HumaClot Pro

| Service Manual

Cat No. 15800/2

REVISION LIST OF THE MANUAL
Rev. /DATE. REVISION DESCRIPTION
01/2016-09 First Edition
02/2019-03 Update Service Software
03/2019-10 Closing Software, diverse changes

SYSTEM VERSION

1.1 Software

COPYRIGHT

Copyright 2019, Human Gesellschaft für Biochemica und Diagnostica mbH, Wiesbaden,
Germany. All rights reserved.

No part of this documentation may be reproduced in any form, nor processed, copied or distrib-
uted by means of electronic systems, without prior permission of HUMAN in writing. Since all
precautionary measures were taken into account in producing these operating instructions, the
manufacturer accepts no responsibility for any errors or omissions. This includes any liability for
damage that could arise from possible incorrect operation based on this information. Subject to
changes without notice as result of technical development.

SERVICE UND SUPPORT

CONTENTS

TABLE OF CONTENTS

1  SAFETY INSTRUCTIONS 9
1.1 INTRODUCTION 9
1.2  USER WARRANTY 9
1.3  INTENDED USE OF THE INSTRUMENT 9
1.4  GENERAL SAFETY WARNINGS 10
1.5  DISPOSAL MANAGEMENT CONCEPT 10
1.6  BIOHAZARD WARNING 11
1.7  INSTRUMENT DISINFECTION 11
1.8 REPAIRS 11

2  SYSTEM DESCRIPTION 13
2.1  ANALYZER DESCRIPTION 13
2.1.1  Touch Screen 14
2.1.2  Working Plate 16
2.1.3  Cuvette Ring Position 18
2.1.4  Sample Area 19
2.1.5 Dilutor 21
2.1.6 Pipettor 21
2.1.7  Rinsing System 22
2.1.8  Peripheral equipment and mains connection 24
2.2 SOFTWARE 26
2.2.1  Software control via touch screen 27
2.2.2  Software Overview 31
2.2.3  Program Menus 32
2.3  MEASURING PRINCIPLE 37

3 INSTALLATION 39
3.1  GENERAL SETUP 39
3.2  REMOVING THE TRANSPORT PROTECTIONS 40
3.3  ASSEMBLY OF THE ANALYZER 42
3.3.1  Connect the container sensors 42
3.3.2  Connect an USB Memory Device 44
3.3.3  Connect an external printer 44
3.3.4  Connect an external Barcode Scanner (option) 45
3.3.5  Protection with power safety socket (surge protector) and
online UPS 46

4  REPACKING AND DECONTAMINATION 55

 4.1 REPACKING 55
4.1.1  Transport Safety 55
4.1.2 Packaging 57
4.2  DECONTAMINATION AND PREPARE FOR SHIPMENT 60

5  SERVICE MENU 61
5.1  ACCESSING THE SERVICE SOFTWARE 61
5.2  REPORT SECTION 62
5.2.1  Button SHOW 62
5.3  DATA SECTION 62
5.3.1  Button USB-STICK 62
5.3.2  Button PARAMETERS 63
5.3.3  Button SOFTWARE 64
5.3.4  Button SERIAL NUMBERS 64
5.3.5  Date and Time 65
5.3.6  Access Mode 66
5.4  ELECTRONIC SECTION 67
5.4.1  Button PWR. SUPPLY 67
5.4.2  Button PHOTOMETER 68
5.4.3  Button CHIPCARD 72
5.4.4  Button BARCODE SCANNER 72
5.4.5  Button CONTAINER SENSORS 74
5.4.6  Button DISPLAY 75
5.4.7  Button CALIBRATE TOUCH 75
5.5  MECHANICS SECTION 77
5.5.1  Button TEACH-IN 77
5.5.2  Button STIRRER  80
5.5.3  Button ROTOR 81
5.5.4  Button CALIBRATE 82
5.5.5  Button CHECK Ring 82
5.5.6  Button TEACH-IN 83
5.5.7  Button PIPETTOR 84
5.5.8 Dilutor 90
5.5.9 Shield 92
5.5.10 Selftest 93
5.5.11 Webserver 101

6 MAINTENANCE 107
6.1  MAINTENANCE FREQUENCY 107
6.2 EQUIPMENT 110
CONTENTS

6.3 MAINTENANCE 110

7 TROUBLESHOOTING 113
7.1  ERROR MESSAGES SYSTEM 113
7.1.1  Movement YXZ 113
7.1.2  Measuring rotor 115
7.1.3 Photometer 116
7.1.4 Dilutor 116
7.1.5  Wash station / Tubing system 117
7.1.6 Temperature 118
7.1.7 Miscellaneous 119
7.2 GENERAL 119
7.3  LIQUID SYSTEM 120
7.3.1  Pipettor water jet 120
7.3.2  Pipettor rinse (In case of stocked Pipettor tip) 120
7.3.3  DI Water System rinse (in case no DI Water flow) 122

8 DIAGRAMS 125
8.1  ANALYZER COMPONENTS 125
8.2  WIRING DIAGRAMS 126
8.2.1  Power Supply Unit 126
8.2.2 Cardreader 127
8.2.3  Dilutor / Pump Unit 128
8.2.4  Measrotor and Photometer 129
8.2.5  Reagent Stirrer, Cover Sensor and Barcode reader 130
8.2.6  XYZ Unit 131
8.2.7 PE 132
8.2.8  Liquid System 133

9  HARDWARE AND ADJUSTMENTS 135

9.1  POWER FILTER SUPPRESSES 135
9.2  PCB BOARDS 135
9.2.1  PCB Main board 135
9.2.2  PCB XYZ Servo 140
9.2.3  PCB Photometer 140
9.3  PCB HOUSING LOCK 141
9.3.1  PCB Pumps 141
9.3.2  PCB Valve 142
9.4 MECHANIC 143
 9.4.1  Measuring rotor 143
9.4.2 Dilutor 145
9.5  INTERFACE EXTERN 146
9.5.1  LAN extern 146
9.5.2 RS-232 146
9.5.3 USB 147
9.5.4  Barcode scanner extern (Data) 148
9.5.5  Interface Sensor DI Water extern 149
9.5.6  Interface Sensor DI WaSTE extern 150

10  REPLACEMENT OF PARTS 151

10.1  DISMANTLE HOUSING 151
10.1.1  Required tools 151
10.1.2  Switch off analyzer 151
10.1.3  Secure Pipettor 152
10.1.4  Remove all loose parts 152
10.1.5  Remove reagent area 153
10.1.6  Unfasten reagent stirrer plugs 153
10.1.7  Remove cover measuring rotor 154
10.1.8  Remove front cover 154
10.1.9  Remove top cover part 1 155
10.1.10  Remove pipettor tubing from top cover 155
10.1.11  Remove top cover part 2 156
10.1.12  Completely opened instrument 156
10.2 PIPETTOR 157
10.2.1  Required tools 157
10.2.2  Unfasten tubing 158
10.2.3  Unfasten cover 158
10.2.4  Unfasten cable Z-motor 159
10.2.5  Unfasten flex cable 159
10.2.6  Unfasten Pipettor 160
10.2.7  Unfasten guide rail 1 160
10.2.8  Unfasten guide rail 2 161
10.2.9  Turn pipettor 161
10.2.10  Remove the pipettor 162
10.2.11  Example: Secure guide rail 162
10.3  DILUTOR AND PUMP ANGLE 163
10.3.1  Required told 163
10.3.2  Unfasten mounting screws) 163
10.3.3  Unplugging cable 164
CONTENTS

10.3.4  Remove cable holder and grounding cable 165

10.3.5  Disconnect waste tubing from waste pump 165
10.3.6  Remove pipettor tubing 166
10.3.7  Unplug DI and Waste water sensor cables 166
10.4 DILUTOR 167
10.4.1  Required tools 167
10.4.2  Removing mounting screws of Dilutor 167
10.4.3  Removing fixing screw of Syringe holder 168
10.4.4  Remove Tubing DI water pump and Syringe holder 168
10.4.5  Unplug valve cable from Dilutor PCB 169
10.4.6  cable from Dilutor PCB 169
10.4.7  Removed Dilutor module 169
10.5  DI AND WASTE WATER PUMP 170
10.5.1  Required tools 170
10.5.2  Unplug DI water pump from pump PCB 170
10.5.3  Disconnect Di water outlet tubing 171
10.5.4  Disconnect the tubing from the waste water pump 171
10.5.5  Removing screws of waste water pump 172
10.6  DISPLAY AND TOUCH SCREEN 172
10.6.1  Required tools 172
10.6.2  Removing cover 173
10.6.3  Removing display 173
10.6.4  Disconnect cables from Display 174
10.6.5  Already removed Display 174
10.7  CARD READER 175
10.7.1  Required tools 175
10.7.2  Unfasten screws 175
10.8  POWER SUPPLY 176
10.8.1  Required tools 176
10.8.2  Unfasten cables 176
10.8.3  Open power supply cover 177
10.8.4  Remove power supply and dejam coil 177
10.8.5  Already removed power supply and dejam coil 178
10.9  CONNECTION PANEL SENSORS 178
10.9.1  Required tools 178
10.9.2  Disconnect Sensors Di and Waste 179
10.9.3  Unfasten tubing’s 179
10.10 VALVE 180
10.10.1  Required Tools 180
10.10.2  Remove mechanical valve 180
 10.10.3  Remove electronic valve 181
10.10.4  Already removed valve 181
10.11  DILUTOR MOTOR 182
10.11.1  Required tools 182
10.11.2  unfasten Cable 182
10.11.3  Remove screws 183
10.11.4  Already removed Dilutor Motor 183
10.12  PCB XYZ SERVO 184
10.12.1  Required tools 184
10.12.2  Unplug cables 184
10.12.3  Removing screws 185
10.13  RINSING STATION REMOVING SCREWS 185
10.13.1  Required tools 185
10.13.2  Remove screws 186
10.13.3  Remove waste tubing 186
10.14  MEASURING ROTOR 187
10.14.1  Required tools 187
10.14.2  Remove screws 187
10.14.3  Unplug marked cables 188
10.14.4  Already removed measuring rotor 188
10.15 PHOTOMETER 189
10.15.1  Required tools 189
10.15.2  Remove screws 189
10.15.3  Unplug marked cables 190
10.15.4  Already removed Photometer 190
10.16  MIXER BOARD 191
10.16.1  Required tools 191
10.16.2  Remove Screws 191
10.16.3  Already removed mixer board 192
10.17  MOTOR X 192
10.17.1  Required tools 192
10.17.2  Remove cables 193
10.17.3  Remove screws at PCB 193
10.17.4  Remove YZ-Traverse 194
10.17.5  Unfasten X-Holder 194
10.17.6  Unfasten X-Motor alignment 195
10.17.7  Set free pin 195
10.17.8  Alignment at assembly 196
10.18  MOTOR Y 196
10.18.1  Required tools 196
10.18.2  Unfasten cables 197
CONTENTS

10.18.3  Unfasten YZ motor holder 197

10.18.4  Set free pin and remove 198
10.19  MOTOR Z 198
10.19.1  Required tools 198
10.19.2  Remove cables 199
10.19.3  Remove YZ motor holder 199
10.19.4  Set free pin and remove

11  LIS INTERFACE

Safety Instructions 9

1  SAFETY INSTRUCTIONS

1.1  Introduction
This manual is considered part of the instrument and must be available to the
operator and the maintenance personnel. For accurate installation, use and
maintenance, please read the following instructions carefully.
In order to avoid damage to the instrument or personal injury, carefully read
the ”GENERAL SAFETY WARNINGS”, describing the appropriate operating pro-
cedures. Please contact your HUMAN authorised local Technical Service in the
event of instrument failure or other difficulties with the instrument.

1.2  User Warranty

HUMAN warrants that instruments sold by one of its authorised representa-
tives shall be free of any defect in material or workmanship, provided that this
warranty shall apply only to defects which become apparent within one year
from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item within this
warranty period at no charge, except for transportation expenses to the point
of repair.
This warranty excludes the HUMAN representative from liability to replace any
item considered as expendable in the course of normal usage, e.g.: lamps, valves,
syringes, glassware, fuses, tubing etc.
The HUMAN representative shall be relieved of any liability under this warranty
if the product is not used in accordance with the manufacturer‘s instructions,
altered in any way not specified by HUMAN, not regularly maintained, used with
equipment not approved by HUMAN or used for purposes for which it was not
designed.

1.3  Intended Use of the Instrument

The instrument must be used for its intended purpose (see paragraph 2). It must
be operated in perfect technical conditions, by qualified personnel, in such [IVD]
working conditions and maintained as described in this manual, in the GENERAL
SAFETY WARNINGS. This manual contains instructions for qualified professional
operators.
 10

1.4  General Safety Warnings

Use only chemical reagents and accessories specified and supplied by HUMAN
and/or mentioned in this manual. Place the product so that it has proper ven-
tilation.
The instrument should be installed on a flat, stationary working surface, that is
free of vibrations.
Do not operate in area with excessive dust.
Operate at temperature and at a humidity level in accordance with the specifi-
cations (see specifaction flyer).
Do not operate this instrument with covers and panels removed.
Use only the power cord specified for this product, with the grounding conduc-
tor of the power cord connected to earth ground.
Use only the fuse type and rating specified by the manufacturer for this instru-
ment.
The use of fuses with improper ratings may pose electrical and fire hazards.
To avoid fire or shock hazard, observe all ratings and markings on the instru-
ment.
Do not power the instrument in environments that are potentially explosive or
at risk of fire.
Prior to cleaning and/or performing maintenance on the instrument, switch off
the instrument and remove the power cord.
Only cleaning materials described in this manual may be used, as other mate-
rials may damage parts. It is recommended to always wear protective clothing
and eye protection while using this instrument.
All warning symbols that appear in this manual must be carefully observed.

1.5  Disposal Management Concept

The applicable local regulations governing disposal must be observed. It is the
user‘s responsibility to arrange for proper disposal of the individual components.
All parts which may contain potentially infectious materials must be disinfect-
ed by suitable, validated procedures (autoclaving, chemical treatment) prior to
disposal. Applicable local regulations for disposal must be carefully observed.
The instruments and electronic accessories (without batteries, power packs etc.)
must be disposed of according to the applicable local regulations for the dispos-
al of electronic components.
Batteries, power packs and similar power sources must be removed from elec-
tric/electronic parts and disposed of in accordance with applicable local regula-
tions.

HumaClot Pro | Service Manual

Safety Instructions 11

1.6  Biohazard Warning

Analytical instruments for in vitro diagnostic application involve the handling of
human samples and controls which should be considered at least potentially in-
fectious. Therefore every part and accessory of the respective instrument which
may have come into contact with such samples must equally be considered as
potentially infectious.
The „BIOHAZARD“ warning label must be affixed to the instrument prior to first
use with biological material!

Figure 1
Biological Hazard Symbol

1.7  Instrument Disinfection

Before performing any servicing on the instrument it is very important to thor-
oughly disinfect all possibly contaminated parts. Before the instrument is re-
moved from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination must be performed by authorised well-trained personnel, and
in observance of all necessary safety precautions.

1.8  Repairs

-- For all the operations described in this manual, wear latex or

nitrile gloves and wash hands thoroughly after completion
of work.
-- Be careful with the sharp edges of the metal sheets after re-
moval of the covers.
 12

HumaClot Pro | Service Manual

SYSTEM DESCRIPTION 13

2  SYSTEM DESCRIPTION
The HumaClot Pro named hereafter as analyzer, is a fully automated photo-op-
tical blood plasma hemostasis instrument capable of performing clotting, chro-
mogenic and immunoassays simultaneously in random access or batch mode
from primary and secondary tubes.

For in-vitro diagnostic use only!

-- Depending on the analyzer model the system is capable to perform a wide

range of coagulometric, chromogenic and immunologic coagulation tests
such as Prothrombin time, activated and partial Thromboplastin time, Fi-
brinogen, derived Fibrinogen and special tests such as single factor assays,
Anti-Thrombin III, Protein C, Protein S, C-Reactive Protein, D-Dimer and oth-
ers based on the wavelength available.

The analyzer is intended for use by trained operators in coagulation labs for
in-vitro diagnostics.

This chapter contains detailed analyzer description, its components and pro-
vides detailed information on how to unpack and install the analyzer the first
time.

2.1  Analyzer description

The analyzer represents a new state of the art development and is a micropro-
cessor-controlled, two-channel photo-optical fully automated blood plasma he-
mostasis analyzer. The analyzer is a modular construction.
 14

Figure 2
Touchscreen
1 Pipettor
2 Lid
3 Cup Array for Sample Cups
(Controls/Calibrators)
4 Reagent Block (removable)
5 Washer / Cleaner Position
6 Rinsing Station
7 Measuring / Cuvette Ring
Rotor
8 Internal Barcode Scanner
(depending on analyzer
type)
9 STAT Positions
10 Sample Positions
11 Smart Card Reader
12 Touch sensitive colour
display
USB Stick Interface (Back)
! Caution: Handling of safety shield: It is recommended to always close the safety shield
during analyzer operation to avoid injuries and damage of moving parts. Open the safety
shield only when the analyzer is not in operation. The safety shield must be opened all the
way to the top.

2.1.1  Touch Screen

The touch screen control panel offers several functions. On one hand, it is de-
signed for the visual display of the entire instrument control software. On the
other hand, it is used for inputting information, acknowledgment of software
messages, display of application and test ranges and for communication with
the user.

The LCD colour touch screen offers a visible area of 17.0x12.5cm and provides a
display resolution of 800x600 pixels.

Its touch sensitivity makes it possible to navigate your way round the software
simply by pressing the surface of the user control panel. Use either your finger
tip or a blunt object, e.g. a touch screen pen designed for this purpose.

HumaClot Pro | Service Manual

SYSTEM DESCRIPTION 15

Figure 3
Touchscreen

Function and Navigation Keys

The touch screen display of the analyzer offer certain

Scroll-up by one window/page
! Note: Never use a sharp object
(finger nail, knife, letter open-
er) to press on the control panel
as it might cause irreparable dam-
Scroll-up by one line
age to the control panel surface.

Scroll-down by one window/page

Scroll down by one line

ESC key to cancel and leave a display/action

OK key to select an action

ESC& Exit key to leave a display without confirming and

saving input
Save&Exit to leave a display and save input

Tools key to enter into a selection menu offering further

actions
 16

Keyboard (alphanumeric / numeric)

Alphanumeric keys, some with special symbols, for text and

numerical information input

Activates upper or lower case

Activates numeric keys and special symbols

Space bar, or overwriting input

Numeric keys, for numerical information input

Exponent key

Short dash key, e.g. to separate two numbers

Decimal or comma key

Arrow keys to move the cursor around the input field

For deleting the input to the left of the cursor

Confirm and save input

Leave input field (keyboard window) without confirming

and saving input

2.1.2  Working Plate

The analyzer is equipped with a working plate which offers all positions to load
routine & STAT samples, reagents, controls & calibrator, washer, cleaner and
cuvette rings. This Working Plate is actually divided into three single areas:

1. Reagent Area, consists of:

Washer & Cleaner Positions,
Reagent Block (removable),
Control & Calibrator Positions
2. Cuvette Ring Position, consists of:
Cuvette Ring / Measuring Rotor,
Wash / Rinsing Position

HumaClot Pro | Service Manual

SYSTEM DESCRIPTION 17

3. Sample Area, consists of:

Routine Sample Cup Positions,
STAT Positions

Reagent Area
The Reagent Area is a combination of different liquid positions at room tem-
perature. The Reagent Area offers positions for Washer and Cleaner, reagent
positions, diluents as well as positions for controls & calibrators.

Figure 4
Reagent Area

W1-W2 2x Washer & Cleaner Positions.

Reagent Block with 15 positions.
(R1-R3) for 15ml reagent vials or 5ml reagent vials with reducing
R1-R15 ring (all position stirred)
(R4-R15) for 5ml reagent vials. The reagent block can be removed
from the working plate for external cooling.
C1-C8 8x Control & Calibrator Positions.

To place control and calibrator vessels, the analyzer uses the controls/calibrator
positions (C1-C8).
The control and calibrator positions accommodate the following tubes:

Type Volume Size Tube

HUMAN-Cup 4ml 61mm x 12.1mm diameter Secondary
Hitachi® Cup 2ml 38mm x 17mm diameter Secondary
 18

2.1.3  Cuvette Ring Position

The measuring module consists of:

-- The Measuring rotor which has 32 cuvette positions. The temperature of the
measuring rotor is set to 38,5°C +/-0.4°C.
-- The measuring rotor contains a triangular slot on the rotor surface for proper
ring placement (triangular arrow).
-- The measuring rotor is equipped with 2 measuring channels, each carrying a
high-resolution LED photometer and reference channels, the photometers
are able to measure at a wavelength of 405nm, 570mn and 740nm.

Figure 5
Measuring Rotor

Figure 6
Cuvette Ring

! Note: When placing the cu-

vette ring into the measuring
rotor make sure the triangular
markings of the cuvette ring
match the same direction as the
triangular slot on the measur-
ing rotor and the cuvette ring is
pushed all the way down.

! Note: Only use the original

cuvette ring and stir bars pro-
vided by the manufacturer to op-
Each well of the cuvette ring contains a 1x4mm stir bar. These stir bars are nec-
essary to obtain accurate measurements due to generating a homogeneous dis-
erate the system, otherwise you tribution of the plasma and the reagent. These stir bars are driven magnetically
may risk wrong measuring results by motors underneath the measuring rotor.
and the warranty will to be lost.

HumaClot Pro | Service Manual

SYSTEM DESCRIPTION 19

2.1.4  Sample Area

For placing samples into the analyzer use the sample positions on the working
plate. Primary tubes and sample cups can be placed into the STAT and sample
positions.

Figure 7
Sample Area

1-3 3x STAT Positions

S1 - S11 1x Sample Rack contains sample positions 1-11
S12 - S22 1x Sample Rack contains sample positions 12-22
The STAT and Sample Positions accommodate the following sample tubes:
Type Volume Size Tube
HUMAN Cup
Hitachi® Cup
4ml
2ml
61mm x 12.1mm diameter
38mm x 17mm diameter
Secondary
Secondary ! Note: Only capless tubes are
allowed. The use of closed
tubes will cause damage to the
Tube-Long:
HumaTube - No Additive 5 ml 75 x 13 mm Primary instrument.
HumaTube - No Additive 7 ml 100 x 13 mm Primary
HumaTube - Na-Citrate 2,7 ml 75 x 13 mm Primary
Sarstedt® Monovette® 92mm x 11.5mm diameter
Beckton Dickinson Vacutainer 5ml 98mm x 12.3mm diameter +Primary
Terumo® Venoject® 99mm x 12.4mm diameter
Tube-Short:
Terumo® Venoject® 2.7ml 65mm x 10.3mm ø
Primary
Sarstedt® Monovette® 3ml 66mm x 11.5mm ø
Beckton Dickinson Vacutainer 4.5ml 75mm x 12.3mm ø
 20

! Note: Sample IDs can be introduced to the analyzer either by using an ex-
ternal barcode scanner (optional available) or by internal barcode scanning.
To read how to connect and operate a barcode scanner, see user manual an
external Barcode Scanner (option).

Figure 8
Internal Barcode Scanner

The hight of the tubes can be adjusted by changing the inside deck of the
Sample Rack.

Figure 9
Sample Rack –
Deck moved in and – Moved out

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SYSTEM DESCRIPTION 21

2.1.5  Dilutor
The analyzer is equipped with a dilutor module fitted with a 250µl syringe and a
selection valve, which switches between dispensing and rinsing mode.

Figure 10
Dilutor Module

2.1.6  Pipettor
The Pipettor automatically executes all test preparations. It aspirates sample
plasma (as well as standards and controls) from the sample positions and pi-
pettes it into a measuring cuvette. It then aspirates one or more reagents from
reagent vials, placed in the reagent block or controls/calibrator area and dis-
penses these into the measuring cuvette.

To prevent contamination, the pipettor is rinsed in the rinsing station after each
pipetting step.

The pipettor is a capacitive pipetting device heated at 37.4°C which is capable to

move into three directions:

-- X-Direction: left and right

-- Y-Direction: backward and forward
-- Z-direction: up and down
 22

Figure 11
Pipettor

2.1.7  Rinsing System

! Note: The distilled water need

to be placed on the same table
hight as the instrument to work
The rinsing system is used to clean the pipettor and the tube system. It contains
of a rinsing station, two pumps (distilled and waste pump), a dilutor and two
containers (distilled water, waste water). The containers can be placed behind
proper. or beside the analyzer.

The distilled water container is marked with a green label, whereas the waste
container is marked with a red label.

Figure 12
Container and level sensors
for distilled water (green)
and waste water (red)

Both containers are fitted with level sensors. When distilled water is empty or
when the waste water container is full, an appropriate message displays on the
screen.

The levels of the liquids will be checked:

-- Upon leaving the run preparation menu

! Note: Prevent DI water filters

from drying out (bottle need
to be filled with DI water). Dried
-- Prior to the start of a run
-- During the run
-- Prior to the start of a calibration
filters can cause reduced water
supply what can cause wrong re- The distilled water tube is internally connected via the distilled water pump to
sults. Dried filters need to be ex- the dilutor valve. Distilled water is used as the system liquid and for washing/
changed by new one. rinsing of the pipettor. The rinsing solution is supplied to the rinsing station and,

HumaClot Pro | Service Manual

SYSTEM DESCRIPTION 23

after several rinsing cycles, pumped into the waste water container by means of
the waste water pump.

Figure 13
Rinsing System

! Note: The distilled water container has to be filled with distilled water only.
Never use tap water or water with a disinfectant! This avoids the correct
functionality of the liquid level detection at the pipettor. The distilled water
used has to fulfil the following specification: Electric conductivity: 4 – 6 µS/cm
pH value: 6.5 – 8.0.

Besides the rinsing station the analyzer offers two positions within the reagent
area, see section Reagent Area, used to place vials with Cleaner and or Washing
Solution.

The Washing Solution is used during intensive rinsing to clean the pipettor from
thrombin containing reagents (thrombin in activator).

The Cleaner on the other hand is used to clean the system to prevent the growth
of algae and bacteria and to remove possible contamination (mainly in the
waste water system and pipettor).
There are different types of rinsing programs available:

!
Type Cycle description Note: Only use the original
Flush 10s System water, 10 sec. rinsing Washing solution and Clean-
System water, 1 sec. rinsing er provided by the manufacturer
Normal
(only available in Reagent Setup) - See chapter 6.2. User Manual
Washer 1 (Pos. W1)
Otherwise you may risk the clog-
-- 3 sec. rinsing with system water ging of the pipettor, liquid detec-
Intensive 1 -- 150µl Washer 1 tion problems or wrong measur-
-- 3 strokes up to 250µl ing results and the warranty will
-- 3 sec. rinsing with system water to be lost.
 24

Washer 2 (Pos. W2)

-- 5 sec. rinsing with system water
Intensive 2 -- 150µl Washer 2
-- 3 strokes up to 250µl
-- 5 sec. rinsing with system water
Washer 1 (Pos. W1)

-- 1.5 sec. rinsing with system water

Intensive 3 -- 100µl Washer 1
-- 5 strokes up to 250µl
-- 1.5 sec. rinsing with system water

Intensive 4 4 sec. rinsing with system water

Cleaner ( Pos. W2)
Cleaning Cycle -- 1 sec. rinsing with system water
(replace Washer 2 by -- 200µl Cleaner
cleaning solution) -- 3 strokes up to 100µl
-- 20 sec. rinsing with system water

2.1.8  Peripheral equipment and mains connection

In general no additional equipment is necessary to operate the analyzer.

The integrated module and system software controls the system and manages
all data handling. However, for external data transfer and storage of parameters
and results the analyzer is equipped with several interfaces.

Using these interfaces the analyzer can be connected to an external computer

using either the RS232C interfaces or the integrated LAN interface. Measured
results, reagent and test-specific data, software screens can even be printed by
means of an external USB printer device connectable to the analyzer or stored
by on an USB memory device.

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SYSTEM DESCRIPTION 25

The back and side plane of the analyzer carries the mains supply connection. The
instrument is designed for mains supply voltages between 100V and 240V, at
50/60Hz.

The analyzer main switch is above the power supply connection. This is accom-
modated in the main input filter that also carries the fuse carrier and the power
plug.

! Note: The analyzer is delivered with an EU Power supply cable. If a dif-

ferent cable has to be used due to different power supply system, the
cable must meet the local regulations (e.g. IEC 320, VDE, CSA-C22.2, No. 21
and No. 49). The max. length of the cable has to be 2.5 m, and the mini-
mum cross section 3 x 1 mm2. A cold device plug is used at the system’s end.
Use only fuses recommended by the manufacturer. Observe the instructions
on the plate located on the back side of the analyzer (see picture below). The
analyzer is set to 230V by the manufacturer, change fuses (supplied in accesso-
ries) in the main input filter, if analyzer will be operated in 115V.

Main supply filter, contains:

-- Fuse carrier
1- -- Power Switch I = ON, O = OFF
-- Main plug
2- 1x USB Device (for factory use only)
3- 2x USB (External printer (top position) and USB-Stick)
4- LAN Interface for HOST connection
5- RS 232C for external barcode scanner
6- RS232C Mini DIN for Service
7- Waste Water (Connection for Level Sensor red)
8- Dist. Water (Connection for Level Sensor green)
 26

2.2  Software
The operating system software interface allows the user to operate the analyzer
as an independent measuring instrument without any peripheral equipment.
The analyzer software is stored in a ROM and is started after the instrument is
switched on. It controls the analyzer through the start-up initialization and con-
trols all functions for analyzer setup and the analysis programme.

Some analyzer software application areas are protected with an additional PIN
code (password). After entering a pre-defined PIN code, other software levels
licensed to the authorised user may be accessed; This will enable the setting
and adjustment of additional instrument and test/reagent specific data or pa-
rameters.

Without access to the requisite PIN code, these software levels remain locked.
The next software level (expert level) has been set to default level 12345 by the
manufacturer. This may be amended at any time.

User Interface
The dialogue between analyzer and user takes place via the built-in touch screen.

! Note: Never use a sharp object

(finger nail, knife, pencil, let-
ter opener) to press on the control
The menus and menu items are selected by pressing the surface of the user con-
trol panel either by a fingertip or a blunt object, like a touch screen pen designed
for this purpose.
panel as it might cause irrepara-
ble damage to the control panel To operate the analyzer software different kind of menus, buttons, boxes and
surface. displays are available to select certain menus and functions, to enter informa-
tion and parameters and to display results, analyzer information and to view
graphics.

The following pages provide further information on available menus, software

areas, functions and defines the functionality of the analyzer software by using
the touch sensitive colour display.

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SYSTEM DESCRIPTION 27

2.2.1  Software control via touch screen

Figure 14
Main Menu functions

Displays associated functions combined in sub-men-

1- Area Frame
us or titles specific software areas.
Status Provides information about the current analyzer
2-
Information field status.
Are used to access additional menu/software areas,
3- Buttons
to confirm entry or to exit a screen.
Displays active or inactive functions, date & time
4- Status Bar information, individual messages, irregularities and
problems occurred during measurement.

Figure 15
Area frame, scroll buttons,
select window
 28

Displays associated functions combined in

1- Area Frame
sub-menus or titles specific software areas.
Are used to scroll up and down within a screen to
choose between certain available selections.
2- Scroll buttons Single arrows allow movement by one line
Double arrows allow movement by window/
page
Are used to access additional menu / software
3- Buttons
areas, to confirm entry or to exit a screen.
4- Select Window Displays available selections to choose.

Figure 16
Select box, Check Box

1- Check Box To set individual parameters On/Off press onto

the check box field.
OFF= not set 
ON = set 
2- Select Box Select between individual options by pressing
the arrow buttons.
3- Input Field To enter information using the virtual keyboard.
By pressing onto the white input fields a key-
board opens, allowing either alphanumeric or
only numeric entries.

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SYSTEM DESCRIPTION 29

Figure 17
Moveable Window

1- Moveable Information on windows not being displayed in total

Window may be moved by keeping the finger onto the screen and
then moving up/down and left/right. The window will
move according to the fingers direction.

2- Scroll buttons Are used to scroll up and down within a screen to choose
between certain available selections.
Single arrows allow movement by one line.
Double arrows allow movement by window/page.
3- Input Fields To enter information using the virtual keyboard. By
pressing onto the white input fields a keyboard opens,
allowing either alphanumeric or only numeric entries.
 30

Figure 18
Moveable Window (Graph)

1- Moveable Window Graphs of calibration and reaction curves not be-

(Graph) ing displayed in total on the screen may be
moved by keeping the finger onto the screen and
then moving up/down and left/right. The win-
dow will move according to the fingers direction.

Figure 19
Message Box

Provide information, Messages on Errors,

1- Message Box
Warnings and further available functions.

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SYSTEM DESCRIPTION 31

2.2.2  Software Overview

 32

2.2.3  Program Menus

This section gives an overview about the available software menus and software
areas to provide a quick guideline thought the overall analyzer software. See
User Manual for detailed info. Menu for an accurate description of the detailed
software functions.

2.2.3.1  Main Menu

The Main Menu automatically appears after the analyzer has been switched ON
and the initialization process of all analyzer modules has successfully been fin-
ished. Sometimes you must confirm a message, e.g. about the Shield Control
being disabled first.

Figure 20
Main Menu

The Main Menu is separated into two main sections

Menu Button Function
Within this area all submenus related to run preparation,
Run Preparation
incl. measurement, are located.
Within this area all submenus related to general analyzer
Setup Area setup, reagent and test definition, quality control and cali-
bration settings are located.
Shutdown Switching off the Analyzer or reboot the system.

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SYSTEM DESCRIPTION 33

Menu Button Function

By pressing into the input field a numerical keypad opens
allowing the entry of a PIN Code enabling access to other
Password/PIN
available software levels. Reset will reset the PIN Code to the
lowest available access level.
Selection of available rinsing, washing and cleaning cycles,
Rinsing Modes incl. Cleaning Cycle to clean the analyzer in regular intervals.

Within this area all submenus related to result organization

Results
and status information are located.
Submenus of the Main Menu are described in the following sections.

2.2.3.2  Run Preparation

Figure 21
Run Preparation

Menu Button Function

Load C-Ring Load of cuvette ring into the measuring rotor.
Displays the reagent load window, the positioning of rea-
Load Reagents gents and washers in the reagent area and selection of usa-
ble reagent/washer vials.
Displays the sample and STAT load window, positioning of
Load Samples
samples/STAT and selection of sample tubes usable.
Displays the controls load window, positioning of controls
Load Controls
and selection of control tubes usable.
Pressing the button measurement will allow access to the
Measurement
measurement menu.
The grey status information fields provide information about
Status Info the current status of cuvettes loaded, reagents prepared as
well as assigned samples and/or controls.
 34

2.2.3.3  Measurement

Figure 22
Menu Measurement

Menu Button Function

Provides information on the loaded reagents and
Reagent & Washer
washers, its required volumes, loaded volumes, sta-
Status List
bility and lot numbers.
Information about the cuvette ring, start cuvette, re-
Cuvettes quired, cuvettes to run measurement and available
cuvettes in total.
Information about the samples and/or controls to be
Samples+Controls
measured, fully and partially processed samples.
Tools Access to other functions, e.g. print a screen.
Opens the timeline window showing each step of the
Timeline joblist entered in a diagram and provides run time in-
formation.
Opens the joblist window showing the joblist of the
Joblist
next run.
Exit Return to MAIN MENU
Start the Run Button to initiate the measurement

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SYSTEM DESCRIPTION 35

2.2.3.4  Results
Results/Run
Figure 23
Results of last run

This window displays results of the last run in an abstract form only. This list
will be deleted as soon as new results are available out of a next run.
Menu Button Function
Press to send results to a HOST computer. Only possible if an
resend
active connection to a HOST PC is available.
Print To print short form result list via an external printer.
OK Return to MAIN MENU

Results/Memory
Figure 24
Result Selection

Menu Button Function

Sample ID Allows showing results available for an individual sample.
Test Allows showing results available for a specific test
Date Allows showing all available results for a selected date period.
Pressing this button will display all results selected or all re-
Show Results
sults available.
 36

Figure 25
Results/Memory

2.2.3.5  Status

Figure 26
Status Menu

Menu Button Function

Displays the analyzer status concerning temperatures of ro-
tor and pipettor, Container Status, cuvette ring information,
Status at…-
irregularities in the results measured, QC information as well
Information
as other errors appeared during measurement. Any problems
occurring may be marked coloured.
Print To print out the Status Screen
OK Return to MAIN MENU

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SYSTEM DESCRIPTION 37

2.2.3.6  Setup Area

Figure 27
Setup Area

Menu Button Function

Access the Setup Menu for setting analyzer, reagent and
Setup
testing specific parameters.
CuvCARD To load Cuvette ring balance from a CuvCARD to the analyzer.
QC Access the QC Menu for setting quality control parameters.
Perform calibration and management of calibration data,
Calibration
curves.

2.3  Measuring Principle

The analyzer uses a photo-optical detection principle. Clotting, chromogenic and
immunologic assays can be run simultaneously in random access, depending on
the available wavelength installed in the analyzer.

The measuring module consists of a two channel LED photometer, attached to

the measuring rotor.

-- 2 channel LED photometer with wavelengths at 405nm/570nm/740nm

 38

Figure 28
Measuring Principle

A light beam passes through the cuvette and is received by the photodiode. As
soon as the starting reagent is added to the plasma, the measuring time starts.

Any change in the light transmittance is detected and converted into an electri-
cal signal by the photodiode. The signal is converted into a digital value which is
transformed to the Extinction value.

When the assay is complete, the raw data (seconds for coagulation assays and
mE/sec (Extinction) for chromogenic assays) are processed and reported.

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INSTALLATION 39

3  INSTALLATION
This chapter provides instructions on how to unpack, install and prepare the
analyzer for use.
! Note: Before performing any
tasks in this chapter, review
the Hazard and Precautions listed
3.1  General Setup in chapter 1.4 of this manual.
Remove the analyzer from its packing and verify that the instrument has not
been damaged during transport and the accessories are complete, see Standard
Accessory List in Training Manual. Please notify your distributor or the manufac-
turer immediately in the event that the shipment was damaged or incomplete.
-- For the safety of the instrument and yourself the analyzer should be carried
or moved by two persons.
-- To carry the analyzer on the left and right bottom side of the analyzer.

Figure 29
Carry handles

-- Make sure a stable and level area for installation was selected and the instal-
lation area is free from dust, liquids, solvents, gas and acid vapors.
-- There should be free space of min. 15cm around the analyzer having free
access to the interface panel and power switch in the rear of the analyzer
! Never lift the analyzer on
the touchscreen arm this can
damage the instrument
and to install the liquid containers.
-- Lift the system from the box (with assistant), and place it on the installation
area.
-- Remove all foam inserts and set aside. ! Note: Pay attention to the pi-
pettor do not damage!

Be careful not to damage the pipettor, flat cable and steel tubing; any damaged
or defective parts must be replaced by a service technician before operating.
 40

! Note: The analyzer should be

placed in an area that allows
access to the main switch at all
-- Place the system in the correct position on the table.
-- Remove all tapes and transport protection devices. See Chapter 3.2.
-- Make sure the power cable will reach the outlet without using any extension
times. In case of any emergency, cords.
it may be necessary to immedi- -- There should be enough light for proper operation, but no direct sunlight
ately switch off the analyzer. and artificial light, e.g. lamps to avoid premature formation of algae in the
tubing system.

! Note: The analyzer power cord

must comply with the nation-
al standards in the country the
-- The ambient temperature must range from +15°C to a maximum of + 30°C.
Avoid significant temperature changes, humidity and voltage variations, see
Technical Specification Flyer.
equipment is supplied.

3.2  Removing the Transport Protections

! Note: Do not damage any

packaging material, and keep
them for possible later use, in-
The analyzer is secured with several transport protection parts to avoid any
damage of the analyzer or components during its transportation.

cluding the transport protection Prior of use the analyzer has to be released from any of these transport protec-
devices. Store them away from tion devices.
heat and humidity.
Transport protection devices are:

1. Cardboard insert inside transfer arm: Protecting the pipettor from moving in
Y-direction.

Figure 30

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INSTALLATION 41

2. Cardboard insert backside: Protecting the pipettor transfer arm from mov-
ing in X-direction.

Figure 31

3. Red plastic plate with cable ties: Protects the pipettor from moving in Z-di-
rection.

Figure 32
 42

4. Empty red cuvette ring in the measuring rotor to protect from contamina-
tion of the measuring cells.
Figure 33

3.3  Assembly of the analyzer

! Note: Before connecting the

analyzer to the main power
supply, complete all instructions 3.3.1  Connect the container sensors
in this section. The analyzer is equipped with two water containers (distilled water and waste
water). All containers should be located to the left side, behind the analyzer. The

! Note: The system must be

switched OFF.
distilled water temperature may not exceed the specified temperature of the
analyzer (+15°C to + 30°C).
-- Place the distilled water container (with the green label) and the empty

! Note: The system will only

function if the distilled and
waste water tubes have been
waste water container (with the red label) to the left rear of the analyzer. Fill
the distilled water container with at least 1 litre of distilled water.
-- Place the colour-coded sensors into the appropriate containers (green for
connected properly to the system dist. water, red for waste water).
and the tube for the dist. water is -- Plug the green and red colour-coded couplings into their matching sockets
completely filled without air bub- on the side of the system (see Figure 34). A „click“ indicates the proper seat-
bles. ing of the connectors.
-- Connect both sensor plug-in connectors to the analyzer and turn the move-
able ring of each connector clockwise to lock them.

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INSTALLATION 43

Figure 34
Connect the level sensor
tubes to the analyzer

! Note: The distilled water container must have at least 1 litre of distilled wa-
ter. The waste water container should be emptied every day. Be careful not
to have air bubbles in the distilled water container as well as in the tube. The
! Note: DI water need to be
placed on the same Table
Level like the analyzer for proper
distilled water container may not be placed in direct sunlight. work.

Container

Figure 35
Container for distilled water. Con-
tainer and tubes are coded green.

Figure 36
Container for waste water.
Container and tubes
are coded red.
 44

3.3.2  Connect an USB Memory Device

The analyzer is equipped with two USB interfaces. This two USB interfaces are
located at the rear side of the analyzer utilized for connecting an external print-
ing device or USB-Stick.

Figure 37
USB Interface

The USB interface offers the option to connect a USB memory device to record
and save measuring data, i.e. results, reaction curve and patient data and to
import and export test and reagent specific data using a commercially availa-
ble USB stick. These data can be viewed later on the display of the analyzer. To
record measuring data first insert a USB Stick into the USB interface see figure
above. Leave the USB Stick in the interface, the USB Stick will be mounted once
the analyzer is switched on.
The USB Stick should have a memory of at least 1GB up to 32GB and has to be
formatted with the file system FAT only. Other file systems as NFTS or others are
not accepted by the analyzer.

3.3.3  Connect an external printer

The analyzer offers the possibility for connecting an external USB printer device
using the USB interfaces (top position) at the rear side of the analyzer; this al-
lows print put of results, reaction curves, instrument- and test parameters.
-- To connect the USB printer device use the USB cable provided in the accesso-
ries of the printer. Use the topmost USB interface in the rear of the analyzer
to connect the printer to the analyzer.
-- Connect the power supply plug to the printer.
-- The analyzer will automatically detect the USB printer once connected to
the analyzer. To verify the connection is set correctly the symbol print in the
status line of the analyzer must turn green.

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INSTALLATION 45

Recommended Printers
The following printers have been tested, but all PCL6 / PCL3 compatible printers
are likely to work.
-- HP Officejet 6000
-- HP Officejet Pro 6230
-- HP Deskjet 6940
-- HP Deskjet 6930
-- HP Officejet Pro 8500
-- HP Officejet Pro 8100
-- OKI C332
-- Kyocera ECOSYS P5021cdn
-- Ricos SP252DN
-- OKI C332
-- Kyocera ECOSYS P5021cdn
-- Ricos SP252DN
-- HP LaserJet Pro M203dn

3.3.4  Connect an external Barcode Scanner (option)

-- Connect the optional barcode scanner to the analyzer. Use the recommend-
ed type only.
-- Connect the barcode scanner data cable to the RS232C socket (D-Sub) on the
analyzer (Figure 14).
 46

3.3.5  Protection with power safety socket (surge protector) and

online UPS
With immediate effect all instruments of HUMAN’s Elisys, HumaStar, Huma-
Count and HumaCount Pro Lines will be shipped with the following additional
accessories. This will be done without any additional charges.

18969 Multiple power safety socket

18968 Adapter EU to UK
18967 Adapter EU to USA

The following information gives an overview of the basic principles and the in-
stallation of the additional accessories.
However this applies also for all other instruments, where an Online UPS and
the above mentioned parts can be ordered separately.

3.3.5.1  Installation
1. Plug-in the multiple socket incl. surge protector to the wall socket.
2. Power on the switch and check whether the green and the red LED are turned
on.
This indicates a correct grounding and the correct protection circuit.

Figure 38

3. Connect the UPS to one of the surge protector sockets.

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INSTALLATION 47

Figure 39

Figure 40
Control panel

4. The UPS switches on and the Bypass-LED, Figure 41

the Line-LED and the LED of the battery UPS in Bypass mode
status monitors that the UPS is in pow-
er-on mode.
5. The system still runs in Bypass mode. That
means, that the battery is not available
and the Consumers are not protected.
6. To assure a proper protection for all con-
sumers, the system has to be switched to
‘normal’ Mode.
 48

Figure 42 7. Keep the ‘ON-button’ pressed for at least

UPS Control Pnael 8 sec. until all LEDs switch on for a moment.

8. After that, the LEDs turn on and off one by

one. At the end the ‘Bypass-LED’ must be off
Figure 43 and the ‘Inverter-LED’ must be on.
UPS in Normal mode

To ensure the UPS provides maximum power in case of a mains power inter-
ruption, the UPS has to be fully charged before consumer devices should be
plugged-in.
The battery charging process may take 1 -2 hours. 5 LEDs will monitor the cur-
rent status of charging.

! Note: Never connect a printer to the UPS. The UPS might be damaged due to
a high pull-in current of the printer. Use the multiple socket with the surge
protector included.

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INSTALLATION 49

3.3.5.2  Ground circuit check

! Note! The following instruction should only performed by a professional

electrician.

1. First check, that the electrical socket is grounded. For that use a multimeter
(available in your electronics market).

Figure 44
European wall socket

Neutral

Earth/Ground

Phase

! Please note, that this is just an example. The neutral conductor and phase
conductor might be connected of course also the other way around!

2. Set the multimeter knob to AC and connect the black probe into neutral and
the red probe into phase as shown in figure below.

Figure 45
Neutral across Phase

3. The voltage across Neutral and Phase reads 225V.

4. Insert the black probe in earth / ground and leave the red probe connected
to phase.
 50

Figure 46
Phase across Ground

5. It reads 224V.
6. Next, read the voltage across earth / ground and neutral conductor. For that
insert the black probe in neutral and the red probe in earth / ground.

Figure 47
Neutral across Ground

7. It should not read more than 2V.This example reads a voltage of 0,597V.
8. Calculate the voltage difference between Step 5 and Step 3 i.e., 224V - 225V
= -1V.
9. Calculate the voltage difference between Step 8 and Step 7 i.e., -1V – 0,597V
= -1,597V. (+/-1 to -/+2V is an acceptable value).

! This is just to test if a grounding circuit exists. In order to measure the actual
grounding resistance, you would need an Earth Resistance Tester.

3.3.5.3  Power Outage

A power outage (also power cut, blackout, or power failure) is a short- or long-
term loss of the electric power to an area.
There are many causes of power failures in an electricity network. Examples of
these causes include faults at power stations, damage to electric transmission
lines, substations or other parts of the distribution system, a short circuit, or the
overloading of electricity mains.

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INSTALLATION 51

Power failures are particularly critical at sites where the environment and public
safety are at risk like hospitals and laboratories.

3.3.5.4  Surge Protector (5 sockets)

A surge protector is an appliance designed to protect electrical devices from
voltage peaks. A surge protector attempts to limit the voltage supplied to an
electric device by either blocking or by shorting to ground any unwanted voltag-
es above a safe threshold.
The multiple socket below has a built-in surge protection. It monitors correct
grounding of the wall socket and correct work of protection circuit.

Figure 48
Multiple socket incl. surge
protector (part no. 18969)

3.3.5.5  Ground
Poor grounding not only contributes to unnecessary downtime, but a lack of
good grounding is also dangerous and increases the risk of equipment failure.
Without an effective grounding system, we could be exposed to the risk of elec-
tric shock, not to mention instrumentation errors, harmonic distortion issues,
power factor problems and a host of possible intermittent dilemmas. If fault
currents have no path to the ground through a properly designed and main-
tained grounding system, they will find unintended paths that could include
people.
However, good grounding isn’t only for safety; it is also used to prevent damage
to equipment.
A good grounding system will improve the reliability of equipment and reduce
the likelihood of damage due to lightning or fault currents.
Ground terminal can become a noise source as well, if system ground is not
good enough. It is recommended to have a voltage lower than 1V.
 52

3.3.5.6  Online UPS (Uninterruptable Power System)

The online UPS is ideal for environments where electrical isolation is necessary
or for equipment that is very sensitive to power fluctuations. The online UPS
may be necessary when the power environment is “noisy”, when utility power
sags, outages and other anomalies are frequent, when protection of sensitive IT
equipment loads is required, or when operation from an extended-run backup
generator is necessary.

3.3.5.7  Function
The basic technology of the online UPS is the same as in a standby or line-in-
teractive UPS. However it typically costs much more, due to it having a much
greater current AC-to-DC battery-charger/rectifier, and with the rectifier and in-
verter designed to run continuously with improved cooling systems. It is called
a double-conversion UPS due to the rectifier directly driving the inverter, even
when powered from normal AC current.

In an online UPS, the batteries are always connected to the inverter, so that no
power transfer switches are necessary. When power loss occurs, the rectifier
simply drops out of the circuit and the batteries keep the power steady and un-
changed. When power is restored, the rectifier resumes carrying most of the
load and begins charging the batteries, though the charging current may be
limited to prevent the high-power rectifier from overheating the batteries and
boiling off the electrolyte.
The main advantage to the on-line UPS is its ability to provide an electrical fire-
wall between the incoming utility power and sensitive electronic equipment.

3.3.5.8  Purpose of using a UPS and Surge Protector

To protect Human instruments as well as Computer systems and other elec-
tronic devices, the use of an uninterruptible power supply (UPS) can provide a
constant flow of electricity in the event that a primary power supply becomes
unavailable for a short period of time.
To protect against surges (events where voltages increase for a few seconds),
which can damage hardware when power is restored, a Surge Protector that ab-
sorbs the excess voltage and a UPS will be included to future instruments orders
excepting the small equipments as HumaLyzer line, HumaLyte line and smaller.

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INSTALLATION 53

3.3.5.9  Power socket adapter

Depend to your local socket-outlet, an additional adapter might be required. The
following adapter are suitable for all earthed and unearthed 2- & 3-pole devices.

Figure 49 Figure 50 Figure 51

UK adapter (part no. 18968) Front side of both adapter UK adapter (part no. 18968)
 54

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Repacking and decontamination 55

4  REPACKING AND DECONTAMINATION

! Note: Please clean and disinfect the analyzer according our decontamination
instruction before transport!

4.1  Repacking

4.1.1  Transport Safety

4.1.1.1  Disconnect the Level Sensors

1. Disconnect Waste Water cable Figure 52

2. Disconnect DI Water cable
3.
4.
Disconnect Tubing Waste Water
Disconnect Tubing DI Water 1 2
-- Immediately eliminate leaking
liquids.

3 4

4.1.1.2  Remove all loose parts from Instrument

1. Remove all CupDisconnect DI Figure 53

Water cable
2. Remove Cuvette Ring, incl. Mixer 3 2
3. Remove all adapter and vials
4. Remove reagent rack

No loose parts may remain inside

the Instrument .

4 1
 56

4.1.1.3  Secure Measuring Rotor

Figure 54 1. Insert empty Cuvette Ring into

the measuring rotor.

1 2. Secure Cuvette Ring with tape.

-- Remove all Mixers from Cuvette

Ring.
-- Do not use a damaged Cuvette
Ring.

4.1.1.4  Z-Protection

Figure 55 1. Place the Z-Protection behind

the pipettor (1).
2. Secure using the tyraps (2).

1
2 -- Pipettor may not be moved in
Z-direction.

4.1.1.5  Prepare Y-Protection

Figure 56 1. Insert the Y-Protection in the

way as shown in picture (1).
2. Secure by pressing the strips up-
2 wards (2x).
3. Secure by pressing the strips
downwards (2x)
-- Pipettor may not move in Y-di-
rection.

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Repacking and decontamination 57

4.1.1.6  X-Protection

1. Insert the X-Protection in the Figure 57

way as shown in picture.
2. Secure by pressing the slips
inside.

Transfer arm may not be moved in

X-direction.

4.1.2  Packaging

4.1.2.1  Insert foam inserts

1. Insert foam insert 1.

2. Insert foam insert 2. 1 2 Figure 58
 58

4.1.2.2  Instrument

1 1. Place Instrument into the foam

Figure 59 parts.

-- Make sure the Instrument carries

all transport protections.
-- No loose parts may remain in the
Instrument.

4.1.2.3  Plastic Cover

Figure 60 1 1. Cover instrument with plastic

cover.

4.1.2.4  Foam Inserts Top 1

Figure 61 1. Foam Insert Top 1.

1

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Repacking and decontamination 59

4.1.2.5  Standard accessories

1. Insert Standard accessories. Figure 62

4.1.2.6  Foam Inserts Top 2

1. Place Foam Insert Top 2 as Figure 63

shown in picture.

4.1.2.7  Shipping Box

1. Place Foam Insert Top 2 as Figure 64

shown in picture.
 60

4.2  Decontamination and prepare for shipment

Decontamination OK?

Instrument decontaminated [YES] / [NO]

(wipe all surfaces of the instrument with alcohol)
[YES] / [NO]
Certificate of Decontamination added

Prepare for Shipment

Fill DI Water with minimum 1l [YES] / [NO]

Place Washer to W1 [YES] / [NO]

Place Cleaner to W2 [YES] / [NO]

Run Intensive 1 Washing Cycle [YES] / [NO]

Run Cleaning Cycle [YES] / [NO]

Run Flush 10 second 3 times [YES] / [NO]

Pull DI sensor out of container [YES] / [NO]

Remove Filter of DI Sensor [YES] / [NO]

Run Flush 10 second 3 times to empty water out of

[YES] / [NO]
pumps and pipes

Wipe outside Pipettor Probe with alcohol [YES] / [NO]

Use pressure air to clean Sensor (waste & DI Water) [YES] / [NO]

Empty Containers clean with alcohol and dry containers [YES] / [NO]

Uninstallation Procedure OK?

Install all Transport Protection (rotor, Pipettor Arm X,Y,Z) [YES] / [NO]

Take out Reagent Rack [YES] / [NO]

Add all according Packaging list [YES] / [NO]

(except of waste container & tubing)

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Service Menu 61

5  SERVICE MENU

5.1  Accessing the Service Software

Every time at Software start the selection to start the SERVICE software is possi-
ble for 5 seconds by clicking at the touchscreen for min 3 times. In this case first
the possibility to calibrate the touch is provided. After a timeout of 5 seconds
the service software is started. The operator must enter the correct password
for the levels SERVICE or FACTORY. In case the correct password is entered the
service software is started. In case the wrong password is entered the user soft-
ware is started automatically.

If during software start the touch is not selected three times then after 5 sec-
onds the normal menu for the USER software is started automatically.

It is also possible to start the Service Software from the USER Software. The SER-
VICE button is available only if password level SERVICE is active. It will access the
menu structure shown below.

Figure 65
 62

5.2  Report Section

If the user first enters a selftest function the report file is named selftest_id.txt,
whereby id is a unique time coded alphanumeric string to prevent the system
overwriting older protocol files.

The file is memorized to USB-Stick (if inserted) or to the host drive (if connected).
If neither host nor USB-Stick is available no report file is memorized.

5.2.1  Button SHOW

Select this button to display the currently available service.txt or selftest.txt file.
The report can be printed and closed.

Figure 66

5.3  Data Section

5.3.1  Button USB-STICK

Figure 67

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Service Menu 63

5.3.2  Button PARAMETERS

The parameters of the analyzer are used for the storage of device specific posi-
tions, temperature target values or optics calibration values as well as test and
reagent parameters. Selecting the button PARAMETERS will open the following
dialogue:

Figure 68

Selecting the button LOAD DEFAULTS will reload the device specific DEFAULTS
parameters and then performs reset. Before this action is done, a security ques-
tion and possibility to cancel is provided.

The button IMPORT USB will import parameters from a text file in the USB-Stick
root directory. Remark: The USB-stick must be attached to one of the analyzer
USB-Host ports and must be mounted.

The button EXPORT USB will export parameters from to a text file in the USB-
Stick root directory.
Selecting the button IMPORT LAN will import parameters from a text file in the
LAN analyzer directory. The name of the parameter text file must start with
“par”.

The button EXPORT LAN initiates an export of all parameters as a text file into
the LAN analyzer directory.
Select the button QUIT button to leave this menu.

Remarks: Import of parameters will ignore the system specific plausibility tests
in the setup menus. Importing of invalid parameters may damage components
and can lead to malfunction of the system. In this case select LOAD DEFAULTS to
ensure proper function!
 64

5.3.3  Button SOFTWARE

Software updates starting from software below V1.2 please request the security
code from Human Technical Service before starting the update process.
Software updates starting from software V1.2 or higher, the security code could
be re-used. Therefore please note down the security code from the report sec-
tion before you start with the software update.

A Software update of the analyzer can be done in two different ways: By use of
LAN connection or by use of the USB-Stick. In both cases the Software update
file must be located in the target directory.
Figure 69

Button USB-STICK: If selected, all available software packets in the root directory
of the USB-Stick will be displayed and the user can select one file. After selection
the Software update is performed. During the Software update the parameters
of the analyzer are exported and after the update took place, imported. The
USB-Stick must be mounted to the analyzer during the software update.

Button LAN: If selected, all available software packets in the LAN directory will
be displayed and the user can select one file. After selection the Software up-
date is performed. During the Software update the parameters of the analyzer
are exported and after the update took place, imported.
Select the button QUIT to close the dialogue without modification.

5.3.4  Button SERIAL NUMBERS

Input fields for the following serial numbers are displayed:
ANALYZER
POWER-SUPPLY
ROTOR
PIPETTOR
SCANNER
PUMPS
STIRRER_DISTRI
MAINBOARD
DILUTOR
PHOTOMETER
LCD DISPLAY
COVER LOCK
XYZ DISTRI

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Service Menu 65

20 alphanumeric characters can be entered into each edit filed.

Selecting the button TOOLS displays the buttons QUIT and HARDCOPY. Quit will
close the dialogue without modification, HARDCOPY will perform a printout of
the SERIALNUMBERS screen by use of a connected USB printer.
Selecting the button UNDO&EXIT will close the screen and discard all modifi-
cations.
Selecting SAVE&EXIT will close the screen and save all modifications.

5.3.5  Date and Time

In this menu it´s possible to change the Date and Time and the Format for Date
and Time.

Figure 70
 66

5.3.6  Access Mode

Here you could choose between the different Assey protection modes of the HC
Pro.

Figure 71

! Note: Do not change the Ac-

cess Mode in the Service menu
unless you have a valid security
key. Without a valid security key,
the system will not be able to per-
form measurements. Security Key

Figure 72

Systems with SN higher K25900147 will be sold in Restricted Mode. The Unre-
stricted Mode for systems with SN higher K25900147 will be available by pur-
chasing a key.
If you would like to change to unrestricted mode please create via your
MyHuman account a call type “key request”.

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Service Menu 67

5.4  Electronic Section

5.4.1  Button PWR. SUPPLY

The power supply of the analyzer provides +24 Volt DC. This voltage is the power
supply of the mainboard. All sub-voltages are generated on the mainboard by
use of DC/DC converters. Each converter is equipped with a separate fuse. The
state of all fuses can be monitored on the analyzer LCD. Also the state of the
temperature regulated fan for the power supply can be monitored.

Figure 73

One section displays the status of the 24 V voltages for all 12 fuses, located on
the analyzers main board, updated periodically.

In the section FAN the pwm value as well as the rpm value for the fan speed is
displayed.
One display of the TEMPERATURE SENSOR value inside the power supply hous-
ing. The target value for the power supply is +40°C.
Selecting the button SELFTEST will perform the power supply fan test with dif-
ferent speeds.

The button TOOLS displays a new dialogue with the option to print this screen.

The button OK will close this screen.

 68

5.4.2  Button PHOTOMETER

Functional principle

Measuring circuit
Figure 74
Analog /
Trans-
Light to Current to Digital
SMD LED Cuvette Aplifier mission ->
Current Voltage Conversio
Extinktion
n

Reference circuit
Analog /
Light to Current to Digital
Aplifier
Current Voltage Conversio
n

The photometer is equipped with 2 channels. Each channel is equipped with a

reference. For each channel 3 wavelengths are provided. 405nm, 570nm and
740nm are available. A 16 stage amplifier is available for each channel and ref-
erence channel to ensure high dynamic ranges for the AD conversion. A stepper
motor is provided for each channel, the stir bar inside the cuvette is turned by
the magnetic head of the stepper motor. The photometer unit firmware can be
updated during the software update of the analyzer.

Figure 75

The PHOTOMETER screen consists of 4 sections: CHANNEL 1, CHANNEL 2, COM-

MON and GENERAL.

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Service Menu 69

Section CHANNEL 1:
Display of value for MEAS [digits] periodically updates and shows the current
value for the measuring channel 1.
Display of value for REF [digits] periodically updates and shows the current
value for the reference channel 1.
Display of value for TRANSMISSION [%] periodically updates and shows the
current transmission for channel 1.
Display of value for ABSORBANCE [mE] periodically updates and shows the
current transmission for channel 1.
GAIN MEAS shows the currently selected amplification [0…15] stage for the
measuring channel 1.
GAIN REF shows the currently selected amplification [0…15] stage for the refer-
ence channel 1.
Edit field TARGET RPM allows entering of the RPM target value for mixer 1
[200…999].
Display field MIXER RPM periodically updates and shows the current RPM for
mixer 1.
Info checkbox for RPM ERROR is checked if the currently calculated RPM value is
out of the allowed thresholds.

Section CHANNEL 2:
Display of value for MEAS [digits] periodically updates and shows the current
value for the measuring channel 2.
Display of value for REF [digits] periodically updates and shows the current val-
ue for the reference channel 2.
Display of value for TRANSMISSION [%] periodically updates and shows the
current transmission for channel 2.
Display of value for ABSORBANCE [mE] periodically updates and shows the
current transmission for channel 2.
GAIN MEAS shows the currently selected amplification [0…15] stage for the
measuring channel 2.
GAIN REF shows the currently selected amplification [0…15] stage for the refer-
ence channel 2.
Edit field TARGET RPM allows entering of the RPM target value for mixer 2
[200…999].
Display field MIXER RPM periodically updates and shows the current RPM for
mixer 2.
Info checkbox for RPM ERROR is checked if the currently calculated RPM value is
out of the allowed thresholds.
 70

Section COMMON
Checkbox LIGHTS ON: If checked the selected LEDs are powered on Selectbox
WAVELENGTH enables the selection between the three available wavelengths
(405 nm, 570nm, 740nm).
Display field STATUS displays the state of the photometer unit [OK or ERROR].

Section GENERAL
The button INFO displays the parameter values for the photometer calibration
of each wavelength. The screen can be printed.

Figure 76

The button CALIBRATE displays the parameter values for the photometer cali-
bration of each wavelength. The screen can be printed.

Figure 77

Selecting NO closes the dialogue and displays the PHOTOMETER main screen
Selecting YES displays the following selection REPLACED /UPDATED.

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Service Menu 71

Figure 78

If the button REPLACED is selected, all photometer parameters are overwritten

during the following calibration routine.
If the button UPDATED is selected, all photometer parameters are overwritten
during the following calibration routine, except for the parameters of the refer-
ence brightness storage.
If no selection is done within 10 seconds, automatically the calibration is per-
formed with the option UPDATED.
Then the automatic sequence for calibration is performed as follows.
-- Power on the LED pairs for 405nm and wait for 5 seconds (thermal balance)
-- Select gain 0 for all 4 channels and perform ADC conversions, check values
are <7400 counts.
-- Increase gain up to 15 step by step, check ADC values are <7400 counts.
-- Memorize the gain value for each channel.
-- Repeat the procedure for 570nm.
-- Repeat the procedure for 740nm.
-- Select the gain settings for 405nm and perform bright value measurements
on all channels.
-- Select the gain settings for 570nm and perform bright value measurements
on all channels.
-- Select the gain settings for 740nm and perform bright value measurements
on all channels.
-- Dark values are 0 for all channels and all wavelength because of ambient
light compensation.

Figure 79

The button TOOL enables to print the page by use of the button HARDCOPY, to
quit this dialogue or to start a MIXERTEST.
Button TOOLS\MIXERTEST: The mixer motors are controlled alternating with
the following RPM values:
100, 300, 500, 700, 900, 1100 RPM
The software checks is the target RPM values are performed correctly. At the
end of the procedure the test result OK or ERROR is displayed.
 72

5.4.3  Button CHIPCARD

When selecting this button, the information stored on the CuvCard are dis-
played.
Figure 80

If no CUVCARD is present a corresponding message is shown.

Select the button PRINT to print out the data of the Chip Card.
Enter the button EXIT to leave this menu.

5.4.4  Button BARCODE SCANNER

Figure 81

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Service Menu 73

The Info Checkbox displays if the analyzer is equipped with an internal or exter-
nal scanner.
Button GET INFO: Selection displays the firmware information of the MS-1 bar-
code scanner.
Select the button SCAN to scan a barcode, 10 sec timeout. The scanned label is
displayed in the field below.

The button MS-1 setup performs the following initialization sequence:

Figure 82

<K140,0>; // point to point without rts/cts

<K141,0>; // no Preamble
<K142,1,\r> // CR as Postamble
<K222,1>; // read only 1 barcode per read cycle
<K503,2550> // after min 2,55sec display of same label
<K200,2>; // read only, when object is appearing
<K221,2>; // each barcode is read 2 times
<K470,1,0,0,0,0,0,0> // Code39
<K471,1,0,0,0,0,6,0,0> // Codabar min. 6 digits
<K472,1,0,0,6,6> // 2of5 ITF min. 6 digits
<K474,1,0,0,1,1,0, > // Code 128/EAN128
<K488,1,0,0,0,6,6> // 2of5 Standard min. 6 digits
<K489,1,1,1,1,0,0,0,1,0,0> // UPCA -> EAN13
<K489,1,1,1,1,0,0,0,0,0,0> // UPCA -> UPCA

Select the button TOOLS and then PRINT to print out the current screen.
Select the button UNDO&EXIT to discard all changes and close the screen with-
out modifications.
Enter the Button EXIT to close the dialogue.
 74

5.4.5  Button CONTAINER SENSORS

Figure 83

Functional principle
ADC Values higher than the threshold value will be interpreted as FULL, values
below the threshold are interpreted as EMPTY. The HYSTERESYS value is used to
avoid permanent changes in the states FULL/EMPTY once the liquid level inside
the container in exact at the level of the liquid sensor of the external level sensor.

Section SYSTEM-WATER
Display of ACTUAL DISITS value for the system water sensor
Edit field for the THRESHOLD value of the system water sensor
Edit field for the HYSTERESIS value of the system water sensor
Display field for the STATE of the system water sensor [FULL/EMPTY]

Section WASTE-WATER
Display of ACTUAL DISITS value for the waste water sensor
Edit field for the THRESHOLD value of the waste water sensor
Edit field for the HYSTERESIS value of the waste water sensor
Display field for the STATE of the waste water sensor [FULL/EMPTY]

The Button TOOLS will display the buttons PRINT to hardcopy the screen and the
button EXIT to leave the dialogue
The Button UNDO&EXIT will discard all changes and leave the screen
The Button SAVE&EXIT will memorize all changes and exit the screen

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Service Menu 75

How to calibrate the sensors

Prepare 30°C warm distilled water.

Remove the Liquid sensor from the water and wait until you have a “nearly” sta-
ble “actual” value. Note this value.

Calculate the new threshold value.

5.4.6  Button DISPLAY

Figure 84

Use the buttons BRIGHTER to increases the brightness value for 10%, the button
DARKER decreases the brightness value for 10%.

The Buttons MINIMUM and MAXIMUM change the brightness to 20% or 100%.
The currently selected set value is displayed in the top left corner.

Select the button QUIT to leave the screen and memorize the settings.

5.4.7  Button CALIBRATE TOUCH

Once the button is pressed, the following screen is displayed.

Figure 85

If OK is selected a dialogue is shown indicating that the following calibration

routine requires touching into 2 circles or do keep the touch calibration by not
touching the screen during the calibration procedure.
 76

The screen is automatically closed after calibration is performed.

Figure 86

Figure 87

HumaClot Pro | Service Manual

Service Menu 77

5.5  MECHANICS Section

5.5.1  Button TEACH-IN

Selecting this button will initialize the XYZ and Measuring rotor. A new screen
with a schematic representation of all TEACH-IN positions for the red XYZ ad-
justment tools is displayed. The red positions are ACTIVE buttons.

Figure 88

Button TOOLS opens a dialogue with the button HARDCOPY to print out the
screen and QUIT to close the TOOLS screen.

Figure 89
 78

Symbol for RINSING STATION:

If selected a selection screen with 3 options QUIT, GOTO and TEACH-IN is dis-
played.
Button QUIT will leave the dialogue without action.
Button GOTO will move the XYZ arm the XY position of the rinsing station
whereby z remains in top position (zero).
Button TEACH-IN will power off the XYZ servo motors so the arm can be moved
manually. The position values for X,Y and Z are displayed. The user must insert
the adjustment tool into the rinsing station and move the Pipettor tip manually
into the target drill hole of the tool.
Selecting the button CANCEL will power on all servo motors and move the Pipet-
tor to top position.
Selecting SAVE will memorize the new Z-QUICK position for the rinsing station,
power on the servo motors and move the Pipettor to the z top position.

Symbol for REAGENT POSITION 1 /15:

If selected a selection screen with 3 options QUIT, GOTO and TEACH-IN is dis-
played.
Button QUIT will leave the dialogue without action.
Button GOTO will move the XYZ arm the XY position of the reagent position 1
whereby z remains in top position (zero).
Button TEACH-IN will power off the XYZ servo motors so the arm can be moved
manually. The position values for X,Y and Z are displayed. The user must insert
the adjustment tool into the reagent position 1 and 15 and move the Pipettor
tip manually into the target drill hole of the tool which is in position R1.
Selecting the button CANCEL will power on all servo motors and move the Pipet-
tor to top position.
Selecting SAVE will memorize the new Z-QUICK position for the reagent position
R1, power on the servo motors and move the Pipettor to the z top position.
Again the selection screen with 3 options QUIT, GOTO and TEACH-IN is displayed.
Button QUIT will leave the dialogue without action.
Button GOTO will move the XYZ arm the XY position of the reagent position 1
whereby z remains in top position (zero).
Button TEACH-IN will power off the XYZ servo motors so the arm can be moved
manually. The position values for X,Y and Z are displayed. The user must move
the arm to the target position into the drill hole of the adjustment tool in R15.
Next the software calculates the positions R2 to R 14 based on an internal soft-
ware data matrix. If all positions are within specific maximum ranges the pa-
rameters are valid and will be memorized. A corresponding info screen is dis-
played.

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Service Menu 79

If the calculated position values are out of the allowed ranges an different mes-
sage is displayed and the parameters are not memorized. The original parame-
ter values remain active and changes are rejected.

Symbol for WASHER 1 / CONTROL 8 POSITION:

The procedure is like described with the following exceptions;
-- The adjustment tools have to be placed into positions W1 and Control 8.
-- The positions for W2 and C1…C7 are calculated by software.

Symbol for CUVETTE 1/17 POSITION:

The procedure is like described with the following exceptions;
-- The adjustment tool has to be placed first into positions CUV 1 and next into
position CUV 17.
-- The positions for CUV2…CUV15 and CUV 18…CUV 32 are calculated by Soft-
ware.

A vertical line on the measuring rotor plates is displayed for the cuvettes, which
shows the exact positions of the tools in the measuring rotor.

Figure 90

Symbol for SAMPLE 1/25 POSITION:

The procedure is like described with the following exceptions;
-- The adjustment tool has to be placed first into position S1 and next into
position S25.
The positions for S2 …S24 are calculated by software.

1 Button SHOW ALL POSITIONS:

All positions of the analyzer are displayed on the screen. By use of a PRINT but-
ton the positions can be printed. The OK button will close the screen.
 80

1 Button GOTO ALL positions:

A selection to stop at each position [YES/NO] must be selected. If YES is selected,
the user must press a button to initiate the movement to the next position.
Next a selection to move to [TOP/QUICK] position must be done.
At least the target area must be selected.

-- WASHER will check the 2 washer positions

-- CONTROL will check the 8 control positions
-- CUVETTE will check the 32 cuvette positions
-- RINSING will check the rinsing station position
-- REAGENT will check all reagent positions of the reagent tray
-- SAMPLE will check all 25 sample positions

5.5.2  Button STIRRER

Figure 91

Section ROTATION:
Display of the current RPM values for the reagent stirrers 1 to 5
Display of the target RPM value (parameter value)
Display of REVERSE/SEC for the Time after the rotation direction changes
Display of ON/SEC for the on cycle time
Display of OFF/SEC of the off cycle time
Five checkboxes to select the participants for the Stirrer test (default = 1, 2, 3
checked).

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Service Menu 81

Display of PWM value for stepper motor chopper frequency

Display of global RPM value which is valid for all 3 motors

Button SELFTEST. This performs an subsequent rotation of stirrers 1, 2, 3 with

200 rpm and then 400 rpm. If the test is passed correctly a corresponding info
screen is displayed at the end of the test procedure. If the test is failed the user
can see detailed information about the mixer number and rpm target value that
failed the test.

Button TOOLS opens a dialogue with the button HARDCOPY to print out the
screen and QUIT to close the TOOLS screen.
Button OK leaves the screen.

5.5.3  Button ROTOR

Figure 92

Section TEMPERATURE REGULATION

Display field of the TARGET temperature [35…42°C]
Edit field for TARGET ADC digits
Display field for ACTUAL measured digits [15…50°C]
Display field for DIFFERENCE of digits (TARGET-ACTUAL)
Display field for PWM [0…77,5%] Remark: limited to 77,5 % because heating and
temperature measuring are performed in time multiplex.
Display of check box for STABLE. State STABEL is checked if ACTUAL digits are
TARGET digits ± 20 digits
Check box for REGULATION ON. If checked, the temperature regulation is acti-
vated.
 82

5.5.4  Button CALIBRATE

If selected the numeric software keyboard is displayed and the user must enter
the TARGET temperature in °C, confirm with ENTER.
Next the numeric software keyboard is displayed again and the user must en-
ter the ACTUAL measured temperature in °C, confirm with ENTER. Remark: The
Rotor temperature is measured by use of an external temperature measuring
device.
The software calculates the new TARGET digits value based on the TARGET and
ACTUAL temperature difference and an internal correlation function for the Pi-
pettor temperature. Target temperature is 38.5.

Section Rotation
Button Stresstest:
If selected the rotor is initialized. Next the rotor moves to stochastic positions
until the STRESSTEST button is pressed again.

5.5.5  Button CHECK Ring

A message is displayed with a decision if the photometer measures transmis-
sion values for all 32 cuvette positions. Remark: Photometer must be available
and correctly calibrated. Selection YES will move the cuvettes 1 and 2 into the
optical path and measure the transmission value. A XY graph is displayed with
the cuvette number on the X axis and the Transmission [%] on the Y axis.

Figure 93

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Service Menu 83

5.5.6  Button TEACH-IN

Figure 94

If selected, a dialogue box is displayed with the decision TEACH IN ROTOR MAN-
UALLY? If YES is selected, the rotor servo motor is powered off and the rotor
must be turned that cuvette 1 and 2 are centred in the optical path. A specific
pin can be used for this procedure. The Message must be confirmed with OK.
Next a dialogue box is displayed with the decision FURTHER OPTIMIZE BY USE
OF PHOTOMETER? If YES is selected a XY graph is displayed with the cuvette
number on the X axis and the Transmission [%] on the Y axis. The software turns
the rotor 200 rotor steps CCW and the CW with 2 steps displacement. For each
2 steps the transmission value is drawn. If the centred position is reached the
software turns the rotor 200 rotor steps CCW and the CW with 2 steps displace-
ment. For each 2 steps the transmission value is drawn. The resulting graph is
analyzed and the centre of the curve is used for the rotor home position. The
new parameter value is memorized.

Button TOOLS opens a dialogue with the button HARDCOPY to print out the
screen and QUIT to close the TOOLS screen.

Button OK leaves the screen.

 84

5.5.7  Button PIPETTOR

Functional principle
The Pipettor metal body is equipped with a self-adhesive heating foil including
an ntc temperature sensor. The heating foil is powered with a pwm voltage, as
part of a software control loop, which heats up the entire pipettor rotor. The
pipettor also is equipped with a capacitive level sensor to detect liquid surfaces
of very small liquid volumes to ensure minimal rest volumes. The pipettor is
moved by a DC servo motor in Z direction with a high positioning precision.

Figure 95

Section TEMPERATURE REGULATION

Figure 96

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Service Menu 85

Display field of the TARGET temperature [35…42°C]

Edit field for TARGET ADC digits [15…50°C]
Display field for ACTUAL measured digits
Display field for DIFFERENCE of digits (TARGET-ACTUAL)
Display field for PWM [0…77,5%] Remark: limited to 77,5 % because heating and
temperature measuring are performed in time multiplex within the period
Display of check box for STABLE. State STABEL is checked if ACTUAL digits are
TARGET digits ± 20 digits
Check box for REGULATION ON. If checked, the temperature regulation is acti-
vated.

Button CALIBRATE
If selected the numeric software keyboard is displayed and the user must enter
the TARGET temperature in °C, confirm with ENTER.
Next the numeric software keyboard is displayed again and the user must enter
the ACTUAL measured temperature in °C, confirm with ENTER. Remark: The Pi-
pettor temperature is measured by use of an external temperature measuring
device.
The software calculates the new TARGET digits value based on the TARGET and
ACTUAL temperature difference and an internal correlation function for the Pi-
pettor temperature. Target temperature is 37.5.

Section ACTUAL / TARGET X/Y/Z POSITIONS

Display field for the current X position in steps
Display field for the current Y position in steps
Display field for the current Z position in steps

Edit field for the target position in X and display of the limits for the X move-
ment [parameters].
Edit field for the target position in Y and display of the limits for the X movement
[parameters].
Edit field for the target position in Z and display of the limits for the X movement
[parameters].
If NO is selected, the movement is performed, if YES is selected, the Pipettor
remains in the same Z position.

Button STRESSTEST: If selected the XYZ Pipettor moves to stochastic XY Position.

If the Pipettor is not in Top Position (z=0), the Pipettor is moved to a safe z posi-
tion before the XY movements are started.
 86

Section LEVEL DETECTION

Display of the ACTUAL digits for the level detection converter
Display of the DRY THRESHOLD digits for the level detection converter
Display of the DIFFERNCE digits for the level detection converter (ACTUAL-DRY)
Display of the STATE for the level detection converter [DRY/WET]
Checkbox to activate the LEVEL DETECTION
Button TESTLOOPS
A dialogue is shown to decide if a level detection test at all top (z=0) position
have to be performed.

Figure 97

The dialogue must be quit with YES/NO. If YES is selected, the following test #1
is performed:
-- Display of a XY graph (X = position number, Y = level detection converter
value).
-- 3 times the Pipettor moves to all top positions.
-- For each of the 3 movements a line with different colors is drawn into the
XY graph.
-- At the end of the test the CV value is shown (must be below 0,05), Info mes-
sage must be quit with OK button.

Figure 98

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Service Menu 87

If NO is selected, a dialogue is shown with the option to start a level detection

test at the first Reagent position.

Figure 99

The same dialogue is displayed at the end of the test #1. If YES is selected the
following test #2 is performed:

-- Selection of one of the 5 defined vial types (user must place this vial with
liquid into target position).

Figure 100

-- Display of a XY graph (X = z position, Y = level detection converter value).

-- The pipettor searches for the level in the vial with 10 repetitions.
-- For each of the 10 movements a line with different colors is drawn into the
XY graph.
-- At the end of the test the CV value is shown (must be below 0,05), Info mes-
sage must be quit with OK button.
 88

Figure 101

If NO is selected, a dialogue is shown with the option to start a level detection

test at the first Sample position.

Figure 102

The same dialogue is displayed at the end of the test #1. If YES is selected the
following test #3 is performed:

-- Selection of one of the 4 defined vial types (user must place this vial with
liquid into target position).
.
Figure 103

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Service Menu 89

Figure 104

If NO is selected, the main screen of the PIPETTOR menu is displayed.

1 Button TOOLS opens a dialogue with the button HARDCOPY to print out the
screen and QUIT to close the TOOLS screen.
1 button OK leaves the screen.
 90

5.5.8  Dilutor
Functional principle
The Dilutor consists of a high resolution stepper motor with gear reduction for
the movement of the glass syringe and an electromagnetic 2-2 way valve for
switching between DI-pump flushing and syringe pipetting of the Pipettor.

Figure 105

Button DI-PUMP, selection will pump DI water for 10 seconds. A warning mes-
sage is displayed that the water may damage the analyzer. After confirmation,
the XYZ unit is powered off to enable moving of the arm to a save position man-
ually. After the test is done the user has to quit by an OK button and the XYZ
unit is powered on. The software monitors if the XYZ movements are within
allowed ranges. Otherwise a corresponding message is displayed and the user
must move the arm to a valid position.

Button WASTE PUMP, selection will power on the waste pump for 10 seconds. A
warning message is displayed that XYZ arm must be moved to an appropriate
position. After confirmation, the XYZ unit is powered off to enable moving of the
arm to a save position manually. After the test is done the user has to quit by
an OK button and the XYZ unit is powered on. The software monitors if the XYZ
movements are within allowed ranges. Otherwise a corresponding message is
displayed and the user must move the arm to a valid position.
Button CHANGE SYRINGE. If selected, the dosing unit is performing a home
command. Next the syringe is moved to position 4000. A message states that

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Service Menu 91

the user may now replace the syringe. After confirming the OK button the sy-
ringe is homed again.

Button PRECISION TEST: The syringe is moved to home position for 10 times. The
software monitors if the repetitions are within the specified tolerances of ± 1
step. In case this requirement is met the test is passed with OK. If the tolerances
are bigger, the test is failed and a corresponding message is displayed.

Figure 106

Edit field for the TARGET POSITION. The user can enter positions between 0 and
6556 steps. If the entry ranges are exceeded the software shows a warning mes-
sage and modifies the value to the min or max allowed value. Selecting OK will
drive the syringe to the desired position.

Display field for the ACTUAL POSITION always shows the current position of the
syringe.

Checkbox VALVE which powers on/off the electronic valve.

Read-only checkbox which is automatically checked if the syringe is positioned

in home (light barrier closed).

Button STRESS TEST which starts a syringe movement to random positions with-
in the allowed ranges. The test is stopped if the user again selects the STRESS
 92

TEST button. Before the test is started a warning message is displayed the sy-
ringe is subject to increased wear when performing this test. After the test is
finished, the syringe moves to the home position.

Display field MOVES: The quantity of strokes to stochastic target positions is

counted and displayed in the display field MOVES.

Button TOOLS opens a dialogue with the button HARDCOPY to print out the
screen and QUIT to close the TOOLS screen.

Button OK leaves the screen.

5.5.9  Shield
Functional principle
The shield of the analyzer is equipped with a magnetic sensor. The software de-
tects, if the shield is closed or open.

Figure 107

1 checkbox SHIELD CONTROL, if selected the software monitors the state of the
shield (closed / open). If checked the user must close the shield before a meas-
urement run can be performed. If not checked, the state of the shield sensor is
not used.

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Service Menu 93

1 read-only checkbox for the state of the COVER sensor, which is updated peri-
odically. If the shield is closed the software checks the box.

1 Button TOOLS opens a dialogue with the button HARDCOPY to print out the
screen and QUIT to close the TOOLS screen.

1 button OK leaves the screen.

5.5.10  Selftest
The separate SELFTEST functions are accessible by the button SETUP\SELFTEST
at password level ANYONE.
Only testing of module functions is possible, no modifications.
A log file of the SELFTEST actions is generated if an USB-Stick is attached to the
analyzer.

Figure 108
 94

5.5.10.1  REPORT area

Button SHOW displays the current Selftest log file.

ELECTRONICS area
Internal functions of the analyzer mainboard are checked. In case on any prob-
lems a corresponding message is displayed.
Information about the Selftest is stored in the log file, see section REPORT for
more details.

Button SYSTEM CLOCK tests the system clocks accuracy

Button HDP_TIMERS tests the function of the timers

Button SYSTEM-CALLS tests software function calls

Button I2C DEVICES tests the I2C communication to several external ICs

Button PUMPS tests the control circuit of the pumps including burn out pro-
tection circuit testing

Button CALCULATIONS tests the software functions for several calculations

Button EEPROM tests the EEPROM IC communication

5.5.10.2  MECHANICS area

Button PHOTOMETER & MIXER

Part 1: If selected the photometer is initialized and the LEDs for 740 nm are
powered on. A separate window is displayed containing the transmission values
for channel 1 and 2. The user is prompted to turn the measuring rotor centered
into the optics path to enable full light. If no cuvette ring is inserted, the values
for both channels should be 100% ± 2%.

Figure 109

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Service Menu 95

Part 2: The wavelength for 570 and 405 nm are tested

Part 3: The position of the measuring rotor encoder is checked for valid ranges:
0…13.000.
The Photometer test part 1…3 are finished with SUCCESS or FAILED which is en-
tered into the log file.
Part 4: A photometer mixer test is performed. Mixer 1 and mixer 2 are tested
alternating with increasing rpm values.

Figure 110

Start rpm = 100, Stop rpm = 1100, Increment = 200 rpm

The Mixer test part 4 is finished with SUCCESS or FAILED which is entered into
the log file.
At the end of the test on the analyzer LCD screen the result for the test is displa-
yed (PASSED / FAILURE) for 5 seconds.

Figure 111

Button STIRRER
If selected, the reagent stirrer motors for stirrer 1,2 and 3 are powered on sepa-
rately with increasing rpm target values.

Figure 112

Start rpm = 200

Increment rpm = 100
Stop rpm = 500
The software monitors the rpm value and reports if the test is PASSED or FAILED
into the report file and on the user LCD screen for 5 seconds.

Button PUMP TEST

If selected, the XYZ Servoboard is initialized and the Pipettor is moved to the
rinsing top position. Next the Selftest for the level detection is performed as
follows:
 96

-- State of external level sensors is checked

-- In case of failure a message is shown with the affected container sensor
-- Pump test phase 1 of 4: The dist. water pump and the waste water pump are
powered on to fill and to empty the rinsing station. The Pipettor checks the
liquid level position inside the rinsing station (a low liquid level is expected)
-- Pump test phase 2 of 4: The dist. water pump and the waste water pump are
powered on to fill and to empty the rinsing station. The Pipettor checks the
liquid level position inside the rinsing station (a low liquid level is expected)
-- Pump test phase 3 of 4: The dist. water pump is powered on to fill the rinsing
station. The Pipettor checks the liquid level position inside the rinsing station
(a high liquid level is expected)
-- Pump test phase 4 of 4: The dist. water pump and the waste water pump are
powered on to fill and to empty the rinsing station. The Pipettor checks the
liquid level position inside the rinsing station (a low liquid level is expected)
-- The software checks if the low and high values are within specific ranges,
depending on mechanical tolerances of the analyzer value and reports if the
test is ok or not into the selftest report file and on the user LCD screen.

In case of failure the message shown on screen contains the information which
phase of the pump test is affected. Depending from the message specific tasks
has to be performed (Check/cleaning of dist water system or waste water sys-
tem, Exchange of filter on dist. water sensor etc.) to solve the problem

Pump & Level Loop

After selecting the button “Pump&Level-Loop” a new screen is shown to select
the number of pump and level check cycles. Each cycle will empty the rinsing
station, will fill it again with distilled water for 2500ms and measures the liquid
level.

Figure 113

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Service Menu 97

After selecting the number of cycles a new screen appears to select the number
of repetitions for the level detection inside the rinsing station.

Figure 114

After selecting the number of repetitions the analyzer starts the “pump and lev-
el detection test loop”.

The result of the “pump and level detection test loop” is shown within a graphic
and an INFO message is displayed containing the information about the mini-
mal and maximum level raise inside the rinsing station. The message has to be
confirmed with “OK” to return to the selftest menu.

Figure 115

The difference between the two lines must be bigger than 115 steps
 98

Button VOLUME TEST

This menu allows the checking of one of the 6 existing volume curves:
-- 15mlHumGL
-- 12mlHumPL
-- 5mlHumGL
-- 5mlHumPL
-- CUP1ml
-- Washer
Remark: Names of the vials may be different since the names are parameters
which may be modified due to customer requirements.

Figure 116

Once a vial is selected, the following selections are available:

-- Vial type (reagent or washer)
-- Reagent-position [0…14] Take care that here Reagentposition 1 (labeling
rack) would be 0 ( in software), 5 would be 4 ….
After selection the XYZ unit is initialized and the following information are dis-
played (read only).

-- Name of volume curve vial

-- Base level [steps] (taken from TEACH-IN or parameter value)
-- Max-volume [1…1000000µl]
-- Rest volume [1…1000000µl]
-- Z-DIST = Distance added from Z-Quick to MAX Z
-- Dip-In [steps/µl]

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Service Menu 99

The software calculates the minimum quantity of pipetting steps based on the
entered information and the maximum volume / pipetting step of 250 µl in-
cluding all air gaps.

Figure 117

Button MEASURE: By a button MEASURE the software displays a message that

the user must place vial X with the volume Y into position Z. Selecting OK will
start the automatic procedure for the Volume Curve Creation:

-- Pipettor is rinsed for 10 seconds in the rinsing station

-- Loop start until rest volume is reached or max Z is reached (including DIP-IN)
• The Pipettor searches for the liquid level in the target vial.
• The software memorizes this Z step value to the corresponding volume
value.
• The calculated pipetting step volume is aspirated from the target vial.
• The Pipettor moves to the rinsing station and dispenses the aspirated
volume.
• Loop end

During the procedure the Volume/ Z-steps graphic is displayed in comparison to

the parameter values, stored in the analyzer flash memory.
 100

Figure 118

Button SHOW CURVES displays the current volume curve and the stored vol-
ume curve in one XY graph.

Figure 119

Button SHOW TABLE displays the parameters of the stored volume curve. The
data can be printed by a button PRINT.
Button OK will close the screen and memorize the changes.

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Service Menu 101

Button VOLUME FLOW

If selected, a message window is displayed with the instruction to place 3 empty
4 ml cups into the positions Control 1,2 and 3. The user can select YES to contin-
ue or NO to break.
If YES is selected, the following procedure is done
-- Initializing of XYZ Servo board.
-- Pipettor moves to position Control 1 Max z – 50 steps.
-- System pump pumps for 300 msec distilled water into the 4ml cup, placed
in position Control 1.
-- Pipettor detects level of liquid.
-- System pump pumps for 1000 msec distilled water into the 4ml cup, placed
in position Control 1.
-- Pipettor detects level of liquid.
-- Procedure is repeated for positions Control 2 and 3.
At the end of the procedure, the z position values for Control 1, 2 and 3 and level
1, level 2 and the difference are displayed. The difference between level 2 and
level 1 must be bigger than 300 z steps. The screen can be closed with the OK
button.

Button MOTORS TEST

If selected, a message is displayed, that the XY position test will be started and
the system would stop in case on errors.
The XYZ Servoboard is initialized and all positions are tested in the quick z po-
sition.

5.5.11  Webserver
Additionally a web server is implemented. In a browser window the user can
enter the IP address of the analyzer to monitor all IOs and to get information
about the following components:

Figure 120
 102

OPERATING SYSTEM
Display of Software details concerning the Multitasking system of the analyzer.

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Service Menu 103

INPUT / OUTPUT STATE

Display of information about the several IOs of the mainboard.

SOFTWARE UPDATE
Display of information about the last software update.
 104

ROTOR / SERVO:
Display of information about the state of the four DC servo motors.

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Service Menu 105

LIQUIDS
Display of information about loaded liquids.

QC STATE:
Display of information about the control periods.

SYS LOG:
Display of information about the currently performed actions.

PREVIOUS SYS LOG:

Display of information about the previously performed actions.

MEASURIONG RESULTS:
Display of information about the currently available measurement results of
the last or currently performed run.

SHOW / SAVE EXPORTED PARAMETERS:

Display of all parameters which are automatically exported to the SD card of the
mainboard.
 106

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Maintenance 107

6  MAINTENANCE

6.1  Maintenance frequency

Step Cycle Who

Frequency daily weekly monthly yearly User Service
Clean working plate X X
Run a flush cycle X X
Run a washing cycle using Washing X X
Solution
Clean Waster Water Tubing’s using X X
Cleaning Solution
Clean Pipettor tip X X
Clean Container DI Water and Sensor DI 2x X
Water (70% alcohol solution)
Replace distilled water in Container DI X X
Water
Clean Container Waste Water and Sen- X X
sor Waste Water (70% alcohol solution)
Fill Rinsing station with Cleaning, wait X X
2 minutes and run a flush cycle
Clean the housing X X
(70% alcohol solution)
Replace Filter for X X
Sensor DI Water
Check of sample positions insert in X X
working plate including single insert
segments (replace if needed)
Replace tubing’s X X
Pipettor <-> Valve
Valve <-> Di Water pump
Di water pump <-> Di water connector
Waste connector <-> waste pump
Waste Pump <-> Rinsing station
Waste sensor <-> Waste connector
Di Sensor <-> Di connector
Replace filter Di-Water X X
Check functionality of shield X X
According to Service Software
see chapter 5.5.9
 108

Step Cycle Who

Frequency daily weekly monthly yearly User Service
Replace Syringe and o ring, re-new in X X
Maintenance menu -> Syringe Mainte-
nance the counter
Check valve Dilutor for leakage or dirt X X
(replace if needed)
Check and clean Pipettor X X
(replace if needed)
Clean Measuring Rotor surface X X
(with alcohol)
Check Pipettor for Temperature X X
Perform Temp calibration according to
chapter Service Software 5.5.7
Reverse flush of Pipettor with hot dis- X X
tilled water Use Pipettor syringe from
the service tool kit
Recalibration of photometer (automat- X X
ed by service software) Perform accord-
ing to chapter Service Software 5.4.2
Optical inspection, cleaning and lubri- X X
cation of X/Y/Z cogwheel and toothed
rack
Clean and lubricate XYZ guide rails X X
Optical inspection of Measuring rotor X X
toothed belt and cogwheel
Optical inspection of Measuring rotor
X X
V-ball bearings for abrasion
Check of Measuring rotor temperatures. X X
Perform according to chapter Service
Software 5.5.3
Check of USB-port connectors for print- X X
er and USB-stick for wear out / function
Optical check of X/Y/Z flex cables for X X
sharp bendings
Clean Fan Power Supply each 2 X
years
Check motors and pumps for noise X X
Check all Pipettor Adjustments. Perform X X
according to Service Software 5.5.1

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Maintenance 109

Figure 121

Step Cycle Who

Frequency daily weekly monthly yearly User Service
Check Reagent Stirrer motors. Setup/ X X
Selftest/Reagent Stirrer The rotation
speed will be automatically checked by
the software.
Check the Dilutor. X X
Service/Setup/Dilutor /Precession test.
Test will be performed automatically.
Check Measuring Rotor.
X X
Service/Setup/Rotor/Stresstest and
regulation on.
Stabel should be ticked maximum after
15 min.
You have to stop the stresstest via
clicking again stresstest. The deviation
between target and actual pos should
be maximum 2.
Check Mixer-rotation in cuvette ring X X
Setup/Selftest/Photometer.
First remove cuvett ring for max trans-
mission.
After transmission test put cuvett ring
again in and check that mixer in pos
1&2 are rotating.
 110

6.2  Equipment

Figure 122 1. Use for X - Toothed rack and rack drive

Vaseline Grease 65510 2. Use for Y - Toothed rack and rack drive
3. Use for Z - Toothed rack and rack drive

Figure 123 1. Use for X - Guide rail

Ballistol Pointoiler 65509 2. Use for Y - Guide rail
3. Use for Z - Guide rail

6.3  Maintenance

Maintenance X - Toothed rack and rack drive

Figure 124 1. Maintenance X - Toothed

rack

Use only few technical

Vaseline.

Do not use grease.

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Maintenance 111

Maintenance Y - Toothed rack and rack drive

1. Maintenance Y - Figure 125

Toothed

Use only few technical

Vaseline.

Do not use grease.

Maintenance Z - Toothed rack and rack drive

1. Maintenance Z - Figure 126

Toothed

Use only few technical

Vaseline

Do not use grease

Maintenance X - Guide rail

1. Maintenance X - Guide Figure 127

rail
 112

Maintenance Y - Guide rail

Figure 128 1. Maintenance Y - Guide rail

Maintenance Y - Guide rail

Figure 129 1. Maintenance Z - Guide rail

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Troubleshooting 113

7  TROUBLESHOOTING

7.1  Error messages System

7.1.1  Movement YXZ

Error Cause Action

7.1.1.1
Z failure 1: 1. Blockage close to home position 1. Remove blocking parts
Blocked near target pos 2. Blocking particles on Z-rail 2. Remove blocking parts
3. Rail Z moves stiff 3. Clean and grease Rail Z
7.1.1.2 Z failure 2: blocked on way up 1. Blockage on way up 1. Remove blocking parts
2. Blocking particles on Z-rail 2. Remove blocking parts
3. Rail Z moves stiff 3. Clean and grease Rail Z
7.1.1.3 Z failure 3: blocked on way to Z 1. Blockage on way up 1. Remove blocking parts
2. Blocking particles on Z-rail 2. Remove blocking parts
3. Rail Z moves stiff 3. Clean and grease Rail Z
7.1.1.4 Z Home not done Motor Z does not reach home
position after initialization
1. Check cables and
1. Cables not properly connected connect properly
2. Cable failure 2. Replace cable
3. Motor failure 3. Replace motor
4. Electronic failure 4. Replace PCB
7.1.1.5 Pipettor blocked while moving Movement Z blocked while moving to 1. Remove blocking parts
upwards. Z target position = 0 z=0 2. Remove blocking parts
could not be reached
1. Pipettor movement blocked
2. Blocking particles on Z-rail
7.1.1.6 Pipettor blocked while moving Pipettor hits object while moving
downwards downwards (example load reagent)
1. Reagent vial is still covered 1. Remove lids from rea-
2. Washer vial is still covered gent vial
3. Blocking particles on Z-rail 2. Remove lid from wash-
4. X-home was not correct before. er vial
System has an offset in X for all 3. Remove blocking parts
positions. In this case the pipettor 4. Check home position
already hits on the working plate in- and correct movement
stead of the rinsing station opening
 114

Error Cause Action

7.1.1.7 Pipettor blocked while moving Target Z position =0 could not be 1. Remove blocking parts
upwards. Z target position ( = 0) reached exactly. 2. Remove blocking parts
could not be reached 1. Pipettor movement blocked
2. Blocking particles on Z-rail
7.1.1.8 Pipettor blocked while moving Movement Z when searching for 1. Only use specified
downwards. Z-quick position home position is blocked. reagent vials
could not be reached 2. Place reagent vial
1. Reagent vial too high properly
2. Reagent vial not placed properly 3. Check all x positions
3. Level detection, e.g. in wash station and correct alignment
at wrong x position is applicable
4. Pipettor movement blocked 4. Place missing vial
7.1.1.9 Pipettor movement blocked No liquid is detected at level detection
while liquid level detection process, e.g. load reagents.
1. Replace sample cup or
1. Pipettor hits ground of tube fill correctly.
2. Empty 5ml vial placed though a 2. Check and correct
15ml vial has been parameterized parameterization.
7.1.1.10 Pipettor movement blocked No liquid is detected at level detec-
while liquid level detection tion process, e.g. load samples.

1. Pipettor hits ground of tube. 1. Replace sample cup or

2. Empty short Cup (Hitachi) placed fill correctly.
though a Cup 4ml has been param- 2. Check and correct pa-
eterized. rameterization.
7.1.1.11 Y Home not done. Motor Y does not reach home position
after initialization.
1. Check cables and con-
1. Cables not properly connected nect properly.
2. Cable failure 2. Replace cable
3. Motor failure 3. Replace motor
4. Electronic failure 4. Replace PCB
7.1.1.12 X Home not done. Motor X does not reach home
position after initialization.
1. Check cables and con-
1. Cables not properly connected nect properly
2. Cable failure 2. Replace cable
3. Motor failure 3. Replace motor
4. Electronic failure 4. Replace PCB

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Troubleshooting 115

Error Cause Action

7.1.1.13 X movement blocked: Analyzer X home position won’t be reached
could not reach target position after selecting load reagent. 1. Only use specified
in X direction sample tubes
1. Sample tube too high. 2. Place sample tubes
2. Sample tube not placed properly . properly

7.1.2  Measuring rotor

Error Cause Action

7.1.2.1 Measuring rotor home not Measuring rotor does not reach home
reached position after initialization
1. Check cables and
connect properly
1. Cables not properly connected 2. Replace cable
2. Cable failure 3. Replace motor
3. Motor failure 4. Replace PCB
4. Electronic failure 5. Check and adjust
5. Toothed belt too strong adjusted Toothed belt
(tension). Servo motor does not 6. Check and adjust
reach home. Toothed belt
6. Toothed belt too weak adjusted
(tension). Servo motor moves on
when rotor is in home.
7.1.2.2. No cuvette ring or used cuvette 1. Cuvette ring is not inserted in cor- 1. Insert cuvette ring in
ring detected by Software rect position. correct position
2. Blocking particles, e.g. Mixer is un- 2. Remove blocking parti-
derneath the cuvette ring. cles
3. Measuring rotor home not correct 3. Check home
4. Cuvette ring is used (optical check 4. Use a new cuvette ring
of cuvette ring failed).
 116

7.1.3  Photometer

Error Cause Action

7.1.3.1 Lamp is weak
1. LED’s have lost energy. The mini- 1. Calibrate photometer
mum energy level of 50% from the in service software.
factory value is undershot. 2. If energy is too low
replace photometer.
7.1.3.2 F flag in measuring result 1. Internal Error in photometer. 1. Replace photometer
7.1.3.3 M flag in measuring result 1. Internal error in photometer mixer 1. Check cables to pho-
unit. tometer mixer.
2. Replace mixer pcb in
photometer unit.

7.1.4  Dilutor

Error Cause Action

7.1.4.1 Dilutor home not reached Dilutor does not reach home position
after initialization.
1. Check cables and
1. Cables not properly connected. connect properly
2. Cable failure 2. Replace cable
3. Motor failure 3. Replace motor
4. Electronic failure 4. Replace PCB
7.1.4.2 F flag in measuring result 1. Internal Error in photometer 1. Replace photometer

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Troubleshooting 117

7.1.5  Wash station / Tubing system

Error Cause Action

7.1.5.1 Wash station won’t be emptied Dilutor does not reach home position
after initialization

1. Pump waste water defect. 1. Replace Pump waste

2. Level detection defect, e.g. no water.
liquid in wash station. 2. Replace pcb pumps.
7.1.5.2 No water pumped into wash Wash station gets no water
station 1. Replace DI water pump
1. Pump DI water failure. 2. Check Pipettor tip.
2. Pipettor tip opening is blocked by 3. Check tubing system
particles. 4. Check external DI wa-
3. Leakage in tubing system. ter sensor (green).
4. DI water level sensor defect. 5. Check cable and Con-
5. Electronic failure. nectors of the Sensors.
7.1.5.3 Container waste water over- 1. Sensor waste water does not work 1. Replace sensor waste
flow 2. Wrong parameter in service soft- water
ware 2. Check parameter in
service software.
7.1.5.4 Wash station overflow 1. Pump waste water failure 1. Check waste pump
2. Leakage in tubing system 2. Check waste tubing’s.
7.1.5.5 Bad cleaning of Pipettor Pump DI Water carries not enough
liquid

1. Filter at Sensor DI Water plugged. 1. Replace filter

2. Valve failure (Dilutor) 2. Replace valve / pcb
3. Pipettor plugged 3. Clean Pipettor
4. Tubing plugged 4. Replace complete tub-
5. DI water pump volume flow too ing.
low. 5. Replace DI water pump
7.1.5.6 Bad results DI water system is dirty, e.g. alga 1. Replace Sensor DI Wa-
ter. Only use DI water
1. No DI Water for System liquid used. as system liquid
2. DI water system is dirty (alga’s). 2. Replace complete tub-
3. Pipettor is dirty. ing (alga’s).
3. Clean Pipettor with al-
cohol.
 118

Error Cause Action

7.1.5.7 Empty DI water detected by System water too warm (>+30°C)
software
1. Container DI Water is placed, e.g. in 1. Place container in
direct sunlight which causes high correct position, away
water temperature (Level detec- from direct sunlight
tion is managed via temperature and heat (radiator)
chances)

7.1.6  Temperature

Error Cause Action

7.1.6.1 Temperature at Pipettor too
1. Plug not correctly connected 1. Check connections
low
2. Cable defect 2. Replace cable
3. Pipettor failure 3. Replace Pipettor
4. Electronic failure in pcb that 4. Check and Replace pcb
controls Pipettor temperature board’s or cables
7.1.6.2 Temperature at measuring 1. Plug not correctly connected 1. Check connections
rotor too low 2. Cable defect 2. Replace cable
3. Pipettor failure 3. Replace Pipettor
4. Electronic failure 4. Check and Replace pcb
board’s or cables
7.1.6.3 Temperature at Pipettor too 1. Temperature setting too high 1. Calibrate pipettor
high 2. Electronic failure temperature in service
software
2. Replace pcb LSH-Pi-
pettor
3. Replace Pipettor incl.
flex cable
7.1.6.3 Temperature at measuring 1. Temperature setting too high/low 1. Calibrate measuring
rotor too high 2. Electronic failure rotor temperature in
service software
2. Check and Replace pcb
board’s or cables
7.1.6.3 Temperature at measuring 1. Temperature setting too high/low 1. Calibrate measuring
rotor too high 2. Electronic failure rotor temperature in
service software
2. Check and Replace pcb
board’s or cables

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Troubleshooting 119

7.1.7  Miscellaneous

Error Cause Action

7.1.7.1 Reagent stirrer position 1. No Teflon stirrer in reagent 1. Place magnetic Teflon stirrer to
without function vial reagent vial
2. Reagent stirrer motor 2. Replace pcb Reagent stirrer
failure motor
3. Electronic failure 3. Replace pcb Reagent stirrer
motor
7.1.7.2 Cuvette ring stirrer without 1. No mixer in cuvettes 1. Place new cuvette ring, incl. Mixer
function 2. Mixer motor failure 2. Replace mixer motor pcb
3. Electronic failure 3. Replace mixer motor pcb

For further error messages refer to User Manual.

7.2  General

Error Cause Action

7.2.1 No function 1. Instrument is off 1. Switch on analyzer

2. Power cable not connected 2. Attach power cable
correctly 3. Check primary voltage fuses in
3. Fuses not OK power filter.
4. Check fuses on pcb Mainboard
5. Check if all cables are on correct
plug positions
7.2.2 Fuse in power filter defect 1. Analyzer is used outside 1. Only use Instrument in permitted
the permitted voltage (100 - tension range.
–40V) range. 2. Use correct fuses
2. Wrong fuses used. 3. place power supply
3. Power wiring defect. 4. Replace power supply
4. Power supply failure

For further error messages refer to User Manual.

 120

7.3  Liquid system

7.3.1  Pipettor water jet

7.3.1.1  Check Pipettor water jet

1. 2. 3. 1. Water jet is OK
2. Water jet is fringed
3. Water jet is sloping

Flash the Pipettor

manually with tool set
and hot distilled water

7.3.2  Pipettor rinse (In case of stocked Pipettor tip)

7.3.2.1  Preparation rinse water system 1

Figure 130 1. Remove the reagent insert

2. Move the Pipettor manually to
the reagent hole (pull the Pipet-
tor to the top position while
moving it manually).

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Troubleshooting 121

7.3.2.2  Preparation rinse water system 2

1. Remove the reagent insert. Figure 131

2. Move the Pipettor manually to
the reagent hole (pull the Pipet-
tor to the top position while
moving it manually).

7.3.2.3  Remove tubing from dilutor

1. Remove the tubing from the Di- Figure 132

lutor valve.

Immediately eliminate leaking

liquids.

7.3.2.4  Remove tubing from dilutor

1. Push hot distilled water rear- Figure 133

wards through the Pipettor to
remove any parts from the in-
side.
2. Repeat this 2 times.

Use only hot ( ~ 50°C ) distilled

water.

Immediately eliminate leaking

liquids.
 122

7.3.3  DI Water System rinse (in case no DI Water flow)

7.3.3.1  Preparation rinse water system

Figure 134 1. Remove the reagent insert.

2. Move the Pipettor manually to
the reagent hole (pull the Pi-
pettor to the top position while
moving it manually).
3. Place a box under the Pipettor.

Pay attention to all applicable

safety rules

7.3.3.2  Rinse water system

Figure 135 1. Connect the DI Water Tool

(green clip) in the green connec-
tor.
2. Select RUN PREPARATION/
FLUSH 10 sec.
3. When the system in flushing
push hot distilled water into the
analyzer to fill the liquid system
with DI water.
4. Repeat this 2 times

Use only hot ( ~ 50°C ) distilled

water

Don’t use Waste Water Tool

(red clip) for DI Water System.

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Troubleshooting 123

7.3.3.3  Waste Water System rinse

1. Connect the Waste Water Tool Figure 136

(red clip) in the red connector.
2. Select RUN PREPARATION/
FLUSH 10 sec.
3. Draw up the syringe to generate
vacuum.
4. Repeat this 2 times

Don’t use Waste Water Tool

(red clip) for DI Water System Pay
attention to all applicable safety
rules.

Don’t use DI Water Tools

(green clip) for Waste Water
System.
 124

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Diagrams 125

8  DIAGRAMS

8.1  Analyzer Components

Figure 137

1 Pumps
2 Diluter
3 Pipettor
4 Measuring rotor
5 Display / Touch
6 Power supply and fan
7 Interface board
8 XYZ traverse
9 Washer / Reagent
positions
10 Sample / STAT
positions
11 Chip card reader
12 Photometer
 126

8.2  Wiring diagrams

8.2.1  Power Supply Unit

Figure 138

HumaClot Pro | Service Manual

Diagrams 127

8.2.2  Cardreader

Figure 139
 128

8.2.3  Dilutor / Pump Unit

Figure 140

HumaClot Pro | Service Manual

Diagrams 129

8.2.4  Measrotor and Photometer

Figure 141
 130

8.2.5  Reagent Stirrer, Cover Sensor and Barcode reader

Figure 142

HumaClot Pro | Service Manual

Diagrams 131

8.2.6  XYZ Unit

Figure 143
 132

8.2.7  PE

Figure 144

HumaClot Pro | Service Manual

Diagrams 133

8.2.8  Liquid System

Figure 145
 134

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Hardware and Adjustments 135

9  HARDWARE AND ADJUSTMENTS

9.1  Power filter suppresses

Figure 146
Task:

1. Power switch Analyzer, on / off 2

2. Main Fuse Analyzer, on / off
Fuse 250V, 5A, T 1
Don’t use other types of fuses.

9.2  PCB Boards

9.2.1  PCB Main board

9.2.1.1  PCB overview

Figure 147

Details see next pictures.

 136

9.2.1.2  Fuses and Lamps 1

Task:

1. Power XYZ Servo Motors, Valve,

on / off (green)
F4 Fuse 125V, 3A, T
2. Power Pipettor, on / off (green)
F1 Fuse 125V, 3A, T
3. Power Photometer, on / off
(green)
F11 Fuse 125V, 3A, T

Don’t use other types of fuses .

9.2.1.3  Fuses and Lamps 2

Task:

1. Power Dist Pump, Fan Power

supply, on / off (green)
F2 Fuse 125V, 3A, T
2. Power Waste Pump, Level senor,
Cover Locking,
on / off (green)
F10 Fuse 125V, 3A, T
3. Power MR Heating, on / off
(green)
F3 Fuse 125V, 3A, T

Don’t use other types of fuses .

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Hardware and Adjustments 137

9.2.1.4  Fuses and Lamps 3

Task:
Figure 148
1. Power Dilutor motor, on / off
(green)
F6 Fuse 125V, 3A, T
2. Power Cooling Block, on / off
(green)
F12 Fuse 125V, 3A, T
3. Power Filter wheel motor , on /
off (green)
F7 Fuse 125V, 3A, T

Don’t use other types of fuses

9.2.1.5  Fuses and Lamps 4

Task:
Figure 149
1. Power MR motor, Stirrer motor,
on / off (green)
F8 Fuse 125V, 3A, T
2. Power DC/DC Converter, on /
off (green)
F5 Fuse 125V, 3A, T
3. Power Barcode scanner, on / off
(green)
F9 Fuse 125V, 500mA, T

Don’t use other types of fuses.

 138

9.2.1.6  Lamps 4

Task:
Figure 150
1. Alive lamp, pulse (green)F6
Fuse 125V, 3A, T
2. LAN connected, on / off (red)

9.2.1.7  Reset

Task:
Figure 151
1. Push to reset the board

After reset the board is not

initialized

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Hardware and Adjustments 139

9.2.1.8  Battery

Task:
Figure 152
1. Battery, CR2032, 3V

9.2.1.9  CD Card

Task:
Figure 153
1. SD Card, micro, 8GB
 140

9.2.2  PCB XYZ Servo

9.2.2.1  Lamps

Task:
Figure 154
1. Alive lamp, pulse (green).

9.2.3  PCB Photometer

9.2.3.1  Lamps 1

Task:
Figure 155
1. Power +12V, on / off (green)
2. Power + 5V, on / off (green)
3. Power , on / off (green)
Fuse V, 2,5A, T

Don’t use other types of fuses.

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Hardware and Adjustments 141

9.2.3.2  Lamps and Fuse

Task:
Figure 156
1. Power lamp , on / off (green)
2. Function Alive lamp , on / off
(green)
3. Function RxD , on / off (green)
4. Function TxD , on / off (green)

9.3  PCB Housing lock

9.3.1  PCB Pumps

9.3.1.1  Lamp 5

Task:
Figure 157
1. Function Dist Pump, on / off
(green)
2. Function Waste Pump, on / off
(green)
Function Sensor rinsing, on / off
(green).
 142

9.3.2  PCB Valve

9.3.2.1  Lamp

Task:
Figure 158
1. Function Diluter Home (yellow)
2. Function Valve, on / off (green)

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Hardware and Adjustments 143

9.4  Mechanic

9.4.1  Measuring rotor

9.4.1.1  Tension roller bearing

1. Unfasten marked screws (2x) on the Figure 159

backside.
2. Hang up the weight on the pin of the
roller holder.
3. Stretch with 1000 g and turn multiple
the Cuvette rotor.
4. Fasten marked screws (2x).
5. Function Valve, on / off (green).

Alignment the weight vertical to the

module.
The position of the weight is essential
for the functionality.
 144

9.4.1.2  Toothed belt & Motor

Figure 160 1. Unfasten marked screws (4x)

2. Hang up the weight direct near the
screws of the motor holder.
3. Stretch with 500 g and turn multiple
the Cuvette rotor.
4. Fasten marked screws (4x)

Alignment the weight vertical to the

module
The position of the weight is essential
for the functionality.

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Hardware and Adjustments 145

9.4.2  Dilutor

9.4.2.1  Toothed belt & Motor

1. Unfasten marked screws (4x) Figure 161

2. Hang up the weight between
motor and cogwheel.
3. Stretch with 2000 g and turn
multiple the wheel.
4. Fasten marked screws (4x).

Alignment the weight vertical

to the module.
The position of the weight is
essential for the functionality.
 146

9.5  Interface extern

9.5.1  LAN extern

9.5.1.1  Position

Figure 162 1. RJ-45 female

PIN:
1. TX+
2. TX-
3. RX+
4. TRM+
5. TRM+
6. RX-
1
7. TRM-
8. TRM-

LED:
A. transfer
B. active

9.5.2  RS-232

9.5.2.1  Position

Figure 163 1. Mini DIN 6 female RJ-45 female

PIN:
1. WD DIS
2. GnD
3. CTS
4. n.c.
5. TXD
1 6. RTS
7. n.c.
8. RXD
9. n.c.

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Hardware and Adjustments 147

9.5.3  USB

9.5.3.1  Position USB 1

1. USB Standard, Type A Figure 164

PIN:
1. +5VDC
2. Data-
3. Data+
4. GND

9.5.3.2  Position USB 2

1. USB Standard, Type A Figure 165

PIN:
1. +5VDC
2. Data-
3. Data+
4. GND

1
 148

9.5.3.3  Position USB Device

Figure 166 1. USB Standard, Type B

PIN:
1. +5VDC
2. Data-
3. Data+
4. GND

9.5.4  Barcode scanner extern (Data)

9.5.4.1  Position
Figure 167 1. 9 x SUB-D female

PIN:
1. n.c
2. TxD
3. RxD
4. n.c
5. GND
6. n.c
7. n.c
1 8. n.c
9. 5VDC

This interface is disabled by

software.

Pin 9 is not supported in each

analyser. In this case use a sep-
arate Power supply.

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Hardware and Adjustments 149

9.5.4.2  Example for Adapter

Required setup for Scanner:
Baut rate: 9600 baud
Bit: 8 Bit
Stop bit: 1
Parity: no
Handshake: no

Use Operator Manual of Scanner

For 3rd party Scanner use external Power supply of Scanner.

Figure 168

9.5.5  Interface Sensor DI Water extern

9.5.5.1  Position

1. Mini connector 1 Figure 169

with bayonet lock

PIN:
1. Sen. 1
2. Shield
Sen. 2
 150

9.5.6  Interface Sensor DI WaSTE extern

9.5.6.1  Position

1. Mini connector with bayonet

Figure 170
1
lock

PIN:
1. Sen. 1
2. Shield
3. Sen. 2

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Replacement of Parts 151

10  REPLACEMENT OF PARTS

10.1  Dismantle Housing

10.1.1  Required tools

1. 1x Allen Wrench SW2,5

Figure 171

10.1.2  Switch off analyzer

1. Switch off analyzer
2. Remove power cable prior to any service operation

Pay attention to all applicable safety rules

Figure 172

2
 152

10.1.3  Secure Pipettor

1. Move Pipettor towards the highest level (Z-direction)

Make sure not to damage the Pipettor needle

Figure 173

10.1.4  Remove all loose parts

1. Remove all cups
2. Remove cuvette ring
3. Remove all adapter and vials
4. Remove the reagent tray

All loose parts must be removed from the analyzer

Figure 174

3
2

4 1

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Replacement of Parts 153

10.1.5  Remove reagent area

1. Take apart marked cable plug (1x)

Figure 175

10.1.6  Unfasten reagent stirrer plugs

1. Take apart marked cable plug (1x)

Figure 176

1
 154

10.1.7  Remove cover measuring rotor

1. Unfasten and remove marked screws (4x)

Figure 177

10.1.8  Remove front cover

1. Shift the clips

Figure 178

HumaClot Pro | Service Manual

Replacement of Parts 155

10.1.9  Remove top cover part 1

1. Unfasten and remove marked screws (2x)

Figure 179

10.1.10  Remove pipettor tubing from top cover

1. Pull out pipettor tubing from top cover

Figure 180

1
 156

10.1.11  Remove top cover part 2

1. Remove marked screws (2x)
2. Lift up the cover to remove it

Figure 181

10.1.12  Completely opened instrument

Figure 182

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Replacement of Parts 157

Figure 183

To re-install cover work flow will be in inverse order.

10.2  Pipettor

10.2.1  Required tools

Figure 184
 158

10.2.2  Unfasten tubing

1. Remove tubing from Pipettor end

Immediately eliminate leaking liquids

Figure 185 1

10.2.3  Unfasten cover

Figure 186

1. Unfasten and remove marked screws (2x)

2. Unfasten and remove marked screw (1x)
3. Remove cover carefully

HumaClot Pro | Service Manual

Replacement of Parts 159

10.2.4  Unfasten cable Z-motor

1. 1x Unfasten plug

Figure 187

10.2.5  Unfasten flex cable

1. Unfasten the flex cable
2. Apply magnets (tool box) on both ends of the Z guide rail to avoid sliding of
the rail out of the slider.

Figure 188
1
 160

10.2.6  Unfasten Pipettor

1. Hold on Pipettor.
2. Press the guide rail carefully downwards.

Make sure the needle of the Pipettor is free of any parts and won’t be
damaged.
Do not move the guide rail out of the guidance.

Figure 189
1
2

10.2.7  Unfasten guide rail 1

1. Slightly remove the guide rail from the Pipettor

Make sure the needle of the Pipettor is free of any parts and won’t be
damaged.
Do not move the guide rail out of the guidance.

Figure 190

HumaClot Pro | Service Manual

Replacement of Parts 161

10.2.8  Unfasten guide rail 2

Slightly remove the guide rail from the pipettor.
Slightly move the guide rail upwards.

Make sure the needle of the Pipettor is free of any parts and won’t be
damaged:
Do not move the guide rail out of the guidance.

Figure 191
1

10.2.9  Turn pipettor

1. Slightly turn the pipettor.

Make sure the needle of the pipettor is free of any parts and won’t be
damaged.
Do not move the guide rail out of the guidance.

Figure 192

1
 162

10.2.10  Remove the pipettor

1. Remove the pipettor upwards

Make sure the needle of the Pipettor is free of any parts and won’t be
damaged

Do not move the guide rail out of the guidance

Figure 193 1

10.2.11  Example: Secure guide rail

1. 1x Introduce a cable fixer on top
2. 1x introduce a cable fixer at bottom (or use magnets from the service tool
Kit)

Do not move the guide rail out of the guidance

Figure 194
1

To re-install work flow will be in inverse order.

HumaClot Pro | Service Manual

Replacement of Parts 163

10.3  Dilutor and pump angle

10.3.1  Required told

1. 1x Allen Wrench SW 2,5
2. 1x Allen Wrench SW 3,0
3. 1x Allen Wrench SW 4,0
4. 1x Allen Wrench SW 5,0
5. 1x Side cutter

Figure 195

10.3.2  Unfasten mounting screws)

1. Remove mounting screws (4x)

Figure 196

1
 164

Figure 197

10.3.3  Unplugging cable

1. Unplug red cable from Dilutor PCB
2. Unplug yellow cable from Pump PCB

Figure 198

HumaClot Pro | Service Manual

Replacement of Parts 165

10.3.4  Remove cable holder and grounding cable

1. Remove cable holder
2. Unplug grounding cable

Figure 199

1
2

10.3.5  Disconnect waste tubing from waste pump

1. Remove clip and waste tubing

Figure 200

1
 166

10.3.6  Remove pipettor tubing

1. Remove the pipettor tubing from the valve.

Figure 201

10.3.7  Unplug DI and Waste water sensor cables

1. Remove the cables for DI and waste water sensors connectors.

Now you can completely remove the angle with Dilutor and Pump modules.

Figure 202

1
To re-install work flow will be in inverse order.

HumaClot Pro | Service Manual

Replacement of Parts 167

10.4  Dilutor

10.4.1  Required tools

1. 1 x Allen Wrench SW 1.5
2. 1x Allen Wrench SW 3.0
3. 1x Allen Wrench SW 5.0

Figure 203

10.4.2  Removing mounting screws of Dilutor

1. Remove fixing screws of the dilutor (3x).

1 Figure 204
 168

10.4.3  Removing fixing screw of Syringe holder

1. Remove fixing screws of the dilutor (3x).

Figure 205

10.4.4  Remove Tubing DI water pump and Syringe holder

1. Remove DI water tubing
2. Remove syringe holder

Figure 206

HumaClot Pro | Service Manual

Replacement of Parts 169

10.4.5  Unplug valve cable from Dilutor PCB

1. Remove the valve cable from the dilutor PCB.

Now you can remove the Dilutor module from the mounting angle.

Figure 207
10.4.6  cable from Dilutor PCB

10.4.7  Removed Dilutor module

1. Remove the valve cable from the dilutor PCB.

Now you can remove the Dilutor module from the mounting angle.

Figure 208

To re-install work flow will be in inverse order.

 170

10.5  DI and waste water pump

1. 1x Allen Wrench SW 2
2. 1x Allen Wrench SW 3

10.5.1  Required tools

Figure 209

10.5.2  Unplug DI water pump from pump PCB

1. Unplug cables from pump PCB
2. Disconnect the tubing from DI water pump 1x Allen Wrench SW 3.

Figure 210

2
1

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Replacement of Parts 171

10.5.3  Disconnect Di water outlet tubing

1. Unplug cables from pump PCB .
2. Disconnect the tubing from DI water pump 1x Allen Wrench SW 3.

Now you can remove the DI water pump.

Figure 211

10.5.4  Disconnect the tubing from the waste water pump

1. Disconnect the tubing from the waste water pump.

Figure 212

1
 172

10.5.5  Removing screws of waste water pump

1. Unfasten screws of waste water pump (2x).

Figure 213 Now you can remove the waste water pump.

To re-install work flow will be in inverse order.

10.6  Display and Touch screen

10.6.1  Required tools

1. 1x Torx Wrench T10

Figure 214

HumaClot Pro | Service Manual

Replacement of Parts 173

10.6.2  Removing cover

1. Remove marked screws (4x)

Figure 215

10.6.3  Removing display

1. Unfasten marked screws (4x)

Figure 216

1
 174

10.6.4  Disconnect cables from Display

1. Disconnect the marked cables.

! Carefully remove the foil cable!

Figure 217

10.6.5  Already removed Display

Figure 218

To re-install work flow will be in inverse order.

HumaClot Pro | Service Manual

Replacement of Parts 175

10.7  Card reader

10.7.1  Required tools

1. 1x Torx Wrench T8

First same procedure as described in 1.6 Display and Touchscreen.

Figure 219

10.7.2  Unfasten screws

1. Remove the marked screws (2x)
2. Unplug the marked cable

Now you can remove the card reader.

Figure 220
2

To re-install work flow will be in inverse order.

 176

10.8  Power Supply

10.8.1  Required tools

1. 1x Allen Wrench SW 2,5
2. 1x Torx Wrench T10

Figure 221

10.8.2  Unfasten cables

1. Remove all cables which are crossing the cover of the power supply.

Unplug them from the mainbaord and fix them over the X guide rail to have a
free access to the power supply.

Figure 222
1

HumaClot Pro | Service Manual

Replacement of Parts 177

10.8.3  Open power supply cover

1. Remove marked screws (13x).

(All except of temp sensor and grounding point.)

Figure 223

10.8.4  Remove power supply and dejam coil

1. Remove marked screws (4x)
2. Remove marked screws (4x)

Figure 224

1
 178

10.8.5  Already removed power supply and dejam coil

Dejam coil left side
Power supply right side

Figure 225

To re-install work flow will be in inverse order.

10.9  Connection panel sensors

Figure 226
10.9.1  Required tools

HumaClot Pro | Service Manual

Replacement of Parts 179

10.9.2  Disconnect Sensors Di and Waste

Disconnect the liquid sensors

Figure 227

10.9.3  Unfasten tubing’s

1. Remove screw nuts
2. Remove the Tubings

Figure 228

To re-install work flow will be in inverse order.

 180

10.10  Valve

10.10.1  Required Tools

1x Allan Wrench 2.5
1x Allan Wrench 1.5

Figure 229

10.10.2  Remove mechanical valve

1. Disconnect tubing
2. Remove syringe
3. Remove marked screws (2)
Figure 230

HumaClot Pro | Service Manual

Replacement of Parts 181

10.10.3  Remove electronic valve

1. Remove marked screws (2) and disconnect cable.

Figure 231

10.10.4  Already removed valve

To re-install work flow will be in inverse order.

Figure 232
 182

10.11  Dilutor Motor

10.11.1  Required tools

1. 1x Allen Wrench SW 3.0

Figure 233

10.11.2  unfasten Cable

1. Unfasten cable

Figure 234

HumaClot Pro | Service Manual

Replacement of Parts 183

10.11.3  Remove screws

1. Remove marked screws (3)

Figure 235

10.11.4  Already removed Dilutor Motor

Figure 236

To re-install work flow will be in inverse order.

 184

10.12  PCB XYZ Servo

10.12.1  Required tools

1. 1x Torx Wrench T6
Figure 237

10.12.2  Unplug cables

1. Unplug marked cables

Figure 238

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Replacement of Parts 185

10.12.3  Removing screws

1. Remove marked screws (4x)

Figure 239

To re-install work flow will be in inverse order.

10.13  Rinsing Station Removing screws

10.13.1  Required tools

1. 1x Allan Wrench SW 2.5

Figure 240
 186

10.13.2  Remove screws

1. Remove marked screws (2)

Figure 241
1

10.13.3  Remove waste tubing

1. Remove waste tubing (open clip)

Figure 242

To re-install work flow will be in inverse order.

HumaClot Pro | Service Manual

Replacement of Parts 187

10.14  Measuring Rotor

1. 1x Allan Wrench SW 3.0

10.14.1  Required tools

Figure 243

10.14.2  Remove screws

1. Remove marked screws (4X)

Figure 244

1
 188

10.14.3  Unplug marked cables

1. Unplug marked cables

Figure 245

10.14.4  Already removed measuring rotor

Figure 246

HumaClot Pro | Service Manual

Replacement of Parts 189

10.15  Photometer

10.15.1  Required tools

1. 1x Allen Wrench SW 2.5

First proceed like described in 10.14 !

Figure 247

10.15.2  Remove screws

1. Remove marked screws (3x)

Figure 248

1
 190

10.15.3  Unplug marked cables

1. Unplug marked cables

Figure 249

10.15.4  Already removed Photometer

To re-install work flow will be in inverse order.

Figure 250

HumaClot Pro | Service Manual

Replacement of Parts 191

10.16  Mixer Board

1. 1x Allen Wrench SW 1.5

10.16.1  Required tools

Figure 251

10.16.2  Remove Screws

1. Remove marked screws (2x)

Figure 252

1
 192

10.16.3  Already removed mixer board

Figure 253

To re-install work flow will be in inverse order.

10.17  Motor X

10.17.1  Required tools

1. 1x Allen Wrench SW 2
2. 1x Allen Wrench SW 3
3. 1x Torx Wrench T6
4. 1x Torx Wrench T9

Figure 254

HumaClot Pro | Service Manual

Replacement of Parts 193

10.17.2  Remove cables

1. Remove cable

!Be careful with the flexcable!

Figure 255

10.17.3  Remove screws at PCB

1. Remove marked screws (4x)

Figure 256

1
 194

10.17.4  Remove YZ-Traverse

1. Unfasten screws and remove (2x)

Secure YZ Unit from dropping!

Figure 257

10.17.5  Unfasten X-Holder

1. Unfasten and remove marked screws (4x)
2. Remove X-holder

Figure 258

1 1

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Replacement of Parts 195

10.17.6  Unfasten X-Motor alignment

1. Remove screw including washer and spring.

Figure 259

10.17.7  Set free pin

1. Slightly press pin to set free.
2. Remove pin

Figure 260

1
 196

10.17.8  Alignment at assembly

1. Alignment X-Motor: Measure 17,4mm
2. The Alignment is important for later proper function!

!The cog wheel must be fixed straight in line and tight. It may not slide!
Figure 261

To re-install cover work flow will be in inverse order.

10.18  Motor Y
1. 1x Allen Wrench SW 2
2. 1x Allen Wrench SW 3
3. 1x Torx Wrench T6
4. 1x Torx Wrench T9

! Please remove Pipettor first!

! Do not move the guide rail out of the guidance!
!Secure guide rail from dropping!

Figure 262 10.18.1  Required tools

HumaClot Pro | Service Manual

Replacement of Parts 197

10.18.2  Unfasten cables

1. Remove marked cable

! Do not move the guide rail out of the guidance!

! Secure guide rail from dropping!
! Be carefully with the flex cable!

Figure 263

10.18.3  Unfasten YZ motor holder

1. Remove marked screws (4x)
2. Remove marked screw (1x)
3. Remove marked screws (3x)

3 2
Figure 264

1
 198

10.18.4  Set free pin and remove

1. Slightly press pin to set free
2. Remove the pin

! Secure spring for motor alignment!

Figure 265

To re-install cover work flow will be in inverse order

10.19  Motor Z
1. 1x Allen Wrench SW 2
2. 1x Allen Wrench SW 3
3. 1x Torx Wrench T6
4. 1x Torx Wrench T9

! Please remove Pipettor first!

! Do not move the guide rail out of the guidance!
! Secure guide rail from dropping!

10.19.1  Required tools

Figure 266

HumaClot Pro | Service Manual

Replacement of Parts 199

10.19.2  Remove cables

1. Remove plug x-motor
2. Remove cable plug
3. Remove cable plug

Carefully remove the cable plugs

Be carefully with flex cable

Figure 267

10.19.3  Remove YZ motor holder

1. Unfasten screws and remove (4x)
2. Unfasten screws and remove (1x)
3. Unfasten screws and remove (3x)

3 2
Figure 268

1
 200

10.19.4  Set free pin and remove

1. Slightly press pin to set free

Remove the pin.

! Secure spring for motor alignment!

Figure 269

To re-install cover work flow will be in inverse order.

HumaClot Pro | Service Manual

LIS Interface 201

11  LIS INTERFACE

For detailed information please refer to the LIS manual.

 202

HumaClot Pro | Service Manual

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