Quality Assessment of Fruit Juices
Quality Assessment of Fruit Juices
College of Education
Department of Food Nutrition and Science
Submitted By:
AGUILAR, Cristelle Angela R.
GALANIDO, Shenaiah Luisa S.
LARGO, Heaven Dane C.
MARCELO, Andrea Marie M.
YU, Jazlyn Bon O.
Submitted To:
Ms. Eiselle Joyce R. Hidalgo
METHODS:
Specific Gravity – to determine the fluid’s characteristics compared to water
Instrument Used:
mass.
Reagents Used:
and an indicator which helps in determining the vitamin c content. It changes its
Oxalic Acid – is an organic compound used to stabilize the ascorbic acid. Oxalic
acid also the ascorbic acid in its oxidation through ascorbic acid oxidase.
Instrument Used:
Instrument Used:
Titratable Acidity – to determine the total amount of the acid (malic, citric, tartaric)
in the juices
Reagents Used:
in the sample.
alkaline.
TSS (Total Soluble Solids) – to determine the amount of solids dissolved in the
juices.
Instrument Used:
acceptability) – to evaluate and interpret the juices through the sense of sight,
pH
hydroxyl ions present (Loftus, 2003). The reasons for determining pH in food processing
produce products with reliable precise properties, and to avoid causing health problems
to consumers (Deshpande et al., 2015). The principle of the pH meter is to measure the
hydrogen ions. The greater this concentration of hydrogen ions, the stronger the acid is.
is approximately between 3.35-4 pH, for zesto orange juice is approximately 3.20-4.19
pH, and for zesto grape juice is approximately 2.90-3.25 pH. Table 1 shows the pH results
for zesto apple, orange and grape juices having the values of 3.39, 3.25 and 3.05
respectively. These three samples have reached the set US FDA standard pH level and
The principle of total soluble solids (TSS) is the dry soluble solids content of a
sample is estimated from its refractive index, with reference to the refractive index of a
pure sugar solution (Badilla and Manara, 2014). The one used in the experiment is a hand
refractometer.
In Table 1, the standard measurement of the total soluble solid for zesto apple
juice and zesto orange juice should not be below 10oBrix (CODEX STAN 247, 2005) while
for zesto grape juice should not be less than 16oBrix (CODEX STAN 247, 2005). The
results for zesto apple and orange juice gave a value of 10.53 oBrix and 10.33oBrix
respectively and has acceptable total soluble solid content since the values are not lower
than 10oBrix while for zesto grape juice gave a value of 10.20 oBrix and it did not comply
Specific Gravity
Specific gravity is the ratio of the density (mass of a unit volume) of a substance
to the density (mass of the same unit volume) of a reference substance. One approach
According to FAO-USDA (2012), the standard specific gravity of zesto apple and
orange juice is 1.05 while for zesto grape juice is 1.07. Based on Table 1, the specific
gravity calculated in the experiment for zesto apple juice was 0.9472, for zesto orange
juice was 0.9555 and for zesto grape juice was 0.9584. All three juice samples are close
to their standards.
The main constituent of juice is water which accounts for 70 to 97 percent juice.
Water content in fruits is mostly influenced by cultivation and post - harvest conditions.
Juices are classified according to total soluble solids present, juice content and kind of
processing used (George and Moiloa, 2015). The moisture content of zesto orange,
apple, and grape are 88.86%, 88.18%, and 88.34% respectively which are all within the
Titrable Acidity
acids present in a known quantity of food sample using a standard base. Its endpoint is
phenolphthalein, and the volume of titrant used along with the normality of the base and
the weight of sample used are used to calculate the percent titratable acidity expressed
in terms of the principal organic acid. Titratable acidity quantifies a food’s total acid
concentration, mostly organic samples. Organic acids naturally present in some food can
also be formed and/or added to a specific food formulation due to its contribution in
enhancing the flavor of food through this method. Titratable acidity can also be used as
an index of maturity for some fruits along with the sugar content of the commodity
(Nielsen, 2010). The titratable acidity of the zesto orange, apple, and grape juices are
0.25%, 0.18%, and 0.23% respectively. It infers that only a small amount of possibly
naturally occurring acids (citric, malic, and tartaric acids) are present in the juices.
Vitamin C Analysis
color is observed indicating that the solution contains excess unreduced dye (Nielsen,
2010). The purpose of this analysis is to monitor the amount of ascorbic acid present in
certain food products, especially those that were affected by many factors including
climate, harvesting methods, storage, and processing (Gazdik et al., 2008). In order to
al., 2008) in order to prevent diseases such as scurvy (Kumar et al., 2013), which is why
most food industries are increasing the ascorbic acid content of their products especially
fruit juices to be able to maintain the body’s necessary dose of vitamin C even after
processing, resulting to the application of this analysis (Majidi and Y-ALQubury, 2016).
According to the Philippine National Standard (PNS) for citrus beverage products (2010),
ascorbic acids maximum level in the juice should be 200mg/kg. The zesto orange juice
was at 107.63mg/kg and did not went above the mentioned limit meaning that they were
Sensory Evaluation
The principle involved in this method of analysis is the utilization of the perceiving
power of the five senses namely sight, smell, touch, taste, and hearing in order to analyze,
measure, and interpret sensations as well as the characteristics of food and materials
(Stone and Sidel, 2004). This type of analysis converts the subjective nature of human
statistics, etc (Gatchalian and Brannan, 2011). Sensory evaluation controls the effect from
changes or modifications in the process flow of certain product starting from raw handling
until packaging. It helps identify sensory attributes that drives consumer acceptance at
the same time the quality of the product (Kemp et al., 2009). According to the Philippine
National Standard (PNS) for citrus beverage products (2010), as long as the taste and
odor are unobjectionable, then the fruit juice has passed these two parameters. Color and
turbidity of the fruit juices are not measured through sensory evaluation but rather
mechanically in the PNS. The sensory panelists concluded that no off-flavors and aroma
was noticed in the fruit juices, thus all are acceptable under the Philippines standards.
IV. CONCLUSION
Fruit juices are highly complex food products with a wealth of valuable
ingredients. Before the product goes out of the market, set of standards are arranged to
make sure that the product is safe to consume by people. In this experiment, three fruit
juices (Orange, Apple, and Grapes) are used in testing the quality assurance standards
set by the US FDA. The three juice samples have reached the standards of the quality
parameters of pH that was set by the US FDA. The zesto orange juice and the zesto
apple juice gave an acceptable total soluble solid content, however, the zesto grape juice
did not comply with the standards. All three juice samples’ specific gravity was somehow
close to the given standards, making them rejected in terms of specific gravity. Moisture
content of each juice are within the moisture content range of 70-97%. The three juice
samples inferred only small amount of natural occurring acids based on titratable acidity.
And as for the % ascorbic acid content found in the zesto orange juice, it did not go
beyond the standard set by the PNS. The quality parameters total soluble solids, specific
gravity, and % titratable acid are out of range. Therefore, the zesto orange juice, zesto
apple juice, and zesto grape juice are rejected for not reaching the set of standards for
quality assurance of fruit juices. All three juice samples showed no off-flavor and aroma
Badilla, M., & Manara, E. (2014). Titratable acidity and total soluble solids. International
Deshpande, S.A., Yamada, R., Mak, C., Hunter, B., Obando, A., Hoxha, S., & Ja,
W.W. (2015). Acidic food pH increases palatability and consumption and extends
Gatchalian, M.M., & Brannan, G.D. (2011). Sensory quality measurement: Statistical
Company, Ltd.
Gazdik, Z., Zitka, O., Petrlova, J., Adam, V., Zehnalek, J., Horna, A., Reznicek, V.,
Beklova, M., & Kizek, R. (2008). Determination of vitamin c (ascorbic acid) using
Sensors, 8, 7097-7112.
George, M., & Moiloa, L. (2015). Determination and comparison of physico – chemical
Kemp, S.E., Hollowood, T., & Hort, J. (2009). Sensory evaluation: A practical handbook.
Kumar, G.V., Ajay, K.K., Raghu, P.G.R., & Manjappa, S. (2013). Determination of vitamin
Nielsen, S.S. (2010). Food analysis, (4th ed.). New York, NY: Springer Science &
Business Media.
Nielsen, S.S. (2010). Food analysis laboratory manual, (2nd ed.). New York, NY: Springer
Stone, H., & Sidel, J.L. (2004). Sensory evaluation practices, (3rd ed.). Cambridge, MA:
Academic Press.
APPENDICES
Raw data for the computation of pH
Lot A Lot B Lot C pH
Grapes 3.010 3.07 3.06 3.0466
Orange 3.24 3.25 3.27 3.2533
Apple 3.37 3.43 3.37 3.39
= 0.2477142577%
Computation for Titratable Acidity of Apple Juice
%TA = (Ntiter x Vtiter)(meq. wt. of acid) x100
wt. of sample
Trial 1 (3.00𝑚𝑙)(0.1𝑁)(0.064)
% Malic = 𝑥 100
10.35𝑔
=0.1942028986%
Trial 2 (2.90𝑚𝑙)(0.1𝑁)(0.064)
% Malic = 𝑥 100
10.68𝑔
=0.181928839%
Trial 3 (2.40𝑚𝑙)(0.1𝑁)(0.064)
% Malic = 𝑥 100
10.20𝑔
=0. 1776470588%
Average:
0.1942028986+0.181928839+0.1776470588
= 3
= 0.1845929321%
Standardization of KHP
Trial 1 Trial 2