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07 Calibration or Response Factor Calib

The document defines calibration factor and response factor as measures of the chromatographic or mass spectral response of an analyte relative to its concentration or internal standard. It provides equations to calculate the average response factor or calibration factor from multiple standards, and equations to determine concentration from these factors using external or internal standard methods. For a calibration to be considered linear, the relative standard deviation of the factors must be less than or equal to 20% or 25%.

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287 views13 pages

07 Calibration or Response Factor Calib

The document defines calibration factor and response factor as measures of the chromatographic or mass spectral response of an analyte relative to its concentration or internal standard. It provides equations to calculate the average response factor or calibration factor from multiple standards, and equations to determine concentration from these factors using external or internal standard methods. For a calibration to be considered linear, the relative standard deviation of the factors must be less than or equal to 20% or 25%.

Uploaded by

Rui Sheena
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Response/Calibration Factor

Definitions
Calibration Factor: A measure of the chromatographic response of a target analyte relative to the mass injected. A measure of the relative mass spectral response of an analyte compared to its internal standard.

Response Factor:

Ref: EPA R3 Quality Manual Rev 3 (1/12/04)

Definitions
Calibration Factor: A measure of the chromatographic response of a target analyte relative to the mass injected. A measure of the relative mass spectral response of an analyte compared to its internal standard.

Response Factor:

Ref: EPA R3 Quality Manual Rev 3 (1/12/04)

Definitions-Cont.
Each calibration or response factor represents the slope of the line between the response for a given standard and the origin. The average calibration factor or response factor of the standards for each analyte is then used to calculate the concentration of the sample.
Ref: EPA R3 Quality Manual Rev 3 (1/12/04)

Criteria
When the variation, measured as the relative standard deviation (RSD) of the factors, is less than or equal to 20%, then the slopes of the lines for each standard are sufficiently close to one another that the use of the linear model is generally appropriate over the range of standards that are analyzed.
Ref: SW-846, 8000C, Section 11.5.1

Criteria-Cont.
A relative standard deviation (RSD) of 25% or less is considered linear.
Ref: Protocol for EPA Approval of Alternate Test Procedures for Organic and Inorganic Analytes in Wastewater and Drinking Water, EPA-821B-98-002, March 1999

Response/Calibration Factor Equations


External Standard Equation CF = (Ax)/(Cx) or Internal Standard Equation RF = ((Ax)(Cis))/((Ais)(Cx))
Where: Ax = Area of the compound Cx = Concentration of the compound Ais = Area of the internal standard Cis = Concentration of the internal
standard

Response/Calibration Factor Statistical Equations


Average RF or CF: RFAVE = (S RFi / n )

Standard Deviation (s): s = { [ S (RFi - RFAVE )2 ] / (n-1) } Relative Standard Deviation (RSD): Where: RSD = s / RFAVE *100

n = number of pairs of data RFi = Response Factor for each level RFAVE = Average of all the response factors S = the sum of all the individual values

In the equations above RF can be replaced with CF

Response/Calibration Factor Equations for Concentration


External Standard Equation
Cx = Ax/CFAVE or

Internal Standard Equation


Cx = ((Ax)*(Cis)) / ((Ais)*(RFAVE))

Benefits
1. Assumes linearity through the origin, no negative calculated concentrations. 2. Simple calculation.

Benefits
1. Assumes linearity through the origin, no negative calculated concentrations. 2. Simple calculation.

Disadvantages
1. Linearity of the curve is required.
The main benefit is that the calculation is very simple.

2. May not reflect actual detector response curve.

Disadvantages
1. Linearity of the curve is required.
The main benefit is that the calculation is very simple.

2. May not reflect actual detector response curve.

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