Chap 2 F5 Biology
Chap 2 F5 Biology
2023) PG 26-57
1. Complete the diagram below to show the structure of a leaf.
2. Label the following diagram shows a cross section of a eudicot leaf lamina.
3. How does a thin and flat lamina allow the leaf to carry out photosynthesis effectively?
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5. State the difference between gaseous exchange in plants during respiration and photosynthesis.
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Uptake of potassium ions by guard cells Sucrose concentration in the guard cell sap
The potassium ions enter the …………………. In the presence of light, …………………s
The solute potential in the guard cells occurs
………….. The concentration of sucrose in the guard cells
The water potential in the guard cells becomes …………..
………….. The water potential in the guard cells ………….
The water molecules from the epidermal cells The water molecules from the epidermal cells
diffuse into the guard cells by ……………… …………. into the guard cells by osmosis
The guard cells become ………… and curve The guard cells become turgid and curve
outwards. ………….
The stoma opens. The stoma opens.
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
7. Explain the opening mechanism of stomata.
During the day, guard cells carry out ………………… to synthesis sugar at a fast rate.
………….. is generated for the active transport of potassium ions (K +) into the guard cells from the
neighbouring epidermal cells.
At the same time, sucrose is transported from the surrounding mesophyll cells into the
……………….
Higher solute potential causes the turgor pressure in the guard cells to ……………..
The water potential to ………….
The water from the surrounding epidermal cells diffuses into the guard cells through ……………..
Guard cells become …………… and swell up.
The guard cells curve outward, and the stoma ……………
Uptake of potassium ions by guard cells Sucrose concentration in the guard cell sap
The potassium ions move out from the In the absence of …………., photosynthesis
……………….. does not occur.
The solute potential in the guard cells The concentration of sucrose in the guard cells
…………. becomes ……………
The water potential in the guard cells …………. The water potential in the ……………
The water molecules …………. out from the increases.
guard cells to the epidermal cells by osmosis. The water molecules diffuses out from the
The guard cells become ……………. guard cells to the epidermal cells by
The stoma closes. ……………..
The guard cells become flaccid.
The stoma closes.
Extra notes
Stomata distribution on a leaf epidermis depends on the environmental conditions of the plant.
Terrestrial plants such as hibiscus plant have many stomata on the lower epidermis of their leaves.
Floating plants have more stomata on the upper epidermis of leaves.
Submerged plants do not have stoma.
Dessert plants have stomata embedded in epidermal surfaces.
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Kesan Kekurangan Air dalam Tumbuhan Terhadap Pebukaan dan Penutupan Stoma
Effect of Lack of Water in Plants to the Opening and Closing of Stomata TP 2
Lukiskan stoma dan labelkan. Apabila tumbuhan kekurangan air, sel pengawal
Draw the stoma and labelled. menjadi flasid
When the plant lacks water, theguard cells
become flaccid
Sifat dinding sel luar yang nipis dan lebih
elastic menyebabkan sel pengawal hilang
kesegahan dan stoma tertutup
The thin and more elastic outer cell wall causes
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
the guard cells to lose turgidity and the stoma to
close
Tumbuhan akan layu
The plants will wilted
Nota tambahan
Extra notes
Kira kira 90% transpirasi berlaku melalui stomata pada daun. Transpirasi juga berlaku melalui
lentisel pada tumbuhan berkayu.
About 90% of transpiration takes place through the stomata of the leaves. Transpiration also takes
place through the lenticels on woody stems.
The stomata of monocot plants are tiny pores in the upper and lower epidermis of the leaves, which
are surrounded by a pair of dumb-bell shaped guard cells.
Stomata tumbuhan monocot adalah berliang kecil di epidermis atas dan bawah daun, yang dikelilingi
oleh sepasang sel pengawal berbentuk ‘dumb-bell’.
The stomata of eudicot plants are tiny pores mainly in the lower epidermis of the leaves, which are
surrounded by a pair of bean-shaped guard cells.
Stomata tumbuhan eudicot adalah berliang kecil terutamanya di bahagian bawah daun epidermis,
yang dikelilingi oleh sepasang sel pengawal berbentuk kacang
9. Apakah kepentingan mempunyai lebih banyak stomata pada epidermis bawah daun?
What is the significance of having more stomata on the lower epidermis of the leaf?
……………………………………………………………………………………………………..
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10. Transpirasi berlaku melalui stomata pada daun. Transpirasi juga berlaku melalui lentisel pada
tumbuhan berkayu.
Transpiration occurs through the stomata on the leaves. Transpiration also occurs through lenticels
on woody plants.
13. Jelaskan apa yang berlaku pada stoma pada hari yang panas terik.
Explain what happens to stomata on a hot and scorching day.
……………………………………………………………………………………………………..
……………………………………………………………………………………………………..
TP 3
Proses Transpirasi
Transpiration Process
4. Penyejatan air dari sel mesofil menghilangkan …………….
Daripada daun. Ini memberi kesan …………… kepada tumbuhan.
The evaporation of water from the mesophyll cells removes ………
from the leaves. This produces a ……………… effect to plants.
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Keamatan cahaya
Light intensity
Pergerakan udara
Air movement Semakin tinggi kelajuan angin, semakin
…………… kadar transpirasi
The higher the wind speed, the …………….. the
Keadaan udara tidak bergerak, kadar rate of transpiration
transpirasi ………………..
In still air, the rate of transpiration Angin bertiup menyingkirkan wap air berdekatan
…………………… stoma. Ini menambahkkan ………………………
wap air di antara ruang udara dan persekitaran.
Wap air yang meresap keluar daripada Kadar resapan bertambah
daun akan ……………. di sekitar Wind carries away water vapour around the
stoma stomata. This will increase the ……………….. of
The water vapour that diffuses out of water vapour between the air space and the
the leaf will …………………… around surrounding air. The rate of diffusion increase
the stoma
Eksperimen 2.2 Kesan Faktor Persekitaran Terhadap Kadar Transpirasi dengan Menggunakan TP 4
Experiment 2.2 Potometer
The Effects of Environmental Factors on Rate of Transpiration Using a Potometer
Pernyataan Apakah kesan factor persekitaran terhadap kadar transpirasi?
masalah What are the effects of environmental factors on the rate of transpiration?
Problem
statement
Tujuan Mengkaji kesan factor persekitaran terhadap kadar transpirasi dengan mengunakan
Aim photometer
To study the effects of environmental factors on the rate of transpiration using a
potometer
Hipotesis a) Semakin tinggi pergerakan udara, semakin tinggi kadar transpirasi.
Hypothesis The higher the presence of wind, the higher the rate of transpiration.
b) Semakin tinggi keamatan cahaya, semakin tinggi kadar transpirasi.
The higher the light intensity, the higher the rate of transpiration.
c) Semakin tinggi suhu, semakin tinggi kadar transpirasi.
The higher the temperature, the higher the rate of transpiration.
d) Semakin tinggi kelembapan relative udara, semakin rendah kadar transpirasi.
The higher the relative air humidity, the lower the rate of transpiration.
Pemboleh ubah Dimanipulasikan :
Variables Manipulative
Bergerak balas :
Responding
Dimalarkan :
Constant
Bahan Ranting berdaun, air, jeli petroleum, kertas tisu, beg plastik
Materials Leafy twigs, water, petroleum jelly, tissue paper, plastic bag
Radas Gabus, bikar, jam randik, pembaris, kipas meja elektrik, meontol 100 W, photometer
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Apparatus (tiub kapilari, klip skru)
Cork, beaker, stopwatch, ruler, electrical table fan, 100 W bulb, photometer (capillary
tube, screw clip)
Prosedur 1. Sediakan photometer dan pastikan semua sambungan disapu dengan jeli
Procedure petroleum agar kedap udara.
Prepare a photometer and ensure the connections are applied with petroleum
jelly so that they are airtight.
2. Potong ranting tumbuhan yang berdaun di dalam air. Pastikan saiz batangnya
dengan gabus penutup photometer sepadan.
Cut a leafy twig of a plant in water. Ensure the size of the stem is compatible
with the cork covering the photometer.
3. Letakkan ranting berdaun pada photometer dan sapukan jeli petroleum agar
kedap udara.
Set the leafy twig to the photometer and make sure it is airtight.
4. Lap semua daun sehingga kering menggunakan kertas tisu.
Wipe all the leaves until they are dry using tissue papers.
5. Isikan air ke dalam photometer sehingga penuh.
Fill the photometer with water until it is full.
6. Perangkapkan gelembung udara kecil pada pangkal photometer untuk
digunakan sebagai penanda.
Trap a small air bubble at the base of the photometer to be used as an indicator.
7. Tandakan kedudukan awal gelembung udara dalam photometer sebagai P.
Mark the initial position of the air bubble in the photometer as P.
8. Dedahkan photometer di dalam bilik tanpa kipas berpusing.
Place the photometer in a room without fan circulation.
9. Selepas 10 minit, ukur jarak gelembung udara dalam photometer dengan
menggunakan pembaris atau skala yang ada pada tiub kapilari photometer.
After 10 minutes, measure the distance of the air bubble in the photometer
using a ruler or a scale on the capillary tube of the photometer.
10. Selepas itu, dedahkannya di hadapan kipas meja dengan kelajuan 2.
After that, placing it in front of fan with speed 2.
11. Selepas 10 minit, ukur jarak gelembung udara dalam photometer dengan
menggunakan pembaris atau skala yang ada pada tiub kapilari photometer.
After 10 minutes, measure the distance of the air bubble in the photometer
using a ruler or a scale on the capillary tube of the photometer.
12. Ulangi langkah 1 hingga 11 dengan mendedahkan set photometer di bawah
persekitaran yang berikut:
Repeat steps 1 to 11 by exposing the photometer set under the following
surroundings:
a) Keamatan cahaya yang tinggi dan rendah
High and low light intensities
(Di dalam bilik gelap dan di bawah mentol 100 W)
(In a dark room and under a 100 W bulb)
b) Suhu yang tinggi dan rendah
High and low temperatures
(Di dalam bilik dengan penyaman udara dan di dalam bilik tanpa
penyaman udara)
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
(In a room with an air conditioner and in a room without air conditioner)
c) Kelembapan relative udara yang tinggi dan rendah
High and low relative air humudities
(pokok ditutup dengan beg plastic lembap dan pokok yang tidak ditutup)
Plant that is covered with a humid plastic bag and plant that not covered)
13. Kira kadar transpirasi menggunakan formula berikut:
Calculate the rate of transpiration using the following formula:
Perbincangan 1. Ranting berdaun mesti dipotong di dalam air bagi mencegah ………. daripada
Discussion memasuki salur xylem untuk memastikan pengaloiran air di dalam xylem tidak
terputus.
Leafy branch must be cut in water to prevent ………. from entering its xylem
vessels to ensure that water flowed in the xylem was continuous.
2. Ranting berdaun ………… air untuk menggantikan air yang hilang melalui
transpirasi. Kadar penyerapan air adalah sama dengan kadar transpirasi.
The leafy branch …………. water to replace the water loss during
transpiration. The rate of water absorption is the same as the rate of
transpiration.
3. Apabila pergerakan udara laju, kadar transpirasi ………….. Pergerakan udara
yang laju akan menyingkirkan wap air yang terkumpul berdekatan dengan
permukaan daun. Kecerunan kepekatan wap air di dalam dan luar daun
bertambah.
When air movement increased, the rate of transpiration ……………. The rapid
air movement removes water vapour at the leaf surfaces. This increases the
gradient between the concentration of water vapour inside the leaves and the
concentration of water vapour outsidse the leaves.
4. Apabila keamatan cahaya ……………., kadar transpirasi bertambah. Cahaya
merangdsang pembukaan stoma menyebabkan lebih banyak air tersejat melalui
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
stoma.
When the light intensity …………….., the rate of transpiration increase. Light
stimulates the opening of stomata and causes more water to evaporate through
the stomata.
5. Apabila suhu bertambah, kadar transpirasi bertambah. Tenaga kinetic molekul
air meningkat. Kadar resapan air melalui stoma ……………….
When the temperature increase, the rate of transpiration increase. Kineteic
energy of water molecules increase. Diffusion rate of water through stomata
……………….
6. Apabila kelembapan relative udara bertambah, kadar transpirasi berkurangan.
Kelembapan udara yang …………… di persekitaran daun menyebabkan
kurang air tersejat melalui stoma.
When the relative air humidity increases, the rate of transpiration is decreases.
…………… air humidity in the environment reduces evaporation rate through
the stomata.
Kesimpulan 1. Semakin laju pergerakan udara, semakin tinggi kadar transpirasi.
Conclusion The higher the air movement, the higher the rate of transpiration.
2. Semakin tinggi keamatan cahaya, semakin tinggi kadar transpirasi.
The higher the light intensity, the higher the rate of transpiration.
3. Semakin tinggi suhu persekitaran, semakin tinggi kadar transpirasi.
The higher the temperature, the higher the rate of transpiration.
4. Semakin tinggi kelembapan relative udara, semakin rendah kadar transpirasi.
The higher the relative air humidity, the lower the rate of transpiration.
STANDARD KANDUNGAN / CONTENT STANDARDS
2.4 Organ Utama Fotosintesis
Main Organ for Photosynthesis
STANDARD PEMBELAJARAN / LEARNING STANDARDS
2.4.1 Mewajarkan keperluan fotosintesis dalam tumbuhan
Justify the necessity of photosynthesis in plants
2.4.2 Menghubung kait penyesuaian struktur dalaman daun dengan forosintesis
Correlate the adaptation of internal structure of leaf with photosynthesis
1. Tumbuhan adalah autotroph yang boleh menghasilkan makanan sendiri melalui ………………….
Plants are autotrophs that are able to make their own food through ………………….
2. Tumbuhan hijau menggunakan karbon dioksida dan air untuk mensintesis …………….. dan
membebaskan oksigen sebagai produk sampingan.
Green plants use carbon dioxide and water to synthesise ………….. and release oxygen as a by-
product.
3. Fotosintesis merupakan satu proses tumbuhan hijau mensintesis sebatian organic daripada karbon
dioksida dan air dengan kehadiran ………………..
Photosynthesis is a proses in which green plant synthesizes organic compounds from carbon dioxide
and water in the presence of ……………………
4. Melalui proses fotosntesis, tenaga cahaya matahari ……………. kepada tenaga kimia yang
tersimpan dalam molekul organic yang disintesiskan. Molekul organic dikenali sebagai glukosa ini
merupakan sumber tenaga kepada organisma lain.
Photosynthesis ………… light energy from the sun to chemical energy which is stored on organic
molecules that is synthesized. The organic molecules that are known as glucose are sources of
energy for other organisms.
5. Glukosa ini digunakan oleh organisma hidup yang lain untuk menjalankan …………… sel.
Glucose is used by other living organisms to carry out cellular …………………..
6. Semasa proses fotosintesis, tumbuhan hijau menggunakan karbon dioksida daripada udara dan
menghasilkan ……………. Gas oksigen ini kemudiannya digunakan oleh organisma lain untuk
proses respirasi sel.
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
During photosynthesis, green plants use atmospheric carbon dioxide and produce.……………. The
oxygen gas is then used by other organisms for cellular respiration.
Kutikel Kutikel adalah lapisan ……….. yang merangkumi epidermis atas dan bawah.
Cuticle The cuticle is a ……….. layer which covers the upper and lower epidermis.
Ia adalah penghalang kalis air untuk mengelakkan ……………… air yang
berlebihan melalui penyejatan.
It is a waterproof barrier to prevent excessive water ……. through evaporation.
Ia adalah lutsinar untuk membiarkan cahaya meresap ke bahagian dalam daun.
It is transparent to allow light to penetrate to the interior of the leaf.
Epidermis atas Epidermis atas daun terdiri daripada satu lapisan sel epidermis yang …………...
Upper epidermis The upper epidermis of a leaf consists of a single layer of epidermal cells which
are …………..
Sel-sel epidermis atas tidak mengandungi ………….. sehingga cahaya
menembusi jauh ke dalam lamina dan mencapai kloroplas dengan mudah.
The cells of the upper epidermis do not contain …………….. so light penetrates
deep into the lamina and reaches the chloroplasts easily.
Epidermis atas menutup dan melindungi permukaan atas daun dari kecederaan
fizikal, jangkitan dari mikroorganisma, dan mencegah kehilangan air yang
berlebihan.
The upper epidermis covers and protects the upper surface of leaves from
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
physical injuries, infections from microorganisms, and prevents excessive water
loss.
Berkas vascular Xylem mengalirkan air dan garam mineral dari ………. ke daun untuk
Vascular bundle fotosintesis.
Xylem conducts water and mineral salts from the …………s to the leaves for
photosynthesis.
Salur Xylem adalah saluran ……….. yang membantu laluan air.
Xylem vessels are ……….. channels that aid water passage.
Dinding salur xilem ditebalkan dengan lignin untuk memberi ………….. pada
daun dengan membiarkan daun tetap mendatar agar dapat menerima jumlah
cahaya matahari yang maksimum.
The walls of xylem vessels are thickened with lignin to provide ………….. to
the leaves by allowing the leaves to remain horizontal in order to receive the
maximum amount of sunlight.
Phloem mengangkut bahan hasil fotosintesis dari ……….. ke bahagian lain
tumbuhan. Ia membentuk tiub berterusan dari daun ke akar.
Phloem transports photosynthetic products from the …………. to other parts of
the plant. It forms a continuous tube from the leaves to the roots.
Mesofil palisad Sel mesofil palisade berbentuk silinder, rapat dalam susunan ……… tepat di
Palisade mesophyll bawah epidermis atas.
Palisade mesophyll cells are cylindrical, tightly packed in an ……………
arrangement directly beneath the upper epidermis.
Susunan sedemikian membolehkan sel-sel menerima jumlah cahaya matahari
yang ……………..
Such an arrangement allows the cells to receive the …………. amount of
sunlight.
Sel-sel ini mempunyai bilangan kloroplas yang …………. dan merupakan sel
fotosintesis yang paling aktif. 80% fotosintesis berlaku di sini.
These cells have a ……….. density of chloroplasts and are the most active
photosynthetic cells. 80% of photosynthesis takes place here.
Kloroplas dalam sel-sel ini dapat bergerak dan mengatur diri mereka untuk
melakukan penyerapan cahaya matahari maksimum.
The chloroplasts in these cells are able to move about and arrange themselves to
carry out the maximum absorption of sunlight.
…………… yang terkandung dalam kloroplas menyerap tenaga cahaya untuk
fotosintesis.
……………. contained in the chloroplasts absorbs light energy for
photosynthesis.
Dinding sel palisade dilapisi dengan lapisan air sehingga karbon dioksida dapat
larut dengan mudah dalam lapisan air sebelum meresap ke dalam sel.
The cell walls of palisades are coated with a film of water so that carbon dioxide
can dissolve easily in the film of water before diffusing into the cells.
Sel mesofil palisade mengandungi lebih banyak kloroplas daripada sel mesofil
spongy, dan oleh itu fungsi utamanya adalah fotosintesis.
Palisade mesophyll cells contain more chloroplasts than spongy mesophyll cells,
and therefore their main function is photosynthesis.
Mesofil berspan Sel mesofil berspan berbentuk tidak teratur dan disusun ……………. untuk
Spongy mesophyll meningkatkan luas permukaan dalaman untuk pertukaran gas di dalam daun.
Spongy mesophyll cells are irregularly shaped and …………. arranged to
increase the internal surface area for gaseous exchange within the leaf.
Sel mesofil berspan juga mengandungi kloroplas untuk melakukan fotosintesis.
Spongy mesophyll cells also contain chloroplasts to carry out photosynthesis.
Di antara sel-sel ini terdapat ruang …………. yang dihubungkan ke atmosfera
di luar daun melalui stomata.
Between these cells are ……… spaces which are linked to the atmosphere
outside the leaf through the stomata.
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Ruang udara yang besar mengurangkan jarak karbon dioksida yang meresap
melalui bahagian dalam daun untuk mencapai sel mesofil dan wap air untuk
meresap melalui stomata.
The large air spaces reduce the distance carbon dioxide has to diffuse through
the interior of the leaf to reach the mesophyll cells and water vapour to diffuse
through the stomata.
Dinding sel nipis yang dilapisi dengan lapisan air menyediakan permukaan
lembap agar karbon dioksida mudah larut dan mempercepat pertukaran gas
antara ruang udara dan mesofil berspan.
The thin cell walls which are coated with a film of water provide moist surfaces
for carbon dioxide to dissolve easily and to speed up gaseous exchange between
the air spaces and the spongy mesophyll.
Epidermis bawah Epidermis bawah mempunyai lebih banyak ……… berbanding epidermis atas.
Lower epidermis The lower epidermis has more ………….. compared to the upper epidermis.
Sel epidermis tidak mengandungi ………….. kecuali sel pengawal yang
merupakan sel epidermis khusus.
Epidermal cells do not contain …………. except for guard cells which are
specialised epidermal cells.
Setiap stoma diapit oleh dua sel pengawal, yang mengawal atur saiz stomata.
Each stoma is flanked by two guard cells, which regulate the size of the stomata
pore.
Stomata dan ruang udara antara sel membentuk sistem pengudaraan. Stomata
memainkan peranan penting dalam fotosintesis dengan membenarkan
pertukaran ………….. antara bahagian dalam daun dan persekitaran luaran.
Stomata and intercellular air spaces form an aeration system. Stomata play an
essential role in photosynthesis by allowing the exchange of ………… between
the interior of the leaf and the external environment.
Karbon dioksida dari atmosfera meresap ke dalam daun melalui stomata ketika
mereka terbuka sementara oksigen (hasil sampingan fotosintesis) meresap
keluar dari daun melalui jalan yang sama (stoma).
Carbon dioxide from the atmosphere diffuses into the leaf through the stomata
when they are open while oxygen (a by-product of photosynthesis) diffuses out
of the leaf through the same route.
Air tersejat keluar dari stomata melalui transpirasi.
Water evaporates out of the stomata through transpiration.
1. Berikan dua ciri daun yang membolehkannya disesuaikan untuk menjalankan fotosintesis.
Give two characteristics of a leaf that enable it to adapt for photosynthesis.
………………………………………………………………………………………………………..
………………………………………………………………………………………………………..
2. Bahagian daun yang manakah membantu tumbuhan mengurangkan kehilangan air dalam keadaan
normal?
Which parts of the leaf help the plant to reduce water loss under normal conditions?
………………………………………………………………………………………………………...
………………………………………………………………………………………………………..
5. Bagaimanakah transpirasi pada tumbuhan terjejas jika bahagian bawah permukaan daun disapukan
dengan jeli petroleum?
How transpiration in plants affected if the bottom of the leaf surfaces is smeared with petroleum
jelly?
……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
7. Nyatakan perbezaan struktur antara sel mesofil palisade dan sel mesofil berspan.
State the differences in the structure between palisade mesophyll cells and spongy mesophyll cells.
Sel mesofil palisad Sel mesofil berspan
Palisade mesophyll cells Spongy mesophyll cells
Struktur kloroplas
Structure of a chloroplast TP 1
Tilakoid
Thylakoid
Kantung berbentuk ………….
………… shape.
Mengandungi pigmen klorofil yang berfungsi untuk menyerap …………. di dalam membrannya.
Has chlorophyll pigments that absorb ……….. in its membrane.
Mengandungi system pengangkutan electron.
Has a system of electron transport.
Tapak untuk tindak balas bersandarkan cahaya.
Site for reactions that are dependent of light.
Stroma Granum
Stroma Granum
Bendalir tidak berwarna yang mengandungi Terbenam dalam …………
pelbagai enzim dan butiran …………... Embedded in ………..
Colourless fluid that contains different Struktur yang terdiri daripada beberapa
types of enzymes and …………. particles. tilakoid yang diatur membentuk lapisan
Tapak untuk tindak balas tidak It is made up a few thylakoids that are
bersandarkan cahaya. arranged in layers.
Location for reactions that are independent Mengandungi pigmen …………. dan
of light. enzim fotosintesis.
Has ………….. pigments and
photosynthetic enzymes.
Susunan lapisan ini menjadikan kawasan
permukaan adalah optimum untuk
fotosintesis.
Its stacking arrangement optimizes the
surface area for photosynthesis
Membrane luar Membrane dalam
Outer membrane Inner membrane
Memisahkan kloroplas daripada bahagian Mengawal keluar masuk bahan ke dalam
sel yang lain. kloroplas.
Separate chloroplast from other parts of the Control movement of materials in and out
cell. of chloroplast.
Kromatografi TP 2
Chromatography
Aktiviti 2.3
Activity 2.3
Tujuan Menjalankan penyiasatan untuk mengasingkan pigmen-pigmen fotosintesis di dalam
Aim daun dengan menggunakan kromatografi kertas
To carry out an investigation to separate photosynthetic pigments in a leaf using paper
chromatography
Bahan Daun pokok ati-ati/ daun pokok pandan/ daun bayam merah, pasir halus, aseton 80%,
Material pelarut (satu bahagian aseton dan Sembilan bahagian eter petroleum), lidi, kertas turas,
pensel
Coleus plant leaves/ pandan leaves/ red spinach leaves, fine sand, 80% acetone, solvent
(one part of aceton and nine parts of petroleum ether), skewer, filter paper, pencil
Radas Bekas kaca, pembaris, lesung dan alu
Apparatus Glass container, ruler, mortar and pestle
Prosedur 1. Tumbuk dua helai daun pokok ati-ati/ daun pandan/ daun bayam merah
Procedure bersama-sama aseton 80% dan sedikit pasir menggunakan lesung serta alu
sehingga ekstrak daun menjadi agak pekat.
Pound two leaves of coleus plant/ pandan leaves/ red spinach leaves with 80%
acetone and a little bit of fine sand using a mortar and pestle until a thick leaf
extract is obtained.
2. Sediakan sekeping kertas turas berukuran 3cm x 15cm.
Prepare a filter paper of size 3 cm × 15 cm.
3. Buat satu garisan dengan jarak 1.5cm dari hujung kertas turas se4cara
mendatar menggunakan pensel.
Draw a line of 1.5 cm from the end of the filter paper horizontally using a
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
pencil.
4. Pindahkan titisan ekstrak daun ke tengah garisan pensel dan keringkan.
Transfer the leaf extract in the middle of the pencil line and let it dry.
5. Ulangi langkah 4 sebanyak sepuluh kali unutk mendapatkan titik ekstrak yng
pekat pada kertas turas.
Repeat step 4 ten times to get a thick extract on the filter paper.
6. Celupkan hujung kertas turas ke dalam bekas kaca yang mengandungi pelarut
pada ketinggian 1cm.
Dip the end of filter paper in a glass container containing solvent at a height of
1 cm
7. Biarkan kertas turas kering seketika dan perhatikan perubahan.
Let the filter paper dry for a while and observe the changes.
8. Tandakan kedudukan setiap pigmen yang kelihatan pada kromatogram.
Mark the points of each pigment that can be seen on the chromatogram.
.
Perbincangan 1. Fungsi pelarut adalah untuk melarutkan …………...
The fuction of solvent are to dissolve ………………..
2. Pasir halus digunakan semasa menyediakan ekstrak daun untuk …………….
sel-sel daun untuk mengektrak klorofil dengan mudah.
Fine sand is used while preparing the leaf extract to ……………… the leaf
cells to extract chlorophyll easily
3. Pigmen-pigmen fotosintesis di dalam daun dapat diasingkan menggunakan
kaedah kromatografi berdasarkan nilai Rf iaitu nisabah jarah yang dilalui oleh
pigmen kepada jarak yang dilalui oleh pelarut sepanjang kromatogram.
The photosynthetic pigments in the leaves can be separated using
chromatographic method based on the value of Rf which is the ratio of the
distance traveled by the pigment to the distance traveled by the solvent along
the chromatogram.
Tindak balas bersandarkan cahaya dan Tindak balas tidak bersandarkan Cahaya
Light-Dependent and Light-Independent Reactions TP 1
Tindak balas keseluruhan fotosintesis boleh diwakili oleh persamaan kimia berikut:
The overall reaction of photosynthesis can be represented by the following chemical reaction:
Tenaga cahaya TP 2
Light energy
12H2O + 6CO2 C6H12O6 + 6O2 + 6H2O
Klorofil
Chlorophyll
air karbon dioksida glukosa oksigen air
water carbon dioxide glucose oxygen water
Persamaan dan perbezaan antara tindak balas bersandarkan cahaya dengan tindak balas tidak bersandarkan
cahaya
Similarities dan differences between the light-dependent and light-independent reactions TP 4
Keamatan cahaya
Light intensity
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Suhu
Temperature
Pada suhu yang lebih ………… daripada suhu
optimum, kadar fotosintesis menurun.
At temperatures ……….. than the optimum
temperature, the rate of photosynthesis decreases
30
40
50
60
15
25
35
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
45
55
65
75
0.02
0.03
0.04
0.05
0.06
0.07
0.08
0.09
0.10
Kesan perubahan keamatan cahaya dan warna cahaya terhadap kadar fotosintesis
TP 2
Effect of change in light intensity and light colours on rate of photosynthesis
2. Apakah warna cahaya yang paling sesuai untuk memaksimumkan kadar fotosintesis?
What colour of the light is most suitable for maximizing the rate of photosynthesis?
………………………………………………………………………………………………………….
1. Bagaimanakah negara yang mengalami perubahan empat musim dapat memperoleh dan
mengekalkan hasil pertanian?
How can a country undergoing a four -season change be able to obtain and maintain agricultural
produce?
………………………………………………………………………………………………………….
2. Apakah masalah-masalah yang dihadapi oleh Negara yang mengalami empat musim ini?
What are the problems faced by the Country experiencing these four seasons?
a) Keamatan cahaya dan ………. persekitaran yang tidak sekata sepanjang tahun.
Uneven light intensity and ambient …………… throughout the year.
b) Pada musim luruh, kebanyakan pokok menggugurkan ………….. dan ini menyebabkan kadar
fotosintesis menjadi rendah.
In autumn, most trees drop their …………. and this causes the rate of photosynthesis to be low.
c) Pada musism sejuk, tempoh waktu siang adalah singkat, keamatan cahaya ………….. dan suhu
persekitaran sangat rendah. Oleh itu, kadar fotosintesis menjadi sangat rendah.
In cold weather, the daylight hours are short, the light density is …………… and the ambient
temperature is very low. Therefore, the rate of photosynthesis becomes very low.
1. Titik pampasan ialah aras keamatan cahaya apabila kadar respirasi adalah ……….. dengan kadar TP 1
fotosintesis.
The compensation point is the level of light intensity when the rate of respiration is …………. to the
rate of photosynthesis.
2. Pada titik pampasan, kadar fotosintesis adalah sama dengan kadar respirasi.
At the point of compensation, the rate of photosynthesis is equal to the rate of respiration.
3. ………….. yang dihasilkan dalam fotosintesis digunakan dalam respirasi tumbuhan.
…………….. produced in photosynthesis is used in plant respiration.
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Pada keamatan cahaya rendah Apabila keamatan cahaya Pada keamatan cahaya tinggi
At low light intensity meningkat At high light intensity
When the light intensity
increases
Kadar respirasi ………….. kadar Pada keamatan cahaya tertentu, Kadar fotosintesis ………….
fotosintesis. kadar respirasi dan fotosintesis kadar respirasi.
The rate of respiration …………. adalah …………. The rate of photosynthesis
the rate of photosynthesis At a particular light intensity, ……………. the rate of
therates of respiration and respiration
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
photosynthesis are the
…………...
Pengambilan bersih Jumlah ……………… yang Pengambilan bersih karbon
………….. dan pembebasan dibebaskan oleh respirasi dioksida dan pembebasan
bersih karbon dioksida adalah sama dengan jumlah bersih …………….
A net uptake of …………. karbon dioksida yang A net uptake of carbon
and a net release of carbon digunakan dalam fotosintesis. dioxide and a net release of
dioxide The amount of …………..
……………….. produced by
respiration is equal to the
amount of carbon dioxide used
in photosynthesis
Tiada pengambilan atau
pembebasan bersih karbon
dioksida atau oksigen oleh
tumbuhan.
There is no net uptake or
release of carbon dioxide or
oxygen by the plant.
Ini dikenali sebagai titik
pampasan, dimana kadar
…………. karbon dioksida
adalah sama dengan kadar
pengambilan karbon dioksida.
This is called the
compensation point, where the
rate of carbon dioxide ………..
is equal to the rate of carbon
dioxide uptake.
Fotosintesis Respirasi
Photosynthesis Respiration
Persamaan
Similarities
Kedua-dua proses berlaku dalam organisma hidup
Both processes take place in living organisms
Kedua-dua proses melibatkan pengambilan dan pembebasan …………..
Both processes involve the uptake and release of …………..
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Perbezaan
Differences
Organisma yang terlibat ialah tumbuhan ………. Organisma yang terlibat ialah semua organisma
dan bakteria fotosintetik ……………
Organisms involved is ………… plants and Organisms involved is all …………… organisms
photosynthetic bacteria
Berlaku dalam sel yang mengandungi ………….. Berlaku dalam semua sel
Occurs in the cells containing ………….. Occiurs in all cells
Berlaku proses ………….., iaitu mensisntesis Berlaku proses …………., iaitu penguraian glukosa
glukosa menggunakan gas karbon dioksida dan air untuk menghasilkan tenaga
…………… process happens, which is the …………… process happens, which is the
synthesis of glucose using carbon dioxide and water breakdown of glucose to produce energy
Berlaku dalam ………… Berlaku dalam …………………
Occurs in ………….. Occurs in ………………..
Bahan tindak balas ialah gas ……………… dan air Bahan tindak balas ialah gas ………… dan glukosa
Reaction substances is ……………. and water Reaction substances is ………… and glucose
Hasil tindak balas ialag ……………. Hasil tindak balas ialah ………………..
Reaction products is ……………. Reaction product is ………………
Hasil sampingan ialah gas oksigen dan air Hasil sampingan ialah gas karbon dioksida dan air
By-products is oxygen and water By-products is carbon dioxide and water
Tenaga cahaya diserap dan ditukarkan kepada Tenaga kimia ditukarkan kepada ATP dan tenaga
tenaga kimia haba dibebaskan
Light energy is absorbed and converted into Chemical energy is converted to ATP and heat
chemical energy energy is released
Memerlukan ……………… Tidak memerlukan cahaya
Needs ……………. Does not need light
Menganalisis keamatan cahaya dan pencapaian titik pampasan dengan menggunakan graf
Analyse light intensity and attainment of compensation point using a graph TP 4
CHAPTER 2: LEAF STRUCTURE AND FUNCTION (12.5.2023) PG 26-57
Kesan kadar fotosintesis dan kadar respirasi sel yang kekal pada titik pampasan terhadap pertumbuhan dalam
tumbuhan
The effect on plant growth if the rate of photosynthesis and the rate of cellular respiration remain at its
compensation point