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Sigma Wide Marker

SigmaMarker(tm) high, low, and wide range protein standards are specially designed for use in the PhastSystem electrophoresis workstation and in standard Laemmli SDS-PAGE systems. The High Range contains seven proteins from 36-200 kDa; the low range contains eight proteins from 6.5-66 kda; the wide range contains twelve.

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Marek Adamczak
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259 views2 pages

Sigma Wide Marker

SigmaMarker(tm) high, low, and wide range protein standards are specially designed for use in the PhastSystem electrophoresis workstation and in standard Laemmli SDS-PAGE systems. The High Range contains seven proteins from 36-200 kDa; the low range contains eight proteins from 6.5-66 kda; the wide range contains twelve.

Uploaded by

Marek Adamczak
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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SigmaMarker High Range, Molecular Weight 36,000200,000 Da Catalog Number S8320 Low Range, Molecular Weight 6,50066,000 Da Catalog

Number M3913 Wide Range, Molecular Weight 6,500200,000 Da Catalog Number S8445 Storage Temperature 28 C

TECHNICAL BULLETIN
Product Description High, Low, and Wide Range SigmaMarker protein standards are specially designed for use in the PhastSystem electrophoresis workstation and in standard Laemmli SDS-PAGE systems. The High Range SigmaMarker contains seven proteins from 36200 kDa. The Low Range SigmaMarker contains eight proteins from 6.566 kDa. The Wide Range SigmaMarker contains twelve proteins from 6.5200 kDa (see Table 2). When using the Wide Range SigmaMarker, gradient gels are recommended. Reconstitution with 100 L of deionized water results in a solution containing 23.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.005% bromophenol blue. Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Preparation Instructions 1. To prepare the SigmaMarker for application to gels that will be stained with Brilliant Blue, reconstitute each vial with 100 L of deionized water. Vortex for a few seconds to ensure the material is completely dissolved. Immediately aliquot and freeze the unused portions. Note: Heating of the reconstituted marker is not recommended. 2. To prepare the SigmaMarker for application to gels that will be silver stained, dilute the marker reconstituted for gels to be stained with Brilliant Blue (step 1) 50-fold with 20% glycerol solution. Storage/Stability Store the lyophilized powder desiccated at 28 C. After reconstitution, store below 20 C. Repeated freezing and thawing of reconstituted SigmaMarkers is not recommended. Procedure Select an appropriate marker loading volume specific for the type of gel system to be used (see Table 1). Note: For complete separation of the low molecular weight protein standards, it is recommended to use a 420% gradient gel or a homogeneous gel of greater than 15%. Table 1. Recommended Marker Volumes for Various Gel Systems Recommended Gel System Marker Volume PhastGel Medium SDS-Laemmli Mini gel (10 8 cm) SDS-Laemmli Large gel (18 16 cm) 1 L 35 L 510 L

References 1. Laemmli, U.K., Nature, 227, 680 (1970). SigmaMarker is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. PhastSystem is a trademark of GE Healthcare PhastGel is a registered trademark of GE Healthcare

Table 2. Molecular Weights Protein Myosin from porcine heart -Galactosidase from E. coli Phosphorylase b from rabbit muscle Albumin, bovine serum Glutamic Dehydrogenase from bovine liver Ovalbumin from chicken egg Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle Carbonic Anhydrase from bovine erythrocytes Trypsinogen from bovine pancreas Trypsin Inhibitor from soybean -Lactalbumin from bovine milk Aprotinin from bovine lung Molecular Weight (Da) 200,000 116,000 97,000 66,000 55,000 45,000 36,000 29,000 24,000 20,000 14,200 6,500 High Range (S8320) X X X X X X X Wide Range (S8445) X X X X X X X X X X X X X X X X X X X X Low Range (M3913)

AF,KEK,PHCMAM 11/09-1

Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conformproducts are sold through Sigma-Aldrich, Inc. Sigma-Aldrich publications. Purchaser Sigma brand to the information contained in this and other must determine the suitability of the product(s) for their particularthe information contained inconditions maySigma-Aldrich publications. side of Sigma-Aldrich, Inc. warrants that its products conform to use. Additional terms and this and other apply. Please see reverse Purchaser must determine the suitability of the product(s) foror packing slip. use. Additional terms and conditions may apply. the invoice their particular Please see reverse side of the invoice or packing slip.

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