Mariano L.

Sindac Integrated School

THE EFFECTIVENESS OF ALAGAW LEAVES (Premna odorata) AS ANTI

PARASITIC DOG SHAMPOO

Ronjake M. Magos, Princess Venice M. Pornias, Jamaeica A. Salinas, Greg John Paul T.
Suridor.1, Jenny C. Basada.2
1
Student Researcher, Junior High School Department, Mariano L. Sindac Integrated School,
Sitio Puntian, Brgy, Zabali Baler, Aurora
2
Junior High School Research Adviser, Junior High School Department, Mariano L. Sindac
Integrated School, Sitio Puntian, Brgy, Zabali Baler, Aurora

INTRODUCTION

Background of the Study

Several years dog has found that effect in the most problem, many owner of dog not
even take a solution of the dog skin because they did not know how to solve it in easy way, in
dog skin they can get a more infection if we did not care of it.We all know that dog, they can
play whenever in place, but in that way we don’t even know, that our dog they can get a more
parasites. For those people, who loves a dog to take care of them, it can buy a dog food to
prevent a serious illness, they can afford to buy a dog shampoo to prevent a parasites,
everyday they bath the dog or sometimes not allowed to leave the house, and sometimes our
pet dog sleep with us, play with us. But not at all people treated their dog like this. Because
other people who have a pet dog are sometimes just left to roam anywhere, the type that only
takes care of a dog, to have a watchman in their house. But in this happens, that you are
comfortable and able to take outside you dog, but you didn’t even know, they’re a more
parasites, then sometimes only bath the pet dog once a month, these dogs are easy to often
seen with fleas, and it’s very dangerous not only in a dog, but even in people. Because flea are
not just an animal living in dog skin, many effect that they can take over in our own dog.
Because of many dog are just neglected by their boss, and fleas arre also increasing, with the
over-increasing number of fleas on the dog. Many company that decides to create a solution
fight for fleas, but some other ways, they not overcome solution of these problem in dog skin.
That’s why we need to take a study about how to solve the flea in dog, to get a more studies
about flea in dog, and it’s only for dog but to decrease a flea, and to make a better life, and
enjoy with our own dog. This studies is not a acid to get a harmful effect in your dog, because
of this studies we used a more natural ingredients to get a more healthy dog skin in our
environment.

According to parasite and vectors the place are considered the most important
ectoparasites it concerns to a range of animal include dogs(Nguyen, Colella et. Al, 2020). Flea
are they find in species anima, and flea may play in different role as parasites, and it can be an
ectoparasitic nuisance in animals. Some of the studies flea develop from eggs, especially in
Females lay up into 25 eggs per day, and it may be developed in hundreds during their life.
According in data, that fleas are also found in dogs over the world (Table 1). It recognized in
published literature of the years 1980 to 2010 for occurance and frequency of fleas in the dog
population of the different countries (Blanvillain, Porch, Hoyt., 2011). Fleas are also very
closed in dogs and it may bridging vector of ectoparasites, which they might transmit while
blood – bleeding. We can say that flea are very harmful, when it comes in the dog skin, and we
can’t avoid as they we owned dog, especially if the dog is hairy, it always target in every fleas.
The importance knowing the distribution of flea species on dogs and the factors there
distribution.

Statement of the problem

This study aims to explore the efficacy of alagaw leaves (Premna odorata) as an anti-parasitic
agent in dog shampoo formulations. Given the growing concern about parasitic infestations in
dogs and the increasing interest in natural alternatives, this research endeavors to assess the
potential of Alagaw leaves in managing parasitic challenges in dog care. The significance of
this research lies in its potential utility if successful, it promises to provide an interesting and
valuable contribution to the field, prompting our decision to undertake this investigation.

Formulation of the Hypothesis

As a student, I assume that extract of Alagaw leaves is works as Anti-parasitic dog shampoo
which can kill more flea.

Yes, we are also talking about dog skin and I hope that using this, as this invented liquid dog
shampoo for dog skin will not get anymore flea and they cannot harm the dog skin.

Significance of the study

This study aims to explore the efficacy of Alagaw leaves (Premna odorata) as an anti-parasitic
agent in dog shampoo formulations. The result of this study will be useful to the following:

1. Pet care industry- Companies in the pet care industry may find value in developing
new dog shampoo formulations with alagaw leaves to meet the demand for natural and
efficient anti-parasitic products.

2. Pet owners in general- Individuals who own dogs can benefit from a potential
alternative to conventional anti-parasitic products, potentially reducing reliance on
chemical-based treatments.

3. Local Communities- Local communities where alagaw plants are abundant may
benefit economically if the cultivation and processing of alagaw leaves for anti-parasitic
purposes become a viable industry.

4. Consumers- Especially for the consumers who are looking for a different alternative
product that is safe and organic for personal or household use may benefit from the
availability of alagaw-based anti-parasitic products, contributing to consumer choices
aligned with sustainability and well-being.

Scope and Limitation of the Study

This study aims to investigate the anti-parasitic properties of Alagaw leaves (Premna odorata)
in dog shampoo, with a focus on common dog parasites, and to explore its potential as a
natural and eco-friendly alternative to commercial anti-parasitic shampoos. This research is
also to help our community or other communities and others or some individuals will also get
benefit from this product.

Definition of Terms
Alagaw (Premna odorata) extracted from the Premna odorata plant, act as a natural and herbal
substitute for creating an anti-parasitic dog shampoo.Recognized for their medicinal qualities,
these leaves are utilized for their ability to address parasitic infestations in dogs, presenting a
chemical-free option for pet hygiene. The anti-parasitic properties of alagaw leaves work to
repel and eradicate typical dog parasites, offering a holistic and eco-friendly approach to
caring for pets.
 Time and place of the study
The collection of plant samples was conducted within the Sabang Poblacion Baler, Aurora.
The laboratory procedures were conducted in the Mariano Lumasac Sindac Integrated School
at Barangay Zabali Baler, Aurora from April 2024 . The time frame of this study was from
school years 2023-2024 and. The testing of the Alagaw leaves extracts and Anti-parasitic dog
bar shampoo assays were done at Mariano Lumasac Sindac Integrated School.

METHODS
Collection of Plant Samples
Plant 1: Alagaw leaves ( Premna odorata )
The two kilograms of Alagaw leaves ( Premna odorata ) that was used were collected
in Purok 4 Barangay Pingit, Baler, Aurora on April 20, 2024, at around 12:50 am in the
morning. The leaves were placed plastic tray

Figure 1.1 Place of Collection Figure 1. Collected Plant

Sample

II. Preparation of Plant Sample

Plant 1: Alagaw leaves (Premna odorata)
The collected alagaw leaves were weighed and washed with tap water, followed with
distilled water to avoid possible contamination of the sample.
 A B

III. Extraction Proces

Plant 1: Alagaw Leaves (Premna odorata)
The leaves were then cut and placed in a blender with ½ cup of water. After the
process of pulverization, the pieces were squeezed with a cloth to remove more liquid. After
removing excess liquid, it was poured into a cube to get a bar soap.

A B C

CHAPTER II.

Related Literature and studies

Alagao (Premna odorata), is a Anti-microbial Plant Extracts. Alagaw leaves it may can be used
as medicinal. For this study we used Alagao leaves in Antih-parasitic for a dog flea. Alagao
Premna odorata, is a native tree of the Philippines and locally known as “Alagaw” . Many
properties of the leaves extract were prepared a positive and negative controls. It can be found
anywhere. Most people didn’t know about this plant because they thought it’s just a grass and
useless.
Chemical components
Twenty five compounds were representing 72.15% a total compounds mainly of fatty acids and
easter forms (57.18%).Linolenic acid identified compounds representing 18.58%.Alagaw
leaves, scientifically identified as Premna odorata, encompass a range of chemical constituents
such as alkaloids, flavonoids, tannins, and essential oils. These elements play a role in the
plant’s potential medicinal properties and antioxidant effects.

Related Studies Local

This study aimed to find out whether Alagaw leaves can potentially act as a reducing agent for
the biosynthesis of silver nanoparticles, and whether the concentration of the leaves extracts
can affect. In another way, Flea Allergic Dermatitis (FAD) common in dogs and cats has been
successfully treated and tapeworm, infestations prevented with a number of new products being
used to control fleas. This study may understudied. Some of the different countries, the
Philippines with cats more risk of infection than dogs. In other country like Philippines is take
as under the context of previous multicenter survey. Ticks and fleas (20%) were randomly
selected from each tick/flea species and from each infested animal in studied countries.

Related Studies Foreign

In other study from foreign. According too Malaysia they revealed a relatively high rate of flea
infestation on 306 cats (71.38%), and all of them are identified. There must a higher flea
prevalence was also observed in female cats (77.99%). The high infestation of fleas in this
study is indicative of cats as a fleas reservoir than dogs (Azrizal-Wahid, Sofian-Azirun 2019).
A review of flea from India. Currently, about 2,700 flea species were described in the world.
Say that found a common flea Xenopsylla cheopis throughout in India (Rajamannar, Kuma et
al., 2022).

CHAPTER III.

Methodology

The subject of the study

The subject of the study, is to made a dog shampoo for flea and we used Alagao leaves (Premna
odorata), to friendly environment health skin disease. This study will make a procedure to on
how we make a dog shampoo. This study can do better of our dog fight from flea and to
prevent it.
The procedure

Ingredients: 50 Cup Apple Cider

50 Cup Water
30 Dish Soap
Alagaw leaves extracts

Treatment 1

Step 1: Find Alagao leaves in Sto. Puntian Barangay Zabali Baler, Aurora

Step 2: Took Alagao leaves from tree

Step 3: Prepare all the ingredients for making dog shampoo

Step 4: Start making dog shampoo using the ingredients

Step 5: Then, make three treatments, the first treatment is 10 mL

Step 6: Apply the 10 mL dog shampoo and number of minutes

Treatment 2

Step 1: Find Alagao leaves in Sto. Puntian Barangay Zabali Baler, Aurora

Step 2: Took Alagao leaves from tree

Step 3: Prepare all the ingredients for making dog shampoo

Step 4: Start making dog shampoo using the ingredients

Step 5: Then, make three treatments, the first treatment is 20 mL

Step 6: Apply the 20 mL dog shampoo and number of minutes

Treatment 3

Step 1: Find Alagao leaves in Sto. Puntian Barangay Zabali Baler, Aurora

Step 2: Took Alagao leaves from tree

Step 3: Prepare all the ingredients for making dog shampoo

Step 4: Start making dog shampoo using the ingredients

Step 5: Then, make three treatments, the first treatment is 50 mL

Step 6: Apply the 50 mL dog shampoo and number of minutes

Research design

Statistical Treatment

Treatment 1
We are going to apply 3 tbsp of Alagaw leaves extracts, 3 tbsp of Dish Soap, 5 tbsp Water, 5
tbsp Apple Cider

Treatment 2
We are going to apply 6 tbsp of Alagaw leaves extracts, 6 tbsp of Dish Soap, 10 tbsp Water, 10
tbsp Apple cider

Treatment 3
We are going to apply 12 tbsp of Alagaw leaves extracts, 12 tbsp of Dish soap, 20 tbsp Water,
20 tbsp Apple cider