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Biology Pratical

The document discusses an experiment investigating catalase activity in cooked and uncooked potato tissue. Catalase is an enzyme that breaks down hydrogen peroxide, producing oxygen which forms a foam. The student measured the height of the foam produced by potato cylinders that were either cooked or uncooked to see if cooking affected catalase activity.

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Zaw Linn Phyo
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33 views6 pages

Biology Pratical

The document discusses an experiment investigating catalase activity in cooked and uncooked potato tissue. Catalase is an enzyme that breaks down hydrogen peroxide, producing oxygen which forms a foam. The student measured the height of the foam produced by potato cylinders that were either cooked or uncooked to see if cooking affected catalase activity.

Uploaded by

Zaw Linn Phyo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Biology

Alternative to Practical

Marks - _______/20
1. Catalase is an enzyme found in plant and animal cells. It catalyses the breakdown of
hydrogen peroxide to form water and oxygen.

The oxygen produced forms a foam. You can measure the height of the foam to determine
catalase
activity.
A student investigated catalase activity in cooked and uncooked potato tissue.
The student:
Step 1 cut two potato cylinders so that they were identical in shape and size
Step 2 put 5 cm3 of 3% hydrogen peroxide solution into a test-tube labelled cooked potato
Step 3 put 5 cm3 of 3% hydrogen peroxide solution into a test-tube labelled uncooked potato

Step 4 put one of the potato cylinders into a beaker of hot water for five minutes

Step 5 removed the potato cylinder from the hot water and put it into the test-tube labelled
cooked potato

Step 6 put the uncooked potato cylinder into the test-tube labelled uncooked potato

Step 7 left the potato cylinders in the 3% hydrogen peroxide solution for three minutes and then
measured the height of the foam produced in each of the test-tubes.
(a) (i) Prepare a table to record your results.
Measure the height of the foam in each of the test-tubes in Fig. 1.1. Record these
measurements in your table. [3]

(ii) Calculate the difference in the height of the foam produced by the cooked and uncooked
potato after three minutes. [1]

(iii) State one conclusion for these results. [1]

(b) (i) Identify the independent variable in this investigation. [1]

(ii) State why it is important that the two potato cylinders were identical in shape and size.[1]
(iii) State two other variables that were kept constant in this investigation. [1]

(c) Identify one possible source of error in the method used in this investigation. [1]

(d) A student stated that:

‘Catalase activity is the same in all species of plants.’

Plan an investigation to test this statement. [5]


(e) Potatoes contain starch. Starch can be broken down into reducing sugars.
Describe the tests to identify starch and reducing sugars and give the results of the positive
tests. [5]

Starch

Reducing sugars

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