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Q3 - WEEK 1 - LAS-1-Process-involved-in-Genetic-Engineering

The document outlines the basic steps of genetic engineering which are: 1) isolating DNA fragments from donor organisms, 2) inserting the isolated DNA into a vector, and 3) growing the recombinant vector in a host. It provides examples of applying each step, such as isolating the human insulin gene and inserting it into a vector. Students are asked to match genetic engineering steps to processes and answer questions about the importance and morality of cloning.

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Bernard D. Fajardo Jr.
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400 views2 pages

Q3 - WEEK 1 - LAS-1-Process-involved-in-Genetic-Engineering

The document outlines the basic steps of genetic engineering which are: 1) isolating DNA fragments from donor organisms, 2) inserting the isolated DNA into a vector, and 3) growing the recombinant vector in a host. It provides examples of applying each step, such as isolating the human insulin gene and inserting it into a vector. Students are asked to match genetic engineering steps to processes and answer questions about the importance and morality of cloning.

Uploaded by

Bernard D. Fajardo Jr.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Name: Grade & Section: 11 Score: School:

Teacher: Subject: Gen. Bio 2

Lesson Topic: Processed Involved in Genetic Engineering Quarter 3, Week 1, LAS


Learning Targets: Outline the process involved in genetic Engineering.
Reference(s): Tecson- Mendoza EM (Ed.) 2014, Understanding Biotechnology. Second Edition, National Academy of Science
and Technology- Philippines Science Heritage Foundation Incorporated, Manila Philippines pp.17-38.

Processed Involved in Genetic Engineering


Genetic Engineering is the process of making changes on the genetic code of an organism using recombinant DNA (rDNA)
technology to alter the genetic make up of an organism. Genetic engineering involves the direct manipulation of one or more genes. Most often a
gene from another species is added to an organism genome to give it desired phenotype.

Basic Steps of Genetic Engineering:


1. The isolation of DNA fragments from a donor organism.
To Isolate a specific gene, one often begins by constructing a DNA library – a comprehensive collections of cloned DNA fragments
from a cell , tissue, or organism. A virus or plasmid used in this way is known as a cloning vector, and the DNA propagated by insertion
into it is said to have been cloned.

Example: The nucleus and the cell are broken open, thus releasing of DNA

2. The insertion of isolated donor DNA fragments into Vector genome.


In a typical DNA cloning procedure, the genes or other DNA fragment of interest is first inserted into a circular piece of DNA called a
plasmid. As they reproduce, they replicate the plasmid and pass it on to their offspring, making copies of the DNA it contain.

Example: The human insulin is inserted into vector.

3. The growth of recombinant vector in an appropriate host.


The ligated recombinant DNA enters bacterial cell by transformation. After it is in the host cell, the plasmid vector is able to replicate
because plasmids normally have a replication origin.

Example: DNA cloning

Activity:
A. Identify the basic Step of Genetic engineering applied to each situation. Write the letter of your answer on the space provided.
a. The Isolation of DNA fragments from donor organisms.
b. The insertion of Isolated donor DNA fragments into Vector genome.
c. The growth of recombinant vector in an appropriate host.
1. DNA introduces into bacterial cells.
2. DNA ligase added seals overhang.
3. Recombinant DNA molecules replicate and cell divide.
4. Shoot and roots regeneration.
5. Cells are treated with polyethylene glycol to create random recombination.
6. Extraction of gene of interest from the donor organisms.
7. The human Insulin genes is inserted into vector.
8. The recombinant plasmid is introduced into bacterial cell.
9. Human insulin is isolated from human DNA.
10. Cloning of DNA or genes.

B. Explain each question briefly. Write your answer at the back portion of the paper.
1. What is the importance of DNA cloning?
2. Is cloning morally right? Why or Why not?

NOTE: Please copy and paste the link down below on your internet browser for more references .
http://bitly.ws/ckL2
http://bitly.ws/ckLa

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