HR2000+ Spectrometer

Installation and Operation Manual

For Products: HR2000+, HR2000+CG, HR2000+ES

Document: 294-00000-000-02-201502

000-00000-000-02-A
AMERICAS & WORLD HEADQUARTERS

Phone: +1 727-733-2447
Ocean Optics, Inc.
Fax: +1 727-733-3962
830 Douglas Ave.
Dunedin, FL 34698
Sales: info@oceanoptics.com USA
Orders: orders@oceanoptics.com
Support: techsupport@oceanoptics.com Manufacturing & Logistics
4301 Metric Dr.
Winter Park, FL 32792
USA

EUROPE, MIDDLE EAST & AFRICA

Phone: +31 26-319-0500 Sales & Support
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73760 Ostfildern
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ASIA
Phone: +86 21-6295-6600 Ocean Optics Asia
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Email: asiasales@oceanoptics.com Kirin Tower Suite 601B
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Japan & Korea: +82 10-8514-3797 Shanghai
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www.oceanoptics.com

Copyright © 2010 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical,
photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
Trademarks
All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.
Limit of Liability
Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is
on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from
the information contained in this manual.
 Table of Contents
About This Manual......................................................................................................... iii
Document Purpose and Intended Audience.............................................................................. iii
What’s New in this Document ................................................................................................... iii
Document Summary .................................................................................................................. iii
Product-Related Documentation .................................................................................... iii
Upgrades ....................................................................................................................... iv
Warranty ........................................................................................................................ iv

Chapter 1: Introduction ..................................................................... 1

Product Overview .......................................................................................................... 1
System Requirements ................................................................................................... 2
EEPROM Utilization .................................................................................................................. 2
About OceanView Software....................................................................................................... 2
Sampling System Overview....................................................................................................... 2
How Sampling Works ............................................................................................................ 2
Modular Sampling Accessories ............................................................................................. 3
Interface Options ........................................................................................................... 3
Breakout Box ............................................................................................................................. 3
Shipment Components .................................................................................................. 3

Chapter 2: Installing the HR2000+ .................................................... 5

Overview ....................................................................................................................... 5
HR2000+ Installation ..................................................................................................... 5

Chapter 3: Troubleshooting .............................................................. 7

Overview ....................................................................................................................... 7
HR2000+ Connected to PC Prior to Software Installation .............................................. 7
Windows Operating Systems .................................................................................................... 7
Remove the Unknown Device from Windows Device Manager ........................................... 7
Remove Improperly Installed Files ........................................................................................ 8
Mac Operating Systems ............................................................................................................ 8
Linux Operating Systems .......................................................................................................... 9

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Table of Contents

Appendix A: Calibrating the Wavelength of the HR2000+ .............. 11

Overview ....................................................................................................................... 11
About Wavelength Calibration ....................................................................................... 11
Calibrating the Spectrometer ......................................................................................... 12
Preparing for Calibration............................................................................................................ 12
Calibrating the Wavelength of the Spectrometer ...................................................................... 12
Saving the New Calibration Coefficients: USB Mode ..................................................... 14

Appendix B: Specifications ............................................................... 15

Overview ....................................................................................................................... 15
How the HR2000+ Works .............................................................................................. 15
HR2000+ Components Table .................................................................................................... 16
HR2000+ Specifications ................................................................................................ 17
CCD Detector Specifications ..................................................................................................... 17
HR2000+ Spectrometer ............................................................................................................. 17
System Compatibility ..................................................................................................... 18
Compatibility for Desktop or Notebook PCs .............................................................................. 18
30-Pin Accessory Connector Pinout .............................................................................. 19
30-Pin Accessory Connector Pinout Diagram ........................................................................... 19
30-Pin Accessory Connector – Pin Definitions and Descriptions .............................................. 19
30-Pin J2 Accessory Connector - Part Numbers....................................................................... 21
HR2000+ 15-Pin Accessory Cable Pinout ..................................................................... 22

Index ................................................................................................... 23

ii 294-00000-000-02-201502
About This Manual
Document Purpose and Intended Audience
This document provides the user of HR2000+ Spectrometer with instructions for setting up, calibrating
and performing experiments with their spectrometer.

What’s New in this Document

This version of the HR2000+ High-speed Fiber Optic Spectrometer, Installation and Operation Manual
updates information for OceanView.

Document Summary
Chapter Description

Chapter 1: Introduction Contains descriptive information about the HR2000+

Spectrometer and how sampling works. It also provides a list
of system requirements, interface options, and shipment
components.

Chapter 2: Installing the HR2000+ Provides installation and configuration instructions.

Chapter 3: Troubleshooting Contains recommended steps to isolate and correct common

problems.

Appendix A: Calibrating the Provides instructions for calibrating the HR2000+

Wavelength of the HR2000+ Spectrometers.

Appendix B: Specifications Contains technical specifications and connector pinouts for the
HR2000+ Spectrometers.

Product-Related Documentation
You can access documentation for Ocean Optics products by visiting our website at
http://www.oceanoptics.com. Select Technical Operating Instructions, then choose the appropriate
document from the available drop-down lists.

Document for… Document Location

OceanView software http://oceanoptics.com///wp-content/uploads/OceanViewIO.pdf

SpectraSuite software http://oceanoptics.com///wp-content/uploads/SpectraSuite.pdf

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About This Manual

Document for… Document Location

HR-4 Breakout Box http://oceanoptics.com///wp-content/uploads/HR-4-Breakout-Box.pdf

External triggering for http://oceanoptics.com///wp-content/uploads/External-Triggering-Options.pdf

firmware versions
below 3.0

External triggering for http://oceanoptics.com///wp-content/uploads/External-Triggering-

firmware versions 3.0 Options_Firmware3.0andAbove.pdf
and above

Ocean Optics offers a Glossary of spectroscopy terms to help you further understand your state-
of-the-art products and how they function, located at: http://oceanoptics.com/glossary/.

Upgrades
Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

Warranty
Our 3-Year Warranty covers Ocean Optics miniature fiber optic spectrometers, light sources and
sampling accessories – regardless of the application – from manufacturing defects. It also covers fibers
and probes for a full 12 months: http://oceanoptics.com/services/exclusive-3-year-warranty/.
This comprehensive warranty ensures you of the highest level of craftsmanship and reliability for years to
come. No other manufacturer offers such a solid guarantee of quality and reliability.

The Ocean Optics 3-Year Warranty applies to Ocean Optics equipment (excluding OEM configurations)
purchased on or after July 1, 2010. The warranty covers parts and labor needed to repair manufacturing
defects that occur during the warranty period. We also will cover the costs of shipping warranty-related
repairs from our customers to Ocean Optics and from us to our customers.

ISO Certification
Ocean Optics, the industry leader in miniature photonics, has been certified for ISO 9001:2008
certification applicable to the design and manufacture of electro-optical equipment since 2009.

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 About This Manual

WARNING

This is a Class A product. In a domestic environment this product may cause radio
interference in which case the user may be required to take adequate measures.

FCC COMPLIANCE

This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses and can
radiate radio frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful
interference in which the user will be required to correct the interference at his own
expense.

WARNING: The authority to operate this equipment is conditioned by the

requirement that no modifications will be made to the equipment unless the changes
or modifications are expressly approved by the manufacturer.

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About This Manual

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 Chapter 1

Introduction
Product Overview
The HR2000+ High-Speed Miniature Fiber Optic Spectrometer provides optical resolution as good as
0.035 nm (FWHM). The HR2000+ is responsive from 200-1100 nm, but the specific range and resolution
depends on your grating and entrance slit selections. With its capability of transferring 1ms spectra
continuously, the HR2000+ is the fastest spectrometer available from Ocean Optics.
The HR2000+ is perfect for applications where fast reactions need to be monitored and high resolution is
necessary, such as chemistry and biochemistry applications.
Data programmed into a memory chip on each HR2000+ includes wavelength calibration coefficients,
linearity coefficients, and the serial number unique to each spectrometer. Our spectrometer operating
software simply reads these values from the spectrometer — a feature that enables hot swapping of
spectrometers among PCs.
The HR2000+ Spectrometer connects to a notebook or desktop PC via USB port or serial port. When
connected to the USB port of a PC, the HR2000+ draws power from the host PC, eliminating the need for
an external power supply. The HR2000+, like all our USB devices, can be controlled by our OceanView
software, a Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux
operating systems.

Ocean Optics HR2000+ High-speed Fiber Optic Spectrometer

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1: Introduction

System Requirements
You can use the HR2000+’s USB connectivity with any computer that meets the following requirements:
• Microsoft Windows – Windows 2000/XP/7; 32-bit and 64-bit and Windows Vista (32-bit only)
• Apple Macintosh – version 10.5 Intel or later
• Linux – Any version released for an x86 or amd64 platform since 2010

Alternately, the HR2000+ has serial port adaptability for connecting to PCs, PLCs, and other devices that
support the RS-232 communication protocol. However, this connection method requires an external
power supply to power the HR2000+, the Breakout Box (HR4-BREAKOUT), and a serial cable.

EEPROM Utilization
An EEPROM memory chip in each HR2000+ contains wavelength calibration coefficients, linearity
coefficients, and a serial number unique to each individual spectrometer. The software application reads
these values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs
without entering the spectrometer coefficients manually on each PC.

About OceanView Software

OceanView is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device.
OceanView is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With OceanView, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the OceanView manual for hardware requirements when using OceanView (see
Product-Related Documentation).

Sampling System Overview

How Sampling Works
Ocean Optics components function in a sampling system as follows:
1. The user stores reference and dark measurements to correct for instrument response variables.
2. The light transmits through an optical fiber to the sample.
3. The light interacts with the sample.
4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.
5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.

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 1: Introduction

6. The spectrometer passes the sample information to the spectrometer operating software.
7. The software compares the sample to the reference measurement and displays processed spectral
information.

Modular Sampling Accessories

Ocean Optics offers a complete line of spectroscopic accessories for use with the HR2000+. Most of our
spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the
sampling system components is as easy as unscrewing a connector and replacing an accessory.

Interface Options
The HR2000+ has both USB and serial port connectors (with the use of an adapter), enabling you to
connect the spectrometer to a desktop or notebook PC via a USB port. However, you must create custom
software if using the serial port. OceanView software is available if you are connecting via the USB port.

Breakout Box
Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the
signals from their 30-pin port to an array of standard connectors and headers, enabling easy access to a
variety of features found in Ocean Optics’ HR2000+ Spectrometer. In addition to the accessory
connector, the breakout box features a circuit board based on a neutral breadboard pattern that allows
custom circuitry to be prototyped on the board itself.

Shipment Components
The following information and documentation ships with the HR2000+ Spectrometer:
 Packing List
The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.
 USB Cable (USB-CBL-1)
Use this cable to connect your spectrometer to a computer running on a Windows, Mac or Linux
operating system.
 Wavelength Calibration Data Sheet
Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. OceanView reads this calibration data from your spectrometer when
it interfaces to a computer via the USB port.

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1: Introduction

Note

Please save the Wavelength Calibration Data Sheet for future reference.

Other Accessories Available

Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy
needs.
 Fibers
 Light Sources
 Integrated Sampling Systems
 Cuvettes
 Filter Holders
 Lithium Ion Battery Pack
 HR4-BREAKOUT Breakout Box

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 Chapter 2

Installing the HR2000+

Overview
You must install the operating software application prior to connecting the HR2000+ Spectrometer to the
PC. The software installation installs the drivers required for HR2000+ installation. If you do not install
the software first, the system will not properly recognize the HR2000+.
If you have already connected the HR2000+ to the PC prior to installing the software, consult Chapter 3:
Troubleshooting for information on correcting a corrupt HR2000+ installation.

HR2000+ Installation
USB Mode
Note

The USB port on a computer can power up to five HR2000+ spectrometer channels.
Systems with more than five channels require a powered USB hub.

► Procedure
Follow the steps below to connect the HR2000+ to a computer via the USB port:
1. Install the spectrometer operating software on the destination computer.
2. Locate the USB cable (USB-CBL-1) provided with the HR2000+.
3. Insert the square end of the cable into the side of the HR2000+.
4. Insert the rectangular end of the cable into the USB port of the computer.
If you installed the spectrometer operating software prior to connecting the HR2000+, the software
installs the HR2000+ drivers. If the drivers do not successfully install (or if you connected the HR2000+
to the computer before installing the software), consult Chapter 3: Troubleshooting.
If you have followed the previous steps and started the software, the spectrometer is already acquiring
data.
Once you install the software and hardware, and establish your sampling system, you are ready to take
measurements.

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2: Installing the HR2000+

Serial Port Mode

To use the serial port capacity of the HR2000+ Spectrometer, the computer must be running a 32-bit
version of the Windows operating system.

► Procedure
Follow the steps below to connect the HR2000+ to the computer via serial port:
1. Connect the serial cable adapter block to the appropriate pins of the HR2000+’s 30-Pin
Accessory Connector.
2. Connect one end of the 9-pin serial cable to the adapter block on the HR2000+, and then connect
the other end to a serial port on the computer.
3. Note the number of the serial port (COM Port) to which you connected the HR2000+ (some
computers may not have numbered ports; handheld computers typically have only one serial
port).
4. Plug the 5 VDC external power supply into an outlet and connect it to the HR2000+.

Connect Spectroscopic Accessories

To find operating instructions for HR2000+-compatible products (such as light sources, sampling
chambers, and probes), consult the Ocean Optics website at http://oceanoptics.com/support/technical-
documents/.

HR2000+ Configuration
The HR2000+ can be used with OceanView operating software when connected to the USB port.
If you have followed the previous steps and started OceanView, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

External Triggering Options

You can trigger the HR2000+ using a variety of External Triggering options through the 30-pin
Accessory Connector on the spectrometer. For HR2000+ firmware versions below 3.0, see the
External Triggering Options document located at http://oceanoptics.com///wp-
content/uploads/External-Triggering-Options.pdf. For HR2000+ firmware version 3.0 and above, see the
External Triggering Options document located at http://oceanoptics.com///wp-content/uploads/External-
Triggering-Options_Firmware3.0andAbove.pdf. The triggering document contains instructions for
configuring External Triggering options for the HR2000+.

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 Chapter 3

Troubleshooting
Overview
The following sections contain information on troubleshooting issues you may encounter when using the
HR2000+ Spectrometer.

HR2000+ Connected to PC Prior to Software

Installation
Windows Operating Systems
If you connected your Ocean Optics USB device to the computer prior to installing your Ocean Optics
software application, you may encounter installation issues that you must correct before your Ocean
Optics device will operate properly.
Follow the applicable steps below to remove the incorrectly installed device, device driver, and
installation files.

Note

If these procedures do not correct your device driver problem, you must obtain the
Correcting Device Driver Issues document from the Ocean Optics website:
http://oceanoptics.com///wp-content/uploads/Correcting-Device-Driver-Issues.pdf

Remove the Unknown Device from Windows Device Manager

► Procedure
1. Open Windows Device Manager. Consult the Windows operating instructions for your computer
for directions, if needed.
2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+"
sign to the immediate left.

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3: Troubleshooting

Note

Improperly installed USB devices can also appear under the Universal Serial Bus
Controller option. Be sure to check this location if you cannot locate the unknown device.

3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown
Device listing and select the Uninstall or Remove option.
4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown
Device. Click the OK button to confirm the device removal.
5. Disconnect the HR2000+ from your computer.
6. Locate the section in this chapter that is appropriate to your operating system and perform the
steps in the following Remove Improperly Installed Files section.

Remove Improperly Installed Files

► Procedure
1. Open Windows Explorer.
2. Navigate to the Windows | INF directory.

Note

If the INF directory is not visible, you must disable the Hide System Files and Folders
and Hide File Extensions for Known File Types options in Windows Folder Options.
Access Windows Folder Options from Windows Explorer, under the Tools | Folder
Options menu selection.

3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows
2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory.
4. Navigate to the Windows | System32 | Drivers directory.
5. Delete the EZUSB.SYS file.
6. Reinstall your Ocean Optics application and reboot the system when prompted.
7. Plug in the USB device.
The system is now able to locate and install the correct drivers for the USB device.

Mac Operating Systems

Since there are no device files for the HR2000+ Spectrometer in a Mac operating system, you should not
encounter any problems if you installed the spectrometer before the spectrometer operating software.

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 3: Troubleshooting

Linux Operating Systems

For Linux operating systems, all you need to do is install the spectrometer operating software, then
unplug and replug in the spectrometer. Technically, the driver files for Linux simply give nonprivileged
users permission to use newly connected hardware. There isn’t any long-term harm to plugging in the
device before installing the software.

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3: Troubleshooting

10 294-00000-000-02-201502
 Appendix A

Calibrating the Wavelength of

the HR2000+
Overview
This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
HR2000+.

About Wavelength Calibration

You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:

λp = I + C1 p + C2 p2 + C3 p3

Where:
λ = the wavelength of pixel p
I = the wavelength of pixel 0
C1 = the first coefficient (nm/pixel)
C2 = the second coefficient (nm/pixel2)
C3 = the third coefficient (nm/pixel3)
Rλ = the reference intensity at wavelength λ

You will be calculating the value for I and the three Cs.

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A: Calibrating the Wavelength of the HR2000+

Calibrating the Spectrometer

Preparing for Calibration
To recalibrate the wavelength of your spectrometer, you need the following components:
• A light source capable of producing spectral lines

Note

Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an
HG-1, you need a light source that produces several (at least 4-6) spectral lines in the
wavelength region of your spectrometer.

• An HR2000+ spectrometer
• An optical fiber (for spectrometers without a built-in slit, a 50-µm fiber works best)
• A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-
order linear regressions

Note

If you are using Microsoft Excel, choose Tools | Add-Ins and check AnalysisToolPak
and AnalysisTookPak-VBA.

Calibrating the Wavelength of the Spectrometer

► Procedure
Perform the steps below to calibrate the wavelength of the spectrometer:
1. Place the software into Quick View (Scope) mode and take a spectrum of your light source.
Adjust the integration time (or the A/D conversion frequency) until there are several peaks on the
screen that are not off-scale.
2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum
intensity.
3. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.
4. Use the spreadsheet program or calculator to create a table like the one shown in the following
figure. In the first column, place the exact or true wavelength of the spectral lines that you used.
In the second column of this worksheet, place the observed pixel number. In the third column,
calculate the pixel number squared, and in the fourth column, calculate the pixel number cubed.

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 A: Calibrating the Wavelength of the HR2000+

Independent Values Computed

Dependent from the Regression
Variable Variables Output

Predicted
True Wavelength (nm) Pixel # Pixel # 2 Pixel # 3 Difference
Wavelength
253.65 175 30625 5359375 253.56 0.09
296.73 296 87616 25934336 296.72 0.01
302.15 312 97344 30371328 302.40 -0.25
313.16 342 116964 40001688 313.02 0.13
334.15 402 161604 64964808 334.19 -0.05
365.02 490 240100 117649000 365.05 -0.04
404.66 604 364816 220348864 404.67 -0.01
407.78 613 375769 230346397 407.78 0.00
435.84 694 481636 334255384 435.65 0.19
546.07 1022 1044484 1067462648 546.13 -0.06
576.96 1116 1245456 1389928896 577.05 -0.09
579.07 1122 1258884 1412467848 579.01 0.06
696.54 1491 2223081 3314613771 696.70 -0.15
706.72 1523 2319529 3532642667 706.62 0.10
727.29 1590 2528100 4019679000 727.24 0.06
738.40 1627 2647129 4306878883 738.53 -0.13
751.47 1669 2785561 4649101309 751.27 0.19

5. Use the spreadsheet or calculator to calculate the wavelength calibration coefficients. In the
spreadsheet program, find the functions to perform linear regressions.
• If using Quattro Pro, look under Tools | Advanced Math
• If using Excel, look under Analysis ToolPak
6. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number
squared, and the pixel number cubed as the independent variables (X). After executing the
regression, you will obtain an output similar to the one shown below. Numbers of importance are
noted.
Regression Statistics
Multiple R 0.999999831
R Square 0.999999663 R Squared
Adjusted R Square 0.999999607
Standard Error 0.125540214
Observations 22

Intercept

Coefficients Standard Error

Intercept 190.473993 0.369047536 First coefficient
X Variable 1 0.36263983 0.001684745
X Variable 2-1.174416E-05 8.35279E-07
X Variable 3-2.523787E-09 2.656608E-10 Second coefficient

Third coefficient

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A: Calibrating the Wavelength of the HR2000+

7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at
the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.
Keep these values at hand.

Saving the New Calibration Coefficients: USB

Mode
Ocean Optics programs wavelength calibration coefficients unique to each HR2000+ onto an EEPROM
memory chip in the HR2000+.
You can overwrite old calibration coefficients on the EEPROM if you are using the HR2000+ via the
USB port.

► Procedure
To save wavelength calibration coefficients using the USB mode, perform the following steps:
1. Ensure that the HR2000+ is connected to the PC and that you have closed all other applications.
2. Point your browser to http://oceanoptics.com/support/software-downloads/ and scroll down to
Microcode. Select USB EEPROM Programmer.
3. Save the setup file to your computer.
4. Run the Setup.exe file to install the software. The Welcome screen appears.
5. Click the Next button. The Destination Location screen appears.
6. Accept the default installation location, or click the Browse button to specify a directory. Then,
click the Next button. The Program Manager Group screen appears.
7. Click the Next button. The Start Installation screen appears.
8. Click the Next button to begin the installation. Once the installation finishes, the Installation
Complete screen appears.
9. Click the Finish button and reboot the computer when prompted.
10. Navigate to the USB EEPROM Programmer from the Start menu and run the software.
11. Click on the desired HR2000+ device displayed in the left pane of the USB Programmer screen.
12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the Calibrating the
Wavelength of the Spectrometer section in this appendix.
13. Repeat Step 12 for all of the new values.
14. Click on the Save All Values button to save the information, and then Exit the USB Programmer
software.
The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
HR2000+.

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 Appendix B

Specifications
Overview
This appendix contains information on spectrometer operation, specifications, and system compatibility.
It also includes accessory connector pinout diagrams and pin-specific information.

How the HR2000+ Works

Below is a diagram of how light moves through the optical bench of an HR2000+ Spectrometer. The
optical bench has no moving parts that can wear or break; all the components are fixed in place at the time
of manufacture. Items with an asterisk (*) are user-specified.

HR2000+ Spectrometer with Components

See HR2000+ Components Table on the following page for an explanation of the function of each
numbered component in the HR2000+ Spectrometer in this diagram.

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B: HR2000+ Specifications

HR2000+ Components Table

Ocean Optics permanently secures all components in the HR2000+ at the time of manufacture. Only
Ocean Optics technicians can replace interchangeable components, where noted.

Item Name Description

SMA Secures the input fiber to the spectrometer. Light from the input fiber enters the
1
Connector optical bench through this connector.

A dark piece of material containing a rectangular aperture, which is mounted directly

behind the SMA Connector. The size of the aperture regulates the amount of light
that enters the optical bench and controls spectral resolution.
2 Slit
You can also use the HR2000+ without a Slit. In this configuration, the diameter of
the fiber connected to the HR2000+ determines the size of the entrance aperture.
Only Ocean Optics technicians can change the Slit.

Restricts optical radiation to pre-determined wavelength regions. Light passes

through the Filter before entering the optical bench. Both bandpass and longpass
3 Filter filters are available to restrict radiation to certain wavelength regions.
Only Ocean Optics technicians can change the Filter.

Focuses light entering the optical bench towards the Grating of the spectrometer.
Collimating
4 Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter,
Mirror
and then reflects off the Collimating Mirror onto the Grating.

Diffracts light from the Collimating Mirror and directs the diffracted light onto the
Focusing Mirror. Gratings are available in different groove densities, allowing you to
5 Grating specify wavelength coverage and resolution in the spectrometer.
Only Ocean Optics technicians can change the Grating.

Receives light reflected from the Grating and focuses the light onto the CCD
Focusing
6 Detector or L2 Detector Collection Lens (depending on the spectrometer
Mirror
configuration).

An optional component that attaches to the CCD Detector. It focuses light from a tall
slit onto the shorter CCD Detector elements.
L2 Detector
The L2 Detector Collection Lens should be used with large diameter slits or in
7 Collection
applications with low light levels. It also improves efficiency by reducing the effects
Lens
of stray light.
Only Ocean Optics technicians can add or remove the L2 Detection Collection Lens.

Collects the light received from the Focusing Mirror or L2 Detector Collection Lens
CCD and converts the optical signal to a digital signal.
8 Detector Each pixel on the CCD Detector responds to the wavelength of light that strikes it,
(UV or VIS) creating a digital response. The spectrometer then transmits the digital signal to the
spectrometer operating software application.

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 B: HR2000+ Specifications

HR2000+ Specifications
The following sections provide specification information for the CCD detector in the HR2000+, as well
as the HR2000+ Spectrometer itself.

CCD Detector Specifications

Specification Value

Detector Sony ILX-511B linear silicon CCD array

No. of elements 2048 pixels

75 photons per count at 400 nm

Sensitivity
41 photons per count at 600 nm

Pixel size 14 µm x 200 µm

Pixel well depth 65 Ke-

Signal-to-noise ratio 250:1 (at full signal)

A/D resolution 14 bit

Dark noise 3.2 RMS counts

Corrected linearity >99.8%

Maximum pixel rate Rate at which pixels are digitized is 1 MHz

HR2000+ Spectrometer
Specification Value

Dimensions 148.6 mm x 104.8 mm x 45.1 mm

Weight 570 g

Power consumption 220 mA @ 5 VDC

Detector 2048-element linear silicon CCD array

Detector range 200-1100 nm

Gratings 14 gratings available

Entrance aperture 5, 10, 25, 50, 100 or 200 µm wide slits

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B: HR2000+ Specifications

Specification Value

Order-sorting filters Installed longpass and bandpass filters

Focal length f/4, 101 mm

Optical resolution Depends on grating and size of entrance aperture

Stray light <0.05% at 600 nm; <0.10% at 435 nm

8
Dynamic range 2 x 10 (system); 1300:1 for a single acquisition

Fiber optic connector SMA 905 to single-strand optical fiber (0.22 NA)

Data transfer rate:

USB 2.0 Port Full scans into memory every 1 millisecond
Serial Port Full scans into memory every 600 milliseconds

Integration time 1 ms to 65 seconds

USB 2.0, 480 Mbps (USB 1.1 compatible); RS-232 (2-wire); SPI
Interfaces 2
(3-wire); I C Inter-Integrated Circuit 2-wire serial bus

Onboard GPIO 10 user-programmable digital I/Os

Analog channels One 13-bit analog input and one 9-bit analog output

System Compatibility
The following sections provide information on hardware and software requirements for the HR2000+.

Compatibility for Desktop or Notebook PCs

To use the HR2000+, you must have a PC that meets the following minimum requirements:
• Operating systems: Windows 2000/Vista/XP, Mac OS X, or Linux with USB port; any 32-bit or
64-bit Windows OS with serial port
• Computer interfaces: USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud
• Peripheral interfaces: I2C inter-integrated circuit

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 B: HR2000+ Specifications

30-Pin Accessory Connector Pinout

The HR2000+ features a 30-pin Accessory Connector, located on the side of the unit as shown:

Location of HR2000+ 30-Pin Accessory Connector

30-Pin Accessory Connector Pinout Diagram

When facing the 30-pin Accessory Connector on the front of the vertical wall of the HR2000+, pin
numbering is as follows:
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
USB
Port
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29

30-Pin Accessory Connector Pinout Diagram

30-Pin Accessory Connector – Pin Definitions and

Descriptions
The following table contains information regarding the function of each pin in the HR2000+’s 30-Pin
Accessory Connector:

Pin
Function Input/Output Description
#

1 RS232 Rx Input RS232 receive signal – Communicates with a PC over DB9 Pin 3

2 RS232 Tx Output RS232 transmit signal – Communicates with a PC over DB9 Pin 2

General purpose software-programmable, digital input/output

3 GPIO (2) Input/Output
(channel number) Reserved

4 V5_SW Output Regulated 5 Volt power pin – Supplies 50 mA (maximum)

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B: HR2000+ Specifications

Pin
Function Input/Output Description
#

5 Ground Input/Output Ground

2 2 2
6 I C SCL Input/Output I C clock signal for communication to other I C peripherals

General purpose software-programmable, digital input/output

7 GPIO (0) Input/Output
(channel number) Reserved
2 2 2
8 I C SDA Input/Output I C data signal for communication to other I C peripherals

General purpose software-programmable, digital input/output

9 GPIO (1) Input/Output
(channel number) Reserved

Ext. TTL input trigger signal – See External Triggering Options

10 Input
Trigger In document for information.

General purpose software-programmable, digital input/output

11 GPIO (3) Input/Output
(channel number) Reserved

Input power pin for HR2000+ – When operating via USB, this pin
VCC, VUSB,
12 Input or Output can power other peripherals – Ensure that peripherals comply with
or 5VIN
USB specifications

13 Reserved Output Reserved

Input power pin for HR2000+ – When operating via USB, this pin
VCC, VUSB,
14 Input or Output can power other peripherals – Ensure that peripherals comply with
or 5VIN
USB specifications

15 Reserved Input Reserved

General purpose software-programmable, digital input/output

16 GPIO (4) Input /Output
(channel number) Reserved

TTL output pulse used as a strobe signal – Has a programmable

Single
17 Output delay relative to the beginning of the spectrometer integration
Strobe
period

General purpose software-programmable, digital input/output

18 GPIO (5) Input/Output
(channel number) Reserved

19 Reserved Output Reserved

Continuous TTL output signal used to pulse a strobe – Divided down from the
20 Output
Strobe master clock signal

SPI Chip SPI Chip/Device Select signal for communication to other SPI
21 Output
Select peripherals

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 B: HR2000+ Specifications

Pin
Function Input/Output Description
#

General purpose software-programmable, digital input/output

22 GPIO (6) Input/Output
(channel number) Reserved

Analog In
23 Input 13-bit analog-to-digital input with a 0-5V range. For future use.
(0-5V)

Analog Out
24 Output 9-bit programmable output voltage with a 0-5V range
(0-5V)

Lamp TTL signal driven Active HIGH when the Lamp Enable command
25 Output
Enable is sent to the spectrometer

General purpose software-programmable, digital input/output

26 GPIO (7) Input/Output
(channel number) Reserved

27 Ground Input/Output Ground

General purpose software-programmable, digital input/output

28 GPIO (8) Input/Output
(channel number) Reserved

29 Ground Input/Output Ground

General purpose software-programmable, digital input/output

30 GPIO (9) Input/Output
(channel number) Reserved

30-Pin J2 Accessory Connector - Part Numbers

The part numbers for the 30-pin accessory connector on the HR2000+ Spectrometer are as follows:
• The connector is Pak50™ model from 3M Corp. Headed Connector – Part Number
P50–030P1–RR1–TG.
• The mating connector is Part Number P50–030S–TGF.
• Mating the two components requires two 1.27 mm (50 mil) flat ribbon cables (3M 3365 Series is
recommended).
If you are customizing your HR2000+ Spectrometer system or configuring External Triggering, you may
need these part numbers to complete your setup.

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B: HR2000+ Specifications

HR2000+ 15-Pin Accessory Cable Pinout

Pin # Description Pin # Description

1 Single_strobe 9 GPIO-9

2 ContStrobe 10 GND_SIGNAL

3 V5_SW 11 SDA

4 ExtTrigIn 12 SCL

5 ExtTrigIn 13 LampEnable

6 GPIO-8 14 A_IN

7 A_OUT 15 GPIO-7

8 ExtTrigIn

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 Index
Numbers D
15-pin accessory cable detector, 19
pinouts, 24 Detector Collection Lens, 18
30-pin accessory connector document
diagram, 21 audience, iii
part numbers, 23 purpose, iii
pin definitions, 21 summary, iii

A E
accessories, 6 EEPROM, 2
Accessories, 3 External Triggering, 6

F
accessory connector
pinout, 21

B filter, 18
focusing mirror, 18

G
breakout box, 3

C
grating, 18

I
Calibrating, iii, 11
calibration, 11
preparing for, 12
procedure, 12
calibration coefficients Installation, 5
saving in USB mode, 14 Serial Port mode, 6
CCD, 19 USB mode, 5
CCD Detector, 18 installed filter, 18
collimating mirror, 18 Interface, 3

L
compatibility, 20
Desktop or Notebook PCs, 20
Components Table, 18
configuration, 6
L2 Detector Collection Lens, 18
Lens, 18

294-00000-000-02-201502 23
Index

M T
memory chip, 2 Triggering, 6
mirror, 18 troubleshooting

O
Linux systems, 9
Mac systems, 8
Windows systems, 7
Troubleshooting, 7
OceanView, 2
Options
Interface, 3 U
P upgrades, iv
USB mode, 5

packing list, 3
power supply (external), 2 W
product-related documentation, iii

S
warranty, iv
Wavelength Calibration
about, 11
Wavelength Calibration Data File, 3
Sampling Wavelength Calibration Data Sheet, 3
Accessories, 3 what's new, iii
System, 2
Serial Port mode, 6
setup, 5
shipment components, 3
slit, 18
SMA Connector, 18
specifications, 17, 19
detector, 19
spectrometer, 19
spectrometer operation, 17
spectroscopic accessories, 6
System Requirements, 2

24 294-00000-000-02-201502