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BT503 Assignment 2

The document discusses several topics related to genetic engineering and cloning: 1. A cloning vector contains sites for DNA replication, gene selection markers, restriction enzyme sites, ability to be transmitted, and promoters for gene expression. 2. Blue/white screening uses bacterial strains to identify recombinant bacteria, where cells with recombinant DNA appear white and those only with the vector appear blue. 3. Quorum sensing allows microbes to control processes like biofilm formation and virulence based on cell-cell communication and population density. 4. Ballistic bombardment, or biolistics, genetically transforms plants by coating DNA onto metal particles and shooting them into target cells, where the DNA integrates into the host chromosomes.

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63 views2 pages

BT503 Assignment 2

The document discusses several topics related to genetic engineering and cloning: 1. A cloning vector contains sites for DNA replication, gene selection markers, restriction enzyme sites, ability to be transmitted, and promoters for gene expression. 2. Blue/white screening uses bacterial strains to identify recombinant bacteria, where cells with recombinant DNA appear white and those only with the vector appear blue. 3. Quorum sensing allows microbes to control processes like biofilm formation and virulence based on cell-cell communication and population density. 4. Ballistic bombardment, or biolistics, genetically transforms plants by coating DNA onto metal particles and shooting them into target cells, where the DNA integrates into the host chromosomes.

Uploaded by

Umair Afzal
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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MC200404401 NOSHEEN KANWAL

BT503 ASSIGNMENT # 2
Q1: Describe different parts of a cloning vector?
 Site for DNA replication .
 Gene marker for gene selection.
 Unique restriction endonuclease (RE) site.
 Transmissability.
 Promoters for gene expression.
Q2: Describe Blue/White Screening method for recombinants analysis?

Blue–white screen is a screening strategy that takes into consideration the quick and
advantageous recognition of recombinant microscopic organisms in vector-based atomic cloning
tests. This technique for screening is typically performed utilizing a reasonable bacterial strain
yet different organic entities for example yeast may likewise be utilized. DNA of change is
ligated into a vector. The vector is then embedded into a capable host cell suitable for change
which are then filled within the sight of X-lady. Cells changed with vectors containing
recombinant DNA will deliver white states cells changed with non-recombinant plasmids (for
example just the vector) develop into blue provinces.

Q3: Define Quorum sensing?

Quorum sensing (QS) is a correspondence component between microbes that permits


explicit cycles to be controlled for example biofilm arrangement harmfulness factor articulation
creation of optional metabolites and stress transformation instruments for example bacterial
rivalry frameworks including discharge frameworks.

Q4:Explain ballistic bombardment method?

ballistic bombardment or biolistics is an ordinarily involved strategy for hereditary change


of plants and different living beings. A huge number of DNA-covered metal particles are taken
shots at target cells or tissues utilizing a biolistic gadget or quality weapon. The DNA elutes off
the particles that cabin inside the cells and a piece might be steadily joined in the host
chromosomes. A convention for the age of transgenic grapevines by means of biolistic change of
embryogenic cell suspension societies is itemized in this part. In an ordinary test, transient
quality articulation found the middle value of almost 8000 "hits" per barraged plate. Five months
later barrage, there were almost five putative transgenic incipient organisms per assaulted plate.
About portion of the undeveloped organisms were recovered into affirmed transgenic plants. The
fundamental siege strategies depicted are appropriate to a wide scope of plant genotypes,
particularly those for which embryogenic cell societies are accessible. All clients of molecule
assault innovation will track down various valuable tips to augment the accomplishment of
change.
Q5:What is 35S CaMV?

The 35S CaMV promoter is for the most part viewed as a solid constitutive promoter1 and
it works with significant degree of RNA record in a wide assortment of plants, including plants
well external the host scope of the virus2.

CaMV is an aphid-spread plant microorganism that influences plants in the


Brassicaceae family. Brassica, the most financially significant sort inside the Brassicaceae
family, is made out of 37 species, including cabbage, cauliflower, turnip, Brussel sprouts,
arugula, mustard greens, daikon, watercress, and rapeseeds assortments.

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