USER GUIDE

SMART PIPETTE - USER GUIDE

INTRODUCTION
Congratulations on making a SMART choice. You are now the proud
owner of an Accumax SMART pipette.

This user guide lists details on how you can take care of your SMART
pipette and benefit the most from its advanced features as follows:

• SMART is one of the most lightweight pipette with durable working.

• Designed to dispense accurate volume of liquid with long term
precision.
• Ergonomically designed for ease in operation and user safety.
• Color coded for easy identification.

CHECK LIST
Your Accumax SMART pack would carry...

• SMART Pipette
• Sample pack of tips
• Recalibration / Ellen Key
• Silicone Grease
• Shelf Mounting Stand
• User Guide
• Warranty Card
• Certificate of Conformity including Calibration Certificate

PRODUCT DESCRIPTION
SMART pipettes operate on air displacement principals and uses
disposable tips.

Variable volume pipette

CAT No. Volume Range [ µl ] Increments [ µl ]

SVA-125 0.1-2.5 0.002

SVA-100 0.5-10 0.02

SVA-200 5-50 0.1

SVA-500 10-100 0.1

SVA-600 100-1000 1

SVA-700 2-20 0.02

SVA-800 20-200 0.2

SVA-905 500-5000 10

SVA-1000 1000-10000 20

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 SMART PIPETTE - USER GUIDE

Fix Volume pipette

CAT No. Volume [ µl ]

SFA-205 2.5

SFA-5 5

SFA-10 10

SFA-20 20

SFA-25 25

SFA-50 50

SFA-100 100

SFA-200 200

SFA-250 250

SFA-500 500

SFA-1000 1000

SFA-2000 2000

SFA-5000 5000

SFA-10000 10000

Multichannel pipette

8-channels
CAT No. Volume Range [ µl ] Increments [ µl ]

SMA8-010 0.5 - 10 µl 0.02

SMA8-020 2- 20 µl 0.02

SMA8-050 5- 50 µl 0.1

SMA8-100 10 - 100 µl 0.1

SMA8-200 20 - 200 µl 0.2

SMA8-300 30 - 300 µl 0.2

12 channels
CAT No. Volume Range [ µl ] Increments [ µl ]

SMA12-010 0.5 - 10 µl 0.02

SMA8-020 2- 20 µl 0.02

SMA12-050 5 - 50 µl 0.1

SMA12-100 10 - 100 µl 0.1

SMA12-200 20 - 200 µl 0.2

SMA12-300 30 - 300 µl 0.2

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SMART PIPETTE - USER GUIDE

Digital Display

In SMART pipette, delivery volume is evidently indicated in the large

digital display on the handle.

In variable volume pipette, the last wheel of the counter has a

scale that assists to set precise delivery volume.

In some of the variable volume pipettes the last one or two counter
wheels will be in grey color which indicates the value after decimal.

A pointer is used to set exact or intermediate volumes with the help of

scale on last wheel.

Raw Material

The SMART pipette is made of mechanically robust and chemically

resistant materials, making it a durable and stable pipette giving
consisting performance for long time.

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 SMART PIPETTE - USER GUIDE

Parts Description

Aspiration / Dispensing Knob

(OPERATING KNOB)

Volume Changing Knob

Tip ejector pusher

COUNTER lock

Counter window cover

Counter Wheels

Main Handle COMFORT Grip

Main Handle

TIP EJECTOR Closer

Tip ejector

TIP cone

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SMART PIPETTE - USER GUIDE

PIPETTE OPERATION
Setting the Volume

With the SMART pipette, the delivery volume of liquid is set using the
digital counter. A pointer is used to set exact or intermediate volmes
using the scale on the last wheel of digital counter. In some of the
variable volume pipettes, counter wheels are in black & grey color.
Grey wheel indicates value after the decimal.

Locked Position Un-locked Position

Push up to un-lock Pull down to Lock

You can set the delivery volume

using the volume changing knob - +
on the upper side of pipette after
unlocking the counter by pulling
up the counter lock. To decrease
the delivery volume, turn it
clockwise and to increase, turn Volume
Changing
it anti-clockwise. Do make sure Knob
that the desired delivery volume is
set in-line with pointer. Pull down
the lock to ensure that volume
doesn’t accidently changed during
pipetting.

1) Counter lock mechanism is to ensure volume changing knob remains in its

position while aspirating or dispensing liquids. Any forced efforts to rotate the
volume changing knob while counter lock mechanism is in lock position will either
make it in unlock position or may damage the locking mechanism.

2) Setting the volume beyond the specified volume range is not advisable. Using
excessive force to turn the volume-changing knob outside the specified range,
may jam the mechanism and eventually damage the pipette.

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 SMART PIPETTE - USER GUIDE

Loading and Ejection Tips

Each SMART pipette is fitted with a tip ejector which helps eliminate
safety hazards associated with contamination.

It is necessary to check that the tip cone is clean before fitting a tip.
Press the tip on the cone of the pipette firmly to ensure an airtight
seal. The seal is set tight when a visible sealing ring forms between
the tip and the tip cone.

The tip ejector needs to be pressed downwards firmly with the thumb
to ensure proper tip ejection. Once the process is complete, make
sure that the tip is disposed of into a suitable waste container.

PIPETTing guidelines
• While using the pipette make sure that operating knob has been
handled slowly and smoothly.
• Ensure that the tip is firmly attached on the tip cone. Please check
for foreign particles and remove if any, around the tip cone.
• Make sure that the temperature of tip, pipette and liquid are at
equilibrium.
• While holding the pipette, its main handle should be resting on your
index finger.
• While aspirating, hold the pipette in upright position and keep the
tip at a constant depth below the surface of the liquid.
• You should pre-rinse the tip before aspirating the liquid by filling
and emptying the tip 5 times. This is important especially when
dispensing liquids, which have a viscosity and density different
from water.
• For the volatile solvents you should saturate the air-cushion of your
pipette by aspirating and dispensing the solvent repeatedly before
aspirating the sample.
• When pipetting liquids with temperatures different to the ambient
temperature, pre-rinse the tip several times before use.
• After pipetting acids or other corrosive liquids that emit vapors,
remove the tip-cone and rinse the piston, O-ring and seal with
distilled water.
• Do not pipette liquids having temperatures above 70°C or below
4°C.

Make sure that liquids never enter the tip-cone, to prevent this:

• Never lay the pipette on its side when there is liquid in the tip.
• Press and release the push knob slowly and smoothly.
• Never turn the pipette upside down

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SMART PIPETTE - USER GUIDE

PIPETTing TECHNIQUES
Forward Pipetting

1. Press the operating knob to the first stop.

2. Dip the tip under the surface of the liquid in the reservoir to a depth
of about 1cm and slowly release the operating knob. Withdraw the
tip from the liquid touching it against the edge of the reservoir to
remove excess liquid.
3. Dispense the liquid by gently 1 2 3 4
pressing the operating knob to
First Stop
the first stop. After a delay of Second Stop
about one second, continue to
press the operating knob all the
way to the second stop. This ac-
tion will empty the tip.
4. Release the operating button to
the ready position. Change the tip
and continue pipetting.

Reverse Pipetting

The reverse technique is suitable for dispensing liquids that have

a high viscosity or a tendency to foam easily. The technique is also
recommended for dispensing very small volumes.

1. Press the operating knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth
of about 1cm and slowly release the operating knob. This action
will fill the tip. Withdraw the tip from the liquid touching it against
the edge of the reservoir to
remove excess liquid.
3. Deliver the preset volume by 1 2 3 4
gently pressing the operating
First Stop
knob to the first stop. Hold the Second Stop

operating knob at the first stop.

Some liquid will remain in the tip
and it should not be dispensed.
4. The remaining liquid should
either be discarded with the tip
or should be dispensed back into
the reagent reservoir.

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 SMART PIPETTE - USER GUIDE

CALIBRATION AND ADJUSTMENT

All SMART pipettes are been quality tested according to ISO8655/DIN
12650. The quality control according to ISO 8655/DIN 12650 involves
gravimetric testing of each pipette with distilled water (quality 3, DIN
ISO3696) at 25°C +- 1° C.

All SMART pipettes are been calibrated in ISO/IEC 17025 accredited

laboratory. Each Pipette is calibrated, inspected and validated by
qualified technicians according to defined quality system.

SMART pipettes are constructed to permit re-adjustment for other

pipetting techniques or liquids of different temperature and viscosity.

Devise requirements and test conditions

An analytical balance must be used. The balance selection depends

upon selected model of the pipette and sensitivity of balance reading.

Test liquid: Water, distilled or deionized, grade 3 water conforming

ISO 3696.

Calibration should be carried out in a draft-free room at a constant

(+- 0.5°C) temperature of water, pipette and air between 15°C to 30°C.
The relative humidity must be above 50%, especially with volumes
under 50ul, the air humidly should be as high as possible to reduce
the effect of evaporation loss.

Special accessories for analytical balance, such as the evaporation

trap are recommended for the calibration of volumes under 50ul.

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SMART PIPETTE - USER GUIDE

Procedure to check calibration

The pipette is checked at maximum volume, at 50% of maximum

volume and at minimum or 10% of maximum volume, whichever is
higher.
• A new tip is first pre-wetted 3-5 times and a series of ten pipetting
is done at each volume.
• Use of forward pipetting technique is recommended.
• Calculate the inaccuracy and imprecision for all three volumes as
per EN ISO 8655/ DIN 12650 standards on the basis of the following
calculation

Conversion of weight readings to volume

_ _
Mean Value V = X · Z
_
Mean Value X = ∑ Xi
n
Xi = Balance Reading
n = number or reading
Z = Conversion factor
[example Z=1.0040 ul/mg at 25°c and 1013 hPa]

Calculation for in-accuracy (Systematic Error)

_
A% = V - Vo
· 100
Vo
_
V = Mean Value
Vo = Particular volume at which readings are taken

Calculation for Impression (Random Error)

n S = Standard Deviation
∑ ( Vi - V)2
S= i=1 V = Mean Value
n-1 n = number of readings

CV% = 100 · S
_
V

• Compare the results to the limits in the subsequent tables

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 SMART PIPETTE - USER GUIDE

Variable Volume Pipette

Incre- Test Inac- Impreci-
Color Volume Range
CAT No. ments Volume curacy sion
code [ µl ]
[ µl ] [µl] (±) % (±) %

0.25 12 6

SVA-125 0.1 - 2.5 µl 0.002 1.25 3 3

2.5 2.5 1.6

1 2.5 1.8

SVA-100 0.5-10 µl 0.02 5 2 1

10 1 0.6

5 2 2

SVA-200 5-50 µl 0.1 25 0.8 0.4

50 0.6 0.3

10 3 1

SVA-500 10-100 µl 0.1 50 1 0.5

100 0.8 0.2

100 3 0.6

SVA-600 100-1000 µl 0.1 500 1 0.4

1000 0.6 0.2

2 3 2

SVA-700 2-20 µl 0.02 10 1.5 1

20 0.9 0.4

20 2.5 0.7

SVA-800 20-200 µl 0.2 100 0.7 0.3

200 0.6 0.2

500 2.4 0.6

SVA-905 500-5000 µl 10 2500 1.2 0.25

5000 0.6 0.15

1000 3 0.6

SVA-1000 1000-10000 µl 20 5000 0.8 0.3

10000 0.6 0.2

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SMART PIPETTE - USER GUIDE

Fix Volume Pipette

Test
Color Volume Range Inaccuracy Imprecision
CAT No. Volume
code [ µl ] (±) % (±) %
[µl]

SFA-2.5 2.5 µl 2.5 2 1.6

SFA-0005 5 µl 5 1.3 1.2

SFA-0010 10 µl 10 1.2 0.8

SFA-0020 20 µl 20 1 0.5

SFA-0025 25 µl 25 1 0.3

SFA-0050 50 µl 50 0.7 0.3

SFA-0100 100 µl 100 0.6 0.2

SFA-0200 200 µl 200 0.6 0.2

SFA-0250 250 µl 250 0.6 0.2

SFA-0500 500 µl 500 0.6 0.2

SFA-1000 1000 µl 1000 0.6 0.2

SFA-2000 2000 µl 2000 0.3 0.15

SFA-5000 5000 µl 5000 0.3 0.15

SFA-10000 10000 µl 10000 0.6 0.2

Multi Channel Pipette

8 Channels & 12 Channels
Test
Volume Incre- Inac- Impreci-
Color Vol-
CAT No. Range ments curacy sion
code ume
[ µl ] [ µl ] (±) % (±) %
[µl]

1 8 5
SMA8-010
SMA12-010 0.5-10 µl 0.02 5 4 2

10 2 1

2 7 3
SMA8-020
2-20 µl 0.02 10 3 2
SMA12-020
20 2 1.6

5 3 2
SMA8-050
5-50 µl 0.1 25 1.5 1
SMA12-050
50 1 0.7

10 3 2
SMA8-100 10 – 100
0.1 50 1 0.8
SMA12-100 µl
100 0.8 0.3

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 SMART PIPETTE - USER GUIDE

20 5 1.4
SMA8-200 20-200
0.2 100 1 0.4
SMA12-200 µl
200 0.7 0.25

30 3 1
SMA8-300 30-300
0.2 150 1 0.5
SMA12-300 µl
300 0.6 0.3

Calibration Adjustment

1. Calibration adjustment is done with the Recalibration (Ellen) key.

2. Remove the calibration guard and place the Recalibration (Ellen)
key into the opening of the calibration screw on the side of the
handle.
3. Turn the key clockwise to increase or counterclockwise to de-
crease the volume.
4. After adjustment, check the calibration according to the instruc-
tions above.
5. Once within permissible error range, place calibration guard in its
position to avoid accidental changes in calibration.

For more detail information about Calibration and Adjustment, visit

www.accumaximum.com/calibration

Depending upon use, we recommend checking of calibration every six months.

However this can be adjusted to individual requirements.

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SMART PIPETTE - USER GUIDE

MAINTENANCE
When the SMART pipette is not in use, please make sure that it is
stored in an upright position. Accumax recommends the SMART
stand for this purpose.

You should check the pipette at the beginning of each day for accu-
mulation of dust and dirt on the outside surfaces. Attention should
be paid to the tip cone. No other solvents except Iso-propanol should
be used to clean the pipette. If the pipette is used daily, it should be
inspected every three months.

The servicing procedure starts with the disassembly of the pipette.

Please refer to the spare parts lists for better understanding of the
components. Shown here is an example of dis-assembly and as-
sembly procedure of 100-1000 μl pipette, the basic procedure is to be
followed for all other volume pipettes.

1 2 3

1. For opening press 2. Rotate the tip ejector 3. Turn out the tip ejector,
the tip ejector closer closer in the direction slowly
upwards indicated until the
snap

• Pull out the piston and other parts by unscrewing the tip cone.
Push out with piston the rest of the piston assembly including the
Seal and O-ring. Remember to keep all parts in order for reassem-
bly.
• Clean the piston, the piston spring, Seal and the O-ring with a iso-
propanol and lint free tissue. Allow them to dry.
• Check the tip cone for foreign particles and remove, if any.
• Grease the cleaned parts with the approved lubricant provided with
each pipette.
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 SMART PIPETTE - USER GUIDE

1
Align the notch on the tip ejector with the slots on tip
ejector closer as indicated

2
Ensure the notch on the main handle matches the
slots on the tip ejector closer. Push the tip ejector
closer upwards as indicated.

3
Insert both parts in the aligned manner as indicated.

Once completely closed, twist the tip ejector closer in

the indicated direction to close

For correct re-assembly, it is crucial that the slots on the tip ejector closer match
the notches on the inside of the main handle. Please follow the above procedure.

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SMART PIPETTE - USER GUIDE

List of Spare Parts

Group 1

Variable Volume 100-100µl

Fix Volume 250µl

500µl
1000µl

SP 377-1

SP 302-1

SP 70-1
SP 23-1

SP 317-1

SP 392-1

SP 259-1

SP 44-1 SP 314-1

SP 14-1

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 SMART PIPETTE - USER GUIDE

Group 2 Group 3

Variable Volume 0.1-2.5µl Variable Volume 0.5-10µl

Fix Volume 0.5µl Fix Volume 10µl

2.5µl

SP 41-1 SP 41-2

SP 301-1

SP 301-1

SP 48-2 SP 48-2

SP 258-1

SP 48-1 SP 259-1

SP 48-1
SP 38-1

SP 38-1

SP 25-1

SP 25-1

SP 314-1

SP 314-1
SP 14-1

SP 14-1

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SMART PIPETTE - USER GUIDE

Group 4 Group 5
Variable Volume 2-20µl Variable Volume 5-50µl

Fix Volume 20µl Fix Volume 25µl

50µl

SP 39-3
SP 40-1

SP 301-1
SP 306-1

SP 48-2 SP 45-1

SP 391-1
SP 265-1

SP 260-1
SP 47-1
SP 48-1

SP 38-1
SP 38-1

SP 25-1

SP 24-1

SP 314-1
SP 314-1

SP 14-1
SP 14-1

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 SMART PIPETTE - USER GUIDE

Group 6 Group 7
Variable Volume 10-100µl Variable Volume 20-200µl

Fix Volume 100µl Fix Volume 200µl

SP 39-1 SP 366-1

SP 303-1
SP 305-1

SP 45-1
SP 45-1

SP 386-1
SP 261-1 SP 388-1
SP 264-1

SP 46-1
SP 45-1

SP 38-1 SP 38-1

SP 24-1 SP 24-1

SP 314-1 SP 314-1

SP 14-1
SP 14-1

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SMART PIPETTE - USER GUIDE

Group 8 Group 9

Variable Volume 1-5ml Variable Volume 1-10ml

Fix Volume 2ml Fix Volume 10ml

5ml

SP 31-2 SP 30-2

SP 51-1 SP 50-1

SP 53-1
SP 52-1

SP 353-1 SP 353-1
SP 304-1 SP 304-2
SP 57-2 SP 57-1
SP 263-1 SP 262-1
SP 42-1 SP 43-1

SP 28-1
SP 29-1

SP 26-1
SP 27-1

SP 312-1
SP 313-1
SP 55-1
SP 54-1

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 SMART PIPETTE - USER GUIDE

autoclaving
Accumax SMART pipette is completely autoclavable at 2 bar pres-
sure, 121°c temperature for 20 minutes exposure time according to
DIN.

• Keep digital counter in unlock position.

• Autoclave the complete pipette without any disassembling.
• After autoclaving, allow pipette to completely cool and dry.

If the pipette is autoclaved frequently, the piston and springs should

be greased with the lubricant supplied with each pipette to maintain
smooth movement.

TROUBLE SHOOTING GUIDE

Problem area Possible Cause Solution

Pipette is Worn O-ring or seal Replace worn parts
leaking
Foreign particles between tip Clean tip cone, attach
and tip cone new tip
Foreign particles between Clean seal and piston
piston and seal
Pipette wont Worn O-ring or seal Replace worn parts
aspirate
Tip cone is loose Tighten tip cone
Piston is damaged Return pipette to
(Chemically or Mechanically) Authorised Distributor
Damaged tip cone Replace the tip cone
Pipette is Improper assembly See “Maintenance”
inaccurate section
Tip cone is loose Tighten tip cone
Tip incorrectly attached Attach firmly
Calibration altered Recalibrate according to
instructions
Inaccurate Calibration not suitable for Recalibrate with the
dispensing with particular liquid liquid in question
certain liquids

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