User Manual

Series 8000DH
CO2 Incubator
Direct Heat with Sterilization Cycle
Operating and Maintenance Manuals 7013540 Rev. 4

Visit us online to register your warranty

www.thermoscientific.com/labwarranty
 Preface

Model Sensor* Voltage**

3540 T/C 115
3541 T/C 230
3542 IR 115
3543 IR 230

*T/C is a thermal conductivity sensor.

IR is an infra-red sensor.
**All units are 50/60 Hz.

MANUAL NUMBER 7013540

4 31343/IN-4592 10/22/14 New magnetic door switch (parts list, expl dwg, elec schematics) RoHS ccs
3 29174/IN-4469 3/5/14 Updated solenoid - pgs 8-5 & 9-1 through 9-3 ccs
2 30384/IN-4435 1/7/14 Added SRO ext door part number 1900567 to parts list ccs
1 24851, 24849/IN-3971 1/7/10 CO2 valve connection inlet & inner door switch kits-parts list & drawing 370-200-5 ccs
0 25694/IN-3954 10/26/09 Release level 2 - added spring rod kit (pg. 1-7) ccs
REV ECR/ECN DATE DESCRIPTION By

Thermo Scientific Direct Heat CO2 Incubator i

Preface

CAUTION
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.

Warning If the incubator is not used in the manner specified in this operating manual, the protection provided
by the equipment design may be impaired. s

©2007 Thermo Scientific. All rights reserved.

ii Direct Heat CO2 Incubator Thermo Scientific

 Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermofisher.com.

4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientific Direct Heat CO2 Incubator iii

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

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Our Sales Support staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.

Our Service Support staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.

When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific

401 Millcreek Road, Box 649
Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

iv Direct Heat CO2 Incubator Thermo Scientific

 Preface

Warranty Notes
Information You Should Know Before Requesting Warranty Service
• Locate the model and serial numbers. A serial tag is located on the unit itself.
• For equipment service or maintenance, or with technical or special application inquiries, contact your local
distributor.

Repairs NOT Covered Under Warranty

• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
a billable service at your location. Calibration after a warranty repair is covered under the warranty.
• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
• Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient temperature fluctuations
8) any repair external to the unit
• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
• Repairs to parts or systems resulting from unauthorized unit modifications.
• Any labor costs other than that specified during the parts and labor warranty period, which may
include additional warranty on CO2 sensors, blower motors, water jackets, etc.

Thermo Scientific Direct Heat CO2 Incubator v

 Table of Contents
Section 1 Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Stacking the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Duct Sheets and Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Access Port Filter & CO2 Sensor Cover Plate . . . . . . . . . . . . . . . . .1-9
Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
HEPA Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Electrical Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Filling the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Connecting the CO2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . .1-12
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-13

Section 2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Thermal Conductivity CO2 System . . . . . . . . . . . . . . . . . . . . . . . .2-2
Infrared CO2 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Relative Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4

Thermo Scientific Direct Heat CO2 Incubator vi

Table of Contents

Section 3 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Turn Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set REPLACE HEPA filter reminder . . . . . . . . . . . . . . . . . . . . . . .3-2
Set Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Enable Low Temp Alarm to Trip Contacts . . . . . . . . . . . . . . . . . . .3-3
Set Low Temp Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-3
Set High CO2 Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-4
Set Low CO2 Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-4
Set New Zero Number for T/C CO2 Sensors . . . . . . . . . . . . . . . .3-5
Enable CO2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .3-5
Setting a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Set New Span Number for T/C CO2 Sensors . . . . . . . . . . . . . . . .3-6
Enabling Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . .3-7
Enable RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-7

Section 4 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Temp Controller Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
TMP CNTR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Section 5 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Clean Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Replace Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Clean Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Clean Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Replace Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Electronics Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Sterilization Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Sterilization Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8

vii Direct Heat CO2 Incubator Thermo Scientific

 Table of Contents

Section 6 Factory Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Remote Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Humidity Readout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Section 7 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Section 8 Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Exploded Part Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2

Section 9 Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Section 10 Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Thermo Scientific Direct Heat CO2 Incubator viii

 Section 1 Installation and Start-Up

Chamber Gas
Sample Port Control Panel

Power Steri

Cycle
Sterilization
Switch Cycle Button

See
Section 1
Inner Door

Leveling
Legs (4)
Door Heater See Section 5
Cable
Figure 1-1. Components

• Chamber Gas Sample Port - Used for sampling chamber CO2 content
using a FYRITE or similar instrument.

• Main Power Switch - Mains Disconnect

• Control Panel - Keypad, displays & indicators (Figure 1-2)

• Leveling Legs - Used to level the unit

• Sterilization Cycle Button - Switch to initiate sterilization cycle

Note The incubators are stackable. Information follows. s

Thermo Scientific Direct Heat CO2 Incubator 1-1

Section 1
Installation and Start-Up

- Mutes the audible alarm.

Alarm Indicator - Light pulses on/off during an alarm condition.
MODE Select Switch - Used to select Run, Setpoints, Calibration and
Configuration modes.
Message Display - Shows system status.
Mode Select Indicators -
RUN: Run Menu
SET: Set Points Menu
CAL: Calibration Menu
CON: Configuration Menu
HEAT Indicator - Lights when heaters are activated.
INJ Indicator - Lights during CO2 injection into chamber.
T (°C) Display - Programmable to display temperature. See
Configuration section.
RH (%) Display - Shows percentage of humidity. See Configuration sec-
tion.
CO2 (%) Display - Programmable to display CO2 percentage. See
Configuration section.
Arrow Keys (Scroll for Parameters) - Increases or decreases number val-
ues, toggles between mode choices.
ENTER - Stores the selected value into unit memory.

Silence Alarm Mode Selector Active Heat Indicator Temperature ENTER

Indicator Switch Mode Select Indicators in °C
Switch button

Message Display

Power Switch Active Injection Indicator Scroll Arrow Keys

Humidity in %

CO2 level in %
Figure 1-2. Control Panel

1-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation and Start-Up

Keypad Operation The Series 8000DH incubator has four basic modes for incubator setup:
Run, Setpoints, Calibration and Configuration.

• Run is the default mode for normal operation.

• Set is used to enter system setpoints for incubator operation.

• Calibration is used to calibrate various system parameters.

• Configuration allows for custom setup of various options.

Table 1-1. Selections Under Each Mode

RUN SETPOINT CALIBRATION CONFIGURATION

Default Mode Temperature Temp Offset Audible
1
Overtemp CO2 Cal New HEPA Timer
2
CO2 IR Cal Replace HEPA Reminder
RH Cal Access Code
Temp Lo Alarm
Temp Relay
CO2 Lo Alarm
CO2 Hi Alarm
CO2 Relay
CO2 Z & S #'s *
RH Lo Alarm
RH Relay
Display Temp
Display RH
1
T/C units Base Unit Displays
2
IR units Option Unit Displays
*T/C units only

Thermo Scientific Direct Heat CO2 Incubator 1-3

Section 1
Installation and Start-Up

Keypad Operation SCROLL FOR PARAMETERS Arrows: Steps the operator through
parameters of SET, CAL and CON modes. The right arrow goes to the
(continued) next parameter, the left arrow returns to the previous parameter.
Up/Down Arrows: Increases/decreases or toggles the parameter value
selected in SET, CAL, and CON modes.
ENTER: Must press this key to save-to-memory all changed values.
Key: Press to mute audible alarm. See Section 4 for alarm ringback
times.

Displays Message Display: Shows system status (Mode) at all times. Shows ‘CLASS
100’ or ‘SYSTEM OK’ during normal operation, or alarm messages if
the system detects an alarm condition (see Section 4, Alarms). The mes-
sage ‘CLASS 100’ is a timing mechanism indicating that, under normal
operating conditions with the HEPA filter installed, the air inside the
chamber meets the Class 100 air cleanliness standard for particulates of
0.5 micron size or larger per cubic foot of air. Further information on
the Class 100 classification of air quality is available from Thermo.
Upper and Lower Displays: The 7 segment upper display shows temp and
RH. The lower display shows CO2 and O2.

Installing the 1. Maintain a minimum three inch clearance behind the incubator for
electrical and gas hook-ups. In addition, a three inch ventilation space
Incubator is needed on each side.

2. Locate the unit on a firm level surface capable of supporting the unit
weight of 260 lbs (118kg).

3. Locate the unit away from doors and windows and heating and air
conditioning ducts.

4. Lift the unit by the sides of the cabinet base. Do not attempt to lift it
by the front and back. This places stress on the outer door hinges.

1-4 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation and Start-Up

Stacking the Warning Install stacked units against a wall or similar structure. s
Incubators
Warning With incubators in a stacked configuration, do not leave both
exterior doors open at the same time. s

Warning If the units have been in operation, turn them both off and
disconnect the power source before beginning any service work. s

Two stacking brackets (shown at left) are included in the

parts bag shipped with each incubator.

Figure 1-3. Stacking Brackets

1. Remove the cover plate securing the door cord from the incubator to
be on top. See Figure 1-4. Disconnect the plug from the connector.

2. Remove the four screws securing the door hinges to the unit. Remove
the door and set it aside.

Cover plate and bolt hole

with door connector

Figure 1-4. Cover Plate Figure 1-5. Hole Plug Locations

3. Unscrew the two hole plugs from the top cover of the incubator to be
the bottom of the stack (Figure 1-5).

4. Unscrew and remove the 4 leveling feet from

the unit to be stacked on top and lift it onto
the bottom unit. Align all sides.

Warning This incubator weighs 260 lbs (118kg).

Have sufficient personnel available when lifting.
Lift the unit by the sides of the cabinet base to
avoid placing stress on the outer door hinge. s

Figure 1-6. Stacked

Thermo Scientific Direct Heat CO2 Incubator 1-5

Section 1
Installation and Start-Up

Stacking the 5. Insert the stacking brackets into the slots at the back of the stacked
units as shown in Figure 1-7.
Incubators (cont.)
6. Align the slotted holes in the brackets with the mounting holes on the
back of the top incubator. Secure the brackets with the screws and
washers provided in the parts bag. See Figure 1-8.

Top incubator back

Top Stacking
un Stacking
it bracket bracket
Bo
tto
m
un Bottom incubator back
it

Figure 1-7. Bracket Figure 1-8. Installed Brackets on Back of Unit

into Slot
7. Thread one 1/4 x 20 bolt and washer
included with the stacking brackets, Cover plate and bolt hole
into the hole behind the cover plate. with door connector
Do not tighten. Refer to Figure 1-9.
8. Remove the cover plate from the same
area on the other side of the top unit. Figure 1-9. Bolt Hole
9. Thread the other 1/4 x 20 bolt and
washer into this hole.
10. Tighten the bolts on both sides.
11. Assemble the door hinges to the unit. Secure with the screws.
12. Plug the door cord into the connector, as previously. Secure the cover
plate.
13. Install the cover plate on the other side of the unit. The stacked
incubators are ready for service.

Preliminary Cleaning 1. Remove vinyl from shelf channels, duct sheets, and air duct, if present.
2. Using a suitable laboratory disinfectant, clean all interior surfaces.

Caution Before using any cleaning or decontamination method except

those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user. s

1-6 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation and Start-Up

Duct Sheet and Shelf 1. Installed included grommets inot the back
flange of each duct sheet; 6 grommets per
Installation sheet. See Figure 1-10.

2. Install the side ducts with the tabs facing into

the center of the chamber with their slots up.
There are no right side or left side ducts,
simply rotate one of them to fit the opposite
side. Tilt the side ducts as they are placed into Figure 1-10. Grommets
the chamber so the tops fit into the top air on back of duct sheet
duct, then guide them into the vertical position.
Figure 1-11 shows the side duct as it would be
oriented for the right side of the chamber.

Side toward side duct

Shelf Channels

Side toward shelf

Shelf channel Side duct Shelf channel

rear slot tab front slot
Side duct
tab
Side Duct
Right Side Duct
with Shelf Channel Installed
Figure 1-11. Shelf Channels & Side Ducts Figure 1-12. Channels and Slots

3. Referring to Figure 1-11, note that there is no difference in the left

and right shelf channels.

4. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the side duct. Pull the shelf channel forward
and engage the channel’s front slot into the side duct’s appropriate
forward tab. Refer to Figure 1-12.

5. Figure 1-11 shows one of the channels installed on the right side duct.

Thermo Scientific Direct Heat CO2 Incubator 1-7

Section 1
Installation and Start-Up

Installing Duct Sheets 6. Locate the supplied rod, spring and end pieces; 4 each. Assemble the
spring to the rod by positioning the very end of the spring over the
and Shelves (cont.) ridge at the rod. Then press the spring to the opposite side. It should
snap into place. See Figures 1-13 and 1-14.

Ridge
Spring
end

Figure 1-13. Spring End Figure 1-14. Spring Assemble to Rod

7. Slide end cap over spring

(Figure 1-15).

Note When installing the rods, the

spring end can be installed on
either side of the chamber. The
upper front rod is high in the
chamber and the upper back rod is Figure 1-15. Spring End
lower to allow access to the access
port filter door. s

8. Install one end of the rod into the appropriate hole in the duct sheet.

9. Compress the spring (under the end cap) to insert the other end of the
rod into the hole in the duct sheet on the opposite side.

10. Figure 1-16 shows the four Front opening of chamber

rods installed.

Back wall of chamber

Figure 1-16. Four Rods Installed in Chamber

1-8 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation ans Start-Up

Access Port Filter & 1. Locate the opening in the top left corner of the interior chamber.
Remove the tape from the opening on the outside of the unit.
CO2 Sensor Cover Plate
2. Locate the stopper with filter in the hardware bag. Lift the metal port
cover and install the assembly in the opening inside the chamber. See
Figure 1-17.

3. Also in the hardware bag is the CO2 sensor cover plate. Install, using
the 1/4-turn fasteners. Refer to Figure 1-17 for the location.

Figure 1-17. Filter and Plate

Air Sample Filter 1. Remove the filter from the shipping bag.
Installation 2. Install the air sample filter assembly to the black hose barb behind the
top duct.

3. Insert the other end of the filter assembly onto the metal tubing on the
top duct. Refer to the Figure 1-18.

Figure 1-18. Air Sample Filter Installation

Thermo Scientific Direct Heat CO2 Incubator 1-9

Section 1
Installation and Start-Up

HEPA Filter Caution Be careful when the filter. The media can be damaged if it is
Installation mishandled. To avoid damage to the incubator, do not operate the unit
without the HEPA filter in place. s

1. Remove the filter from the shipping box.

2. Remove the plastic coating from the filter, using caution not to touch
the filter media.

3. Install the filter as shown in Figure 1-18. Refer to Section 5 for HEPA
filter maintenance.

Caution The media of the filter can be damaged if mishandled.

To avoid damage to the incubator, do not operate the unit without the
HEPA filter in place. s

Leveling the Unit Check the unit for being level by placing a bubble-style level on one of the
shelves. Turn the hex nut on the leveler counterclockwise to lengthen the
leg or clockwise to shorten it. Level the unit front-to-back and left-to-right.

Electrical Power See the serial tag on the side of the unit for electrical specifications or refer
Connection to the electrical schematics at the end of this manual.

Caution Serial tag rating is based on amperage draw during sterilization

cycle. Normal operating amperage is much less. Ensure that electrical
circuit will handle amp draw of sterilization cycle.
Connect the incubator to a grounded, dedicated circuit. The power cord
connector is the mains disconnect device for the incubator. Position the
unit so that it can be easily disconnected. s

1-10 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation and Start-Up

Electrical Power Plug the provided power cord into the power inlet connector on the back
of the cabinet (Figure 1-19), then into the grounded, dedicated, electrical
Connection (cont.) circuit.

Accessory
Outlet
75 watts max

ACCESSORY
75 WATTS MAX.

45 "&# #2
CO inject
#1
CO inject
15 p.s.i. Regulated 15 p.s.i. Regulated
(103.4 kPa) (103.4 kPa)

ALARMCONTACTS
30V MAX / 1A MAX

a
Remote b Power Cord
CO2 Inlet Air
Alarm Contacts Connector
#1 Tank Intake
30V max/1A max
a
RJ-ll telephone style connectors
b
IR CO2 units only

Figure 1-19. Back of Cabinet

Filling the Humidity Fill the humidity pan with sterile distilled water to within 1/2” of the top.
Place the pan directly in the center of the incubator floor.
Pan
For applications requiring high humidity, the pan should be placed against
the left side wall of the incubator. The side ducts have been modified to
allow the pan to be placed against the wall. Optimum humidity is achieved
by capping the CO2 sample port. This will, however, cause condensation
in the chamber. To enhance RH recovery from door openings, place a
second humidity pan in the right side duct.

For best operation of the incubator, sterilized distilled, demineralized or

de-ionized water should be used in the humidity pan. Water purity should
be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range
of 20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88
for measuring water purity.

Distillation systems, as well as some types of reverse osmosis water purity

systems, can produce water in the quality range specified. Tap water is not
recommended as it may contain chlorine, which can deteriorate the
stainless steel. Tap water may also have a high mineral content, which
would produce a build-up of scale in the pan. High purity or ultra pure
water is not recommended as it is an extremely aggressive solvent and will
deteriorate the stainless steel.

Thermo Scientific Direct Heat CO2 Incubator 1-11

Section 1
Installation and Start-Up

Filling the Humidity High purity water has a resistance of above 1M to 18M Ohm. Even high
Pan (continued) purity water can contain bacteria and organic contaminants. Water should
always be sterilized or treated with a decontaminant, safe for use with
stainless steel as well as safe for the product, prior to being introduced into
the humidity pan.

Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will
have an adverse effect on the humidity level and CO2 calibration of the
T/C units.

Caution Use of chlorinated water, or decontamination products containing

chlorine, will deteriorate the stainless steel and cause rust, voiding the
warranty. s

Connecting the CO2 Warning High concentrations of CO2 gas can cause asphyxiation! OSHA
Gas Supply Standards specify that employee exposure to carbon dioxide in any eight-
hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure
limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. s

Warning This incubator is designed to be operated with CO2 gas only.

Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO2 should not be connected to this equipment. CO2
gas cylinders have a UN1013 label on the cylinder and are equipped with
a CGA 320 outlet valve. Check the gas cylinder for the proper
identification labels. The CO2 gas supply being connected to the incubator
should be industrial grade, 99.5% pure. Do not use CO2 gas cylinders
equipped with siphon tubes. A siphon tube is used to extract liquid CO2
from the cylinder which can damage the pressure regulator. Consult with
your gas supplier to ensure that the CO2 cylinder does not contain a
siphon tube. Gas cylinders should also be secured to a wall or other
stationary object to prevent them from tipping.
A two-stage CO2 pressure regulator is required to be installed on the outlet
valve of the gas cylinder. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa) for proper performance of the CO2
control system. A single stage CO2 pressure regulator will not maintain 15
psig (103.4 kPa) to the incubator as the pressure in the CO2 cylinder
decreases; therefore, a two stage regulator is recommended.

1-12 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation and Start-Up

Connecting CO2 Gas Warning If higher purity CO2 is desired inside the incubator (greater than
99.5% pure), the pressure regulator should be constructed with a stainless
Supply (continued) steel diaphragm, along with specifying the purity of the CO2 from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder.
Consult your facility safety officer to ensure that the equipment is installed
in accordance with the codes and regulations that are applicable in your
area. s

The CO2 gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range. The low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa).

The incubator has serrated fittings on the back of the cabinet to connect
the gas supply. Refer to Figure 1-19. The fitting is labeled CO2 Inlet #1
Tank. Make sure that the connections are secured with clamps. Check all
fittings for leaks.

Incubator Start-Up With the incubator properly installed and connected to power, the
humidity pan filled, and the unit connected to gas supplies, system
setpoints can be entered. The following setpoints can be entered in SET
mode: Temperature, Overtemperature and CO2. To enter SET mode,
press the MODE key until the SET indicator lights. Press the right
and/or left arrow keys until the proper parameter appears in the message
display. See Chart 1-1 for more detail.

Setting the Operating All Series 8000DH incubators have an operating temperature range of
Temperature 10°C to 50°C, depending on ambient temperature. The incubator is
shipped from the factory with a temperature setpoint of 10°C. At this
setting, all heaters are turned off.

Thermo Scientific Direct Heat CO2 Incubator 1-13

Section 1
Installation and Start-Up

Setting the Operating To change the operating temperature setpoint:

Temperature (continued)
1. Press the MODE key until the SET indicator lights.

2. Press the right arrow until “Temp XX.X” is displayed in the message
display.

3. Press the up/down arrow key until the desired temperature setpoint is
displayed.

4. Press ENTER to save the setpoint.

5. Press the MODE key until the RUN indicator lights for RUN mode
or press the right/left arrow keys to go to next/previous parameter.

Setting the Overtemp Caution The independent overtemp system is designed as a safety to
Setpoint protect the incubator only. It is not intended to protect or limit the
maximum temperature of the cell cultures or customer’s equipment inside
the incubator if an overtemp condition occurs. s

Series 8000DH incubators are equipped with a secondary temperature

monitoring system to monitor the air temperature inside the cabinet. This
system is designed as a safety device to turn off all heaters in the event of a
temperature control failure. Temperature control in the incubator will be
±1° of the overtemp setpoint.

The overtemperature is set by the factory (default) at 40°C. However, the

overtemp can be set up to 55°C in 0.5° increments.

If the incubator’s operating temperature setpoint is set above the overtemp

setpoint, the overtemp setpoint will automatically update to 1°C above the
temperature setpoint. It is recommended that the overtemp setpoint be
maintained at 1°C over the operating temperature setpoint.

1-14 Direct Heat CO2 Incubator Thermo Scientific

 Section 1
Installation and Start-Up

Setting the Overtemp To set the Overtemp setpoint:

Setpoint (continued)
1. Press the MODE key until the SET indicator lights

2. Press the right arrow until Otemp XX.X is displayed in the message
display

3. Press the up or down arrow key until the desired Overtemp setpoint is
displayed

4. Press ENTER to save the setting

5. Press the MODE key until the RUN indicator lights or press the right
or left arrow to go to the next or previous parameter.

Setting the CO2 Setpoint All T/C CO2 cells are calibrated at the factory at 37°C, high humidity,
and 10% CO2. Therefore, if a temperature setpoint of 37°C has been
entered, the humidity pan has been filled and the CO2 control is to run
between 0-10% with a T/C CO2 sensor, the CO2 setpoint may be entered
immediately. Otherwise, it is important to allow the unit 12 hours to
stabilize at the temperature setpoint before entering the CO2 setpoint.

Both models of the incubator have a CO2 setpoint range of 0.0% to

20.0%. The incubator is shipped from the factory with a CO2 setpoint of
0.0%. At this setting, all CO2 control and alarms are turned off. To
change the CO2 setpoint:

1. Press the MODE key until the SET indicator lights.

2. Press the right arrow until “CO2 XX.X” is displayed in the message
display.

3. Press the up/down arrows until the desired CO2 setpoint is displayed.

4. Press ENTER to save the setpoint.

5. Press the MODE key until the RUN indicator lights to go to RUN
mode or press the right/left arrow keys to go to next/previous
parameter.

Thermo Scientific Direct Heat CO2 Incubator 1-15

Section 1
Installation and Start-Up

1-16 Direct Heat CO2 Incubator Thermo Scientific

 Section 2 Calibration
After the unit has stabilized, several different systems can be calibrated. In
Calibration mode, the air temperature, CO2 and RH levels can be
calibrated to reference instruments. To access Calibration mode, press the
MODE key until the CAL indicator lights. Press the right and/or left
arrow until the appropriate parameter appears in the message display. See
Chart 2-1 at the end of this section for more detail.

Calibration frequency is dependent on use, ambient conditions and

accuracy required. A good laboratory practice would require at least an
annual calibration check. On new installations, all parameters should be
checked after the stabilization period.

Prior to calibration, the user should be aware of the following system

functions. While the unit is in Calibration mode, all system control
functions are stopped so the unit remains stable. Readout of the system
being calibrated appears on the message display. If no keys are pressed for
approximately five minutes while in Calibration mode, the system resets to
RUN mode so control functions are reactivated.

Caution Before making any calibration or adjustments to the unit, it is

imperative that all reference instruments be properly calibrated. s

Temperature Before calibration, allow the cabinet temperature to stabilize. Place the
calibrated instrument in the center of the chamber. The instrument
should be in the airflow, not against the shelf.

Temperature Stabilization Periods

Startup - Allow 12 hours for the temperature in the cabinet to stabilize

before proceeding.

Already Operating - Allow at least 2 hours after the display reaches

setpoint for temperature to stabilize before proceeding.

Thermo Scientific Direct Heat CO2 Incubator 2-1

Section 2
Calibration

Temperature 1. Press the MODE key until CAL indicator lights.

(continued) 2. Press the right arrow until “TEMPCAL XX.X” appears in the message
display.

3. Press up/down arrow to match display to calibrated instrument.

4. Press ENTER to store calibration.

5. Press the MODE key to return to RUN or the right/left arrow to go to

next/previous parameter.

Thermal Conductivity Models 3540 and 3541 have a thermal conductivity (T/C) CO2 sensor.
CO2 System Thermal conductivity of the incubator atmosphere is not only effected by
the quantity of CO2 present but also by the air temperature and water
vapor present in the incubator atmosphere. In monitoring the effects of
CO2, air temperature and absolute humidity must be held constant so any
change in thermal conductivity is caused by a change in CO2
concentration.

Changing temperature or changing from elevated humidity to room

ambient humidity levels will necessitate a re-calibration of the CO2
control.

Some T/C CO2 sensors go through an aging period, especially on new

installations. Calibration should be checked on a weekly basis, and
adjusted as necessary. When stabilization occurs, checks can become less
frequent.

T/C CO2 Sensor Stabilization Periods

Start -Up - The CO2 sensor has been calibrated at the factory for 37°C
and elevated humidity. Allow the temperature, humidity, and CO2 levels
in the chamber to stabilize at least 12 hours before checking the CO2
concentration with an independent instrument.

Presently Operating - Make sure the chamber doors are closed. Allow at
least 2 hours after the temperature and CO2 displays reach their setpoints
for chamber atmosphere stabilization.

2-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 2
Calibration

Thermal Conductivity 1. Make sure the stabilization periods outlined above are followed.
CO2 System (continued) 2. Sample the chamber atmosphere through the sample port with an
independent instrument. Sample the atmosphere at least 3 times to
ensure accuracy of the instrument.

3. Press the MODE key until the CAL indicator lights.

4. Press the right arrow until “CO2 CAL XX.X” is displayed in the
message display.

5. Press the up /down arrows to change the display to match the

independent instrument.

6. Press ENTER to store the calibration.

7. Press the MODE key to return to RUN or the right or left arrows to
go to the next/ previous parameter.

Infrared CO2 System Models 3542 and 3543 have an infrared (IR) CO2 sensor. Infrared CO2
sensors are not effected by chamber atmosphere temperature or humidity.
However, the light detector in the sensor is effected by wide temperature
changes. Therefore, changing temperature setpoints could necessitate a
recalibration of the CO2. Chamber temperature should be allowed to
stabilize before checking CO2 concentrations with an independent
instrument, especially on start-up.

IR CO2 Sensor Stabilization Times

Start-Up- Allow the temperature and the CO2 of the cabinet to stabilize at
least 12 hours before proceeding.

Presently Operating - Allow CO2 to stabilize at least 2 hours at setpoint

before proceeding.

Thermo Scientific Direct Heat CO2 Incubator 2-3

Section 2
Calibration

Infrared CO2 System 1. Measure the CO2 concentration in the chamber through the gas
sample port with a Fyrite or other independent instrument. Several
(continued) readings should be taken to ensure accuracy.

2. Press the MODE key until the CAL indicator lights.

3. Press the right arrow until “IR CAL XX.X” appears in the message
display.

4. Press the up/down arrow to adjust the display to match the

independent instrument reading.

5. Press ENTER to store the calibration.

6. Press the MODE key to return to RUN mode.

Relative Humidity Model 3540 and 3541 incubators are equipped with an optional direct-
readout relative humidity sensor. This is a readout only of the chamber
relative humidity level. It does not provide any control of the relative
humidity in the cabinet.

Relative Humidity Stabilization Times

Start-Up - Allow 12 hours for the relative humidity and temperature in the
chamber to stabilize before proceeding.

Already Operating - Allow at least 2 hours after temperature display

reaches setpoint for relative humidity to stabilize before proceeding.

1. Place an accurate independent instrument in the center of the

chamber. Allow at least 30 minutes for RH to stabilize.

2. Press the MODE key until the CAL indicator lights.

3. Press the right arrow key until “RH CAL XX” appears in the message
display.

4. Press the up/down arrow to match the display to the independent

instrument.

5. Press ENTER to store the calibration.

6. Press the MODE key to return to RUN mode.

2-4 Direct Heat CO2 Incubator Thermo Scientific

 Section 2
Calibration

Relative Humidity If a reliable RH measuring device is not available, the display can be
(continued) calibrated to a typical level;

1. Follow the RH stabilization periods outlined above.

2. With a full humidity pan and stable temperature, the relative humidity
in the chamber will be 95%.

3. Using Step 3-5 of the relative humidity sensor adjustment, adjust the
display to 95%.

This calibration method should be accurate to within 5%.

Thermo Scientific Direct Heat CO2 Incubator 2-5

Section 2
Calibration

2-6 Direct Heat CO2 Incubator Thermo Scientific

 Section 3 Configuration
Several features available in Configuration mode allow custom setup of the
incubator. These features are listed with descriptions below. All features
may not be necessary in all applications, but are available if needed. To
enter Configuration mode, press the MODE key until the CON indicator
lights. Press the right and/or left arrow until the appropriate parameter
appears in the message display. See Chart 3-1 for more detail.

Turn Audible Alarm The audible alarm can be turned on or off. The factory setting is ON.
ON/OFF 1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until AUDIBLE XXX shows in the message
display.

3. Press the up/down arrow to toggle AUDIBLE ON/OFF.

4. Press ENTER to save the setting.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

New HEPA Filter When the REPLACE HEPA reminder displays and the visual alarm
flashes, the specified time has elapsed and the HEPA filter should be
replaced. To clear the display and reset the timer after replacing the HEPA
filter with a new one, follow the steps below.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until NEW HEPA shows in the message display.

3. Press ENTER to restart the timer and clear the REPLACE HEPA
alarm.

4. Press the MODE key to return to RUN mode.

Thermo Scientific Direct Heat CO2 Incubator 3-1

Section 3
Configuration

Set REPLACE HEPA A HEPA filter replacement timer can be set for a specific amount of time,
from 1 to 12 months of actual unit running time. Time will not accrue
filter reminder when the unit is turned off. The default time is 6 months. When the
allotted time runs out, REPLACE HEPA appears in the display and the
visual alarm flashes. To set the reminder, use the following procedure.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until REPL HEPA XX is displayed.

3. Press the up/down arrow to choose the number of months desired.

4. Press ENTER to save the number.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Note After the reminder has been set, check the allotted time remaining by
going to Configuration mode, then pressing the right arrow until NEW
HEPA XXX displays. This number is the remaining days before the filter
replacement time specified runs out. For example, if 12 months was chosen
in the REPL HEPA XX message display, the NEW HEPA number would
be 365 days. s

Set Access Code A 3-digit Access Code can be entered to avoid unauthorized personnel
from changing the setpoints, calibration, or configuration. A setting of
000 bypasses the access code. The factory setting is 000.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until ACC CODE XXX shows in the message
display.

3. Press up/down arrow to change the access code.

4. Press ENTER to save the access code.

5. Press the MODE key to return to the RUN mode or right/left arrow
to go to next/previous parameter.

3-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 3
Configuration

Set Low Temp Alarm The low temp alarm limit is the deviation from the temperature setpoint
which causes a low temp alarm. The low temp alarm is variable from 0.5°
Limit (tracking alarm) below setpoint to 5° below setpoint. The factory setting is 1° below
setpoint. A minus sign (-) in the display indicates that the alarm setting is
below the setpoint.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until TEMP LO -X.X shows in the message
display.

3. Press up/down arrow to change the low temp alarm limit.

4. Press ENTER to save the low temp alarm limit.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Enable Low Temp The low temperature alarm can be programmed to trip the remote alarm
contacts. A setting of ON allows this, an OFF setting blocks the low temp
Alarm to Trip Contacts alarm from tripping the contacts. The factory setting is ON.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until TMP RLY XXX displays.

3. Press the up/down key to toggle the setting ON/OFF.

4. Press ENTER to save the setting

5. Press the MODE key to return to RUN mode, or the right/left arrow
to go to next/previous parameter.

Thermo Scientific Direct Heat CO2 Incubator 3-3

Section 3
Configuration

Set Low CO2 Alarm The low CO2 alarm limit is the deviation from the CO2 setpoint which
will cause a low CO2 alarm. The setpoint is variable from 0.5% CO2
Limit (tracking alarm) below setpoint to 5.0% CO2 below setpoint. The factory setting is 1.0%
CO2 below setpoint. A minus sign (-) in the display indicates that the
alarm setting is below the setpoint.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until CO2 LO -X.X shows in the message
display.

3. Press up/down arrow to change the low CO2 alarm limit.

4. Press ENTER to save the low CO2 alarm limit.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Set High CO2 Alarm The high CO2 alarm limit is the deviation from the CO2 setpoint which
Limit (tracking alarm) will cause a high CO2 alarm. The setpoint is variable from 0.5% CO2
above setpoint to 5.0% CO2 above setpoint. The factory setting is 1.0%
CO2 above setpoint.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until CO2 HI X.X shows in the message display.

3. Press up/down arrow to change the high CO2 alarm limit.

4. Press ENTER to save the high CO2 alarm limit.

5. Press the MODE key to return to RUN mode, or right/left arrow to

go to next/previous parameter.

3-4 Direct Heat CO2 Incubator Thermo Scientific

 Section 3
Configuration

Enable CO2 Alarms to High and Low CO2 alarms can be programmed to trip the remote alarm
Trip Contacts contacts. A setting of ON allows this, a setting of OFF blocks CO2 alarms
from tripping the contacts. The factory setting is ON.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until CO2 RLY XXX shows in the message
display.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press ENTER to save the setting.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Set New Zero Number If a new T/C CO2 sensor is being installed, the two numbers on the
factory installed sticker on the T/C cell must be entered to calibrate CO2
for T/C CO2 Sensors in the unit.

Note For the technician’s convenience, a label containing the two

numbers on the T/C cell is affixed inside the electronics drawer. s

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until T/CZ# XXXX shows in the message
display.

3. Press up/down arrow to change the zero number to match the sticker.

4. Press ENTER to save the setting.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Thermo Scientific Direct Heat CO2 Incubator 3-5

Section 3
Configuration

Set New Span Number If a new T/C CO2 sensor is being installed, the two numbers on the
for T/C CO2 Sensors factory installed sticker on the T/C cell must be entered to calibrate the
CO2 in the unit.

Note For the technician’s convenience, a label containing the two numbers
on the T/C cell is affixed inside the electronics drawer. s

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until T/CS# XXXX shows in the message display.

3. Press up/down arrow to change the span number to match the sticker.

4. Press ENTER to save the setting.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Setting a Low RH On units with the RH option installed, a low RH alarm limit may be
Alarm Limit entered. The low RH alarm limit is the %RH in the cabinet that causes a
low RH alarm. The setpoint is variable from setpoint 0 to 90% RH. The
factory setting is 0% RH, which disables the alarm.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until RH LO XX shows in the message display.

3. Press up/down arrow to change the RH low alarm limit.

4. Press ENTER to save the RH low alarm limit.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

3-6 Direct Heat CO2 Incubator Thermo Scientific

 Section 3
Configuration

Enable RH Alarms to The low RH alarm can be programmed to trip the remote alarm contacts.
Trip Contacts A setting of ON allows this, a setting of OFF blocks the RH alarm from
tripping the contacts. The factory setting is ON.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until RH RLY XXX shows in the message
display.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press ENTER to save the setting.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Enabling Temp/RH to On units equipped with the RH option, the upper seven segment display
be Displayed on the control panel can be configured to display Temp continuously, RH
continuously, or toggle between Temp and RH. If the units does not have
RH, the upper display always shows temperature. If temperature is set to
ON, and the RH is set OFF, temperature shows continuously. If
temperature is set to OFF and RH is set to ON, RH shows continuously.
If both are turned ON, the display toggles between the two. The factory
setting defaults to toggle mode if the RH option is present.

1. Press the MODE key until the CON indicator lights.

2. Press the right arrow until DISP TMP XXX or DISP RH XXX shows
in the message display.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press ENTER to save the setting.

5. Press the MODE key to return to RUN mode or right/left arrow to go

to next/previous parameter.

Thermo Scientific Direct Heat CO2 Incubator 3-7

Section 3
Configuration

3-8 Direct Heat CO2 Incubator Thermo Scientific

 Section 3
Configuration

Thermo Scientific Direct Heat CO2 Incubator 3-9

Section 3
Configuration

3-10 Direct Heat CO2 Incubator Thermo Scientific

 Section 4 Alarms
The Series 8000DH incubator alarm system is shown in the table below.
When an alarm is active, the message appears in the LED message display.
Pressing disables the audible alarm for the ringback period. However,
the visual alarm continues until the incubator returns to a normal
condition. The alarms are momentary alarms only. When an alarm
condition occurs and then returns to normal, the incubator automatically
clears the alarm condition and the message display.
Table 4-1. Alarm and Description
Description Message Delay Ringback Relay
No alarm condition exists SYSTEM OK or CLASS 100 ----- ----- -----
Temp > Otemp Set point SYS IN OTEMP 0 min. 15 min. Yes
Air Temp Sensor Fault (See Section 4) AIR SNSR ERR 0 min. 15 min. No
Temperature Controller Failure (See Sect. 4) TMP CTRL ERR 0 min. 15 min. YES
CO2 Sensor Fault (See Section 4) CO2 SNSR ERR 0 min. 15 min. No
Replace HEPA filter reminder-set time expired
REPLACE HEPA 0 min. ----- No
(See Section 3)
Inner Door is Open DOOR OPEN 15 min. 15 min. No
CO2 is higher than CO2 High Tracking Alarm CO2 IS HIGH 15 min. 15 min. Programmable
CO2 is lower than CO2 Low Tracking Alarm CO2 IS LOW 15 min. 15 min. Programmable
TEMP is lower TEMP Low Tracking Alarm TEMP IS LOW 15 min. 15 min. Programmable
RH is lower than RH Low Limit Alarm (RH option) RH IS LOW 30 min. 15 min. Programmable

- All alarm delays and ringback times are ±30 seconds -

When multiple alarm conditions occur, active messages are shown in the
message display one at a time, updating at 5 second intervals. Pressing
during multiple alarms causes all active alarms to be silenced and to ring
back in 15 minutes.

The TEMP IS LOW alarm is disabled when the Temp set point is 10°C.
The CO2 alarms are disabled when the CO2 set point is 0.0%.

Thermo Scientific Direct Heat CO2 Incubator 4-1

Section 4
Alarms

Temp Controller Failure In addition to other safety features designed into Series 8000DH
incubators, a thermostat is provided to monitor the cabinet’s temperature.
TMP CNTR ERR In the unlikely event of a temperature control failure, the thermostat will
turn off all heaters at a cabinet temperature of 160°C, ±5%. This is
intended to be a safety feature to protect the incubator, and is not
intended to protect the cell cultures or the equipment inside the chamber
should a temperature control failure occur. Should such a failure occur,
contact the Technical Services Department or your local distributor.

Sensor Fault Alarms The microprocessor in Series 8000DH incubators continually scans all
available sensors to ensure that they are operating properly. Should an error
be detected, the incubator sounds an alarm and shows the appropriate
message. Contact your local distributor.

4-2 Direct Heat CO2 Incubator Thermo Scientific

 PREVENTIVE MAINTENANCE
Incubators
Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to keep your
unit functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For maximum performance
and efficiency, it is recommended the unit be checked and calibrated periodically by a qualified service technician.

The following is a condensed list of preventive maintenance requirements. See the specified section of the operating manual for further
details.

We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on Preventive
Maintenance or Extended Warranties, please contact Technical Services.

Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required.

Tips for all incubators:

Do NOT use bleach or any disinfectant that has high
Do not use powdered gloves for tissue cultures.
chloros

Use sterile, distilled or demineralized water.

Avoid spraying cleaner on the CO2 sensor.
 Preventive Maintenance for Series 8000DH Incubators

Refer to Manual Action Daily Weekly Monthly 3 to 6 Yearly

Section Months

-- Check CO2 tank levels.

-- Inspect door latch, hinges and door gasket seal.

1 Check water level in the humidity pan, ½” from top.

2 Verify and document CO2, humidity and temperature

calibration, as applicable (See Calibration).

Disinfect the interior of the incubator (See Routine

Maintenance).

1 Replace HEPA, access port filter, air sample filter, and

CO2 filters*, if applicable (or as needed).

5 Perform sterilization cycle as needed.

 Section 5 Routine Maintenance

Warning If the unit has been in service, turn it off and disconnect the
power cord connector before proceeding with any maintenance. s

Caution Before using any cleaning method except those recommended by

the manufacturer, users must check with the manufacturer that the
proposed method will not damage the equipment. s

Use an appropriate disinfectant. All articles and surfaces must be

thoroughly cleaned, rinsed with sterile water, and rough-dried.

Warning Alcohol, even a 70% solution, is volatile and flammable. Use it

only in a well-ventilated area that is free from open flame. If any
component is cleaned with alcohol, do not expose the component to open
flame or other possible hazard. Do not spray the T/C sensor with
flammable solutions. The internal temperature of the CO2 sensor is
approximately 150°C when the unit is in operation. Allow sufficient time
for the sensor to cool before cleaning.
Do not use strong alkaline or caustic agents. Stainless steel is corrosion
resistant, not corrosion-proof. Do not use solutions of sodium
hypochlorite (bleach) as they may also cause pitting and rusting. s

A HEPA filter replacement kit (see Parts List) should be on-hand prior to
cleaning the incubator interior.

Thermo Scientific Direct Heat CO2 Incubator 5-1

Section 5
Routine Maintenance

Clean Chamber 1. Remove the shelves, access port filter assembly, HEPA filter, air sample
Interior filter and tubing, and the left and right duct sheets. Discard the HEPA
filters and the access port filter assembly. See Figure 5-1.

Figure 5-1. Component Locations

2. Wash the air sample filter tubing, shelves, and ducts with disinfectant,
then rinse with sterile water. Optionally, the shelves and ducts may be
autoclaved.

3. Wash the inner door gasket with disinfectant. This gasket may be
removed to be cleaned, or replaced.

4. Wash the cabinet interior with disinfectant, starting at the top and
working down. Refer to the disinfectant directions for length of time
needed before rinsing. Wash the inner door both inside and out. The
cabinet and door must be rinsed with sterile water until the
disinfectant has been removed. After the cabinet has been rinsed, spray
with 70% alcohol.

5. Install the left and right ducts, inner door gasket, access port filter
assembly, and air sample filter and tubing, spraying each with 70%
alcohol.

6. Install a new HEPA filter.

7. Install the shelves and spray with 70% alcohol.

Clean Cabinet Clean the incubator exterior with a damp sponge or soft, well-wrung cloth
and mild detergent dissolved in water. Dry with a soft cloth.
Exterior

5-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 5
Routine Maintenance

Clean Glass Doors The chamber glass door and the optional independent inner glass doors
may be cleaned using the same disinfectant as used on the incubator
interior. It is imperative that they be rinsed with sterile distilled water to
remove the disinfectant residue. The doors should then be dried with a
soft cloth.

Some precautions in the cleaning and care of the incubator glass doors:
Moisture leaches alkaline materials (sodium, Na) from the surface of the
glass. Evaporation of the moisture concentrates the alkaline and may
produce a white staining or clouding of the glass surface. Cleaning
chemicals with a PH above 9 and heat (autoclaving) accelerate the
corrosion process. Therefore, it is very important to rinse and dry the glass
doors after cleaning. Autoclaving the glass doors should be avoided.

There is no simple method for repairing corroded glass. In most cases, the
glass must be replaced.

Clean Humidity Pan Clean the humidity pan with soap and water and a general-use laboratory
disinfectant. Rinse with sterile water and spray with 70% alcohol. The pan
may be autoclaved.

HEPA Filter Replace the HEPA filter when the REPLACE HEPA reminder is
displayed. The REPLACE HEPA reminder can be set to alarm after a
Maintenance specified time from 1 to 12 months. The reminder default is the factory
recommended setting of 6 months. For details, see Section 3.

Replace Power Fuses To access the only replaceable fuse in the incubator:

1. Turn off the incubator’s power switch and unplug the power cord.

2. Remove the two screws from the top of the control panel. Grasp the
control panel on each side and pull straight outward.

4. Figure 5-3 shows the location of the fuse. See Table 5-1 following for
replacement fuse specifications.

5. Secure the control panel and return the unit to service. If the fuse
blows after restoring power to the incubator, contact your local
distributor.
Table 5-1. Fuse Replacement Chart
Fuse Voltage Manufacturer’s P/N Amperage Rating Rupture Speed IEC Letter Code
230VAC BUSS GMC-0.5A 500mA Time-Lag T
115VAC BUSS GMC-1.0A 1.0 Amp Time-Lag T

Thermo Scientific Direct Heat CO2 Incubator 5-3

Section 5
Routine Maintenance

Replace Air Sample 1. Connect one end of the air sample filter to the hose barb on the
chamber ceiling.
Filter
2. Connect the other end of the filter to the metal tubing on the HEPA
filter adapter (Figure 5-2).

Figure 5-2. Connect to Metal Tubing

Electronics Section 1. Turn off the incubator’s power switch and unplug the power cord.

2. Remove the three screws from the back of the cabinet top.

3. Slide the top backward about an inch and lift it off.

5-4 Direct Heat CO2 Incubator Thermo Scientific

 Section 5
Routine Maintenance

Major Components Refer to Figure 5-3. Some components shown are factory installed options.

Figure 5-3. Electronics Drawer

Thermo Scientific Direct Heat CO2 Incubator 5-5

Section 5
Routine Maintenance

Sterilization Cycle

Information You Need to Know Before Starting a Cycle

• The Sterilization Cycle requires approximately 12 hours -

heat-up (2-4 hours), sterilize (2 hours), and cool down (6-8 hours).
Additional time is needed to verify the calibration of temperature and CO2 after the cycle is
complete.

• During the Sterilization Cycle, the incubator updates the temperature to the analog output
board and the 1535, however CO2 will be fixed at setpoint and RH will be fixed at RH
Low Limit plus 1%.

• A HEPA filter replacement kit (see the spare parts list) should be on hand prior to
initiating the Sterilization Cycle.

Information About the Cycle

• Pre-cleaning may be required. In order to avoid odors, stains on the interior, baked-on
material, etc., wipe off all visible signs of spills.

• Odor may occur during the Sterilization Cycle and is considered normal.

• The Steri Cycle is not intended to sterilize other items; instruments, etc., from the lab.

• During the cycle, the unit chamber becomes hot enough to melt samples, instruments,
dishes, etc., left inside the unit. The items listed below also need to be removed.

1) HEPA filter
2) Air sample filter
3) Access port filter assembly
4) Water in the humidity pan
5) Temp/RH recorder probe, if applicable
6) IR sensor, if applicable

• During the Sterilization Cycle, discoloration

of some materials may occur. For example,
stainless steel turns a straw color after a period
of exposure to high temperatures. This is
normal.

5-6 Direct Heat CO2 Incubator Thermo Scientific

 Section 5
Routine Maintenance

Checkpoints

What if? Then

Cycle does not initiate or terminates in mid-cycle

Check for alarms: SYS IN OTEMP, AIR SNSR ERR, TMP CTRL ERR

Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit.. Do
not use second unit during Sterilization Cycle. The Sterilization Cycle may be performed
on both units simultaneously, with proper electrical connection.

No action is taken within 1 minute, following display prompt

Unit returns to normal operation, SYSTEM OK

Need to cancel cycle in progress

Hold down green cycle initiation button for 3 seconds

Water is not removed from humidity pan during cycle

Steam is produced and may cause burns

Listed components are not removed before initiating cycle

Components cannot withstand the sterilization cycle temperatures and are destroyed

Unit is not powered off before removing the I/R sensor, if applicable
Damage to the sensor may result

Dummy I/R sensor is not installed

The sensor cable cannot withstand sterilization cycle temperatures and is destroyed
properly

The outer door is opened during the heat or sterilization phases

An outer door alarm occurs: CLOSE DOOR in the display, plus an audible (cannot be
silenced) and visual alarm.

The outer door is open longer than 20 seconds during phases listed above
Cycle is canceled, unit goes to CANCELED COOL PHASE*

The outer door is open during the cool down phase when the temp is 60°C or greater
An outer door alarm occurs

Power interruption during the HEAT PHASE

HEAT PHASE resumes if the chamber temperature was less than 90°C when power was
interrupted
HEAT PHASE resumes if the chamber temperature was greater than 90°C when power
was interrupted, and the temperature dropped less than 1°C
CANCELED COOL PHASE* starts if the chamber temp drop is more than 1°C

Power interruption during the STERILIZATION PHASE

HEAT PHASE begins if the chamber temperature has not dropped below 139°C
STERILIZATION PHASE starts again when the chamber temperature reaches 140°C
CANCELED COOL PHASE* starts if the chamber temp dropped below 139°C

* For further information on CANCELED COOL PHASE, see page 5-12.

Thermo Scientific Direct Heat CO2 Incubator 5-7

Section 5
Routine Maintenance

Sterilization Cycle Warning The Sterilization Cycle heats the incubator interior surfaces to
140°C. Contact with any surface inside the outer door during this cycle
may result in burns. s

1. Empty the water from the humidity pan and place the pan back into
the incubator. Remove any samples, instruments, dishes, etc. from the
chamber.

2. Press and hold the large white sterilization cycle button on the right
side of the unit (Figure 5-4) for approximately 3 seconds until the
LED lights.

Run Set Cal Config Heat Temp

Sterilization

~
~
~
Silence Mode Enter
Inject % CO 2
Scroll for Parameters

Cycle Button

Figure 5-4. Sterilization Button Location

3. ENTER the access code, if applicable. An access code is recommended

to prevent accidental cycle initiation.

4. Pre Sterilization with T/C CO2 sensor - The display toggles between
“REMOVE HEPAs”, “REMOVE WATER” and “PRESS ENTER”. If
ENTER is not pressed within 1 minute, the display returns to
“SYSTEM OK”.

5. Remove the HEPA filter, air sample filter and access port filter
assembly (Figure 5-5). Tubing can remain in the unit during the
sterilization cycle. In addition, remove the temp/RH recorder probe
and IR sensor, if applicable. Discard the filters.

Figure 5-5. Filter Locations

Pre Sterilization with IR CO2 sensor - If an IR sensor is connected,
the display toggles between “POWER OFF” and “REMOVE IR”. If
power is not turned off within 1 minute, the display returns to
“SYSTEM OK”.

5-8 Direct Heat CO2 Incubator Thermo Scientific

 Section 5
Routine Maintenance

Sterilization Cycle Note To remove the IR sensor, if applicable, first power the unit off. Then
(continued) turn the two 1/4-turn fasteners on the top duct until the cover plate is
disengaged. Secured on the inside of the plate is a dummy sensor (Figure
5-6). s

Figure 5-6. Dummy Sensor

Figure 5-7. IR Sensor with Cable

Grip the IR sensor and carefully pull it downward. Unscrew the cable
from IR sensor (Figure 5-13). Set the sensor aside. See Step 9 for the IR
sensor disinfection procedure. Connect the dummy sensor to the cable. Fit
the installed dummy sensor up into the previous sensor location.

When the power is turned on with the IR sensor removed, the display
toggles between “REMOVE HEPAs”, “REMOVE WATER”, and “PRESS
ENTER”. If “ENTER” is not pressed within 1 minute, the display toggles
between “POWER OFF” and “REPLACE IR”. When the unit is powered
back on with the IR sensor reinstalled, the display returns to “SYSTEM
OK”.

Figure 5-14. Displays with IR Sensor Removed

Thermo Scientific Direct Heat CO2 Incubator 5-9

Section 5
Routine Maintenance

Sterilization Cycle 6. After ENTER is pressed, the Heat Phase initiates. The white LED
flashes and the display toggles between “STERILIZING” and “HEAT
(continued) PHASE”. During this period, the HEAT light will be on and the
cabinet heats to sterilization temperature.

Figure 5-15. Heat Phase

7. When the air temperature in the unit reaches 140.0°C, the Sterilization
Phase begins and the display changes to “STERILIZING”.

Figure 5-16. Sterilization Phase

8. After approximately 2 hours, a 5-second audible tone sounds, signaling

that sterilization is complete. The Cool Phase begins. The display
toggles between “STERILIZING” and “COOL PHASE”.

Figure 5-17. Cool Phase

5-10 Direct Heat CO2 Incubator Thermo Scientific

 Section 5
Routine Maintenance

Sterilization Cycle 9. Completed Sterilization Cycle with T/C CO2 sensor - When the
(continued) temperature cools down to the original set operating temperature or
30°C, whichever is higher, the display toggles between “CYC
COMPLETE”, “REPL HEPAs”, and “PRESS ENTER”. The white
LED is lighted but no longer flashes.

Completed Sterilization Cycle with IR CO2 sensor - When the

temperature cools down to the original set operating temperature or
30°C, whichever is higher, the display toggles between “CYC
COMPLETE”, “POWER OFF”, and “REPLACE IR”. The white
LED is lighted but no longer flashes.

Clean the IR sensor with either isopropanol or Lysol No-Rinse Sanitizer.

When using isopropanol, simply spray the sensor (do not saturate) and
allow to dry. With Lysol, spray the sensor (do not saturate) and allow to
sit for a couple of minutes. Wipe dry with a clean, soft cloth.

Do not saturate the sensor or immerse the sensor in a cleaner.

Power the unit off, open the chamber, and remove the IR plate. Pull down
on the dummy, unscrew the cable from the top of the dummy and press
the dummy into the clip on the plate. See Figure 5-12. Screw the cable
onto the top of the IR sensor. The cable is keyed and can only be inserted
one way. Reinstall the sensor by pushing it as far as it will go into the hole.

After the unit is powered back on with the IR sensor reinstalled, the
display toggles between “CYC COMPLETE”, “REPL HEPAs”, and
“PRESS ENTER”.

Figure 5-18. IR Sensor Reinstalled

Thermo Scientific Direct Heat CO2 Incubator 5-11

Section 5
Routine Maintenance

Sterilization Cycle 10. Open the chamber and install a new HEPA filter, air sample filter and
(continued) access port filter assembly. Press ENTER.

11. When ENTER is pressed, the LED goes out, the display returns to
“SYSTEM OK”, and the HEPA filter replacement timer is reset.

12. Fill the humidity pan with 3 liters of sterile distilled water with the
recommended purity range (See Section 1).

13. Allow to stabilize for at least 12 hours at the required temperature and
CO2 level. It is recommended that the temperature and CO2
calibrations be verified periodically during the first week of returning
the unit to service.

Caution The high temperature sterilization cycle may cause the T/C CO2
sensor output to change significantly. (This is normal and does not
indicate damage to the sensor.) Therefore it is essential that the CO2
calibration is verified before returning the unit to service. s

If an independent instrument is not available to verify calibration, the

following procedure may be performed. After the Sterilization Cycle and
before CO2 is allowed to enter the cabinet;

1) Fill and install the humidity pan.

2) Allow to stabilize at the desired operating temperature for at least 12

hours.

3) Calibrate the display to 0.0%.

Note If the Cool Phase is canceled, the display toggles between “CYC
CANCELED” and “COOL PHASE”.

• With T/C CO2 sensor - When the temperature cools down to the
original set operating temperature or 30°C, whichever is higher, the
display toggles between “CYC CANCELED”, “REPL HEPAs”, and
“PRESS ENTER”. The white LED is lighted but no longer flashes.

• With IR CO2 sensor - When the temperature cools down to the

original set operating temperature or 30°C, whichever is higher, the
display toggles between “CYC CANCELED”, “POWER OFF”, and
“REPLACE IR”. The white LED is lighted but no longer flashes.
After the unit is powered back on with the IR sensor reinstalled, the
display toggles between “CYC CANCELED”, “REPL HEPAs” and
“PRESS ENTER”.

5-12 Direct Heat CO2 Incubator Thermo Scientific

 Section 6 Factory Options
A description of the factory option connections to external equipment
follows.

Remote Alarms A set of relay contacts is provided to monitor alarms through an RJ11
telephone style connector on the back of the cabinet. Refer to Figure 6-5
for the location of the alarm connector. The 12-foot telephone cord (P/N
190388) and RJ11-to-screw terminal conversion box (P/N 190392) are
available through your local distributor.

The remote alarm provides a NO (normally open) output, an NC

(normally closed) output and COM (common). Refer to Figure 6-1.

The contacts will trip on a power outage or an overtemperature condition.

The contacts may also be programmed to trip or not trip on temperature
alarms, CO2 alarms, and RH alarms. See Section 3, Configuration mode.

Figure 6-1. Remote Alarm Contacts

CAUTION
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)

Thermo Scientific Direct Heat CO2 Incubator 6-1

Section 6
Factory Options

Humidity Readout Series 8000DH incubators are equipped with a humidity sensor to
monitor the relative humidity inside the chamber. The sensor is located on
the chamber ceiling and provides a signal that is displayed in 1%
increments on the control panel. The humidity readout can display
continuously, or toggle with the temperature readout. In addition, a low
alarm limit can be set on the humidity readout to detect when the
humidity pan runs dry. Refer to Section 3, Configuration.

The sensor is capable of measuring relative humidity from 10% to 100%

with an accuracy of ±5% above 90%. See Section 2, Calibration, for
details on calibrating the humidity readout.

Factors which may affect humidity levels in the chamber are: the water
level in the humidity pan, frequency of door openings, and the fact that
the humidity pan is located on the bottom of the chamber versus on a
shelf.

Caution Incubators equipped with a T/C (thermal conductivity) CO2

sensor rely on a constant level of relative humidity to accurately measure
and control the CO2 concentration in the incubator. s

6-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 7 Specifications
*Specifications are based on a nominal voltage of 115V or 230V in ambients of 22°C
to 25°C.

Temperature
Control . . . . .±0.1°C Microprocessor PID Control
Setpoint . . . . . . . . . . .Digital - Touch pad, 0.1°C
Range . . . . . . . . . . . .+5°C above ambient to 50°C
Uniformity . . . . . . . . . . . . . . . . . .±0.3°C @ +37°C
Tracking alarm..User programmable (low) indicator
Overtemp . . Tracking, user programmable, action, and indicator
Display . . . . . . . . .Digital, LED, 0.1°C increments

Temperature Safety
Type . . . Extreme temperature safety, action, and indicator
Sensor . . Thermostat, independent of temp control system
Indicator . Message center, audible and visual alarms

Relative Humidity
Control . . . . .Humidity pan - natural vaporization
Humidity w/ pan . . . . . . . . . . . . .95% RH at 37°C
Display . . . . . . . . . . . . .Optional in 1% increments
Alarm . . . . . . .Low RH with optional RH monitor

CO2
Control . . . . .±0.1% microprocessor PID control
Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . .T/C or IR
Readability . . . . . . . . . . . . . . . . . . . . . . . . . . .0.1%
Range . . . . . . . . . . . . . . . . . . . . . . . . . . . .0 to 20%
Inlet pressure . . . . . . . . . . . . . . . . . .15 psig (1 bar)
Display . . . . . . . . . .Digital LED, 0.1% increments

Thermo Scientific Direct Heat CO2 Incubator 7-1

Section 7
Specifications

Shelves
Dimensions . . . . . . .18.5” x 18.5” (47cm x 47cm)
Construction . . Stainless steel (belt sanded, both sides)
Surface area . . . . .2.4 sq. ft. (0.22 sq. m.) per shelf
Max. per chamber . . . . . . . .38.4 sq. ft (3.6 sq. m.)
Loading . . . 35 lbs (16kg) slide in and out, 50 lbs (23kg) stationary
Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
Maximum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

Construction
Interior volume . . . . . . . . . . . .6.5 cu. ft. (184 liter)
Interior . . . . . .Type 304 stainless steel shiny finish
Exterior . . . . . . . . . . . . . . . .18 gauge cold roll steel
Outer door gasket . .Four-sided molded, magnetic Santoprene
Inner door gasket . . . . . . . . . . . . . . . .Bulb, silicone
Insulation . . . . . . . . . . . . . . . . . . . . . .Mineral wool

Fittings
Access port . .1-1/4” (32mm) removable silicone plug
CO2 inlet . . . . . . . . . . . . . . . .1/4” (6.4mm) barbed
Sample port . . . . . . . . . . . . .Front mounted barbed

Electrical
115 Volt models
. . . . . . . . .115VAC, 50/60 Hz, 1PH, 9.6 FLA
(Operating range 90-125VAC)
230 Volt models
. . . . . . . . . .230VAC, 50/60 Hz, 1 PH, 4.4 FLA
(Operating Range 180-250VAC)
Power switch/circuit breaker . . . 2 Pole, 12.0 Amp
Accessory outlet . . .Voltage equal to the cabinet input.
75 Watts maximum, 0.5ma leakage current
Alarm contacts . . Deviation of temperature, CO2, power, NO and NC

7-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 7
Specifications

Dimensions
Exterior . . . . . . . . .26.1” W x 39.5” H x 25.0” F-B
. . . . . . . . . . . . . . .(66.3cm x 100.3cm x 63.5cm)
Interior . . . . . . . . .21.3” W x 26.8” H x 20.0” F-B
. . . . . . . . . . . . . . . .(54.0cm x 68.1cm x 50.8cm)

Weight
Net . . . . . . . . . . . . . . . . . . . . . . . . .260 lbs (118kg)
Net shipping . . . . . . . . . . . . . . . . .315 lbs (143kg)

Certifications
Refer to the Declaration of Conformity at the end of this manual

Safety Specifications
Indoor Use Only
Altitude . . . . . . . . . . . . . . . . . . . . . . .2,000 meters
Temperature . . . . . . . . . . . . . . . . . . . .5°C to 40°C
Humidity . . . . . . . . . . .80% RH at or below 31°C,
. . . . . . . .decreasing linearly to 50% RH at 40°C
Mains Supply Fluctuations
Mains supply voltage fluctuations not to exceed ±10%
of nominal voltage
Installation Category II1
Pollution Degree 22
Class of EquipmentI

__________________________________________________________
1 Installation category (overvoltage category) defines the level of transient overvoltage
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its overvoltage protection means. For example, in CAT II which is
the category used for instruments in installations supplied from a supply comparable to
public mains such as hospital and research laboratories and most industrial laboratories,
the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V
supply.

2 Pollution Degree describes the amount of conductive pollution present in the

operating environment. Pollution degree 2 assumes that normally only non-conductive
pollution such as dust occurs with the exception of occasional conductivity caused by
condensation.

Thermo Scientific Direct Heat CO2 Incubator 7-3

 Section 8 Spare Parts
Part Number . . . . . . . . . . . . . . . . . . . . . . . . .Description
190699 . . . . . . . . . . . . . . . . . . .Removable feather gasket
190670 . . . . . . . . . . . . .Duct sheet and shelf channel kit
224175 . . . . . . . . . . . . . . . . . . . . . . . .Stainless steel shelf
190884 . . . . . . . . . . . . .Stainless steel shelf and channels
191650 . . . . . . . . . . . . . . . .Micro board, (main control)
230135 . . Fuse, 1A, TD, 5 x 20mm (accessory outlet, 115V)
230120 . . .Fuse, 0.5A TD, 5 x 20mm (accessory outlet 230V)
430109 . . . . . . . . . . . . . . . . . . . . . . .Cordset, 10A, 250V
156117 . . . . . . . . . . .Motor, 2-pole, 115VAC, 50/60 Hz
190793 . . . . . . . . . . . . . . . . . . . . . . . . . . . .Display board
290184 . . . . . . . . . . . . . .Probe, 2252 Ohm/25°C, 1/8 x2
130077 . . . . . . . . . . . . . . . . . . . . .Stopper with 3/8” hole
290168 . . Thermal conductivity (T/C) type CO2 sensor
231204 . . . . . . . . . . . . . . . . . . .Infrared type CO2 sensor
2270112 . . . . . . . . . .CO2 valve kit with assorted fittings
965010 . . . . . . . . . . . . . . . . . . . . . . . . .CO2 gas regulator
3166242 . . . . . . . . . . . . . . . . . . . . . . . .Gas shuttle switch
3050 . . . . . . . . . . . . . . . . . . . . . . . . . . .External gas guard
950316 . . . . . . . . . . . . . . . . . .Wall clamp for CO2 bottle
231203 . . RH sensor assembly (P/N 1900091 RH display option)
230180 . . . . .12A, DPDT circuit breaker switch (power)
400119 . . . . . . . . . . . . . . . . .Switcher, 40W, 12, 5, -12V
196018 . . . . .34 position control to display ribbon cable
2270111 . . . . . . . . . . . . . . . . . . . . . . . .Inner door switch
360283 . . . . . . . . . . . . . . . . . . . . . . . .Outer door switch
360236 . . . . . . . . . . . . . . . . .Sterilization initiation switch
100115 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Blower wheel
190985 . . . . . . . . . . . . . . . . . .Access port filter assembly
760210 . . . . . . . . . . . .Disposable in-line filters (10 filters)
770001 . . Disposable filter, 99.97% (CO2 line, air sample)
760175 . . . . . . . . . . . . . . . . . .HEPA filter, 5.5” diameter
760209 . . . . . . . . . . . . . . . . . .HEPA value pack (4 filters)
1900067 . . . . . . . . . . . . . . . .HEPA filter replacement kit
190650 . . . . . . . . . . . . . . . . . .Independent inner door kit
190666 . . . . . . . . . . . . . . . . . . . . .Right hand door swing
1900063 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Roller base
140387 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Overlay
50109579 . AquaTec water cleaning cell 4-pack for water reservoirs, pans, bottles)
1900346 . . . . . . . . . . . . . . . . . . . . .Display control board
129060 . . . . . . . . . . . . .Spring (1) for updated duct sheets
114021 . . . . . . . . . .Grommet (1) for updated duct sheets
1900567 . . . . . . . . . . . . . . . .Exterior door assembly SRO

Thermo Scientific Direct Heat CO2 Incubator 8-1

Section 8
Spare Parts

8-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 8
Spare Parts

Thermo Scientific Direct Heat CO2 Incubator 8-3

Section 8
Spare Parts

8-4 Direct Heat CO2 Incubator Thermo Scientific

 Section 8
Spare Parts

370-200-5 Rev 8
370 BACK PANEL ASSEMBLY Page 1 of 2

Thermo Scientific Direct Heat CO2 Incubator 8-5

Section 8
Section title

370-200-5 Rev 8
370 BACK PANEL ASSEMBLY Page 2 of 2

8-6 Direct Heat CO2 Incubator Thermo Scientific

 Section 8
Section title

Thermo Scientific Direct Heat CO2 Incubator 8-7

Section 8
Spare Parts

8-8 Direct Heat CO2 Incubator Thermo Scientific

 Section 8
Spare Parts

Thermo Scientific Direct Heat CO2 Incubator 8-9

Section 8
Spare Parts

8-10 Direct Heat CO2 Incubator Thermo Scientific

 Section 8
Spare Parts

Thermo Scientific Direct Heat CO2 Incubator 8-11

 Section 9
Electrical Schematics

Thermo Scientific Direct Heat CO2 Incubator 9-1

Section 9
Electrical Schematics

9-2 Direct Heat CO2 Incubator Thermo Scientific

 Section 9
Electrical Schematics

Thermo Scientific Direct Heat CO2 Incubator 9-3

Section 9
Electrical Schematics

9-4 Direct Heat CO2 Incubator Thermo Scientific

 Section 9
Electrical Schematics

Thermo Scientific Direct Heat CO2 Incubator 9-5

Section 9
Electrical Schematics

9-6 Direct Heat CO2 Incubator Thermo Scientific

 THERMO FISHER SCIENTIFIC STANDARD PRODUCT WARRANTY
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go
into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first year
warranty period.

During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense, labor
included. Installation and calibration are not covered by this warranty agreement. The Technical Services Department must be contacted for warranty
determination and direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.

Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the compo-
nent part beyond the original warranty period. The Technical Services Department must give prior approval for return of any components or equipment.
At Thermo's option, all non-conforming parts must be returned to Thermo Fisher Scientific postage paid and replacement parts are shipped FOB desti-
nation.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO WARRANTIES
OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential
damages including, without limitation, damages relating to lost profits or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction man-
uals carefully detail equipment installation, operation and preventive maintenance.

If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or 1-740-373-4763. We're ready
to answer your questions on equipment warranty, operation, maintenance, service and special application. Outside the USA, contact your local dis-
tributor for warranty information.

ISO
9001
Rev. 4 4/09 REGISTERED
 THERMO FISHER SCIENTIFIC INTERNATIONAL DEALER WARRANTY
The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows for shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protec-
tion extends to any subsequent owner during the first year warranty period. Dealers who stock our equipment are allowed
an additional six months for delivery and installation, provided the warranty card is completed and returned to the Technical
Services Department.

During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced
at Thermo's expense, labor excluded. Installation and calibration are not covered by this warranty agreement. The Technical
Services Department must be contacted for warranty determination and direction prior to performance of any repairs.
Expendable items, glass, filters, reagents, tubing, and gaskets are excluded from this warranty.

Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give prior
approval for return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.

Contact your localdistributor for warranty information. We’re ready to answer your questions on equipment warranty, oper-
ation, maintenance, service and special application.
ISO
9001
Rev. 4 2/09 REGISTERED
Thermo Fisher Scientific
401 Millcreek Road
Marietta, Ohio 45750
United States

www.thermofisher.com