HYSILK ®

HySilk is hyaluronic acid a low molecular weight and has a very broad range of activity in the skin. It works both to
change the physical properties of the epidermis, especially due to its ability to immobilize a large volume of water in
its structures, and is also active in relation to cells, so it is capable of acting positively on intercellular communication.

Support of epidermal hydration by Reduced oiliness by controlling

improving intercellular communication the immune response
112 120

110
100
108

106 80
% of control

of 0 week
104
% of control

60

week
102

% of %
100 40

98
20
96

94 0
Control HySilk 0 week 4 weeks 8 weeks 12 weeks

Hydration promotion after HySilk treatment, 8 volunteers treated with 0.005% HySilk (24-43 years) + 29 volunteers Oiliness reduction after HySilk treatment, 8 volunteers treated with 0.005% HySilk (24-43 years) + 29 volunteers
control group, Daily application for 4 weeks, p ≤ 0.05, Measured by MPA 580, Corneometry control group, Daily application for 12 weeks, p ≤ 0.05, Measured by MPA 580, Glossymetry

During the in-vivo Although an oily film on the surface of the skin helps to reduce tran-
of the upper layers of the epidermis was observed, manifested by sepidermal water loss (TEWL), excessively oily and very shiny skin
lower transepidermal water loss (TEWL) and higher water content. is not aesthetically pleasing. HySilk has shown an ability to reduce
the oiliness of skin. During an in vivo study on volunteers treated
HySilk’s ability to partially penetrate into the skin has been
with 0.005% HySilk cream, more than 20% reduction of oiliness was
demonstrated, in view of its moisturizing qualities, to make a major
seen in 12 weeks.
contribution to increased water content in the stratum corneum.
Compared with the control group, hydration rose by 10%.

Mechanisms of action Mechanisms of action

174
120

172 100

80
170
of control
% of control

60
% of control

% control

168
40
% of

166
20

164 0
Control 390 570 660 740
162 -20
SPRR1B SPRR2 MW of HySilk (kDa)

Changes in gene expression in HaCaT keratinocytes after 0.1% HySilk 48 hours treatment, n=1, Measured by DNA TNF-alpha production from keratinocyte primoculture 24 hours after UV radiation, 5 mJ/cm² UVB, 0.05% HySilk treat-
array ment, n=1, Measured by ELISA

HySilk’s basic mechanism of action is the improved barrier func- The oily film on the skin’s surface is formed by sebocytes in in skin
tion of the stratum corneum. HySilk’s molecular weight enables it glands; one job is to help reduce TEWL. The barrier function and
to penetrate into the deep layers of the stratum corneum in just a adequate hydration may, however, replace the function of oily film to
matter of hours, where it operates on several levels. First, it has the some extent, rendering its excessive production unnecessary.
capacity to protect phospholipids against peroxidation by UV radia-
It is known that proinflammatory cytokines, particularly tumor ne-
tion and free radicals. These phospholipids are part of the natu-
crosis factor TNF-alpha and prostaglandin E2 (PGE2), trigger the
ral moisturizing factor (NMF), the presence of which is essential for
production of sebum in sebocytes1. A state of chronic inflammation,
the proper barrier function of the skin.
a deviation of the immune system characterized by the increased
- production of these substances, is common in premature aging
cocerebrosidase, an enzyme responsible for the release of cera- skin. 3
mides from their glucosyl precursors. Ceramides are then incorpo-
HySilk influences the TNF-alpha production, helping to stabilize the
rated into the lipid bilayer of corneocytes, and the barrier function
immune system in the skin.4 Decreased TNF-alpha production thus
is enhanced. 2

The last positive mechanism is the natural ability of hyaluronic acid

to immobilize a large quantity of water in its structures, which helps
to restore proper NMF functioning in the epidermis.

All data were obtained in the relevant in-vivo and in-vitro measurements and,
subject to registration, can be accessed at www.contipro.com/anti-aging
SPECIFICATION: HySilk®, powder

Origin biotechnological processing

Appearance white to slightly yellow powder or granules

Appearance of 0.5% aqueous solution clear to slightly opalescent, colourless solution

Clarity of 1% aqueous solution (660 nm, 1 cm) < 0.010

Loss on drying (%) ≤ 10.0

Molecular weight (SEC-MALS) 150 kDa – 1.3 MDa

pH of 0.5% aqueous solution 5.0 – 8.0

< 0.20

Microbial contamination (CFU/g) < 100

Sodium hyaluronate (%)* > 93.0

Uronic acids (%)* > 45.0

Ash (%)* < 10.0

* calc. on dry basis

SOURCE COMPATIBILITY
• by fermentation produced Hyaluronic acid with standard AND PROCESSING
molecular weight, additionally split by a controlled combination
•
decreasing molecular weight.
• non-GMO
• very sensitive to free radicals
• non-animal materials used during the manufacturing process
• incompatible with cationic substances,
e.g. quarternized polymers and proteins
SOLUBILITY
• fully soluble in water. Speed of dissolving depends on molecular
TOXICOLOGY
• soluble in a mixture of ethylalcohol and isopropylalcohol
• non-irritating
with water. Solubility depends on molecular weight and
• non-cytotoxic
concentration – the lower molecular weight, the better
solubility. • non-phototoxic

• soluble in mixture of propylene glycol and butylene glycol with

• insoluble in non-water miscible solvents

Literature:
1
Choi, J. J., M. Y. Park, et al. (2012). “TNF-alpha increases lipogenesis via JNK and PI3K/Akt pathways in SZ95 human sebocytes.”
J Dermatol Sci 65(3): 179-88

2
Redoules, D., R. Tarroux, et al. (1998). “Epidermal enzymes: their role in homeostasis and their relationships with dermatoses.” Skin Phar-
macol Appl Skin Physiol 11(4-5): 183-92

3
Thornfeldt, C. R. (2008). “Chronic inflammation is etiology of extrinsic aging.” J Cosmet Dermatol 7(1): 78-82

4
T.Muthny, M. Moravcova (2013). “Skin aging in the context of sun damage and immune response alterations.” SOFT Journal 4: 2-8

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Phone +420 465 520 035 Fax +420 465 524 098 E-mail sales@contipro.com
www.contipro.com Version: P-01/2014