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Microsoft Word - Restriction Enzymes Worksheet

The document provides examples of restriction enzyme digestion of DNA sequences. It identifies restriction sites, number of DNA fragments produced, and fragment sizes for enzymes HpaI, SspI, PstI, HindII, EcoRI, HaeIII, BamI alone and in combinations.

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2K views2 pages

Microsoft Word - Restriction Enzymes Worksheet

The document provides examples of restriction enzyme digestion of DNA sequences. It identifies restriction sites, number of DNA fragments produced, and fragment sizes for enzymes HpaI, SspI, PstI, HindII, EcoRI, HaeIII, BamI alone and in combinations.

Uploaded by

Haru
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Restriction Enzymes Worksheet Name: ___________________________

Objective(s):

 Identify restriction sites.


 Show differences between blunt and sticky (cohesive) end cuts.
 Compare restriction enzyme differences on identical DNA.

Directions: Identify the restriction sites for each of the examples given. Show the cuts , sticky
(cohesive) or blunt, number of DNA fragments produced and the number of base pairs in each
(count the top row). (If there are three nucleotides on either side of the dash it is a blunt cut. If there are
less than three on either side of the dash it is cohesive cut.)

1. HpaI --- 5' GTT - AAC 3'

 5' GGATGTTAACAATCTCTACGGGTTAACACCCTTGGGTTAACATCCGCGG 3'


 3' CCTACAATTGTTAGAGATGCCCAATTGTGGGAACCCAATTGTAGGCGCC 3'

Number of pieces of DNA _______


2. SspI --- 5' AAT - ATT 3'

 5' GGATAATATT GTTAACAATCTCTACGGGTTAACACCCTTGGAATATTTTAA 3'


 3' CCTATTATAACAATTGTTAGAGATGCCCAATTGTGGGAACCTTATAAAATT 3'

Number of pieces of DNA _______


3. PstI --- 5' CTGCA - G 3'

 5' ACGCTGCA/GACGTATTATTAT CCGCCGCTGCA/GCC GTCATCA 3'


 5' TGCG/ACGTCTGCATAATAATAGGCGGCG/ACGTCGGCAGTAGT 3'

Number of pieces of DNA _______


4. HindII --- 5' GTC - GAC 3'

 5' ACGACGTAGTCGACTTATTAT GTCGACCCGCCGCGTGTCGACCATCA 3'


 5' TGCTGCATCAGCTGAATAATACAGCTGGGCGGCGCACAGCTGGTAGT 3'

Number of pieces of DNA _______


5. EcoRI --- 5' G - AATTC 3'

 5' ACG ACGTATTAGAATTCTTAT CCGCCGCCGGAATTCT CATCA 3'


 5' TGC TGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT 3'

Number of pieces of DNA _______


6. HaeIII --- 5' CC - GG 3'

 5' ACGCCGGCCGTATTAT CCGGATCCGCCG CCGGCTGTCCCGGATCA 3'


 5' TGCGGCCGGCATAATAGGCCTAGGCGGCGGCCGACAGGGCCTAGT 3'

Number of pieces of DNA _______


7. BamI --- 5' CCTAG - G 3'

 5' ACGCCTAGGACGTATTATCCTAGGTAT CCGCCGCCGT CATCA 3'


 5' TGCGGATCCTGCATAATAGGATCCATAGGCGGCGGCAGTAGT 3'

Number of pieces of DNA _______


8. HpaI --- 5' GTT - AAC 3' and SspI --- 5' AAT - ATT 3'

 5' AGTTAACCGACAATATTGTATTATATCC GCCGCC GTCGTTAACATCA 3'


 5' TCAATTGGCTGTTATAACATAATATAGGCGGCGGCAGCAATTGTAGT 3'

Number of pieces of DNA _______


9.HindII --- 5' GTC - GAC 3' and HaeIII --- 5' CC - GG 3'

 5' ACGGTCGACACGTATTATTAGTCGACTCCGCCGCCGCCGGTCATCA 3'


 5' TGCCAGCTGTGCATAATAATCAGCTGAGGCGGCGGCGGCCAGTAGT 3'

Number of pieces of DNA _______


10. HindII --- 5' GTC - GAC 3', HaeIII --- 5' CC - GG 3' and BamI --- 5' CCTAG - G 3'

 5' ACGCCGGACGTACCTAGGTTTAGTCGACTC CGCCG CCCCTAGGGTCATCA 3'


 5' TGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 3'

Number of pieces of DNA _______

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