February 2020

QIAamp® Fast DNA

Stool Mini Handbook
For fast purification of genomic DNA from stool samples

Sample to Insight__
Contents
Kit Contents ............................................................................................................... 4

Shipping and storage .................................................................................................. 4

Intended Use .............................................................................................................. 5

Safety Information ....................................................................................................... 6

Quality Control........................................................................................................... 6

Introduction ................................................................................................................ 7

Principle and procedure .................................................................................... 7

Sample size ..................................................................................................... 8

Lysis and removal of impurities in InhibitEX Buffer ............................................... 10

Purification on QIAamp spin columns................................................................ 10

Automated purification of DNA on QIAcube Instruments ..................................... 11

Equipment and Reagents to Be Supplied by User .......................................................... 13

Important Notes ........................................................................................................ 14

Preparation of reagents ................................................................................... 14

Handling of QIAamp Mini spin columns ............................................................ 16

Centrifugation ................................................................................................ 17

Protocol: Isolation of DNA from Stool for Human DNA Analysis ..................................... 18

Protocol: Using Stool Tubes for Isolation of DNA from Stool for Human DNA Analysis ...... 21

Protocol: Isolation of DNA from Stool for Pathogen Detection ......................................... 24

Protocol: Using Stool Tubes for Isolation of DNA from Stool for Pathogen Detection .......... 27

Protocol: Isolation of DNA from Larger Volumes of Stool................................................ 30

2 QIAamp Fast DNA Stool Mini Handbook 02/2020

Troubleshooting Guide .............................................................................................. 32

Appendix: Determination of Concentration, Yield, Purity and Length of DNA ................... 37

Ordering Information ................................................................................................ 39

Document Revision History ......................................................................................... 41

QIAamp Fast DNA Stool Mini Handbook 02/2020 3

Kit Contents
QIAamp Fast DNA Stool Mini Kit (50)
Catalog no. 51604
Number of preps 50

QIAamp Mini spin columns 50

Collection Tubes (2 ml) 200

InhibitEX® Buffer 140 ml

Buffer AL* 33 ml

Buffer AW1* (concentrate) 19 ml

Buffer AW2† (concentrate) 13 ml

Buffer ATE 12 ml

Proteinase K 1.4 ml

Quick-Start Protocol 1

* Contains chaotropic salt. Take appropriate laboratory safety measures and wear gloves when handling. Not
compatible with disinfecting agents containing bleach. See page 6 for safety information.
†
Contains sodium azide as a preservative.

Shipping and storage

The QIAamp Fast DNA Stool Mini Kit is shipped at room temperature (15–25°C) and can be
stored dry at room temperature for up to 1 year without showing any reduction in performance,
if not otherwise stated on the label. The QIAamp Fast DNA Stool Mini Kit contains a ready-to-
use proteinase K solution, which is supplied in a specially formulated storage buffer. Proteinase
K is stable for at least 1 year after delivery when stored at room temperature, if not otherwise
stated on the label. For storage longer than 1 year or if ambient temperature often exceeds
25°C, we suggest storing proteinase K at 2–8°C.

4 QIAamp Fast DNA Stool Mini Handbook 02/2020

Intended Use
The QIAamp Fast DNA Stool Mini Kit is intended for molecular biology applications. This
product is not intended for the diagnosis, prevention or treatment of a disease.

QIAcube® Connect is designed to perform fully automated purification of nucleic acids and
proteins in molecular biology applications. The system is intended for use by professional users
trained in molecular biological techniques and the operation of QIAcube Connect.

All due care and attention should be exercised in the handling of the products. We recommend
all users of QIAGEN® products to adhere to the NIH guidelines that have been developed for
recombinant DNA experiments or to other applicable guidelines.

QIAamp Fast DNA Stool Mini Handbook 02/2020 5

Safety Information
When working with chemicals, always wear a suitable lab coat, disposable gloves and
protective goggles. For more information, please consult the appropriate safety data sheets
(SDSs). These are available online in convenient and compact PDF format at
www.qiagen.com/safety where you can find, view and print the SDS for each QIAGEN kit
and kit component.

CAUTION
DO NOT add bleach or acidic solutions directly to the sample-
preparation waste.

The sample-preparation waste contains guanidine hydrochloride from Buffers AL and AW1,
which can form highly reactive compounds when combined with bleach.

If liquid containing these buffers is spilt, clean with suitable laboratory detergent and water. If
the spilt liquid contains potentially infectious agents, clean the affected area first with
laboratory detergent and water, and then with 1% (v/v) sodium hypochlorite.

Quality Control
In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of QIAamp
Fast DNA Stool Mini Kit is tested against predetermined specifications to ensure consistent
product quality.

6 QIAamp Fast DNA Stool Mini Handbook 02/2020

Introduction
The QIAamp Fast DNA Stool Mini Kit provides fast and easy purification of total DNA from
fresh or frozen stool samples. The purified DNA is of high quality and well suited for use in
PCR and other downstream applications.

The simple QIAamp spin procedure yields pure DNA ready for direct use in as little as
25 minutes. The procedure can be automated on QIAcube Connect for increased
standardization and ease of use (see page 11). Purification requires no phenol–chloroform
extraction or alcohol precipitation and involves minimal handling. DNA is eluted in low-salt
buffer and is free of protein, nucleases and other impurities or inhibitors. The purified DNA is
ready for use in PCR and other enzymatic reactions, or can be stored at –30°C to –15°C for
later use.

Stool samples typically contain many compounds that can degrade DNA and inhibit
downstream enzymatic reactions. To ensure removal of these substances, the QIAamp Fast
DNA Stool Mini Kit contains InhibitEX Buffer, which is specially formulated to separate
inhibitory substances from DNA in stool samples.

Principle and procedure

The QIAamp Fast DNA Stool Mini Kit is designed for rapid purification of total DNA from up
to 220 mg stool and is suitable for both fresh and frozen samples. A special protocol is
provided for isolating DNA from larger amounts of stool. The fast and easy procedure
comprises the following steps:

 Lysis of and separation of impurities from stool samples in InhibitEX Buffer

 Purification of DNA on QIAamp Mini spin columns (see flowchart, page 9)

QIAamp Fast DNA Stool Mini Handbook 02/2020 7

Sample size

The QIAamp Fast DNA Stool Mini Kit is optimized for use with 180–220 mg fresh or frozen
stool, but can also be used with larger amounts of stool. Starting with larger amounts of stool
is recommended when the target DNA is not distributed homogeneously throughout the stool
and/or is at a low concentration. A larger amount of starting material will increase the
likelihood of purifying DNA from low-titer sources in stool samples. See “Protocol: Isolation of
DNA from Larger Volumes of Stool”, page 30.

The QIAamp Fast DNA Stool Mini protocols can also be used for samples of less than 180 mg
(e.g., forensic samples). In such cases, follow one of the standard protocols, without reducing
the amounts of buffers used.

For maximum flexibility, protocols are provided for use with samples collected in both 2 ml
microcentrifuge tubes and 15 ml stool tubes.

8 QIAamp Fast DNA Stool Mini Handbook 02/2020

QIAamp Fast DNA Stool Mini Handbook 02/2020 9
Lysis and removal of impurities in InhibitEX Buffer

In the first steps of the protocol, stool samples are lysed in InhibitEX Buffer. Human cells lyse
efficiently at room temperature (15–25°C). Bacterial cells and those of other pathogens in the
stool are effectively lysed by incubating the stool homogenate at 70°C (if necessary, this
temperature can be increased to 95°C). This is recommended for detection of cells that are
difficult to lyse (e.g., some bacteria and parasites).

During lysis, DNA-degrading substances and PCR inhibitors present in the stool sample are
separated from the DNA by the InhibitEX buffer. The sample matrix is pelleted by centrifugation
and the DNA in the supernatant is purified on QIAamp Mini spin columns.

Purification on QIAamp spin columns

The QIAamp DNA purification procedure involves digestion of proteins, binding DNA to the
QIAamp silica membrane, washing away impurities and eluting pure DNA from the spin
column.

Proteins are digested and degraded under denaturing conditions during a 70°C incubation
with proteinase K. Buffering conditions are then adjusted to allow optimal binding of DNA to
the QIAamp membrane, and the sample is loaded onto the QIAamp spin column. DNA is
adsorbed onto the QIAamp silica membrane during a brief centrifugation step. Optimized salt
concentrations and pH conditions in the lysate ensure that remains of digested proteins and
other impurities, which can inhibit PCR and other downstream enzymatic reactions, are not
retained on the QIAamp membrane.

DNA bound to the QIAamp membrane is washed in two centrifugation steps. Optimized wash
conditions using two wash buffers ensure complete removal of any residual impurities without
affecting DNA binding.

10 QIAamp Fast DNA Stool Mini Handbook 02/2020

Purified, concentrated DNA is eluted from the QIAamp Mini spin column in low-salt buffer
equilibrated to room temperature (15–25°C). DNA yield is typically 10–50 µg but, depending
on the individual stool sample and the way it was stored, may range from 5–100 µg. DNA
concentration is typically 50–250 ng/µl. The eluted DNA is up to 20 kb long and is suitable
for direct use in PCR and other enzymatic reactions. If the purified DNA is to be kept, storage
at –30°C to –15°C is recommended.

The convenient spin procedure is designed to ensure that no sample-to-sample cross-

contamination occurs and to allow safe handling of potentially infectious samples.

QIAamp spin columns fit into most standard microcentrifuge tubes. Eluted DNA is collected in
standard 1.5 ml microcentrifuge tubes (not provided).

Automated purification of DNA on QIAcube Instruments

Purification of DNA can be fully automated on QIAcube Connect or the classic QIAcube. The
innovative QIAcube instruments use advanced technology to process QIAGEN spin columns,
enabling seamless integration of automated, low-throughput sample prep into your laboratory
workflow. Sample preparation using QIAcube instruments follows the same steps as the
manual procedure (i.e., lyse, bind, wash and elute), enabling you to continue using the
QIAamp Fast DNA Stool Mini Kit for purification of high-quality DNA.

QIAcube instruments are preinstalled with protocols for purification of plasmid DNA, genomic
DNA, RNA, viral nucleic acids and proteins, plus DNA and RNA cleanup. The range of
protocols available is continually expanding, and additional QIAGEN protocols can be
downloaded free of charge at www.qiagen.com/qiacubeprotocols.

QIAamp Fast DNA Stool Mini Handbook 02/2020 11

QIAcube Connect.

12 QIAamp Fast DNA Stool Mini Handbook 02/2020

Equipment and Reagents to Be Supplied by User
When working with chemicals, always wear a suitable lab coat, disposable gloves and
protective goggles. For more information, consult the appropriate safety data sheets (SDSs),
available from the product supplier.

 Ethanol (96–100%)*
 1.5 ml and 2 ml microcentrifuge tubes (e.g., Eppendorf Safe-Lock, cat. no.
0030120.094 or Sarstedt Safe-Seal, cat. no. 72.695)†
 Pipet tips (pipet tips with aerosol barriers for preventing cross-contamination are
recommended)
 Microcentrifuge (with rotor for 2 ml tubes)
 Thermomixer with 2ml inlays or a water bath for incubation at 70°C
 Spatula (e.g., Sarstedt cat. no. 81.970)†
 Vortexer
 Ice

Using stool tubes

Stool tubes (for easier measurement of stool samples, stool tubes with integrated measuring
spoons in their lids are available, e.g., Sarstedt cat. no. 80.734 or Böttger cat. no.
07.023.2007 and 07.033.2007).

* Do not use denatured alcohol, which contains other substances, such as methanol or methylethylketone.
†
This is not a complete list of suppliers and does not include many important vendors of biological supplies.

QIAamp Fast DNA Stool Mini Handbook 02/2020 13

Important Notes
Preparation of reagents

InhibitEX Buffer (store at room temperature, 15–25°C)

Mix InhibitEX Buffer thoroughly by shaking. If a precipitate has formed, incubate at 37–70°C
until it has fully dissolved.

InhibitEX Buffer will exhibit a color change (orange) during storage; this does not affect the
functionality of the buffer.

Buffer AL* (store at room temperature)

Mix Buffer AL thoroughly by shaking before use. If a precipitate has formed, incubate at 70°C
until it has fully dissolved.

Buffer AL is stable for 1 year when stored closed at room temperature.

Note: Do not add proteinase K directly to Buffer AL.

Buffer AW1* (store at room temperature)

Buffer AW1 is supplied as a concentrate. Before using for the first time, add 25 ml ethanol
(96–100%) as indicated on the bottle.

Buffer AW1 is stable for 1 year when stored closed at room temperature.

Buffer AW1 should be thoroughly mixed before use.

* Contains chaotropic salt. Take appropriate laboratory safety measure and wear gloves when handling. Not
compatible with disinfecting agents that contain bleach. See page 7 for safety information.

14 QIAamp Fast DNA Stool Mini Handbook 02/2020

Buffer AW2* (store at room temperature)

Buffer AW2 is supplied as a concentrate. Before using for the first time, add 30 ml ethanol
(96–100%) to Buffer AW2 concentrate as indicated on the bottle.

Buffer AW2 is stable for 1 year when stored closed at room temperature.

Buffer AW2 should be thoroughly mixed before use.

* Contains sodium azide.

QIAamp Fast DNA Stool Mini Handbook 02/2020 15

Handling of QIAamp Mini spin columns

Owing to the sensitivity of nucleic acid amplification technologies, the following precautions
are necessary when handling QIAamp Mini spin columns to avoid cross-contamination
between samples.

 Carefully apply the sample or solution to the QIAamp Mini spin column. Pipet the sample
into the QIAamp Mini spin column without moistening the rim of the column.
 Change pipet tips between all liquid transfers. The use of aerosol-barrier pipet tips is
recommended.
 Avoid touching the QIAamp membrane with the pipet tip.
 To avoid cross-contamination, we recommend briefly centrifuging the microcentrifuge
tubes after each vortexing step to remove drops from the inside of the lid.
 Wear gloves throughout the entire procedure. In case of contact between gloves and
sample, change gloves immediately.
 Close the QIAamp Mini spin column before placing it in the microcentrifuge. Centrifuge
as described.
 Remove the QIAamp Mini spin column and collection tube from the microcentrifuge.
Place the QIAamp Mini spin column in a new collection tube. Discard the filtrate and the
collection tube. Please note that the filtrate may contain hazardous substances and
should be disposed of appropriately.
 Open only one QIAamp Mini spin column at a time, and take care to avoid generating
aerosols.
 For efficient parallel processing of multiple samples, fill a rack with collection tubes to
which the QIAamp Mini spin columns can be transferred after centrifugation. Discard
used collection tubes containing the filtrate and place the new collection tubes containing
the QIAamp Mini spin columns directly into the microcentrifuge.

16 QIAamp Fast DNA Stool Mini Handbook 02/2020

Centrifugation

QIAamp Mini spin columns fit into most standard 1.5 and 2 ml microcentrifuge tubes.
Additional 2 ml collection tubes are available separately.

All centrifugation steps should be carried out at room temperature (15–25°C) at 20,000 x g
(approximately 14,000 rpm). Increase the centrifugation time proportionately if your
centrifuge cannot provide 20,000 x g (e.g., instead of centrifuging for 5 minutes at
20,000 x g, centrifuge for 10 minutes at 10,000 x g).

QIAamp Fast DNA Stool Mini Handbook 02/2020 17

Protocol: Isolation of DNA from Stool for Human
DNA Analysis
Lysis conditions in this protocol are optimized to increase the ratio of human DNA to nonhuman
DNA. Nonhuman DNA is not excluded by this protocol.

Important points before starting

 All centrifugation steps should be carried out at room temperature (15–25°C) at

20,000 x g (approximately 14,000 rpm). Increase the centrifugation time
proportionately if your centrifuge cannot provide 20,000 x g (e.g., instead of
centrifuging for 5 min at 20,000 x g, centrifuge for 10 min at 10,000 x g).

Things to do before starting

 Prepare a thermomixer with 2 ml inlays or a water bath at 70°C for use in step 7
 Redissolve any precipitates in Buffer AL and InhibitEX Buffer by incubating at 37–70°C
 Add ethanol to Buffer AW1 and Buffer AW2 concentrates
 Mix all buffers before use

Procedure

1. Weigh 180–220 mg stool in a 2 ml microcentrifuge tube (not provided) and place tube
on ice.
This protocol is optimized for use with 180–220 mg stool but can also be used with
smaller amounts. There is no need to reduce the amounts of buffers when using smaller
amounts of stool. For samples >220 mg, see “Protocol: Isolation of DNA from Larger
Volumes of Stool”, page 30.
If the sample is liquid, pipet 200 µl into the microcentrifuge tube. Cut the end of the pipet
tip to make pipetting easier.

18 QIAamp Fast DNA Stool Mini Handbook 02/2020

 If the sample is frozen, use a scalpel or spatula to scrape bits of stool into a 2 ml
microcentrifuge tube on ice.
Note: When using frozen stool samples, take care that the samples do not thaw until
InhibitEX Buffer is added in step 2 to lyse the sample; otherwise the DNA in the sample
may degrade. After addition of InhibitEX Buffer, all following steps can be performed at
room temperature (15–25°C).
2. Add 1 ml InhibitEX Buffer to each stool sample. Vortex continuously for 1 min or until the
stool sample is thoroughly homogenized.
Note: It is important to vortex the samples thoroughly. This helps ensure maximum DNA
concentration in the final eluate.
3. Centrifuge sample at full speed for 1 min to pellet stool particles.
IMPORTANT: Do not transfer any solid material. If particles are still visible in the
supernatant, centrifuge the sample again.
4. Pipet 25 µl proteinase K into a new 2 ml microcentrifuge tube (not provided).
5. Pipet 600 µl supernatant from step 3 into the 2 ml microcentrifuge tube containing
proteinase K.
6. Add 600 µl Buffer AL and vortex for 15 s.
Note: Do not add proteinase K directly to Buffer AL. It is essential that the sample and
Buffer AL are thoroughly mixed to form a homogeneous solution.
7. Incubate at 70°C for 10 min.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).
8. Add 600 µl of ethanol (96–100%) to the lysate, and mix by vortexing.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).
9. Carefully apply 600 µl lysate from step 8 to the QIAamp spin column. Close the cap and
centrifuge at full speed for 1 min. Place the QIAamp spin column in a new 2 ml
collection tube, and discard the tube containing the filtrate.

QIAamp Fast DNA Stool Mini Handbook 02/2020 19

10. Repeat step 9 until all of the lysate has been loaded on the column.
Close each spin column to avoid aerosol formation during centrifugation. If the lysate has
not completely passed through the column after centrifugation, centrifuge again until the
QIAamp spin column is empty.
11. Carefully open the QIAamp spin column and add 500 µl Buffer AW1. Centrifuge at full
speed for 1 min. Place the QIAamp spin column in a new 2 ml collection tube, and
discard the collection tube containing the filtrate.
12. Carefully open the QIAamp spin column and add 500 µl Buffer AW2. Centrifuge at full
speed for 3 min. Discard the collection tube containing the filtrate.
Note: Residual Buffer AW2 in the eluate may cause problems in downstream
applications. Some centrifuge rotors may vibrate upon deceleration, causing the flow-
through containing Buffer AW2 to come in contact with the QIAamp spin column.
Removing the QIAamp spin column and collection tube from the rotor may also cause
flow-through to come in contact with the QIAamp spin column.
13. Place the QIAamp spin column in a new 2 ml collection tube (not provided) and discard
the old collection tube with the filtrate. Centrifuge at full speed for 3 min.
This step helps to eliminate the chance of possible Buffer AW2 carryover.
14. Transfer the QIAamp spin column into a new, labeled 1.5 ml microcentrifuge tube (not
provided) and pipet 200 µl Buffer ATE directly onto the QIAamp membrane. Incubate for
1 min at room temperature, then centrifuge at full speed for 1 min to elute DNA.
If yield is to be quantified by UV absorbance, blank the measuring device using Buffer
ATE to avoid false results. For more information about elution and how to determine
DNA yield, purity and length, see the Appendix on 37. For long-term storage, we
recommend keeping the eluate at –30°C to –15°C.

20 QIAamp Fast DNA Stool Mini Handbook 02/2020

Protocol: Using Stool Tubes for Isolation of DNA
from Stool for Human DNA Analysis
For easier measurement of stool samples, stool tubes with integrated measuring
spoons in their lids are available. The spoons in these tubes are designed to collect
200 mg stool. This protocol is optimized for usage with such stool tubes.

Lysis conditions in this protocol are optimized to increase the ratio of human DNA to nonhuman
DNA. Nonhuman DNA is not excluded by this protocol.

Important points before starting

 All centrifugation steps should be carried out at room temperature (15–25°C) at

20,000 x g (approximately 14,000 rpm). Increase the centrifugation time
proportionately if your centrifuge cannot provide 20,000 x g (e.g., instead of
centrifuging for 5 min at 20,000 x g, centrifuge for 10 min at 10,000 x g).

Things to do before starting

 Prepare a thermomixer with 2 ml inlays or a water bath at 70°C for use in step 8
 Redissolve any precipitates in Buffer AL and InhibitEX Buffer by incubating at 37–70°C.
 Add ethanol to Buffer AW1 and Buffer AW2 concentrates
 Mix all buffers before use

Procedure

1. Use the spoon integrated in the cap of a stool tube (not provided) to measure 180–220 mg
of the stool sample. A level spoonful will correspond to approximately 200 mg stool.
Close the tube and place it on ice.
A spatula should be used to remove excess stool from the spoon.

QIAamp Fast DNA Stool Mini Handbook 02/2020 21

2. Add 2 ml InhibitEX Buffer to each stool sample. Vortex continuously for 1 min or until the
stool sample is thoroughly homogenized.
Note: It is important to vortex the samples thoroughly. This helps ensure maximum DNA
concentration in the final eluate. After InhibitEX Buffer has been added, all the following
steps can be carried out at room temperature (15–25°C).
3. Pipet 1.2 ml of the stool lysate into a labeled 2 ml microcentrifuge tube (not provided).
Cut the ends off the pipet tips to make pipetting viscous samples easier.
4. Centrifuge sample at full speed for 1 min to pellet stool particles.
IMPORTANT: Do not transfer any solid material. If particles are still visible in the
supernatant, centrifuge the sample again.
5. Pipet 25 µl proteinase K into a new 2 ml microcentrifuge tube (not provided).
6. Pipet 600 µl supernatant from step 4 into the 2 ml microcentrifuge tube containing
proteinase K.
7. Add 600 µl Buffer AL and vortex for 15 s.
Note: Do not add proteinase K directly to Buffer AL. It is essential that the sample and
Buffer AL are thoroughly mixed to form a homogeneous solution.
8. Incubate at 70°C for 10 min.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).
9. Add 600 µl of ethanol (96–100%) to the lysate, and mix by vortexing.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).
10. Carefully apply 600 µl lysate from step 9 to the QIAamp spin column. Close the cap and
centrifuge at full speed for 1 min. Place the QIAamp spin column in a new 2 ml
collection tube, and discard the tube containing the filtrate.
11. Repeat step 10 until all of the lysate has been loaded on the column.
Close each spin column to avoid aerosol formation during centrifugation. If the lysate has
not completely passed through the column after centrifugation, centrifuge again until the
QIAamp spin column is empty.

22 QIAamp Fast DNA Stool Mini Handbook 02/2020

12. Carefully open the QIAamp spin column and add 500 µl Buffer AW1. Centrifuge at full
speed for 1 min. Place the QIAamp spin column in a new 2 ml collection tube, and
discard the collection tube containing the filtrate.
13. Carefully open the QIAamp spin column and add 500 µl Buffer AW2. Centrifuge at full
speed for 3 min. Discard the collection tube containing the filtrate.
Note: Residual Buffer AW2 in the eluate may cause problems in downstream
applications. Some centrifuge rotors may vibrate upon deceleration, causing the flow-
through containing Buffer AW2 to come in contact with the QIAamp spin column.
Removing the QIAamp spin column and collection tube from the rotor may also cause
flow-through to come into contact with the QIAamp spin column.
14. Place the QIAamp spin column in a new 2 ml collection tube (not provided) and discard
the old collection tube with the filtrate. Centrifuge at full speed for 3 min.
This step helps to eliminate the chance of possible Buffer AW2 carryover.
15. Transfer the QIAamp spin column into a new, labeled 1.5 ml microcentrifuge tube (not
provided) and pipet 200 µl Buffer ATE directly onto the QIAamp membrane. Incubate for
1 min at room temperature, then centrifuge at full speed for 1 min to elute DNA.
If yield is to be quantified by UV absorbance, blank the measuring device using Buffer
ATE to avoid false results. For more information about elution and how to determine
DNA yield, purity and length, see the Appendix on page 37. For long-term storage, we
recommend keeping the eluate at –30°C to –15°C.

QIAamp Fast DNA Stool Mini Handbook 02/2020 23

Protocol: Isolation of DNA from Stool for
Pathogen Detection
Lysis conditions in this protocol are optimized to increase the ratio of nonhuman DNA to human
DNA. Human DNA is not excluded by this protocol.

Important points before starting

 For detection of cells that are difficult to lyse, such as Gram-positive bacteria, the lysis
temperature in step 3 can be increased to 95°C, if necessary.
 All centrifugation steps should be carried out at room temperature (15–25°C) at
20,000 x g (approximately 14,000 rpm). Increase the centrifugation time
proportionately if your centrifuge cannot provide 20,000 x g (e.g., instead of
centrifuging for 5 min at 20,000 x g, centrifuge for 10 min at 10,000 x g).

Things to do before starting

 Prepare a thermomixer with 2 ml inlays or a water bath at 70°C for use in steps 3 and 8.
 Redissolve any precipitates in Buffer AL and InhibitEX Buffer by incubating at 37–70°C.
 Add ethanol to Buffer AW1 and Buffer AW2 concentrates.
 Mix all buffers before use.

Procedure
100 mg
1. Weigh 180–220 mg stool in a 2 ml microcentrifuge tube (not provided) and place tube
on ice.
This protocol is optimized for use with 180–220 mg stool but can also be used with
smaller amounts. There is no need to reduce the amounts of buffers when using smaller
amounts of stool. For samples >220 mg, see “Protocol: Isolation of DNA from Larger
Volumes of Stool”, page 30.

24 QIAamp Fast DNA Stool Mini Handbook 02/2020

 If the sample is liquid, pipet 200 µl into the microcentrifuge tube. Cut the end of the pipet
tip to make pipetting easier.
If the sample is frozen, use a scalpel or spatula to scrape bits of stool into a 2 ml
microcentrifuge tube on ice.
Note: When using frozen stool samples, take care that the samples do not thaw until
InhibitEX Buffer is added in step 2 to lyse the sample; otherwise the DNA in the sample
may degrade. After addition of InhibitEX Buffer, all following steps can be performed at
room temperature (15–25°C).
2. Add 1 ml InhibitEX Buffer to each stool sample. Vortex continuously for 1 min or until the
stool sample is thoroughly homogenized.
Note: It is important to vortex the samples thoroughly. This helps ensure maximum DNA
concentration in the final eluate.
3. Heat the suspension for 5 min at 70°C. Vortex for 15 s.
This heating step helps to lyse bacteria and other parasites. The lysis temperature can be
increased to 95°C for cells that are difficult to lyse (such as Gram-positive bacteria).
4. Centrifuge sample at full speed for 1 min to pellet stool particles.
IMPORTANT: Do not transfer any solid material. If particles are still visible in the
supernatant, centrifuge the sample again.
5. Pipet 15 µl proteinase K into a new 1.5 ml microcentrifuge tube (not provided).
6. Pipet 200 µl supernatant from step 4 into the 1.5 ml microcentrifuge tube containing
proteinase K.
7. Add 200 µl Buffer AL and vortex for 15 s.
Note: Do not add proteinase K directly to Buffer AL. It is essential that the sample and
Buffer AL are thoroughly mixed to form a homogeneous solution.
8. Incubate at 70°C for 10 min.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).
9. Add 200 µl of ethanol (96–100%) to the lysate, and mix by vortexing.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).

QIAamp Fast DNA Stool Mini Handbook 02/2020 25

10. Carefully apply 600 µl lysate from step 9 to the QIAamp spin column. Close the cap and
centrifuge at full speed for 1 min. Place the QIAamp spin column in a new 2 ml
collection tube, and discard the tube containing the filtrate.
Close each spin column to avoid aerosol formation during centrifugation. If the lysate has
not completely passed through the column after centrifugation, centrifuge again until the
QIAamp spin column is empty.
11. Carefully open the QIAamp spin column and add 500 µl Buffer AW1. Centrifuge at full
speed for 1 min. Place the QIAamp spin column in a new 2 ml collection tube, and
discard the collection tube containing the filtrate.
12. Carefully open the QIAamp spin column and add 500 µl Buffer AW2. Centrifuge at full
speed for 3 min. Discard the collection tube containing the filtrate.
Note: Residual Buffer AW2 in the eluate may cause problems in downstream
applications. Some centrifuge rotors may vibrate upon deceleration, causing the flow-
through containing Buffer AW2 to come in contact with the QIAamp spin column.
Removing the QIAamp spin column and collection tube from the rotor may also cause
flow-through to come into contact with the QIAamp spin column.
13. Place the QIAamp spin column in a new 2 ml collection tube (not provided) and discard
the old collection tube with the filtrate. Centrifuge at full speed for 3 min.
This step helps to eliminate the chance of possible Buffer AW2 carryover.
14. Transfer the QIAamp spin column into a new, labeled 1.5 ml microcentrifuge tube (not
provided) and pipet 200 µl Buffer ATE directly onto the QIAamp membrane. Incubate for
1 min at room temperature, then centrifuge at full speed for 1 min to elute DNA.
If yield is to be quantified by UV absorbance, blank the measuring device using Buffer
ATE to avoid false results. For more information about elution and how to determine
DNA yield, purity and length, see the Appendix on page 37. For long-term storage, we
recommend keeping the eluate at –30°C to –15°C.

26 QIAamp Fast DNA Stool Mini Handbook 02/2020

Protocol: Using Stool Tubes for Isolation of DNA
from Stool for Pathogen Detection
For easier measurement of stool samples, stool tubes with integrated measuring spoons in their
lids are available. The spoons in these tubes are designed to collect 200 mg stool. This protocol
is optimized for usage with such stool tubes.

Lysis conditions in this protocol are optimized to increase the ratio of nonhuman DNA to human
DNA. Human DNA is not excluded by this procedure.

Important points before starting

 For detection of cells that are difficult to lyse, such as Gram-positive bacteria, the lysis
temperature in step 3 can be increased to 95°C, if necessary.
 All centrifugation steps should be carried out at room temperature (15–25°C) at
20,000 x g (approximately 14,000 rpm). Increase the centrifugation time
proportionately if your centrifuge cannot provide 20,000 x g (e.g., instead of
centrifuging for 5 min at 20,000 x g, centrifuge for 10 min at 10,000 x g).

Things to do before starting

 Prepare a thermomixer with 2 ml inlays or a water bath at 70°C for use in steps 4 and
9.
 Redissolve any precipitates in Buffer AL and InhibitEX Buffer by incubating at 37–70°C.
 Add ethanol to Buffer AW1 and Buffer AW2 concentrates.
 Mix all buffers before use.

QIAamp Fast DNA Stool Mini Handbook 02/2020 27

Procedure

1. Use the spoon integrated in the cap of a stool tube (not provided) to measure 180–220 mg
of the stool sample. A level spoonful will correspond to approximately 200 mg stool.
Close the tube and place it on ice.
A spatula should be used to remove excess stool from the spoon.
2. Add 2 ml InhibitEX Buffer to each stool sample. Vortex continuously for 1 min or until the
stool sample is thoroughly homogenized.
Note: It is important to vortex the samples thoroughly. This helps ensure maximum DNA
concentration in the final eluate. After InhibitEX Buffer has been added, all the following
steps can be carried out at room temperature (15–25°C).
3. Pipet 1.2 ml of the stool lysate into a labeled 2 ml microcentrifuge tube (not provided).
Cut the ends off the pipet tips to make pipetting viscous samples easier.
4. Heat the suspension for 5 min at 70°C. Vortex for 15 s.
This heating step helps to lyse bacteria and other parasites. The lysis temperature can be
increased to 95°C for cells that are difficult to lyse (such as Gram-positive bacteria).
5. Centrifuge sample at full speed for 1 min to pellet stool particles.
IMPORTANT: Do not transfer any solid material. If particles are still visible in the
supernatant, centrifuge the sample again.
6. Pipet 15 µl proteinase K into a new 1.5 ml microcentrifuge tube (not provided).
7. Pipet 200 µl supernatant from step 5 into the 1.5 ml microcentrifuge tube containing
proteinase K.
8. Add 200 µl Buffer AL and vortex for 15 s.
Note: Do not add proteinase K directly to Buffer AL. It is essential that the sample and
Buffer AL are thoroughly mixed to form a homogeneous solution.
9. Incubate at 70°C for 10 min.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).

28 QIAamp Fast DNA Stool Mini Handbook 02/2020

10. Add 200 µl of ethanol (96–100%) to the lysate, and mix by vortexing.
Centrifuge briefly to remove drops from the inside of the tube lid (optional).
11. Carefully apply 600 µl lysate from step 10 to the QIAamp spin column. Close the cap
and centrifuge at full speed for 1 min. Place the QIAamp spin column in a new 2 ml
collection tube, and discard the tube containing the filtrate.
Close each spin column to avoid aerosol formation during centrifugation. If the lysate has
not completely passed through the column after centrifugation, centrifuge again until the
QIAamp spin column is empty.
12. Carefully open the QIAamp spin column and add 500 µl Buffer AW1. Centrifuge at full
speed for 1 min. Place the QIAamp spin column in a new 2 ml collection tube, and
discard the collection tube containing the filtrate.
13. Carefully open the QIAamp spin column and add 500 µl Buffer AW2. Centrifuge at full
speed for 3 min. Discard the collection tube containing the filtrate.
Note: Residual Buffer AW2 in the eluate may cause problems in downstream
applications. Some centrifuge rotors may vibrate upon deceleration, causing the flow-
through containing Buffer AW2 to come in contact with the QIAamp spin column.
Removing the QIAamp spin column and collection tube from the rotor may also cause
flow-through to come in contact with the QIAamp spin column.
14. Place the QIAamp spin column in a new 2 ml collection tube (not provided) and discard
the old collection tube with the filtrate. Centrifuge at full speed for 3 min.
This step helps to eliminate the chance of possible Buffer AW2 carryover.
15. Transfer the QIAamp spin column into a new, labeled 1.5 ml microcentrifuge tube (not
provided) and pipet 200 µl Buffer ATE directly onto the QIAamp membrane. Incubate for
1 min at room temperature, then centrifuge at full speed for 1 min to elute DNA.
If yield is to be quantified by UV absorbance, blank the measuring device using Buffer
ATE to avoid false results. For more information about elution and how to determine
DNA yield, purity and length, see the Appendix on page 37. For long-term storage, we
recommend keeping the eluate at –30°C to –15°C.

QIAamp Fast DNA Stool Mini Handbook 02/2020 29

Protocol: Isolation of DNA from Larger Volumes
of Stool
This protocol is recommended when the target DNA is not distributed homogeneously in the
stool. Using a relatively large amount of starting material enhances the chances of isolating
DNA from low-titer sources in stool samples. Note that the yield and concentration of DNA
isolated using this protocol are not greater than the yield and concentration of DNA isolated
using the other protocols in this handbook.

Important points before starting

 Ensure that Buffers AW1 and AW2 have been prepared according to the instructions on
the labels.
 Mix all buffers before use.
 If a precipitate has formed in InhibitEx Buffer or AL, dissolve by incubating at 37–70°C.
 All centrifugation steps should be carried out at room temperature (15–25°C) at
20,000 x g (approximately 14,000 rpm). Increase the centrifugation time
proportionately if your centrifuge cannot provide 20,000 x g (e.g., instead of
centrifuging for 5 min at 20,000 x g, centrifuge for 10 min at 10,000 x g).

Procedure

1. Weigh the stool sample and add 10 volumes of InhibitEx Buffer (e.g., add 10 ml
InhibitEx buffer to 1 g stool).
If necessary, additional InhibitEx Buffer can be purchased separately (see Ordering
Information on page Error! Bookmark not defined.).
2. Vortex vigorously for 1 min or until the stool sample is thoroughly homogenized.
Note: It is important to vortex the samples thoroughly. This helps ensure maximum DNA
concentration in the final eluate.

30 QIAamp Fast DNA Stool Mini Handbook 02/2020

3. Pipet 2 ml of lysate into a labeled 2 ml microcentrifuge tube (not provided).
Cut the ends off the pipet tips to make pipetting viscous samples easier.
4. Depending on the downstream application, continue with step 4 of “Protocol using Stool
Tubes for Isolation of DNA from Stool for Human DNA Analysis” (page 21) or step 4 of
“Protocol using Stool Tubes for Isolation of DNA from Stool for Pathogen Detection”
(page 27).

QIAamp Fast DNA Stool Mini Handbook 02/2020 31

Troubleshooting Guide
This troubleshooting guide may be helpful in solving any problems that may arise. For more
information, see also the Frequently Asked Questions page at our Technical Support Center:
www.qiagen.com/FAQ/FAQList.aspx. The scientists in QIAGEN Technical Services are
always happy to answer any questions you may have about either the information and
protocols in this handbook or sample and assay technologies (for contact information, see
back cover or visit www.qiagen.com).

Comments and suggestions

Little or no DNA in the eluate

a) Sample stored Samples should be stored at 2–8°C or –30°C to –15°C.

incorrectly

b) Insufficient Repeat the DNA purification procedure with a new sample.

homogenization of Be sure to mix the sample and InhibitEX Buffer until the
stool sample in sample is thoroughly homogenized.
InhibitEX Buffer

c) Insufficient mixing Repeat the DNA purification procedure with a new sample.
with Buffer AL Be sure to mix the sample and Buffer AL immediately and
thoroughly by pulse-vortexing.

d) No alcohol added Repeat the purification procedure with a new sample.

to the lysate before
loading onto the
QIAamp spin
column

e) Low-percentage Repeat the purification procedure with a new sample.

alcohol used
instead of 100%

32 QIAamp Fast DNA Stool Mini Handbook 02/2020

 Comments and suggestions

f) DNA not eluted To increase elution efficiency, pipet Buffer ATE onto the
efficiently QIAamp spin column and incubate the column for 5 min at
room temperature (15–25°C) before centrifugation.

g) Buffer AW1 or Check that Buffer AW1 and Buffer AW2 concentrates were
Buffer AW2 diluted with correct volumes of pure ethanol. Repeat the
prepared purification procedure with a new sample.
incorrectly

h) Buffer AW1 or Check that Buffer AW1 and Buffer AW2 concentrates were
Buffer AW2 diluted with 96–100% ethanol. Repeat the purification
prepared with 70% procedure with a new sample.
ethanol

i) Buffer AW1 and Ensure that Buffer AW1 and Buffer AW2 are used in the
Buffer AW2 used correct order in the protocol. Add 200 µl Buffer AL and
in the wrong order 200 µl ethanol to the eluate, and continue with step 10 of
“Protocol: Isolation of DNA from Stool for Pathogen
Detection“ (page 24).

A260/A280 ratio for purified nucleic acids is low

a) Too much DNA The QIAamp Fast DNA Stool Mini Kit purifies total DNA,
used in which could originate from many different organisms present
downstream in the original stool sample (e.g., human, animal, plant,
reaction bacterial). If the amount of total DNA is too high, PCR could
be inhibited by excess total DNA. Reduce the amount of
eluate used in the downstream reaction if possible.

b) Inefficient lysis of The amount of target DNA in the eluate may be low if the
target cells target cells are difficult to lyse, as is the case with some
bacteria and parasites. In future preparations, increase lysis
temperature to 95°C and/or incubation time as required.

c) Not enough DNA Check “Little or no DNA in the eluate” for possible reasons.
in eluate

QIAamp Fast DNA Stool Mini Handbook 02/2020 33

 Comments and suggestions

d) Inhibitory See “A260/A280 ratio for purified nucleic acids is low” for
substances in possible reasons. Bring the eluate volume to 200 µl if
preparation necessary, and repeat the purification procedure from step 5
of “Protocol: Isolation of DNA from Stool for Pathogen
Detection“ (page 28).

e) Residual Buffer Add 200 µl Buffer AL and 200 µl ethanol to the eluate, and
AW2 in the eluate continue with step 10 of “Protocol: Isolation of DNA from
Stool for Pathogen Detection“ (page 28).

f) Buffer AW1 and Ensure that Buffer AW1 and Buffer AW2 are used in the
Buffer AW2 used correct order in the protocol. Add 200 µl Buffer AL and
in the wrong order 200 µl ethanol to the eluate, and continue with step 10 of
“Protocol: Isolation of DNA from Stool for Pathogen
Detection“ (page 28).

A260/A280 ratio for purified nucleic acids is high

High level of Add 20 µl of RNase A (20 mg/ml) to the eluate and

residual RNA incubate for 10 minutes at room temperature (15–25°C).

DNA does not perform well in downstream applications

a) Too much DNA The QIAamp Fast DNA Stool Mini Kit purifies total DNA,
used in which could originate from many different organisms present
downstream in the original stool sample (e.g., human, animal, plant,
reaction bacterial). If the amount of total DNA is too high, PCR could
be inhibited by excess total DNA. Reduce the amount of
eluate used in the downstream reaction if possible.

b) Inefficient lysis of The amount of target DNA in the eluate may be low if the
target cells target cells are difficult to lyse, as is the case with some
bacteria and parasites. In future preparations, increase lysis
temperature to 95°C and/or incubation time as required.

c) Not enough DNA Check “Little or no DNA in the eluate” for possible reasons.
in eluate

34 QIAamp Fast DNA Stool Mini Handbook 02/2020

 Comments and suggestions

d) Inhibitory See “A260/A280 ratio for purified nucleic acids is low” for
substances in possible reasons. Bring the eluate volume to 200 µl if
preparation necessary, and repeat the purification procedure from step 5
of “Protocol: Isolation of DNA from Stool for Pathogen
Detection“ (page 28).

e) Residual Buffer Add 200 µl Buffer AL and 200 µl ethanol to the eluate, and
AW2 in the eluate continue with step 10 of “Protocol: Isolation of DNA from
Stool for Pathogen Detection“ (page 28).

f) Buffer AW1 and Ensure that Buffer AW1 and Buffer AW2 are used in the
Buffer AW2 used correct order in the protocol. Add 200 µl Buffer AL and 200
in the wrong order µl ethanol to the eluate, and continue with step 10 of
“Protocol: Isolation of DNA from Stool for Pathogen
Detection“ (page 28).

g) Insufficient mixing Repeat the purification with new samples.

with InhibitEX
Buffer

h) High level of Add 20 µl of RNase A (20 mg/ml) to the eluate and

residual RNA incubate for 10 min at room temperature (15–25°C). Add
200 µl Buffer AL and 200 µl ethanol to the eluate, and
continue with step 10 of “Protocol: Isolation of DNA from
Stool for Pathogen Detection“ (page 28).

i) Reduced sensitivity Determine the maximum volume of eluate suitable for your
of amplification amplification reaction. Reduce or increase the volume of
reaction eluate added to the reaction.

Little or no supernatant visible after initial centrifugation step

Insufficient Increase the centrifugation time proportionately if your

centrifugal force centrifuge cannot provide 20,000 x g (e.g., instead of
centrifuging for 5 minutes at 20,000 x g, centrifuge for
10 minutes at 10,000 x g).

QIAamp Fast DNA Stool Mini Handbook 02/2020 35

 Comments and suggestions

White precipitate in InhibitEX Buffer or Buffer AL

a) Storage at low Any precipitate in InhibitEX Buffer or Buffer AL must be

temperature or dissolved by incubating the buffer at 37–70°C.
prolonged storage

Precipitate after addition of Buffer AL or ethanol

a) A precipitate may In most cases, the precipitate will dissolve during incubation
form upon addition at 70°C. The precipitates do not interfere with the QIAamp
of Buffer AL procedure or with any subsequent application.

b) A precipitate may In most cases, the precipitate will dissolve after vortexing
form upon addition immediately following its appearance. The precipitates do
of ethanol not interfere with the QIAamp procedure or with any
subsequent application.

General handling

a) Lysate not Centrifuge for 1 min at full speed or until all the lysate has
completely passed passed through the membrane.
through silica
membrane

b) Cross- To avoid cross-contamination when handling QIAamp spin

contamination columns, read “Handling of QIAamp Mini spin columns” on
between samples page 16. Repeat the purification procedure with new
samples.

36 QIAamp Fast DNA Stool Mini Handbook 02/2020

Appendix: Determination of Concentration,
Yield, Purity and Length of DNA
Determination of concentration, yield and purity

DNA yields are determined from the concentration of DNA in the eluate, measured by
absorbance at 260 nm. Purity is determined by calculating the ratio of absorbance at 260 nm
to absorbance at 280 nm. Pure DNA has an A260/A280 ratio of 1.7–1.9. Absorbance readings
at 260 nm should lie between 0.1 and 1.0 to be accurate.

Sample dilution should be adjusted accordingly. Use elution buffer or water (as appropriate)
to dilute samples and to calibrate the spectrophotometer. Measure the absorbance at 260 and
280 nm, or scan absorbance from 220–320 nm (a scan will show if there are other factors
affecting absorbance at 260 nm). Both DNA and RNA are measured with a
spectrophotometer. To measure only DNA, a fluorometer must be used.

QIAamp Fast DNA Stool Mini Handbook 02/2020 37

Determination of DNA length

The length of genomic DNA can be determined by pulsed-field gel electrophoresis (PFGE)
through an agarose gel. The DNA should be concentrated by alcohol* precipitation and
reconstituted by gentle agitation in approximately 30 µl TE buffer, pH 8.0,* for at least
30 minutes at 60°C. Avoid drying the DNA pellet for more than 10 min at room temperature
(15–25°C) since over-dried genomic DNA is very difficult to redissolve. Load 3–5 µg DNA per
well. Standard PFGE conditions are as follows:

 1% agarose gel in 0.5x TBE electrophoresis buffer*

 Switch intervals: 5–40 s
 Run time: 17 h
 Voltage: 170 V

* When working with chemicals, always wear a suitable lab coat, disposable gloves and protective goggles. For more
information, consult the appropriate material safety data sheets (MSDSs), available from the product supplier.

38 QIAamp Fast DNA Stool Mini Handbook 02/2020

Ordering Information

Product Contents Cat. no.

QIAamp Fast DNA Stool 50 QIAamp Mini Spin Columns, 51604

Mini Kit (50) Proteinase K, InhibitEX Buffer, wash and
elution buffers, Collection Tubes (2ml)

Related Products

QIAamp DNA Mini Kit — for genomic DNA purification from tissue,
blood and body fluids

QIAamp DNA Mini Kit (50)* 50 QIAamp Mini Spin Columns, 51304
Proteinase K, Reagents, Buffers,
Collection Tubes (2 ml)

QIAamp DNA Mini Kit 250 QIAamp Mini Spin Columns, 51306
(250)* Proteinase K, Reagents, Buffers,
Collection Tubes (2 ml)

QIAamp DNA Blood Mini Kit — for genomic DNA purification from blood
and body fluids

QIAamp DNA Blood Mini Kit 50 QIAamp Mini Spin Columns, 51104
(50)* QIAGEN Protease, Reagents, Buffers,
Collection Tubes (2 ml)

QIAamp DNA Blood Mini Kit 250 QIAamp Mini Spin Columns, 51106
(250)* QIAGEN Protease, Reagents, Buffers,
Collection Tubes (2 ml)

QIAamp Fast DNA Stool Mini Handbook 02/2020 39

Product Contents Cat. no.
QIAamp 96 DNA Blood Mini Kit — for high-throughput genomic DNA
purification from blood and body fluids

QIAamp 96 DNA Blood Kit 4 QIAamp 96 Plates, QIAGEN 51161

(4) Protease, Reagents, Buffers, Lysis Blocks,
Tape Pads, Collection Vessels

QIAamp 96 DNA Blood Kit 12 QIAamp 96 Plates, QIAGEN 51162

(12) Protease, Reagents, Buffers, Lysis Blocks,
Tape Pads, Collection Vessels

QIAamp RNA Blood Mini Kit — for total RNA purification from blood and
body fluids

QIAamp RNA Blood Mini Kit 20 QIAamp Mini Spin Columns, 20 52304
(50)* QIAshredder Spin Columns, Collection
Tubes (1.5 ml and 2 ml), RNase-Free
Reagents and Buffers

QIAamp Viral RNA Mini Kit — for viral RNA purification from plasma,
serum and body fluids

QIAamp Viral RNA Mini Kit 50 QIAamp Mini Spin Columns, Carrier 52904
(50)* RNA, Collection Tubes (2 ml), RNase-
Free Buffers

QIAamp Viral RNA Mini Kit 250 QIAamp Mini Spin Columns, 52906
(250)* Carrier RNA, Collection Tubes (2 ml),
RNase-Free Buffers

40 QIAamp Fast DNA Stool Mini Handbook 02/2020

Product Contents Cat. no.
QIAcube Connect — for fully automated nucleic acid extraction with
QIAGEN spin-column kits

QIAcube Connect* Instrument, connectivity package, 1-year

Inquire
warranty on parts and labor

Starter Pack, QIAcube Filter-tips, 200 µl (1024), 1000 µl filter-

tips (1024), 30 ml reagent bottles (12), 990395
rotor adapters (240), elution tubes
(240), rotor adapter holder
* All QIAcube Connect instruments are provided with a region-specific connectivity package, including tablet and
equipment necessary to connect to the local network. Further, QIAGEN offers comprehensive instrument service
products, including service agreements, installation, introductory training and preventive subscription. Contact your
local sales representative to learn about your options.

For up-to-date licensing information and product-specific disclaimers, see the respective
QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are
available at www.qiagen.com or can be requested from QIAGEN Technical Services or your
local distributor.

Document Revision History

Date Changes

February 2020 Updated text, ordering information and intended use for QIAcube Connect.

QIAamp Fast DNA Stool Mini Handbook 02/2020 41

Limited License Agreement for QIAamp Fast DNA Stool Mini Kit
Use of this product signifies the agreement of any purchaser or user of the product to the following terms:
1. The product may be used solely in accordance with the protocols provided with the product and this handbook and for use with components contained in the kit
only. QIAGEN grants no license under any of its intellectual property to use or incorporate the enclosed components of this kit with any components not included
within this kit except as described in the protocols provided with the product, this handbook, and additional protocols available at www.qiagen.com. Some of
these additional protocols have been provided by QIAGEN users for QIAGEN users. These protocols have not been thoroughly tested or optimized by
QIAGEN. QIAGEN neither guarantees them nor warrants that they do not infringe the rights of third-parties.
2. Other than expressly stated licenses, QIAGEN makes no warranty that this kit and/or its use(s) do not infringe the rights of third-parties.
3. This kit and its components are licensed for one-time use and may not be reused, refurbished, or resold.
4. QIAGEN specifically disclaims any other licenses, expressed or implied other than those expressly stated.
5. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN
may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any
action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.
For updated license terms, see www.qiagen.com.

Trademarks: QIAGEN®, Sample to Insight®, QIAamp®, QIAcube®, InhibitEX® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when
not specifically marked as such, are not to be considered unprotected by law.

HB-1764-002 © 2020 QIAGEN, all rights reserved.

42 QIAamp Fast DNA Stool Mini Handbook 02/2020

Notes

QIAamp Fast DNA Stool Mini Handbook 02/2020 43

Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com

44
HB-1764-002 QIAamp Fast DNA Stool Mini Handbook 02/2020