruments of class A/AS are within the error limits defined by DIN and ISO and
be used. Precision analysis requires carry the DE-M marking.
measuring instruments with consis-
tently high precision. Even the most Marks and inscriptions in high contrast blue
sophisticated automated systems enamel. It represents the optimum combina-
will only deliver reliable results if tion of resistance and readability.
the volumetric instruments used
in preparing the samples meet the
required precision. Marks and inscriptions in ETERNA amber
BLAUBRAND® volumetric instru- stain. It diffuses into the glass surface
ments represent the highest degree and can only be removed by abrasion. It is
of technical perfection. They are used for volumetric instruments which are
made of glasses which are highly subjected to particularly aggressive cleaning
resistant to nearly all chemical sub- Graduation: methods.
stances – a prerequisite for durable ring marks at major graduations
precision.
Calibration
Class B
Every glass volumetric measuring
instrument is individually calibrated.
Computer-controlled systems ensure Volumetric instruments of class B are within twice the error limits for class A/AS as defined
maximum precision in a fully auto- by DIN and ISO.
mated production line.
Marks and inscriptions in high contrast
'Statistical Process Control' (SPC)
white enamel.
guarantees production of volumetric
instruments with the smallest devia-
tion from nominal capacity (accu- ETERNA marks and inscriptions in amber
racy) and narrow scatter of individual stain are particularly resistant to aggressive
values (coefficient of variation). cleaning methods.
Types of calibration
'TC, In': The contained quantity of
Graduation:
liquid corresponds exactly to the
short graduation marks
capacity indicated on the instru-
ment (e.g., graduated cylinders and
volumetric flasks).
Firing
'TD, Ex': The delivered quantity of
liquid corresponds exactly to the High quality inks combined with carefully controlled annealing processes provide durable
capacity indicated on the instrument graduations. The most modern manufacturing technology is used for controlled heating and
(e.g., pipettes and burettes). cooling. The maximum temperature is between 400 and 550 °C, depending on the type of
glass.
Silk-screening
Silk-screen printing follows calibra-
Quality assurance, AQL ≤ 0,4
tion. BRAND uses flexible screen
stencils for all graduated pipettes, The QM system is DIN EN ISO 9001 certified, and includes ongoing testing during the
burettes, graduated cylinders, and manufacturing process with final random sample testing in compliance with DIN ISO 3951
mixing cylinders. Thus, the volume at final inspection. The accepted quality level (AQL) is less than or equal to 0.4 (see page
markings on the stencil exactly 318 for further information.)
match the calibration marks, at all
capacities. Even intermediate vol-
Heating of volumetric instruments
umes on BRAND volumetric instru-
ments are extremely precise. For All reusable BLAUBRAND® and SILBERBRAND volumetric instruments can be heated up
silk-screen printing BRAND uses to 250 ºC in a dry box or sterilizer without having to worry about a change in volume. Use
special inks designed for volumetric only gradual heating and cooling because abrupt temperature changes cause thermal stress
instruments. and may result in glass breakage.
172 info@brand.de
Certificates
BLAUBRAND ® Volumetric
Volumetric Instruments
instruments with certificate
* as of January 1, 2015
BRAND will change the mark on volumetric instruments
to "DE-M" starting January 1, 2015.
Certificates
Batch certificate Individual certificate USP certificate DAkkS calibration certificate
The batch number, and mean The batch number, the Selected BLAUBRAND volu- ®
This certificate is issued by the
value plus standard deviation individual serial number, the metric instruments can be deliv- DAkkS calibration laboratory at
for the batch, along with the measured volume, measure- ered with volume error limits in BRAND. Due to the extensive
date of issue are documented ment uncertainty, and the date compliance with United States international cooperation of the
on the certificate. The measur- of issue are documented on Pharmacopeia (USP) with batch DAkkS (formerly DKD) Ger-
ing instrument bears the batch the certificate. The measuring certificate and on request, with man accreditation body (EA
number that is burnt in using instrument bears the batch individual certificate. Agreement, ILAC-MRA), the
easy-to-read digital numbers: and serial numbers that are The measuring instrument DAkkS calibration certificate
burnt in using easy-to-read shows the requested error limit is internationally recognized.
15.02 digital numbers: and the USP sign. Both the instrument and the
(Batch number: certificate show an individual
serial number and the labora-
year of manufacture/batch) 15.02 0756
tory's registration number as
(Individual serial number: year
well as the year and month of
of manufacture/batch/con-
issue.
secutive instrument number)
Ordering note
If you require all of your
BLAUBRAND® volumetric How it's done! Ordering example:
instruments to come from one
Volumetric instrument/ BRAND Cat. No.
batch for statistical testing
Certificate
equipment monitoring, please
so indicate by placing a 2 in 100 ml Volumetric flask with batch certificate 372 49
front of the BRAND catalog
number. With dealer-specific 100 ml Volumetric flask, total order from one batch (to the extent possible) 2 372 49
catalog numbers please add the 100 ml Volumetric flask with individual certificate 9 372 49
phrase "from one batch". Re-
quests for individual, USP and 100 ml Volumetric flask with USP individual certificate USP 369 49
DAkkS certificates should be
100 ml Volumetric flask with DAkkS calibration certificate DAkkS 372 49
handled in the same manner.
www.brand.de 173
Bulb Pipettes
Single Volume
Volumetric Instruments
Manufacturer
174 info@brand.de
Bulb Pipettes
Volumetric Instruments
Capacity Error limit Cat. No.
ml ± ml
BLAUBRAND®, class AS,
0.5 0.005 297 01
DE-M marking
1 0.008 297 02
AR-GLAS®. DIN EN ISO 648. 2 0.010 297 03
Calibrated to deliver (TD, Ex). 2.5 0.010 297 04
Incl. one batch certificate. 3 0.010 297 05
0.5 to 2 ml: pack of 12; 4 0.015 297 06
2.5 to 100 ml: pack of 6. 5 0.015 297 07
6 0.015 297 08
7 0.015 297 09
8 0.02 297 10
9 0.02 297 11
10 0.02 297 12
15 0.03 297 13
20 0.03 297 14
25 0.03 297 15
30 0.03 297 16
40 0.05 297 17
NEW!
50 0.05 297 18
100 0.08 297 19
www.brand.de 175
Bulb Pipettes
Capacity
ml
Error limit
± ml
Cat. No.
Bulb pipettes, 1 mark
SILBERBRAND ETERNA, class B
0.5 0.007 295 01
1 0.010 295 02 AR-GLAS®. DIN EN ISO 648.
2 0.015 295 03 Calibrated to deliver (TD, Ex).
3 0.015 295 05 0.5 to 2 ml: pack of 12;
5 0.025 295 07 3 to 100 ml: pack of 6.
10 0.03 295 12
15 0.04 295 13
20 0.04 295 14
25 0.04 295 15
50 0.07 295 18
100 0.12 295 19
176 info@brand.de
Graduated Pipettes
Graduated Pipettes
Volumetric Instruments
In the DIN EN ISO 835 standard, the
waiting time for graduated pipettes
BLAUBRAND ® Graduated Pipettes, Class AS
was reduced from 15 to 5 seconds
and the type 2, total delivery, nominal
volume at top, was included. Type 2 Type 1 Type 3
We recommend the graduated
pipette type 2 as with these pipettes total partial total
the meniscus needs to be set only delivery delivery delivery
once to achieve exact pipetting. nominal volume zero point zero point
In contrast, with types 1 and 3, there at the top at the top at the top
is a risk of over-dispensing while set-
ting the meniscus for partial volumes
the second time. In such a case, the
sample would have to be prepared
again.
www.brand.de 177
Graduated Pipettes
!
Graduated pipettes, Type 2, total delivery, USP N E W
BLAUBRAND®, class AS, nominal volume at the top, DE-M marking
AR-GLAS®. DIN EN ISO 835. Calibrated to deliver (TD, Ex). Incl. USP batch certificate.
Pack of 12.
Capacity Subdivision Error limit Cotton plug Cat. No.
ml ml ± ml upper end
178 info@brand.de
Graduated Pipettes
Volumetric Instruments
Graduated pipettes, Type 2, total delivery NEW!
SILBERBRAND ETERNA, class B, nominal volume at the top
AR-GLAS®. DIN EN ISO 835. Calibrated to deliver (TD, Ex).
Pack of 12 (20, 25 ml: pack of 6).
Capacity Subdivision Error limit Cotton plug Cat. No.
ml ml ± ml upper end
www.brand.de 179
Graduated Pipettes
180 info@brand.de
Graduated Pipettes
Volumetric Instruments
Graduated pipettes, Type 3, total delivery
SILBERBRAND ETERNA, class B, zero point at the top
AR-GLAS®. DIN EN ISO 835. Calibrated to deliver (TD, Ex).
0.5 to 10 ml: Pack of 12; 20 and 25 ml: Pack of 6.
Capacity Subdivision Error limit Cotton plug Cat. No.
ml ml ± ml upper end
www.brand.de 181
Graduated Pipettes
Volumetric Instruments
182 info@brand.de
Volumetric Flasks
Volumetric Flasks BRAND volumetric flasks provide the highest All BLAUBRAND® volumetric flasks
Volumetric Instruments
accuracy. Strict statistical quality controls are supplied with a batch number and
ensure maintenance of these high standards. an accompanying batch certificate in
Volumetric flasks are indispensable for pre- the original packaging.
paring dilutions and standard solutions. On request, they are also available
Standard volumetric flasks are supplied with with an individual certificate, USP in-
a square-grip PP stopper with a dripping tip. dividual certificate or DAkkS calibra-
These stoppers reduce the danger of break- tion certificate (ordering information,
age if a flask tips over, and help to prevent it page 173).
from rolling off the workbench.
www.brand.de 183
Volumetric Flasks
Volumetric Instruments
Volumetric flasks
BLAUBRAND®, class A, DE-M marking
Boro 3.3. DIN EN ISO 1042. Calibrated to contain (TC, In). Incl. one batch certificate.
5 to 500 ml: Pack of 2; 1000 to 10000 ml: Pack of 1.
Capacity Error limit Neck size with PP stopper with glass stopper
ml ± ml Cat. No. Cat. No.
184 info@brand.de
Volumetric Flasks
Volumetric Instruments
Volumetric flasks, USP
BLAUBRAND®, class A, DE-M marking
Boro 3.3. DIN EN ISO 1042. Calibrated to contain (TC, In). Incl. one USP batch
certificate. Pack of 2; 1000 ml: pack of 1.
Capacity Error limit Neck inner Ø Neck size with PP stopper with glass stopper
ml ± ml mm Cat. No. Cat. No.
Volumetric flasks
BLAUBRAND® ETERNA, class A, DE-M marking
Boro 3.3. DIN EN ISO 1042. Calibrated to contain (TC, In). Incl. one batch certificate.
5 to 500 ml: Pack of 2; 1000 and 2000 ml: Pack of 1.
Capacity Error limit Neck size with PP stopper
ml ± ml Cat. No.
www.brand.de 185
Volumetric Flasks
Volumetric Instruments
186 info@brand.de
Volumetric Flasks
Volumetric Instruments
Volumetric flasks, amber glass
BLAUBRAND®, class A, DE-M marking
Boro 3.3. DIN EN ISO 1042. Calibrated to contain (TC, In).
Interchangeable PP or amber glass stopper. Incl. one batch certificate.
5 to 500 ml: Pack of 2; 1000 ml: Pack of 1.
Capacity Error limit Neck size with PP stopper with glass stopper
ml ± ml Cat. No. Cat. No.
Boro 3.3. DIN EN ISO 1042. Calibrated to contain (TC, In). Incl. USP batch certificate.
Pack of 2; 1000 ml: pack of 1.
Capacity Error limit Neck inner Ø Neck size with PP stopper with glass stopper
ml ± ml mm Cat. No. Cat. No.
10 W 0.04 370 45
20 0.04 370 46
25 0.04 370 47
50 0.06 370 48
100 0.10 370 49
200 0.15 370 50
250 0.15 370 51
500 0.25 370 52
1000 0.4 370 53
W = Wide neck
www.brand.de 187
Volumetric Flasks
Volumetric Instruments
Volumetric flasks
SILBERBRAND, class B
Boro 3.3. DIN EN ISO 1042. Calibrated to contain (TC, In).
10 to 500 ml: pack of 2; 1000 to 5000 ml: pack of 1.
Capacity Error limit Neck size with PP stopper
ml ± ml Cat. No.
188 info@brand.de
Volumetric Flasks
made of Plastic
Volumetric Instruments
Error limits according to class A, DIN EN ISO 1042. Calibrated to contain (TC, In).
Incl. one batch certificate. Autoclavable, easy to clean. PFA screw cap with molded seal-
ing ring. Exposure to temperatures up to 121 ºC (autoclaving) will not cause permanently
exceeded error limits! Cleaning up to max. 60 °C is recommended to preserve marks and
inscriptions. Pack of 1.
Capacity Error limit Height* Thread Cat. No.
ml ± ml mm
10 W 0.04 90 GL 18 362 08
25 0.04 108 GL 18 362 20
50 0.06 143 GL 18 362 28
100 0.10 166 GL 18 362 38
250 0.15 222 GL 25 362 48
500 0.25 262 GL 25 362 54
W = Wide neck * without screw cap
www.brand.de 189
Graduated Cylinders
Volumetric Instruments
Graduated Cylinders
BRAND graduated and mixing cylinders pro- All BLAUBRAND® graduated cylinders are
vide the highest accuracy. Strict statistical supplied with a batch number and an accom-
quality controls ensure maintenance of these panying batch certificate in the original pack-
high standards. aging. On request, they are also available
with an individual certificate, USP individual
certificate or DAkkS calibration certificate
(ordering information, page 173).
190 info@brand.de
Graduated Cylinders
Volumetric Instruments
Graduated cylinders, tall form
BLAUBRAND® or BLAUBRAND® ETERNA, class A, DE-M marking,
ring marks at major graduations
Boro 3.3. DIN EN ISO 4788. Calibrated to contain (TC, In). Incl. one batch certificate.
With spout and hexagonal base. 5 to 500 ml: pack of 2; 1000 and 2000 ml: pack of 1.
Capacity Subdivision Error limit Height BLAUBRAND® BLAUBRAND® ETERNA
ml ml ± ml mm Cat. No. Cat. No.
!
Graduated cylinders, tall form, USP N E W
BLAUBRAND®, class A, DE-M marking, ring marks at major graduations
Boro 3.3. DIN EN ISO 4788. Calibrated to contain (TC, In). Incl. USP batch certificate.
With spout and hexagonal base. 5 to 500 ml: pack of 2; 1000 ml: pack of 1.
Capacity Subdivision Error limit Height BLAUBRAND®
ml ml ± ml mm Cat. No.
5 0.1 0
.05 USP 115 328 05
10 0.2 .10 USP
0 140 328 08
25 0.5 0
.17 USP 170 328 20
50 1 .25 USP
0 200 328 28
100 1 0.5 USP 260 328 38
250 2 1
.0 USP 335 328 48
500 5 .0 USP
2 365 328 54
1000 10 3
.0 USP 465 328 62
2000 20 6
.0 USP 505 328 64
www.brand.de 191
Graduated Cylinders
Volumetric Instruments
10 1 0.3 90 420 08
25 1 0.5 115 420 20
50 2 1 145 420 28
100 2 1 165 420 38
250 5 2 195 420 48
500 10 5 250 420 54
1000 20 10 285 420 62
2000 50 20 340 420 64
192 info@brand.de
Graduated Cylinders
Dispensers, tilt measure
Volumetric Instruments
Mixing cylinders
BLAUBRAND®, class A, DE-M marking, or SILBERBRAND ETERNA, class B
Boro 3.3. DIN EN ISO 4788. Calibrated to contain (TC, In). BLAUBRAND® mixing
cylinders incl. one batch certificate. With PP stopper and hexagonal base.
10 to 500 ml: Pack of 2; 1000 ml: Pack of 1.
Capacity Subdivision Neck size Height BLAUBRAND® SILBERBRAND ETERNA
ml ml mm Cat. No. Cat. No.
www.brand.de 193
Plastic Graduated Cylinders
Volumetric Instruments
194 info@brand.de
Plastic Graduated Cylinders
Volumetric Instruments
Graduated cylinders, PP
tall form, class B, with blue printed scale or embossed scale
PP, high clarity. DIN 12 681 and ISO 6706. Calibrated to contain (TC, In). Exposure to
temperatures up to 80 °C will not cause permanently exceeded error limits! Cleaning tem-
perature below 60 °C is recommended to preserve marks and inscriptions.
Capacity Subdivision Error limit Height Pack blue scale embossed scale
ml ml ± ml mm of Cat. No. Cat. No.
www.brand.de 195
Burettes
and Automatic Burettes
Burettes
Volumetric Instruments
Specifications
Burettes and automatic burettes are always calibrated to deliver (TD, Ex).
Waiting times:
BLAUBRAND® – waiting time 30 sec. SILBERBRAND – no waiting time
Note: A waiting time is usually not neccessary if a titration is performed
(see DIN EN ISO 385).
All BLAUBRAND® burettes and automatic burettes are supplied with a batch
number and an accompanying batch certificate in the original packaging. On
request, they are also available with an individual certificate, USP individual
certificate or DAkkS calibration certificate (ordering information, page 173).
196 info@brand.de
Burettes
Volumetric Instruments
Burettes, lateral stopcock
BLAUBRAND®, class AS, DE-M marking
Boro 3.3. DIN EN ISO 385. Calibrated to deliver (TD, Ex). Schellbach stripe.
Incl. one batch certificate. Length approx. 800 mm. Pack of 2.
Capacity Subdivision Error limit Stopcock with Cat. No.
ml ml ± ml
www.brand.de 197
Burettes
Volumetric Instruments
198 info@brand.de
Burettes
Volumetric Instruments
Burettes, lateral stopcock
SILBERBRAND, class B
Boro 3.3. DIN EN ISO 385. Calibrated to deliver (TD, Ex). Schellbach stripe.
Length approx. 800 mm. Pack of 2.
Capacity Subdivision Error limit Stopcock with Cat. No.
ml ml ± ml
www.brand.de 199
Burettes
Volumetric Instruments
200 info@brand.de
Burettes
Volumetric Instruments
The Compact Burette from BRAND.
Compact Burettes
BLAUBRAND®, class AS, DE-M marking
Boro 3.3, removable PTFE stopcock. DIN EN ISO 385. Calibrated to deliver (TD, Ex).
Items supplied: Burette tube with Schellbach stripe; Stopcock with precision tip.
Incl. one batch certificate. Pack of 1.
Capacity Subdivision Error limit Length Cat. No.
ml ml ± ml mm
Compact Burettes
SILBERBRAND
AR-GLAS®, removable PTFE stopcock. Error limits according to class B, DIN EN ISO 385.
Calibrated to deliver (TD, Ex). Items supplied: Burette tube with Schellbach stripe;
Stopcock with precision tip. Pack of 1.
Capacity Subdivision Error limit Length Cat. No.
ml ml ± ml mm
www.brand.de 201
Burettes
Volumetric Instruments
SILBERBRAND, AR-GLAS ®
10* 350 139 33
SILBERBRAND, AR-GLAS® 25* 410 139 36
SILBERBRAND, AR-GLAS® 50* 610 139 38
202 info@brand.de
Burettes
Volumetric Instruments
Compact burettes, 'Economy'
SILBERBRAND
AR-GLAS®, removable PTFE stopcock with PP tip. Error limits according to class B, DIN
EN ISO 385. Calibrated to deliver (TD, Ex). Items supplied: burette tube, stopcock with PP
tip.
Pack of 2.
Capacity Subdivision Error limit Length Cat. No.
ml ml ± ml mm
Burette tubes
SILBERBRAND
AR-GLAS®. Error limits according to class B, DIN EN ISO 385.
Calibrated to deliver (TD, Ex). Discharge tube with outer Ø 8 mm.
For use with PTFE stopcock (Cat. No. 118 00).
Pack of 2.
Capacity Subdivision Error limit Length Cat. No.
ml ml ± ml mm
PTFE stopcock
for burette tubes
Spare burette tip
for PTFE stopcock
With PP tip. Complete with mounting tool.
Pack of 1. PP, with screw cap. Pack of 1.
Cat. No. 118 00 Cat. No. 116 00
www.brand.de 203
Automatic Burettes
SILBERBRAND
Burette: AR-GLAS®. Error limits according to class B, DIN EN ISO 385.
Calibrated to deliver (TD, Ex). Schellbach stripe. Automatic zeroing.
Pack of 1.
Capacity Subdivision Error limit With bottle Total height Cat. No.
ml ml ± ml ml mm
204 info@brand.de
Automatic Burettes
Volumetric Instruments
Automatic burettes, Pellet pattern,
with intermediate stopcock
BLAUBRAND®, class AS, DE-M marking
Boro 3.3. DIN EN ISO 385. Calibrated to deliver (TD, Ex). Schellbach stripe.
Incl. one batch certificate. Available with needle valve stopcock with PTFE spindle (PTFE
key in intermediate stopcock) or STJ glass stopcock (glass key in intermediate stopcock).
Automatic zeroing. Intermediate stopcock 4 NS/19 for recirculating residual liquid.
Total height approx. 1 m incl. 2000 ml bottle (soda-lime glass). Pack of 1.
www.brand.de 205
Automatic Burettes
Volumetric Instruments
206 info@brand.de
Automatic Burettes
Volumetric Instruments
Automatic burettes, Pellet pattern,
without intermediate stopcock
SILBERBRAND, amber glass
Borosilicate glass 5.4.
25 ml: Error limits according to class B, DIN EN ISO 385.
50 ml: DIN EN ISO 385, class B.
Calibrated to deliver (TD, Ex). Available with titration stopcock with PTFE key or glass key.
Total height 25 ml approx. 0.7 m, total height 50 ml approx. 1 m incl. 2000 ml bottle
(soda-lime glass). Pack of 1.
www.brand.de 207
Automatic Burettes
208 info@brand.de
Automatic Burettes
Volumetric Instruments
Please order supports, burette
clamps and rubber bellows sepa-
rately (pages 212-213).
www.brand.de 209
Automatic Burettes
Volumetric Instruments
210 info@brand.de
Spare Burette Stopcocks
Volumetric Instruments
Spare burette tubes
for Compact automatic burettes with automatic zeroing
BLAUBRAND®, incl. batch certificate. Pack of 1.
Description Capacity Length Cat. No.
ml mm
Bases
Spare filling tube Pack of 1.
PVC, transparent. For bottle Ø bottle Cat. No.
Inner-Ø 5 mm, Outer-Ø 7 mm, length 1 m. ml mm
Pack of 1.
500 75 237 25
Cat. No. 115 25 1000 94 237 28
Reservoir bottles
PE-LD. Narrow-mouth bottle with tube bushing. Pack of 1.
Capacity Ø Height Thread Cat. No.
ml mm mm GL
www.brand.de 211
Burette Accessories
Volumetric Instruments
Pumphead
for glass reservoir bottle,
compact automatic burette
Boro 3.3. NS 29/32. Fitting length
185 mm. Nozzle outer diameter 7 mm.
Pack of 1.
Description Cat. No.
Rubber bellows
for automatic burettes
Orange colored, single-bulb rubber
bellows. With valve and tubing. Pack of 1.
Cat. No. 234 00
Burette clamps
Zinc alloy, nickel-plated.
PVC-coated clamps.
Pack of 1.
Description Cat. No.
Joint Clip
Stainless-steel Neck size 29/32.
Pack of 1.
for 1 burette 165 15
for 2 burettes 165 20 Cat. No. 556 18
212 info@brand.de
Burette Accessories
Volumetric Instruments
Burette clamp
Alluminium die cast. Two burettes up to Very sturdy design, easy to operate. Integral
50 ml can be mounted in seconds. With support connector. Ideal for use with burette
plastic-coated roller grips. Comfortable support (Cat. No. 238 82). Pack of 1.
height adjustment. Graduations remain
Cat. No. 5780 00
visible.
Burette clamps
PP, white. To be mounted on support rods
of 8 to 14 mm Ø. Easily affixes the burette
by clamping with a non-corroding spring.
Pack of 1.
Description Cat. No.
Burette support
Baseplate: PP with rubber feet minimize
sliding and protect bench-top.
Support rod: Stainless steel.
Includes baseplate 210 x 155 mm (L x W).
Support rod 550 x 12 mm (L x diameter)
Pack of 1.
Cat. No. 238 82
Burette covers
PP. Ribbed inside to prevent suction
attachment during titration. Pack of 10.
Inner Ø Height Cat. No.
mm mm
20 30 164 00
www.brand.de 213
Spare Burette Stopcocks
Needle-valve stopcocks
BISTABIL, Boro 3.3/PTFE
Valve housing with precision Boro 3.3 burette tip.
Interchangeable PTFE valve spindle, PP cap and retention device.
Valve opening 0-2.5 mm. Pack of 1.
For burette capacity Lateral needle-valve stopcock Straight needle-valve stopcock
ml Cat. No. Cat. No.
2 - 10 821 20 822 20
25 821 21 822 21
50 821 22 822 22
2 - 50 821 70 821 70
Glass stopcocks
BISTABIL, Boro 3.3
Stopcock housing with precision Boro 3.3 burette tip.
Interchangeable borosilicate glass stopcock key with retention device.
Stopcock size 3NS/12. Pack of 1.
For burette capacity Boro 3.3 Boro 3.3
ml Lateral stopcock Straight stopcock
Cat. No. Cat. No.
2 - 10 818 05 818 15
25 818 07 818 17
50 818 09 818 19
2 - 10 811 40 810 53
25 811 41 810 53
50 811 42 810 53
214 info@brand.de
Volumetric Instruments
BRAND online
Volumetric Instruments
Notes:
www.brand.de 215
© Mark Newman/Lonely Planet Images/Getty Images
Temperature
and Density
Measurement
For the measurement of temperature and density,
BRAND offers high quality thermometers, individually
calibrated BLAUBRAND® density bottles, and fast,
reliable hydrometers.
Density Bottles
Density Bottles
Temperature and Density
Density bottles,
calibrated
BLAUBRAND®
Borosilicate glass 3.3. DIN ISO 3507, Nominal capacity Cat. No.
Gay-Lussac type. Calibrated to contain cm³
(TC, In). Reference temperature 20 °C.
5 433 05
Individual certificate included. Stopper
10 433 08
NS 10/19 with capillary. Top of stopper
25 433 20
ground and polished. The volume in cm3
50 433 28
is specified to a precision of 3 decimal
100 433 38
places. Pack of 1.
Inscriptions in high
contrast blue enamel
Density bottles,
calibrated
BLAUBRAND®. No longer available in EU
With thermometer and side capillary Member States (EU direc-
Borosilicate glass 3.3. DIN ISO 3507. tive EG 847/2012).
Calibrated to contain (TC, In). Reference
temperature 20 °C. Individual certificate
included. Side capillary with conical ground Nominal capacity Cat. No.
cap size NS 7/16. Thermometer with cm³
enclosed scale, with standard ground joint
10 434 08
NS 10/19, range 10 to 35 °C, divided in
25 434 20
0.2 °C, mercury filled. The volume in cm3
50 434 28
is specified to a precision of 3 decimal
places. Pack of 1. 100 434 38
218 info@brand.de
Oxygen flasks
uncalibrated cm³
5 432 05
Borosilicate glass 3.3. DIN ISO 3507,
10 432 08
Gay-Lussac type. Stopper NS 10/19
25 432 20
with capillary. Top of stopper ground and
50 432 28
polished. Nominal capacity printed on the
100 432 38
bottom. Pack of 2.
Oxygen flasks,
Winkler pattern
Soda-lime glass. For the determination of Each flask and its stopper is marked with a
oxygen dissolved in water. The measured unique matching identification number.
volume is indicated to a precision of 2 Pack of 2.
decimal places. White labelling area. Solid,
obliquely cut standard-ground glass stopper Nominal capacity Neck Size Cat. No.
can be secured with a fastening clip. ml
Each flask is calibrated with its own stop-
100 - 150 14/23 3860 38
per; hence, stoppers and flasks are not
250 - 300 19/26 3860 48
interchangeable.
3860 38 3861 38
3860 48 3861 48
www.brand.de 219
Centrifuge Tubes
220 info@brand.de
Sedimentation Cones
Sedimentation Cones
Imhoff
sedimentation cones
SILBERBRAND. Graduated to
100 ml, ring mark at 1000 ml.
Borosilicate glass 3.3. DIN 12 672.
Graduation range Subdiv. Error limit
ml ml ± ml
0 - 2 0.1 0.1
> 2 - 10 0.5 0.5
With stopcock. Without stopcock.
> 10 - 40 1 1
Pack of 1. Pack of 4.
> 40 - 100 2 2
Ring mark 1000 – 10 Cat. No. 3876 62 Cat. No. 3873 62
www.brand.de 221
Sedimentation Cones
Temperature and Density
0 - 2 0.1 0.1
> 2 - 10 0.5 0.5
> 10 - 40 1 1
> 40 - 100 2 2
> 100 - 1000 50 10
0 - 2 0.1 0.1
> 2 - 10 0.5 0.5
> 10 - 40 1 1
> 40 - 100 2 2
> 100 - 1000 50 10
222 info@brand.de
Racks for
Sedimentation Cones
www.brand.de 223
Hydrometers
Temperature and Density
Hydrometers
Measuring Procedures
224 info@brand.de
Hydrometers
www.brand.de 225
Hydrometers
Without thermometer:
Subdivision 0.002 g/cm3, refer-
ence temperature 20 °C.
Approx. 280 mm long.
Pack of 1.
With thermometer:
Thermometer range:
0-30/40 °C (subdivision 1 °C).
Petroleum filled, blue colored. Without thermometer.
Approx. 330 mm long. Reference temperature 20 °C.
Pack of 1. Pack of 1.
Range without thermometer with thermometer Range Subdivisions Length Cat. No.
g/cm³ Cat. No. Cat. No. °Bé °Bé approx. mm
226 info@brand.de
Hydrometers
www.brand.de 227
Hydrometers
Temperature and Density
Hydrometer Hydrometer
cylinders cylinders
Special-purpose
hydrometers
Reference temperature 20 °C.
Without thermometer, approx.
290-320 mm long.
All instruments listed below are
supplied with double scales
(weight % and density), elimi-
nating the need for cross ref-
erence documentation.
Pack of 1.
228 info@brand.de
Thermometers
Thermometer
www.brand.de 229
Thermometers
1 Solid-stem thermometers
Temperature and Density
1 2 3
DIN 12770, for general purpose, SILBERBRAND
Calibrated for total immersion. Stem Ø 6-7 mm, yellow coated, upper end round.
Graduation and inscriptions in dark amber stain. Measuring capillary of oval cross section
improves readability. Pack of 1.
Range Subdivision Overall length Filling Cat. No.
from / to °C °C mm
No longer available in
2 Stirring thermometers, solid-stem
EU Member States (EU DIN 12770, for general purpose, SILBERBRAND
directive EG 847/2012). Calibrated for total immersion. With reinforced bottom end for stirring in beakers, etc.
Stem Ø 6-7 mm, yellow coated, upper end round. Graduation and inscriptions in dark
amber stain. Measuring capillary of oval cross section for better readability. Mercury filled.
Pack of 1.
Range Subdivision Overall length Cat. No.
from / to °C °C mm
3 Enclosed-scale thermometers
DIN 12770, for general purpose, SILBERBRAND
Calibrated for total immersion. Tube Ø 7-8 mm, upper end with ring. Opal glass scale
with black graduation and inscriptions. Prismatic measuring capillary, in brilliant blue.
Mercury filled. Pack of 1.
Range Subdivision Overall length Cat. No.
from / to °C °C mm
230 info@brand.de
Thermometers
No longer available in
EU Member States (EU
directive EG 847/2012).
-30 / 50 1 8385 01
0 / 100 1 8385 03
www.brand.de 231
Thermometers
1 2,3
DIN 12 770, GOLDBRAND
Calibrated for total immersion. Stem Ø 6-7 mm, yellow coated, top end round.
Black graduation and inscriptions. Measuring capillary with oval cross section for
improved readability. Mercury filled. Pack of 1.
Range Subdivision Overall length Suitable for official certification*
from / to °C °C mm Cat. No.
232 info@brand.de
Thermometers
www.brand.de 233
Thermometers
Temperature and Density
1 2 3
234 info@brand.de
Thermometers
ASTM thermometers
121 C T
98.6 / 101.4 0.05 305 total immers. 8800 85
* upon request, officially calibrated with calibration certificate and/or DAkkS calibration certificate,
** upon request, available officially tested with calibration certificate and/or DAkkS calibration certificate.
W
Beaded type, T Thermometer with auxiliary scale at 0 °C
www.brand.de 235
Thermometers
Temperature and Density
No longer available in
EU Member States (EU
directive EG 847/2012).
236 info@brand.de
Temperature and Density
BRAND online
www.brand.de 237
© Ann Cutting/Workbook Stock/Getty Images
Clinical
Laboratory
Clinical laboratories see a wide range of specimens
and use a great variety of methods.
Products from BRAND for the medical laboratory are
subjected to rigid quality control procedures. This
ensures precise results.
Sample Cups
Sample cup
Clinical Laboratory
for Technicon-Analyzer
PS, transparent. CE-marked according to IVD-Directive 98/79 EC.
Packed in bags of 1000.
Capacity Upper Ø Lower Ø Height Cat. No.
ml mm mm mm
Push-on caps
for Analyzer Sample cup
PE. Suitable for Technicon 1.5 ml and 2 ml sample tubes.
CE-marked according to IVD-Directive 98/79 EC.
Pack of 1000.
Cat. No. 1150 20
Sample cup
with snap cap
PS, transparent. PE snap cap.
Pack quantity: 1000 = 10 bags of 100.
Capacity Ø Height Cat. No.
ml mm mm
12 22 38 7220 60
Sample cup
for COULTER COUNTER®
PS, transparent. PE lid. CE-marked according to IVD-Directive 98/79 EC.
Pack quantity: 1000 = 4 bags of 250.
Capacity Ø Height Cat. No.
ml mm mm
20 32 56 7220 55
240 info@brand.de
Jars
Clinical Laboratory
Jar with snap-on lid
Jar PS, snap-on lid PE-LD. Conical shape. Pack of 1000.
Capacity max. Ø Height Cat. No.
ml mm mm
25 47 32 623 15
approx. 50 56 25 618 15
approx. 115 75 30 618 20
30 57 32 623 10
www.brand.de 241
Containers
Clinical Laboratory
Urine beaker
with press-on lid
PP, with red PE lid. For hygienic and odorless urine sample handling. Lid is simply sealed
by being pressed on. Easy to use: simply cut off integrated spout tip and insert test strip.
CE-marked according to IVD-Directive 98/79 EC.
Description Capacity Subdivision Ø Height Pack Cat. No.
ml ml mm mm of
Urine beaker
with screw cap
PP, with PE screw cap. For hygienic and odorless urine sample handling.
CE-marked according to IVD-Directive 98/79 EC.
Description Capacity Subdivision Ø Height Pack Cat. No.
ml ml mm mm of
Faeces container
with screw cap
PS. With label. Easy to use with PS screw cap which serves as a sample scoop grip.
CE-marked according to IVD-Directive 98/79 EC. Pack of 400.
Description Capacity Ø Height Cat. No.
ml mm mm
50 19 617 50
242 info@brand.de
Disposal Bags · Containers
Clinical Laboratory
Disposal bags
for collection of disposable items after use
PP/PA. Caution: Risk of injury! Never put sharp objects such as
needles into plastic bags. Bags must be open during sterilization.
PP, autoclavable (121 °C).
PA, autoclavable (134 °C) and hot-air sterilization at 180 °C.
Description Length Width Pack Cat. No.
mm mm of
www.brand.de 243
Petri Dishes
Inoculation Loops
Petri dish
Clinical Laboratory
Soda-lime glass. Excellent glass quality and workmanship. Dish and lid are flat inside and
outside and are free from bubbles and streaks. Cut edges are fire-polished.
Pack of 10.
Lid Ø Dish height (base) Cat. No.
mm mm
40 12 4557 01
60 15 4557 17
80 15 4557 32
100 15 4557 42
100 20 4557 43
150 25 4557 51
Petri dish
PS. Transparent, with lid, disposable. Available with or without vent lid.
Dishes and lids are assembled robotically to reduce the risk of contamination.
Pack of 480 = 24 bags of 20.
Description Lid Ø Height Cat. No.
mm mm
Petri dish
PS. With or without vent. Dishes and lids are assembled robotically to reduce the risk
of contamination. Pack of 1620 = 108 bags of 15.
Description Lid Ø Height Cat. No.
mm mm
Inoculation loops
with needle, disposable
PS. For inoculation of nutrient media. Available either with loop at one end and needle at
the other or loops at both ends. High flexibility permits gentle streaking without damaging
the nutrient surface. γ-ray sterilized. Pack quantity: 1000 = 50 bags of 20.
Description Color Cat. No.
244 info@brand.de
Culture Tubes
Centrifuge Tubes
Culture tubes
Clinical Laboratory
Rimless or with screw cap (GL thread)
AR-GLAS®. Screw cap, PP, with white TPE elastomer seal.
Autoclavable at 121 °C (2 bar), acc. DIN EN 285.
Description Capacity Outer-Ø Height Wall thick- RCF Pack Cat. No.
ml mm mm ness, mm max. of
Centrifuge tubes
Rimless, round bottom
PC. Embossed graduation, subdivisions approx. 1 mm. Transparent, slight yellow tint.
Withstands RCF up to 20 000. Pack of 10.
Capacity Subdivision Outer-Ø Height Cat. No.
ml ml mm mm
50 1 35 99 7810 29
100 – 41 115 7810 39
100 2 45 98 7810 40
Centrifuge tubes
with screw cap
PP, translucent. Withstands RCF up to 3000. Graduated, available non-sterile and
γ-ray sterilized in 15 ml or 50 ml. Screw cap PE with sealing cone.
CE-marked according to IVD-Directive 98/79 EC.
Capacity Description g-ray sterile/ Outer-Ø Height Pack Cat. No.
ml non-sterile mm mm of
www.brand.de 245
Centrifuge Tubes
Clinical Laboratory
Centrifuge tubes
Cylindrical, with rim
PP. Without stopper. Withstands RCF up to 4500. Autoclavable (121 °C).
Capacity Outer-Ø Height Pack of Cat. No.
ml mm mm (without stopper)
PE-Stopper *
for PP centrifuge tubes
PE. Packed in bags of 100.
Suitable for tube Pack Cat. No.
No. of
246 info@brand.de
Tubes
Clinical Laboratory
Sample tubes
PS, transparent.
Type of RCF Outer-Ø Height Pack Cat. No.
tube max. mm mm of
Grip stopper
for sample tubes
PE-LD, neutral. Packed in bags of 1000.
Stoppers Pack Cat. No.
for tubes of
Sedimentation tube
PS, transparent. Conical bottom. Pack of 2000 = 2 bags of 1000.
RCF Outer-Ø Height Cat. No.
max. mm mm
www.brand.de 247
Test Tube Racks
Clinical Laboratory
13 21 180 60 60 1155 10
19 10 180 60 70 1155 15
30 4 180 60 80 1155 20
248 info@brand.de
Pasteur Pipettes
Dropping Pipettes
Clinical Laboratory
Pasteur pipettes
disposable
PE-LD. Very good reproducibility of the number of drops per milliliter. Ideal for aliquots.
The integrated pipetting bulb depresses easily, minimizing fatigue from frequent pipetting.
Resistant to gas or gamma radiation sterilization. Pack of 500.
Graduation Withdraw Outer-Ø Length Drop Cat. No.
ml volume tip mm quantity
ml mm of ml
Dropping pipettes
with integrated bellows
PE-LD. For sampling and decanting infectious or toxic liquids. Graduated. Pack of 100.
Capacity Length Cat. No.
ml mm
Dropping pipette
with integrated pipetting bulb
PE-LD. Pack of 250.
Capacity Length Cat. No.
ml mm
1.8 98 1254 00
www.brand.de 249
Pasteur Pipettes · Rubber Cap
Clinical Laboratory
Pasteur pipettes
Soda-lime glass. ISO 7712. Long drawn-out capillary tip. Suction end with constriction
for a cotton plug. Pack of 1000 = 4 boxes of 250.
Capacity Inner-Ø Outer-Ø Length Length Total Cat. No.
ml tip mm suction tip length
mm tube, mm mm mm
Rubber cap
Natural rubber (NR). Pipetting aid for
glass pasteur pipettes. Pack of 100.
Cat. No. 1247 00
250 info@brand.de
Disposable Micropipettes
Clinical Laboratory
Disposable micropipettes, intraMARK
BLAUBRAND®, DE-M marking, with ring mark
DIN ISO 7550. Volumes greater than 5 µl are with DE-M marking.
Calibrated to contain (TC, In). Color code to ISO for clear identification.
CE-marked according to IVD-Directive 98/79 EC.
Pack quantity 1000 = 4 boxes of 250. 200 µl: Pack of 800 = 4 boxes of 200.
Mark(s) Color Code Length Accuracy Reproducibility Cat. No.
at µl mm ≤±% ≤%
www.brand.de 251
Pipetting aid
Micro Haematocrit
Clinical Laboratory
Pipetting aid
For BLAUBRAND® intraEND micropipettes
(> 1 µl). Consisting of the PET plastic tube,
silicone adapter, and TPE suction bellows
with venting hole. Pack of 10.
Cat. No. 7091 10
Pipette holder
For intraEND and Delbrück micropipettes
and test tubes (EASYCAL™).
Pack of 10.
Cat. No. 7086 05
heparinized red 75 ± 0.5 0.2 ± 0.025 1.15 ± 0.05 1.55 ± 0.05 7493 11
non-heparinized blue 75 ± 0.5 0.2 ± 0.025 1.15 ± 0.05 1.55 ± 0.05 7493 21
Note! Hold the capillaries (~ 2/3 full) near the empty end,
and stick the empty end into the cement with a slight
twist to seal it.
252 info@brand.de
Counting Chambers
Counting Chambers
Clinical Laboratory
Counting chambers serve to determine the number of particles per volume unit of a liquid.
The particles (e.g., leucocytes, erythrocytes, thrombocytes, bacteria, fungus spores, pollen)
are visually counted under a microscope. BLAUBRAND® counting chambers are precision
measuring instruments.
Counted particles
Particles per µl volume =
Counted surface (mm2) · Chamber depth (mm) · Dilution
Example: Erythrocytes
Chamber: Improved Neubauer
528 · 200
1. Counted particles: 528 erythrocytes
0.2 · 0.1 · 1
2. Counted surface: 5 group squares,
equivalent to 0.2 mm2 = 5.28 · 10 6 ery/µl blood
3. Chamber depth: 0.1 mm
4. Dilution: 1:200 = 5.28 Mio ery/µl blood
Cleaning
For cleaning, we recommend the disinfectant cleaner Mucocit®-T (page 313).
www.brand.de 253
Counting Chambers
Improved Neubauer
Clinical Laboratory
Neubauer
double ruling, with or without spring clips
The ruling shows 9 large squares of 1 mm2 each.
The four large squares in the corners marked "L" for
counting leucocytes are each subdivided into 16 squares
with 0.25 mm sides.
The large square in the center is subdivided into 16 group
squares of 0.2 mm sides. Each group square consists of
16 mini squares with 0.05 mm sides, each having an area
of 0.0025 mm2.
The 5 group squares marked "E" are used for counting
Large central square
thrombocytes and erythrocytes.
Contrary to the more advanced Improved Neubauer counting
Description Chamber depth Cat. No. chambers, the counting area of each group square is limited
by the outer one of the triple boundary lines. CE-marked
without spring clips 0.1 mm 7186 05 according to IVD-Directive 98/79 EC.
with spring clips 0.1 mm 7186 20
254 info@brand.de
Counting Chambers
Thoma
Clinical Laboratory
double ruling, with or without spring clips
Rulings correspond to the central large square of the Neubauer
chamber. The mini squares have an area of 0.0025 mm2 each.
Since the outer large squares are not completed, the Thoma
chamber is only used for counting thrombocytes and erythro-
cytes. CE-marked according to IVD-Directive 98/79 EC.
Bürker
double ruling, with or without spring clips
The ruling shows 9 large squares of 1 mm2 each. These are
used for counting leucocytes. Each large square is subdivided
by double lines (0.05 mm apart) into 16 group squares with
0.2 mm sides. The group squares correspond in size to the
Neubauer counting chamber, but have no further subdivisions.
They are used for counting thrombocytes and erythrocytes.
The double lines form mini squares with an area of
0.0025 mm2.
CE-marked according to IVD-Directive 98/79 EC.
Large central square
Bürker-Türk
double ruling, with or without spring clips
Combination of the Bürker and Thoma systems. The chamber
depth is 0.1 mm. The ruling shows 9 large squares of 1 mm2
each. The large squares are subdivided into 16 group squares
with 0.2 mm sides. In the central large square, each group
square is subdivided into 16 mini squares with 0.05 mm sides
(= 0.0025 mm2). CE-marked according to IVD-Directive
98/79 EC.
www.brand.de 255
Counting Chambers
Fuchs-Rosenthal
Clinical Laboratory
Large square
Malassez
double ruling, without spring clips
The ruling is rectangular, covering an area of 5 mm2.
The large rectangles measure 0.25 x 0.20 = 0.05 mm2.
They are each subdivided into 20 mini squares with an area
of 0.0025 mm2. This chamber is commonly used for counting
cells in the cerebrospinal fluid, or for counting nematodes.
CE-marked according to IVD-Directive 98/79 EC.
Large square
Nageotte
double ruling, without spring clips
The chamber depth is 0.5 mm. The square area of
100 mm2 is subdivided into 40 rectangles with an area of
0.25 x 10 = 2.5 mm2 each. This chamber is commonly used
for counting cells in the cerebrospinal fluid, or for counting
nematodes. CE-marked according to IVD-Directive 98/79 EC.
256 info@brand.de
Haematology · Microscopy
Clinical Laboratory
Haemacytometer cover glasses
for counting chambers
Pure white (clear) borosilicate glass, hydrolytic class 1, DIN ISO 8255.
Refractive index ne = 1.52 ± 0.01; Abbe number ve = 56.5 ± 0.5. Flatness tolerance
± 3 µm. Haemacytometer cover glasses differ from ordinary cover glasses by their plain
ground and polished surface. CE-marked according to IVD-Directive 98/79 EC.
Packing: 2 cover glasses in a plastic bag, separated by tissue paper.
Pack quantity: 10 boxes at 10 cover glasses = 100 cover glasses per carton.
Length Width Thickness Cat. No.
mm mm mm
24 24 0.4 7230 14
20 26 0.4 7230 15
22 30 0.4 7230 16
Microscope slides
White (clear) soda-lime glass, hydrolytic class 3. Thickness approx. 1 mm, size approx.
76 x 26 mm (DIN ISO 8037-1). We recommend using slides with ground edges to
reduce the risk of injury. To protect against penetrating humidity the entire packing unit
is also optionally available sealed in an aluminium bag.
Pack quantity 2500 = 50 boxes of 50.
Description Cat. No.
www.brand.de 257
Haematology · Microscopy
Clinical Laboratory
Cavity slides
White (clear) soda-lime glass, hydrolytic class 3. Rectangular ground edges.
Thickness 1.2 - 1.5 mm, size in mm: 76 x 26. Concavities of 15-18 mm Ø,
depth 0.6 - 0.8 mm. Pack of 50.
Description Cat. No.
1 concavity 4755 05
2 concavities 4755 35
3 concavities 4755 65
258 info@brand.de
Microscopy
Clinical Laboratory
Staining trough, Coplin
Soda-lime glass. For 10 slides, size in mm: 76 x 26. Pack of 10.
Description Ø Height Cat. No.
mm mm
Spotting tile
Soda-lime glass. 12 polished cavities 20-22 mm Ø, depth 2 mm,
capacity approx. 0.2 ml. Ground rectangular edges. Pack of 1.
Description Length Width Height Cat. No.
mm mm mm
www.brand.de 259
Microscopy
Clinical Laboratory
Slide boxes
PS. For slides, size (in mm) 76 x 26. Numbered slots in base.
Lid with index card. Pack of 1.
For ... slides Length Width Height Cat. No.
mm mm mm
25 120 96 35 4758 00
50 230 97 35 4759 00
100 230 187 35 4760 00
Slide box
PP, sturdy design. Screw lid with sealing wire lug. Ideal for storing or shipping 5 thick or
10 thin slides. Slides protrude 10 mm above the box, to facilitate removal. Pack of 10.
Description Cat. No.
260 info@brand.de
Clinical Laboratory
BRAND online
Clinical Laboratory
Notes:
www.brand.de 261
© Thomas Dressler/Gallo Images/Getty Images
General
Laboratory
Supplies
Sample preparation and storage impose a wide variety
of requirements on laboratory instruments. Depending
on the requirements, we offer materials ranging from
glass to specialty plastics, like PFA for trace analysis.
Sealing Film
50 75 24 7016 11
100 38 12 7016 05
100 75 12 7016 06
500 15 6 7015 01
➀ ➁
PARAFILM ® M Cutter
The handy PARAFILM® M Cutter is
convenient for clean storage and cutting
of PARAFILM® M sealing film.
Suitable for 50 mm and 100 mm wide rolls.
Pack of 1.
264 info@brand.de
Erlenmeyer Flasks · Beakers
General Laboratory
sents the internationally designated
Narrow neck borosilicate glass type 3.3.
Boro 3.3. DIN ISO 1773. With beaded rim and graduation.
Pack of 10 (3000 ml: Pack of 2; 5000 ml: Pack of 1).
Capacity Neck outer-Ø Flask outer-Ø Height Cat. No.
ml mm mm max. mm
25 22 42 75 927 14
50 22 51 90 927 17
100 22 64 105 927 24
200* 34 79 131 927 32
250 34 85 145 927 36
300* 34 87 156 927 39
500 34 105 180 927 44
1000 42 131 220 927 54
2000 50 166 280 927 63
3000 52 187 310 927 68
5000 52 220 365 927 73
* in addition to the ISO range
Wide neck
Boro 3.3. DIN EN ISO 24450. With beaded rim and graduation. Pack of 10.
Capacity Neck outer-Ø Flask outer-Ø Height Cat. No.
ml mm mm max. mm
25* 31 43 70 928 14
50 34 51 85 928 17
100 34 64 105 928 24
200* 50 79 131 928 32
250 50 85 140 928 36
300* 50 87 156 928 39
500 50 105 175 928 44
1000 50 131 220 928 54
2000* 72 153 276 928 63
* in addition to the ISO range
Beakers
Low form Tall form
Boro 3.3. DIN 12 331/ISO 3819. Boro 3.3. DIN 12 331/ISO 3819.
With graduation and spout. With graduation and spout.
Pack of 10 Pack of 10
(3000 ml: Pack of 4; 5000 ml: Pack of 1). (3000 ml: Pack of 2).
Capacity Ø x Height Cat. No. Capacity Ø x Height Cat. No.
ml mm ml mm
5* 22 x 30 912 10 50 38 x 70 906 17
10* 26 x 35 912 12 100 48 x 80 906 24
25 34 x 50 912 14 150 54 x 95 906 29
50 42 x 60 912 17 250 60 x 120 906 36
100 50 x 70 912 24 400 70 x 130 906 41
150 60 x 80 912 29 600 80 x 150 906 48
250 70 x 95 912 36 800 90 x 175 906 53
400 80 x 110 912 41 1000 95 x 180 906 54
600 90 x 125 912 48 2000 120 x 240 906 63
800 100 x 135 912 53 3000 135 x 280 906 68
1000 105 x 145 912 54
2000 132 x 185 912 63
3000 152 x 210 912 68
5000 170 x 270 912 73
* without graduation
www.brand.de 265
Beakers
NEW!
PP, high clarity
Capacity Subdivision Height max. Ø Pack of Blue scale Embossed scale
ml ml mm mm Cat. No. Cat. No.
10* 2 36 30 12 876 02 –
25 5 50 38 12 876 04 878 04
50 10 60 47 12 876 06 878 06
100 10 70 55 12 876 08 878 08
150* 20 80 66 12 876 10 878 10
250 25 95 77 6 876 12 878 12
400* 50 112 87 6 876 14 878 14
500 50 118 94 6 876 16 878 16
600* 50 127 100 6 876 18 878 18
1000 100 147 120 6 876 20 878 20
2000 200 187 149 6 876 22 878 22
3000* 250 212 170 4 876 24 878 24
5000 500 247 203 4 876 26 878 26
* in addition to ISO 7056
NEW!
PMP, transparent
Capacity Subdivision Height max. Ø Pack of Blue scale Embossed scale
ml ml mm mm Cat. No. Cat. No.
10* 2 36 30 12 875 02 –
25 5 50 38 12 875 04 877 04
50 10 60 47 12 875 06 877 06
100 10 70 55 12 875 08 877 08
150* 20 80 66 12 875 10 877 10
250 25 95 77 6 875 12 877 12
400* 50 112 87 6 875 14 877 14
500 50 118 94 6 875 16 877 16
600* 50 127 100 6 875 18 877 18
1000 100 147 120 6 875 20 877 20
2000 200 187 149 6 875 22 877 22
3000* 250 212 170 4 875 24 877 24
5000 500 247 203 4 875 26 877 26
* in addition to ISO 7056
266 info@brand.de
Beakers
General Laboratory
with blue printed scale or embossed
PP, high clarity. Ergonomic handle for secure grip. Functional spout to minimize spills.
Cleaning at temperatures below 60 °C is recommended to preserve marks and inscriptions.
For autoclaving (121 °C), beakers with an embossed scale should be selected.
NEW!
PP, high clarity
Capacity Subdivision Height max. Ø Pack of Blue scale Embossed scale
ml ml mm mm Cat. No. Cat. No.
50 2 70 40 24 408 02 410 02
100 2 80 50 24 408 04 410 04
250 5 120 74 12 408 06 410 06
500 10 140 92 12 408 08 410 08
1000 20 181 117 6 408 10 410 10
2000 50 213 152 6 408 12 410 12
3000 50 242 172 6 408 14 410 14
5000 100 270 204 6 408 16 410 16
25 5 50 32 902 24
50 10 59 39 902 28
100 20 72 50 902 38
250 50 96 67 902 48
400 50 109 77 902 52
500 100 122 88 902 54
600 100 125 91 902 56
1000 100 143 105 902 62
25 5 50 32 901 20
50 10 59 39 901 28
100 20 72 50 901 38
250 50 96 67 901 48
500 100 122 88 901 54
1000 100 141 109 901 62
www.brand.de 267
Beakers · Erlenmeyer Flasks
General Laboratory
5 20 26 903 05
10 24 33 903 08
25 32 47 903 20
50 43 55 903 28
100 54 68 903 38
150 59 75 903 44
250 66 97 903 48
400 80 125 903 52
500 100 125 903 54
1000 105 155 903 62
2000 125 205 903 64
Sampling dippers
PTFE. With reinforced rim and spout. PTFE handle with steel core.
Optional extension rod for increased handle length. Pack of 1.
(Extension rod, length 600 mm. Pack of 1. Cat. No. 904 70)
Capacity Ø Height Cat. No.
ml mm mm
100 55 65 904 38
250 70 95 904 48
500 80 125 904 54
1000 105 155 904 62
50 GL 40 52 90 34/35 931 70
100 GL 40 64 110 34/35 931 72
250 GL 52 85 140 45/40 931 75
500 GL 52 108 180 45/40 931 80
1000 GL 52 135 220 45/40 931 85
268 info@brand.de
Watch Glasses
Evaporating Dishes
Watch glasses
General Laboratory
Ø Cat. No.
mm
Soda-lime glass. DIN 12 341.
Fire-polished edges. Stress-reduced 40 1500 10
to minimize risk of shattering. 50 1500 15
Pack of 10. 60 1500 20
70 1500 30
80 1500 40
90 1500 45
100 1500 50
120 1500 60
125 1500 65
150 1500 70
200 1500 80
Watch glasses Ø
mm
for PTFE beakers
size ml
Cat. No.
www.brand.de 269
Evaporating dishes
Evaporating dishes
General Laboratory
Capacity
ml
max. Ø
mm
Height
mm
Cat. No.
Evaporating dishes
PTFE. Conical with spout.
25 40 35 456 15 Pack of 1.
50 50 40 456 17
100 65 55 456 20
150 80 40 456 21
180 80 50 456 22
250 95 45 456 23
350 100 60 456 25
Capacity
ml
max. Ø
mm
Height
mm
Cat. No.
Crystallizing dishes
PTFE. Cylindrical without spout.
25 40 25 457 03 Pack of 1.
50 65 25 457 05
100 80 30 457 10
180 80 45 457 18
350 100 55 457 22
max. Ø
mm
Height
mm
Cat. No.
Mortars with pestle
Melamine formaldehyde (MF), white.
125 80 1107 05 Resistant to boiling and autoclavable
150 90 1107 10 (121 °C). With foot and spout.
Pack of 1.
270 info@brand.de
Centrifuge tubes · Test tubes
General Laboratory
Centrifuge tubes, ungraduated
AR-GLAS® or Boro 3.3. Wall thickness approx. 1.0 mm. Withstands RCF up to 3500.
Description Glass Capacity Outer-Ø Height Pack Cat. No.
ml mm mm of
0 - 15 AR-GLAS ®
0.1 17 113 ca. 1.0 3500 7787 12
0 - 15 Boro 3.3 0.1 17 113 ca. 1.0 3500 7787 13
Standard ground joint neck with PP stopper 10 0.1 12/21 15 165 1145 08
Standard ground joint neck with PP stopper 20 0.2 14/23 17 200 1145 16
Standard ground joint neck with PP stopper 25 0.2 14/23 17 220 1145 21
Standard ground joint neck with PP stopper 30 0.5 14/23 19 220 1145 24
Sample tubes
with screw cap
PFA-screw cap with integrated seal lip. Individually calibrated ring mark at 10 ml.
Autoclavable, easy to clean, withstands temperatures from -200 °C to +260 °C.
Pack of 1. (Replacement screw-cap, PFA. Pack of 1. Cat. No. 1292 52)
Capacity Ø Height Thread Cat. No.
ml mm mm GL
15 22 110 25 7794 20
www.brand.de 271
Funnels
Funnels
General Laboratory
plain interior
Boro 3.3. Angled at 60°, ground and fire-polished upper rim.
Short stem. Pack of 1.
Funnel outer-Ø Stem outer-Ø Stem length Cat. No.
mm mm mm
35 6 35 1455 05
40 6 40 1455 07
50 7 50 1455 15
55 8 55 1455 20
60 8 60 1455 25
70 8 70 1455 30
80 9 80 1455 35
100 10 100 1455 40
120 16 120 1455 45
150 16 150 1455 50
200 26 175 1455 60
Funnels
fluted interior
Boro 3.3. For rapid filtration. Angled at 60°, ground and fire-polished upper rim. Short
sterm. Pack of 10 (Cat. No. 1456 38: Pack of 1).
Funnel outer-Ø Stem outer-Ø Stem length Cat. No.
mm mm mm
70 8 70 1456 11
80 10 80 1456 16
100 10 100 1456 19
150 16 150 1456 31
200 26 175 1456 38
Funnels
plain interior
PP.
Funnel outer-Ø Stem outer-Ø Stem length Pack of Cat. No.
mm mm mm
30 2 25 24 1470 00
30 5 25 24 1470 05
40 5 35 24 1470 10
50 9 43 24 1470 15
75 10 55 12 1470 20
100 13 77 12 1470 25
120 14 90 12 1470 30
150 17 95 12 1470 35
272 info@brand.de
Funnels
General Laboratory
Funnels
fluted interior
PP. For rapid filtration. Angled at 60°. External flutings allow air to escape during filtration.
Funnel outer-Ø Stem outer-Ø Stem length Pack of Cat. No.
mm mm mm
25 4 39 20 1482 05
35 5 38 20 1482 10
45 5 44 20 1482 15
65 9 63 20 1482 20
80 10 69 20 1482 25
100 11 82 1 1482 35
120 11 85 1 1482 37
150 14 115 1 1482 40
180 14 143 1 1482 45
Powder funnels
Boro 3.3. Ground and fire-polished upper rim. With short, wide stem. Pack of 1.
Upper Ø Lower Ø Cat. No.
mm mm
50 18 1465 06
60 18 1465 08
70 20 1465 11
80 22 1465 16
90 25 1465 17
100 25 1465 19
120 30 1465 23
Powder funnels
PP. With short, wide stem.
Funnel outer-Ø Stem outer-Ø Stem length Pack of Cat. No.
mm mm mm
60 15 19 20 1480 20
80 15 23 20 1480 25
100 25 23 20 1480 30
120 30 28 1 1480 35
150 35 42 1 1480 40
180 40 48 1 1480 45
Carboy funnels
PP. Pack of 1.
Funnel outer-Ø Stem outer-Ø Stem length Cat. No.
mm mm mm
210 25 90 1470 40
260 30 100 1470 45
350 32 170 1470 50
400 40 150 1470 55
440 (PE-HD) 35 140 1470 60
www.brand.de 273
Funnels
General Laboratory
40 14/23 949 05
50 19/26 949 10
80 29/32 949 15
Buchner funnels
PP. Two detachable pieces facilitate cleaning. Use with suggested filter paper.
Pack of 1.
Nominal size Filter disk Ø for filter paper Ø Cat. No.
mm mm mm
45 45 45 1485 05
55 55 55 1485 10
70 72 70 1485 15
80 82 70 1485 20
90 93 90 1485 25
110 120 110 1485 30
160 160 150 1485 35
240 240 240 1485 40
274 info@brand.de
Separating funnels
General Laboratory
Separating funnels
PP, high clarity for observation of contents.
With PP screw cap and special conical
PTFE stopcock with retention device.
Autoclavable (121 °C). Pack of 1.
Capacity Cat. No.
ml
125 1400 04
250 1400 05
500 1400 06
1000 1400 07
www.brand.de 275
Filter Funnels · Filter Crucibles
Filter funnels
General Laboratory
3D 3 50 35 10 4640 03
3D 4 50 35 10 4640 04
11 D 3 75 45 10 4647 13
11 D 4 75 45 10 4647 14
17 D 3 125 60 10 4654 23
17 D 4 125 60 10 4654 24
25 D 3 500 95 22 4661 33
25 D 4 500 95 22 4661 34
Filter crucibles
Boro 3.3. Pack of 10.
Identification Porosity Capacity Ø Filter disk Cat. No.
Code ml mm
1D 1 30 30 4580 21
1D 2 30 30 4580 22
1D 3 30 30 4580 23
1D 4 30 30 4580 24
2D 1 50 40 4587 31
2D 2 50 40 4587 32
2D 3 50 40 4587 33
2D 4 50 40 4587 34
Filter adapters
Boro 3.3. Suitable for filter crucibles, according to identification code above.
Pack of 10.
Identification Upper Ø Overall length Cat. No.
Code mm mm
1D 41 125 4636 16
2D 50 132 4637 32
276 info@brand.de
Filter Flasks
General Laboratory
Rubber sleeves
Rubber (EPDM). Suitable for filter crucibles, according to identification code.
Pack of 10.
Identification Outer Ø Cat. No.
Code mm
1D 41 4620 26
2D 49 4622 31
Rubber gaskets
Rubber (EPDM). Conical gaskets to place between filter funnel and filter flask.
Pack of 10.
Upper outer-Ø Lower outer-Ø Lower inner-Ø Cat. No.
mm mm mm
22 11 6 4625 12
28 16 9 4626 17
35 20 14 4627 23
42 25 18 4628 27
50 32 22 4629 32
63 43 33 4630 36
71 52 42 4631 39
84 61 50 4632 43
Rubber gasket set. Pack of 1. 4625 00
www.brand.de 277
Desiccators
General Laboratory
Desiccators
with knob lid
Boro 3.3. DIN 12 491. Precisely ground flat flanges. Base ring surface studded to
improve stability and help prevent cracks. Interchangeable lid. Pack of 1.
Nominal size Ø Height Cat. No.
mm mm mm
Desiccators
with socket in the lid
Boro 3.3. DIN 12 491. Precisely ground flat flanges. Base ring surface studded to
improve stability and help prevent cracks. Interchangeable lid. Socket, ground joint neck
size 24/29, with interchangeable stopcock. Pack of 1.
Nominal size Ø Height Cat. No.
mm mm mm
278 info@brand.de
Desiccators
General Laboratory
Desiccators
PC lid. PP base and desiccant tray. Venting stopper with non-return valve in lid. Seal
between lid and base: polychlorobutadiene rubber (CR) O-ring, compressed by vacuum.
Lightweight and easy to handle. Pack of 1.
Nominal size Ø Height Cat. No.
mm mm mm
150 658 20
200 658 22
250 658 24
Venting stopper
PC. Pack of 1.
Cat. No. 658 04
Porcelain PP
www.brand.de 279
Gas Wash Bottles
General Laboratory
100 782 38
250 782 48
500 782 54
1000 782 62
100 781 38
250 781 48
500 781 54
1000 781 62
280 info@brand.de
Gas Wash Bottles
General Laboratory
Wash bottle head with P1 filter disk
for gas wash bottles, Drechsel pattern
Boro 3.3. DIN 12 596. Size 29/32. Length below top of cone 185 mm.
Accommodates all bottle sizes to DIN 12 463. Filter disk outer diameter 25 mm,
porosity P1 (approx. 100-160 µm pore size). Pack of 1.
Cat. No. 782 00
www.brand.de 281
Water jet pumps
Ordering data
Water jet pump includes: Accessories
sleeve nut R 3/4", reducing adapter R 1/2", Reducing adapter R 3/8".
and tubing connector (hose nozzle) of Pack of 1.
10-12 mm outer diameter
Cat. No. 1596 65
Suction line connection:
Detachable hose nozzle of 6-9 mm outer
diameter, with screw cap GL 14. Reducing adapter M 22 x 1
Pack of 1. (thread for screen tap). Pack of 1.
Cat. No. 1596 00 Cat. No. 1596 70
282 info@brand.de
Tubing
General Laboratory
Tubing
PVC. Particularly high-grade material, manufactured to a first-class standard. Good flex-
ibility and chemical resistance make this tubing ideal for use with apparatus assemblies.
Autoclavable (121 °C). Packing unit 20 meters.
inner-Ø outer-Ø Wall thickness Cat. No.
mm mm mm
2 4 1 1432 55
4 6 1 1432 60
5 8 1,5 1432 65
6 9 1,5 1432 70
7 11 2 1432 75
8 12 2 1432 80
10 14 2 1432 85
12 17 2,5 1432 90
Tubing
Silicone (SI). Translucent. Autoclavable (121 °C). Note: limited chemical resistance when
used with concentrated acids. Packing unit 25 meters.
inner-Ø outer-Ø Wall thickness Cat. No.
mm mm mm
2 4 1 1433 52
3 5 1 1433 55
4 6 1 1433 56
4 7 1,5 1433 57
5 8 1,5 1433 58
6 9 1,5 1433 59
6 10 2 1433 60
7 10 1,5 1433 61
8 12 2 1433 62
10 15 2,5 1433 64
www.brand.de 283
Tubing Connectors
284 info@brand.de
Tubing Connectors
General Laboratory
straight (tubing adapter) for tubing Length Cat. No.
inner-Ø mm mm
PP, translucent.
Pack of 10.
3 - 5 / 6 - 10 55 1526 05
3 - 5 / 9 - 13 55 1526 10
6 - 10 / 9 - 13 59 1526 15
T-shape
PP, translucent. Length 1 = Length of straight-through tube,
Length 2 = Length of side arm including Ø of straight-through tube.
Pack of 20 (Cat. No. 1528 20 and 1528 25: Pack of 10).
for tubing inner-Ø Length 1 Length 2 Cat. No.
mm mm mm
3-4 20 15 1528 00
4-5 30 23 1528 03
6-7 40 31 1528 05
8-9 55 39 1528 10
10 - 11 69 47 1528 15
12 - 13 79 53 1528 20
14 - 15 89 60 1528 25
Y-shape
PP, transparent. Length1 = overall length, length 2 = distance between the two arms
from one outer edge to the other.
Pack of 20 (Cat. No. 1529 15 and 1529 20: Pack of 10).
for tubing inner-Ø Length 1 Length 2 Cat. No.
mm mm mm
3 24 14 1529 00
4-5 35 21 1529 03
6-7 54 34 1529 05
8-9 64 40 1529 07
10 - 11 74 47 1529 10
12 - 13 87 53 1529 15
14 - 15 98 60 1529 20
www.brand.de 285
Tubing Connectors
Tubing Connectors
General Laboratory
Plug-in coupling
PP. The two parts fit together with a cone- for tubing Length Cat. No.
and-socket joint. Not suitable for pressure inner-Ø mm mm
applications.
Pack of 25. 6-9 68 1523 00
Non-return valve
PE-HD. For tubing of inner diameter 6-9 mm. Length 80 mm. FKM valve disk not
suitable for pressure applications. Pack of 10.
Cat. No. 1340 02
286 info@brand.de
Stopcocks
Straight-bore stopcocks
General Laboratory
with nozzles
PTFE, valve lever PP. for tubing Bore Cat. No.
Outstanding chemical resistance. inner-Ø mm mm
Two integrated hose nozzles.
Pack of 1. 3 2 886 10
4 2 886 15
5 3 886 20
6 3 886 25
7 4 886 30
8 4 886 35
9 5 886 40
T-bore stopcocks
with nozzles
PTFE, valve lever PP. for tubing T-bore Cat. No.
Outstanding chemical resistance. inner-Ø mm mm
Three integrated hose nozzles.
Pack of 1. 3 2 886 50
4 2 886 55
5 3 886 60
6 3 886 65
7 4 886 70
8 4 886 75
9 5 886 80
Straight-bore stopcocks
with nozzles
PE-HD. For operation under ambient pressure only. Two integrated hose nozzles.
Pack of 1.
for tubing inner-Ø Bore Length Cat. No.
mm mm mm
12.5 9 97 885 05
9.5 7 80 885 10
www.brand.de 287
Conical Joint Clips
Ground Joint Sleeves
Stopcock grease,
silicone-free
Ideal for lubricating burette stopcocks.
Silicone paste
Easy to remove, e.g., with Mucasol®. Low viscosity. Operating temperature
Good acid- and base-resistance. Operating from -40 to +160 °C. For greasing
temperature range -40 to +320 ºC. ground joints.
Tube of 60 g. Pack of 1. Tube of 50 g. Pack of 1.
Cat. No. 616 10 Cat. No. 616 05
288 info@brand.de
Sockets · Stoppers
Sockets
General Laboratory
Size Cat. No.
Rubber stoppers
Natural rubber (NR), red.
upper-Ø lower-Ø Height Pack of Cat. No.
mm mm mm
9 5 20 25 1443 80
12 8 20 25 1443 82
14 11 20 25 1443 84
16 12 20 25 1443 85
18 14 20 25 1443 86
22 17 25 25 1443 88
24 18 30 25 1443 89
27 21 30 25 1443 90
32 26 30 25 1443 91
35 29 30 5 1443 93
38 31 35 5 1443 94
44 36 40 5 1443 95
49 41 40 5 1443 96
55 47 40 1 1443 97
60 50 45 1 1443 98
65 56 45 1 1443 99
Silicone stoppers
SI, natural-colored.
upper-Ø lower-Ø Height Pack of Cat. No.
mm mm mm
9 5 20 10 1443 05
12 8 20 10 1443 10
14 11 20 10 1443 15
16 12 20 10 1443 20
18 14 20 10 1443 25
22 17 25 10 1443 30
24 18 30 10 1443 35
27 21 30 10 1443 40
32 26 30 10 1443 45
35 29 30 10 1443 50
38 31 35 5 1443 55
44 36 40 5 1443 60
49 41 40 5 1443 65
55 47 40 5 1443 70
75 64 55 5 1443 75
www.brand.de 289
Conical Ground Joint Stoppers
290 info@brand.de
Stirrers
Stirrers
General Laboratory
Good operating characteristics due to steel-core shafts. Resistant to breakage.
PP propeller greatly reduces scratching if stirrer inadvertently hits vessel wall.
Pack of 1.
6 380 45 1356 00
Propeller, PTFE
Stirrer shaft Ø Stirrer shaft length Rotor Ø Cat. No
mm mm mm
6 400 40 1342 90
6 500 40 1342 91
9.5 550 60 1342 92
9.5 650 60 1342 93
6 400 50 1342 86
6 500 50 1342 87
9.5 550 70 1342 88
9.5 650 70 1342 89
Anchor, PTFE
Stirrer shaft Ø Stirrer shaft length Rotor Ø Cat. No
mm mm mm
6 500 75 1342 96
9.5 550 100 1342 97
9.5 650 140 1343 00
www.brand.de 291
Magnetic Stirring Bars
PTFE. High magnetic strength and long life due to Alnico V magnetic cores, Storing conditions:
fully encapsulated with high-grade PTFE. Strict quality control ensures the To prevent demagnetization stirring bars us-
magnetic strength, position of the magnetic core, surface quality, crack ing Alnico V magnetic cores should not be
resistance, and uniform thickness of the PTFE coating. Maximum operating stored in a random mass but should be kept
temperature 270 °C. "paired" and should not be dropped on hard
BRAND provides the right magnetic stirring bar for virtually any application. Rough or steel surfaces.
vessel surfaces or abrasive sediments can quickly deteriorate the PTFE coating and
thus shorten the life of stirring bars. This can be prevented by using reduced contact
surface (e.g., with pivot ring) stirring bars.
8 3 4 1374 04
12 4.5 6 1374 05
15 4.5 6 1374 10
20 6 8 1374 15
25 6 7 1374 20
30 6 7.5 1374 25
35 6 8 1374 27
40 8 8.5 1374 30
45 8 10 1374 32
50 8.5 11 1374 35
60 8.5 11 1374 37
70 8.5 10 1374 45
292 info@brand.de
Magnetic Stirring Bars
Cylindrical, conical
General Laboratory
Length Bar Ø Cat. No.
mm mm
PTFE. Round, smooth surface. Diameter
increases toward the center to reduce the
10 4 1371 68
contact surface. Pack of 10.
15 5 1371 70
20 7 1371 75
25 8 1371 78
30 8 1371 80
40 8 1371 85
50 8 1371 90
60 8 1371 95
70 10 1371 97
80 10 1371 99
13 8 10 1376 07
15 8 9.5 1376 08
25 8 9.5 1376 10
25 10 11 1376 12
38 8 9.5 1376 15
38 10 11 1376 17
51 8 9.5 1376 18
51 10 11 1376 20
64 10 11 1376 22
75 12 14.5 1376 25
www.brand.de 293
Magnetic Stirring Bars
Double-ended
PTFE. Excellent centering, small contact surface and high turbulence even at low speeds.
Pack of 10.
Length Bar Ø Ends Ø Cat. No.
mm mm mm
37 8 20 1379 30
54 8 20 1379 35
294 info@brand.de
Magnetic Stirring Bars
Magnetic Stirring Bar Retriever
General Laboratory
Round, with crosshead Ø Height Cat. No.
mm mm
PTFE. Specialshape for small vessels.
Particularly effective stirring action.
10 8 1379 05
Pack of 10.
14 10 1379 10
17 13 1379 15
22 15 1379 17
30 12 1379 19
Magnetic stirring
bar retrievers
PP. Magnetic core fully sealed.
Pack of 1.
Length Ø Cat. No.
mm mm
300 10 1377 50
450 10 1377 55
Magnetic stirring
bar retrievers
PTFE. Magnetic core fully sealed.
Pack of 1.
Length Ø Cat. No.
mm mm
150 8 1377 00
250 8 1377 10
350 8 1377 20
www.brand.de 295
Stirring Rods · Spatulas
General Laboratory
Spatulas
Impact-resistant PS. Available with double spatulate ends or a spatulate
and spoon combination. Pack of 10.
Length Description Cat. No.
mm
260 7 1361 00
Spatula
PP. Two differently shaped spatulate ends (approx. 15 mm wide).
Pack of 10.
Length Ø Cat. No.
mm mm
245 6 1361 10
296 info@brand.de
Forceps · Scoops
Forceps
General Laboratory
Length Cat. No.
mm
PMP. Pointed ends. Autoclavable (121 °C).
Pack of 10. 115 1128 05
145 1128 10
Forceps Length
mm
Cat. No.
Forceps Length
mm
Cat. No.
Forceps Length
mm
Cat. No.
Scoops Capacity
ml
Length
mm
Cat. No.
Scoops Capacity
ml
Length
mm
Cat. No.
www.brand.de 297
Weighing Dishes · Jars
General Laboratory
7 45 45 7 1555 40
100 84 84 24 1555 42
250 140 140 21 1555 44
25 70 45 12 1555 46
30 80 56 14 1555 48
100 120 90 17 1555 50
5 23 34 619 50
10 23 52 619 55
30 37 53 619 60
60 37 92 619 65
90 57 62 619 70
180 57 108 619 75
30 54 38 GL 40 620 05
60 90 38 GL 40 620 10
90 62 54 GL 56 620 15
180 112 54 GL 56 620 20
298 info@brand.de
Bottles
General Laboratory
Laboratory bottles
Boro 3.3. Threaded to DIN ISO 4796. Studded base for stability and abrasion resistance.
Graduated, with marking area. Screw cap and pouring ring PP. Autoclavable (121 °C).
Pack of 10 (5000 ml to 20 000 ml: Pack of 1).
Capacity Thread Ø Height Cat. No.
ml mm mm
25* GL 25 36 74 1225 20
50 GL 32 46 91 1225 28
100 GL 45 56 105 1225 38
250 GL 45 70 144 1225 48
500 GL 45 86 183 1225 54
1000 GL 45 101 232 1225 62
2000 GL 45 136 268 1225 64
5000 GL 45 186 338 1225 70
10000 GL 45 234 412 1225 74
20000 GL 45 300 510 1225 78
* integrated glass ring, no additional pouring ring
Thread Screw Cap Pouring ring Spare parts for laboratory bottles
Cat. No. Cat. No.
PP. Pack of 10.
GL 25 1226 20 –
GL 32 1226 28 1226 62
GL 45 1226 38 1226 64
www.brand.de 299
Bottles
General Laboratory
Narrow-mouth bottles
PE-LD, flexible, translucent. Economical multipurpose bottle.
With or without screw cap.
Capacity Ø Height Thread Pack with cap without cap
ml mm mm of Cat. No. Cat. No.
GL 14 100 1292 00
GL 18 50 1292 02
GL 25 50 1292 04
GL 28 25 1292 06
50 39 85 GL 18 1302 28
100 45 106 GL 18 1302 38
250 59 140 GL 25 1302 48
500 75 180 GL 25 1302 54
1000 94 212 GL 28 1302 62
2000 117 264 GL 28 1302 64
300 info@brand.de
Bottles
Wide-mouth bottles
General Laboratory
PE-LD, flexible, translucent. With or without screw cap.
Capacity Ø Height Thread Pack with cap without cap
ml mm mm of Cat. No. Cat. No.
GL 32 25 1292 08
GL 40 25 1292 10
GL 50 25 1292 12
GL 65 25 1292 14
Wide-mouth bottles
PTFE. Outstanding chemical and thermal resistance. Sturdy, strong-walled design. With
screw cap. Pack of 1.
Capacity Ø Height Neck inner-Ø Cat. No.
ml mm mm mm
5 22 35 10 1305 05
10 26 50 12 1305 08
25 33 61 19 1305 20
50 43 76 25 1305 28
100 52 88 35 1305 38
150 60 90 35 1305 44
250 67 120 42 1305 48
500 80 150 52 1305 54
1000 100 185 57 1305 62
2000 120 240 60 1305 64
www.brand.de 301
Bottles
General Laboratory
Wide-mouth bottles
PFA. High-quality bottles, specially designed for long-term storage of solvents and
standards (trace analysis). PFA screw cap with integrated seal lip and buttress thread.
Autoclavable, easy to clean, temperature resistant from -200 ºC to +260 ºC.
Pack of 1.
Capacity Ø Height Thread Cat. No.
ml mm mm
Narrow-mouth bottles
PFA. High-quality bottles, specially designed for long-term storage of solvents
and standards (trace analysis). PFA screw cap with integrated seal lip and buttress
thread. Autoclavable, easy to clean, temperature resistant from -200 ºC to +260 ºC.
Pack of 1.
Capacity Ø Height Thread Cat. No.
ml mm mm
50 37 86 S 28 1289 28
100 45 120 S 28 1289 38
250 61 160 S 28 1289 48
500 76 190 S 28 1289 54
1000 96 240 S 28 1289 62
Narrow-mouth bottles
Technical quality PFA, ETFE screw caps. These bottles include components made of
reprocessed PFA material from our production process. Physical properties and chemical
resistance equivalent to highly-pure PFA.
Pack of 1.
Capacity Ø Height Thread Cat. No.
ml mm mm
50 37 90 GL 18 1304 80
100 45 114 GL 18 1304 82
250 61 157 GL 25 1304 84
500 76 189 GL 25 1304 86
1000 96 233 GL 32 1304 88
302 info@brand.de
Bottles
Wash bottles
General Laboratory
without venting valve
PE-LD, flexible. Wide-mouth. Capacity 250 ml and 500 ml. High-quality wash bottles
with integrated wash stream and sealing cone closure. Large opening for convenient
filling. Label with designation of contents and general safety statements in English
(with CAS numbers and NFPA codes) and German (with CAS numbers). Pack of 5.
Wash heads
Wash bottles Thread Pack Cat. No.
with interchangeable wash head of
www.brand.de 303
Bottles
General Laboratory
Rubber caps
Natural rubber (NR). Fitting all sizes.
Pack of 100.
Cat. No. 1247 00
Dropping bottles
PE-LD, flexible. Detachable threaded cap with dropper nozzle and attached spout cap.
Capacity Ø Total height Thread Pack Cat. No.
ml mm mm of
20 31 88 GL 14 100 1252 16
30 34 96 GL 14 100 1252 24
50 39 115 GL 18 100 1252 28
100 45 136 GL 18 50 1252 38
250 59 170 GL 25 50 1252 48
500 75 209 GL 25 50 1252 54
1000 94 240 GL 28 25 1252 62
GL 14 100 1292 30
GL 18 50 1292 32
GL 25 50 1292 34
GL 28 25 1292 36
Dropping bottles
PTFE, flexible. Outstanding chemical resistance. Dropper nozzle with screw cap.
Pack of 1.
Capacity Ø Height Cat. No.
ml mm mm
25 33 93 1253 16
50 43 100 1253 28
304 info@brand.de
Containers
General Laboratory
Atomizers
PE-HD. By turning the nozzle, delivery
can be varied from a fine spray to a solid
stream. Pack of 5.
Capacity Cat. No.
ml
400 1441 80
850 1441 90
Spare stopcock
Fits all sizes. With PP adapter and 2 FKM
seals. Pack of 1
Cat. No. 1311 00
Storage bottles
Narrow mouth
PE-HD. Narrow mouth. With screw cap and carrying handle.
Pack of 1.
Capacity Ø Height Neck inner-Ø Cat. No.
l mm mm mm
www.brand.de 305
Containers
General Laboratory
Storage bottles
Wide mouth
PE-HD. With screw cap and carrying handle. Pack of 1.
Capacity Ø Height Neck inner-Ø Cat. No.
l mm mm mm
Jerry cans
PE-HD. With screw cap and threaded pourer.
Pack of 1.
Capacity Length Width Height Cat. No.
l mm mm mm
Buckets
PE-HD. With or without push-on lid. With
graduation and handle. Without spout.
Push-on lids for buckets PE-LD
Pack of 1.
(Please order push-on lid separately!) PE-LD. Pack of 1.
Capacity Height Cat. No. For bucket Cat. No.
l mm l
Buckets
PP. With spout, reinforced rim and handle.
Pack of 1.
Capacity Height Cat. No.
l mm
12 300 723 76
15 340 723 78
306 info@brand.de
Containers
General Laboratory
Trays (photographic trays)
PP, white. Ribbed bottom improves stability, reinforced rim, slightly sloping walls.
Stackable. Pack of 1.
Length Width Height Cat. No.
mm mm mm
Tidy tray
PVC. Designed for use as utility trays or drawer organizers. Ideal for storing magnetic
stirring bars, etc. Pack of 1.
Compartments Length Width Height Cat. No.
mm mm mm
Ice bucket
Durable, rigid polyurethane foam. For bench-top cold storage. Excellent insulating
properties. Sturdy, octagonal design for improved stability. Tapered side with molded
handles and flat lid for easy handling and storage. Suitable for chemical disinfection.
Operating temperature -196 °C to +95 °C. Pack of 1.
Capacity Length Width Height Cat. No.
l mm mm mm
www.brand.de 307
Pipette Rinsing Systems
Trouble-free and gentle cleaning of pipettes with repetitive siphon rinsing. Water supply
connection via hose nozzle, outer-Ø 12 mm. The complete cleaning system consists of a
rinsing apparatus, soaking jar and pipette basket. Please order each component separately.
Rinsing apparatus
PE-HD. Adjust the water flow to approx. 2 l/min. Pack of 1.
for pipette length Capacity inner-Ø Base Ø Height Cat. No.
mm l mm mm mm
Soaking jars
PE-HD. Pack of 1.
for pipette length Capacity inner-Ø Base Ø Height Cat. No.
mm l mm mm mm
Pipette baskets
PE-HD. Pack of 1.
for pipette length Basket height incl. handle Base Ø Basket height Cat. No.
mm mm mm mm
Pipette jar
with lid
PP, lid PE-HD. The jar is suitable for dust-proof storage of pipettes up to 400 mm long.
Pack of 1.
Base Ø Height inner-Ø Cat. No.
mm mm mm
308 info@brand.de
Draining Rack
Pipette Trays and Stand
General Laboratory
Draining rack
PS. Integrated wide draining trough. Pegs can be inserted as required. Peg holes are
closed at the back – preventing leakage toward the wall. Suitable for glass graduated
cylinders of up to 250 ml and beakers up to 1000 ml. Supplied complete with drain
tubing and accessories for wall mounting. Width 450 mm, height 630 mm, 72 pegs
(Ø 16 mm, length 120 mm). Pack of 1.
Cat. No. 951 05
Special pegs
for draining rack
PS. Special pegs for narrow-mouthed or small vessels, e.g., test tubes, small volumetric
flasks and graduated cylinders. Outer diameter 6 mm, length 120 mm. Pack of 11.
Cat. No. 951 11
Pipette stand
PP. Holds up to 94 pipettes of different
sizes. Rotary model, with additional rotary Pipette tray
tip rest to protect the pipette tips. Suitable
PVC. Practical tray for pipettes of any size.
for all graduated and one-mark pipettes.
Length 280 mm, width 220 mm, height
Slightly-curved stand base rim helps retain
29 mm. Pack of 1.
dripping liquids. Diameter 230 mm, height
450 mm. Pack of 1. Cat. No. 283 00
Pipette tray
PVC. Drawer organizer.
Four compartments for approx. 30 gradu-
ated pipettes (1 - 20 ml). Length 420 mm,
width 300 mm, height 30 mm.
Pack of 1.
Cat. No. 283 05
Pipette tray
PVC. Nine oblong dividers, open on one
side. For small-volume pipettes.
Length 355 mm, width 300 mm, height
45 mm. Pack of 1.
Cat. No. 309 00 Cat. No. 7685 15
www.brand.de 309
Water Stills
Water Stills
General Laboratory
Technical data
MonoDest MonoDest
3000 E 3000 N
310 info@brand.de
Cleaning
Cleaning
General Laboratory
Manual Detergents for Cleaning and Disinfection
Safe on tests
User-friendly
Detergents and disinfecting detergents can
be removed without residue. Thus, even Our cleaners and disinfecting cleaners have
sensitive tests in hematology, serology, a very broad spectrum of activity. Therefore,
microbiology and enzymatic analytical only a small number of products are needed
chemistry are not affected. Simply observe for a great variety of procedures.
the recommended concentrations and rinse These cleaners and disinfecting cleaners do
thoroughly with water. not contain caustic alkali. This is why they
are particularly gentle to material, and are
fully effective even in hard water.
Cost efficient
Normally soiled instruments will become
clean when using the recommended con-
centration of detergent. For heavy soiling,
it is recommended to heat the solution with-
in the indicated limits. However, an exces-
sively high concentration will not increase
the cleaning performance. Please note the
technical information on page 342-343.
Abbreviations:
Association for Applied Hygene (VAH)
Robert Koch-Institute (RKI)
German Society for Hygene and Microbiology (DGHM)
German Veterinary Medical Society (DVG)
www.brand.de 311
Detergents
Mucasol ®
General Laboratory
Universal detergent
Ingredients:
Liquid, alkaline high-performance concen- > 30% phosphates,
trate for immersion and ultrasonic baths. < 5% anionic surfactants, amphoteric
Universal cleaner for laboratory equipment surfactants, complexing agents, corrosion
and instruments made of glass, porcelain, inhibitors, auxiliary agents
plastic, rubber and metal. Concentration for use:
Items are cleaned in ~ 10 - 30 minutes, Ultrasonic bath:
without mechanical help. Mucasol® replaces 0.2 - 2% (1.4 - 14 ml/l)
chromosulfuric acid, is free from chlorine Immersion bath:
and caustic alkalis, is non-toxic, non- 0.7 - 3% (5 - 22 ml/l)
aggressive and biodegradable. Prolonged Chemical and physical specifications:
exposure of zinc and non-anodized Light green, clear liquid
aluminium surfaces should be avoided. Density: approx. 1.4 g/ml
pH value (0.7%): approx. 11.5
pH value (3%): approx. 11.7
Description Pack Cat. No.
of
EDISONITE ® CLASSIC
Universal detergent
Alkaline detergent. Powder compound for Ingredients:
immersion and ultrasonic bath. Ideal for re- > 30% phosphates,
moval of natural fats and oils, proteins and < 5% anionic surfactants, non-ionic surfac-
blood. Durable protection against corrosion tants, corrosion inhibitors, auxiliary agents
damage. Concentration for use:
0.5 - 1% (5 - 10 g/l)
Chemical and physical specifications:
Light green powder
Bulk density: approx. 850 kg/m3
pH value (0.5%): approx. 11.5
pH value (1%): approx. 11.5
Use on zinc and non-anodized aluminium is
restricted.
Description Pack Cat. No.
of
1 kg jar 6 443 01
5 kg bucket 1 442 05
EDISONITE ® SUPER
Universal detergent
Ingredients:
Neutral detergent. Powder compound for > 30% phosphates,
immersion and ultrasonic bath. Ideal for 5 - 15% anionic surfactants,
removal of natural fats and oils, proteins corrosion inhibitors, auxiliary agents
and blood. Concentration for use:
EDISONITE® SUPER is recommended for 0.5 - 1% (5 - 10 g/l)
optical glass, for plastics sensitive to Chemical and physical specifications:
alkalis and rubbers. Light green powder
Bulk density: approx. 800 kg/m3
pH value (0.5%ig): approx. 7.5
pH value (1%ig): approx. 7.5
Description Pack Cat. No.
of
1 kg jar 6 443 61
5 kg bucket 1 443 65
312 info@brand.de
Detergents
Mucocit ® -T
General Laboratory
Instrument disinfecting detergent Microbiological efficiency/
concentration for use:
Particularly suited for sensitive instruments.
Disinfection of instruments: Bacteria (incl.
Free of phosphates, aldehydes, phe-
Tuberculosis and mycobacteria) and fungi
nols and chlorine derivates. Fresh scent.
with high organic burden according to
CE-marked according to MPG (German
DGHM/VAH directives:
Medical Products Law).
1% (10 ml/l)/1 hour
2% (20 ml/l)/30 minutes
Ingredients:
3% (30 ml/l)/15 minutes
100 g Mucocit®-T contain:
Limited virucidal efficacy* (incl. HIV, HBV,
3.9 g didecyldimethylammoniumchloride,
HCV) 1% (10 ml/l)/5 minutes * As recommended by the RKI,
4.5 g alkylpropylenediamino- Bundesgesundheitsblatt 01/2004
Inactivation of rotaviruses:
1,5-bisguanidiniumacetate,
2% (20 ml/l)/5 minutes
2 g bis(-aminopropyl)-laurylamine,
2.8 g laurylpropylenediamine, Description Pack Cat. No.
5 - 15% non-ionic surfactants, of
auxiliary agents
Chemical and physical specifications: 2 liter bottle 6 448 22
Clear, blue liquid, fresh smell 5 liter can 3 448 25
Density: approx. 1 g/ml
pH value (1%, 20 °C): approx. 10.5
www.brand.de 313
Detergents
Pursept ® -A Xpress
General Laboratory
■ Effective in only 15 seconds Clear, colorless liquid, fresh smell Description Pack Cat. No.
■ DGHM/VAH-tested and Density: approx. 0.9 g/ml of
RKI-compliant pH value: approx. 7.5
Tissues: FCC-bleached (oxidation bleach), Dispenser box (150 tissues*) 6 448 40
■ Fresh scent
bio-degradable Refill unit (150 tissues*) 6 448 45
* 135 x 222 mm
Pursept® Wipes XL and Pursept®-A Xpress, to disinfect the workplace, lab table or clean
bench quickly and reliably. Wipes can be stored in the refillable dispenser box.
314 info@brand.de
General Laboratory
BRAND online
General Laboratory
Notes:
www.brand.de 315
© Robert Deutschmann/The Image Bank/Getty Images
Technical
Information
Quality Management..................................... p. 318
Certificates................................................. p. 320
Cleaning..................................................... p. 342
Quality Management
Technical Information
Declaration of conformity
For volumetric instruments to be ready for use in legally regulated
areas such as the medical and pharmaceutical fields (manufacture
and testing of medicinal products), the German Measurement and
Calibration Regulation requires a Declaration of Compliance from
the manufacturer. This is met with the mark "DE-M"*.
With the DE-M marking, the manufacturer declares that the
instrument in question meets the requirements of the German
Measurement and Calibration Regulation and applicable standards.
In general the product itself carries the DE-M marking or, with
disposables, the packaging.
* as of January 1, 2015
BRAND will change the mark on volumetric instruments to "DE-M" starting
January 1, 2015.
318 info@brand.de
Precision
Technical Information
Precision and Precision" mean in volumetric measuring?
➊ Error limit
The term "Error limit" (EL) in the corresponding standards
defines the maximum permissible deviation from the specified ➍ Partial volumes
value. Generally A and CV are related to the nominal volume (Vnominal ).
These values are in % and have to be converted for partial
➋ Accuracy (A) volumes (Vpart. ). In contrast, there is no conversion for partial
Accuracy (A) indicates the closeness of measured mean volume volumes, if A and CV are stated in volume units (e.g., ml).
to the specified value, i.e., systematic measurement variation.
Accuracy is defined
_ as the difference between the measured
mean volume ( V ) and the specified value (Vspec. ), related to the
➎ Error limit of A and CV
A good estimate for the error limit (EL) of the instrument, e.g.,
specified value in percent.
for the nominal volume ( Vnominal ), can be calculated using the
values for accuracy and coefficient of variation.
➌ Coefficient of Variation (CV)
Coefficient of variation (CV) indicates the closeness of values of
repeated measurements, i.e., random measurement variation. ➏ Precision (reproducilibity)
Coefficient of variation is defined as standard deviation in per- It describes the closeness in volume units between the different
cent, related to the mean volume. values in a set of measurements.
1 2 3 4 5
V - Vspec. s · 100 Vnominal A% + 2CV%
EL ≥ Vmeasured - Vspec. A [%] = · 100 CV [%] = A part. [%] = · A nominal% EL ≥ · Vnominal
Vspec. V Vpart. 100%
(analog CVpart. [%] )
www.brand.de 319
Certificates
Certificates
Technical Information
320 info@brand.de
Monitoring of
Measuring Instruments
Technical Information
GLP, ISO/IEC 17 025, ISO 9001 Testing
Analytical laboratories have to verify and The monitoring of measuring instruments The gravimetric test for liquid
document the accuracy of all measuring requires that the instrument's performance handling instruments is performed
instruments used in order to achieve reliable data be known and documented before according to DIN EN ISO 8655; for
analysis. This especially applies to laborato- being admitted to use and confirmed at ap- glass volumetric instruments, DIN
ries operating according to GLP guidelines, propriate intervals. The frequency of checks EN ISO 4787 is applied. Traceability
DIN EN ISO/IEC 17 025, or certified to depends on the results of previous calibra- of measuring devices to national
DIN EN ISO 9001. tions. standards needs to be ensured. For
These tests are necessary to ensure con- instruments calibrated to contain
tinued accuracy of instruments which may (TC, In) the mass of water con-
have changed due to aggressive chemicals, tained, and for instruments calibrat-
or method and frequency of cleaning. The ed to deliver (TD, Ex), the mass of
test cycle must be specified by the user. water delivered, will be weighed on a
Typical monitoring intervals for liquid hand- balance. The mass of water mea-
ling instruments are every 3-12 months; sured has to be adjusted to account
for glass volumetric instruments, every for parameters like water density
1-3 years. Performance certificates issued and air buoyancy on the balance.
Information for BRAND
by BRAND show all instrument data re- The thermal coefficient of expansion
Calibration Service on
quired for monitoring so initial inspection of glass volumetric instruments has
page 326.
may be eliminated. Also before disposing a to be taken into account.
measuring instrument, a final test is
neccessary (see DIN 32 937).
Testing scope
Performance certificates are supplied as
standard for liquid handling instruments While liquid handling instruments are
and for reusable BLAUBRAND® volumetric tested individually, the use of statis-
instruments (see pages 173, 320). tical methods to monitor glass volu-
metric instruments is recommended.
The following equation to determine
the minimum random quantity (a) out
of the total quantity (n) has proven
effective in actual use:
a = n
www.brand.de 321
Monitoring of
Measuring Instruments
Calculations
Technical Information
2. Calculation of accuracy
–
V - Vspec. 200.1514 - 200
A [%] = · 100 A [%] = · 100
Vspec. 200
A [%] = 0.076
322 info@brand.de
Calculations
Technical Information
3. Calculation of the standard deviation, necessary for the determination of coefficient of variation
0.8393
s = 1.0032 ·
9
s = 0.306
CV [%] = 0.153
The result for the calculated example is: What to do if the instrument is
Note:
not within the factory-specified
Results of the gravimetric testing: If there are no specifications for partial error limits?
volumes, the values A nominal [%] and
Tested volume (µl): 200.0000 1. Check whether all sections of the
CVnominal [%] which are related to the
Mean volume (µl): 200.1514 SOP are taken into account.
nominal volume Vnominal must be con-
A [%] 0.076
verted. 2. Follow the troubleshooting
CV [%] 0.153
guide in the operating manual for
A [%] specified* 0.600
For a partial volume of 20 µl this means: assistance.
CV [%] specified* 0.200
* Error limits specified by the manufacturer of instru-
Vnominal 3. Adjust the calibration of the
A 20 µl [%] = · A nominal [%]
ment. See your operating manual for specifications. V20 µl instrument in accordance with
the operating manual.
⇒ This pipette meets specifications. 200 µl
A 20 µl [%] = · 0.5% If despite these steps the instrument
20 µl still does not meet the specifications,
If the calculated values for Accuracy (A [%])
and Coefficient of Variation (CV [%]) are remove from service and contact
less than or equal to the factory published A 20 µl [%] = 5% BRAND for support.
specifications, the instrument is calibrated
to operate within specifications. The calculation of CV20 µl is analog.
www.brand.de 323
Easy Calibration Technique
ISO 9001 and GLP guidelines require routine calibration (approx. ■ No need to send the instruments out
every 3-12 months) and readjustment of measuring instruments for calibration and adjustment.
if necessary. Typically time-consuming readjustment can be
■ Accuracy can be adjusted to meet the
finished in seconds with BRAND Liquid Handling instruments.
needs of your special applications.
■ No tools necessary for adjustment.
The following BRAND Liquid Handling instruments are equipped Adjustments can be performed in
with this time-saving adjustment technology: seconds.
Dispensette® Easy Calibration
Transferpette®-8/-12 electronic
-8/-12
Transferpette® electronic
Transferpette®-8/12
Transferpette®
Transferpette®
Transferpette®
Titrette®
Easy Calibration of
mechanical instruments
(e.g., bottle-top dispenser Dispensette®)
Example:
Gravimetric testing yields a delivered volume of 9.90 ml with
a set volume of 10 ml (e.g., after a longer period of usage
or for specific applications). Adjustment is quick and easy in
five steps:
1. Open housing by sliding the 2. Lift gear lock lever to
latch and removing the front. release.
3. Pull the red knob and 4. Reposition red knob and 5. Replace housing – done!
set the display to actual gear lock lever to the Alteration of factory setting
delivered volume (e.g., original position. is indicated by a red re-
9.90 ml). calibration flag.
324 info@brand.de
Easy Calibration Technique
Technical Information
Easy Calibration of 1. Initial display
electronic instruments
(e.g., Transferpette® electronic
microliter pipette)
EASYCAL ™ 4.0
To further facilitate calibration, BRAND has developed EASYCAL™
calibration software. Instrument specific Standard Operating Proce-
dures (SOPs) describe step-by-step testing procedures. All neces- BRAND also offers a factory cali-
sary calculations are carried out automatically by the user-friendly bration service. Please see page
software (please see page 109 for more information). A free demo 326 for more information.
version of EASYCAL™ and SOPs are available for download from
www.brand.de.
www.brand.de 325
Calibration Service
Instruments used for control, inspec- BRAND offers full calibration service includ-
tion, measuring and testing require ing instrument adjustment and repair. This
written protocols for routine testing reduces service downtime, saves money
and calibration. A testing schedule and provides an independent review organi-
and inspection and testing proce- zation for the calibration of the instruments.
dure must be defined. Documenta-
tion is required of the Accuracy and BRAND calibration service is available for:
Coefficient of Variation testing that
■ Piston-operated pipettes
is performed.
(single and multichannel)
Frequent confirmation can become
time consuming and expensive. ■ Bottle-top dispensers
Measuring instruments must be ■ Digital bottle-top burettes
taken out of service and may have ■ Repetitive pipettes (stepper)
to be adjusted or repaired. Maintain-
ing an in-house calibration lab with
specially trained personnel can be
very costly.
326 info@brand.de
Calibration Certificates
Technical Information
Works calibration certificate DAkkS calibration certificate
Issued by BRAND works calibration service. Issued by the DAkkS calibration laboratory at BRAND
(also available for volumetric instruments of glass and pla-
stics). You will find more information on the following pages.
www.brand.de 327
Monitoring of
Measuring Instruments
Since the beginning of 2010, DAkkS (Deutsche Akkreditierungsstelle GmbH) has been the
sole provider of accreditation services for regulatory matters in Germany. The legal founda-
tions for DAkkS are Regulation (EC) No. 765/2008 and the German Federal Accreditation
Law (AkkStelleG).
BRAND calibrates the following volumetric instruments, no matter if they are new or already
in use:
A major difference between works calibration services and DAkkS laboratories is the accu-
rate determination of the respective uncertainty of measurement guaranteed by the accred-
ited laboratory and supervised by the DAkkS.
328 info@brand.de
Monitoring of
Measuring Instruments
Technical Information
DAkkS – A member in the International Accreditation Network
DAkkS is a member of the International Laboratory Accreditation Cooperation (ILAC),
the highest level international institution for laboratory calibration, and is a signatory to the
MRA – Mutual Recognition Agreements.
BIPM
International Bureau of Weights and Measures
PTB
National metrology institute National Calibration laboratory
(e.g. PTB in Germany) Standard for volumetric instruments,
accredited to DIN EN
ISO/IEC 17 025
BRAND calibration service DAkkS DAkkS calibration
Calibration Service Eichamt* for volumetric instruments, certificate
(e.g. DakkS at BRAND) certified according to
DIN EN ISO 9001
BRAND
Works certificate:
User individual or
batch certificate,
USP certificate
Measuring Instruments
Products
Traceability to international standard
The BIPM (Bureau international des poids et measures) ensures the world-
wide uniformity of measurements and weights.
Depending on the legal framework, there are two pathways to trace- Traceability to national standard
ability, which both lead to the BIPM (International Bureau of Weights and
Measures): Calibration, or – for all measuring instruments that fall under the
In Germany, the PTB (Physikalisch-Technische Bundesanstalt) is the national
Verification Act – verification.
metrology institute. It observes the traceability to national standard.
* German State Office of Weights and Measures
www.brand.de 329
CE Marking
Technical Information
IVD Directive
330 info@brand.de
Materials: Glass
Laboratory Glass
Technical Information
General Properties
Glass has very good chemical resistance against water, saline solutions, acids, alkalis and
organic solvents and in this respect surpasses the majority of plastics. It is only attacked
by hydrofluoric acid, and – at elevated temperatures – by strong alkalis and concentrated
phosphoric acid. Further advantages of glass are its dimensional stability, even at elevated
temperatures, and its high transparency.
www.brand.de 331
Materials: Glass
Chemical Resistance
Technical Information
Chemical interaction of glass with water and acids Chemical interaction of glass with alkalis
The chemical interaction of water and acids with glass surfaces Alkalis attack glass surfaces as concentration and tempera-
is negligibly small; only very small amounts, primarily monovalent tures increase. Borosilicate glass 3.3 (Boro 3.3) limits surface
ions, are dissolved from the glass. This forms a very thin, almost erosion to the µm range; however, after prolonged exposure,
non-porous layer of silica gel on the glass surface, inhibiting fur- volume changes and/or graduation destruction may occur.
ther attack. Exceptions are hydrofluoric acid and hot phosphoric
acid which prevent the formation of the inert layer.
0.03 8
c(NaOH) = 1 mol/l
HCl time: 1 Std.
temperature: 100°C
0.02
Temperature:
100 °C
layer attack (µm)
0.02 0.01
0.01
0 3 6 0 4 8 12 0 3 6
time (h) normality of the acids time (h)
Hydrolytic attack on Boro 3.3 Acid attack on Boro 3.3 Alkali attack on Boro 3.3
as a function of time as a function of acid concentration as a function of time
Hydrolytic resistance of
glass grains Acid resistance Alkali resistance
Borosilicate glass 3.3 meets hydro- Borosilicate glass 3.3 meets class Borosilicate glass 3.3 meets class
lytic resistance class 1 of DIN ISO 1 of DIN 12 116, which is divided 2 of DIN ISO 695, which is di-
719 (98 °C), which is divided into 5 into 4 acid resistance classes. vided in 3 alkali resistance classes.
hydrolytical resistance classes. This Borosilicate glass is also called Surface erosion after 3 hours of
means that when glass grain with acid-resistant borosilicate glass, as boiling in a mixture with equal vol-
a granulation rate of 300-500 µm the surface erosion after 6 hours umes of sodium hydroxide solution
is exposed to water at 98 °C for of boiling in 6 N HCl is less than (1 mol/l) and sodium carbonate
1 hour, less than 31 µg Na2O per 0.7 mg/100 cm2. Removal of alkali solution (0.5 mol/l) is approximately
gram of glass grain will be removed. oxide according to DIN ISO 1776 is 134 mg/100 cm2.
less than 100 µg Na2O/100 cm2.
In addition, Borosilicate glass 3.3
also meets class 1 of DIN ISO 720
(121 °C), which is divided into 3
hydrolytical resistance classes. This
means that when glass grain is ex-
posed to water at 121 °C for 1 hour,
less than 62 µg Na2O per gram of
glass grain will be removed.
Chemical resistance to Water DIN ISO 719 Acids DIN 12 116 Alkalis DIN ISO 695
(HGB Class 1-5) (Class 1-4) (Class 1-3)
332 info@brand.de
Materials: Glass
Mechanical Resistance
Technical Information
Thermal stresses Resistance to temperature changes Mechanical stresses
During the production and processing of When glass is heated to a temperature From a technical viewpoint, glasses
glass, hazardous thermal stresses may be below the lower annealing point, thermal behave in an ideally elastic way.
introduced. During the cooling of molten expansion and the poor thermal conductiv- This means that, after exceeding
glass, the transition from the plastic state ity result in tensile and compressive stress. the limits of elasticity, tensile and
to the brittle state takes place in the range If, due to improper heating or cooling rates, compressive stress does not result
between the upper and lower annealing the permissible mechanical strength is in plastic deformation, but break-
points. At this stage, existing thermal stress exceeded, breakage occurs. Apart from age occurs. The tensile strength is
must be eliminated through a carefully con- the coefficient of expansion α, which varies relatively low and may be further di-
trolled annealing process. Once the lower with each kind of glass, the wall thickness, minished by scratches or cracks. For
annealing point is reached, the glass may the geometry of the glass body, and any ex- safety reasons, the tensile strength
be cooled more rapidly, without introducing isting scratches must be taken into account. of Boro 3.3 in apparatus and plant
any major new stress. Therefore, it is difficult to state specific nu- design is calculated at 6 N/mm2.
Glass responds in a similar way when merical values for thermal shock resistance. The compressive strength, however,
heated, e.g., through direct exposure to However, a comparison of the α values is approximately ten times as high.
a Bunsen flame, to a temperature higher shows that Boro 3.3 is much more resistant
than the lower annealing point. Uncontrolled to thermal changes than, e.g., AR-GLAS®.
cooling may result in the "freezing in" of
thermal stress which would considerably
reduce resistance to breakage and me-
chanical stability.
To eliminate inherent stress, glass must be
heated up to a temperature between the
upper and lower annealing point, be kept
at this temperature for approx. 30 minutes
and be cooled by observing the prescribed
cooling rates.
20
12
10
8 Softening point
Working range
p.e. sintering, pressing,
6
drawing, blowing,
log η in dPas
melting, casting
4
Temperature in °C
Upper annealing point Lower annealing point Linear expansion coefficient Density
(viscosity 1013 dPas) (viscosity 1014.5 dPas) α 20/300 10-6 K-1 g/cm3
www.brand.de 333
Materials: Plastic
n Elastomers n Thermoplastics
Polymers with loosely cross-linked mol- Polymers with a linear molecular structure
ecules, exhibiting rubber-like elasticity with or without side branches are trans-
at room temperature. Heating causes formed into objects during molding opera-
irreversible curing (vulcanization). tions without changing their thermoplastic
The most popular elastomers are properties. Thermoplastics are the materials
natural rubber and silicone rubber. commonly used in plastic labware produc-
tion. Hence we provide here a brief descrip-
tion of some individual plastics explaining
their structural, mechanical, chemical and
n Thermosets
physical properties. The most popular ther-
Polymers with tightly cross-linked molecules moplastics are polyolefins like polyethylene
are very hard and brittle at room tempera- and polypropylene.
ture; heating causes irreversible curing.
These plastics are rarely used for plastic
labware. The best known thermosets are
the melamine resins. Melamine resin is
produced by polycondensation of melamine
with formaldehyde.
PS Polystyrene
Polystyrene is glass-clear, hard, brittle, and
dimensionally stable due to its amorphous
structure. PS has good chemical resistance
to aqueous solutions but limited resistance
to solvents. Disadvantages include low ther-
PC Polycarbonate
mal stability and its tendency to suffer from These are thermoplastic linear carboxylic
stress-cracks. acid polyesters combining many of the
properties of metals, glass and plastics.
The materials are transparent and have
good thermal properties between -130 to
SAN Styrene-acrylonitrile copolymer +130 °C. Note: PC may be weakened by
autoclaving or exposure to alkaline deter-
This is a glass-clear material with good gents.
resistance to stress-cracking. It has slightly
better chemical resistance than PS.
PA Polyamide
PMMA Polymethyl methacrylate Polyamides are linear polymers with re-
peating amide chain linkages. With their
Rigid, glass-clear ("organic glass"). Resis- favorable strength characteristics and high
tant to atmospheric agents. Replaces glass durability, polyamides can often be used as
in many applications where temperatures structural materials and for surface coating
are below 90 °C and low chemical resis- metals. They have good chemical resistance
tance is required. PMMA has excellent UV against organic solvents, but are easily at-
radiation stability. tacked by acids and oxidizing agents.
334 info@brand.de
Materials: Plastic
Technical Information
Ethylene-Tetrafluoroethylene
The vinyl chloride polymers are mainly The polymerization of ethylene under
copolymer
amorphous thermoplastics with very good high-pressure results in a certain number
chemical resistance. of branches in the chain. The result is a ETFE is a copolymer of ethylene with
Their combination with plasticizers opens less compact molecular structure than chlorotrifluoroethylene and/or with tetra-
up many useful applications, ranging PE-HD, with very good flexibility and fluoroethylene. This plastic is remarkable
from artificial leather to injection molding good chemical resistance, but less chemi- for its excellent chemical resistance, but
components. PVC has good chemical cal resistance to organic solvents than its temperature stability is lower in com-
resistance, especially with oils. PE-HD. Use is limited to temperatures parison with PTFE (at most 150 °C).
below 80 °C.
www.brand.de 335
Materials: Plastic
General Properties
Technical Information
Resistance to breakage and low A variety of parameters should be consid- The recommendations listed below are based
weight are important advantages of ered: exposure time and concentration of on technical literature and information pro-
plastics. The application determines chemicals, thermal stress (e.g., autoclaving), vided by the manufacturers of raw materi-
which plastic to select. exertion of force, exposure to UV radiation, als. They were prepared carefully and are
and aging, which may be caused by the ac- intended as general guidance. However, they
tion of detergents, or other environmental cannot replace suitability testing perfomed
factors. by the user under actual working conditions.
Physical Properties
Max. operating Brittle Micro wave Density Elasticity Transparency
temperature temperature suitability* (g/cm3)
(°C) (°C)
Sterilization
Autoclaving* b / γ - radiation Gas Chemical
at 121 °C (2 bar), 25 kGy (ethylene oxide) (formalin,
acc. DIN EN 285 ethanol)
PS no yes no yes
SAN no no yes yes
PMMA no yes no yes
PC yes1) yes yes yes
PVC no2) no yes yes
POM yes1) yes (restricted) yes yes
PE-LD no yes yes yes
PE-HD no yes yes yes
PP yes yes (restricted) yes yes
PMP yes yes yes yes
ETFE yes no yes yes
PTFE yes no yes yes
FEP/PFA yes no yes yes
PUR yes 3) – yes yes
FKM yes – yes yes
EPDM yes – yes yes
NR no no yes yes
SI yes no yes yes
* Before autoclaving, labware must be carefully cleaned and rinsed with distilled water. Always remove covers from containers!
1)
Frequent autoclaving reduces mechanical stability.
2)
With the exception of PVC tubing, which is autoclavable up to 121 °C.
3)
Frequent autoclaving reduces elasticity.
336 info@brand.de
Materials: Plastic
Technical Information
Biological Properties
The following plastics are generally non-toxic to cell cultures:
PS, PC, PE-LD, PE-HD, PP, PMP, PTFE, FEP, PFA.
Chemical Properties
With regard to chemical resistance, plastics are classified as follows:
+ o –
Excellent chemical resistance Good to limited chemical resistance Poor chemical resistance
Continuous exposure to the substance Continuous exposure to the substance Not suitable for continuous expo-
does not cause damage within 30 days. causes minor damage, some of which is sure to the substance. Immedi-
The plastic may remain resistant for reversible, within 7-30 days (e.g., swell- ate(!) damage may occur (loss
years. ing, softening, decrease of mechanical of mechanical strength, defor-
strength, discoloration). mation, discoloration, cracking,
dissolution).
PS Polystyrene
SAN Styrene-acrylonitrile copolymer
PMMA Polymethyl methacrylate
PC Polycarbonate
PVC Polyvinyl chloride
POM Polyoxymethylene
PE-LD Low-density polyethylene
PE-HD High-density polyethylene
PP Polypropylene
PMP Polymethylpentene
ETFE Ethylene-tetrafluoroethylene copolymer
PTFE Polytetrafluoroethylene
FEP Perfluoroethylene-propylene copolymer
PFA Perfluoroalkoxy copolymer
PUR: Polyurethane
FKM Fluoro elastomer
EPDM Ethylene-propylene-diene-rubber
NR Natural rubber
SI Silicone rubber
www.brand.de 337
Materials: Plastic
Chemical Resistance
Technical Information
338 info@brand.de
Materials: Plastic
Technical Information
The data for the chemical resistance of salts also apply to their aqueous solutions.
PP PMP ETFE PTFE FEP/PFA FKM EPDM NR SI
20 °C 50 °C 20 °C 50 °C 20 °C 50 °C 20 °C 50 °C 20 °C 50 °C 20 °C 20 °C 20 °C 20 °C
Acetaldehyde + – o – + o + + + + – o – –
Acetic acid (glacial) 100% + o + o + + + + + + – o o o
Acetic acid 50% + + + + + + + + + + – – – –
Acetic anhydride o o + o + + + + + + – o o o
Acetone + + + + + o + + + + – + o –
Acetonitrile + o o – + + + + + + – – – –
Acetophenone o o o – + + + + + + – + – –
Acetyl chloride + + + + + + + + – – –
Acetylacetone + + + + + + + + – + – –
Acrylic acid + + + + + + + + – – – –
Acrylonitrile o – – – + + + + + + – – – –
Adipic acid + + + + + + + + + + + + + +
Allyl alcohol (2-Propene-1-ol) + + + o + + + + + + + + o –
Aluminium chloride + + + + + + + + + + + + o o
Aluminium hydroxide + + + o + + + + + + + + + +
Amino acids + + + + + + + + + + + + + +
Ammonium chloride + + + + + + + + + + + + + +
Ammonium fluoride + + + + + + + + + + o + – +
Ammonium hydroxide 30% (Ammonia) + + + + + + + + + + – + + o
Ammonium sulfate + + + + + + – + o o
n-Amyl acetate o – + o + + + + + + – o o –
n-Amyl alcohol (Pentanol) + + + + + + + + + + o o o –
Amyl chloride (Chloropentane) – – – – + + + + + + + – – –
Aniline + + + o + o + + + + – – – –
Aqua regia – – – – + + + + + + – – – –
Barium chloride + + + + + + + + + + + + + +
Benzaldehyde + + + + + o + + + o – o – –
Benzene + o o o + + + + + + o – – –
Benzine (gasoline) o o o o + + + + + + + – – –
Benzoyl chloride + o o o + + + + + – – –
Benzyl alcohol o – o – + + + + + + + o – o
Benzylamine o o + + + + + + + o – o
Benzylchloride + + + + + – – –
Boric acid, 10% + + + + + + + + + + + + + +
Bromine – – – – + + + + + + o – – –
Bromobenzene – – – – o – + + + + + – – –
Bromoform – – – – + + + + + + – –
Bromonaphthalene + + + + + +
Butanediol + + + + + + + + + + – + o –
1-Butanol (Butyl alcohol) + + + o + + + + + + + o + o
n-Butyl acetate o o + o + + + + + + – o – –
Butyl methyl ether + o + – + o + + + + – – – –
Butylamine + + + + – – – o
Butyric acid – – + + + + + + o – – –
Calcium carbonate + + + + + + + + + + + + + +
Calcium chloride + + + + + + + + + + + + + +
Calcium hydroxide + + + + + + + + + + + + + o
Calcium hypochlorite + + + o + + + + + + + + – o
Carbon disulfide – – – – + o + + + + + – – –
Carbon tetrachloride – – – – + + + + + + + – – –
Chloro naphthalene + + + + + – – –
Chloroacetaldehyde + + + +
Chloroacetic acid + o + o + + + + + + o o – –
Chloroacetone + + + + – + o –
Chlorobenzene – – – – + o + + + + o – – –
Chlorobutane o – o – + + + + + + o – – –
Chloroform – – o – + o + + + o o – – –
Chlorosulfonic acid o – + + + + – – – –
Chromic acid 10% + + + + + + + + + + + – – o
Chromic acid 50% o o o o + + + + + + + – – –
Chromosulfuric acid – – o – + + + + + + + – – –
Copper sulfate + + + + + + + + + + + + o +
Cresol o o – – + o + + + + + – – –
Cumene (Isopropyl benzene) o – – – + + + + + + + – – –
Cyclohexane o – – – + o + + + + + – – –
Cyclohexanone o – o o + + + + + + – – – –
Cyclopentane o – o – + + + + + + + – – –
Decane o o + + + + + + + – – o
Decanol + + + + + + + + + + o o
Dibenzyl ether + o + + + + + + – o – –
Dibromoethane o + + +
Dibutyl phthalate + o + o + + + + + + o o – o
Dichlorbenzene o – – – + o + + + + + – – –
Dichlormethane (Methylene chloride) o – o – o o + + + + o – – –
Dichloroacetic acid o – + + + o + + + + – – – –
Dichloroethane o – o – + + + + + + o – – –
Diesel oil (Heating oil) + o o – + + + + + + + – – –
Diethanolamine o + + o
Diethyl ether o – – – + + + + + + – – – –
Diethylamine o – o o + o + + + + – o o
Diethylbenzene – – – – + o + + + + + – – –
Diethylene glycol + + + + + + + + + + + + + o
Dimethyl sulfoxide (DMSO) + + + + + + + + + + +
Dimethylaniline + + + + + + o o – o
Dimethylformamide (DMF) + + + + + + + + + + – o o o
1.4 Dioxane + o o o + o + + + + – o – –
Diphenyl ether + + o – – –
Ethanol (Ethyl alcohol) + + + o + + + + + + o + o o
Ethanolamine + + + + + + + – + – –
Ethyl acetate + o o – + + + + + + – o –
Ethyl methyl ketone + o – – o o + + + + – o –
Ethylbenzene – – – – o o + + + + o – – –
Ethylene chloride o – – – + + + + + + o – – –
www.brand.de 339
Materials: Plastic
340 info@brand.de
Materials: Plastic
The data for the chemical resistance of salts also apply to their aqueous solutions.
Technical Information
PP PMP ETFE PTFE FEP/PFA FKM EPDM NR SI
20 °C 50 °C 20 °C 50 °C 20 °C 50 °C 20 °C 50 °C 20 °C 50 °C 20 °C 20 °C 20 °C 20 °C
Ethylene glycol (Glycol) + + + + + + + + + + 0 + 0 +
Ethylene oxide o – o – + + + + + + – – – –
Fluoroacetic acid + – – – –
Formaldehyde 40% + + + + + + + + + + o + o o
Formamide + + + + + + + + + + o o +
Formic acid 98-100% + + + o + + + + + + – o o –
Glycerol + + + + + + + + + + o + o +
Glycolic acid 70% + + + + + + + + + + o + + +
Heating oil (Diesel oil) + o o – + + + + + + + – – –
Heptane o o o o + + + + + + + – – o
Hexane + o o – + + + + + + + – – o
Hexanoic acid + +
Hexanol + + + + + + + + + + + – o o
Hydriodic acid + + + + + + + + + + + +
Hydrobromic acid + + + + + + + + + + + o o –
Hydrochloric acid 10% + + + + + + + + + + + + o o
Hydrochloric acid 20% + + + + + + + + + + + + o –
Hydrochloric acid 37% + + + + + + + + + + o + o –
Hydrofluoric acid 40% + + + + + + + + + + o o – –
Hydrofluoric acid 70% + o + o + + + o + + – – – –
Hydrogen peroxide 35% + + + + + + + + + + + o – o
Iodine-potassium iodide solution + + + o + + + + + + + + + –
Isoamyl alcohol + + + + + + + + + + o o o o
Isobutanol (Isobutyl alcohol) + + + + + + + + + + + + + +
Isooctane + + + + + + + – – –
Isopropanol (2-Propanol) + + + + + + + + + + + + + o
Isopropyl ether – – – – + o + + + + – – – –
Lactic acid + + + + + + + + + + + o o o
Mercury + + + + + + + + + + + + + +
Mercury chloride + + + + + + + + + + + + + +
Methanol + + + + + + + + + + – + o +
Methoxybenzene + + + + + + – – – –
Methyl butyl ether + + + o + o + + + + – – – –
Methyl formate (Methyl methanoate) + + + + + + o – o
Methyl propyl ketone + o o o + + + + + + – o – –
Methylene chloride (Dichloro methane) o – – – + + + + + + o – – –
Mineral oil (Engine oil) + + + + + + + + + + + – – o
Nitric acid 10% + + + + + + + + + + o o – –
Nitric acid 30% o – o – + + + + + + o – – –
Nitric acid 70% – – – – + + + + + + – – – –
Nitrobenzene – – – – + + + + + + – – – –
Oleic acid + + + + + + o – – –
Oxalic acid + + + + + + + + + + + + o o
Ozone o – + + + + + + + + + + – +
n-Pentane + + + + + + + – – –
Peracetic acid + + + + + + –
Perchloric acid + – o – + + + + + o + o – –
Perchloroethylene – – – – + + + + + + o – – –
Petroleum o – o o + + + + + + + – – o
Petroleum ether + + + + + + + – – –
Phenol + + o o + + + + + + o – – –
Phenylethanol o + + + + + +
Phenylhydrazine o + + + + + + o – o –
Phosphoric acid 85% + + + + + + + + + + + o – –
Piperidine + + + + + + + – – – –
Potassium chloride + + + + + + + + + + + + + +
Potassium dichromate + + o + o o
Potassium hydroxide + + + + + + + + + + – + o –
Potassium permanganate + + + + + + + + + + + + o –
Propanediol (Propylene glycol) + + + + + + + + + + + + + +
Propanol + + + + + + + + + + + + + o
Propionic acid + o + o + o + + + + + o – –
Pyridine o o + o – – + + + + – – – –
Salicylaldehyde + + + + + – + + + +
Salicylic acid + + + + + + + + + + + + + +
Silver acetate + + + + + + + + + + + + + +
Silver nitrate + + + + + + + + + + + + + +
Sodium acetate + + + + + + + + + + – + + o
Sodium chloride + + + + + + + + + + + + + +
Sodium dichromate + + + + + + + + + + + + + o
Sodium fluoride + + + + + + + + + + + + o o
Sodium hydroxide + + + + + + + + + + o + o o
Sulfuric acid 60% + + + + + + + + + + + – – –
Sulfuric acid 98% – – + + + + + + + + + – – –
Tartaric acid + + + + + + + + + + + o + +
Tetrachloroethylene o + + + o – – –
Tetrahydrofuran (THF) o – o – + o + + o o – – – –
Tetramethylammonium hydroxide + + + + + + – +
Toluene o – o – + + + + + + o – – –
Trichloroacetic acid o – + + + o + + + + – o o o
Trichlorobenzene – – o o + o + + + +
Trichloroethane – – – – + + + + + + + – – –
Trichloroethylene – – – – + + + + + + o – – –
Trichlorotrifluoro ethane o – + + + +
Triethanolamine + + + + – o o –
Triethylene glycol + + + + + + + + + + + + o +
Trifluoro ethane + + + o + – – –
Trifluoroacetic acid (TFA) + o + – –
Tripropylene glycol + + + + + + + + + + + +
Turpentine – – o o + + + + + + + – – –
Urea + + + + + + + + + + + + + +
Xylene – – o – + + + + + + o – – –
Zinc chloride + + + + + + + + + + + + + +
Zinc sulfate + + + + + + + + + + + + o +
www.brand.de 341
Cleaning (Basics)
Cleaning
Technical Information
342 info@brand.de
Cleaning (Basics)
Gentle Cleaning
Technical Information
For gentle treatment of labware, clean immediately after use – at low temperatures,
with brief soaking times, and at low alkalinity. Glass volumetric instruments should not be
exposed to prolonged immersion times in alkaline media above 70 °C, as such treatment
might cause volume changes through glass corrosion, and destruction of graduations.
Information .
50
Typical curve of alkali attack on Boro 3.3 in Typical curve of alkali attack on Boro 3.3 in
relation to temperature, calculated from weight relation to pH value, at 100 °C.
loss. c (NaOH) = 1 mol/l. Exposure time: 3 hours.
Exposure time: 1 hour.
www.brand.de 343
Safety Information
Safety Information
Technical Information
■ Before use, laboratory instru- ■ Instruments to be repaired must be ■ Waste must be disposed of according to
ments must be examined by the cleansed of all residues and be sterilized, local laws and regulations. This applies
user for suitability and functional- as necessary. Radioactively contami- also to disposable articles. It must not
ity. nated items must be decontaminated as pose a hazard to human beings or the
prescribed by the radiation protection environment.
■ Before use, laboratory instru-
authorities. Volumetric glass instruments
ments should be examined for ■ Please properly dispose of laboratory
(e.g., volumetric flasks, graduated cylin-
potential damage, especially glassware, being sure to remove any
ders, etc.) should not be repaired when
instruments subjected to pressure potential contaminants. Please note that
damaged. Exposure to heat may result in
or vacuum (e.g., desiccators, laboratory glassware is not recyclable.
residual stress within the glass (greatly
filter flasks, etc.).
increasing the probability of breakage),
■ The hazards of working with or an uncontrolled cooling process may
defective labware should never lead to permanent volume alterations.
be under-estimated (e.g., cuts,
burns, risk of infection). If a pro- Cutting down damaged graduated cylin-
fessional repair is not practical, ders shortens the distance between the
properly dispose of such items. upper graduation mark and the spout, as
defined by DIN, resulting in an increased
■ Always hold pipettes near the
danger of chemicals being spilled.
suction end, and carefully insert
the pipette into the adapter of
the pipette controller until it is
securely and firmly seated. Do
not use force. Broken glass can
cause injury!
344 info@brand.de
Index
General Terms and Conditions
C
Alphabetical Index
Number Index
B
General Terms and
Conditions
3 2 A
www.brand.de 345
Index Alphabetical Index
346 info@brand.de
Alphabetical Index
Index
Disposables Fuchs-Rosenthal counting chambers 256 Individual certificate BLAUBRAND®,
filter tips 76, 77, 80, 81 Funnel 272-275 information 320
ground joint sleeves 288 Buchner 274 Inoculation loops, disposable 244
inoculation loops 244 carboy 273 Insert System 159-162
micro haematocrit capillaries 252 filter 276 microplates 160, 162
micro tubes 119-124 holders 275 strips 160-162
micropipettes, intraMARK powder 273 intraEND, micropipettes 251
and intraEND 251 separating 275 intraMARK, micropipettes 251
microplates 143-163 standard ground joint 274 IVD Directive, Information 330
pasteur pipettes 249, 250
PCR plates 130-132 G J
PCR tubes 126-129 Gas Jars 298
PD-Tips 95, 96 sampling tubes 281 Jerrycans 306
Petri dishes 244 washbottles 280, 281
pipette tips Gay-Lussac L
(bulk packed, racked) 74-77 Alcoholometer 227 Laboratory
sample cups for analyzers 240, 241 Pyknometer 219 bottles 299
sample tubes 247 Glass beakers 265 spatulas 296
sedimentation tubes 247 Glass (information) 331-333 tubing 283
stirring spatulas 167 Graduated Ladles, sampling dippers 268
stoppers 246, 247 beakers 266, 267 Leak testing unit,
urine beaker 242 beakers with handle 267 for Transferpette® 105-108
UV Cuvettes 166 cylinders, glass 190-193 Life Science products
Distillation cylinders, plastic 194, 195 (information) 115-118
apparatus (water) 310 cylinders, USP 191 Low-temperature thermometers 233
thermometers (ground joint) 236 pipettes 177-182
Draining rack 309 pipettes, piston type 182 M
Drechsel gas washbottles 280 pipettes, plastic 182 macro, pipette controller 100
Dropping pipettes, USP 178 Magnetic stirring bar retriever 295
bottles 304 Grip stopper, for sample tubes 247 Magnetic stirring bars 292-295
pipettes, disposable 249 Ground joint spherical 295
point thermometer 234 bottles 193, 212, 299, 300 Malassez, counting chambers 256
Drying tubes 27 clips 288 Mat cover
funnels 274 for deep well plates 139
grease (silicone paste) 288 for PCR-tubes 133
E sleeves, disposable 288 Materials, glass (information) 331-333
Easy Calibration Technique 324, 325 socket with grip collar, PTFE 289 Materials, plastics (information) 334-341
EASYCAL™ 4.0 stopcocks, for burettes 214 Maximum thermometers 232
Calibration software 109-112 stoppers 193, 212, 290 Membrane filters for
EDISONITE® 312 thermometers 236 pipette controllers 99, 100
EDISONITE®-SUPER 312 Micro burettes, Bang pattern, 198
Electronic pipettes H micro-classic, pipette controller 101
multichannel 65-67 Haemacytometer 253-256 Micro haematocrit capillaries 252
single channel 63, 64 Haemacytometer cover glasses 257 micro pipette controller 101
Enclosed-scale Haematocrit sealing compound 252 Microcentrifuge tube
thermometers 230, 232, 236 HandyStep® electronic 91-94 cap inserts 124
Erlenmeyer flasks 265 HandyStep® S 87-90 disposable 116-124
Error limits, accuracy and coefficient Hellendahl staining trough 258, 259 rack 125, 142, 248
of variation (information) 319 High-temperature thermometers 233 screw cap 124
Evaporating dishes 269, 270 Holders Microliter pipettes
for funnels 275 Transferpette® S 49, 50
F for intraEND and Delbrück Transferpette® S -8/-12 51, 52
Faeces container 242 micropipettes 252 Transferpette® 55, 56
Fastening clip for oxygen flask 219 for separating funnels 275 Transferpette® -8/-12 58, 59
Film, PARAFILM® M Sealing Film 264 Hydrometers 224-228 Transferpette® electronic 63, 64
Filter alcoholometer 227 Transferpette® -8/-12 electronic 65-67
adapters 276 Baumé 226 Transferpettor 83-86
crucibles 276 Brix 227 Micropipettes 251
flasks (Buchner filter flasks) 277 cylinders 228 Microplates/
funnels 276 general purpose 225-227 BRANDplates® 137,138, 143-163
funnel supports 275 range finder 225 96-well 137, 146, 152, 157
tips 76, 77, 80, 81 special-purpose 228 384-well 147, 153, 158
tips, low retention 80, 81 sugar hydrometers, 1536-well 148, 158
Flasks Saccharometers 227 lids 164
Erlenmeyer 265 strip plates 146, 152
volumetric 184-189 I for Insert System 160, 162
Kohlrausch 188 Ice bucket 307 Mini cooler 125, 142
plastic 189 Imhoff for PCR 132
trapezoidal 184 cone rack 221, 222 for tubes 125, 142
Flow indicator 283 sedimentation cone 223 Mixing cylinders 193
Forceps 297
www.brand.de 347
Index Alphabetical Index
348 info@brand.de
Alphabetical Index
Index
Sealing film Supports U
for plates (Life Science) 140 for Transferpette® electronic 64 Universal detergents 312
PARAFILM® M 264 for Transferpettor 86 Urine beaker 242
Sedimentation cones, Imhoff 221, 222 Surface disinfecting detergents 313, 314 USP
Sedimentation bulb pipettes 175
cone racks 223 T graduated cylinders 191
tubes 247 T-bore stopcock 287 graduated pipettes 178
Separating funnels 275 Technical information 317-344 volumetric flasks 185, 187
holders for 275 Test and centrifuge tubes 245, 246, 271 UV-Cuvette 166
seripettor® Test tube racks 248
(bottletop dispenser) 33-38 Thermometers 229-236 V
seripettor® pro Anschütz- 233 Vacuum
(bottletop dispenser) 33-38 ASTM 235 desiccators 278, 279
Dispenser selection chart 36 cloud point and pour point 234 water jet pumps 282
Serological pipettes 181 congealing point 234 Valves, non-return 286
SILBERBRAND class B distillation (with standard ground Volumetric flasks 183-188
(information) 172 joint) 236 plastic 189
Silicone paste 288 dropping point 234 PUR plastic coated 186
Silicone stoppers 289 enclosed-scale trapezoidal 184
Slides pattern 230, 232, 236 USP 185, 187
boxes 260 high-temperature 233 with 3 marks, DAkkS calibrated 186
cover glasses for microscope slides 257 low-temperature 233 Volumetric instruments
microscope slides 257, 258 maximum 232 (information) 171-173
staining troughs 258, 259 precision 232-236
Soaking jar (rinsing apparatus for solid-stem 230-235 W
pipettes) 308 stirring 230 Wall mount for Transferpette® 57
Solid-stem thermometers 230-235 without mercury 231 Wall support
Spatulas 296 with standard ground joint 236 for accu-jet® pro 99
Splash heads (still heads) 304 Thoma counting chambers 255 for HandyStep® S 90
Spotting tiles 260 Threaded bottles with coating 299 Wash bottles 303
Spring clips 283 TipBox (pipette tips) 70-72, 74-81 Wash bottles, Gas 280, 281
Square bottles 299, 301 TipRack (pipette tips) 70, 72-77 Wash heads 303
Staining, troughs 258-260 TipStack™ (pipette tips) 71-75, 78, 79 Watch glasses 269
Stands Tips Water jet pumps 282
for bottles 26, 32 BIO-CERT®, rack packed 74-77 Water stills MonoDest 3000 310
for disposal bags 243 bulk and rack packed 74-77 Weighing dishes, disposable 298
for burettes 213 disposable pipette tips 68-82 Wide-mouth bottles 300-302, 306
for compact automatic burettes 213 PD-Tips 95, 96 Winkler oxygen flasks 219
for pipettes 309 Ultra Low Retention 78-81 Workstation storage/
rectangular base 213 with filter, bulk and rack packed 76, 77 dispenser boxes 142, 243
Starter-Kit Transferpette® S 50 Titrette® 39-44
Sterilization indicator tape 242 Trace analysis, Dispensette® TA 29-32
Stirrers Transfer pipettes (Pasteur-) 249, 250
magnetic stirring bars 292-295 Transferpette® S 45-50
bar retrievers 295 Transferpette® S -8/-12 51, 52
rods 296 Transferpette® 53-57
shaft 291 Transferpette®-8/-12 58, 59
spatulas 296 Transferpette® electronic 61-64
thermometers 230 Transferpette®-8/-12 electronic 65-67
with shaft 291 Transferpette® PipSet 57
Stopcock grease, silicone paste 288 Transferpettor 83-86
Stopcocks 202, 203, 211, 214, 278, Trays
287, 305 photographic 307
for aspirator bottles 305 pipette 309
for burettes 202, 203, 211, 214 Tissue culture graduated pipettes 182
needle valve stopcock for Tubes
desiccators 278 capillary 251, 252
straight-bore 287 centrifuge 245, 246
T-bore 287 coagulometer 247
conical ground joint 290 cryo 141
rubber 289 culture 245
silicone 289 rack 139
Storage bottles 305, 306 bulk 139
Straight-bore stopcocks (one-way) 287 sample 247
Strip plates 146, 152 sedimentation 247
Supports test 271
filter funnel 275 Tubing 283
for burettes 213 Tubing adapters
for funnels 275 (tubing connectors) 284-286
for Transferpette® S 52 Türk counting chambers 255
for Transferpette® 57, 59
www.brand.de 349
Index Number Index
350 info@brand.de
Number Index
Index
Cat. No. Page Cat. No. Page Cat. No. Page
4020 38 - 4020 46 188 7075 25 - 7075 37 44 7805 05 121
4520 00 - 4557 51 244 7075 42 32 7805 07 7805 25 120
4580 21 - 4587 34 276 7079 15 - 7079 18 26 7805 36 7805 50 121
4620 26 - 4632 43 277 7079 25 - 7079 26 27 7806 05 - 7806 08 125
4636 16 - 4661 34 276 7079 28 26 7807 00 - 7807 14 123
4700 45 - 4708 20 257 7079 30 27 7807 20 - 7807 24 124
4714 00 - 4718 00 260 7079 35 - 7079 38 26 7807 30 - 7807 34 123
4720 00 - 4727 00 258 7079 45 - 7079 56 32 7807 40 - 7807 44 124
4728 00 259 7084 40 - 7084 45 111 7807 50 - 7807 54 123
4731 00 258 7084 62 - 7084 72 112 7807 55 - 7807 75 124
4735 00 259 7086 05 252 7810 29 - 7810 40 245
4743 00 - 4743 05 260 7086 60 - 7091 09 251 7812 60 132
4744 00 - 4744 10 259 7091 10 252 7812 80 - 7812 94 129
4747 01 - 4747 44 257 7091 18 251 7813 00 - 7813 14 127
4755 05 - 4755 65 258 7091 22 251 7813 16 7813 27 128
4758 00 - 4769 00 260 7091 33 - 7091 44 251 7813 30 129
5770 38 - 5778 57 270 7178 05 - 7178 20 254 7813 32 - 7813 44 128
5778 91 - 5779 00 167 7180 05 - 7180 20 255 7813 45 - 7813 48 132
5780 00 213 7186 05 - 7186 20 254 7813 50 7813 54 130
7013 30 138 7189 05 - 7189 20 255 7813 57 131
7013 40 - 7013 54 137 7190 05 256 7813 58 - 7813 62 132
7013 55 - 7013 62 138 7195 05 - 7195 20 255 7813 64 7813 65 131
7013 64 - 7013 65 140 7198 05 - 7213 05 256 7813 66 7813 69 130
7013 67 133 7220 55 - 7220 60 240 7813 71 7813 78 131
7013 68 138 7230 14 - 7230 16 257 7813 80 7813 81 140
7013 70 7013 80 140 7320 02 - 7320 08 74 7813 90 - 7813 91 133
7015 01 - 7016 50 264 7320 10 - 7320 12 75 7814 00 131
7018 07 - 7018 78 85 7320 22 - 7320 28 74 7814 02 - 7814 05 133
7019 00 - 7019 68 86 7320 30 - 7320 32 75 7814 11 7814 16 129
7023 68 - 7024 04 96 7321 02 - 7321 08 74 7815 00 - 7815 83 139
7024 06 - 7024 38 96 7321 10 - 7321 12 75 7816 00 - 7816 14 146
7025 95 - 7026 08 75 7321 22 - 7321 28 74 7816 20 - 7816 27 147
7026 83 - 7026 96 96 7321 30 - 7321 32 75 7816 40 7816 42 148
7028 04 - 7028 12 85 7322 02 - 7322 08 74 7816 60 7816 71 146
7028 52 - 7028 90 86 7322 10 - 7322 12 75 7816 80 - 7816 87 147
7032 03 57 7322 22 - 7322 28 74 7817 00 7817 02 148
7032 08 57, 14 7322 30 - 7322 32 75 7817 20 - 7817 31 152
7032 10 57 7322 44 - 7322 48 74 7817 40 - 7817 42 153
7034 09 - 7034 11 67 7322 52 38, 75 7817 80 - 7817 89 152
7034 40 59 7322 64 38, 74 7818 00 - 7818 02 153
7034 59 67 7322 68 74 7818 40 - 7818 51 152
7036 00 - 7036 32 59 7322 72 38, 75 7818 60 - 7818 67 153
7037 00 - 7037 32 52 7323 02 - 7323 04 78 7819 00 - 7819 02 157
7039 70 - 7039 79 108 7323 06 44, 79 7819 04 - 7819 05 140, 157
7040 02 - 7040 20 299 7323 08 - 7323 12 79 7819 06 - 7819 71 157
7040 70 - 7040 75 57 7323 22 - 7323 24 78 7819 80 - 7820 02 158
7041 01 - 7041 82 56 7323 26 - 7323 32 79 7820 22 - 7820 31 157
7041 90 57 7323 44 78 7820 40 - 7820 47 158
7042 01 - 7042 08 27 7323 48 - 7323 52 79 7820 82 - 7820 91 157
7042 09 - 7042 72 28 7323 64 78 7821 00 - 7821 07 158
7042 75 26 7323 68 - 7323 72 79 7821 50 - 7821 53 164
7042 81 - 7042 82 28 7325 02 - 7325 04 76 7823 00 - 7823 01 146
7043 25 - 7044 31 26 7325 08 - 7325 14 77 7823 05 - 7823 06 152
7044 86 - 7044 95 27 7326 02 - 7326 06 76 7828 00 - 7828 91 162
7045 00 - 7045 08 37 7326 08 - 7326 14 77 8000 01 - 8001 04 230
7045 18 - 7045 20 38 7326 22 - 7326 26 76 8002 00 - 8002 08 231
7045 22 - 7045 23 37 7326 28 - 7326 34 77 8004 01 - 8006 48 230
7045 26 104 7327 02 - 7327 06 76 8040 02 - 8045 33 232
7045 32 - 7045 51 38 7327 08 - 7327 14 77 8050 01 - 8120 10 233
7045 54 - 7045 80 104 7327 22 - 7327 26 76 8130 49 - 8130 61 236
7046 52 7046 53 52 7327 28 - 7327 34 77 8206 00 232
7047 08 - 7047 93 50 7328 02 - 7328 10 80 8385 01 - 8385 03 231
7048 05 - 7048 10 52 7328 12 - 7328 14 81 8668 01 - 8711 01 234
7050 00 - 7050 53 94 7328 22 - 7328 30 80 8800 01 - 8800 90 235
7051 10 - 7051 30 90 7328 32 - 7328 34 81 9660 30 - 9685 23 225
7052 99 - 7053 03 32, 38, 64 7329 90 - 7329 96 74 9695 10 - 9696 16 226
7053 06 - 7053 07 38, 44, 64 7477 15 - 7477 20 250 9700 10 - 9705 10 225
7053 09 32, 38, 64 7477 50 - 7477 75 249 9715 28 - 9715 42 226
7053 10 - 7053 26 64 7493 11 - 7495 10 252 9803 10 - 9847 15 227
7053 27 32, 44, 64 7589 00 242 9874 02 - 9929 10 228
7053 29 7053 85 64 7589 01 - 7589 10 242 43400 00 - 43400 63 248
7053 86 57 7590 05 - 7591 15 167 43410 00 - 43410 53 125
7053 90 - 7053 93 64 7591 50 - 7592 43 166 47001 00 - 47003 61 24
7053 99 - 7054 36 66 7595 00 167 47201 20 - 47204 50 37
7054 49 - 7054 70 67 7597 00 - 7597 10 243 47231 50 104
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7060 86 7060 87 32 7780 12 - 7794 20 271
7060 90 26 7804 00 - 7805 02 120
www.brand.de 351
BRAND GMBH + CO KG General Terms and Conditions
1. General 5.6 The customer shall be required to inform us immediately of any attachments, seizures or any other third-
1.1. These General Terms and Conditions (GT&C) are intended for use in commercial transactions between party dispositions relating to the goods that are reserved or co-owned by us.
businesses. 5.7 In the event of failure to pay bills of exchange or cheques, or failure or recall of a payment made via SEPA
1.2. These GT&C shall apply for all, including future, contracts with the customer. Other terms and conditions Direct Debit Scheme, suspension of payments or insolvency of the customer or of the end buyer, the rights
shall not become part of the contract, even if we do not expressly object to them. Subsidiary agreements of the customer under Clause 5.3. shall no longer be valid. The customer must then immediately inform
made before or at the time of conclusion of contract may only be invoked if they are immediately confirmed the buyer of our extended retention of title; he may use the assignment of relevant proportional proceeds
in writing. The waiver of the requirement for written form shall only be possible in writing. The language only to pay for the delivered goods.
of the contract shall be German or English. 5.8 Where payment is delayed and in the cases covered in Clause 5.7, we shall be entitled to withdraw from
In the event of a discrepancy between the German language version of these GT&C and a version in any the contract and/or, without withdrawing from the contract, demand the return of any goods subject to
language, the German language version shall prevail. retention of title still in possession of the customer and to collect the assigned receivables ourselves. In
1.3. Our offers are subject to change and non-binding. We reserve the right to make technical improvements order to ascertain our rights, we shall be entitled to have all of our customer’s documents/books concerning
to our products. our reserved rights examined by a person who is subject to the professional duty of confidentiality.
1.4. We may store and process data in our IT system necessary for the purpose of processing the contract. 6. Warranty, Limitation of Liability
1.5. A set-off by the customer shall not be permitted unless the counterclaims are undisputed or legally 6.1 We warrant that our delivered goods (including any agreed installation) are free of defects at time of risk
established, or pecuniary counterclaims arising from the right to refuse payment pursuant to Section 320 transfer. The required quality, durability and use of our delivered goods are based solely on the agreed
Bürgerliches Gesetzbuch (BGB) (German Civil Code). written specification, product description and/or operating manuals. Any information beyond this, in particular
1.6. Orders with a goods value of below € 250 shall be subject to a minimum order surcharge of € 50. in preliminary discussions, advertising and/or referencing industrial standards shall only become part of
Delivery shall be undertaken generally in packaging units (PU) according to the currently valid price list. the contract if they are expressly referenced in writing.
For deliveries within five (5) working days or for order values up to € 500, we reserve the right to waive 6.2 Should the customer requires the delivered goods for purposes other than those agreed, he must take
an order confirmation. responsibility himself for examining their special suitability for this - also in terms of product safety - and
1.7. The place of jurisdiction shall be the court responsible for our head office in Wertheim/Mosbach, Germany. ensure their compliance with all relevant technical, legal or regulatory provisions before the intended use.
We shall also be entitled to appeal to the court responsible for the head office of our customer. We shall, We shall not be liable for any usability that was not expressly confirmed by us in writing. In the case of
furthermore, as plaintiffs have the right to invoke the Arbitration Court at the Chamber of Commerce and material or design requirements of the customer, we shall accept no liability for the suitability or permis-
Industry in Frankfurt am Main, Germany. The Arbitration Court shall, in this case, make the final judgment sibility of the desired materials or designs, and shall, in this respect, have no particular testing obligation.
in accordance with the Rules of Arbitration of the Chamber of Commerce and Industry in Frankfurt am Compliance with safety-related and occupational health regulations depends on the location and operating
Main without recourse to the ordinary courts of law. The instigation of legal dunning proceedings by us conditions of which we have no prior knowledge. Action for ensuring compliance shall therefore be the
shall not signify the exertion of our right of choice; it shall be admissible in all cases. responsibility of the customer or his buyer.
1.8. German law shall apply exclusively under the exclusion of the conflict of laws principles of Private International 6.3 We shall not be liable for the consequences of improper handling, use, maintenance and operation of
Law and the UN Convention on Contracts for the International Sale of Goods (CISG). the delivered goods; the consequences of normal wear and tear, in particular of wearing parts, such as
2. Delivery pistons, seals, valves; the breakage of glass, plastic or ceramic parts; for the consequences of chemical,
electrochemical or electrical influences; or non-observance of the operating instructions.
2.1 The place of performance shall be our factory in Wertheim, Germany. The risk shall transfer to the customer
when the delivery leaves the ramp at our factory. This shall also apply to partial deliveries or where we have 6.4 If a notice of defect is justified, we shall initially only be required to provide supplementary performance.
performed additional services, such as shipping; costs for transport, packaging or insurance; exportation Supplementary performance shall be, at our discretion, either rectification of the defect or delivery of goods
and installation. free of defects. Further warranty claims shall only apply in the event of rejection, impossibility or failure of
the supplementary performance. The customer shall bear additional expenses, which arise from the fact
2.2 Insofar as we have agreed to orders on call, the customer must take delivery of the total amount within
that the goods were taken after delivery to a location other than the agreed place of performance.
six (6) months, at the latest at the date confirmed by us.
6.5 The customer must, immediately upon receipt of the goods, inspect them carefully, also in terms of product
2.3 In the case of a delay in the customer’s acceptance of a delivery, we may, without prejudicing our claim
safety, and notify obvious defects immediately in writing; any hidden defects must be immediately notified
for performance, have the goods put into storage at the cost of the customer or, after providing a warning
upon discovery. The customer must notify the carrier immediately of any transport damage. Failure to
and setting a deadline for the customer, otherwise dispose of them.
observe the testing and notification obligation shall void any customer claims for defects.
3. Delivery Period, Delay 6.6 Our liability for slight negligence shall be limited to claims for injury to life, limb or health, to claims under
3.1 Delivery times shall be ex works. Delivery periods shall begin on receipt of our order confirmation by the Produkthaftungsgesetz (German Product Liability Act) or to claims of culpable breach of fundamental
the customer; however only after settlement of any technical issues pending from the conclusion of the contractual obligations through which the purpose of the contract is endangered. Otherwise, our liability
contract; and after receipt of any documents to be provided to us by the customer, such as drawings, for slightly negligent breach of fundamental contractual obligations is limited to the typically occurring
permits or approvals; and definitely not before receipt of agreed advance payments. The delivery period damages which we could have foreseen when the contract was concluded.
shall be considered to have been met if readiness for dispatch has been notified before the expiry of this 6.7 Should the customer use the delivered goods in conjunction with environmentally harmful, toxic, radioactive
period. Delivery shall be subject to us receiving our own supplies punctually and in good order. or otherwise hazardous materials, he shall be obliged to clean them before returning them to us. If appli-
3.2 Force Majeure and circumstances beyond our control, such as strikes, lock-outs, operational disruption, cable, we may charge any necessary costs for decontamination/cleaning and disposal to the customer’s
shortages of raw materials and equipment, delayed delivery or non-delivery by our suppliers, shall extend account.
the delivery periods accordingly and shall release us from our delivery obligations if they, as a result, render
7. Limitation Period
delivery impossible. We shall also not be liable for the circumstances described above if they arise during
an already existing delay. The same shall apply for any additional or amended services requested by the The warranty period shall be one year and starts from the date of delivery of the goods to the customer. The
customer. same shall apply for claims for damages, irrespective of their legal basis. The limitation periods of Section 438
Para. 1 Nos. 1 and 2, Section 479 Para. 1 and Section 634a Para. 1 No. 2 of the BGB (German Civil Code)
3.3 We shall be considered to be in default of delivery only if the customer has issued us with a reminder, has
shall remain unaffected. The restriction of the limitation period shall not apply to claims based on fraudulent
set a reasonable extension period which has elapsed.
concealment of a defect, for claims under the Produkthaftungsgesetz (German Product Liability Act) or for
3.4 In the case of delay damages, our liability for compensation shall be limited to 10% of the value of the damages resulting from injury to life, limb or health and other damages based on intent or gross negligence.
delayed delivery/service. The limitation shall not apply in cases of wilful intent, gross negligence and/or The limitation period in respect of replaced or repaired goods shall not commence anew.
injury to life, limb or health. The customer shall be obliged to immediately inform us in writing of any likely
consequences of delay. 8. Software Use
8.1 If software is included in the scope of a delivery, the customer shall be granted a non-exclusive right to
4. Prices, Terms of Payment
use the software and its associated documentation. It is provided for use on the designated delivery item.
4.1 Prices shall be EXW (Incoterms® 2010 ex works), Wertheim and exclusive of statutory VAT, if applicable. The use of the software on more than one system shall be prohibited.
Costs of packaging, transportation, freight and insurance shall be borne by the customer. Prices shall also
8.2 The customer shall only be entitled to copy, transfer or translate the software or to convert it from object
be exclusive of the cost of returning and recycling/disposing of old equipment.
code to source code to the extent permitted by law (Sections 69a et seq. Urheberrechtsgesetz – German
4.2 Invoices shall be payable to our account in EUROs (€) without deductions and free of charges and expenses. Copyright Act). The customer undertakes to refrain from removing manufacturer information, in particular
Payment shall be made immediately or by the date stated. The determinant factor shall be the receipt of copyright notices, or from changing these without our prior express consent or the prior express consent
payment. Cheques and bills of exchange shall only be accepted on account of performance and at the of the software supplier.
cost of the customer.
8.3 All other rights to the software and the documentation including copies thereof shall remain with us and/
4.3 In the case of customers, with whom we are working for the first time or with whom we do not work regularly, or the software supplier. The issue of sub-licences is not permitted.
after delay in payment or in the case of reasonable doubt as to the creditworthiness of the customer, we
shall reserve the right to make individual deliveries dependent on a pre-payment or a security deposit to 9. Installation
the value of the invoice amount. 9.1 Installation costs may be invoiced on a monthly basis. Fixed installation prices shall only cover the work
4.4 Should the period between conclusion of contract and agreed delivery exceed four (4) months, so may that has been agreed upon. In other cases our current price list for installation and service costs shall
we, at our discretion, demand a reasonable additional charge equivalent to the increase in our costs up apply.
until delivery. For deliveries on call, our current price shall apply. 9.2 The customer shall be responsible for providing the following at his own expense: lighting, motive power,
4.5 In the case of an agreed return of goods that are free of defects, the customer shall be charged a checking if necessary, compressed air; water; electrical power for welding and heating, including the necessary
and processing fee of 20% of the invoice amount (minimum € 50). connections; electrical installations to connect the products supplied by us; the devices required (such as
lifting equipment); a lockable room that can be used for storing materials; tools and clothing during the
4.6 Should the customer be in arrears with payment, our debt claims against him shall be due immediately,
installation.
and we shall not be obliged to make any further deliveries based on current delivery contracts.
4.7 If payment is delayed, we shall charge - notwithstanding further damage compensation claims - interest 10. Spare Parts, Maintenance/Repair and Calibration
on arrears at the statutory rate. 10.1 For spare parts and maintenance, repair and calibration services, the current repair and exchange price
4.8 We may offset amounts payable to the customer (e.g. from credit notes) against our claims against the list shall apply.
customer. 10.2 Insofar as there is an obligation on our part to maintain/supply spare parts, then this obligation shall be
limited to a period of five (5) years from the date of delivery. If spare parts are not manufactured by us, or
5. Retention of Title, Assignment of Future Claims
are no longer available on the market, for example electronic components, or if the raw material for their
5.1 The goods delivered shall remain our property until the complete and unlimited payment. Should we still
production is no longer available, our obligation to deliver spare parts shall lapse.
have further claims against the customer, we shall then retain our property rights until payment of these
10.3 For calibration and maintenance, expendable items from BRAND production are normally used.
has been effected.
10.4 Maintenance and calibration services can only be provided if the customer has declared the devices sent
5.2 The customer may neither use goods subject to retention of title nor combine them with other objects to
to be safe to work on from a health hazard perspective.
which a third party may have rights. Should, however, goods subject to retention of title become, through
their combination with other objects, part of a new (complete) item, we shall be a proportional co-owner 10.5 For repair/service values of up to € 50, we reserve the right not to provide a separate cost estimate.
of this new item directly, even if this latter component is regarded as the main component. Our proportion 11. Legal Reservation, Industrial Property Rights, Confidentiality
of co-ownership shall be determined by the ratio of the invoice value of the goods to the value of the new 11.1 We reserve ownership and all industrial property rights and copyrights to all moulds, tools or other devices,
item at the time of combination. samples, pictures, and business and technical documents produced or provided by us. This also applies
5.3 The customer may resell goods subject to retention of title in the course of his normal business as long where the customer has wholly or in part taken on the costs hereof. The customer may use these only
as he has not assigned, pledged or otherwise encumbered his claims from the resale. in the manner agreed with us. Without our written consent, he may not himself manufacture contractual
5.4 The customer shall assign to us in advance as collateral any claims against his customers from the resale objects delivered or have the same manufactured by third parties.
of the goods subject to retention of title (see Clause 5.3) and/or newly formed items (see Clause 5.2) 11.2 Insofar as we deliver goods according to the designs or other requirements specified by the customer
to the value of our invoice for the goods subject to retention of title. As long as the customer is not in (models, patterns etc.), the customer shall be liable to us by default for ensuring that, through the manu-
default of payment for the goods subject to retention of title, he may collect the assigned claims in the facture and delivery of these goods, the industrial property rights or other rights of third parties are not
ordinary course of business. He may, however, only use the proportional proceeds for the payment to us infringed. If the customer is at fault he shall reimburse us all damage resulting from any such infringement
for the goods subject to retention of title. of rights.
5.5 At the customer’s request, we shall release collateral at our discretion if and to the extent that the nominal 11.3 Any information acquired from this business relationship and not deemed to be public knowledge must not
value of the collateral exceeds 120% of the nominal value of our outstanding debt claims against the be disclosed by the customer to third parties. Status as of: September 2014
customer.
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