Onion Cheek Lab
Onion Cheek Lab
Objectives:
Students will discover that onions are made up of
cells. Students will observe onion cells under a
microscope. Students will discover that their skin is
made up of cells. Students will observe cheek cells
under a microscope.
Materials:
microscope
two glass slides
iodine stain
methylene blue stain
two cover slips
an onion
a toothpick/cotton bud
dropper
Procedures:
Onion cells
1. Peel a translucent piece of tissue from the onion. (The smaller the piece
the better.) Translucent means that you can see light through the specimen,
but it is not transparent.
2. Place the piece of onion on a glass slide and add a drop of the iodine
solution. Cover the slide with a cover slip using your best wet-mount making
techniques.
3. Observe the onion cell under both low and high power. Make a drawing of
one onion cell, labeling all of its parts as you observe them.
(At minimum, you should observe the nucleus, cell wall, and cytoplasm.)
Cheek cells
1. To view cheek cells, gently scrape the inside lining of your cheek with a
toothpick. DO NOT GOUGE THE INSIDE OF YOUR CHEEK! (We will
observe blood cells in a future lab!!)
2. Gently roll and rub the toothpick onto the top of a glass slide in an area
that will be visible through the microscope.
3. Add a drop of methylene blue (specific for animals) and cover with a cover
slip.
4. Observe the cheek cells under both low and high power of your
microscope. Draw a diagram of one cheek cell and label the parts.
(You should observe the cell membrane, nucleus, and cytoplasm.)
Name Date
Observation:
The following labeled drawings must be completed.
Drawings MUST be completed neatly using a pencil/colored pencil.
1. Onion Cell drawing (high power)
2. Cheek cell drawing (any power but preferably high)
Complete the following chart: The Cell organelle found in plant, animal,
or both. (See the diagrams) (Place an x in the appropriate box)
Organelle Plant Animal Function (look these up online)
Nucleus
Cell Wall
Chloroplast
Cytoplasm
Cell
Membrane
Name Date