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Cyto Tzanck or Mucosal Scrapes

This document provides instructions for collecting cytology specimens for Tzanck tests or superficial skin/mucosal scrapings. It outlines the preferred collection vials, unacceptable collection methods, necessary materials, patient preparation, collection procedure, limitations, and references. The key steps are to scrape the lesion margin using a metal spatula or scalpel, rinse into a preservcyt or thinprep vial, label, complete a requisition form, and send to the lab without using the hospital tube system.

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Rahmahdini Emil
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95 views2 pages

Cyto Tzanck or Mucosal Scrapes

This document provides instructions for collecting cytology specimens for Tzanck tests or superficial skin/mucosal scrapings. It outlines the preferred collection vials, unacceptable collection methods, necessary materials, patient preparation, collection procedure, limitations, and references. The key steps are to scrape the lesion margin using a metal spatula or scalpel, rinse into a preservcyt or thinprep vial, label, complete a requisition form, and send to the lab without using the hospital tube system.

Uploaded by

Rahmahdini Emil
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Mississippi Baptist Medical Center Department of Pathology 1225 North State Street Jackson, MS 39202

Document No: 135 Version No: 1 Effective Date: 06/27/2007 Cytology

Cytology Specimen Collection--Tzanck Test Or Superficial Skin/Mucosal Scrapings


Purpose
To establish collection methods for the Tzanck test and superficial skin/mucosal scrapings. Also, to ensure the safety of health care personnel by requiring careful handling of specimens containing blood or body fluids.

Principle
Collection methods must ensure maximum cell preservation and recovery for optimal cytologic evaluation. All specimens must be submitted in well-constructed containers with secure-fitting lids to prevent leakage during transport and to protect health care personnel from exposure. Certain viral infections (e.g. Herpes) produce characteristic features readily recognized on cytologic preparations.

Preferred Specimen
Scrapings obtained must be submitted in a Preservcyt (green and white label) or ThinPrep Pap collection vial.

Unacceptable Specimen
Conventional smears are not accepted. Cotton-tipped swabs are not acceptable collection devices.

Materials Needed
1. A Preservcyt (green and white label) or ThinPrep Pap collection vial (obtainable from cytology or Laboratory Outreach Services) 2. A disposable needle 3. A metal spatula or scalpel blade for the collection device. DO NOT use a cottontipped swab. 4. A Non-Gyn Cytology Consultation Form (obtainable from cytology or Laboratory Outreach Services)

Patient Preparation
1. Moisten the lesion with saline. If possible, a fresh vesicle should be chosen that has not ruptured and crusted. 2. With a disposable needle, open a fresh vesicle or remove the crust from a ruptured lesion.

Procedure
1. Using the edge of a metal spatula or scalpel blade, scrape the margin of the lesion. The edges of the lesion will have the best yield of cells with morphologically recognizable inclusions
For use by MBHS. Printed copies are for reference only. Refer to electronic version to view approval and staff review documentation.

Cytology Specimen Collection--Tzanck Test Or Superficial Skin/Mucosal Scrapings Document No: 135 Version No: 1

2. Rinse the material into a Preservcyt or ThinPrep collection vial, vigorously swirling the spatula ten times. 3. Place the cap on the vial and tighten it so that the torque line on the cap passes the torque line on the vial. 4. Label the vial with two forms of the patients unique identification. 5. Complete a Non-Gyn cytology consultation/requisition form. Refer to Cytology Consultation Form Completion--Non-Gyn for required elements. 6. Indicate on the form the source of the specimen. 7. Send the specimen and Non-Gyn form to the lab. Do not use the hospital tube system to transport any cytology specimen. 8. The specimen does not require refrigeration.

Limitations
1. Collecting poorly preserved cells does not provide an optimal specimen for cytologic evaluation. 2. Ulcerated lesions may produce a necrotic inflammatory background mimicking tumor diathesis. 3. Reactive/reparative changes may result in atypical pleomorphic cells mimicking cancer.

References
1. The Art and Science of Cytopathology, 1996, DeMay, R., ASCP Press, p. 337. 2. Nongynecologic Cytologic Specimens: Collection and Cytopreparatory Techniques; Approved Guideline, NCCLS document GP23-A, Vol 19 No. 14, Aug 1999, p. 7.

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