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ST. BERNADETTE OF LOURDES COLLEGE

COLLEGE OF NURSING
MICROBIOLOGY AND PARASITOLOGY
LABORATORY WORKSHEET
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THE COMPOUND MICROSCOPE
Name: JUNAID, KATRINA L. ________ Score: __________________
Year & Sec: 3RD/ SECOND COURSER Date:

INTRODUCTION

Microbiology is the study of microscopic living creatures such as viruses, bacteria, fungus, and parasites. Because
these creatures are undetectable to the naked eye, a microscope is a very useful instrument for seeing them. A
compound microscope is a type of optical equipment. It's used to magnify tiny items. It has two lenses: the objective
lens and the ocular or eyepiece lens. The magnification is the number of times an item has been magnified, and the total
magnification is calculated by multiplying the magnifying power of the objective lens by the magnifying power of the
ocular.

A microscope's resolving power, often known as resolution, is an essential component in addition to

magnification. Resolution is the capacity of the lenses to recognize two different things at a given distance away as two
distinct objects. A compound microscope has a resolution of 0.2 um, which is 1,000 times greater than the human eye.

RESULTS AND DISCUSSION

Label the parts of a compound microscope as shown below.

Eyepiece(Ocular lens)

Head

Arm(Carrying Handle) Nose piece

Objective Lens
Stage Clip
Mechanical Stage
Coarse Adjustment
Condenser
Fine Adjustment

Illuminator

Base
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1. What are the different parts of the microscope? Complete the table below.

Mechanical Parts Function

1. Foot or Base

2. Pillar

3. Arm

4. Stage

5. Inclination joint

6. Clips

7. Diaphragm

8. Nose piece

9. Body tube

10. Fine adjustment knob

11. Coarse adjustment knob

Magnifying Parts Function

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Illuminating Parts Function

QUESTIONS FOR RESEARCH

1. Define the following terms used in microscopy.

Parfocal
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Working distance

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Field of view

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Micrometer

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2. How can eye strain be avoided in using the microscope?

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3. When is the oil immersion objective used? What kind of oil is used?

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