MICROSCAN +

(Plate Reader)

User Manual Version 1.0

 Microscan + (Plate Reader) User Manual

Table of Contents

1. INSTALLATION ............................................................................................................................................................ 2

1.1. UNPACKING ..............................................................................................................................................................2

1.2. INSTALLATION ENVIRONMENT REQUIREMENTS .......................................................................................................2
1.2.1 Transportation and Storage ................................................................................................................................2
1.2.2. Operation Environment......................................................................................................................................2
1.3. CONNECTION ...........................................................................................................................................................2

2. FUNCTION ..................................................................................................................................................................... 4

2.1. INTRODUCTION..............................................................................................................................................................4
2.2. FRONT VIEW (FIGURE 1) ..............................................................................................................................................4
2.3. REAR VIEW (FIGURE 2) ................................................................................................................................................5

3. OPERATION .................................................................................................................................................................. 6

3.1. POWER-ON ..............................................................................................................................................................6

3.2. MAIN MENU..............................................................................................................................................................6
3.3. MEASURE .................................................................................................................................................................6
3.4. PROGRAM ................................................................................................................................................................8
3.4.1. Edit Test..........................................................................................................................................................9
3.4.2. New Test ...................................................................................................................................................... 13
3.4.3. Delete Test ................................................................................................................................................... 14
3.5. QUALITY CONTROL ..................................................................................................................................................... 14
3.6. SETUP ......................................................................................................................................................................... 16
3.6.1. Date and Time .................................................................................................................................................. 16
3.6.2. Vibrancy ............................................................................................................................................................. 16
3.6.3. Light Source ...................................................................................................................................................... 17
3.6.4. Printer ........................................................................................................................................................... 17
3.6.5. Filter .............................................................................................................................................................. 17
3.7. SERVICE................................................................................................................................................................. 18
3.7.1. Data Transfer ............................................................................................................................................... 18
3.7.2. Result Review ............................................................................................................................................. 18
3.7.3. Standard Review ......................................................................................................................................... 18
3.8. RAPID MEASURE.................................................................................................................................................... 19

4. MAINTENANCE ......................................................................................................................................................... 20

4.1. NOTICE................................................................................................................................................................... 20
4.2. MAINTENANCE ....................................................................................................................................................... 20

5. TECHNICAL SPECIFICATIONS.............................................................................................................................. 21

6. TROUBLESHOOTING ............................................................................................................................................... 22

7. WARNING .................................................................................................................................................................... 23

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 Microscan + (Plate Reader) User Manual

1. Installation

1.1. Unpacking

When you receive your Microplate Reader, carefully unpack the package and check the device. If

you see any damages, file a claim immediately with your local supplier.

Put away the packaging material for future use.

Check the Microplate Reader and accessories against the packing list. If you find anything missing

or unconformable, please contact your local supplier.

1.2. Installation Environment Requirements

1.2.1 Transportation and Storage

Ambient temperature (-20 - 55)

Relative humidity ≤93%

Atmospheric pressure (50 - 106) kPa

1.2.2. Operation Environment

The Microplate Reader should be free of dust, vibration, corrosive and flammable gas,

interference by strong current and magnetic field as well as direct sunlight. Moreover, enough

space should be kept behind the device for good ventilation.

Ambient temperature: 10 - 40

Relative humidity: ≤85%

Atmospheric pressure: 86 - 106kPa

Power requirements: AC 220/110V, 50/60Hz

1.3. Connection

Check if the voltage conforms to requirements of the device. Make sure the power socket is

grounded correctly; otherwise you should connect a single ground wire to the Grounding port on

the device.

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Connect the printer to the Printer port on the device through a data cable.

Insert one end of the power cord into the Power socket on the rear panel of the device and other

end to the power supply.

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 Microscan + (Plate Reader) User Manual

2. Function

2.1. Introduction

The Microplate Reader is an 8-channel optical measuring system, which can be used to measure

the density of vertical light. It is a specialized analyzer to hold MICROPLATEs and then take

readings and process data.

The Microplate Reader adopts the touch screen which is comfortable to operate.

With the advanced high speed microprocessor and man-machine conversation design on the

software, the Microplate Reader makes the operation simpler and more convenient, result

calculation quicker and waiting time of operator much shorter.

2.2. Top View (Figure 1)

Touch Screen Sample Compartment

Figure 1

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2.3. Rear View (Figure 2)

Printer Port Serial RS232 Port Power Socket Grounding

Figure 2

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3. Operation

3.1. Power-on

When you place the Power switch to ON ( | ), the device will begin self-check and then enter the Main Menu

screen (Figure 3) if everything is normal. After powering on the device, you should wait for 10 minutes to

preheat the device, so that each component and the lamp within the device become steady.

Jan.18,2005
Main Menu
18:18:18

Measure Program

Quality Control Setup

Service Rapid Measure

Figure 3

3.2. Main Menu

The Main Menu displays the function keys and current date and time. You can enter the submenu

screen by touching corresponding function key.

3.3. Measure

Touch the Measure button, the Select Test screen as shown in Figure 4 is displayed. Touch

Pgdn to display the tests of other pages circularly (42 tests in total, 3 pages). Touch the Exit

button to return to the Main Menu.

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Measure Select Test

01 HBsAg 02 HBsAb

03 HBcAg 04 HBcAb

05 06

07 08

09 10

11 12

Pgdn Exit

Figure 4 Select Test screen

After you select the tests, the Plate No. and Vibrancy screen (Figure 5) is displayed.

Plate No. 00

Vibrancy

Exit OK

Figure 5 Plate No. and Vibrancy screen

Touch Plate No. and enter the Plate No. in the Plate No. screen (Figure 6). As shown in figure 6, the

CLR button is to delete what you have entered. After entering, touch YES to confirm and return to

the Plate No. and Vibrancy screen. If you touch Exit , then the Plate No. is 00. Touch OK to begin

measuring and analyzing the MICROPLATE plate. If you need to mix the sample by vibrating the plate

before measuring, touch Vibrancy and then the OK button.

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Plate No. ____________

1 2

3 4

5 6

7 8

9 0

. -

YES CLR

Exit

Figure 6 Plate No. screen

3.4. Program

The Program screen includes Edit Test, New Test and Delete Test (Figure 7). Touch Exit to

return to the Main Menu.

Program

Edit Test

New Test

Delete Test

Exit

Figure 7 Program screen

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3.4.1. Edit Test

Touch Edit Test to select the test that you want to edit (Figure 4), then enter the Edit Test

screen (Figure 8).

Note: After setting, touch OK to save your settings.

Edit Test HBsAg

Prim. Wav. 405nm

Sec. Wav. 630nm

Plate Layout

Calculation Mode

Exit OK

Figure 8 Edit Test screen

Touch the Prim. Wav. button, a menu will then appear to ask you to select the wavelength.

Please select as instructed by the package insert.

NOTE: Don’t close all the filters; otherwise the alarm message “Lamp L” will appear.

Touch the Sec. Wav. button, a menu will then appear to ask you to select the wavelength.

Please select as instructed by the package insert.

In the Plate Layout screen (Figure 9-1), the right column is for selecting the well position

you want to set, use the and buttons to select different

well columns. The box on top right corner displays the current strip being set. Touch

Exit to return to the screen as shown in figure 8.

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Automatic A01 Blank 01

B01 Pos. Control 01

C01 Sample 01

D01 Sample 02

E01 Sample 03

F01 Sample 04

Exit G01 Sample 05

H01 Sample 07

Figure 9-1 Plate Layout screen

After selecting the well position, the screen as shown in figure 9-2 will be displayed. The left

column is for setting the filling type and filling No. of the selected well position (Sample: 1-96;

Standard: 1-10; Quality Control: 1-10; Neg./Pos. control: 1-12; Blank well: 1-12), use the

Pgdn button to view each No. of filling type. Touch the selected well position once more to

exit its filling, refer to A01 in figure 9-2.

Standard 01 A01 Blank 01

Quality Control 01 B01 Pos. Control 01

Neg. Control 01 C01 Sample 01

Pos. Control 01 D01 Sample 02

Blank 01 E01 Sample 03

Sample 01 F01 Sample 04

Pgdn G01 Sample 05

H01 Sample 07

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 Microscan + (Plate Reader) User Manual

Figure 9-2 Plate Layout Menu

You can set the sample position quickly by using the Automatic button as shown in

figure 9-1. Since this function sets No. only for the original sample wells or blank wells, you

should touch Delete All (Figure 10) to delete all settings of the well positions before

auto filling. Then enter the Blank, Standard and Quality Control, etc, touch Automatic

and select Hor. Strip or Ver. Strip and No. of wells that you want to repeat.

Hor. Strip

Ver. Strip

Delete All

Exit

Figure 10 Automatic screen

Touch the Calculation Mode button as shown in figure 8, then select a method in the right list

appeared.

1) Absorbance: Read the absorbance of the sample directly, no submenu is displayed.

2) Qual Calculation: You should set Qual Grouping, Cut-off, Neg. Range, Pos. Range, Validity

Confirm and so forth. Please make settings as instructed by the package insert.

a) Qual Grouping 1-4: You can divide the qualitative results into four groups (Negative-,

Positive+, Weak Positive-+ and Strong Positive ++) in accordance with the cut-off you

have set.

b) Cut-off 1-3: Enter values of a, b and c in the CutOff formula a*NC+b*PC+c.

Where,

NC - Absorbance of the negative control well

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 Microscan + (Plate Reader) User Manual

PC - Absorbance of the positive control well

c) Neg. Range: When the absorbance exceeds the required range, the device will calculate

automatically with the negative control limit you have set.

d) Pos. Range: When the absorbance exceeds the required range, the device will calculate

automatically with the positive control limit you have set.

e) Validity Confirm 1-3: Set the range of absorbance for blank wells, positive and negative

control wells. The device will not calculate results when the absorbance exceeds the

range.

3) Quant Calculation: Some parameters are added on the basis of qualitative settings, such as

STD Concentration, Unit, Reference, Calibration Rule and Factor. Please make settings as

instructed by the package insert.

a) STD Concentration: You can set the concentrations of up to 8 standards. When setting,

you should begin with 1 from low concentration to high concentration. If No. of standards

is less than 8, you must set the remaining concentrations to 0.

b) Unit: Enter the concentration unit of the selected test.

c) Reference H: Enter the upper limit for the selected test.

d) Reference L: Enter the lower limit for the selected test.

e) Calibration Rule (C - Concentration, A - Absorbance, k - Slope, b - Intercept)

 Factor

Formula: A =kC +b.

k and b should be entered by the operator manually.

 One Point Linear

ASTD
Formula: A =kC, K =
C STD

This calibration rule requires only one standard to obtain the parameter k.

 Two Point Linear

A2 − A1 A − A1
Formula: A =kC+b, K = , b = A1 − ( 2 )C1
C 2 − C1 C 2 − C1

This calibration rule requires two standards. C1 and C2 are concentrations of standard 1

and 2; A1 and A2 are their absorbance.

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 Multipoint Linear

Formula: A =kC+b.

You can get values of k and b through the method of least squares.

This calibration rule requires at least 3 standards.

 Nonlinear

Draw a broken line passing through the standard concentration and its absorbance.

Measure the absorbance of the waiting samples and check relevant concentration on the

broken line.

This calibration rule requires at least 3 standards.

 Multipoint Percent

The maximum absorbance of the standards is 100%.

This calibration rule requires at least 3 standards as the multi-point broken line.

 Exponent

Formula: A = k ln C + b

This calibration rule requires at least 3 standards.

 Logarithm

bC
Formula: A = ke

This calibration rule requires at least 3 standards.

 Power function

b
Formula: A = kC

This calibration rule requires at least 3 standards.

f) Factor Setup: Enter the calibration parameters k and b which are mainly used in the factor

calculation method.

3.4.2. New Test

Touch the New Test button to select the test that you want to add or modify (refer to figure 4

for Select Test screen), and then enter the New Test screen (Figure 11).

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 Microscan + (Plate Reader) User Manual

New Test _____________

1ABCabc 2DEFdef

3GHIghi 4JKLjkl

5MNOmno 6PQRpqr

7STUstu 8VWXvwx

9Yzyz +-*/

0.,() NEXT

YES CLR

Exit

Figure 11 New Test screen

In the New Test screen, if you touch a button repeatedly, the screen will display the next

number, letter or symbol in circular way. NEXT is used to move the cursor

rightward to enter the next character. If you enter a wrong character, delete it with the

CLR button and enter again. Touch YES to save what you have entered and

Exit to return to the Program screen (Figure 7) without saving your entering.

3.4.3. Delete Test

Touch the Delete Test button, select the test that you want to delete (Figure 4).

3.5. Quality Control

Touch the Quality Control button, select the test that you want to view (Figure 4), then the QC

plot of this test will be displayed (Figure 12.). Touch the Settings button to set the QC parameters

of this test (V - Mean, SD - Standard Difference). If you want to print the QC data and QC plot, touch

the Print button.

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 Microscan + (Plate Reader) User Manual

hAFP Quality Control V：

： 0.000 SD：
：0.000
+3SD
+2SD

-2SD
-3SD

Settings Delete Data

Print Exit

Figure 12 QC Plot screen

After touching the Delete Data button, you need to select Del. Last Month or Del. All Data, as

shown in figure 13. Touch Exit to return to the screen as shown in figure 12.

Del. Last Month

Del. All Data

Exit

Figure 13 Delete Data screen

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3.6. Setup

In the Setup screen (Figure 14), you can set the date and time, vibrant speed, light source, printer and

filter.

Setup

Date and Time Vibrancy

Light Source Printer

Filter

Exit

Figure 14 Setup screen

3.6.1. Date and Time

Touch the Date and Time button to set Year, Month, Day, Hour and Minute one by one. Touch YES

to confirm your settings and proceed to the next option until you finish all settings.

3.6.2. Vibrancy

The Vibrancy screen (Figure 15) includes Vibrant Speed and Vibrant Time. There are three speeds

available as High, Normal and Low. The vibrant time should not be longer than 60s.

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 Microscan + (Plate Reader) User Manual

Vibrant Speed Normal

Vibrant Time 30s

Exit

Figure 15 Vibrancy screen

3.6.3. Light Source

This button is used to turn on/off the lamp temporarily for the sake of the lamp’s lifespan.

NOTE: The lamp should be turned on and preheats for 10 min before testing; otherwise the

alarm message “Lamp L” will appear.

3.6.4. Printer

This button is used to enable / disable the printer, so you can select whether to print the results immediately

after testing.

3.6.5. Filter

The Microplate Reader reads the air blank absorbance of each filter in the 4 channels (F5 is spare), which

is used to check the photometric system. The default absorbance of the 405 - 630 filters is less than 0.500.

However, it may increase gradually in the result of dust, humidification and moldiness when the device is

used for a long time. If the absorbance is greater than 1.000, the operator should clean the photometric

system or replace the accessories like the filters.

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3.7. Service

There are three options in the Service menu:

Data Transfer

Result Review

Standard Review

3.7.1. Data Transfer

Touch the Data Transfer button to transport the test data to the computer which is connected to the

Microplate Reader through the serial USB 2.0, RS232 port.

3.7.2. Result Review

Touch the Result Review button and enter the No. of Plate that you want to search, then touch

YES to display the results. Touch the button to display circularly the results of column 0-6

and 7-12.

3.7.3. Standard Review

After touching the Standard Review button and selecting the test that you want to search, the screen

will display the standard absorbance and relevant concentration, as well as the calibration curve, as shown

in figure 16. However, if the selected test doesn’t use the Quant Calculation Mode, the results will not be

displayed).

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 Microscan + (Plate Reader) User Manual

Standard Review hAFP

Concentration Absorbance ABS

1 1.0 1.000

2 2.0 2.000

3 3.0 3.000

4 4.0 4.000

5 5.0 5.000

6 6.0 6.000

7 7.0 7.000

8 8.0 8.000

k 1.000

b 0.000

Multipoint Linear STD

Exit

Figure 16 Standard Review screen

3.8. Rapid Measure

This function is used to read the absorbance of the waiting samples quickly. After touching the

Rapid Measure button, select the primary and secondary wavelength and enter the No. of samples.

Touch OK to start testing (Figure 17).

Prim. Wav. 450nm

Sec. Wav.

Vibrancy

Exit OK

Figure 17 Rapid Measure screen

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 Microscan + (Plate Reader) User Manual

4. Maintenance

4.1. Notice

To ensure reliable performance and excellent operation of the Microplate Reader, the operator must

operate and maintain the device strictly as instructed by this Operation Manual.

4.2. Maintenance

The Microplate Reader is a precision optical measuring system. Therefore, it’s necessary to perform

the daily maintenance, which includes:

1) Keeping the operating environment dry and clean. Covering the sample compartment after using

to prevent from dust.

2) Keeping the mechanical and electrical parts of the device away from water and reagents.

3) Switching off the power supply while cleaning the surface and screen of the device. Be sure to use

soft cloth with noncorrosive detergent.

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5. Technical Specifications

Wavelength: Accuracy (±2nm), Half width (≤12nm), Peak transmittance (≥35%).

Wavelength range: 405 nm, 450 nm, 492 nm, 630nm. 4 free positions for optional filters

Reading range: 0 - 3.5A, Measurement range: 0 - 2.5A

Absorbance resolution: 0.001A (Internally: 0.0001A)

Absorbance accuracy: ±0.03A (0 - 2.5A)

Repeatability (CV, %) ≤1%

Linearity: Error requirements

Wavelength Range (nm) Absorbance (A) Linearity Error %

400 - 750 0.0 - 2.5 ≤ ±2.5%

Stability: ≤ 0.005A/10min

Reading Speed: Single wavelength <20s/plate (96-well plate);

Dual wavelength <35s/plate (96-well plate).

Vibrancy: Vibrant Speed (High, Normal, Low);

Vibrant Time (0 - 60s).

Power supply: AC240V/100V, 50/60Hz , 2A

Power: 100VA

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6. Troubleshooting

Symptoms Causes Actions to Take

The power cord is not connected Re-connect the power cord

Can’t start up correctly properly

The fuse is broken Replace the fuse

The LCD display or inverter Contact our local distributor or

No display, no backlight
defective local agency

Remove the dust and replace the

The optical coupler is with dust
optical coupler
The sample compartment works or defective
Reposition the MICROPLATE
abnormally The sample compartment is
plate
blocked

Sound is abnormal during Foreign matter exists

Remove the foreign matter
operation Mechanical movement is
Contact our local distributor or
abnormal
local agency

The printer is not connected Check the connection between

Can’t print
properly the printer and the device

The lamp is aging or defective Replace the lamp

The filter is aging or defective Replace the filter

Readings are abnormal
The electric circuit defective Contact our local distributor or

local agency

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7. Warning

The Microplate Reader is a compact in vitro diagnosis instrument. It may bring risks to the patients

indirectly, and accompanying risks to the operator and environment. To avoid these risks, be sure

to read the following instructions carefully.

This device adopts AC 220V/110V which may bring electrical harm to human body. Don’t

open the rear cover before switching off the power supply. If you need to open the rear cover to

maintain the device, you’d better contact our service personnel or professionals.

For the patient samples may contain germina or virus which will cause disease, be

sure to dispose of the waste in accordance with relevant regulations of your hospital.

NOTE: Don’t operate the device unless you have read this Operation Manual carefully.

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CONTACT INFORMATION

Important: When contacting us, please have the Model and Serial Number of the instrument in question.
Have a description of the problem with as much detail as possible. Save any relevant printouts and send
or e-mail us the information.

Dealer:

Fortress Diagnostics Limited

Unit 2C Antrim Technology Park,

Belfast Road,

Antrim,

Northern Ireland,

BT41 1QS

United Kingdom

Tel: +44 (0)28 9448 7676

Fax: +44 (0)28 9446 9933

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