BIOCHEMISTRY TEST BLUE PRINT

OBJECTIVES RECALL COMPRE- ANALYSIS/ QUESTION NO.

HENSION PROBLEM
SOLVING
A. The Cell
1. To discuss the different structures of the cell & their 1 29
respective functions.
B. Properties of Water
1. To describe the ion product of water. 1 1
2. To discuss the different properties of water. 1 5
C. Acid Base Equilibrium
1. To discuss the different physiological/clinical condi- 1 2
tions resulting in acid base imbalance
2. To describe the buffer systems and their role in the 1 1 3,4
maintenance of acid-base balance
D. Integration of Metabolism
1. To discuss the CORI cycle 1 40
2. To describe the interelationships of the different 2 50, 86
pathways of metabolism
3. To discuss the role of cyclic AMP in metabolism. 1 68
E. Bioenergetics
1. To discuss the different potential sources of ATP 1 1 70, 71
production
2. To discuss the different drugs that can inhibit oxida- 1 74
tive phosphorylation of ATP
F. The Electron Transport Chain
1. To discuss the different processes involved in the 1 1 64, 67
electron transport chain
2. To describe the role of flavoproteins in the electron 1 65
transport chain.
3. To discuss the different inhibitors of the electron 1 66
transport chain.
4. To discuss the connection between oxidative phos- 1 69
phorylation and electron transport chain.
G. Enzymes
1. To discuss the importance of enzymes in clinical 1 7
diagnosis and treatment of diseases.
2. To describe the basic structure of an enzyme. 1 1 33, 80
3. To discuss the different diseases associated with 1 2 41, 42, 43
enzyme defficiencies.
4. To discuss the differet function of enzyme. 2 57, 89
5. To describe the different types of enzyme inhibition. 1 1 58, 61
6. To describe the kinetics of an enzyme reaction 1 59, 60
7. To describe the different allosteric enzymes 1 1 62, 63
H. Amino Acids
1. To discuss the different properties of amino acids 1 34
2. To discuss the amino acid residues of albumin 1 37
3. To enumerate the different amino acids converted
to special products. 2 77, 78
4. To dicuss the different diseases associated with
amino acids deficiencies. 1 79
I. Nitrogen Metabolism
1. To discuss the different reactions involved in Urea 1 75
cycle.
2. To describe the different diseases assoociated with
urea cycle enzymes. 1 76
OBJECTIVES RECALL COMPRE- ANALYSIS/ QUESTION NO.
HENSION PROBLEM
SOLVING
J. Globular & Febrous proteins
1. To describe the unique structure of collagen. 1 32
2. To describe the structure of myoglobin 1 35
3. To describe the activiry of fibrous proteins. 1 52
K. Biochemistry of Hemoglobin
1. To describe the different hemoglobinopathies 1 2 55, 16, 54
2. To describe the structure hemoglobin in relation
to function.
3. To describe the process of binding of oxygen to 1 56
hemoglobin.
4. To describe the degradation of hemoglobin. 1 1 81, 82
L. Carbohydrate Structure and Function
1. To correlate the complex carbohydrates with their 1 4 61, 38, 39
respective diseases. 47, 48
M. Glycolysis & Gluconeogenesis
1. To describe the different enzymes involved in 2 44, 49
glycolysis.
2. To compare the process of glycolysis with that 1 45
of gluconeogenesis.
3. To describe the different rate limiting substrates in 1 46
glycolysis.
N. Lipid Structure & Function
1. To describe the structure and function of the dif- 1 84
ferent special lipids.
2. To discuss the activity of fatty acid synthase enzyme 1 98
O. Lipid Catabolism and B-oxidation
1. Explain the enzymatic breakdown of triacylglycerol 1 1 72, 99
2. Discuss the regulation of lipolysis. 1 73
P. Lipoprotein
1. To describe thestructures of the different lipoproteins 1 83
2. To discuss the drugs that control lipoprotein levels 1 100
in the blood.
Q. Cholesterol Metabolism
1. Discuss the different steps of cholesterol biosynthesis 1 85
R. Purine & Pyrimidine Biosynthesis
1. Discuss the different diseases associated with de- 1 1 95, 96
fective purine & pyrimidine biosynthesis.
2. Describe the de Novo purine biosynthesis 1 97
S. DNA Metabolism
1. To illustrate the structure of DNA 1 8
2. To describe the organization of the eukaryotic DNA 1 9
3. To discuss the intricacies of DNA synthesis 1 14
4. To compare DNA replication between prokaryotes
and the eukaryotes. 1 15
5. To describe the DNA promoter site. 1 23
T. RNA Metabolism
1. To describe the function of RNA Polymerase 1 21
2. To describe the process of intron splicing in mRNA
precursors. 1 22
3. To discuss the role of the sigma factor and the rho- 1 1 24, 28
factor in RNA synthesis.
4. To differentiate the RNA polymerases based on their 1 25
sensitivity to alpha-amaritin
5. To describe the structure & function of the different
RNA molecules. 2 1 26, 27, 30
OBJECTIVES RECALL COMPRE- ANALYSIS/ QUESTION NO.
HENSION PROBLEM
SOLVING
U. Protein Synthesis
1. To describe the genetic code 1 19
V. Mutation & Repair
1. Discuss the transfer of resistance in prokaryotes. 1 17
2. Discuss the UV damage to DNA 1 18
W. Recombinant DNA Technology
1. To discuss the different uses of recombinant DNA 2 1 10,13,20
techniques.
2. To define polymorphism. 1 11
3. To discuss the process of gene inactivation 1 12
X. Nutrition
1. Describe the different nutritional deficiencies in 2 4 87,88,91
humans 92,93,94
2. Discuss the different vitamins & nutrients & their 1 90
importance to nutrition.
Y. Biochemistry of the Immune System
1. To describe the structure of the different immuno-
globulin structures. 2 32,36