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Practical Outline of Medical Cell Biology For Nepalese Students

This document outlines a practical course in Medical Cell Biology for Nepalese students. It consists of 4 practical sessions that cover: 1) the morphological structure of cells, 2) using a micrometer to measure cells, 3) cytochemistry and cytophysiology techniques to observe cell components, and 4) observing cell mitosis in plant and animal cells. Each practical has objectives, content that will be covered, and homework assignments, such as making drawings of cells and labeling structures. The course aims to teach students skills in cell observation, measurement, staining, and the stages of mitosis.

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185 views2 pages

Practical Outline of Medical Cell Biology For Nepalese Students

This document outlines a practical course in Medical Cell Biology for Nepalese students. It consists of 4 practical sessions that cover: 1) the morphological structure of cells, 2) using a micrometer to measure cells, 3) cytochemistry and cytophysiology techniques to observe cell components, and 4) observing cell mitosis in plant and animal cells. Each practical has objectives, content that will be covered, and homework assignments, such as making drawings of cells and labeling structures. The course aims to teach students skills in cell observation, measurement, staining, and the stages of mitosis.

Uploaded by

api-3728690
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Practical outline of Medical Cell Biology for Nepalese Students

No Contents period
Practical 1 Morphological Structure of Cell 3 hours
Practical 2 Micrometer of the cell 3 hours
Practical 3 Cytochemistry and Cytophysiology 3 hours
Practical 4 Cell Mitosis 3 hours

Practical outline:
Practical 1 Morphological Structure of Cell
I. Objective:
⒈The student will master the following skills:
⑴ preparation of cells for examination on the microscope
⑵ staining cells with standard stains
⑶ setting up the microscope for contrast enhancement
⒉ The student will be able to:
⑴ acquaint with the structure of light microscope
⑵ Learn proper handling and become familiar with the use of light microscopes for biological
investigation.
⑶ Master the morphological structure character of cell under the light microscope.

II. Content:
⒈ The usage of optical microscope
⒉ Practise using general optical microscope and observe differient type of tissue cell.
⒊ The main practical slides are provided with:
⑴ human blood smear
⑵ toad blood smear
⑶ mouse small intestine pillar epidermal cell
⑷ skeleton muscle slide
⒋ Manipulation
Make onion epidermal cell temporarily slide
III. Homework:
⒈ Please write down the procedure of the manipulation.
⒉ Make drawing of the onion epidermal cell and label the structure.

Practical 2 Micrometer of the cell


I. Objective
Master the principle and the usage of micrometer
II. Content:
Micrometry the toad red blood cell
III. Homework:
⒈ the calibration of your ocular micrometer (10x,40x)
⒉ the radius (a and b) of the toad red blood cell and the nuclear ,use your 40x objective lens.
⒊ Calculate the volume of the cell and the nucleoplasmic ratio (NPR)

Practical 3 Cytochemistry and Cytophysiology


I. Objective
I: observe some kinds of chemical components and their distribution in cells.
II: master methyl green-pyronin staining method to show nucleic acid.

II. Content:
⒈ Observation of cellular chemistry composition by optical microscope

1
⑴ glycogen---animal liver smear, PAS reaction
⑵ acid protein---toad blood smear, acid fixed green staining
⑶ alkaline protein---toad blood smear, alkaline acid fixed green staining
⑷ DNA--- animal liver smear, fulgen staining
⑸ lipid---animal liver smear, Sudan Ⅲ staining
⑹ Alkaline phosphoric acid kinase---mouse kidney slide
⒉ Manipulation
Make toad blood smear, using methyl green-pyronin method to show DNA and RNA.

III. Homework:
⒈ Procedure of the manipulation.
⒉ Make drawing of the toad blood smear to show the position of the DNA and RNA.

Practical 4 Cell Mitosis


I. Objective
⒈ Be familiar with the stages of both mitosis and meiosis
⒉ Be able to identify the stages of mitosis in both animal and plant cells via the microscope
II. Content:
⒈ Observation by the microscope:
⑴ amitosis---paramecium enclosed slide
⑵ mitosis of plant cell---the slit slice of onion root tip
⑶ mitosis of animal cell---equine ascarid zygote slice
⒉ meosis---photograph of the grasshopper spermary
III. Homework:
Please make drawing of the mitosis of the onion root tip cell to show the character of the
prophase, metaphase, anaphase, telophase.

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