Kenshin R.

Morales BSBIO 1-2

ANALYTICAL CHEMISTRY
ACTIVITY NO. 01
A B C
2.5 - 6.25 70.10 - 4,914.01 0.792 - 0.6273
3.1 - 9.61 70.22 - 4,930.8484 0.794 - 0.6304
3.1 - 9.61 70.24 - 4,933.6576 0.812 - 0.6593
3.3 - 10.89 ∑𝑋1 = 210.56 2
∑𝑋1 = 0.900 - 0.81
3.5 - 12.25 14,778.516 ∑𝑋1 = 3.298 ∑𝑋1 2 = 2.727
∑𝑋1 = 15.5 ∑𝑋1 2 = 48.61

A.
a. Mean d. Standard Deviation e. Coefficient of Variation
𝑋1 𝑠
x̄ = CV= x 100%
𝑛 2(∑𝑋1 )2 x̄
∑𝑋1 −
15.5 s= √ 𝑛
0.3742
= 𝑛−1
5 = 3.1
x 100%

x̄ = 3.1 (15.5)2 CV= 12.0710 %

48.61−
b. Median= 3.1 =√ 5
5−1

c. Spread
48.61− 48.05
=√
W= HV-LV 4

= 3.5 - 2.5 = √0.14

W= 1 s= 0.3742

e. Coefficient of Variation
𝑠
CV= x 100%
x̄
0.3742
= 3.1
x 100%

CV= 12.0710 %

MARCH 31, 2022

B
a. Mean d. Standard Deviation e. Coefficient of Variation
𝑋1 𝑠
x̄ = (∑𝑋1 )2 CV= x 100%
𝑛 2
∑𝑋1 − x̄
210.56
s= √ 𝑛
0.0758
𝑛−1
= = 70.1867 x 100%
3

x̄ = 70.1867 (210.56)2 CV= 0.1079 %

14,778.516−
=√ 3
b. Median= 70.22 3−1

c. Spread 14,778.516 − 14,778.5045

=√ 2
W= HV-LV
= 70.24 – 70.10 = √0.00575

W= 0.14 s= 0.0758

C.
a. Mean d. Standard Deviation e. Coefficient of Variation
𝑠
𝑋1 CV= x 100%
x̄ = ∑𝑋1 2 −
(∑𝑋1 )2 x̄
𝑛
s= √ 𝑛
0.051
3.298 𝑛−1 = 0.8245 x 100%
= 4

(3.298)2
CV= 6.1856 %
x̄ = 0.8245 2.727−
=√ 4
4−1
b. Median= (0.794 + 0.812)/2

= 0.803 2.727− 2.7192

=√ 3
c. Spread
W= HV-LV = √0.0026

= 0.900 – 0.792 s= 0.051

W= 0.108

MARCH 31, 2022

II.
B=70.05 C=0.830
A=3.0
a. Absolute Error a. Absolute Error
a. Absolute Error
AE= 𝑋𝐸 - 𝑋𝑇 AE= 𝑋𝐸 - 𝑋𝑇
AE= 𝑋𝐸 - 𝑋𝑇
AE= 70.10 – 70.05 = 0.05 AE= 0.792 – 0.830 = 0.038
AE= 2.5 – 3.0 = 0.5
AE= 70.22 – 70.05 = 0.17 AE= 0.794 – 0.830 = 0.036
AE= 3.1 – 3.0 = 0.1
AE= 70.24 – 70.05 = 0.19 AE= 0.812 – 0.830 = 0.018
AE= 3.1 – 3.0 = 0.1
AE= 0.900 – 0.830 = 0.07
AE= 3.3 – 3.0 = 0.3
AE= 3.5 – 3.0 = 0.5
b. Relative Error b. Relative Error b. Relative Error
𝑋𝐸 − 𝑋𝑇 𝑋 −𝑋 𝑋𝐸 − 𝑋𝑇
RE= | |𝑥 100 % RE= | 𝐸𝑋 𝑇 | 𝑥 100 % RE= |
𝑋𝑇
|𝑥 100 %
𝑋𝑇 𝑇
2.5 70.10 0.792
2.5 − 3.0 70.10 − 70.05 0.792 − 0.830
RE= | |𝑥 100 % RE= | 70.05 | 𝑥 100 % RE= |
0.830
|𝑥 100 %
3.0
= 16.6667 % = 0.0714 % = 4.5783 %
3.1 70.22 0.794
3.1 − 3.0 70.22 − 70.05 0.794 − 0.830
RE= | 3.0 | 𝑥 100 % RE= | 70.05 | 𝑥 100 % RE= | 0.830
|𝑥 100 %
= 3.3333 % = 0.2427 % = 4.3373 %
3.1 70.24 0.812
3.1 − 3.0 70.24 − 70.05 0.812 − 0.830
RE= | 3.0 | 𝑥 100 % RE= | 70.05 | 𝑥 100 % RE= |
0.830
|𝑥 100 %
= 3.3333 % = 0.2712 % = 2.1687 %
3.3 0.900
3.3 − 3.0 0.792 − 0.830
RE= | |𝑥 100 % RE= |
0.830
|𝑥 100 %
3.0
= 10 % = 8.4337 %
3.5
3.5 − 3.0
RE= | 3.0
|𝑥 100 %
= 16.6667 %

III.
a. 5.75 (± 0.03) + 0.833 (± 0.001) – 8.021 (± 0.001)

+5.75 (± 0.03) Sy= √𝑆𝑎2 + 𝑆𝑏2 + 𝑆𝑐 2

+0.833 (± 0.001) = √(± 0.03)2 + (± 0.001)2 + (± 0.001)2
-8.021 (± 0.001) = √0.000902
y = -1.438 Sy=? Sy= ± 0.03

-1.438 (± 0.03)

MARCH 31, 2022

b. 66.2 (± 0.3) • 1.13 (± 0.02) x 𝟏𝟎−𝟏𝟕

𝑆𝑦 𝑆𝑎 𝑆𝑏 𝑆𝑐 𝑎∙𝑏
𝑦
= √( 𝑎 )2 + ( 𝑏 )2 + ( 𝑐 )2 y= 𝑐
=a·b
= √(
(± 0.3 2
) + (
± 0.02 x 10−17 2
) = 66.2 (1.13 x 10−17 )
66.2 1.13 x 10−17 y= 7.4806 x 10−16
= √2.0537𝑥10−05 + (3.1326𝑥10−72 )
𝑆𝑦
𝑦
= 4.5318 𝑥10−03

Sy= 4.5318 𝑥10−03 (7.4806 x 10−16)

Sy=± 3.3901𝑥10−18

7.4806 x 10−16 (± 3.3901𝑥10−18 )

c. 251 (± 1) • (𝟖𝟔𝟎 (± 𝟐) )/(𝟏. 𝟔𝟕𝟑 (± 𝟎. 𝟎𝟎𝟔))

𝑆𝑦 𝑆𝑎 𝑆𝑏 𝑆𝑐
𝑦
= √( 𝑎 )2 + ( 𝑏 )2 + ( 𝑐 )2

±1 ±2 ± 0.006 2
= √(251)2 + (860)2 + ( 1.673
)

= √(1.59𝑥10−05 ) + (5.41𝑥10−06 ) + (1.29𝑥10−05 )

= √(3.421𝑥10−5 )
𝑆𝑦
𝑦
= 0.0058 y=
𝑎∙𝑏
𝑐
251 𝑥 860
Sy= (0.0058) (129,025.7023) =
1.673
y= 129,025.7023
Sy= ±748.3491

129,025.7023(±748.3491)

𝑆𝑎
d. log 2.56 (± 𝟎. 𝟓) x 𝟏𝟎−𝟎𝟔 Sy= 0.434 𝑎
y = 𝑙𝑜𝑔10 𝑎 ± 0.5x 10−06
=0.434
2.56 x 10−06
= log (2.56 x 10−06 ) Sy= ±0.0848
y = -5.5918

-5.5918 (±0.0848)

MARCH 31, 2022

 e. antilog [0.1 (± 0.01)]
𝑆𝑦
= 2.303 Sa
𝑦
= 2.303 (± 0.01)
= 0.02303 (1.2589)
Sy= ±0.029

y= 𝑎𝑛𝑡𝑖𝑙𝑜𝑔10𝑎
= antilog10(0.1)
y= 1.2589

1.2589 (±0.029)

IV.
Sample Ca λ(nm) 𝑋1 2
1 336.19 113,023.7161

2 443.49 196,683.3801
3 445.47 198,443.5209
4 452.69 204,928.2361

5 643.91 414,620.0881
∑𝑋1 =2,321.75 ∑𝑋1 2 = 1,127,698.941

a. Mean
``````````````````````````````````````
𝑋
x̄ = 𝑛1
2,321.75
= d. Variance = 𝑠 2 f. Coefficient of Variation
5 𝑠
x̄ = 464.35 V= 111.34892 CV= x 100%
x̄
V= 12,398.5775 111.3489
b. Median= 445.47 = 464.35 x 100%
e. RSD @ppt CV= 23.9795%
c. Standard Deviation 𝑠 g. Range
RSD= x̄ x 1000 ppt
(∑𝑋1 )2
∑𝑋1 2 − W= HV-LV
s= √ 𝑛
=
111.3489
x 1000 ppt
𝑛−1 464.35 = 643.91 – 336.19
W= 307.72
1,127,698.941−
(2,321.75)2 RSD = 239.7952 ppt
=√ 5
5−1

1,127,698.941− 1,078,104.613
=√ 4
= √12,398.582
s = 111.3489 nmCaλ

MARCH 31, 2022

 V.

Metabolite VIP
a. Mean c. Glycine
Ethanolamine 1.82 – 3.3124 𝑋1
x̄ = d. Galacturonate
Phenylethanolamine 1.67 – 2.7889 𝑛
19.28
Glycolate 2.3 - 5.29 = e. Galacturonate
11
Glycerol 1.74 – 3.0276 x̄ = 1.7527
Galacturonate 1.32 – 1.7424 b. Standard Deviation
Stearate 1.34 – 1.7956
2 (∑𝑋1 )2
∑𝑋1 −
Succinate 1.6 - 2.56 s= √ 𝑛
𝑛−1
Fumarate 1.47 – 2.1609
Isoleucine 1.75 – 3.0625 35.1088−
(19.28)2

Serine 1.78 – 3.1684 =√ 11

11−1
Glycine 2.49 – 6.2001
35.1088− 33.7926
∑𝑋1 =19.28 =√ 10
∑𝑋1 2 = 35.1088 = √0.13162
s= 0.3628

MARCH 31, 2022