KERALA TECHNOLOGICAL

UNIVERSITY

(THRISSUR CLUSTER - 07)

SCHEME AND SYLLABI

of

M. TECH.

in

INDUSTRIAL BIOTECHNOLOGY
(As on 03/09/2015)

OFFERING DEPARTMENT

BIOTECHNOLOGY ENGINEERING
CLUSTER LEVEL GRADUATE PROGRAM COMMITTEE

1. Dr Devdas Menon, Professor, IIT Madras,Chennai Chairman

2 Principal, Government Engineering College Trichur, Thrissur Convener

Principal, AXIS College of Engineering & Technology, East

3 Member
Kodaly, Murikkingal, Thrissur

4 Principal, IES College of Engineering, Chittilappilly,Thrissur Member

5 Principal, MET'S School of Engineering, Mala, Thrissur Member

Principal, Royal College of Engineering & Technology,

6 Member
Akkikkavu, Thrissur

Principal, Vidya Academy of Science & Technology,

7 Member
Thalakkottukara, Thrissur

Principal, Thejus Engineering College, Vellarakkad,

8 Member
Erumappetty, Thrissur

Principal, Universal Engineering College, Vallivattom,

9 Member
Konathakunnu ,Thrissur

Principal, Sahrdaya College of Engineering & Technology,

10 Member
Kodakara, Thrissur

ii
 CERTIFICATE
This is to certify that
1. The scheme and syllabi are prepared in accordance with the regulation and
guidelines issued by the KTU from time to time and also as per the decisions made
in the CGPC meetings.
2. The suggestions/modifications suggested while presenting the scheme and syllabi
before CGPC on 25.6.2015 have been incorporated.
3. There is no discrepancy among the soft copy in MS word format, PDF and hard
copy of the syllabi submitted to the CGPC.
4. The document has been verified by all the constituent colleges.

Coordinator in charge of syllabus revision of the Programme

Dr Ambili Mechoor
Professor
Department of Biotechnology Engineering
Sahrdaya College of Engineering and Technology
Kodakara,Thrissur.

Principal of the lead college

Dr Sudha George Valavi

Principal
Sahrdaya College of Engineering and Technology
Kodakara,Thrissur

Principals of the colleges in which the programme is offered

No Name of the College Principal’s Name Signature

1 Sahrdaya College of Engineering Dr Sudha George Valavi

and Technology

Date: Chairman
Place:

iii
 VISION

 To be a centre of excellence in the field of

Biotechnology.
 To focus on teaching and research in diversified
fields of Biotechnology.

MISSION

 To mould competent Biotechnologists.

 To become professionally fit, to transform the

acquired knowledge gained in Life Sciences and
Engineering by apt skills.

 To cope up with the challenges in the field of

Biotechnology.

iv
PROGRAM EDUCATIONAL OBJECTIVES
(PEOs)

i. To groom out globally efficient and ethically responsible Engineers to face

challenges in a fast moving society

ii. Equip the candidates to work collaboratively, creatively, and communicate

effectively in applying discipline-specific knowledge in basic sciences, chemical
engineering and biotechnology.

iii. Trouble shoot and analyze issue in the biotech industry and research arena with a
commitment to uncompromised product performance and a desire to obtain the
best possible environmental impact.

iv. Commitment towards continuous innovation in improving the products and

processes.

v
 PROGRAM OUTCOMES (POs)

i. Expertise knowledge in the application various biotechnological and

biochemical concepts in an industrial perspective.

ii. Demonstrate and application of knowledge for innovative and modern

engineering applications.

iii. Acquire professional and effective communication skills.

iv. Potential to apply mathematical concepts to resolve critical and practical

oriented real time industrial challenges.

v. Pursue research to enhance the existing pool of knowledge.

vi. Evolve professional and ethical attitudes to accepted socially responsible

citizens.

vii. Understand and corroborate the global biotech industry business and
management.

viii. Demonstrate and perceive their role as engineers or entrepreneurs to the

overall development of the society.

ix. Design and analyze biological reactors for production of industrially important
products (bio molecules).

x. Develop Technological innovations for achieving cleaner production and

sustainableindustrial growth.

xi. Design of innovative processes and products whose performance cannot be

achievedusing conventional methods

xii. Address the demand for sustainable supply of fuels and food through biological
means.

vi
KERALA TECHNICAL UNIVERSITY

M.Tech DEGREE COURSE

IN
INDUSTRIAL BIOTECHNOLOGY

(DEPARTMENT OF BIOTECHNOLOGY ENGINEERING)

Proposed Curricula, Scheme of Examinations & Syllabi

(With effect from 2015 admissions)

(PROPOSED)

1
 SCHEME OF EXAMINATIONS

Semester 1

Exam Course Name L- T - P Internal End Semester Exam Credits

Slot No: Marks Marks Duration (hrs)
Applied
A 07MA6007 Mathematics For
Biotechnology 4-0-0 40 60 3 4
Molecular
Biology
B
07BT6203 &Immuno-
technology 4-0-0 40 60 3 4
Fermentation &
C 07BT6205 Enzyme
Engineering 4-0-0 40 60 3 4
Advanced
D 07BT6207
Bioinformatics 3-0-0 40 60 3 3
E 07BT6209X Elective I
3-0-0 40 60 3 3

07GN6001 Research 0-2-0 100 0 0 2

Methodology
Immunology &
07BT6213 Molecular 0-0-2 100 0 0 1
Biology Lab
07BT6215 Introduction to
0-0-1 - - - -
Seminar
Total Credits :21

2
Semester :2

End Semester Exam

Internal
Exam Course No: Name L- T - P Credits
Marks
Slot Marks Duration (hrs)
Animal and Plant
A
07BT6202 cell Culture 4-0-0 4 60 3 4
Analytical
B 07BT6204 Techniques in
Biotechnology 3-0-0 40 60 3 3
Transport
phenomena in
C
07BT6206 Bioprocess
system 3-0-0 40 60 3 3
D 07BT6208X Elective II 3-0-0 40 60 3 3
E 07BT6210X Elective III 3-0-0 40 60 3 3
07BT6212 Seminar 0-0-2 100 0 0 2
07BT6214 Mini Project 0-0-4 100 0 0 2
Bioprocess and
07IB6216 Fermentation
Technology Lab 0-0-2 100 0 0 1
Total Credits : 21

Semester : 3
End Semester Exam
Exam Internal
Course No: Name L- T - P Credits
Slot Marks
Marks Duration (hrs)
A 07BT7201X Elective IV 3-0-0 40 60 3 3
B 07BT7203X Elective V 3-0-0 40 60 3 3
07BT7205 Seminar 0-0-2 100 0 0 2
07BT7207 Project 0-0-12 50 0 0 6
(Phase 1)
Total Credits :14

Semester : 4
End Semester Exam
Exam Internal Credits
Course No: Name L- T - P
Slot Marks Duration
Marks
(hrs)
Project
07BT7202 (Phase 2) 0-0-21 100 0 0 12 12
Total credits: 12

L-Lecture,T –Tutorial, P-Practical; TOTAL CREDITS: 68

3
LIST OF ELECTIVE COURSES OFFERED

Semester I

ELECTIVE: I

07BT62091- Clinical Biotechnology

07BT62093- Biopharmaceutical& Pharmaceutical Technology
07BT62095- Biopolymer Technology
07BT62097-Protein Engineering

Semester II

ELECTIVE: II

07BT62082- Bioprocess Models: Design and Stability

07BT62084-Food Processing Technology
07BT62086-Metabolic Engineering
07BT62088-Bioprocess Modeling& Simulation

ELECTIVE: III

07BT62102- Molecular Diagnostics

07BT62104-Bioreactor Design
07BT62106-Genomics& Proteomics
07BT62108-Bio-fuel Engineering

Semester III
ELECTIVE: IV

07BT72011-Advanced Bio separation Techniques

07BT72013-Intellectual Property Rights (IPR) for a Global Bio-economy
07BT72015-Molecular Modeling & Drug Discovery
07BT72017-Fundamentals of Synthetic Biology

ELECTIVE: V

07BT72031-Tissue Engineering & Biomaterials

07BT72033-Nanobiotechnology
07BT72035-Management, Entrepreneurship & Bio-business
07BT72037-Structural Biology

4
FIRST SEMESTER

5
Course No: 07MA6007
Course Title:APPLIED MATHEMATICS FOR BIOTECHNOLOGY
Credits: 4-0-0:4
Year :2015
Pre-requisites: Nil
Course Objectives:
 To facilitate the student with necessary helping tools and to understand the design
aspects and the kinetic parameters.

Course Content/ Syllabus :

Finding a basic feasible solution, testing for optimality for transportation problems.Newton’s
forward and backward interpolation formula.Probability& Statistics,
Baye’stheorem.Sampling distributions. Testing of Null hypothesis and testing errors. Curve
fitting, Correlation and Regression. Design of experiments : Principles of experimentations.
Cluster analysis and trees, ANOVA.

Course Outcome:
 The student will be able to have basic knowledge in fields of transportation,
interpolation, probability, statistics, curve fitting and design of experiments.
 The student will also be able to apply in biological sciences.

Text Books:
1. Introduction to Numerical Analysis (Second Edition), By Hildebrand F.B., Dover
Publications, 1987.
2. Richard A Johnson, CB Gupta, Miller and Freund's,' Probability and statistics for
engineers', 7th edition, Pearson education.

References

1. Higher Engineering Mathematics 37th edition. By Grewal.

2. Numerical methods, By Kandasamy P., Thilagavathy K., Gunavathy K., S. Chand &
Co.,2003.
3. Optimization methods in Operations research and Systems analysis, By Mital K.V. and
Mohan C., New Age Publications, 1996.
4. Fundamentals of Operations research, By Ackoff and Sasieni, M.W., Wiley, 1968.
5. Operations Research, By Verma A.P., Kataria and sons, 2001.
6. Comprehensive Statistical methods By P. N arora, SumeetArora, S. Arora. S Chand & Co.
7. Advanced Engineering Mathematics, By E. Kreyszig 8th Edition.
8. Mathematical Statistics, By V C Kapoor& Gupta

6
 COURSE PLAN

COURSE NO: 07MA6007

COURSE TITLE: APPLIED MATHEMATICS FOR BIOTECHNOLOGY
(L-T-P : 4-0-0) CREDITS:4

MODULES Contact Sem.Exam

Hours Marks;%

9 15
MODULE: 1 Transportation: Introduction, transportation problems,
finding a basic feasible solution, testing for optimality, Assignment
problems.

9 15
MODULE: 2 Interpolation: Introduction, Newton’s forward
interpolation formula for equal intervals, Newton’s backward
interpolation formula, error in polynomial
FIRST INTERNAL TEST

MODULE: 3 Probability & Statistics: Probability –n Addition theorem, 10 15

Multiplication theorem and conditional probability – Baye’s theorem.

8 15
MODULE: 4Sampling distributions – Large samples and Small
samples. Testing of Null hypothesis- Z test, t Test, student t test, paired t
test, and χ2 test. Type I and Type II errors. Fishers F test. Goodness of
fit. Taguchi’s statistical methods.
SECOND INTERNAL TEST

10 20
MODULE: 5 Curve fitting: Curve fitting – fitting a straight line and
second degree curve. Correlation and Regression; R2. Fitting a non
linear curve. Bivariate correlation application to biological sciences.

10 20
MODULE:6Design of experiments: Principles of experimentations.
One way – Two way classifications- Randomized Block designs – Latin
Square Designs, Factorial experiment. Multivariate analysis, cluster
analysis and trees, ANOVA

END SEMESTER EXAMINATION

7
ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT6203

Course Title:MOLECULAR BIOLOGY AND IMMUNOTECHNOLOGY
Credits: 4-0-0:4
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Aims for preparing the students for a career in applied research in molecular biology
& immunology in therapeutic and technological innovations
Course Content/ Syllabus:
Organization of Nucleic Acids & Gene Expression.Replication in Prokaryotes and
Eukaryotes. Transcription, translation, posts translational Modifications. Recombinant DNA
Technology &Applications.Enzymes in Genetic Engineering.The hybridization
reaction.Construction of Genomic and cDNA libraries and screening.Biology of complement
systems.Purification of mononuclear cells from peripheral blood.Regulation of gene
expression.Vaccines.Immune diagnosis of Infectious diseases - immuno screening of
recombinant library.

Course Outcome:

1. To understand the fundamental principles behind nucleic acids organization and gene
expression.
2. To gain in depth knowledge in rDNA technology, immunotechnology and its
applications.
Text Books:
1. David Friefelder, Molecular Biology (2e), Jones and Bartlett Publishers Inc, 1987.

8
 2. Primrose S.B and R. W. Old, Principles of gene manipulation - An introduction to
genetic engineering (Vol. 2), Blackwell Scientific Publications, 1980.
3. Immunology, Richard A. Goldsby, Thomas J. Kindt. Barbara, A. Osborne, Janis Kuby
5th Edition, 2003. W. H. Freeman & Company.
4. Immunology, L.M. Roitt, J. Brestoff and D.K. Male, 1996.

References:
1. Walker J.M and R.Rapley, Molecular Biology and Biotechnology, Indian Reprint
byPanima Publishing Corporation, 2000.
2. Sambrook J et al, Molecular Cloning (Vol I, II and III), Cold Spring Harbor
Laboratory(CSHL) Press, 1989
3. Immuno-biology, Janeway CA and Paul Travers 1994.
4. Immunological Techniques, D.M. Weir, 1992

COURSE PLAN
COURSE NO: 07BT6203
COURSE TITLE: MOLECULAR BIOLOGY AND IMMUNOTECHNOLOGY
(L-T-P : 4-0-0) CREDITS:4

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Organization of Nucleic Acids & Gene

Expression:Structure and Different forms of DNA and RNA,
Organization of DNA in Prokaryotic and Eukaryotic Chromosomes. 8 15
DNA Replication in Prokaryotes and Eukaryotes, Telomeric
Replication in Eukaryotes. Transcription, translation, posts
translational Modifications.

MODULE: 2 Recombinant DNA Technology & Applications:

Introduction to cloning, Method of creating recombinant DNA
molecules, Cloning Vectors, Expression Vectors. Enzymes in Genetic 10 15
Engineering. The hybridization reaction, Production and Labeling of
Gene Probes, Southern Blotting, Northern Blotting, in situ
hybridization.

FIRST INTERNAL TEST

MODULE: 3 Construction of Genomic and cDNA libraries and

screening. Applications of Recombinant DNA Technology as
Diagnostic Tools- SNPs, VNTRs, Drugs and Therapies- Therapeutic 9 15
proteins from Transgenic plants and animals, Gene Therapy
Combating Disease. DNA fingerprinting, Directed mutagenesis,
Antisense Technology.

MODULE: 4 Biology of complement systems - structure and function

of MHC class I and II molecules - antigen recognition and presentation
- humoral and Cell mediated immune responses - hypersensitivity
reaction - immune suppression and immune tolerance - auto immune 9 15

9
disorders. antigen - antibody reaction in immunodiagnostic; hybridoma
and monoclonal antibody production; - human monoclonal antibodies;
catalytic antibodies - complement fixation.

SECOND INTERNAL TEST

MODULE: 5 Purification of mononuclear cells from peripheral blood

.fluorescent activated cell sorter - mitogen and antigen induced
lympho-proliferation assay - cell mediated lympholysis - mixed 10 20
lymphocyte reaction. - macrophage cultures and macrophage
activation .

MODULE : 6 Regulation of gene expression- The Operon Concept –

Promoter, Operator, Terminator, Attenuator, Inducer, Repressor, Effect
of cAMP Complex; lac operon.DNA Repair, Mutagenesis and
Mutations. generation of T cell clones - HLA typing. Biology and 8 20
assay of cytokines. Vaccines. Immune diagnosis of Infectious diseases
- immuno screening of recombinant library.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT6205

Course Title:FERMENTATION AND ENZYME ENGINEERING
Credits: 4-0-0:4
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Aims at transformation and industrialization of industrial enzyme,
 New technical innovation of fermentation engineering,
 High expression and mass preparation of proteins.
Course Content/ Syllabus:

Fermentation &processing.Medium requirements for fermentation processKinetics of

substrate utilization, product formation and biomass production.Simple unstructured kinetic

10
Models for microbial growthStructured kinetic Models. The production of some
commercially important Organic acids, amino acids and alcohols, study of production
processes for various classes of low molecular weight secondary metabolites. Principles of
enzyme catalysis.Industrial utilization of enzymes.

Course Outcome:
1. To familiarise the fermentation and processing techniques
2. To understand the kinetics of substrate utilization, product formation and biomass
production
Text Books:
1. Cruger.W and A.Cruger, A Textbook of Industrial Microbiology (2e), Sinauer
Associates, Sunderland,US, 2004.
2. Michael Shuler and FikretKargi, Bioprocess Engineering: Basic Concepts (2e),
Prentice Hall, Englewood Cliffs, NJ, 2002.

References:
1. Stryer.L, Biochemistry (4e), Freeman, 2002.
2. Bailey .J.E and D. F. Ollis, Biochemical Engineering Fundamentals (2e), Mc-Graw
Hill, Inc., 1986.
3. Pauline M Doran, Bioprocess engineering principles (1e), Academic Press, 1995.
4. Principles of Biochemistry, AL. Lehninger, D.L. Nelson and M. M. Cox. 1993. Worth
Publishers, New York

COURSE PLAN
COURSE NO: 07BT6205
COURSE TITLE: FERMENTATION AND ENZYME ENGINEERING
(L-T-P : 4-0-0) CREDITS:4

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Fermentation& processing: Introduction to

fermentation technology: Upstream and downstream processing of
biomolecules. Isolation, Preservation and Improvement of
Industrial Micro-Organisms; Medium requirements for 10 15
fermentation process; Criteria for good medium; Sterilization -
batch and continuous heat sterilization of liquid media, filter
sterilization of liquid media and Air. Design of sterilization
equipment.

MODULE: 2 Kinetics of substrate utilization, product formation

and biomass production: Phases of cell growth in batch cultures -

11
transient growth kinetics, Simple unstructured kinetic Models for 8 15
microbial growth, Growth of filamentous organisms;
Environmental conditions affecting growth kinetics, substrate and
product inhibition on cell growth and product formation

FIRST INTERNAL TEST

MODULE: 3 Structured kinetic Models, segregated kinetic

Models of growth. Production of primary and secondary
metabolites. The production of some commercially important
Organic acids, amino acids and alcohols, study of production 8 15
processes for various classes of low molecular weight secondary
metabolites: Antibiotics, quinones, aromatics, Vitamins and
Steroid.

MODULE : 4 Principles of enzyme catalysis: Proteins as

enzymes; Classification of Enzymes; Mechanism of Enzyme
Action; Determination of elementary step rate kinetics, patterns of 10 15
substrate concentration dependence, Modulation and regulation of
enzyme activity .

SECOND INTERNAL TEST

MODULE: 5Industrialapplication of enzymes:Immobilized

enzymes - principles & techniques of immobilization -
commercial production of enzymes; amylases, proteases, 9 20
cellulose, artificial enzymes, Enzyme Modification - site directed
mutagenesis; immobilized enzyme in industrial processes.

MODULE: 6 Structure and function of coenzyme - reactions

involving TPP, pyrodoxal phosphate, nicotinamide, flavin
nucleotide, coenzyme A and biotin. Industrial utilization of 9 20
enzymes, food, detergents, energy, waste treatment,
pharmaceuticals and medicine.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

12
Course No: 07BT6207
Course Title:ADVANCED BIOINFORMATICS
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Basic Knowledge in Bioinformatics

Course Objectives:
 Provide critical tools for managing the immense volume of biological data and
increase the efficiency of drug discovery and development.

Course Content/ Syllabus:

Scope of Bioinformatics, Applications in various fields. Databases, Hidden Markov Models.

Applications.Information retrieval from biological databases.Programming skills in
Bioinformatics and PERL.Comparative genomics. Phylogenetic analysis, Sequence
annotation: principles of genome annotation- annotation tools & resources. Virtual screening
and compound ranking/scoring.Receptor-ligand interactions analysis. Scaffold hopping, 3D
database screening. Explicit/implicit solvation Models

Course Outcome:
1.To familiarise with the algorithms working behind Bioinformatics software.

2. To improve the programming skills in Perl programming..

Text Books:

1. Bioinformatics, David.W.Mount
2. Essential Bioinformatics, Jin Xion

References:

1. Bioinformatics, Andreas D. Baxevanis

2. Perl for Bioinformatics, James Tisdall

13
 COURSE PLAN
COURSE NO: 07BT6207
COURSE TITLE: ADVANCED BIOINFORMATICS
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Scope of Bioinformatics, Applications in various fields, 5 15

Types of Databases, Hidden Markov Models. Applications: Modeling
Protein sequence families, multiple alignments.

MODULE: 2 Nucleotide databases, Protein databases, specialized 5 15

databases, Disease databases, information retrieval from biological
databases.

FIRST INTERNAL TEST

MODULE: 3 Programming skills in Bioinformatics, PERL: 10 15

Introduction and basics of PERL, variables, numbers, operators, loops,
Arrays, Hashes, Control structures, File handling, strings
manipulations, regular expressions.

MODULE: 4 Comparative genomics, Motif representation:

consensus, regular expressions; PSSMs; phylogenetic analysis-steps in 10 15
phylogenetic analysis, Sequence annotation: principles of genome
annotation- annotation tools & resources.

SECOND INTERNAL TEST

MODULE: 5 Virtual screening and compound ranking/scoring.

Receptor-ligand interactions analysis, Fragment-based design, de novo 6 20
design (LUDI), Pharmacophore generation (Catalyst)

MODULE: 6 Scaffold hopping, 3D database screening.

Explicit/implicit solvation Models, Protein Modeling (MODELER®) 6 20
and analysis. Protein-protein docking and refinement.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

14
 Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07GN6001`

Course Title:RESEARCH METHODOLOGY
Credits: 0-2-0:2
Year: 2015
Pre-requisites: Nil
Course Objectives:
The main objective of the course is to provide a familiarization with research methodology and to
induct the student into the overall research process and methodologies. This course addresses:

 The scientific research process and the various steps involved

 Formulation of research problem and research design
 Thesis preparation and presentation.
 Research proposals, publications and ethics
 Important research methods in engineering

As a tutorial type course, this course is expected to be more learner centric and active involvement
from the learners are expected which encourages self study and group discussions. The faculty mainly
performs a facilitator’s role.

Course Content/ Syllabus:

Overview of research methodology - Research process, scientific method, research design process.
Research Problem and Design - Formulation of research task, literature review, web as a source,
problem solving approaches, experimental research, and ex post facto research.
Thesis writing, reporting and presentation -Interpretation and report writing, principles of thesis
writing- format of reporting, oral presentation.
Research proposals, publications and ethics - Research proposals, research paper writing,
considerations in publishing, citation, plagiarism and intellectual property rights.
Research methods – Modelling and Simulation, mathematical modeling, graphs, heuristic
optimization, simulation modeling, measurement design, validity, reliability, scaling, sample design,
data collection methods and data analysis

Course Outcome:

15
At the end of course, the student will be able to:
 Discuss research methodology concepts, research problems, research designs, thesis
preparations, publications and research methods.
 Analyze and evaluate research works and to formulate a research problem to pursue research
 Prepare a thesis or a technical paper, and present or publish them
 Apply the various research methods followed in engineering research for formulation and
design of own research problems and to utilize them in their research project.
 Text Books:
1. David Friefelder, Molecular Biology (2e), Jones and Bartlett Publishers Inc,
1987.
2. Primrose S.B and R. W. Old, Principles of gene manipulation - An
introduction to genetic engineering (Vol. 2), Blackwell Scientific
Publications, 1980.
3. Immunology, Richard A. Goldsby, Thomas J. Kindt. Barbara, A. Osborne,
Janis Kuby 5th Edition, 2003. W. H. Freeman & Company.
4. Immunology, L.M. Roitt, J. Brestoff and D.K. Male, 1996.

References:
1. Walker J.M and R.Rapley, Molecular Biology and Biotechnology, Indian Reprint
byPanima Publishing Corporation, 2000.
2Sambrook J et al, Molecular Cloning (Vol I, II and III), Cold Spring Harbor
Laboratory(CSHL) Press, 1989
3.Immuno-biology, Janeway CA and Paul Travers 1994.
4Immunological techniques, D.M. Weir, 1992

COURSE PLAN
COURSE NO: 07GN6001
COURSE TITLE: RESEARCH METHODOLOGY
(L-T-P : 0-2-0) CREDITS:2

MODULES Contact Sem.Exam

Hours Marks;%

MODULE 1- Overview of Research Methodology

Research concepts – meaning – objectives – motivation - types of
research –research process – criteria for good research – problems
encountered by Indian researchers - scientific method - research 5 10
design process – decisional research

16
MODULE 2- Research Problem and Design
Formulation of research task – literature review – methods –
primary and secondary sources – web as a source –browsing tools
-formulation of research problems – exploration - hypothesis
5 10
generation - problem solving approaches-introduction to
TRIZ(TIPS)-experimental research – principles -Laboratory
experiment - experimental designs - ex post facto research -
qualitative research

FIRST INTERNAL TEST

MODULE 3- Thesis writing, reporting and presentation 4 15

Interpretation and report writing – techniques of interpretation –
precautions in interpretation – significance of report writing –
principles of thesis writing- format of reporting - different steps in
report writing – layout and mechanics of research report -
references – tables – figures – conclusions. oral presentation –
preparation - making presentation – use of visual aids - effective
communication

MODULE 4 -Research proposals, publications, ethics and IPR 5 15

Research proposals - development and evaluation – research paper
writing – layout of a research paper - journals in engineering –
considerations in publishing –scientometry-impact factor- other
indexing like h-index – citations - open access publication -ethical
issues - plagiarism –software for plagiarism checking- intellectual
property right- patenting case studies

SECOND INTERNAL TEST

MODULE 5- Research methods – Modelling and Simulation

Modelling and Simulation – concepts of modelling – mathematical
modelling - composite modelling – modelling with – ordinary 5 10
differential equations – partial differential equations – graphs
heuristics and heuristic optimization - simulation modelling

MODULE 6 – Research Methods – Measurement, sampling

and Data acquisition
Measurement design – errors -validity and reliability in
measurement - scaling and scale construction - sample design - 4 10
sample size determination - sampling errors - data collection
procedures - sources of data - data collection methods - data
preparation and data analysis

THIRD INTERNAL TEST

Internal continuous assessment: 100 marks

17
Internal continuous assessment is in the form of periodical tests and assignments. There arethree tests
for the course (3 x 20 = 60 marks) and assignments (40 marks). The assignments can be in the form of
seminar, group tasks, case studies, research work or in a suitable format as decided by the teacher.
The assessment details are to be announced to students at the beginning of the semester by the
teacher.

Course No: 07BT6213

Course Title:IMMUNOLOGY AND MOLECULAR BIOLOGY LAB

Credits: 0-0-2:2

Year :2015

Pre-requisites: Nil
Objectives:
 To provide hands on training towards the application of molecular techniques and
products of industrial importance
Course Content/ Syllabus
1. Isolation ,estimation and electrophoresis of DNA from organisms
2. Isolation of plasmid and quantification
3. Isolation of RNA and quantification
4. Restriction digestion
5. Purification of DNA from gel and Ligation
6. Competent cell preparation and Transformation
7. Conjugation in E.coli:Phage infection in E.coli
8. Induction of Lac operon.
9. Protein Isolation and estimation from microbes
10. Separation of lymphocytes from blood and staining of blood cells
11. Antigen antibody interaction-Haemagglutination, Immunodiffusion,
Immunoelectrophorosis
12. Immunoprecipitation
13. Enzyme linked Immunosorbant Assay(ELISA)
14. Isolation of Immunoglobulins and quantification
15. Western blotting

References:
1. Practical immunology-Frank C HayandOlwyn M R Westwood Blackwell science

18
 2. Manual of immunological methods- Pauline Brousseau.

Internal Continuous Assessment (Maximum Marks-100)

i) Practical Records /outputs – 40 Marks

ii) Regular Class Viva-Voce- 20 Marks
iii) Final Test (Objective)– 40 Marks

Course No: 07BT6215

Course Title:INTRODUCTION TO SEMINAR

Credits: 0-0-1: 0

Year: 2015

Pre- requisites: Nil

Course Objectives:

 To improve the debating capability of the student to present a technical topic

 To impart training to the student to face audience and present his/her ideas and
thus instill self esteem and confidence essential for an engineer.

Outline:

Individual students are required to choose a topic of their interest and give a seminar on that

topic forabout 30 minutes. A committee consisting of at least three faculty members shall

assess the presentationof the seminar. The committee will provide feedback to the students

about the scope for improvements incommunication, presentation skills and body language.

Each student shall submit one copy of the reportof the seminar topic.

Course Outcomes:

 The graduate will have improved the debating capability and presentation

skills in anytopic of his/her choice.

19
SECOND SEMESTER

20
Course No: 07BT6202
Course Title : ANIMAL& PLANT CELL CULTURE
Credits: 4-0-0:4
Year: 2015
Pre-requisites: Nil
Course Objectives:
 An introduction to plant and animal cell tissue culture techniques, nucleic acid
transfections and industrial applications of cell culture.

Course Content/ Syllabus:

Plant tissue culture techniques, preparation of tissue culture media, gene transfer method in
plants, plant regeneration from embryo, meristem and callus culture. Androgenesis: Anther
and pollen culture history of animal cell culture development, Different type of cell culture
media, cell lines, characterization and maintenance of cell lines Animal tissue and Organ
culture- Products and their Applications.

Course Outcome:
1.Describe how cell culture can be used for in vitro studies and commercial applications.
2.Identify the problems associated with growing, storing and identifying a wide range of
different cell types.
Text Books:
1. Dodds J .H. PlantGenetic Engineering, Cambridge University Press.
2. Freshney RI. 2005. Culture of Animal Cells. Wiley Liss.
3. Portner R. 2007. Animal Cell Biotechnology. Humana Press

References: _
1. Mantal S.H., Mathews J.A.Mickee R.A. Principles of Plant Biotechnology An
Introduction to Genetic Engineering in plants, Blackwell Scientific
Publications
2. Bernur R. Pastrnek. J.J. , Molecular Biology, Principles and Applications
in recombinant DNA, Panima Publishing Cooperation, New Delhi

21
 COURSE PLAN
COURSE NO: 07BT6202
COURSE TITLE: ANIMAL & PLANT CELL CULTURE
(L-T-P : 4-0-0) CREDITS:4

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Fundamentals of plant tissue culture, plant

regeneration: organogenesis. Somatic embryogenesis; somaclonal
variation, its genetic basis and application in crop improvement.
Cell/callus line selection for resistance to herbicide, stress and 10 15
diseases. Role of tissue culture in rapid clonal propagation,
production of pathogen - free plants and "synthetic seeds"; haploid
production: advantages and methods.

MODULE: 2Protoplast technology: isolation, culture and plant

regeneration, protoplast fusion, identification and
characterization of somatic hybrids, applications of protoplast
technology. Specific gene transfer: indirect and direct methods, 8 15
current status and limitations. Automation in plant tissue culture.
Field techniques for propagation of regenerated plants

FIRST INTERNAL TEST

MODULE : 3Explant selection, sterilization and inoculation;

Various media preparations; MS, B5, SH PC L-2; Callus and cell
suspension culture; Induction and growth parameters;
Chromosomal variability in callus culture. Plant regeneration 8 15
from embryo, meristem and callus culture. Androgenesis: Anther
and pollen culture; Isolation and culture of protoplasts. Vectors in
plant biotechnology

MODULE : 4Introduction, importance, history of animal cell

culture development, different tissue culture techniques including
primary and secondary culture, continuous cell lines, suspension 10 15
culture, organ culture etc. Different type of cell culture media,
growth supplements, serum free media balanced salt solution,
other cell culture reagents.

22
 SECOND INTERNAL TEST

MODULE: 5Culture of different tissues and its application.

Behavior of cells in culture conditions, division, their growth
pattern, metabolism of estimation of cell number, Development 10 20
of cell lines, characterization and maintenance of cell lines, stem
cells, cryopreservation, common cell culture contaminants.

MODULE: 6Animal tissue and Organ culture- Plasma clot

method, Raft method, Agar-gel method, Grid method, etc. Cyclic
exposure to Medium and Gas phase, Advantages, limitations and 10 20
applications, artificial skin. Products and their Applications,
Transgenics and Prospectives, Principles of invitro fertilization

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT6204

Course Title:ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Integrate the theory and practice for analyzing and quantitating biomolecules
obtained from various sources

Course Content/ Syllabus:

Development and application of Modern analytical instrumentation, Protein separation
techniques and its detection methods .Hybrid techniques, Immunoassay, Advanced analytical
techniques, Lab on chip

23
Course Outcome:
1. Explain the working of modern analytical instruments and use them effectively in their
research work.
2. Instrumentation in quality assessment of biological and pharmaceuticals

Text Books:
1. Practical Biochemistry – Wilson and Walker.
2. Handbook of analytical separations, vol. 4, byIanWilson,2003
3. Encyclopedia of spectroscopy and spectrometry, vol. 1-3,2000
4. Methods ofbiochemicalAnalysis,Vol.35,ClarenceSuelter,1991
5. MethodsofbiochemicalAnalysis,Vol.36,ClarenceSuelter,199

References:
1. Understanding NMR Spectroscopy by James Keeler.
2. Basic One- and Two-Dimensional NMR Spectroscopy Paperback – Import, 27 Oct
2010by Horst Friebolin
3. Fundamentals of Microfluidics and Lab on a Chip for Biological Analysis and Discovery
By Paul C.H. Li.

COURSE PLAN
COURSE NO: 07BT6204
COURSE TITLE: ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1Development and application of Modern analytical

instrumentation. Electrophoresis, Chromatography: HPLC and 6 15
detection methods

MODULE: 2UPLC, GC, HPTLC, Ion exchange

chromatography, gel filtration chromatography and detection 6 15
methodologies.

FIRST INTERNAL TEST

MODULE : 3Hybrid techniques: Gas chromatography with

Fourier transforms infra red spectroscopic detection(GC-FTIR), 8 15
gas chromatography with mass spectrometric detection(GC-MS)

24
MODULE: 4Liquid chromatography with mass spectrometric
detection(LC-MS and LC-MS/MS),andinductively coupled 10 15
plasma with mass spectrometric detection(ICP-MS).

SECOND INTERNAL TEST

MODULE: 5Immunoassay: radioimmunoassay (RIA); enzyme-

multiplied immunoassay technique (EMIT); fluorescence
polarization immunoassay(FPIA); closed enzyme donor 6 20
immunoassay (CEDIA);enzyme-linked immunosorbent assay
(ELISA).

MODULE : 6Advanced analytical techniques : Application of

IR and NMR spectroscopy, Mass spectrometry , Lab on chip 6 20

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT6206

Course Title:TRANSPORT PHENOMENA IN BIOPROCESS SYSTEM
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To understand the balance between momentum, energy and mass in bioprocess
systems

Course Content/ Syllabus:

Introduction to Transport Phenomena -Fermentation Broth Rheology-flow regimes in a
bioreactor- basic concepts of heat transfer and its application in bioconversions-basic
concepts of diffusion-role of diffusion in bioprocessing-Oxygen transport to microbial
cultures-determination of oxygen mass transfer coefficient by various method.

25
Course Outcome:
1. Ability to analyze industrial problems along with appropriate approximations and
and boundary conditions.

Text Books:

1. Pauline M. Doran, Bioprocess Engineering Principles, Academic Press, 1995.

2. Blanch H.W and Douglas S. C, Biochemical Engineering, CRC Press, 1997.
3. Michael L Shuler and FikretKargi, Bioprocess Engineering: Basic Concepts,
Prentice-Hall of India Pvt Ltd, 2008.
References:

1. Arthur T. Johnson, Biological Process Engineering: An Analogical Approach to Fluid

Flow, Heat Transfer, and Mass Transfer Applied to Biological Systems, John Wiley and
Sons, 1998.

COURSE PLAN
COURSE NO: 07BT206
COURSE TITLE: TRANSPORT PHENOMENA IN BIOPROCESS SYSTEM
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

hours Marks;%

MODULE : 1Introduction to Transport Phenomena – momentum,

heat and mass transfer in bioprocessing Review of basic concepts –
Conservation of Mass, Conservation of Energy, Momentum Balance 6 15
– Momentum Balance in a Circular Pipe, Flow Velocity Profile

MODULE : 2Fermentation Broth Rheology – Viscosity,

Rheological Properties of Fermentation Broths, Factors affecting 8 15
broth viscosity Mixing in a Bioreactor – Flow regimes with and
without baffles, various types of impellers and mixing equipment.

FIRST INTERNAL TEST

MODULE: 3Review of basic concepts – Various Modes of heat

transfer, viz., conduction convection and radiation. Calculation of 8 15
Heat-Transfer Coefficients. Application of heat transfer in
bioprocessing,

MODULE : 4Heat Management in Bioreactors, Relationship

between heat transfer, cell concentration and stirring conditions 8 15

26
Review of basic concepts – Diffusivity, theory of diffusion, analogy
between mass, heat and momentum transfer, role of diffusion in
bioprocessing.

SECOND INTERNAL TEST

MODULE: 5Definition of binary mass transfer coefficients.

Convective mass transfer – Liquid-solid mass transfer, liquid-liquid 6 20
mass transfer, gas liquid mass transfer. Oxygen transport to microbial
cultures – Gas liquid mass transfer fundamentals.

MODULE: 6Oxygen requirement of microbial cultures. Oxygen

transfer by aeration and agitation. Determination of oxygen mass 6 20
transfer coefficient by various methods including dynamic gassing
out and oxygen balance methods.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No:07BT6212
Course Title:SEMINAR
Credits: 0-0-2:2
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To assess the debating capability of the student to present a technical topic.Also to
impart training to a student to face audience and present his ideas and thus creating
in him / herself esteem and courage that are essential for an engineer.

Outline

 All students are required to choose a topic of their interest from Industrial
Biotechnology and its applications related topics preferably from outside the M.Tech
syllabus and give a seminar on that topic about 30 minutes. A committee consisting
of at least three faculty members shall assess the presentation of the seminar and

27
 award marks to the students. Each student shall submit two copies of a write up of
his / her seminar topic. One copy shall be returned to the student after duly certifying
it by the Chairman of the assessing committee and the other will be kept in the
departmental library. Internal continuous assessment marks are awarded based on the
relevance of the topic, presentation skill, quality of the report and participation.

 Internal continuous assessment: 100 marks

Marks for the report: 30%

Presentation: 40%
Viva Voce: 30%

Course No: 07BT6214

Course Title: MINI PROJECT
Credits: 0-0-4: 2
Year: 2015
Pre-requisites: Nil
Course Objectives:

 To estimate the ability of the student in transforming the theoretical knowledge

studied so far into an application at the basic research level that could lead to an
industrial application. For enabling the students to gain experience in organisation
and implementation of a small project and thus acquire the necessary confidence to
carry out main project in the final year. The basic concepts of product design may be
taken into consideration while designing the project. A committee consisting of
minimum three faculty members specialised in biotechnology/chemical
engineering/instrumentation engineering will perform assessment of the mini project.
Students have to submit a report on the mini project and demonstrate the mini project
before the evaluation committee.

 Internal continuous assessment: 100 marks

Marks for the report: 20%

Demonstration and Presentation: 50%
Results: 30%

28
Course No: 07BT6216
Course Title: BIOPROCESS & FERMENTATION TECHNOLOGY LAB
Credits: 0-0-2:2
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To understand and apply the basic and advanced concepts of fermentation and bioprocess in
industry
Course Content/ Syllabus:
1. Isolation of industrially important microbes (Bacteria, Actinomycetes&Fungi) from
environment
2. Identification and Culture preservation of industrially important microbes
a. Staining techniques (Gram staining &Fungal staining)
b. Glycerol stock preparation
3. Production of extracellular enzyme by liquid fermentation and Quantification of enzyme
activity and specific activity
4. Kinetics study of enzymes
5. Techniques of enzyme immobilization
6. Production of metabolites by solid state fermentation
7. Strain improvement by non recombinant methods-Physical mutation and chemical mutation
8. Experimental design for improvement of fermentation by Plackett-Burman method
9. Study of Rheology of fermentation broth
10. Determination of volumetric mass transfer coefficient by sodium sulphite oxidation method.
11. Down stream processing :a. Cell rupture ,b. Precipitation,c. Dialysis,d. Chromatography
e. Molecular weight determination by SDS PAG

References:
1. Practical Fermentation Technology- Brian McNeil, Linda M.Harvey- John Wiley &
Sons, Ltd

2. Enzyme Technology Martin F. Chaplin , Christopher Bucke

Internal Continuous Assessment (Maximum Marks-100)

i) Practical Records /outputs - 40%

ii) Regular Class Viva-Voce- 20%
iii) Final Test (Objective) -40%

29
THIRD SEMESTER

30
Course No:07BT7205
Course Title:SEMINAR
Credits: 0-0-2:2
Year: 2015
Pre-requisites: Nil
Course Objectives:

 To assess the debating capability of the student to present a technical topic.Also to

impart training to a student to face audience and present his ideas and thus creating
in him / herself esteem and courage that are essential for an engineer.

 All students are required to choose a topic of their interest from Biotech Business
and entrepreneurship development related topics preferably from outside the
M.Tech syllabus and give a seminar on that topic about 30 minutes. A committee
consisting of at least three faculty members shall assess the presentation of the
seminar and award marks to the students. Each student shall submit two copies of a
write up of his / her seminar topic. One copy shall be returned to the student after
duly certifying it by the Chairman of the assessing committee and the other will be
kept in the departmental library. Internal continuous assessment marks are awarded
based on the relevance of the topic, presentation skill, quality of the report and
participation.

 Internal continuous assessment: 100 marks

Marks for the report: 30%

Presentation: 40%

Viva Voce: 30%

31
Course No:07BT7207
Course Title:PROJECT [PHASE 1]
Credits: 0-0-12: 6
Year: 2015

Course Objective:

 To improve the professional competency and research aptitude by touching the areas
which otherwise not covered by theory or laboratory classes. The project work aims
to develop the work practice in students to apply theoretical and practical
tools/techniques to solve real life problems related to industry and current research.

Outline
The project work can be a purely research based / industry based project which contains and
applies the basic and advanced principles of biotechnology as well as chemical engineering.
The project work is allotted individually on different topics. The students shall be encouraged
to do their project work in the parent institute itself. If it is found essential, they may be
permitted to continue their project outside the parent institute subject to the conditions in
clause 10 of M.Tech regulations. Department will constitute an Evaluation Committee to
review the project work. The Evaluation committee consists of at least three faculty members
of which internal guide and another expert in the specified area of the project shall be two
essential members.
The student is required to undertake the Masters research project phase-I during
the third semester and the same is continued in the 4th semester (Phase-II). Phase-I consists of
preliminary thesis work, two reviews of the work and the submission of preliminary report.
First review would highlight the topic, objectives, methodology and expected results. Second
review evaluates the progress of the work, preliminary report and scope of the work which is
to be completed in the 4th semester.

 Internal continuous assessment :Marks 50

Progress evaluation by the Project Supervisor: 20 Marks

Presentation and evaluation by the Committee: 30 Marks

32
FOURTH
SEMESTER

33
Course No:07BT7202
Course Title:PROJECT [PHASE 2]
Credits: 0-0-12:12
Year: 2015

Course Objective:
 To improve the professional competency and research aptitude by touching the areas

which otherwise not covered by theory or laboratory classes. The project work aims

to develop the work practice in students to apply theoretical and practical

tools/techniques to solve real life problems related to industry and current research.

Outline
Masters Research project phase-II is a continuation of project phase-I started in the third
semester. Before the end of the fourth semester, there will be two reviews, one at middle of
the fourth semester and other towards the end. In the first review, progress of the project
work done is to be assessed. In the second review, the complete assessment (quality, quantum
and authenticity) of the Thesis is to be evaluated. Both the reviews should be conducted by
guide and Evaluation committee. This would be a pre qualifying exercise for the students for
getting approval for the submission of the thesis. At least one technical paper is to be
prepared for possible publication in journal or conferences. The technical paper is to be
submitted along with the thesis. The final evaluation of the project will be external
evaluation.

 Internal Continuous Assessment: 100 marks

Project evaluation by the Supervisor/s : 30 Marks

Presentation & evaluation by the Committee : 40 Marks
Evaluation by the External expert : 30 Marks

34
ELECTIVES

35
ELECTIVE I
07BT6209X

36
Course No: 07BT62091
Course Title:CLINICAL BIOTECHNOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To understand the design of Clinical trails and research
 To know about some of the important biological , their use and applications in the
pharmasector

Course Content/ Syllabus:

Clinical study and design of clinical studies.Epidemiological research and treatment studies.
Observational studies: Cohort study- Prospective cohort and Retrospective cohort.
Community survey and Ecological study. Seasonal studies. Statistical Analysis and
Interpretation:Drug Design and Synthesis. Statistical Application Software.Study of
Therapeutic Proteins and Related Case Studies.Cancer Biology and Therapy Clinical
Toxicology.Clinical Research Governance and Ethics.

Course Outcome:
1. Design and carry out clinical studies
2. Statistical analysis and Interpretation of clinical data
Text Books:

1. Pharmaceutical Biotechnology, Second Edition by Michael J. Groves

2. Medical Biotechnology by JuditPongracz, Mary Keen (2009)
3. Medical Biotechnology by FirdosAlam Khan (2012)

References:
1. Leon Lachmanetal, Theory and practice of Industrial Pharmacy, Lea arid Febiger.
2. Richard B. Silverman, The Organic Chemistry of Drug Design and Drug Action,
Elsevier, Publications.
3. Rang Dale Riter, Pharmacology, Churchill Livingstone

37
 COURSE PLAN
COURSE NO: 07BT62091
COURSE TITLE: CLINICAL BIOTECHNOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE: 1Introduction to Clinical study and design of
clinical studies. Epidemiological research and treatment studies:
Double-blind and Single-blind Randomized controlled trial, 5 15
Non-blind trial, Nonrandomized trial-quasi-experiment.

MODULE: 2 Observational studies: Cohort study- Prospective

cohort and Retrospective cohort. Time series study, Case-control
study and Nested case-control study. Community survey and 5 15
Ecological study. Seasonal studies: Conduction of studies in
seasonal indications such as Allergies and Influenza.

FIRST INTERNAL TEST

MODULE: 3 Statistical Analysis and Interpretation: Background

and purpose, trial design consideration, Parallel group design,
cross over design, factorial design. Introduction to Statistical 8 15
Application Software (SAS), procedures and clinical data
management.

MODULE: 4 DrugDesign and Synthesis: Synthesis of

compounds in accordance with the molecular structure and
biological activity concept: Analgesics, neuromuscular blocking 6 15
agents, anti-fertility drugs and bactericidal & bacteriostatic agents
(sulphonamides, mercury compounds and antiseptics).

SECOND INTERNAL TEST

MODULE: 5 Clinical Toxicology: Basic concept in toxicology.

Types and mechanism of toxin action- Epoxidation& drug
toxicity, N-oxidation & drug toxicity and sulphurxenobiotics. 10 20
Hepatotoxicity and Nephrotoxicity. Biotransformation of toxins,
inactivation and removal from the body. Blood bags, storage of
blood

MODULE: 6 Clinical Research Governance and Ethics:

Overview on regulatory affairs for pharmaceuticals,
neutraceuticals and medical devices. Good Clinical Practices 8 20
(GCP).
International quality standard and related guidelines (ICH-E6).
Risk assessment and trial monitoring. Legal and ethical issues on
biotechnology, medical research and related clinical practice.

END SEMESTER EXAMINATION

38
ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62093

Course Title:BIOPHARMACEUTICAL& PHARMACEUTICAL TECHNOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Prepare individuals for employment in pharmaceutical manufacturing and related
industries
Course Content/ Syllabus:
Pharmaceuticals, biologics and
biopharmaceuticals.Pharmacokinetics.ADME.Biopharmaceuticals,pharmaceutical
biotechnology. Preclinical Pharmacokinetics .CompartmentModeling.Pre-clinical trials,
Clinical trials.Patenting- Patent types.Special Concerns for the Preclinical Evaluation of
Biotechnology Products.The drug development and manufacturing process.Regulations for
Industries.

Course Outcome:
1. To get acquainted with the basic concepts in pharmaceuticals, biologics and
biopharmaceuticals
2. To understand the details about pharmacokinetics
3. Acquire sufficient knowledge on the functioning of pharma and biopharma industries
Text Books:

1. Heinrich Klefenz ,Industrial pharmaceutical biotechnology, John Wiley sons, 2002.

2. Susanna Wu-Pong, YongyutRojanasakul, and Joseph Robinson, Biopharmaceutical
drug and design and development, Humana Press, 2007.
3. Gary Walsh, Biopharmaceuticals: Biochemistry and Biotechnology (2e), John Wiley
& Sons, 2003.

39
 References:
1.Herbert A Kirst, Wu-KuangYeh; Milton J, Enzyme Technologies for
pharmaceutical andbiotechnological applications, WILEY-VCH Verlag, 2003.

COURSE PLAN

COURSE NO: 07BT62093

COURSE TITLE:BIOPHARMACEUTICAL & PHARMACEUTICAL TECHNOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Introduction to pharmaceutical products,

Biopharmaceuticals and pharmaceutical biotechnology, 7
Biopharmaceuticals: current status and future, Basic Issues in the 15
Manufacture of Macromolecules, Traditional pharmaceuticals of
biological origin-animal origin, plant origin, microbial origin.

MODULE: 2 Pharmacokinetics: Pharmaco-Kinetics- absorption of

drugs, distribution of drugs, protein binding of drugs, 8 15
Biotransformation of drugs, bioavailability and bioequivalence,
excretion of drugs

FIRST INTERNAL TEST

MODULE: 3 Pharmacokinetics – effects of food and fasting.

Preclinical Pharmacokinetics 6 15
Compartment Modeling- one compartment open Model, two
compartment open Model, multi compartment Model, non linear
kinetics.

MODULE: 4 The drug development and manufacturing process:

Drug discovery, Pre-clinical trials, Clinical trials. Patenting- Patent 6 15
types, The patent application, Patenting in biotechnology,.

SECOND INTERNAL TEST

MODULE: 5 Special Concerns for the Preclinical Evaluation of

Biotechnology Products, Immunotoxicology in biopharmaceutical 7 20
development, formulation and Delivery of Therapeutic Proteins,
Stability Testing, Filling, and Packaging.

40
MODULE: 6 Regulations for Industries: The role and remit of
regulatory authorities-The Food and Drug Administration, The 8 20
investigational new drug application, The new drug application,
European regulations, WHO good manufacturing practices: main
principles for pharmaceutical products. Hazard and risk analysis in
pharmaceutical products.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62095

Course Title:BIOPOLYMER TECHNOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Altering the molecular structure of polysaccharides by enzymatic and chemical means
 Help in offering solutions towards technology development for biodegradable
polymers

Course Content/ Syllabus:

Biopolymers – The current scenario,Biopolymers and Artificial Biopolymers in Biomedical
Applications, an Overview, Novel Synthesis. Preparation and Characterizations, Composite
Materials Based on Gelatin and Fillers from Renewable Resources. Biosynthesis and
Modifications of xanthum gum, PHA, PHB etc. Bio-surfactants: Source, characteristics and
properties. Production of Bio-surfactants.Material Testing and Analytical Methods.Case
studies.

Course Outcome:
1. To understand the basic concepts about biopolymers.
2. To familiarise with Biopolymer Technology and Applications
Text Books:

41
 1. EmoChiellini ,EmoChiellini and Helena Gil, Biorelated Polymers: Sustainable
Polymer Science and Technology, Springer 2001.
2. Johnson .R.M, L.Y. Mwaikambo and N. Tucker, Biopolymers, Rapra Technology,
2003.
References:
1. NaimKosaric(Ed). Biosurfactants.Marcell Dekker Inc, 1993.
COURSE PLAN

COURSE NO: 07BT62095

COURSE TITLE:BIOPOLYMER TECHNOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Introduction: Biopolymers – The current 6 15

scenario, different biopolymers – produced from various
renewable resources, characteristics, merits and demerits over
conventional polymers.

MODULE: 2 Biopolymer Technology and Applications:

Biopolymers and Artificial Biopolymers in Biomedical 6 15
Applications, an Overview, Novel Synthesis of Biopolymers
and Their Medical Applications, Composite Films Based on
Poly (Vinyl alcohol) and LignocellulosicFibres:

FIRST INTERNAL TEST

MODULE: 3 Preparation and Characterizations, Composite

Materials Based on Gelatin and Fillers from Renewable
Resources: Thermal and Mechanical Properties, Properties of 8 15
PHAs and Their Correlation to Fermentation Conditions.

MODULE: 4 Biosynthesis and Modifications: Synthesis and

Modification of different Biopolymers like xanthum gum, PHA,
PHB etc. Polymers for storing biological molecules (blood 6 15
bags).

SECOND INTERNAL TEST

MODULE : 5 Bio-surfactants: Source, characteristics and

properties of Bio-surfactants; Production of Bio-surfactants via
the fermentation and biotransformation routes; Production of 8 20
Bio-surfactants with immobilized cells; Integrated bioprocess
for continuous production of Bio-surfactants including
downstream processing; Applications of Bio-surfactants – Food
Industry, Environmental Control.

42
MODULE: 6 Material Testing and Analytical Methods:
An Overview of Available Testing Methods, Comparison of
Test Systems for the Examination of the Fermentability of
Biodegradable Materials, Structure-Biodegradability 8 20
Relationship of biopolymers Case studies.Optimization of
production and purification of Xanthum gum and other
biopolymers like PHA, PHB.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62097

Course Title:PROTEIN ENGINEERING
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Knowledge of structure and function of amino acids , protein structure

Course Objectives:
 Learn structure and function of proteins of particular importance, the student will
know the production of recombinant insulin & in general how to engineer protein to
be used as therapeutics

Course Content/ Syllabus:

The protein makeup. Interaction and elucidation of protein structure with electromagnetic
radiation. Peptide mapping, peptide sequencing, significance of Ramachandran’splot.High-
throughput protein sequencing setup. Zn fingers, helix-turn helix motifs in homeodomain,
Leucine zippers. Membrane proteins.Design and construction of novel proteins and enzymes,
Conformation of proteins in general and enzymes in particular.Effect of amino acids on
structure of proteins, Energy status of a protein molecule.

Course Outcome:

43
 1. To have a deeper understanding of thephysico chemical make up of proteins.
2. To understand the structure function relationship between proteins

Text Books:
1. Voet D. and Voet G., “Biochemistry”, Third Edn. John Wiley and Sons, 2001
2. Creighton T.E. Proteins, Freeman WH, Second Edition, 1993

References:
1. Branden C. and Tooze J., “Introduction to Protein Structured, Second Edition”,
Garland Publishing, NY, USA, 1999
2. Moody P.C.E. and Wilkinson A.J. “Protein Engineering”, IRL Press, Oxford, UK,
1990.

COURSE PLAN
COURSE NO: 07BT62097
COURSE TITLE:PROTEIN ENGINEERING
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Protein interactions: Covalent, Ionic, Hydrogen,

Coordinate, hydrophobic and Vander vals interactions in protein
organization. Interaction and elucidation of protein structure with 5 15
electromagnetic radiation. Peptide mapping, peptide sequencing,
significance of Ramachandran’s plot.

MODULE: 2 High-throughput protein sequencing setup

Secondary structures: Alpha, beta and loop structures and methods
to determine. Super-secondary structure: Alpha-turn-alpha, beta-
turn-beta (hairpin), beta-sheets, alpha-beta-alpha, topology 6 15
diagrams, up and down & TIM barrel structures nucleotide
binding folds, prediction of substrate binding sites. Tertiary
structure: Domains, folding, denaturation and renaturation,
overview of methods to determine 3D structures, Quaternary
structure: Modular nature, formation of complexes.

FIRST INTERNAL TEST

MODULE: 3 Structure function relationship: DNA-binding 5 15

proteins: prokaryotic transcription factors, Helix-turn-Helix
motif in DNA binding, Trp repressor, Eukaryotic transcription
factors, Zn fingers, helix-turn helix motifs in homeodomain,
Leucine zippers.

MODULE: 4 Membrane proteins: General characteristics,

Transmembrane segments, prediction, bacteriorhodopsin and

44
Photosynthetic reaction center, Immunoglobulins: IgG Light chain 15
and heavy chain architecture, abzymes and Enzymes: Serine 6
proteases, understanding catalytic design by engineering trypsin,
chymotrypsin and elastase, substrate-assisted catalysis other
commercial applications.

SECOND INTERNAL TEST

MODULE: 5 Design and construction of novel proteins and

enzymes, Physical methods such as x-ray crystallography for
determination of protein structure, Site directed mutagenesis for 10 20
specific protein function, Basic concepts for design of a new
protein/enzyme molecule.

MODULE : 6Protein phosphorylation – immunoglobulins -

Nucleotide binding proteins – enzyme serine proteases -
ribonuclease – lyzozyme ,methods to alter primary structure of 10 20
proteins –Engineered proteins – de novo protein design.
Therapeutics - cellular and molecular therapeutics, Interferons,
insulin, monoclonal antibodies in therapy.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

45
ELECTIVE II
07BT6208X

46
Course No: 07BT62082
Course Title:BIOPROCESS MODELS: DESIGN AND STABILITY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Knowledge of MATLAB
Course Objectives:
 Develop knowledge and appreciation of the conceptual and factual bases for
bioprocess design and operation

Course Content/ Syllabus:

Aspects of Modeling in bioprocess industries-Uses and limitations of mathematical
Models.Importance of probability and Stochasticity in biological process. Characteristics of
a dynamical system:Monad Model, Lotka-Volterra systems. Numerical methods for solving a
linear system:Development of mathematical Models:Analysis of mathematical
Models:Concepts of stability in multidimensional systems - Equilibrium points, limit
cycles, Eigenvalues of the Jacobian matrix

Course Outcome:
1. Ability to solve optimization using MATLAB.

2. Application in process development

Text Books:

1. Wayne Bequette.B, Process dynamics Modeling and analysis and simulation,.

PrenticeHallInc, 2004.
2. John H. Seinfeld and Leon Lapidus., Mathematical Methods in Chemical Engg., (Vol.
3),Process Modeling, Estimations and Identification. Prentice Hall, 1974.
3. Volesky.B and J. Votruba., Modeling and Optimization of Fermentation Process
(Process Simulation and Modeling).Elsevier Science and Technology, 1992.
References:
1.Dynamic Moduleels in Biology By Stephen P. Ellner, John Guckenheimer.

2. Katok, A. B. and B. Hasselblatt (1999). Introduction to the Modern Theory of

47
 Dynamical Systems. Cambridge, CambridgeUniversity Press
3. Izhikevich E.M. (2007) Dynamical Systems in Neuroscience:
The Geometry of Excitability and Bursting. The MIT Press, Cambridge, MA
COURSE PLAN
COURSE NO: 07BT62082
COURSE TITLE: BIOPROCESS MODELS: DESIGN AND STABILITY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE : 1Aspects of Modeling in bioprocess

industries: Basic classifications, fundamental features
of Models: Knowledge-based and data based Models,
unstructured and structured Models, compartmental Models, 8 15
metabolic network Models, fuzzy logic, hybrid Models

MODULE : 2Uses and limitations of mathematical Models :

Illustrations of algebraic equations, ordinary differential
equations, difference equations partial differential equations,
integral equations and integro-differential equations. 6 15
Importance of probability and Stochasticity in biological
processes. Introduction to chaos

FIRST INTERNAL TEST

MODULE: 3Characteristics of a dynamical system: State-

space representations: state variables and parameters, input and
output vectors, state evolution. Linear and non-linear systems. 6 15
Popular examples like Monad Model, Lotka-Volterra systems

MODULE: 4 Numerical methods for solving a linear

system:General form of a linear system , Concepts of numerical
techniques : Newton's method, Euler Integration, Runge-Kutta 6 15
Integration, MATLAB integration routines. Linearization of
non-linear Models and its interpretation: basic concepts

SECOND INTERNAL TEST

48
MODULE : 5Development of mathematical Models:Material
and energy balances - Design parameters and tuning parameters
- developing equations for process rates from first
principles.Concepts in optimization for parameter estimation:
Objective function, convex problems, parameter search space -
local and global optimums, stationary points, Hessian matrix, 10 20
Necessary and sufficient conditions for
optimality, constraints, Lagrange multipliersDesign of
experiments for parameter estimation - Accuracy of parameter
estimates: sensitivity analysis. Concepts in parameter
identifiability

MODULE : 6Analysis of mathematical Models:Concepts of

stability in one dimensional systems - Equilibria, convergence,
eigenvalues, attractors and basin of attraction, bistability,
phase portraits, bifurcation Concepts of stability in 6 20
multidimensional systems - Equilibrium points, limit
cycles, eigenvalues of the Jacobian matrix.

END SEMSETER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62084

Course Title:FOOD PROCESSING TECHNOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Understand the basics of manufacturing of food products of consistent quality and
nutritional value at affordable cost
 Develop skilled and competent manpower for food processing industries

Course Content/ Syllabus:

49
Food as Source of Nutrients - Effect of processing on food proteins, Food additives.food
microbiology-preservation techniques-Food storage Food packaging-Units Operations in
Food Processing Industry. Microbial spoilage and methods of control of fruits and vegetables.
Factors causing food spoilage, Food Preservation Hydrothermal treatment of grains-concepts
of Food Quality-Quality assurance

Course Outcome:
1. Explain the spoilage and deterioration mechanisms in foods and methods to control
deterioration and spoilage
2. Describe the transport processes and unit operations in food processing as
demonstrated both conceptually and in practical laboratory settings.
3. Explain the properties and uses of various packaging materials.
4. An updated knowledge about maintenance of food quality control aspects

Text Books:
1. Food Chemistry by L H Meyor (CBS Publisher, Delhi)
2. Modern Food Microbiology, James M. Jay, CBS Publishers & Distributors, Delhi.
3. Food Engineering Fundamentals, J.Clair Batty, Steven L Folkman, John Wiley&
Sons.
4. Fundamentals of Food Process Engineering, Romeo Toledo, Van NostrandReinhold,
New York

References:
1. Food Microbiology, W C Frazier and D C Westhoff, McGraw Hill Book Company,
NY.
2. Post harvest technology of Cereals, Pulses and Oilseeds by Chakravarti A. Oxford
Publishing.
3. Cereal Technology by Potter NN. AVI Publication.
4. Unit Operations of Agricultural Processing, K.M.Sahay&K.K.Singh, VikasPublishing
House.
5. Engineering of Dairy & Food Products, A.W.Farral.

COURSE PLAN

50
 COURSE NO: 07BT62084
COURSE TITLE: FOOD PROCESSING TECHNOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE : 1 Food as a Source of Nutrients: sources and
function of lipids, carbohydrates, proteins and minerals. Effect of 6 15
processing on food proteins, Food additives.

MODULE: 2 Significance of microorganisms in foods.

Chemical & Microbial Kinetics in Food Products, Nutrient
preservation. Food packaging materials. Introduction to Units 6 15
Operations in Food Processing Industry, Radiation sensitivity of
micro-organisms, Effect of ionizing radiation on nutrients

FIRST INTERNAL TEST

MODULE: 3 Food storage requirements and methods.

Microbial spoilage and methods of control of fruits and 6 15
vegetables. Pasteurization of milk and defects in milk and milk
products. Microbiology of Canned Foods

MODULE: 4 Hydrothermal treatment of grains, Parboiling and

milling of paddy,Dry and wet milling of corn, Modern methods 6 15
of milling of pulses. Factors affecting milling of pulses

SECOND INTERNAL TEST

MODULE: 5 Engineering Properties of Food Materials:

Mechanical, Physical &Rheological properties. Food Spoilage & 8 20
Control: Factors causing food spoilage, Food Preservation by use
of Low Temperature, High temperature and Drying

MODULE: 6 General concepts of Food Quality, Safety and

Management Systems, General principles of food hygiene – 10 20
Quality assurance: GPs, HACCP system for food quality, ISO .
Case studies.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial

51
 End Semester Examination 60

Course No: 07BT62086

Course Title:METABOLIC ENGINEERING
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To understand and utilize cellular pathways for chemical transformation, energy
transduction, and supramolecular assembly
 Purposeful alteration and manipulation of metabolic pathways for industrial
applications.
Course Content/ Syllabus:
Prokaryotic and Eukaryotic cell structures; pure culture techniques-Different media (simple,
complex and defined) - Growth curve-Bioenergetics, Metabolism of Biomolecules-,
Metabolic engineering in practice: Concept of directed cellular energy utilization –analytical
and synthetic elements of metabolic engineering-Application of pathway manipulations

Course Outcome:
1. Have acquired in depth knowledge on metabolic pathways and their regulation and
on how metabolic pathways can be engineered for the improvement of various
bioprocesses.

Text Books:
1. G Stephanopoulos et al; Metabolic Engineering principles & Methodologies
2. T. Scheper R Faurie, J. ThommelAdvance in Biochemical engineering
Biotechnology:Microbila production of L – Aminoacid
References:

1. Microbiology, L.M. Prescott, J.P. Harley and D.A. Klein, 7/e, 2007. McGraw Hill,
Boston.
2. Fundamental Principles of Bacteriology, A.J. Salle, 1999. Tata McGraw - Hill
Publishing Company Limited, New Delhi.

52
 3. Microbial Ecology. Fundamentals and Applications, R. M. Atlas and R. Bartha,
2000.
4. Microbiology, M.J. Pelzer Jr., E.C.S. Chan and N.R. Kreig, 1993. McGraw Hill
Inc., New York.
5. Biochemistry, 4th edition, L.Stryer., 1999. W.H, Freeman & company, New York.
6. Principles of Biochemistry, AL. Lehninger, D.L. Nelson and M. M. Cox., 1993.
Worth Publishers, New York.
7. Biochemistry 4th edition, G. Zubay, 1998. McMillan Publishing Co. New

COURSE PLAN
COURSE NO: 07BT62086
COURSE TITLE: METABOLIC ENGINEERING
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE: 1Prokaryotic and Eukaryotic cell structures; pure culture
techniques- isolation, cultivation, enumeration and preservation of
microbes; staining techniques- simple and differential staining. 15
Nutritional requirements and nutritional grouping of microorganisms; 6
Different media (simple, complex and defined)

MODULE: 2 Growth curve; Axenic culture, Synchronous culture,

Continuous culture; Different; Effects of physical and chemical factors
on microbial growth. Microbial genetics-recombination - 8 15
transformation, transduction, conjugation, regulation of gene
expression. Bioenergetics, Metabolism of Carbohydrates, Proteins,
Amino acids, Lipids and Nucleic acids-their biosynthesis and
degradation

FIRST INTERNAL TEST

MODULE: 3 Integration of carbohydrate and fatty acid metabolism.

Mechanism of oxidative phosphorylation and its inhibitors, and
photophosphorylation, urea cycle, hormonal regulation of mammalian
metabolism. Heterocylic compounds and secondary metabolites - 8 15
prostaglandins, leukotrienes, thromboxanes, interferons and
interleukins, antibodies, alkaloids, plant and animal pigments

53
MODULE: 4 Metabolic engineering in practice: Concept of directed
cellular energy utilization –analytical and synthetic elements of
metabolic engineering – targets of metabolic engineering. Strategies for 15
redirecting branched and linear pathways: (Alteration of feed back 6
regulation; limiting accumulation of end product feed back resistant
mutants, alteration of permeability).

SECOND INTERNAL TEST

MODULE : 5 Metabolic Flux Analysis: Concept and utility of MFA –

Theory – case studies – over determined systems – experimental
determination of MFA by isotope labeling – applications of MFA: Case
studies- concept & fundamentals of metabolic control analysis. 8 20
Application of pathway manipulations: Strategies for overproduction of
primary metabolites.

MODULE: 6 Strategies for overproduction of secondary metabolites

(precursor effects, prophophaseidiophase relationship, enzyme 6 20
induction, feed back regulation.)
Bioconversions: (ME concepts applied in process decisions for enhanced
bioconversion).
Examples of pathway manipulations: Enhancement of product yield
(alcohol, amino acids) – extension of substrate ranges (lignocelluloses
utilization) – extension of product spectrum (antibiotic, biopolymers) -
improvement of cellular properties (alteration of metabolism, enhanced
efficiency and yield, genetic stability).

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62088

Course Title:BIO PROCESS MODELLING AND SIMULATION
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To give student an understanding of Process Modeling and Simulation

Course Content/ Syllabus:

54
Elements of probability theory-Perspective on Modeling of physical, chemical and biological
phenomena-mathematical Models in Bioprocess Model-Mathematical Models for mixing
vessel- Review of numerical techniques for the solution of bioprocess Models.-Introduction
to population balance Modeling in bioprocess engineering.

Course Outcome:
1. Develop model equations for a given system
2. Demonstrate model solving ability for various processes/unit operations
Text Books:
1. John H. Seinfeld and Leon Lapidus., Mathematical Methods in Chemical Engg., (Vol.
3), Process Modeling, Estimations and Identification. Prentice Hall, 1974.
2. Luyben W.L., Process Modelling, Simulation and Control for Chemical Engineers,
McGraw Hill International Edition
References:
1. Ramakrishna. D, Population Balances. Academic Press, 2000

2. Biquette W.B., Process Dynamics - Modeling Analysis and Simulation, Prentice Hall
3. Volesky.B and J. Votruba., Modeling and Optimization of Fermentation Process
(Process Simulation and Modeling). Elsevier Science and Technology, 1992.
4. Biquette W.B., Process Dynamics - Modeling Analysis and Simulation, Prentice Hall
of India.

COURSE PLAN
COURSE NO: 07BT62088
COURSE TITLE: BIO PROCESS MODELLING AND SIMULATION
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE : 1Perspective on Modeling of physical, chemical and

biological phenomena, uses and limitations of mathematical Models in 6 15
Bioprocess Models- Basic classifications, fundamental features of
Models.

MODULE : 2 Examples involving algebraic equations, ordinary

differential equations, difference equations partial differential
equations, integral equations and integro-differential equations. 8 15
Elements of probability theory, stochastic Models parameter
estimation Model forms for parameter estimation.

55
 FIRST INTERNAL TEST

MODULE: 3 Parameter estimation using moments, design of

experiments for parameter estimation. Accuracy of parameter 6 15
estimates. Design of experiments for Model discrimination -
Regression and interpolation

MODULE : 4 Mathematical Models for mixing vessel- mixing

with reaction - reversible reaction- steam jacketed vessel-
isothermal constant and variable hold up CSTR in series- 8 15
Boiling of single component liquid-open and closed vessel -
continuous flow boiling. Multi-component boiling system -
batch distillation-condensation.

SECOND INTERNAL TEST

MODULE: 5 Review of numerical techniques for the solution

of bioprocess Models. Non linear systems analysis Phase –
Plane analysis in classical bioreactor Models, 8 20
Introduction to population balance Modeling in bioprocess
engineering – The solution of population balance equations.

MODULE: 6 Budding of yeast population – Modeling of cells with

dynamic morphology – Modeling for biological populations with 6 20
correlation between life spans of siblings. Modeling of Industrial
sterilization processes

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Internal Exam 2 15
Assessment 10
Assignment/Mini Project/Tutorial
End Semester Examination 60

56
ELECTIVE III
07BT6210X

57
Course No: 07BT62102
Course Title:MOLECULAR DIAGNOSTICS
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Understanding the molecular basis of diseases and techniques involved in diagnosis
of diseases.

Course Content/ Syllabus:

Host pathogen interactions in disease processMolecular techniques for analysis of
Biochemical, Immune, Genetic disorders.Monoclonal antibodies as diagnostic reagents
Development of molecular diagnostic technology.-Molecular techniques for diagnosis-DNA
sequencing and diagnosis ,biosensors and nanotechnology.. OIE guidelines in development of
diagnostics. Present methods for diagnosis of Specific diseases. Current issues and
opportunities in the diagnostic sector

Course Outcome:

1. Describe the laboratory techniques used to detect: microorganisms, inherited disease,

DNA polymorphisms, neoplastic processes, DNA based tissue typing.
2. Outline the quality assurance process required in a molecular diagnostic laboratory.
Text Books:

1. Fundamentals of Molecular Diagnostic Paperback – 1 Jan 2007by Carl A. Burtis , David

Bruns, Edward R. Ashwood

58
2. Tietz Textbook of Clinial Chemistry and Molecular Diagnostics Hardcover – 1 Jan
2012by Carl A. Burtis

References:
1. Elles R &Mountford R. 2004. Molecular Diagnosis of Geneti Disease. Humana Press.
2. Rao JR, Fleming CC & Moore JE. 2006. Molecular Diagnostics Horizon Bioscience.
3. Andrew Read and Dian Donnai, New clinical Genetics, Scion Publishing Ltd,
Oxfordshire, UK, 2007.
4. James W Goding, Monoclonal antibodies: Principles and Practice, 3rd Edition,
Academic Press, 1996.

COURSE PLAN
COURSE NO: 07BT62102
COURSE TITLE: MOLECULAR DIAGNOSTICS
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE : 1Host pathogen interactions in disease process.

Clinical diagnosis of diseases. Introduction and historical 6 15
perspective of development of molecular diagnostic
technology. Cancer biomarkers.

MODULE : 2Concept of development of group specific and

strain specific nucleic acid based diagnostics, basis for
selection of gene/nucleotide sequence of pathogenic organism 6 15
to target for detection. Molecular techniques for analysis of
Biochemical, Immune, Genetic disorders.

FIRST INTERNAL TEST

MODULE : 3Application of restriction endonuclease

analysis for identification of pathogens. Antibody based
diagnosis; Monoclonal antibodies as diagnostic reagents. 6 15
Diagnosis of infectious diseases by using ELISA and Western
blot.

MODULE : 4DNA sequencing and diagnosis , Theoretical

background of development of PCR and Real time PCR and
its variations, application of PCR for diagnosis of infectious 8 15
diseases of animals and poultry, nucleic acid sequence based
diagnostics.

SECOND INTERNAL TEST

59
MODULE : 5Array based techniques in diagnosis (DNA &
Protein array); single nucleotide polymorphism and disease 8 20
association; Two dimensional gene scanning. Diagnostic
technology based by biosensors and nanotechnology.

MODULE : 6OIE guidelines in development of diagnostics. 20

Present methods for diagnosis of Specific diseases like 8
Tuberculosis, Malaria and AIDS; Ethics in Molecular
Diagnosis. Current issues and opportunities in the diagnostic
sector.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62104

Course Title:BIOREACTOR DESIGN
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Learn the basic and advanced principles of a designing a reactor for industrial
applications

Course Content/ Syllabus:

Introduction and Review of Bio-reaction engineering concepts-Modeling of non-ideal
behavior in bio reactors-Continuous Stirred Tank Bioreactor-Fed–batch reactor-Recycle
system-The Transient Behavior of Bioreactors-Design of a fermenter-Instrumentation and
control-structured and unstructured Models for bioprocess systems.

Course Outcome:
1. Estimate values for process variables using suitable correlations and equations
developed from first principles.

60
 2. Formulate sensitivity analysis on bioreactor systems and determine optimum
operating parameters.
Text Books:

1. Chemical Reaction Engineering - Octave Levenspiel

2. Elements of Chemical Reaction Engineering - H. Scott Fogle
3. Biochemical Engineering Fundamentals - James E. Bailey, David F. Ollis
References:
1. Bioprocess Engineering Principles - Pauline M.Doran
2. Bioprocess Engineering Basic Concepts - Michael L. Shuler, FikretKarg.
COURSE PLAN
COURSE NO: 07BT62104
COURSE TITLE: BIOREACTOR DESIGN
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE: 1 Introduction and Review of Bio-reaction

engineering concepts, Mass transfer effects in heterogeneous
reaction, Continuous stirred tank and plug flow reactor performance 8 15
equations. Modeling of non-ideal behavior in bio reactors- problem
and solution. Tanks in series Model. Dispersion Models with
chemical reaction. Applications to design of continuous sterilizers –
problems and solution.

MODULE : 2Continuous Stirred Tank Bioreactor : performance

equation for M-M kinetics, substrate inhibition kinetics and product
inhibition kinetics, chemostat with cell cultures –steady state cell 6 15
and substrate concentrations and productivity as a function of
dilution rate, CSTR with immobilized enzymes, operation of CSTR
in a constant feed rate policy

FIRST INTERNAL TEST

MODULE : 3 Fed–batch reactor: Applications of fed reactor, Fed

batch operation of mixed reactor, material balance on cell and
substrate Recycle system: Chemostat with recycle, Biological waste 6 15
water treatment, Feed forward control of the activated sludge
process

MODULE : 4 The Transient Behavior of Bioreactors: Stability

analysis, Stability of the chemostat, Stability of chemostat with
substrate inhibition, Operating diagram, Transient responses of the 6 15
chemostat, control of the chemostat, Instrumentation and control of
bioreactor: Methods of measuring process variables, measurement
and control of dissolved oxygen, pH measurements

61
 SECOND INTERNAL TEST

MODULE : 5 Design of a fermenter: Basic function of a

fermenter for microbial or animal cell culture, basic bioreactor
design criteria, overview of bioreactor types-stirred tank bioreactor,
bubble column bioreactor, air-lift reactor, propeller loop reactor, jet 10 20
loop reactor, schematic overview of a fermenter with control system,
operating issues that affect reactor design, aeration and oxygen mass
transfer in bioreactor system, design of chemostat

MODULE: 6 Introduction to structured and unstructured

Models for bioprocess systems. Structured compartment 6 20
Model – Williams and Ramakrishna Model ,single cell Model,
metabolic Model, Genetically structured Model, Model
simulation using MATLAB, Simulink.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62106

Course Title:GENOMICS& PROTEOMICS
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Understanding structural, functional and comparative genomics its application for
health applications and drug discovery

Course Content/ Syllabus:

Human Genome Project-cloning-vectors-DNA batabanks-coding sequences-Protein sequence

information-protein databanks-Tools and techniques in proteomics-3-dimensional structure
determination-.Phylogenetic analysis.

62
Course Outcome:
1. Use the different methodologies, techniques and tools commonly used in proteomics
and genomics.

Text Books:

1. Bioinformatics; Methods and applications; Genomics, Proteomics and Drug

Discovery; (Rastogi, S. C. and Mendiratta and Rastogi, P)
2. Bioinformatics; A practical guide to the analysis of genes and proteins.; Edited by,
Andreas D. Baxevanis and Francis Oulelette

References: _
1. Gibson G & Muse SV. 2004. A Primer of Genome Science. Sinauer Associates.
2. Primrose SB &Twyman RM. 2007. Principles of Genome Analysis and Genomics.
Blackwell.
3. Sensen CW. 2005. Handbook of Genome Research. Vols. I, II. Wiley-CVH.
COURSE PLAN
COURSE NO: 07BT62106
COURSE TITLE: GENOMICS & PROTEOMICS
(L-T-P :3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE: 1Goals of the Human Genome Project, cloning
vectors, concept of maps, physical maps, shotgun libraries, DNA
polymorphism, nucleotides, DNA sequences. Sequence 6 15
databases: Gene Bank, EMBL Nucleotide sequence databank,
DNA Data Bank of Japan (DDBJ), database formats

MODULE:2Recombinant DNA technology, restriction

enzymes, resource for restriction enzyme (REBASE), similarity
search. Polymerase chain reaction, primer selection for PCR, 6 15
BLASTn, application of Bio Edit.Genome information and
special features, coding sequences (CDS), un-translated regions
(UTR’s),

63
 FIRST INTERNAL TEST

MODULE: 3cDNA library, expressed sequence tags (EST).

Approach to gene identification; masking repetitive DNA,
database search, codon-bias detection, detecting functional sites 6 15
in the DNA. Internet resources for gene identification, detection
of functional sites, gene expression. Introduction, Basic steps for
gene expression

MODULE: 4Concept of microarrays; spotted arrays,

oligonucleotide arrays, designing the experiment, Two-color
microarray experiments. Protein sequence information,
composition and properties, physic chemical properties based on 8 15
sequence, sequence comparison, Primary and Secondary
databases. Pair-wise sequence alignment, gaps, gap-penalties,
scoring matrices, PAM250,BLOSUM62, local and global
sequence alignment, multiple sequence alignment, useful
programs, ClustalW, BLASTp

SECOND INTERNAL TEST

MODULE : 5Proteomics classification; Tools and techniques in

proteomics; 2-D gelelectrophoresis, gel filtration, PAGE,
isoelectric focusing, affinity chromatography, HPLC, ICAT, 8 20
fixing and spot visualization, Mass spectroscopy for protein
analysis, MALDI-TOF,

MODULE: 6Electrospray ionization (EST), Tandem mass

spectroscopy (MS/MS)analysis; tryptic digestion and peptide
fingerprinting (PMF), Protein Micro array inproteinexpression, 8 20
profiling and diagnostics, drug target discovery. Database
searching, 3-dimensional structure determination by X-ray and
NMR. Phylogenetic analysis

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT62108

Course Title:BIOFUEL ENGINEERING
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil

64
Course Objectives:
 To understand the concepts, systems, and technology now being used to produce
biofuels on both an industrial and small scale

Course Content/ Syllabus:

Biofuels-Biofuel Production-Production of Biohydrogen-Production of Bioethanol-
Production of Biodiesel-Microbial Fuel Cells-Design and Treatment Effectiveness-Microbial
Modeling of Biofuel Production.

Course Outcome:
1. Develop experimental plan and conduct lab works pertinent to biofuel production.

Text Books:
1. Caye M. Drapcho, N.P. Nhuan and T. H. Walker, Biofuels Engineering Process
Technology , McGraw Hill Publishers, New York, 2008.
2. Jonathan R.M, Biofuels – Methods and Protocols (Methods in Molecular Biology
Series), Humana Press, New York, 2009.

References:
1. Lisbeth Olsson (Ed.), Biofuels (Advances in Biochemical
Engineering/Biotechnology Series, Springer-Verlag Publishers, Berlin, 2007

COURSE PLAN
COURSE NO: 07BT62108
COURSE TITLE: BIOFUEL ENGINEERING
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE : 1Introduction:Description of Biofuels; Energy
Use & Efficiency; Biofuel Production; Alternative Energies;
Biochemical Pathways Review for Organoheterotrophic, 6 15
Lithotrophic& Phototrophic Metabolism; Importance of
COD; Biofuel Feedstocks: Starch, Sugar, Lignocellulosic,
Agro & Industrial by-products.

MODULE : 2 Production of Biohydrogen:Enzymes

65
involved in H2 Production; Photobiological H2 Production:
Biophotolysis and Photofermentation; H2 Production by 6 15
Fermentation: Biochemical Pathway, Batch Fermentation,
Factors affecting H2 production, Carbon sources, Process
and Culture Parameters; Detection and Quantification of H2

FIRST INTERNAL TEST

MODULE : 3 Production of Bioethanol:Process Technology

for Bioethanol production using Sugar; Starch and
LignocellulosicFeedstocks: Selection of micro-organisms 6 15
and feedstock; Associated Unit Operations; Determination of
Bioethanol yield; Recovery of Bioethanol; Recent Advances;
Process Integration

MODULE : 4 Production of Biodiesel: Chemical,

Thermodynamic & Reaction Kinetic Aspects of Biodiesel
Production: Transesterification and Supercritical 6 15
Esterification, Saponification and Hydrolysis, Acid & Base
Catalysis; Sources of Oils; Methods of Biodiesel Production
– General procedure and Large scale production; Quality
Control Aspects.

SECOND INTERNAL TEST

MODULE : 5 Microbial Fuel Cells:Biochemical Basis; Fuel

Cell Design: Anode & Cathode Compartment, Microbial
Cultures, Redox Mediators, Exchange Membrane, Power
Density; MFC Performance Methods: Substrate & Biomass 10 20
Measurements, Basic Power Calculations, MFC
Performance: Power Density, Single-Chamber vs Two-
Chamber Designs, Wastewater Treatment Effectiveness;
Future Directions

MODULE : 6 Microbial Modeling of Biofuel Production:

Microbial Growth Moduleels: Unstructured, Single Limiting 8 20
Nutrient Models, Inhibition Models, Models for Multiple
Limiting Substrates, Yield Parameters; Kinetic Rate
Expressions; Bioreactor Operation and Design for Biofuel
Production: Batch, CSTR, CSTR with Cell Recycle, Fed-
Batch Systems, Plug Flow Systems; Modeling of Glucose
Utilization and Hydrogen Production; Batch and CSTR
Fermentations and Simulations .

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial

66
 End Semester Examination 60

ELECTIVE IV
07BT7201X

67
Course No: 07BT72011
Course Title:ADVANCED BIOSEPARATION TECHNIQUES
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Understand the principles and theory of Chromatography, mechanism of interaction
and its applications in separation and analysis biomolecules such as proteins,
peptides and small molecules of therapeutic importance.

Course Content/ Syllabus:

Role of Downstream Processing in Biotechnology: Role and importance of downstream

processing in biotechnological processes. Economics and downstream processing in
Biotechnology. Physicochemical basis of bio-separation processes. Precipitation & Extraction
methods: Precipitation with salts, organic solvents &polymers. Membrane-based separations
(micro- & ultra-filtration) Principles of chromatographic separation Case studies: Preparation
of commercial enzymes

Course Outcome:
1. To understand the importance of downstream processing in Biotechnology
2. To familiarise with precipitation and extraction methods

References
1. Belter P.A, Cussler E and Wei Shan Hu, Bioseparation – Downstream Processing for
Biotechnology, Wiley Interscience, 1988.

68
 2. Asenjo and Juan A. Asenjo, Separation Processes in Biotechnology, CRC Press,
1990.
3. Wankat P.C, Rate Controlled Separation, Kluwer Publishers, 1990.
4. Wang D.I.C, Cooney C.L, Demain A.L, Dunnil.P, Humphery A.E. and Lilly M.D.
Fermentation and Enzyme Technology, John Wiley and Sons, 1979.

COURSE PLAN
COURSE NO: 07BT72011
COURSE TITLE: ADVANCED BIOSEPARATION TECHNIQUES
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE: 1 Role of Downstream Processing in Biotechnology: Role
and importance of downstream processing in biotechnological
processes. Problems and requirements of bio-product-purification.
Economics and downstream processing in Biotechnology. Cost cutting 10 15
strategies, characteristics of biological mixtures, process design criteria
for various classes of bio-products (high volume-low value products and
low volume- high value products),

MODULE: 2 Physicochemical basis of bio-separation processes.

Primary Separation and Recovery Processes: Cell disruption methods
for intracellular products, removal of insolubles, biomass (and 8 15
particulate debris) separation techniques; flocculation and
sedimentation, centrifugation and filtration methods

FIRST INTERNAL TEST

MODULE: 3Precipitation & Extraction methods: Precipitation with

salts, organic solvents &polymers. Batch extractions, staged extractions-
cross current, co current, counter current extractions. Differential 8 15
extractions, fractional extractions with a stationary phase, fractional
extractions with two moving phases. Reverse micelle extraction,
supercritical fluid extraction, in-situ product removal/integrated
bioprocessing.

MODULE : 4 Membrane-based separations (micro- & ultra-filtration):

Theory; design & configuration of membrane separation equipment;

69
applications; reverse osmosis, dialysis, electro dialysis, Iso-electric 6 15
focusing.

SECOND INTERNAL TEST

MODULE : 5Adsorption: Adsorption isotherms, industrial adsorbents,

adsorption equipments for batch and continuous operations (co current
and counter current), adsorption in fixed beds. 4 20

MODULE : 6
Chromatography: Principles of chromatographic separation – gel 8 20
filtration, reversed phase, hydrophobic interaction, ion-exchange,
expanded bed adsorption, bio affinity and IMAC, supercritical fluid
chromatography.
Case studies: Preparation of commercial enzymes: Continuous
isolation of enzyme prolyl-t RNA synthetase from mung bean,
Intracellular foreign proteins from recombinant E.coli and extracellular
enzyme (protease) recovery; Purification of biosurfactants from
fermentation broths.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT72013

Course Title:INTELLECTUAL PROPERTY RIGHTS (IPR) FOR A GLOBAL BIO-
ECONOMY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 An understanding of IPR and Patent Systems in India. Development of a global
standardization of patenting and the emerging of economies based on IPR.
Course Content/ Syllabus:

70
Introduction to Patent and other IPRs, Types of IP, Patentability requirements-inventive-step,
industrial applicability and disclosure requirements Biotechnological Invention by
documentation and Search, Precautions while patenting disclosure/non-disclosure, Drafting
of Patent in field of Biotechnology Bioprospecting&BiopiracyBiopiracy case studies ,
TRIPS- Does it favour Global transfer of Biotechnology Agriculture and food security:
Intellectual property rights and the trade in seeds; Genetic Use Restriction Technologies

Course Outcome:

1. To better understand about the IPR and patenting systems in the field of
Biotechnology
References:
1. Kankanala. C., Genetic Patent Law & Strategy, 1stEdition,
2. BAREACT, Indian Patent Act 1970, Acts & Rules
3. Laurence Liang, ‘Beyond Representation. The Figure of the Pirate’. Available
at:http://www.altlawforum.org/PUBLICATIONS/Beyond%20Representation.doc
4. Vandana Shiva, Bioprospecting as Sophisticated Biopiracy (2007) Signs 32(2):
pp :307-313.
5. Convention on Biological Diversity, at: http://www.biodiv.org/convention/articles.asp
6. UNEP/CBD/WG-ABS/2/3, 2003, `The Role of Intellectual Property Rights in Access and
Benefit-Sharing Arrangements’ at: http://www.biodiv.org/doc/meetings/abs/abswg-
02/official/abswg-02-03-en.pdf
7. Carlos Correa, Traditional Knowledge and Intellectual Property: Issues and Options
Surrounding Protection of Traditional Knowledge (2001), available at:
http://www.geneva.quno.info/pdf/tkmono1.pdf
8. Cori Hayden, When Nature Goes Public. The Making and Unmaking of Bioprospecting in
Mexico (Princeton: PrincetonUniversity Press, 2003.
9. Drahos ‘Negotiating Intellectual Property Rights. Between Coercion and Dialogue’ in
Drahos&Mayne (eds), Global Intellectual Property Rights (2002) 161-182.
10. Graham Dutfield, Intellectual Property Rights and the Life Science Industries. A Twentieth-
Century History (2003), chapter 8.
11. Glen Burgos & Dan Kevles, Plants as Intellectual Property: American Practice, law, and
policy in World Context (1992) 7 Osiris 74-104.
12. Michael Blakeney, ‘Stimulating Agricultural Innovation’, in Maskus& Reichmann,
International Public Goods and Transfer of Technology Under a Globalized Intellectual
Property Regime, chapter 14.

71
 13. BiswajitDhar, Sui generis systems for plant variety protection, available
at:http://www.geneva.quno.info/pdf/sgcol1.pdf
14. PrabuddhaGanguliIntellectualProperty Rights-Unleashing the Knowledge Economy. Tata
McGraw Hill Publishing Company Limited, New Delhi.
15. Beier, F.K, Crespi, R.S and Straus, T. Biotechnology and Patent protection –Oxfordand
IBH Publishing Co. New Delhi.
16. SassonA, Biotechnologies and Development, UNESCO Publications.

COURSE PLAN

COURSE NO: 07BT72013

COURSE TITLE: INTELLECTUAL PROPERTY RIGHTS (IPR) FOR A GLOBAL BIO-
ECONOMY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE : 1Introduction to Patent and other IPRs, Types of IP:
Patents, Trademarks, Copyright & Related Rights, Industrial Design,
Traditional Knowledge, Geographical Indications. Patent protection, 8 15
Patentability requirements-inventive-step, industrial applicability and
disclosure requirements.

MODULE : 2Biotechnological Invention by documentation and

Search, Drafting of Patent in field of Biotechnology, Patent filing in 15
India and in abroad, Successful research and commercialization of 6
biotechnological inventions

FIRST INTERNAL TEST

MODULE : 3Precautions while patenting disclosure/non-disclosure,

Patent infringement- meaning, scope, litigation. Diamond VS
Chakrabarty Case. Orphan Drugs and diseases, The 90/10 research gap, 6 15
Effect of TRIPS on drug prices in the developing world.

MODULE : 4The International Debate on Traditional Knowledge as

Prior Art in the Patent System’, Bioprospecting&Biopiracy, Biopiracy
case studies (Case studies of Neem /Turmeric/Arogyapacha of
KaniTribals in Kerala/Rosy Periwinkle of Madagascar)-Traditional 8 15
Knowledge Digital Library- Need and Development.. CBD as a treaty
for protecting Traditional Knowledge.

SECOND INTERNAL TEST

72
MODULE: 5TRIPS- Does it favour Global transfer of Biotechnology?
CBD and the need of CBD for global developing countries. Regime
shifting between TRIPS and Other agreements in Global lawmaking.’ 8 20
Industry Strategies for Intellectual Property and Trade: The Quest for
TRIPS and Post-TRIPS Strategies’

MODULE: 6Agriculture and food security: Intellectual property rights

and the trade in seeds; Genetic Use Restriction Technologies-
Terminator and Traitor technology. UPOV Treaty- disadvantages for 6 20
India, Indian PPVFR Act 2001.Criteria for Plant variety (Novelty,
Distinct, Unique, Stable)

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT72015

Course Title:MOLECULAR MODELLING & DRUG DISCOVERY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
To understand the various molecular Modeling structures. To understand the basic principles
underlying drug design designing methods

 Course Content/ Syllabus:

Modeling basics. Generation of 3D Coordinates Crystal data, Fragment libraries Solvent
effects and Quantum Mechanical methods Conformational analysis Computational tools for
Molecular Modeling, conformational analysis using - Systematic Search Procedures, Monte
carlo and molecular dynamics methods Force field, quantum chemistry, Schrödinger
equation, potential energy functions, energy minimization Analog based drug design, Drug
discovery area, pharmaco genetics and pharmacogenomics applications, computer-aided
drugdesigning methods.

Course Outcome:

73
 1. To familiarise with molecular modelling and simulation principles and softwares
COURSE NO: 07BT72015
COURSE TITLE: MOLECULAR MODELLING & DRUG DISCOVERY
(L-T-P : 3-0-0) CREDITS:3

References:
1. Principles and applications of Modeling by Leach
2. Molecular Modeling by the Hans Peter Heltie&GerdFalkens, VCH.
3. Chemical application of molecular modeling,Jonathan Goodman.
4. Computational chemistry by Guy H Grant & W.Graham,Oxford University

74
 MODULES Contact Sem.Exam
hours Marks;%

MODULE : 1Modeling basics. Generation of 3D Coordinates Crystal

data, Fragment libraries, and conversion of 2D Structural data into 3D 6 15
Form, Force fields, and Geometry optimization, Energy minimizing
Procedures, use of Charges, Solvent effects and Quantum Mechanical
methods. Conformational analysis.

MODULE: 2Computational tools for Molecular Modeling,

conformational analysis using - Systematic Search Procedures, Monte
carlo and molecular dynamics methods. Determining features of proteins 8 15
(Interaction potential, Molecular Electrostatic Potential, molecular
interaction fields, properties on molecular surface and Pharmacophore
identification. 3DQSAR Methods.
FIRST INTERNAL TEST

MODULE : 3Introduction, force field, quantum chemistry, Schrödinger

equation, potential energy functions, energy minimization, local and
global minima, saddle point, grid search, various approximations; LCAO, 8 15
HF, semi-empirical calculations; single point calculations, full-geometry
optimization methods, ZDO, MNDO, CNDO, NDDO, AM1,PM3, RM1,
conformational search, Z-matrix, docking, molecular Modeling packages.

MODULE : 4Comparative protein modeling: Modeling by Homology

the alignment, construction of frame 6 15
Work, selecting variable regions, side chain placement and refinement,
validation of protein
Models – Ramchandran plot, threading and ab initio modeling.
SECOND INTERNAL TEST

MODULE: 5Analog based drug design: Introduction to QSAR, lead 6

Module linear and nonlinear Modeled equations, biological activities, 20
physicochemical parameter and molecular descriptions, molecular
Modeling in drug discovery.

MODULE : 6Introduction, drug discovery area, pharmaco genetics and

pharmacogenomics applications, SNPs, parameters in drug discovery
identification of drug target molecules, drug design and its approaches, 8 20
computer-aided drug designing methods; computer aided molecular
design (CAMD), Quantum CAChe and project leader, ligand design
methods, docking programs; De novo design

END SEMESTER EXAMINATION

COURSE PLAN
ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

75
 Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT72017

Course Title:FUNDAMENTALS OF SYNTHETIC BIOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Design and construction of new biological functions and systems not found in nature.

Course Content/ Syllabus:

Introduction to Biotechnology and Synthetic Biology- History, Goals, Applications, and
Methods Overview The Central Dogma of Biology.DNA Structure & Modification Processes
Synthetic Biology: Design Paradigm (Parts, Devices, Systems) The importance of network
structure in cellular networks .Review of continuous and stochastic Models of cellular
networks Control systems in metabolism. Control systems in protein networks Metabolic
engineering strategies. Protein networks, control and dynamical analysis

Course Outcome:
1. To acquire knowledge about the basic concepts in synthetic biology, network
structures in cellular level and control systems in metabolism.

References:
1. Synthetic biology-Industrial and environmental applications by Markus Schmidt
2. System biology and synthetic biology by Pengcheng Fu &svenpanke.
3. The emergence of life- from chemical origins to synthetic biology By Pier Luigi
Luisi.

COURSE PLAN

76
 COURSE NO: 07BT72017
COURSE TITLE: FUNDAMENTALS OF SYNTHETIC BIOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE :1Introduction to Biotechnology and Synthetic Biology-
History, Goals, Applications, and Methods Overview, Risks of
engineered systems, Synthetic Biology Standards, ethics of the field 8 15

MODULE : 2The Central Dogma of Biology.DNA Structure &

Modification Processes. PCR Technology, DNA Sequencing & 8 15
Synthesis Technology. Different application areas of engineered
bacteria

FIRST INTERNAL TEST

MODULE: 3Synthetic Biology: Design Paradigm (Parts, Devices,

Systems).Cloning. Cloning vs. Bio-Brick Assembly Process. 6 15
Fundamental Engineering Concepts in Application to Synthetic
Biology.

MODULE: 4The importance of network structure in cellular networks

.Review of continuous and stochastic Models of cellular networks. The 8 15
interplay between structure and dynamics. Bifurcation analysis and
evolutionary design approaches in synthetic biology. Standards and
ontologies (SBML, CellML, PoBoL, CAD in synthetic biology).

SECOND INTERNAL TEST

MODULE: 5Control systems in metabolism. Control systems in

protein networks. Robustness and small signal analysis of cellular 6 20
pathways. Advanced structural analysis including elementary Models,
FBA and MFA.

MODULE: 6Metabolic engineering strategies. Protein networks,

control and dynamical analysis. Protein network engineering. 6 20

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

77
 Maximum marks :100
Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

78
ELECTIVE V
07BT7203X

79
Course No: 07BT72031
Course Title:TISSUE ENGINEERING & BIOMATERIALS
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 To understand the basics to synthesize materials that can stimulate beneficial
biological responses from the body and use them for tissue repair

Course Content/ Syllabus:

Structure of solids. Review of basic concepts Biomaterials, definition, classification Structure

property relation. Characterization of biomaterials.Tissue environment of the implanted
biomaterial: unit cell processes Survey of clinical cases of biomaterials-tissue interactions,
Tissue structures and unit cell processes Cell-surface interactions: Analysis of surfaces of
biomaterials and protein adsorption, In vivo and clinical case studies: Blood and tissue
compatibility of biomaterials and their in vitro and in vivo assessment In vivo synthesis of
skin, In vivo synthesis of peripheral nerve, Rules for synthesis of tissues and organs, Joints
and dental tissues

Course Outcome:
1. To understand the applications of Tissue Engineering and basic concepts involved
2. To familiarise with various biomaterials used in tissue engineering.

References
1. Ratner, Hoffman, Schoen Biomaterial science- an introduction to materials in
medicineAcademic press
2. Bernhard Palsson, Sangeeta Bhatia ,Tissue Engineering, Pearson Prentice Hall, 2003
3. Robert. P.Lanza, Robert Langer & William L. Chick, Principles of tissue engineering,
Academic press,1997
4. Park .J.B. Biomaterials- science and engineering, Plenum press
5. Sharma C.P., Szycher.MBlood compatible materials and devices Technomic
publishing company
6. R. M. Johnson, R. M. Mwaikambo, Tucker Biopolymers Rapra technology.

80
 COURSE PLAN
COURSE NO: 07BT72031
COURSE TITLE: TISSUE ENGINEERING & BIOMATERIALS
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE : 1 Structure of solids. Review of basic concepts.

Biomaterials, definition, classification. Polymers, metals, alloys,
ceramics and composites, physical, chemical and mechanical 8 15
aspects of bulk and surface properties of metallic ,polymer and
ceramic biomaterials (in vivo and in vitro ) Corrosion studies.

MODULE : 2 Structure property relation. Characterization of

biomaterials. Bulk analysis-XRD, FTIR, SEM, TGA etc. Surface 6 15
analysis-XPS, SIMS, AES, STM etc

FIRST INTERNAL TEST

MODULE : 3 Tissue environment of the implanted biomaterial:

unit cell processes Survey of clinical cases of biomaterials-tissue
interactions, Tissue structures and unit cell processes, Integrins 15
8
and adhesion proteins
Unit cell processes comprising the healing response, Unit cell
processes underlying tissue engineering, Structure and function of
naturally occurring ECMs and its regeneration

MODULE : 4 Cell-surface interactions: Analysis of surfaces of

biomaterials and protein adsorption, Phenotype changes following
adhesion on biomaterials, Structural determinants of biologically
15
active materials, Methodology for cell-surface interactions, Cell-
8
scaffold interactions during regeneration, Non-cooperative cell-
surface interactions, From randomness to co-operativity. Scaffold
and transplant- Engineering biomaterials, Degradable materials,
porosity, mechanical strength, 3-D architecture and cell
incorporation.

SECOND INTERNAL TEST

MODULE: 5 In vivo and clinical case studies: Blood and tissue

compatibility of biomaterials and their in vitro and in vivo
assessment. Tissue response to implants; biocompatibility, 6 20
Epithelialization (epidermal regeneration) and endothelialization
of vascular prostheses,

MODULE: 6 In vivo synthesis of skin, In vivo synthesis of

peripheral nerve, Rules for synthesis of tissues and organs, Joints 6 20

81
and dental tissues: prosthetic replacement, Implants for bone
regeneration, Regeneration of soft musculoskeletal tissues,
Biomaterial applications in the heart and other organs. Ethical,
FDA and regulatory issues.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT72033

Course Title:NANOBIOTECHNOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Provide basic knowledge in the interface between chemistry, physics and biology on
the nano structural level with a focus on biotechnological usage

Course Content/ Syllabus:

The Science of Nano - What is Nanobiotechnology, Cellular nanostructures Introduction to
Nanostructures : Carbon Nanotubes (CNT), Fullerenes (C60, C300) Nano Peapods, Quantum
Dots and Semiconductor Nanoparticles Metal-based Nanostructures (Iron Oxide
Nanoparticles) Protein-based Nanostructures: Nanomotors: Bacterial (E.coli) and Mammalian
(Myosin family). Nanobiosensors: Science of Self-assembly - From Natural to Artificial
Structures Nanotechnology & Microfluidics Nanoparticles: Synthesis and Applications.
Applications of Nanostructures in Drug: Discovery, Delivery, and Controlled Release
Nanotechnology for Tissue Engineering: Applications in Regenerative Therapy

Course Outcome:
1. To understand the basic concepts in nanotechnology

82
 2. To understand the significance of nanotechnology and its applications in the field of
biotechnology

References:
1. GeroDecher, Joseph B. Schlenoff, Multilayer Thin Films, Wiley-VCH Verlag GmbH
& Co. KGaA, 2003
2. David S. Goodsell, Bionanotechnology : Lessons from Nature, Wiley-Liss , 2004.
3. Kenneth J. Klabunde , Nanoscale Materials in Chemistry , John Wiley & Sons, Inc.,
2004
4. Nanobiotechnology: Concepts, Applications and Perspectives by Christof M.
Niemeyer and Chad A. Mirkin Wiley-VCH; 1 edition, 2004

COURSE PLAN
COURSE NO: 07BT72033
COURSE TITLE: NANOBIOTECHNOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE : 1The Science of Nano - What is Nanobiotechnology,
Cellular nanostructures, self-assembly of colloidal nanostructures of 6 15
biological relevance, bioactive nanoparticles (respiratory surfactants,
magnetic nanoparticles),

MODULE : 2Introduction to Nanostructures : Carbon Nanotubes

(CNT), Fullerenes (C60, C300) Nano Peapods, Quantum Dots and 8 15
Semiconductor Nanoparticles Metal-based Nanostructures (Iron Oxide
Nanoparticles), Nanowires Polymer-based Nanostructures
(Dendrimers), Gold Nanostructures: (Nanorods, Nanocages,
Nanoshells)

FIRST INTERNAL TEST

MODULE : 3Protein-based Nanostructures: Nanomotors: Bacterial

(E.coli) and Mammalian (Myosin family). Nanobiosensors: Science of
Self-assembly - From Natural to Artificial Structures. Nanoparticles in 6 15
Biological Labeling and Cellular Imaging: Science of Nanoparticles
Functionalization

MODULE : 4Nanotechnology & Microfluidics: Nano Printing of

DNA, RNA, and Proteins Biochips Applications in Nano Scale 8 15
Detection, Lab-on-a-chip Devices (LOC), Medical Applications of
Nanobiotechnology: Nanoparticles’ Cytotoxicity.

83
 SECOND INTERNAL TEST

MODULE : 5Nanoparticles: Synthesis and Applications. Applications

of Nanostructures in Drug: Discovery, Delivery, and Controlled 20
Release. Nanostructures in Cancer Research: Examples of 8
Nanostructures in Research and Therapy.

MODULE : 6Nanotechnology for Tissue Engineering: Applications in

Regenerative Therapy. Nanotechnology in Tissue Engineering, 6 20
Microemulsions and Drug Delivery in Nanotechnology.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

Course No: 07BT72035

Course Title:MANAGEMENT ENTREPRENEURSHIP & BIO-BUSINESS
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil
Course Objectives:
 Impart technical knowledge and develop managerial skills for managing Bio -
Technology business
 To promote entrepreneurship amongst students to venture in Bio - Technology
business

Course Content/ Syllabus:

Management: Introduction: Meaning nature and characteristics of Management, scope and
functional areas of management Managing Engineering Design and Development: Product
and Technology Life Cycles, Nature of Research and development, Research Strategy and
organization Managing Production Operations Entrepreneur: Meaning of an Entrepreneur,
Role of entrepreneurs in Economic Development, Entrepreneurship in India,
Entrepreneurship Barriers Preparation of Project:Meaning of Project, Project Identification,

84
Project Selection, Project Report –Contents, Formulation and Project Appraisal Technologies
and Bio-Safety: Principles of business management and concept of Bio-business
Commercialization: Analysis of factor influencing international competitiveness in
biotechnology Project Cost and Market Potential: Total product cost, capital investment and
profitability

Course Outcome:
1. To inculcate financial management and business skills.

References:
1. R . A . B a r o n , S . A . S h a n e ; E n t r e p r e n e u r s h i p , T h o m s o n , 2 0 0 4 , ISBN 0-
324-27356
2. Small Business Management: Entrepreneurship and Beyond, Timothy S Hatten

COURSE PLAN
COURSE NO: 07BT72035
COURSE TITLE: MANAGEMENT ENTREPRENEURSHIP & BIO-BUSINESS
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%
MODULE : 1 Management: Introduction: Meaning nature and
characteristics of Management, scope and functional areas of
management, Management and administration, roles of management, 8 15
levels of management. Development of Management Thought: Early
management approaches and Modern management approaches.
Functions of Management: Planning and Forecasting, Organizing,
Directing and Controlling.

MODULE : 2 Managing Engineering Design and Development:

Product and Technology Life Cycles, Nature of Research and
development, Research Strategy and organization, selecting R & D
Projects, Protection of Ideas. Creativity, Nature of Engineering Design,
Systems Engineering / New Product Development, Control System in
6 15
Design, Product Liability and Safety, Designing for Reliability, other
“abilities” in Design.
Managing Production Operations: Assuring product quality,
Productivity, Work measurement, Maintenance and Facilities (Plant)

85
engineering and other manufacturing functions.

FIRST INTERNAL TEST

MODULE : 3 Entrepreneur: Meaning of an Entrepreneur, Role of

entrepreneurs in Economic Development, Entrepreneurship in India,
Entrepreneurship Barriers. Role of Micro Small & Medium Enterprises
(MSME) in Economic Development, Impact of Liberalization, 6 15
Privatization and Globalization on MSME, Effect of WTO/GATT.
Different Schemes; TECSOK, KIADB, KSSIDC, KSIMC, DIC-Single
Window Agency, MSME, NSIC, SIDBI, KSFC.

MODULE : 4 Preparation of Project: Meaning of Project, Project

Identification, Project Selection, Project Report –Contents, Formulation
and Project Appraisal. Identification of Business Opportunities: Market 8 15
Feasibility studies, Technical Feasibility Studies; Financial Feasibility
Studies and Social Feasibility studies.

SECOND INTERNAL TEST

MODULE : 5 Technologies and Bio-Safety: Principles of business

management and concept of Bio-business, Fundamentals and 8 20
constituents of Biotech for bio-business, SWOT analysis of Indian Bio-
business.
Commercialization: Analysis of factor influencing international
competitiveness in biotechnology, type of firms commercializing
biotechnology, financing, tax incentives, issues and policies. Bioscience
enterprises- raw bio-commodities, hybridization, tissue culture, bio-
fermentation, bio-fertilizers.

MODULE : 6 Project Cost and Market Potential: Total product cost,

capital investment and profitability, manufacturing cost estimation,
capital investment estimation, Risk capital and working capital, 6 20
manufacturing cost estimation for an intracellular protein, using cost
analysis for R & D decision making.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Exam 2 15
Internal Assessment
10
Assignment/Mini Project/Tutorial
End Semester Examination 60

86
Course No: 07BT72037
Course Title:STRUCTURAL BIOLOGY
Credits: 3-0-0:3
Year: 2015
Pre-requisites: Nil

Course Objectives:
 Study protein and nucleic acid structure and function, focusing on energetic forces
that guide folding, and computer Modeling to predict structures

Syllabus:
Nucleic acid structures, RNA folding, RNA loops, conformational study, various ribose ring
conformations Over expression of recombinant protein, Construction of an over expression
system In vivo expression system:Escherichia coli, yeast (S. cerevisiae, P. pastries etc.),
insect cells. In vitro expression system-cell-free system. Protein purification Crystallization:
Crystallization techniques Protein Structure Prediction; Homology Modeling, prediction of
protein structure from sequences Predicting trans-membrane helices

Course Outcome:
1. To understand the biomolecules in structural and functional level
2. To understand the basic principles of modelling.

References:
1. Introduction to Protein Architecture, by A.M.Leak
2. Introduction to Protein Structure, by Banden and Tooze.
3. Tinoco, Ignacio, Jr., Sauer, Kenneth, Wang, James C., & Puglisi, Joseph D. (2001)
Physical Chemistry: Principles and Applications in Biological Sciences, 4th ed.
Prentice Hall, ISBN: 0-13-095943-X
4. vanHolde, Kensal E., Johnson, W. Curtis, & Ho, PuiShing (1998) Principles of
Physical Biochemistry. Prentice Hall. ISBN 0-13-720459-0
5. Cantor, Charles, and Schimmel, Paul (1980) Biophysical Chemistry, Vols. I-III, W.
H. Freeman and company, San Francisc

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 COURSE PLAN
COURSE NO: 07BT72037
COURSE TITLE: STRUCTURAL BIOLOGY
(L-T-P : 3-0-0) CREDITS:3

MODULES Contact Sem.Exam

Hours Marks;%

MODULE : 1 Nucleic acid structures, RNA folding, RNA loops,

conformational study, various ribose ring conformations, ribose-ring
puckering, protein-protein interactions, protein ligand interactions, 8 15
DNA-binding proteins, RNA-binding proteins, Ramachandran plot,
,3-dimensional structures of membrane proteins, importance of 310
helix and loops, biophysical aspects of proteins and nucleic acids.

MODULE : 2 Over expression of recombinant protein,

Construction of an over expression system : Cloning the gene 6 15
encoding the target protein in cloning vector, Over expression of the
protein as a recombinant protein using expression vector in
appropriate host cells.

FIRST INTERNAL TEST

MODULE : 3 In vivo expression system:Escherichia coli, yeast (S.

cerevisiae, P. pastries etc.), insect cells. In vitro expression system-
cell-free system. Protein purification: Sonication, Chromatography, 8 15
Affinity chromatography, Gel filtration, Hydrophobic interaction
chromatography, Ion exchange chromatography, Confirmation of
the purity of the protein,

MODULE : 4 Crystallization: Crystallization techniques: Sitting

drop vapor diffusion, Hanging drop vapor diffusion, Micro batch 8 15
under oil, Dialysis. Data collection - X-ray diffraction data. Phase
determination and calculation of electron density, Modeling and
Structure Refinement,

SECOND INTERNAL TEST

MODULE : 5 Protein Structure Prediction; Homology Modeling,

prediction of protein structure from sequences, functional sites,
Protein folding problem, protein folding classes, protein 7 20
identification and characterization; AACompIdent, TagIdent,
PepIdent and MultiIdent; PROSEARCH, PepSea, Pep MAPPER,
Find Pept,

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MODULE : 6 Predicting trans-membrane helices, Primary structure
analysis and prediction, Secondary structure analysis and prediction, 5 20
motifs, profiles, patterns and fingerprints search. Methods of
sequence based protein prediction.

END SEMESTER EXAMINATION

ACADEMIC ASSESSMENT/EVALUATION

Maximum marks :100

Internal Exam 1 15
Internal Internal Exam 2 15
Assessment Assignment/Mini 10
Project/Tutorial
End Semester Examination 60

89