User Manual

Table of Contents

Page

1. General

1.1 Precautions Before Use.................................................................................5

1.2 Product Introduction.....................................................................................6

1.3 Product Configuration..................................................................................7

2. Installation

2.1 Product Installation.......................................................................................8

2.2 Product Specifications...................................................................................10

3. Product Usage

3.1 Product Usage.............................................................................................12

3.2 Names of Each Part......................................................................................15

3.3 Usage Instructions.......................................................................................18

4. Products Management Methods

4.1 Accessories.................................................................................................24

4.2 Maintenance and Management....................................................................25

4.3 Troubleshooting..........................................................................................26

5. Product Assurance

5.1 Quality Assurance........................................................................................27

 1
General
Precautions Before Use

This manual describes the performance and usage instructions of the product, and the
precautions in the handling. Please carefully read this manual, before using the
product. The following warnings should be strictly adhered to: (The following warning
sign is marked in the part that is required user attention, so please safely use the
product after being well-acquainted with the meaning.)

Warning
Failure to follow the instructions may result in injury.

Caution
Failure to follow the instructions may result in product failure.

Proximity Caution
Keep clear of the product during use.
Notification
Do not disassemble or modify the product or use it for purpose other than its
intended function.

Caution: High Temperature

High-temperature heating, pose a risk of burns.

Danger of Explosion
If volatile, explosive chemicals are used, there is a danger of explosion.

This symbol indicates Protective earth (ground).

This symbol indicates power “OFF” on the power on/off switch.

I This symbol indicates power “ON” on the power on/off switch.

This symbol indicates the connection point of the neutral conductor on the
N installed unit.

This symbol indicates alternating current Voltage.

1. General
1.2 Product introduction

The temperature control method of this product is Fuzzy control method, and the maximum
power is supplied nearby the set temperature, and it has been designed so that excellent
temperature safety is maintained, at the same time that the power supplied to the heater is
properly controlled and the temperature deviation is minimized after reaching to the setting
temperature. This product was developed and manufactured considering the best possible ease
for use and safety, and has the following features.

- Because Jog Dial Knob and Push Button which are rigid and have excellent operability are
mounted, this product has high operating sensitivity and excellent accuracy.
- This product is equipped with the emission steam condensation mechanism, so steam is
not generated from the sterilizer after the sterilization operation, and the safety and
convenience are provided to the user.
- The stainless steel material processed by a press is used in order to maintain the aesthetics
of Chamber and the high level of hermeticity, and the outside overheating of the equipment
is protected against in the maximum limit by using the advanced insulator.
- The sterilization environment optimized by the Liquid-/Solid-Cycle Mode
selectable
- The safety device for overheating prevention, and over current and short circuit prevention
circuitry that preferentially consider the user’s safety are mounted.
- When it reaches at the sterilization time, Buzzer is operated for 30 seconds and it gives the
convenience to the user.
- Because this product is equipped with the Safety Electronic Door Closing System which is
not opened for safety during operating, and the door is not opened at the temperature of
80℃ or more, and the safety for the user is provided.
- Two Wire Baskets suitable to each Model are supported.
- PL(Product Liability) Insurance
 1
Installation

*
1.1 Product Installation

Caution of Installation
2
2
1. Please be careful when transporting the product because it is heavy.
2. Please do not lay the product on the floor or subject it to shock. It can cause damage inside
the product.
3. Please install the product in a hard, flat, and stable place.
4. Please do not install the product in a place where it is exposed to direct sunlight, or in the
dangerous places.
5. To minimize the risk of short circuits, do not install the product (in the) places where it will
be subjected to the inflow of moisture or organic solvents on the regulator part or inside of
the body.
6. This product was manufactured for normal operation at a rated voltage, so please check the
voltage status before installing the product.
7. When installing and using the product, please certainly use the power cord provided with
the products.
* Installation Process

1. After receiving the unit and putting it on the specified place of use remove the wrapping.
2. After positioning the unit, lock the moving wheel in order to make position fixed and
the unit stable.
3. Check the unit whether there are eventual transport damages or not. In case there are
some transport damages found please inform the forwarder and your contractor. If there is
no damage, please go to next point 3.
4. Let the unit stand for a few minutes so that the all the internal parts and components of the
unit can be stabilized.
5. Open the door at the left side of the unit. You can find a room for pipe assembly and
electric distribution plane. Please check carefully if there is anything wrong including
disconnection of the electric wire, breakage of pipe or fitting element. In case there is any
kind of damage found, please inform the forwarder and your contractor. If you cannot find
anything wrong, please go to next point 5.
6. For normal use of the unit, confirm that the valve is open, and water drain valve is closed.
7. Connect the main power cord to the socket.
8. If such a big power supply line is not available in the room where the unit is installed, you
have to contact the appropriate person who is in charge of electric facilities in your
company or organization.
 * Safety Symbols on a product
- Safety symbols stickers Attached to side of product. For meaning of each symbol, please
refer to Precautions Before Use, Safety Instructions.

Safety symbols on units, for further

description of each symbol, please
refer to 3.1 Precautions for Use
Functional Block Diagram
 3
2. Product Usage
2.1 Precautions for Use

Be careful to operate the device.

You must read this manual carefully and completely before using.
The device should only be operated by laboratory personnel especially trained
for this purpose and familiar with all precautionary measures required for
working in a laboratory.
If unexpected sound, smell or smog is generated after connecting electric power,
pull out the main plug and contact manufacturer or a supplier.
Only demineralized or distilled water must be used. If dirty water is used, it will
cause damage of all the components inside the vessel, inner surface of the vessel,
pipes, fittings and valves.
Prior to sterilization, check sterilizing chamber to ensure there is sufficient feed
water. No sterilization without feed water!
Autoclaves have been designed for the following purposes;
 For sterilizing glassware, rubber items, pipette tips, instruments, filter bodies,
as well as consumables, waste in bags, and to destroy infectious or septic
materials.
 For sterilizing liquids, solutions and culture mediums in open or lightly closed
vessels.
 For sterilizing foodstuffs
 For streaming liquids or for dissolving agar.
 For non-pressurized disinfection of heat-sensitive culture media and solutions.
Liquids and foods must not be sterilized in pressure-sealed containers.
Easily combustible or toxic liquids must not be sterilized

Destruction bags must be open during sterilization, otherwise they may burst.

Before every sterilizing operation, check that the lid stiffener on the lid has moved
all the way into the lid clamp.
And check the lid screw handle is turned until it cannot be turned anymore.

Allow chamber pressure gauge to slow zero before opening the autoclave.

OPT sensor (SAFETY) knob should be set as operating temperature +20 ~ +30C.
Please be noted that the heater doesn’t work if the knob is set to 0 position.
Please avoid ultrasonic lamps.
The unit could be cracked or faded.
You must wear the following protective items when opening the sterilizing chamber
and removing the sterilized articles;
- Apron
- Eye protectors
- Heat insulated gloves
Watch for hot surfaces and hot steam!

After sterilizing liquids;

- Danger from retardation of boiling.
- For open vessels containing up to 1 liter: wait at least 2.5 hours
- For open vessels containing up to 2 liters: wait at least 3 hours
- Do not remove flasks or bags until the chamber temperature drops below
70C.
Disconnect the power supply plug if the unit is not going to be used for an
extended period of time.

Step back when opening the sterilizer.

Do not put any objects insides.

When an object that is easy to be combustible is like a stone, a metal or paper
goes insides, it may cause a fire, an electric shock or a break down.
If do, pull out the mains plug from a socket and contact a manufacturer or a
supplier.
Do not disassemble or remodeling by
yourself. It may cause damage to the
designed safety.
We do not have any duties for an accident, quality deterioration or a defect caused
by a customer.
All the mechanisms and electric/electronic devices in this machine are designed
and developed by the manufacturer. The devices could be disassembled and
serviced only by the engineers of manufacturer or by engineers approved by
manufacturer.
Please avoid ultrasonic lamps.
The unit could be cracked or faded.

Watch for hot steam and hot water.

Hot steam may escape when the lid is opened.

The lid handle should only be gripped at the experiment end.
Do not use outdoors avoid overheating or electrical faults. The use in the place
where the sun continuously shines and can get rained on it is prohibited. It may
cause overheating or electrical fault of the product.
 Please do not use flammable or volatile products in the vicinity of the product to
minimize the risk of firer. Please do not use the flammable spray such as lacquers
and paint, or volatile, flammable chemicals and its similar product, in the
Proximity of the product. They may cause the ignition due to the spark of switch.

This product must be connected to a properly grounded power supply.

Only operate unit after checking the safety of the unit.

If unexpected sound, smell or smog is generated, pull the main plug out from the
wall socket and contact the technical assets of DAIHAN Scientific

Do not touches the device with wet hand while operation.

Do not turn on and off frequently. It could damage the compressor by overload.

Do not use tap water!

The following products are not suitable for sterilization;

 Wooden items
 Bonded parts
 Plastic materials sensitive to hot water (e.g., screw tops)
 Electrical equipment
 Vessels or instruments made from steel or iron
 Metering equipment, unless their suitability for stream sterilization is
specifically mentioned
Do not open sterilizing chamber until full sterilization operation cycle is finished.
When sterilizing liquids, do not fill vessels more than two-thirds full, as otherwise
liquids may boil over.
Do not sterilize pressure-sealed vessels. Explosion hazard!
Watch for broken glasses!
Do not hang onto or lean against the open lid. DO not climb onto the autoclave,
as this may cause the autoclave to tilt.
This Symbol indicates that this product is to be collected separately.
The following apply only to users in European countries:
This product is designated for separate collection at an appropriate collection
point. Do not dispose of as household waste.
This product is required to comply with the European Union’s Waste Electrical &
Electronic Equipment(WEEE) Directive 2002/96/EC
※ All of the circuits, electrical or mechanical parts and configurations used in this product are
the technical assets of DAIHAN Scientific (Co., Ltd.). Only technicians of the technical support
team at DAIHAN Scientific (Co., Ltd.) or technicians approved through the education from
DAIHAN Scientific should be allowed to repair this product.
3. Product Usage
2.2 Names of Each Part

⑤ ⑥
 Outside

③
④

②
①

<Fig.3.2.2> Back of "MaXterileTM 47 /60"

⑦
< Figure 3.2.3>Back of "MaXterileTM 80 /100"

< Figure 3.2.1> Steam Sterilizers Digital Fuzzy-controlled Autoclaves,

" MaXterileTM 80 "

NO Names Description
① Leakage Breaker Circuit Breaker
② Power Switch Power ON/OFF
③ Lever Door Safety Lock and easy open
④ Lid Clamp Door Locking
⑤ Control Panel LCD Display
⑥ Pressure Gauge manometer
⑧ Moving Wheel Transport Device

<Table.3.2.1> Names of each part and description

 Inside

⑩
⑧

< Figure 3.2.4> Side of " MaXterileTM " < Figure 3.2.5> Inside of " MaXterileTM "

NO Names Description
⑧ Door Fastener Door locking mechanism
⑨ Safety Knob Prevent overheat
⑩ Manual purge valve pressure pass Control Device
⑪ Water Drain Valve Drain Control Device
⑫ Condenser emission steam condensation mechanism

<Table.3.2.2> Names of each part and description

 Controller

②
①
Time SV 00:00
Time PV 00:00

③⑥

<Figure 3.2.6> Controller for Steam Sterilizers Digital Fuzzy-controlled Autoclaves, " MaXterileTM "

NO Names Description
① LCD Display Set-up Value and Current Status.
② LED Lamp Indicate operation of TIMER, HEAT, ERROR.
③ MAIN Button Convert into mode of Timer, Heating, Printer, Locking.
④ Convert into mode of delay timer, offset function, Setting
SUB Button
Operating Information.
⑤ OPEN Door open.
⑥ Control that Reduces or Adds Values, and Does Start/Stop
Jog Dial knob
Operation.

<Table 3.2.3> Controller for " MaXterileTM "

 3. Product Usage
2.3 Usage Instructions

- The SAFETY Knob is set for the user’s safety to the appropriate value at the time of the
factory shipment, so please do not change the Knob.
- When turning ON the power of this product, please turn ON the Main Power Switch,
after necessarily turning ON the Leakage Breaker located at the bottom of the rear
panel of this product.
- When the power is turned on, press the "OPEN" Button, and open the Door by pulling
the Lever.
- Check the level of distilled water in the pressure vessel, and please regulate to the level
that Heater Cover can be sunk.

Operation method for Jog-Dial

Jog-Dial can be manipulated for the setting in each mode by Turning and Push motion.

If turning the Jog-Dial knob to clockwise or counter-clockwise, the setting values can be adjusted, and if briefly
The Turning of the Jog-Dial knob does not manipulated during operating, and if resetting the set value, it can b
If pressing for 2 or 3 seconds the PUSH button, the set value is saved, and even if the power is turned on agai

① MAIN - Set Operation Time

- When the power is turned ON, the initial screen is the setting mode of the Timer set mode.

Timer LED (Green)

Set Time Time SV : 00:00
Time PV : 00:00
Remain Time
< Fig 3.3.1. Set Operation Time Mode >

- TIMER LED is lighted during the operation of timer, and indicates that it is operating.

- The range of setting time is from 00:00 to 99hr and 59min (it is possible to adjust at 1min.)
and 00:00 indicates continuous operation..
 - If the timer operation is completed, the alarm will sound and heating will be stopped.

- The timer setting mode can be operated by referring to the jog-shuttle operation method.

* SUB - Set Delay Time

- If pressing the SUB button in the Set Operation Timer Mode, it is switched to the Set
Delay Time mode.

- The Delay Timer sets the reservation time and has the function that sets so that the
operation of Heating and Timer are performed after that time is elapsed.
Timer LED (Green)
Set Time DTim SV : 00:00
DTim PV : 00:00
Remain Time

< Fig 3.3.2. Set Delay Time Mode>

- The proper use of the delay timer is to manipulate so that the reservation time is set for the
first time, and then the temperature and the operation timer is set and the set operation is
automatically operated after the reservation time is elapsed.

- The reservation time value is displayed over blinking on the display, and if pressing the
PUSH button by setting the reservation time value with the Jog-Dial knob, the value is fixed
on the Display, and the reservation timer is activated.

- After setting the reservation timer value, you can set-up the operation timer value by
pressing the MAIN button and then press Start button. And, you can set-up the temperature
by pressing the MAIN button again, and then press Start button. The Start button in the
condition that the reservation timer has been set-up, is the button for the operation of the
setting value after the reservation timer, not to immediately operate.

- As described above, if continuously setting the operation, Heating and Timer are operated
after the set reservation time as user intend.

- Since the delay timer is not set during the operation of Heating/Timer, the delay timer
should be set after stopping the operation of Heating/Timer.

- You can change or save the setting by using the operation method of the Jog-Dial, and
Start/Stop of operation can be performed.

- If pressing MAIN button, it is switched to the Set Operation Time Mode.

② MAIN - Set Temperature

- If pressing once the MAIN button in the Set Operation Time Mode, it is switched to the
temperature setting mode.

Set Temp
Temp SV : 0℃
Present Temp Heating LED (Red)
Temp PV : 27 ℃

< Fig 3.3.3. Set Temperature Mode >

- The setting temperature is entered by turning the Jog-Dial Knob to clockwise or counter-
clockwise. If the setting value is entered to above 0℃, the operation standby state is
displayed while blinking the HEATING indication of LCD Display.

- The HEATING indication of LCD Display is always displayed during the operation of
HEATING, and if the setting value is entered to above 0℃ in the condition that the heating
is stopped, the HEATING indication indicates the standby status while blinking in long
interval.

- The range of the setting temperature is adjustable in the unit of 1℃, and can be set up to
132℃.

- You can change or save the setting by using the operation method of the Jog-Dial, and
Start/Stop of operation can be performed.

* SUB - Offset Temperature

- If pressing the SUB button in the Set Temperature mode, it is switched to the Set
Temperature Offset mode.

- Temperature compensation is the function that can add or reduce the constant value by
setting the compensation value in the case that user must experiment by matching the
thermometer which he/she is using as the experimental standard and the thermometer
indicated in this regulator.

Present Temp Temp PV:27.0 ℃

Heating LED (Red)
offset:0.0 ℃
offset Temp

< Fig 3.3.4. Set offset value Mode>

- The scope of the compensative value is -10.0℃ ~ +0.0℃, and it is possible to adjust at 0.1℃
unit.
- After setting the compensation value, the indication of the current temperature is displayed
as the temperature including the compensation value.

- When entering into the compensation value setting mode during the Heating operation,
the Heating operation that is operating is stopped. After setting the compensation value,
the Heating operation is re-conducted in the Set Heating Mode.

- If the PUSH button of Jog Dial Knob is pressed and held for 2-3 seconds, the setting value
is saved and this saved value is maintained even when the power is turned on again.

- If pressing MAIN button, it is switched to the Set Temperature Mode.

③ MAIN - Safety Locking

- If pressing the MAIN button in the Set Temperature mode, it is switched to the Safety
Locking Mode.

Present Temp Temp PV: 27 ℃

TimePV : 00:00
Remain Time
< Fig 3.3.5. Safety Locking >

- In the Locking Mode, the Turning by the Jog-Dial Knob or Push button do not applied, and
LCD Display and LED lamp indicate the status that is currently operating.

- If pressing once the MAIN button, it is entered into the Set Operation Time Mode.

* SUB - Set Operation Cycle

- If pressing the SUB button in the Safety Locking mode, it is switched to the Set Operation
Cycle Mode.

Operation Mode SOLIDCycle Operation Mode LIQUIDCycle

< Fig 3.3.6. SOLID Cycle Mode > < Fig 3.3.7. LIQUID Cycle Mode >

- The operation mode can be selected among the SOLID cycle mode that operates for the
sterilization of solids or dried samples, and the LIQUID cycle mode that performs the
sterilization operation of solutions.
- This product has been set up to the SOLID Cycle mode in the factory shipment, and the
user can select among SOLID and LIQUID by pressing the Jog-Dial PUSH button. If
pressing and holding the PUSH button, the operation mode that the user wants to set is
saved.

* Sterilization operation and End of Sterilization

SOLIDCycle Heating.. < Fig 3.3.8. Heating Operation >

V/O
V/O = Valve Open
V/C = Valve Close

SOLIDCycle sterilization..
< Fig 3.3.9. sterilization Operation >
V/C

SOLIDCycle Exhaust..
< Fig 3.3.10. Exhaust Operation >
V/O

- In each mode, the screen appears alternately depending on the sterilization process.

- If the sterilization operation is completed, the heating operation is stopped, and the
steam is safely emitted through the emission steam condensation mechanism.

- In the case of the SOLID Cycle mode, the steam is rapidly and safely emitted through the
emission steam condensation mechanism.

- In LIQUID cycle mode, valve is not completely opened at a time. Firstly it waits for 5
minutes with valve closed after heater OFF. And then, the valve is opened during 3
seconds, after that the valve is closed again for 2 minutes, and then it reopens during 3
seconds, etc. This procedure repeats until the temperature comes down to 90°C (lasts
approx. 30minutes), and then it waits until the temperature reaches down to 80°C with
valve closed again. After that, the valve is closed completely. This is the operation
principle that a sudden change in pressure can be prevented and the samples of liquid
material can be safely protected.
* After Sterilization

Temp SV : 120 ℃ Temp SV:120 ℃

Temp PV : 79 ℃ Open the Door !!!

< Fig 3.3.11. Door Open Message>

- If the sterilization operation is completed, all steam is safely emitted, and if the Open the
Door Message is appeared on the LCD Display along with Buzzer sound, the user can safely
take out the Sample. (If less than 80 ℃ Message appears.)
- Door Open Message Display will continue until the Open Door.
(when the Pressure "0", temperature "80 ℃" "Door Open" message will appear. Please be
careful it may be hot in the internal temperature.)
- After checking that the pressure of the mounted pressure gauge is zero (0), press the Open
button and please open the door by pulling the Lever.
- To check the perfect pressure ejection of the pressure vessel before opening the door of
the pressure vessel, the user open the Manual purge valve and can finally check.
- If the user wants to remove the remaining distilled water in the pressure vessel, it can be
removed by opening the Water Drain Valve. Please check the device which will collect the
drained distilled water, before opening the valve.

* Error Message
ERROR Message Cause Remedy
Informs the time to be displayed the
Please check the setting
Error 1 temperature of abnormal value due
to temperature.
the abnormality of Temperature
Sensor..
The mounted inner water level
sensor informs to user when Please check the amount of
Error 2
the internal distilled water.
distilled water is not filled up to
Heater.
Inform when can not operate owing Please check the amount of
Error 3
to Opened Door. internal distilled water.

* Emergency Lid unlock method

autoclaves has electronic lid lock system for customer’s safety. So, if customer face power
failure or something wrong in power supplier of units, customer couldn’t open the lid by push
the “Open” button. In this case, for opening the lid, please do below procedure.
 Hall for Emergency Lid unlock method

< Fig 3.3.12. Lid unlock method >

- There is the Lid unlocked hole at the central axis part of Lid in an emergency. Please insert
the appropriate sized tool (hexagonal wrench, etc.) in this hole.
- Check the heard sound when the closing and opening device is opened, and please lift up
the Lid with the inserted status of tool maintained.
- Please open the Lid up to the end while slowly extracting the tool with keeping the force
lifting up the lid.

4. Products Management
4.1 Accessories 4
* Ordering Information

Ordering
Image Information
Number

Perforated Basket, Φ270 x h250mm, "WAC20047", for 47Lit.

DH.WAC20047
" MaXterileTM 47"," MaXterileTM 47R"

Perforated Basket, Φ320 x h250mm, "WAC20060", for 60Lit.

DH.WAC20060
" MaXterileTM 60"," MaXterileTM 60R"

Perforated Basket, Φ370 x h250mm, "WAC20080", for 80Lit.

DH.WAC20080
" MaXterileTM 80"," MaXterileTM 80R"

Perforated Basket, Φ420 x h250mm, "WAC20100", for 100Lit.

DH.WAC20100
" MaXterileTM 100"," MaXterileTM 100R"

DH.WAC30047 Validation Service (lQ, OQ), "ACV47", for "" MaXterileTM 47"

Validation(lQ, OQ) DH.WAC30060 Validation Service (lQ, OQ), "ACV60", for "" MaXterileTM 60"

Service DH.WAC30080 Validation Service (lQ, OQ), "ACV80", for "" MaXterileTM 80"

DH.WAC30100 Validation Service (lQ, OQ), "ACV100", for "" MaXterileTM 100"
4. Products Management Method
4.2 Maintenance and Management

Steam Sterilizers, Digital Fuzzy-controlled Autoclaves was manufactured with the consideration
of various situations that may occur during the operation. However, for long-term use with
stable performance, please observe the following precautions:

 If the unit is not to be used for an extended period of time, disconnect the power
cord from the wall socket.
 DAIHAN is not responsible for damage to the unit caused by abnormal use or by not
heeding warnings and cautions in the manual or unauthorized modifications of the unit.
 The Air Duct is controlled by user’s needs, and if it is unused, it should be closed the
entrance and then stored in order to prevent the external contaminants entering from
the entrance.

CLEANING
 Before cleaning the unit, disconnect the power cord from the wall socket. Otherwise, it may
cause an electric shock or fire.
 To clean the unit, a neutral detergent and soft cloth is recommended. Do not use coarse
cloth, strong chemicals or organic solutions.
 Do not use water, Benzene, Thinner or any alcohol for cleaning the product. It may cause
discoloration, damage, an electric shock or fire.
 If you expect damage by a chemical during cleaning, call technical support team.
 Do not pour water directly into the unit. It may cause an electric shock or fire.
 Because the installed paper of the printer is the consumables, please start the operating
after checking the amount of the paper before the sterilization operation, and use the paper
according to the instructions when replenishing the paper.
 Please periodically clean the internal chamber according to the usage of the device. The
distilled water is discharged through the outlet, and then clean the chamber by pouring the
warm tap water into it. Thoroughly emit the tap water used for cleaning after wiping the
inside of the chamber with the cloth.
 Wire, brush, steel cotton, abrasives, or the cleaning goods containing a chloride should not
be used, when cleaning the inside of the product. Please necessarily use the cloth such as
towels.
4. Products Management
4.3 If product Defect Occur

Situation Confirmations and Solutions

Please check the connection of the power switch, and if

If power does not turn ON. there are not any troubles, check the power supply in the
installation site of the device.

If the temperature does not rise at Check the temperature currently set.

room temperature. Make sure that the power switch is in ON position.

Make sure that the Push button of the Jog Dial knob is

exactly pressed to operate the set temperature.

If the temperature is risen but the The abnormality may be present in the built-in sensor or the

heater does not operate to the set main control unit.

temperature. Please contact to the service center.

※ If you have any problem other than the above, please call technical service of manufacturer
or an official agent.