SPHERA DUO

• Random access instrument

• Reusable cells with wash station

• 200-280 tests per hour

• Sample barcode

• Can use primary tubes

• Innovative Hi software

• 2 pipetting arms

• 60 reagent positions

• Easy maintenance

• Handling of urgencies

By doubling the number of sampling arms

Sphera Duo increases the throughput of Sphera,
reduces reagent contamination and
thus makes it better suited for laboratories
with large testing volumes.
 SPHERA DUO
Technical
Operation features
specifications
PIPETTING Volume: sample, 2 - 300 µl; reagent, 5-350 µl
Precision: 1.5 CV% at 2 µl; 1 CV% at 4 µl
Physical characteristics Mixing by sample needle upon dispensation

SIZE / WEIGHT Height: 49 cm (19.3 in) Weight: 48 kg (105.8 lbs) REACTION Reaction volume, 210 - 350 µl
Depth: 62 cm (24.4 in)
Width: 85 cm (33.4 in) SAMPLE In-needle dilution if allowed by method’s sample volumes
DILUTION Automatic pre-dilution in a reaction cuvette, up to 1:100
POWER SUPPLY 240 / 100 Vac, 50 / 60 Hrz, single phase with ground
Independent on-off switch for refrigerated reagent plate TEMPERATURE Reagent refrigeration, circa 12 °C below room temperature
Fuse compartment / fuses: 2 Amp @ 230 Vac, 4 Amp @ 115 Vac CONTROL Reaction cells, heating unit can be set from
Power consumption: less than 200 VA (external PC excluded) room temperature up to 42 °C ±0.2 ºC (108 °F ±0.5 ºF)
Ground resistance: less than 0.1 Ohm
Leakage current: less than 2.5 mA TYPES OF TESTS Endpoint, Bichromatic endpoint, Differential endpoint,
Differential endpoint sample blank, Fixed Time, Kinetic
SAMPLING ARMS 2 sampling needle,110 mm needle stroke
Capacitive liquid level detector TEST RUNS Random / Urgent
Needle shock sensor
MEASUREMENT 200 tests/hour for double reagent run
DILUTER SYRINGE Long life plunger RATES 280 tests/hour in single reagent mode
Syringe capacity, 368 µl Maximum incubation + reading time: 638 seconds
Syringe resolution, 0.14 µl Typical precision, endpoint 2.0 CV% / kinetic 2.0 CV%
Carry-over, lower that 15 parts per million
HYDRAULIC 10 self-priming peristaltic pumps (life 1000 hrs)
SYSTEM with replaceable neoprene cassette (life 500 hrs) START-UP The start-up procedure is run daily: self-test,
2 vacuum pumps reader offset of optics, wash and check of all cuvettes
2 pinch valves
Manifold CALIBRATION Reagent blank subtraction, 1 to 8 standards per test method
Containers*: Water, 20l; Cleaning solution, 2l; Waste, 20l Linear: factor, linear, linear regression (standard’s repetitions)
* equipped with level sensor and safety connections Non linear (3 interpolation types): cubic-spline, poly-linear
and logit-log four parameters
WASH STATION Needles: 6 dispensing, 6 aspiration, 1 cleaning Free standard / control positions on all the sample plate
(8 step washing sequence for each cuvette) Results can be recalculated when changing factor or curve

REAGENTS TRAYS 2 removable racks MAINTENANCE Procedures programmed by component life counters
30 + 30 bottles, 50 ml or 24 ml (up to 3000 ml total)
PRINTING Single test, complete sample, work sheet, method and QCs
SAMPLES TRAY (Sphera standard) Removable tray, 60 numbered positions, REPORTS Automatic sample reports upon test completion if requested
tubes of 12 - 13 mm, 5 - 7 ml / cups of 0.5-1.5 ml
(cups require a metal adapter for level detection) NEEDLE Sampling needle washed internally and externally
(Optional) Removable tray, 20 + 20 numbered positions, WASHING with system solution after every operation
20 tubes of 12 - 16 mm / 20 cups (3,5 ml type)

CUVETTE ROTOR 80 washable BIONEX® cuvettes

REACTION CELLS which allow up to 30 000 tests per rotor
Optical path 6 mm, 210 - 350 µl reaction volume
Connections
100W heating resistance, temperature sensor, safety thermostat POWER Standard VDE removable power cord

OPTICAL GROUP 1 halogen lamp (6 V, 10 W) with extended UV emission EXTERNAL PC USB port
2 focusing lenses, optical glass
10-position filter disk: 8 positions provided with interference filters HOST/ LIS Ethernet LAN (samples, work list, results)
of 340, 405, 505, 546, 578, 600, 650, 700 nm wavelengths, Standard ASTM ASCII protocol
1 free position and 1 solid position for dark reading
Direct reading reaction cuvettes, 6mm optical path
±2 nm on peak wavelength, band pass of ±10 nm
Database
PHOTOAMPLIFIER Photoelectric detector
Signal amplifier WORKLIST/ For each worklist: unlimited number of samples, unlimited
Response range, 340 nm to 900 nm SAMPLES number of tests, up to 99 sheets of tests per worklist.
Photometric range, 0 to 2.5 Abs Sample-patient ID codes
Linearity, ±0.5% full scale
Precision: 0.5 CV% (0.050 to 1.500 Abs) TEST METHODS Unlimited number of methods in PC memory
Stability: daily reader offset, less than 1% drift per day 40 active methods

CONTROL Real-time multitasking microprocessor based control QUALITY Three-level controls per test, one month monitoring
Easy access to the electronics CONTROL Reagent/calibrator/control lot monitoring,
Exclusion of failing results from graphic and statistics
EXTERNAL (Minimum requirements) Intel Pentium IV family
COMPUTER with 2 GHz, 20 Gb HD, 512 Mb ram, CD/R ERROR LOG Automatically stored at run-time, can be viewed or printed
17” monitor, 1280 x 1024 resolution Powerful on-line monitoring
Keyboard and mouse
USB port
Windows® 7 with .NET framework 4.0
Edif Instruments S.r.l.
17” LCD touch screen monitor, 1280 x 1024 resolution via Ardeatina, 132 - 00179 - Rome, Italy
LAN port for LIS host communication Phone: +39 06 5127161 - Fax: +39 06 5127550
External printer, A4
Web: www.edif.it - Email: info@edif.it