Octet BLI Biosensor

Selection Guide
 Octet® BLI System Quantitation Dynamic Range1
Octet ®
Description Intended Application Octet® QKe‡, Octet® RED96e‡, K2‡, Octet® N1 Regeneration
Consumables Use2 QK384‡, RH96 R2, R4, R8, RH16,
≥32 Channel RH96
8 or 16 Channel
Biosensors
AHC Anti-Human K Capturing human IgG’s or human N/A N/A N/A Yes for K
(Cat. Nos. 18-5060, Fc‑Capture Fc‑fusion proteins for kinetic analysis
18-5063, 18-5064)
with various analytes
AHQ Anti-Human Q Quantitation measurements of human IgG’s 0.025–200 µg/mL 0.01–200 µg/mL 0.25–500 µg/mL No for Q
(Cat. Nos. 18-5001, IgG Fc or human Fc-fusion proteins
18-5004, 18-5005)

AHC2 Anti-Human Q and K Capturing human IgG’s or human Fc-fusion 0.5-2000 µg/mL 0.1-2000 µg/mL 0.5-4000 µg/mL Yes for Q and K
(Cat. Nos. 18-5142, Fc-Capture proteins for both kinetic and quantitation
18-5143, 18-5144)
2nd Generation analysis
AMC Anti-Mouse K Capturing mouse IgG’s or mouse N/A N/A N/A Yes for K
(Cat. Nos. 18-5088, Fc‑Capture Fc‑fusion proteins for kinetic analysis
18-5089, 18-5090)
with various analytes
AMQ Anti-Murine IgG Fv Q Quantitation measurements of mouse 0.05-200 µg/mL 0.025-200 µg/mL 0.5-500 µg/mL No for Q
(Cat. Nos. 18-5022, IgG’s or mouse F(ab’)2
18-5023, 18-5024)

APS Aminopropylsilane K Binding measurement of lipids, liposomes, N/A N/A N/A Protein and analyte
(Cat. Nos. 18-5045, hydrophobic proteins that don’t have other dependent, users should
18-5046, 18-5047)
methods of surface attachment validate their own assays
AR2G Amine Reactive 2G K Covalently immobilizing any molecule with a N/A N/A N/A Protein
(Cat. Nos. 18-5092, terminal amine group for all kinetic analyses dependent
18-5093, 18-5094)

FAB2G Anti-Human Q and K Kinetic analysis of human Fab fragments Analyte dependent, Analyte dependent, Analyte dependent, Yes for Q and K
(Cat. Nos. 18-5125, Fab-CH1 2nd and IgG with target antigen, Fc receptors, or typically typically typically
18-5126, 18-5127)
Generation other analytes. Quantitation of Fab and IgG. 0.5–1000 μg/mL 0.5–1000 μg/mL 0.5–1000 μg/mL
GST Anti-GST Q and K Quantitation of GST-tagged proteins, direct Protein dependent, Protein dependent, Protein dependent, Yes for K, no for Q
(Cat. Nos. 18-5096, capturing of GST-tagged proteins for kinetic typically typically typically
18-5097, 18-5098)
analyses with analytes 0.1–2000 µg/mL 0.1–2000 µg/mL 0.5–1000 µg/mL**
HIS1K Anti-Penta-HIS Q and K Capture of His-tagged proteins for kinetic Protein dependent, Protein dependent, Protein dependent, Yes for K
(Cat. Nos. 18-5120, analysis with target analytes. Quantitation typically typically typically
18-5121, 18-5122)
of His-tagged proteins in buffer, media or 0.25–200 µg/mL* 0.25–200 µg/mL* 10–200 µg/mL*
diluted lysate. Biosensor is pre-coated with
Penta-His antibody from Qiagen.
HIS2 Anti-HIS Q Quantitation of HIS-tagged proteins in Protein and protocol Protein and protocol Protein dependent, Protein
(Cat. Nos. 18-5114, crude matrices or buffer or column eluent (time and rpm) (time and rpm) typically dependent
18-5115, 18-5116)
(pre‑coated with anti-His Ab from MBS) dependent, dependent, 0.1–200 µg/mL**
0.1–200 µg/mL** 0.1–200 µg/mL**
1
Dynamic range might vary for different background conditions, numbers listed are guidelines only and are based on testing of intended analyte molecules, users should validate range for their own samples
2
Biosensors are developed, manufactured, and QC is performed for their intended applications; using biosensors outside their intended purpose requires user validation
 Octet® BLI System Quantitation Dynamic Range1
Octet Biosensor Description
®
Intended Application Octet® QKe‡, Octet® RED96e‡, K2‡, Octet® N1 Regeneration
Use2 QK384‡, RH96 R2, R4, R8, RH16, RH96
≥32 Channel 8 or 16 Channel
NTA Ni-NTA Q and K Quantitation of HIS-tagged proteins in Protein dependent, Protein dependent, Protein dependent, Yes for K, no for Q
(Cat. Nos. 18-5101, buffer or diluted matrix, capturing of typically typically typically
18-5102, 18-5103)
HIS-tagged proteins for kinetic analyses 0.5–1000 µg/mL 0.5–1000 µg/mL 0.5–1000 µg/mL
with various analytes
ProA Protein A Q Quantitation of IgG’s of various species 0.1–700 µg/mL 0.025–2000 µg/mL 0.5–4000 µg/mL Yes
(Cat. Nos. 18-5010, including human
18-5012, 18-5013)

ProG Protein G Q Quantitation of IgG’s of various species 0.1–700 µg/mL 0.025–2000 µg/mL 0.5–4000 µg/mL Yes
(Cat. Nos. 18-5082, including human
18-5083, 18-5084)

ProL Protein L Q Quantitation of IgG’s of various species via 0.1–700 µg/mL 0.05–2000 µg/mL 0.5–2000 µg/mL Yes
(Cat. Nos. 18-5085, the kappa light chain
18-5086, 18-5087)

SA Streptavidin K Immobilizing biotinylated molecules for all N/A N/A N/A Protein
(Cat. Nos. 18-5019, kinetic analyses dependent
18-5020, 18-5021)

SAX High Precision Q and K Immobilizing biotinylated molecules for high Protein Protein Protein Protein
(Cat. Nos. 18-5117, Streptavidin precision quantitation and kinetic dependent dependent dependent dependent
18-5118, 18-5119)
measurements
SAX2 High Precision Q and K Immobilizing biotinylated molecules for Protein Protein Protein Protein
(Cat. Nos. 18-5136, Streptavidin 2.0 high precision and reproducible kinetic dependent dependent dependent dependent
18-5137, 18-5138)
characterization and custom quantitation
SSA Super Streptavidin K Small molecule and fragment analyses N/A N/A N/A Analyte compounds can
(Cat. Nos. 18-5057, only, should not be used for large be washed off during dis­
18-5065, 18-5070)
molecule measurements sociation in buffer since
most have fast offrates
AAVX AAV Quantitation Q Quantitation of AAV Capsids for various AAV AAV serotype and AAV serotype and AAV serotype and Yes
(Cat. Nos. 18-5160, serotypes, including AAV1 - AAV9 and sample dependent, sample dependent, sample dependent,
18-5161, 18-5162)
AAVrh10 typically 8.5E8-1.0E13 typically 8.5E8 - typically 8.5E8 -
vp/mL 1.0E13 vp/mL 1.0E13 vp/mL
Kits
AR2G Amine Coupling K Reagent kit for immobilizing any molecule N/A N/A N/A Protein dependent
(Cat. No. 18-5095) 2nd Generation with a terminal amine group onto Octet®
Reagent Kit AR2G biosensors
GlyM Mannose Screening Q Relative screening of Mannose glycans in Sample Sample Sample No for Q
(Cat. No. 18-5139) Kit crude or purified cell culture samples dependent dependent dependent
GlyS Sialic Acid Q Relative screening of sialic acid in crude or Sample Sample Sample No for Q
(Cat. No. 18-5135) Screening Kit purified cell culture samples dependent dependent dependent
HCP Anti-CHO HCP Q High sensitivity assay kit for generic analyses Sample dependent, Sample dependent, Sample dependent, No for Q
(Cat. Nos. 18-5141, Detection Kit of CHO HCP typically typically typically
18-5158)
0.5–200 ng/mL 0.5–200 ng/mL 0.5–200 ng/mL
RPA Residual Protein A Q High sensitivity assay kit for analyses of Sample dependent, Sample dependent, Sample dependent, No for Q
(Cat. No. 18-5128) Detection Kit residual Protein A typically 0.1–25 ng/mL typically 0.1–25 ng/mL typically 0.1–25 ng/mL
* Assay conditions and dynamic range should be validated ** Users should validate their assay ‡
Discontinued model
Germany USA
Sartorius Lab Instruments GmbH & Co. KG Sartorius Corporation
Otto-Brenner-Strasse 20 565 Johnson Avenue
37079 Goettingen Bohemia, NY 11716
Phone +49 551 308 0 Phone +1 888 OCTET 75
Or +1 650 322 1360

 F
 or further contacts, visit
www.sartorius.com/octet-support

Specifications subject to change without notice.