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Transcription Q&A

The document contains multiple choice questions about transcription. It tests knowledge about the key parts of transcription including: - RNA polymerase is the enzyme that synthesizes RNA under the direction of DNA. - The products of transcription include mRNA, tRNA, and rRNA. - The synthesis of mRNA from a DNA template is called transcription. - The recognition site on DNA that RNA polymerase binds to initiate transcription is called the promoter. - An mRNA transcript contains a start codon, stop codon, and terminator sequence. - The σ subunit of RNA polymerase facilitates recognition of promoter sequences.

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Gabriel Delos Reyes
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1K views7 pages

Transcription Q&A

The document contains multiple choice questions about transcription. It tests knowledge about the key parts of transcription including: - RNA polymerase is the enzyme that synthesizes RNA under the direction of DNA. - The products of transcription include mRNA, tRNA, and rRNA. - The synthesis of mRNA from a DNA template is called transcription. - The recognition site on DNA that RNA polymerase binds to initiate transcription is called the promoter. - An mRNA transcript contains a start codon, stop codon, and terminator sequence. - The σ subunit of RNA polymerase facilitates recognition of promoter sequences.

Uploaded by

Gabriel Delos Reyes
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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Which of the following enzyme is used for synthesis of RNA under the direction of DNA?

A. RNA polymerase

B. DNA ligase

C. DNA polymerase

D. RNA ligase

Answer: Option A

Which of the following is a product of transcription?

A. mRNA

B. tRNA

C. rRNA

D. all of these

Answer: Option D

The synthesis of mRNA based on a DNA template is called

A. DNA replication

B. transcription

C. translation

D. DNA restriction

Answer: Option B
Recognition/binding site of RNA polymerase is called

A. receptor

B. promoter

C. facilitator

D. terminator

Answer: Option B

Recognition/binding site of RNA polymerase is called

A. receptor

B. promoter

C. facilitator

D. terminator

Answer: Option B

An mRNA transcript of a gene contains

A. a start codon

B. a stop codon

C. a terminator

D. all of these

Answer: Option D
The components found in all prokaryotic transcription terminators is

A. a poly-U region

B. Rho factor

C. a hairpin structure

D. none of these

Answer: Option C

A specific __________ factor helps RNA polymerase bind to late promoters and transcribe late genes.

A. sigma

B. beta

C. delta

D. gamma

Answer: Option A

Which of the following component of RNA polymerase facilitates the recognition of promoter
sequences?

A. a subunit

B. b subunit

C. s subunit

D. c subunit

Answer: Option C

Like replication, transcription also occurs bidirectionaly


A True

B False

Ans: False

Explanation: Transcription occurs unidirectionally and synthesized RNA chain from 5’ to 3’ direction

Mark the statement which is INCORRECT about the transcription unit?

a) It is a transcribed segment of DNA

b) Eukaryotes have monocistronic transcription unit

c) Prokaryotes also have a monocistronic transcription unit

d) Immediate product of transcription is primary transcript

Answer: c

Explanation: Transcription unit is the transcribed segment of DNA. If the transcription unit carries
information of one gene, it is called monocistronic while a set of adjacent genes when transcribed as a
unit called polycistronic.

How many base pairs of DNA is transcribed by RNA polymerase in one go?

a) 5-6

b) 3

c) 4

d) 7-8

Answer: d

Explanation: The RNA polymerase core enzyme moves along the template strand at uneven base i.e,
moves over 7-8 base pairs in one go.

True or False nalang ni


The sole function of RNA is translation to a protein product

(True of False)

Ans: False

Halos same lang ogdi sa baba

Which of the following is not true about RNA?

Possible Answers:

RNA contains uracil

The sole function of RNA is translation to a protein product

RNA contains a five-carbon sugar

RNA contains adenine

RNA can be double stranded

Correct answer:

The sole function of RNA is translation to a protein product

Explanation:

There are a few different types of RNA, each serving different purposes. Messenger RNA (mRNA) is
transcribed to a protein product, but transfer RNA (tRNA) acts as a carrier for amino acids while
ribosomal RNA (rRNA) forms some structures of the ribosome. Micro RNA (miRNA) can be used to
regulate transcription.
RNA can be single or double stranded, leading to both ssRNA and dsRNA viruses. The ribose sugar in
RNA forms a five-carbon ring, much like deoxyribose in DNA. RNA contains both adenine and uracil,
though thymine is not found in RNA.

What enzyme is required for transcription of mRNA in eukaryotes?

Possible Answers:

RNA polymerase III

RNA polymerase II

DNA polymerase

RNA polymerase I

Correct answer:

RNA polymerase II

Explanation:

RNA polymerase II is required for transcription of mRNA, snRNA, and miRNA. Alternatively, RNA
polymerase I transcribes some rRNA and RNA polymerase III transcribes tRNA, some rRNA, and other
small RNAs. DNA polymerase is required for DNA replication but does not play a role in transcription.

Which class of upstream DNA element is responsible for increasing transcription of target genes?

Possible Answers:
Activator

Enhancer

Repressor

Insulator

Correct answer:

Enhancer

Explanation:

An enhancer is a cis-acting element that is responsible for activating or increasing expression of a target
gene. An insulator is a boundary element between inactive and active domains of DNA. Both activators
and repressors are trans-acting (protein) factors that modulate gene expression.

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