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PMLS 1: Lab Math Ratios and Dilutions

This document provides examples of calculating ratios and dilutions for solutions in medical laboratory science. It addresses topics like determining the ratio and dilution when blood and saline are mixed, calculating volumes of blood and anticoagulant for a given ratio, and preparing solutions of specific concentrations by diluting stock solutions. For example, it shows how to prepare 1 liter of a 1:100 dilution of sodium hypochlorite by adding 0.01 L of bleach to 0.99 L of water.

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AEsmiling
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344 views2 pages

PMLS 1: Lab Math Ratios and Dilutions

This document provides examples of calculating ratios and dilutions for solutions in medical laboratory science. It addresses topics like determining the ratio and dilution when blood and saline are mixed, calculating volumes of blood and anticoagulant for a given ratio, and preparing solutions of specific concentrations by diluting stock solutions. For example, it shows how to prepare 1 liter of a 1:100 dilution of sodium hypochlorite by adding 0.01 L of bleach to 0.99 L of water.

Uploaded by

AEsmiling
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Principles of MLS Practice 1

Ratios & Dilutions

NAME: AEsmiling Date: Aug

1. Twenty microliters of blood was mixed with 100 microliters of normal saline solution.
a. What is the ratio of blood to normal saline? 1:5
b. What is the dilution of the solution? 1/6

2. A 5 mL blood has a 9:1 blood to anticoagulant ratio. What are the volume of blood and the volume of anticoagulant in
this specimen?

5mL/9=x; x= 0.56 mL;


there is 5 mL of blood to 0.56 mL anticoagulant.
(when mixed, to a total of 5.56 mL. solution)

3. A 1:5 dilution of reagent A to distilled water is required. If exactly 50 microliters of reagent A should be diluted, how
much distilled should be added to reagent A to achieve this dilution?

1/5 = 50 microliters/x ; x = 250 microliters


250 microliters - 50 microliters reagent A = 200 microliters diluent;
therefore, 200 microliters diluent is required of distilled water

4. How would you prepare 1 liter of 1:100 dilution of sodium hypochlorite solution?
Add 0.01 L of bleach to 0.99 L of clean water.

5. How would you prepare 100 mL of 0.1 M HCl using 1 M Hcl?


M = moles/liters ; 100 mL = 0.1 L
M1 = 1 M Hcl, M2 = 0.1 M Hcl, V2 = 0.1 L , V1 = ?
V1 = [(0.1 M)(0.1 L)]/1 M Hcl = 0.01 L
0.01 L = 10 mL
Take 10 mL of 0.1 M Hcl and dilute it to 100 mL

6. How would you prepare 500 mL of 0.85% saline (NaCl) solution:

0.85% = 0.85 g/100 mL H2O; normal saline solution


0.85 g/100 mL H2O = x / 500 mL ;
x = 4.25 g of sodium chloride
is needed to mix in 500 mL water.

7. A solution with 100 g/mL of glucose was diluted 1:10. What is the concentration of glucose after dilution?
M1 = 100 M ; V1 = 0.1 mL ; V2 = 1 mL ; M2 = [(100 M)(0.1 mL)] 1 mL = 10 g/mL
or 1/10 x 100g/mL = 10 g/mL

8. A serum sample was diluted 1:5 then diluted further 1:8. What is the final dilution of serum?

1/5 x 1/8 = 1/40 or 1:40


9. Four tubes were prepared. Two milliliters of serum was added to the first tube and 3 mL of distilled water was added
to tubes 2-4. One milliliter was transferred from tube one to tube two and mixed. One milliliter was transferred from
tube two to tube three and mixed. One milliliter was transferred from tube 3 to tube 4. One milliliter from tube 4 was
discarded. What is the dilution of tubes 2-4?

1/4 x 1/4 x 1/4 = 1/64 or 1:64

10. Based on question number 9, if the concentration of anti-HBs antibodies in tube 4 was 100 IU/mL, what would be the
reported concentration of anti-Hbs?

(2nd tube) 1 / 4
(3rd tube) 1 / 4 x 1 / 4 = 1 / 16
(4th tube) 1 / 4 x 1 / 4 x 1 / 4 = 1 / 64
64(100 IU/mL)= 16x
x = 400 IU/mL, for 3rd tube
64(100 IU/mL) = 4x
x = 1600 IU/mL, for 2nd tube

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