Scribd
Upload
79 views1 page

Media BPW Merck

Buffered Peptone Water (BPW) is a non-selective enrichment culture medium used to resuscitate injured bacteria, particularly pathogenic Enterobacteriaceae, from food and other materials. It complies with ISO standards for detecting salmonella. BPW is rich in nutrients to promote high bacterial growth rates and contains phosphate buffers that maintain pH and prevent bacterial damage. It undergoes sterilization at 121°C for 15 minutes then yields a clear yellowish broth upon preparation.

Uploaded by

Kesmavet UPTDLKK
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
79 views1 page

Media BPW Merck

Buffered Peptone Water (BPW) is a non-selective enrichment culture medium used to resuscitate injured bacteria, particularly pathogenic Enterobacteriaceae, from food and other materials. It complies with ISO standards for detecting salmonella. BPW is rich in nutrients to promote high bacterial growth rates and contains phosphate buffers that maintain pH and prevent bacterial damage. It undergoes sterilization at 121°C for 15 minutes then yields a clear yellowish broth upon preparation.

Uploaded by

Kesmavet UPTDLKK
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

ISO

Buffered Peptone Water (BPW)


For the preliminary, non-selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae, from foodstuffs
and other materials.
Buffered Peptone Water (BPW)

This culture media complies with the recommendations of the Experimental Procedure and Evaluation
International Standard Organisation ISO (ISO 6579-2002). Inoculate the culture medium with the sample material.
Horizontal method for the detection of salmonella spp.
Incubation: 16 - 20 hours at 37°C aerobically.
Mode of Action Transfer material from the resulting culture to a selective
The broth is rich in nutrients and produces high resuscitation enrichment culture medium recommended by the appropriate
rates for subletally injured bacteria and intense growth. The standard.
phosphate buffer system prevents bacterial damage due to
changes in the pH of the medium. Literature
DIN Deutsches Institut für Normung e.V.: Mikrobiologische Milchunter-
Typical Composition (g/litre) suchung. Nachweis von Salmonellen. Referenzverfahren. – DIN 10181.
Peptone from casein 10.0; sodium chloride 5.0; disodium DIN Deutsches Institut für Normung e.V.: Untersuchung von Fleisch und
hydrogen phosphate dodecahydrate 9.0; potassium dihydrogen Fleischerzeugnissen. Nachweis von Salmonellen. Referenzverfahren.
-DIN10160.
phosphate 1.5.
International Standard Organisation: Detection of salmonellae
Preparation (Reference method). International Standard ISO 6579 (2002).
Suspend 25.5 g/l, if desired, dispense into suitable containers, International Standard Organisation: Milk and Milk Products
autoclave (15 min at 121 °C). -Detection of Salmonella spp. ISO 6785 / IDF 93 (2001)

pH: 7.0 ± 0.2 at 25 °C. Ordering Information


The prepared broth is clear and yellowish.
Product Merck Cat. No. Pack size
Buffered Peptone Water 1.07228.0500 500 g
(BPW)
Buffered Peptone Water 1.07228.5000 5 kg
(BPW)

Quality control
Test strains Growth
Salmonella typhimurium ATCC 14028 good / very good
Escherichia coli ATCC 25922 good / very good
Enterococcus faecalis ATCC 33186 good / very good
Pseudomonas aeruginosa ATCC 27853 good / very good
Salmonella enteritidis ATCC 13076 good / very good

218 Merck Microbiology Manual 12th Edition

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy