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Centrifuge 5418 R
Service manual - English
Copyright© 2015 Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part of
this publication may be reproduced without the prior permission of the copyright owner.

Centriplus® is a registered trademark of Millipore Corporation, Billerica, USA.

Microtainer® is a registered trademarks of Becton Dickinson, Franklin Lakes, NJ, USA.

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

5401 920.015-03/042015
 Table of contents
Centrifuge 5418 R 3
English (EN)

Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Hardware description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.2 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.3 Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.4 Lid latch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.5 Universal PCB control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.6 PCB converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.7 Motor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.8 Brake resistor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.9 Fan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.10 Refrigeration unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2 Machine marking and serial number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.2 Notes on liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.3 Safety notes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.4 Sicherheitshinweise am Gerät . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.1 Overview of functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.2 Activation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.3 SE 1 (Measurement of acceleration time and braking time). . . . . . . . . . . . . . . . . . . . . 22
5.2.4 SE 2 (Initializing of EEPROM). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.2.5 SE 3 (Test of lid latch) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2.6 SE 4 (Display test and key test). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2.7 SE 5 (Cyclical endurance test) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2.8 SE 6 (Displaying the error table). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2.9 SE 7 (Displaying the last 4 errors) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2.10 SE 8 (Displaying the operating hours and number of runs) . . . . . . . . . . . . . . . . . . . . . 25
5.2.11 SE 9 (Testing the overspeed cut-off) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.2.12 SE 10 (Test cooling aggregate, temperature sensor, fan) . . . . . . . . . . . . . . . . . . . . . . . 26
5.2.13 SE 13 (100 V device version setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
 Table of contents
4 Centrifuge 5418 R
English (EN)

5.3 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.3.1 Activation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3.2 Compressor / condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3.3 FC parameter compressor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3.4 FC parameter drive (1) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.5 FC parameter drive (2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.6 Lid signals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.7 Last error message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.8 Execution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.3.9 Current error message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

6 Disassembly/assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.1 Disassembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.1.1 Centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.1.2 Upper part of housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
6.1.3 PCB Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.1.4 PCB Frequency converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.1.5 Compressor fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
6.1.6 Brake resistor and temperature switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6.1.7 Motor and motor suspension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1.8 Condenser fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.1.9 Lid latch motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.10 Lid latch of microswitch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.1.11 Temperature sensor in rotor chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.2 Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

7 Alignment/adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

8 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
8.1 Overview electronics 5418R up to serial number 04823 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
8.2 Overview electronics 5418R from serial number 04824. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

9 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
9.1 Cleaning, disinfection, decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
9.2 Shipping the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
9.3 Service and inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
9.3.1 Essential Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
9.3.2 Advanced Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
9.3.3 Qualification Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
9.3.4 Additional Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
9.4 Device tests for centrifuges. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
9.4.1 Speed check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
9.4.2 Temperature check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
9.4.3 Timer check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
9.4.4 Electrical safety check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
 Table of contents
Centrifuge 5418 R 5
English (EN)

10 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
10.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
10.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
10.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
10.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
10.5 Tolerances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
11.1 Images of spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
11.2 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.2.1 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.2.2 Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.2.3 Lid latch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
11.2.4 Electronics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
11.2.5 Drive up to serial number 04823 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
11.2.6 Drive from serial number 04824 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
11.2.7 Fan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
11.2.8 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
11.2.9 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
11.3 Suggested spare parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

12 Technical information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
 Table of contents
6 Centrifuge 5418 R
English (EN)
 Operating instructions
Centrifuge 5418 R 7
English (EN)

1 Operating instructions
1.1 Using this manual

 Make sure that the service manual and the operating manual are available in the latest versions. To do
so, compare the version numbers. The latest versions of the manuals can be downloaded from
www.eppendorf-support.com and www.eppendorf.com.
 Read the service manual before commencing work on the device.
 Read the chapters "Installation" and "Operation" of the operating manual.
 Observe the safety instructions in the operating manual.

1.2 Danger symbols and danger levels

The safety precautions in these instructions have the following danger symbols and danger levels.

1.2.1 Danger symbols

Biohazard Explosive substances

Electric shock Risk of crushing

Cuts Hazard point

Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning
1. Actions in the specified order
2.
 Actions without a specified order
• List
Text Display text or software text
Additional information
 Operating instructions
8 Centrifuge 5418 R
English (EN)

1.4 Abbreviations used

Vcc
Voltage of the common collector.

rcf
Relative centrifugal force – g-force in m/s2

rpm
Revolutions per minute
 Product description
Centrifuge 5418 R 9
English (EN)

2 Product description
2.1 Hardware description
2.1.1 Chassis

The chassis is the foundation and supporting element of the entire construction. The chassis supports the
drive motor. The chassis consists of a bottom plate and PCB console. The PCB console is permanently
riveted to the ground plate on the right side.

The PCB console contains:

• Insulated rotor chamber
• PCB converter
• Brake resistor
• Heat sink (only 100 V version)

2.1.2 Housing

The centrifuge housing consists of the upper part of housing, lid covering and base covering. All three parts
are made from Bayblend FR3010 and meet the requirements of UL fire protection class V0.

In the housing are ventilation slits. The ventilation slits are located in the front underneath the keypad and
at the top behind the lid. The air for cooling the condenser and electronic components is directed from
front to back through the ventilation slits.

The housing screw connections are equipped with covers.

2.1.3 Lid

The complete lid assembly consists of a metal lid and lid covering. The metal lid is made of edged,
surface-treated sheet metal.

The metal lid includes a foamed insulation layer. A monitoring glass is located in the middle of the lid; the
magnet for the lid latch reed switch is located on the front right edge of the lid.

Oblong recesses are located to the right and left of the front side of the metal lid. The locking hooks grip in
these recesses.

The lid relief contains four leg springs, which automatically lift up the lid and hold it open.
 Product description
10 Centrifuge 5418 R
English (EN)

2.1.4 Lid latch

All lid latch components are mounted to the lid latch console. The lid latch console is a piece of galvanized
sheet metal which is vertically mounted to front of the bottom plate.

The lid latch is made of two locking hooks. The hooks are connected via an axis and synchronously travel a
defined curved track. The drive is a lid latch motor. An additional gear segment is used to transmit power
between the motor and the locking hook. The housing Reed sensor and the magnet in the metal lid provide
the signal for the lid latch to close. The lid latch motor starts at a max. distance of 10 mm and the locking
hooks travel to the closed position. After this end position has been reached, the left locking hook activates
a microswitch. The microswitch indicates that the lid is securely closed. The centrifuge can only be started
after the control has received this signal. To detect a failure of the microswitch, the lid latch travels to the
"open" position every time the centrifuge is switched on. As signals from the sensor and magnet are
required to start the lid latch motor, the lid latch will not be activated if the Reed sensor or magnet are
defective.

The lid latch can also be unlocked using the emergency release when it is deenergized or switched off. The
emergency release is located on the right side behind a plastic cover. The emergency release must be used
operated using the rotor key.

2.1.5 Universal PCB control

The condenser fan, compressor fan, and processor for controlling the converter, are located on the PCB
control. The signals from the motor (speed) and temperature sensor in the condenser and rotor chamber
are recorded here and evaluated by the software. The PCB control is mounted onto the upper part of the
housing from the inside using four screws.

2.1.6 PCB converter

The PCB converter is located at the right rear, vertically mounted to the PCB console. The PCB converter
contains two processors. One processor controls the compressor using parameters from the PCB control.
The second processor is responsible for controlling the drive motor.

2.1.7 Motor

The drive motor is a 3-phase electric motor. The motor is mounted to 3 horizontally bolted rubber metal
mounts. The rubber metal mounts are screwed onto a plate, which is mounted onto the chassis bottom
plate from below.

To control the speed, a speed sensor is integrated into the motor.

The upper end of the motor shaft has a cone and thread to hold the rotor. The rotor is secured using this
screw connection and friction-locked onto the cone by applying pressure.
 Product description
Centrifuge 5418 R 11
English (EN)

2.1.8 Brake resistor

The brake resistor provides a controlled reduction in motor speed. Two different brake resistors are used
depending on the voltage version of the device: one for 230 V/120 V and one for 100 V. The brake resistor
is monitored by a sensor (thermoswitch). If the temperature increases above a specific value, the
thermoswitch opens and the centrifuge switches off completely, i.e. the current supply is interrupted.

2.1.9 Fan

The centrifuge contains two fans. The main fan is located behind the condenser and removes the air from
the front ventilation slits. First, the suctioned air flows through the condenser and then the drive motor. A
second fan blows the air out of the device via the upper rear ventilation slit.

2.1.10 Refrigeration unit

The refrigeration unit is composed of: lifting piston compressor, condenser (two-row), R134a refrigerant,
evaporator and dryer. A capillary tube is the throttle device for pressure reduction. The evaporator tube is
wrapped around the coated rotor chamber and covered in insulation. The refrigerating capacity is
monitored by two temperature sensors positioned in the rotor chamber and on the condenser.
 Product description
12 Centrifuge 5418 R
English (EN)

2.2 Machine marking and serial number

Abb. 2-1: Eppendorf AG machine marking using Centrifuge 5418 as an example

12 1

11 2

10 3

9 4

8 5

7 6

Fig. 2-1: Eppendorf AG machine marking using Centrifuge 5418 as an example

1 Manufacturer's address 7 CE mark

2 Consecutive number within the 12-digit serial 8 Power consumption
number
9 Permitted frequency
3 Maximum speed
10 Permitted voltage
4 Maximum kinetic energy
11 Product number
5 Maximum density of liquid
12 Manufacturer
6 Quality seal
 Safety
Centrifuge 5418 R 13
English (EN)

3 Safety
3.1 User profile

The specialist entrusted with maintenance, repair or testing must meet the following prerequisites:
• Successful participation in service trainings with certification by Eppendorf AG for the product that is to
be maintained, repaired or tested.
• Qualification as a specialist with knowledge of the applicable local and international standards.
• Qualification to evaluate the work assigned to him.
• Recognition of potential hazards and how to prevent or correct them.

3.2 Notes on liability

The Eppendorf service organization entrusted with maintenance, servicing or testing is liable for ensuring
that all work is carried out in a professional manner.

 Repair, maintenance and testing may not change the existing construction of the device. The device
must always be as safe as it is in its original state.
 Only accessories and original spare parts recommended by Eppendorf, and measuring and test
equipment recommended by Eppendorf may be used for any maintenance, servicing or testing work.

3.3 Safety notes

DANGER! Electric shock.

 Switch the device off.

 Unplug the power plug.
 Then begin repairing, servicing or cleaning the device.

DANGER! Risk of explosion.

 Do not operate the device in areas where work is completed with explosive substances.
 Do not use this device to process any explosive or highly reactive substances.
 Do not use this device for processing any substances which could generate an explosive
atmosphere.

WARNING! Infection by contaminated material.

There may be contaminated material on the device and accessories. Risk of infection with
contaminated material.

 Find out more about contamination risks before beginning work.

 Check the device decontamination certificate.
 Work may only be completed on a decontaminated device.
 Wear personal protective equipment (protective gloves, protective goggles).
 Safety
14 Centrifuge 5418 R
English (EN)

WARNING! Risk of injury when opening or closing the centrifuge lid.

There is a risk of crushing your fingers when opening or closing the centrifuge lid.

 When opening or closing the centrifuge lid, do not reach between the lid and device or into
the latching mechanism of the lid.
 Always open the centrifuge lid completely to prevent it from falling.

CAUTION! Injury from sharp-edged components.

The opened device contains sharp-edged components. Risk of injury from sharp-edged
components.

 Wear protective gloves.

 Work with extreme care.

NOTICE! Device damage due to unprofessional repairing of the refrigerating system.

 The refrigerating system must be repaired by a refrigeration specialist.

 Repairs, e.g. spare parts replacement, are carried out on one's own responsibility.
 Observe national and local safety regulations.

NOTICE! Damage to the compressor and refrigerating system due to tilting the device.
The compressor and refrigerating system can become damaged if the device is switched on
when it is tilted or if the device is switched on right after it was tilted.

 Leave the device in the normal position for at least 4 hours before switching it on.
 Never place the device upside down.

3.4 Sicherheitshinweise am Gerät

Darstellung Bedeutung Ort

Gefahrenstelle 5418 R: Rechte Geräteseite

 Bedienungsanleitung beachten.
VORSICHT Geräteoberseite, unter dem
Zentrifugendeckel.
 Den Rotor immer mit dem beigelegten
Rotorschlüssel festziehen.
Warnung vor biologischen Risiken beim Umgang Aerosoldichte Rotoren/
mit infektiösen Flüssigkeiten oder pathogenen Rotordeckel, aerosoldichte
Keimen. Becher/Kappen.
 Operation
Centrifuge 5418 R 15
English (EN)

4 Operation
4.1 Overview of operating controls

Before using the Centrifuge 5418 / 5418 R for the first time, familiarize yourself with the operating controls
and the display.

Abb. 4-1: Control panel of the Centrifuge 5418 and Centrifuge 5418 R.

1 2 3 4 (9) 2 5 (1)

5418 5418 R
4 5 9 8 8 6 7 6 3

Fig. 4-1: Control panel of the Centrifuge 5418 and Centrifuge 5418 R.

1 Switch the displayed centrifuging speed (rpm/ 5 Set speed of centrifugation

rcf) Only 5418 R: Press the speed dial to switch the
displayed centrifugation speed (rpm/rcf).
2 Display
6 Adjust temperature (only 5418 R)
3 Release lid
7 Start the temperature control run fast temp
4 Set centrifugation time
(only 5418 R)
Only 5418 R: Press the time dial to start and stop
centrifugation. 8 Short-spin centrifugation
9 Start and stop centrifugation
 Operation
16 Centrifuge 5418 R
English (EN)

Abb. 4-2: Display of the Centrifuge 5418 and Centrifuge 5418 R.

1 2 3 1 4 5 6 2 3

5418 7 5418 R 8 7

Fig. 4-2: Display of the Centrifuge 5418 and Centrifuge 5418 R.

1 Centrifugation time 5 Temperature (only 5418 R)

2 G-force (rcf) or speed (rpm) 6 Status of the key lock (only 5418 R)
5418 R: Set value x 1000 : Centrifuging parameters cannot be modified
unintentionally.
3 Status of centrifuge
: No key lock.
: Centrifuge lid unlocked.
: Centrifuge lid locked. 7 Status of centrifugation speed display
(Flashes): centrifugation in progress. rcf: g-force (relative centrifugal force, RCF)
rpm: Speed (revolutions per minute)
4 At set rpm (only 5418 R)
: Start of run time when reaching 95% of the 8 Status of the loudspeaker (only 5418 R)
preset g-force (rcf) or speed (rpm). : Switched on.
: Immediate start of run time. No symbol: Switched off.
 Troubleshooting
Centrifuge 5418 R 17
English (EN)

5 Troubleshooting
5.1 Error messages

Problem Cause Solution

No function • Mains fuses defective.  Check/replace fuses.
• Power switch (main power inlet  Check/replace power input module.
module) defective.  Check the power supply.
• Power supply in the lab faulty.  Check/replace the temperature
• Temperature controller from the controller from the brake resistor.
brake resistor has interrupted the
power supply.
Er 3 • No rotational speed impulses at  Leave the centrifuge switched on and
start-up or during run. Speed wait until the countdown is
measuring system or drive faulty. completed.
• Motor overheated, temperature fuse  Let the motor cool down.
has interrupted the phases.  Check speed sensor (customer service
function 4).
Note: If this error occurs during the run,
 Check the motor and replace it if
the centrifuge will decelerate in an
necessary.
unbraked manner and will remain locked
 Check the PCB converter and replace
for 7.5 minutes. The error message and
it if necessary.
countdown are displayed one after the
other. The lid can only be operated or
opened after the countdown has been
completed.
Er 5 • No feedback from the lid latch after  Check lid latch (service function SE
closing the lid or during the run. 3).
 Check/adjust/replace reed contact.
Note: Two switches in the lid latch will be
 Check/adjust/replace microswitch.
queried (microswitch and Reed contact).
 Check/replace lid latch motor.
Er 6 • Frequency converter fault or thermal  Allow the centrifuge to cool down,
overload. and restart it.
• No ready signal from the frequency  Check the connection between the
converter. PCB control and PCB converter.
• The frequency converter has  Check/replace PCB converter.
performed a reset (power failure).  Check/replace brake resistor.
• The supply voltage is too low.  Check the supply voltage.
 Troubleshooting
18 Centrifuge 5418 R
English (EN)

Problem Cause Solution

Er 7 • The overspeed control has been  Let the motor cool down.
triggered. The maximum speed was  Check/replace PCB converter.
exceeded.  Check/replace motor.
• Speed deviation > ± 800 rpm from the
specified value.
• Motor overheats during the run.
Er 8 • Ramp error (time error) during  Let the motor cool down.
acceleration.  Check/replace motor.
• The set speed is not reached.  Replace PCB converter.
• Motor overheated during the
acceleration.
Er 9 • Write error on EEPROM.  Initialize the device (service function
SE 2).
 Replace PCB control.
Er 10 • Read error on EEPROM.  Initialize the device (service function
SE 2).
 Replace PCB control.
Er 11 • Watchdog reset or brief power failure  Check network connections.
during a run.  Check ground connections.
 Initialize the device (service function
SE 2).
 Check the connection between the
PCB converter and PCB control.
 If this error occurs again, replace PCB
control or PCB converter.
Er 14 • EEPROM check sum error after power  Initialize the device (service function
supply has been activated. SE 2).
• Error in the program memory,  Check/replace PCB control/controller.
EEPROM faulty.
Er 16 • Communication error between the  Check the connection between the
Er 17 PCB control and PCB converter. PCB control and PCB converter.
 Initialize the device (service function
SE 2).
 Check/replace PCB converter.
 Check/replace PCB control.
Er 18 • Excess temperature in the rotor  Check/replace compressor (service
chamber (chamber temperature >50 function SE 10).
°C).  Check/replace temperature sensor
• After the temperature has been (service function SE 10).
adjusted, the ±5 °C interval is exited  Check/replace fan (service function
again. SE 10).
• Temperature sensor in the rotor
chamber measures incorrectly.
 Troubleshooting
Centrifuge 5418 R 19
English (EN)

Problem Cause Solution

Er 19 • Excess temperature in the condenser  Allow the cooling unit to cool down.
(condenser temperature >58 °C).  Check the plastic rotor chamber.
• Condenser contaminated. Clean condenser.
• Air supply to the housing  Clean/open air supply in the housing.
contaminated or closed.  Ensure there is a sufficient amount of
• Distance to other devices or walls, space between the condenser and
etc., is too small. other devices or walls, etc.
• Condenser fan defective.  Check/replace condenser fan (service
• Temperature sensor on the condenser function SE 10).
measures incorrectly.  Check/replace temperature sensor
(service function SE 10).
 Check whether the condenser fan
starts when the cooling unit is turned
on.
Er 20 • Temperature sensor (NTC) in the  Check/replace temperature sensor
chamber defective. (service function SE 10).
Er 21 • Temperature sensor (NTC) on the  Check/replace temperature sensor
condenser defective (measured value (service function SE 10).
is outside of –8 °C .. +83 °C).
Er 22 • Error in the fan control.  Check/replace fan (service function
SE 10).
 Check/replace PCB converter.
Er 23 • No recognition on the EEPROM.  Conduct PCB initialization.
• Device has not been initialized.  Replace PCB control/controller.
Note: Error message only in service
mode! Error will not be saved.
Er 24 • Error in the compressor control  Check plug connection to the
(compressor cannot be started). compressor.
 Check the refrigerating system/
compressor using service function SE
10.
 Check/replace PCB converter.
 Replace the compressor. The
compressor may only be replaced by
a refrigeration technology specialist!
Er 25 • Power failure during a run.  Check/replace power input module.
 Check/replace power switch.
 Check/replace PCB converter.
 Troubleshooting
20 Centrifuge 5418 R
English (EN)

Problem Cause Solution

Er 30 • Lid could not be latched or unlatched.  See Er 5.
(only in service • At least one switch response is
mode) missing when the lid is closed and the
centrifuge is at a standstill.
Note: Error will not be saved.
NO RPM • Error in speed measuring system.  See Er 3.
LID • Error in the lid latch.  See Er 5.
Note: Er 30 will be displayed in the
service mode! Error will not be saved.
The wear period and current
consumption of the lid latch motor will be
monitored. The device will open if a
time-out occurs or if the current
consumption is too high.
INT • Power failure during a run.  See Er 25.
Note: Er 25 is displayed in the service
mode.
Off • Displayed after power off. The
operating mode of the centrifuge
(acceleration, deceleration, standstill)
determines how long the control
remains active.
 Troubleshooting
Centrifuge 5418 R 21
English (EN)

5.2 Service functions

5.2.1 Overview of functions

Program number Program function

SE 1 Measurement of acceleration and braking time
SE 2 Initialization of EEPROM
SE 3 Test of lid latch
SE 4 Test of display and keys
SE 5 Cyclical endurance test
SE 6 Display of error table
SE 7 Display of the last 4 errors
SE 8 Display of operating hours and quantity of centrifuge runs
SE 9 Test of overspeed cut-off
SE 10 Test of cooling unit, temperature sensors and fan
SE 11 No. service function
SE 12 No. service function
SE 13 100 V device version setting

5.2.2 Activation and operation

1. Press the rpm/rcf key and simultaneously switch on the device. Keep the rpm/rcf key pressed until SE 1
is displayed.
2. Turn the time rotary knob for selection of the individual service functions.
3. Switch off the centrifuge to exit the service functions.
 Troubleshooting
22 Centrifuge 5418 R
English (EN)

5.2.3 SE 1 (Measurement of acceleration time and braking time)

 Activate the service functions, using the start/stop key.

A short display check is performed. All emblems light up for 2 s. Then the display changes
automatically.

Display 1:

1 2 - - x x. x

• xxx: Maximum rotational speed of centrifuge x 1000

 Start the measurement run, using the start/stop key.

The device accelerates to max. speed. The acceleration time is being displayed:

Display 2:

H L y y y

• yyy: Acceleration time in seconds

 Stop the measurement run, using the start/stop key

The device brakes to a standstill. The braking time is being displayed:

Display 3:

b r z z z

• zzz: BT (braking time) in seconds

 After the centrifuge has come to a standstill, press the short key to exit the service function.

5.2.4 SE 2 (Initializing of EEPROM)

 Activate the service functions, using the start/stop key.

Display:

I n I t

 To cancel without initialization: Press the start/stop key.

 To perform the initialization: Press the rpm/rcf key.
The standard display values are set. The error table, operating hours, number of runs and table of the
last errors are deleted.
The service function is exited automatically after the initialization has been successfully completed.
 Troubleshooting
Centrifuge 5418 R 23
English (EN)

5.2.5 SE 3 (Test of lid latch)

 Activate the service functions, using the start/stop key.

Display:

L I D x y

• y: Microswitch (0 = lid released, 1 = lid locked)

• y: Reed contact (0 = lid open, 1 = lid locked)

Press the following keys to perform the individual functions:

 rpm/rcf key: The lid latch motor is driven step-by-step.

After 5 to 10 steps, the lid is completely locked and the display (c) switches from 0 to 1.
 rpm/rcf key: Releases the lid latch in one step (requirement: x = 1 and y = 1).
 open key: Releases the lid latch from any position.

 Press the short key to exit the service function.

5.2.6 SE 4 (Display test and key test)

 Activate the service functions, using the start/stop key.

All segments and symbols are displayed simultaneously. The display changes after a few seconds.

Display 1:

8 8 8 8 1 8 8

Display 2:

I n

 Press all keys in succession.

Each key controls a digit of the display. After pressing the start/stop key, the service function is exited
automatically after several seconds.
 Troubleshooting
24 Centrifuge 5418 R
English (EN)

5.2.7 SE 5 (Cyclical endurance test)

 Activate the service functions, using the start/stop key.

Display:

x x y y z z z

• xx: Time
• yy: Temperature
• zz: Rotational speed

4 runs are performed with a 1-min. break between each run. Followed by a longer break.

After the first run has been started, the other starts are carried out automatically.

The parameters can be changed The cyclical endurance test should be performed with using the preset
values.

Press the following keys to perform the individual functions:

 fast temp key: Change the duration of the longer break in increments of 1 to 20 min (only possible
before the start of the 1st run).
 start/stop key: Start of the cyclical endurance test
 start/stop key: Stop of a run.

 During a break, press the short key to exit the service function.

5.2.8 SE 6 (Displaying the error table)

 Activate the service functions, using the start/stop key.

All error numbers and the frequency of each error are displayed. The display changes once per second.
After the last error number, the service function is automatically exited.
 Press the start/stop key to prematurely interrupt the run and exit the service function.

Display:

E r x x y y y

• xx: Error number

• yyy: Number of errors

The error table can be deleted by performing an initialization (SE 2).

 Troubleshooting
Centrifuge 5418 R 25
English (EN)

5.2.9 SE 7 (Displaying the last 4 errors)

 Activate the service functions, using the start/stop key.

The last four errors are displayed in the order of occurrence. The display changes every second.After
the 4th error, the service function is exited automatically.
 Press the start/stop key to prematurely interrupt the run and exit the service function.

Display:

x E y y z z

• x = 1: Last error
x = 2: Error before last
etc.
• yy: Error number
• zz: ELCB error code

The last four errors can be deleted by performing an initialization (SE 2).

5.2.10 SE 8 (Displaying the operating hours and number of runs)

 Activate the service functions, using the start/stop key.

The operating hours and number of runs are displayed consecutively. Both displays appear for approx.
2 s. Then, the service functions is automatically exited.

Display 1:

x min x x x x
sec

• xxxxx: Operating hours

Display 2:

y y y y y y

• yyyyy: Number of runs

The operating hours and the number of centrifuge runs can be deleted by performing an
initialization (SE 2).
 Troubleshooting
26 Centrifuge 5418 R
English (EN)

5.2.11 SE 9 (Testing the overspeed cut-off)

NOTICE! Damage to device caused by excessive speed.

The centrifuge exceeds the maximum speed. The motor does not switch off.
 Disconnect the centrifuge from the power supply.

The test must be carried out without rotor.

 Activate the service functions, using the start/stop key.

Display:

x x y y z z z

• xx: Time
• yy: Temperature
• xxx: Maximum rotational speed of centrifuge + 500 rpm

 Close the centrifuge lid and start the test run, using the start/stop key.
The device accelerates to cut-off speed and must switch off in conjunction with Error 7. Deceleration is
performed unbraked.
 After the centrifuge has come to a standstill, press the short key to exit the service function.

5.2.12 SE 10 (Test cooling aggregate, temperature sensor, fan)

 Activate the service functions, using the start/stop key.

Display 1:

b y L z z

• y: Compressor (0 = switched off, 1 = switched on)

• zz: Fan (-- = switched off, 0...32 = switched on with displayed program level)

Press the following keys to control the individual functions:

 fast temp key: Switches the compressor on and off.
 open key: Switches the fans on and off.
 Arrow keys temp: Switching on the fan levels (level 1...32).
 short key: Switching to the display of the temperature sensor (and back).
Display 2:

x x x y y. y

• xxx: Condenser temperature with one decimal place

• yyy: Chamber temperature

 Exit the service function using the start/stop key.

 Troubleshooting
Centrifuge 5418 R 27
English (EN)

5.2.13 SE 13 (100 V device version setting)

Prerequisites
The setting of the 100 V device version can only be performed in conjunction with a 100 V/120 V converter
and from firmware version 1.1 upwards.

 Activate the service functions, using the start/stop key.

Display 1:

x x x

• xxx = 0: recent setting = 120 V

• xxx = 100: recent setting = 100 V

Press the following keys to perform the individual functions:

 rpm/rcf key: Switching to 100 V.

 fast temp key: Resetting to 120 V.

 Exit the service function, using the short key.

 Troubleshooting
28 Centrifuge 5418 R
English (EN)

5.3 Customer service functions

5.3.1 Activation and operation

1. Press the rpm/rcf key for more than 6s to activate the service displays.
2. Briefly press the rpm/rcf key to switch through the functions in rotation.
3. Press the rpm/rcf key again for more than 6s to exit the service displays.

The service displays can be accessed in any operating state.

5.3.2 Compressor / condenser

2. y min y x x. x rpm
sec

• yy: Fan level (-- fan off)

• xx.x: Condenser temperature (cooling unit switches off at 58 °C)
• min: FC compressor ready
• sec: Compressor is running

5.3.3 FC parameter compressor

3. V min x x y y rpm
sec rcf

• xx: Program-internal parameter

• yy: Rotating field speed of the compressor
• v: Program-internal parameter (temperature)
• min: FC compressor is ready
• sec: Compressor is running
• rpm: Limitation of compressor frequency to max. 65 Hz for mains voltage of approx. 96 V to 110 V
• rcf: Limitation of compressor frequency to max. 60 Hz for mains voltage of < 96 V
 Troubleshooting
Centrifuge 5418 R 29
English (EN)

5.3.4 FC parameter drive (1)

4. y min x x x rpm
sec rcf

• xxx: With open lid: actual rotational speed -> turn rotor by hand
• y: Internal drive status
• min: FC drive is ready
• sec: Release issued for FC drive
• rpm: Pulsing switched on
• rcf: FC drive error message

5.3.5 FC parameter drive (2)

5. x min x y y y

• xx: Frequency converter temperature (both power supplies)

• yyy: Intermediate circuit voltage
• min: Temperature warning

5.3.6 Lid signals

6. u x x y z

• y: Lid latch switch (0=open, 1=closed)

• z: Lid switch (0=open, 1=closed)
• x: Machine status (internal)
• u: Program-internal variable (DV)

5.3.7 Last error message

7. x x °C y y

• xx: Last occurred error

• yy: Second to last error message, etc.
• °C: If a FC error code was detected with Error 6/16/17, it will be alternately displayed with the error
number
 Troubleshooting
30 Centrifuge 5418 R
English (EN)

5.3.8 Execution

8. x x x x

• xxxx: Checksum EPROM 0...FFFDH

5.3.9 Current error message

This display can only be accessed in case of errors.

9. E x x y y rpm
rcf

• xx: Error number

• y: Number for more precise localization of the cause
• yy: FC error number, if the converter with an error switches off with Error 6
• rpm: FC error number drive
• rcf: FC error number compressor
– 1 - Low voltage
– 2 - Overvoltage
– 4 - Excess temperature converter
– 7 - Converter overcurrent/intermediate circuit overload
– 10 - No release, lid switch open (only drive)
– 40 - Short-circuit disconnection
– FA - Reset via Watch Dog
– FF - Serial interface Time Out
 Disassembly/assembly
Centrifuge 5418 R 31
English (EN)

6 Disassembly/assembly
6.1 Disassembly

DANGER! Electric shock.

 Switch the device off.

 Unplug the power plug.
 Then begin repairing, servicing or cleaning the device.

WARNING! Infection by contaminated material.

There may be contaminated material on the device and accessories. Risk of infection with
contaminated material.

 Find out more about contamination risks before beginning work.

 Check the device decontamination certificate.
 Work may only be completed on a decontaminated device.
 Wear personal protective equipment (protective gloves, protective goggles).

CAUTION! Injury from sharp-edged components.

The opened device contains sharp-edged components. Risk of injury from sharp-edged
components.

 Wear protective gloves.

 Work with extreme care.

NOTICE! Device damage due to unprofessional repairing of the refrigerating system.

 The refrigerating system must be repaired by a refrigeration specialist.

 Repairs, e.g. spare parts replacement, are carried out on one's own responsibility.
 Observe national and local safety regulations.

NOTICE! Damage to the compressor and refrigerating system due to tilting the device.
The compressor and refrigerating system can become damaged if the device is switched on
when it is tilted or if the device is switched on right after it was tilted.

 Leave the device in the normal position for at least 4 hours before switching it on.
 Never place the device upside down.

Tools
• Phillips screwdriver size 1, 2
• Flat head screwdriver ca. 0.5x3
• Open-end wrench SW 4, 7, 8, 10
• Allen screwdriver SW 5 (rotor key)

Perform the owing disassembly steps in the sequence described for each component:
 Disassembly/assembly
32 Centrifuge 5418 R
English (EN)

6.1.1 Centrifuge lid

Abb. 6-1: Centrifuge lid

4
5

Fig. 6-1: Centrifuge lid

1 Lid cover 4 Lid springs (4 pcs.)

2 Metal lid 5 Lid axis
3 Assembly screws (4 pcs.) 6 Reed magnet

1. Open the centrifuge.

2. Unscrew the assembly screws of the lid covering and remove the lid covering. Video
3. Remove the retaining clip from the lid axle on one side and the remove lid axle. Video
4. Remove the metal lid and the lid spring upwards. Memorize the position of each individual lid
spring!(Video Step 3)
Make sure the correct assembly position is used during the assembly.The long end of the lid
springs is located in the upper part of housing; the short end is in the centrifuge lid.
 Disassembly/assembly
Centrifuge 5418 R 33
English (EN)

6.1.2 Upper part of housing

Prerequisites
The centrifuge lid has been removed.

Abb. 6-2: Upper part of housing / housing screws

Fig. 6-2: Upper part of housing / housing screws

1 Upper part of housing 3 Sealing for chamber

2 Housing screws (4 pcs.); covered underneath
the cover caps

1. Remove the cover caps of the housing screws and unscrew the housing screws. Video
2. Slightly lift the upper part of housing and immediately position it on the right side of lower part.
Video
3. Disconnect the connection to the upper part of housing: Video
• Ground connection PCB Control to the lower part of housing.
• Ribbon cable on the PCB converter.
• Lid latch motor and lid switch port.
• Connection cable sensor (rotational speed and temperature).
 Disassembly/assembly
34 Centrifuge 5418 R
English (EN)

Make sure the cables in the front right area are not damaged by the lid latch during the
positioning of the upper part of housing!Position the cable inside the cable holders! (see
Fig. 6-3 on p. 34)
Make sure the sealing for chamber is correctly put over the chamber rim during the assembly.
Make sure the upper part of housing is positioned in the provided groove inside the sealing for
chamber.
Abb. 6-3: Cable of the PCB Control

Fig. 6-3: Cable of the PCB Control

6.1.3 PCB Control

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

Abb. 6-4: PCB Control

Fig. 6-4: PCB Control

1 Port Reed contact 3 Ribbon cable to PCB frequency converter

2 Port temperature sensor, lid latch motor and
microphone switch
 Disassembly/assembly
Centrifuge 5418 R 35
English (EN)

Abb. 6-5: Back of PCB Control

Fig. 6-5: Back of PCB Control

1 PCB Control 3 Reed contact

2 Assembly screws (4 pcs.)

1. Pull off both rotary knobs. Video

2. Unscrew the PCB Control assembly screws and remove the connection cable from the clips.
Video
3. Lift the PCB carefully to prevent the display, key and pipetting lever from being damaged. (Video Step 2)
The pipetting levers of the keys are colored differently and also have different dimensions.
Interchanging the pipetting levers may cause malfunctions!
 Disassembly/assembly
36 Centrifuge 5418 R
English (EN)

6.1.4 PCB Frequency converter

Prerequisites
The centrifuge lid and the upper part of housing have been removed.
Abb. 6-6: PCB Frequency converter

10 12 11

1 10

4 5
9

5 6 7 8

Fig. 6-6: PCB Frequency converter

1 Port ribbon cable to PCB Control 8 Connection to brake resistor

2 Connection to speed sensor 9 Rear side of assembly screws M4x8
3 Connection cable of motor 10 Assembly screws M4x12 (with spacer sleeves
between the PCB frequency converter and
4 Assembly screws M3x25
chassis)
5 Assembly screws M4x8
11 Connection to port condenser fan
6 Port network entry
12 Port compressor fan
7 Connection to cable compressor

1. Detach all connection cables to the PCB frequency converter. Video

2. Unscrew the assembly screws and remove the PCB frequency converter. Video
Both M4 screws (position 4 (see Fig. 6-6 on p. 36)) must be removed before assembling the
new PCB frequency converter.The M4 screws must be replaced by the two M3 screws that had
fixed the original PCB frequency converter.
 Disassembly/assembly
Centrifuge 5418 R 37
English (EN)

6.1.5 Compressor fan

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

Abb. 6-7: Compressor fan

Fig. 6-7: Compressor fan

1 Compressor fan 2 Assembly screws (3 pcs.)

1. Detach the connection cable. Video

2. Unscrew the assembly screws and remove the compressor fan. Video

Mind the assembly position during the assembly. The air flow must be directed upwards.
 Disassembly/assembly
38 Centrifuge 5418 R
English (EN)

6.1.6 Brake resistor and temperature switch

Prerequisites
Centrifuge lid, upper part of housing and compressor fan have been removed.

Devices from serial number 04824 upwards will provide a different brake resistor. The
temperature switch will be dropped from this serial number upwards.
The new brake resistor is independent from the network voltage (see p. 71).
Abb. 6-8: Brake resistor with temperature switch

2
1

Fig. 6-8: Brake resistor with temperature switch

1 Brake resistor 2 Temperature switch

1. Detach the connection cable. Video

2. Unscrew the assembly screws and remove the brake resistor and the temperature switch. Video
 Disassembly/assembly
Centrifuge 5418 R 39
English (EN)

6.1.7 Motor and motor suspension

Prerequisites
The centrifuge lid and the upper part of housing have been removed.
Abb. 6-9: Motor and motor suspension

6
3

2 4 5

Fig. 6-9: Motor and motor suspension

1 Motor base plate 4 Rubber-bonded metal buffer (motor

suspension)
2 Assembly screws (4 pcs.)
5 Nut
3 Motor
6 Motor plate connector

1. Detach the connection cable and the ground strap. Video

2. Place the centrifuge on the left side. (Video Step 1)
3. Unscrew the motor base plate and remove the motor with the motor base plate. Video
4. Detach the nuts on the rubber-bonded metal buffers (motor suspension) and remove the motor
upwards. Video
During the assembly, make sure the motor insulation is completely positioned inside the
groove of the motor plate connector.
The sandpaper discs act as an anti-twist protection. During the assembly, make sure the nuts
on the side of the sandpaper disc are tightened last. Otherwise, the rubber will become
warped and an imbalance can no longer be optimally detected.
 Disassembly/assembly
40 Centrifuge 5418 R
English (EN)

6.1.8 Condenser fan

Prerequisites
The centrifuge lid, upper part of the housing and motor have been removed.

Abb. 6-10: Condenser fan

Fig. 6-10: Condenser fan

1 Condenser fan 2 Assembly screws (2 pcs.)

1. Unscrew the assembly screws. Video

2. Remove the condensator fan through the finger hole inside the base plate and detach the connection
cable. Video
Correct orientation must be observed during the assembly.The air flow must be directed into
the device or the required refrigerating capacity will not be achieved.
 Disassembly/assembly
Centrifuge 5418 R 41
English (EN)

6.1.9 Lid latch motor

Prerequisites
The centrifuge lid, upper part of the housing, motor and condenser fan have been removed.

Abb. 6-11: Lid latch

3 4

1 6

Fig. 6-11: Lid latch

1 Lid latch motor 4 Upper gear wheel segment

2 Latch hook 5 Emergency release of gear segment
3 Nut 6 Assembly screws (3 pcs.)

1. Unscrew the nut and remove spacer plate, latch hook and upper gear wheel segment. Video
2. Unscrew the assembly screws and remove the gear wheel segment of the emergency release.
Video
3. Unscrew the assembly screws from the lid latch motor and remove the lid latch motor. Also detach the
connection cable. Video
Memorize the assembly position of the upper gear wheel segment when disassembling!
Wrong assembly will cause a malfunction of the lid latch!
After assembly of the gear wheel segment, check whether both end stops (locked – unlocked)
are reached and detach the segment once more if necessary to correct the position of the
motor pinion.
 Disassembly/assembly
42 Centrifuge 5418 R
English (EN)

6.1.10 Lid latch of microswitch

Prerequisites
The centrifuge lid and the upper part housing have been removed.

Abb. 6-12: Lid latch microswitch

Fig. 6-12: Lid latch microswitch

1 Lid latch microswitch

1. Detach the connection cable.

2. Unscrew the assembly screws and remove the microswitch.

6.1.11 Temperature sensor in rotor chamber

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

1. Detach the connection cable (clip contacts in the plug).

2. Lift the temperature sensor out of the rubber bushing, performing an upward movement.

6.2 Assembly

For all assembly steps, the installation proceeds in reverse order of disassembly. During assembly, also
observe chapter 7 "Alignment/adjustment".
 Alignment/adjustment
Centrifuge 5418 R 43
English (EN)

7 Alignment/adjustment
 Alignment/adjustment
44 Centrifuge 5418 R
English (EN)
 Diagrams
Centrifuge 5418 R 45
English (EN)

8 Diagrams
8.1 Overview electronics 5418R up to serial number 04823
 Diagrams
46 Centrifuge 5418 R
English (EN)

8.2 Overview electronics 5418R from serial number 04824

 Maintenance
Centrifuge 5418 R 47
English (EN)

9 Maintenance
9.1 Cleaning, disinfection, decontamination

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect the power plug before starting cleaning or
disinfection work.
 Do not allow any liquids to penetrate the inside of the housing.
 Do not spray clean/spray disinfect the housing.
 Only plug the device back in if it is completely dry, both inside and outside.

WARNING! Infection by contaminated material.

There may be contaminated material on the device and accessories. Risk of infection with
contaminated material.

 Find out more about contamination risks before beginning work.

 Check the device decontamination certificate.
 Work may only be completed on a decontaminated device.
 Wear personal protective equipment (protective gloves, protective goggles).

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as strong and
weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.
 If the device has been contaminated by aggressive chemicals, immediately clean it by
means of a mild cleaning agent.

NOTICE! Corrosion from aggressive cleaning agents and disinfectants.

 Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.

 Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer
period of time.

For further information as well as a detailed description on how to perform cleaning,

disinfection and decontamination, please refer to the operating manual of the device.
Carefully read the respective sections of the operating manual before you start cleaning,
disinfection or decontamination.
 Maintenance
48 Centrifuge 5418 R
English (EN)

9.2 Shipping the device

WARNING! Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. You find it as a PDF file on our
website (www.eppendorf.com/decontamination).
2. Decontaminate all the parts you would like to dispatch.
3. Include the fully completed decontamination certificate in the packing.

NOTICE! Damage as a result of incorrect packing.

Eppendorf AG is not liable for damage caused by improper packing.

 The device may only be stored and transported in its original packing.

Transport packing can be ordered via your Eppendorf service organization. The order number
can be found in the chapter Ordering information.
 Maintenance
Centrifuge 5418 R 49
English (EN)

9.3 Service and inspection

Eppendorf offers maintenance services for centrifuges in the form of Performance Plans.

Performance Plans for centrifuges are available with the following levels:
• Essential Check
• Advanced Maintenance
• Qualification Service
• Additional Maintenance

For each Performance Plan level the maintenance services are clearly defined. This chapter shows which
maintenance services are defined and how much time needs to be scheduled in for them. All maintenance
services are described.

9.3.1 Essential Check

Number Verify Description

EC1 Checking the device for any damage.  Check the outside of the device for any damage.
EC2 Cleaning the device.  Clean the device on the outside as described in
the operating manual.
EC3 Refrigerated centrifuges  Empty the condensation water tray and clean it.
• Checking the condensation water (Not for Centrifuge 5920 R.)
tray.
EC4 Checking the lid latch.  Check that the lid latch the lid properly locked.
EC5 Checking the rotor detection. Check all of the available rotors.

 Insert the rotor.

 Check whether the centrifuge detects the rotor.
EC6 Performing a test run.  Insert rotor with accessories.
 Charge the rotor with the maximum number of
filled tubes.
 Only refrigerated centrifuges: Start run with key
fast temp.
The temperature of 6 °C should be achieved quickly
(depending on rotor, note lowest achievable
temperature).

Time required: 0.25 h – 0.5 h per device

 50

Group 1 Group 2 Group 3 Group 4

Non-refrigerated Refrigerated Non-refrigerated Refrigerated

9.3.2
No imbalance Imbalance Imbalance Imbalance
detection detection detection detection
Advanced Maintenance for Centrifuges
English (EN)
Maintenance

5418
5424
5452
5453
5430
5702
5804
5810

5415 R
5418 R
5424 R
5430 R
5920 R
Centrifuge 5418 R

5417 R 5427 R
5702 R 5702 RH
5804 R 5810 R
Nr. Service interval in month 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12
AM 01 Cleaning the device x x x x x x x x x x x x x x x x
AM 02 Cleaning the condensation water tray and the hose x x x x x x x x
AM 03 Checking the display x x x x x x x x x x x x x x x x
AM 04 Checking the keypad x x x x x x x x x x x x x x x x
Advanced Maintenance

AM 05 Checking the stability and the housing x x x x x x x x x x x x x x x x

AM 06 Checking the rotor bowl x x x x x x x x x x x x x x x x
AM 07 Cleaning and greasing the rotor bowl seal x x x x x x x x x x x x
AM 08 Checking latch mechanism, lid latch and emergency release x x x x x x x x x x x x x x x x
AM 09 Checking the lid springs, the gas springs and the lid shaft x x x x x x x x x x x x x x
AM 10 Cleaning and greasing the lid latch x x x x x x x x x x x x x x x x
AM 11 Checking the motor cap and the speed sensor x x x x x x x x
AM 12 Checking the motor sleeve x x x x x x x x
AM 13 Checking the motor and the motor shaft x x x x x x x x x x x x x x x x
AM 14 Checking the motor suspension x x x x x x x x x x x x x x x x
AM 15 Checking the imbalance detection x x x x x x x x x
AM 16 Checking motor fan, device fan and ventilation slots x x x x x x x x x x x x x x
AM 17 Checking the fuse holder x x x x x x x x x
AM 18 Checking the PCBs and the brake resistor x x x x x x x x x x x x x x x x
AM 19 Checking the condenser fan x x x x x x x x
AM 20 Checking the compressor and the heat exchanger x x x x x x x x
AM 21 Checking the temperature sensors x x x x x x x x
AM 22 Evaluating the device data and deleting, if required x x x x x x x x x x x x x x x x
AM 23 Software update x x x x x x x x x x
AM 24 Performing a test run x x x x x x x x x x x x x x x x
Time required 60 min 70 min 90 min 120 min
 Maintenance
Centrifuge 5418 R 51
English (EN)

Spare parts kits are available for servicing some devices. The spare parts kits should be used
up completely every 24 months.

AM 01 Cleaning the device.

 Clean the centrifuge. Cleaning is described in the operating manual and in the
supplement sheets.
AM 02 Cleaning the condensation water tray and the hose.
1. Empty the condensation water tray and clean it.
2. Check that the tube is unobstructed.
3. Carefully blow the tube out with compressed air, if required.
AM 03 Checking the display.

 Check the display using the corresponding service function.

Pay attention to the following:
• Defective pixels
• Background lighting
 Replace display, if required
AM 04 Checking the keypad.

 Check the keypad using the corresponding service function. Press all of the keys.
 Check the tactility. The pressure on all of the keys must feel the same.
 Replace keypad, if required.
AM 05 Checking the housing

 Clean the housing. Cleaning is described in the operating manual and in the supplement
sheets.
 Check the housing for cracks. Liquid may enter the centrifuge through cracks.
 Check the housing for any damage that endangers safety.
 Replace the housing, if required.
AM 05 Checking stability

 Check the device feet for stability.

 If cracks have developed on the device feet, replace them.
AM 06 Checking the rotor bowl.
Cleaning of the rotor bowl is described in the operating manual.
 Inspect the rotor bowl for damage.
 Check the plastic rotor bowl for cracks.
 Clean the metal rotor bowl. The rotor bowl is made from stainless steel or has a special
coating.
 Check the rotor bowl for glass breakage and clean, if required.
 If samples with a high concentration of sodium chloride (NaCl) are used, spots may
appear which look like rust deposits. Remove any rust deposits with a polishing paste.
Rust deposits occur if the cleaning instructions recommended in the operating manual
were not followed.
 Replace the damaged rotor bowl.
 Maintenance
52 Centrifuge 5418 R
English (EN)

AM 07 Cleaning and greasing the rotor bowl seal.

A description of the cleaning and care procedure can be found in the operating manual and
supplement sheets.
 Check the seal of the rotor bowl.
If the seal is defective, the lid will not close properly. More ambient air enters the rotor bowl.
The humidity entering the rotor bowl results in increased ice formation in the rotor bowl.
The cooling capacity of the centrifuge decreases.
Pay attention to the following:
• Seal with cracks
• Deformed seal
• Hardened seals
 Replace the damaged seal.
 Clean the seal of the rotor bowl.
 Maintain the seal of the rotor bowl, e. g. rub the seal with glycerin or grease.
AM 08 Checking the lid latch.

 Check the reed switch using the corresponding service function.

 Check the reed switch manually using a magnet.
AM 08 Checking the latch mechanism of the lid latch.

 Check the latch mechanism using the corresponding service function.

 Trigger the individual steps of the closing procedure by a separate keystroke.
Pay attention to the following parts:
• Lid latch
• Lid loops
• Slides and hooks
 Replace any worn parts.
AM 08 Checking the emergency release.

 Check the emergency release as described in the operating manual.

AM 09 Checking the lid springs and the lid shaft.

 Check the lid springs and the lid shaft for visible wear, e.g. chafe marks.
AM 09 Checking the gas spring.
The handling of the gas spring is described in the operating manual.

 Check the gas spring.

 Keep the centrifuge lid in the open position when the centrifuge is not in use.
 Always open the centrifuge lid completely. Do not allow shelving mounted above the
centrifuge to restrict its movement.
AM 10 Cleaning and greasing the lid latch.

 Clean the lid latch with a degreasing agent, e.g. alcohol. Do not use acidic substances.
 Maintenance
Centrifuge 5418 R 53
English (EN)

AM 11 Checking the motor cap.

 Replace the seals of the motor cap every two years. Only insert the motor cap of the
Centrifuge 5430 R with silicone rubber E41.
 Check the seals.
Pay attention to the following:
• Cracks
• Damage
• Centered, tension-free installation
Cracks and damage are caused by:
• Incorrectly sealed sealing clamp on the aerosol-tight caps.
• Tubes and jammed parts in the rotor bowl during centrifugation.
• Ingress of liquids.
AM 11 Checking the speed sensor.

 Insert the rotor.

 Check the rotor detection and the rotor relation using the corresponding service function.
Note: Check the speed according to the OQ.
AM 12 Checking the motor sleeve.
The ingress of liquid causes extensive damage.
 Check the motor sleeve
Pay attention to the following:
• Cracks
• Damage
• Correct installation to ensure tightness
AM 13 Checking the motor.

 Remove the rotor from the centrifuge.

 Turn the motor shaft by hand. Operating noises and stiff movement indicate wear on the
bearing.
 Remove the housing.
 Check the bearing shield of the motor for corrosion and cracks.
 Replace any damaged parts.
AM 13 Checking the motor shaft.

Formation of grooves and scratches on the motor shaft:

• The rotor is removed from the centrifuge without due care.
• The rotor is placed in the centrifuge without due care.
• The rotor screw is not tightened.
 Remove the rotor.
 Check whether the motor shaft is worn.
 Check whether the motor shaft is clean.
 Replace the motor, if necessary.
 Maintenance
54 Centrifuge 5418 R
English (EN)

AM 14 Checking the motor suspension.

If the shock mounts for motor are mounted horizontally, they are exposed to shearing forces
and the weight of the loaded rotor. The shock mounts may develop cracks or may become
deformed.
 Check the shock mounts for motor.
Pay attention to the following:
• Cracks
• Damage
• Deformation
 Replace the shock mounts for motor every two years.
 Only replace shock mounts for motor as a full set (3 pcs.).
AM 15 Checking the imbalance detection.
1. Adjust the imbalance switch as described in the Alignment/Adjustment chapter of the
service manual.
2. Using the corresponding customer service function, check whether the tolerance is
observed for the adjustment.
AM 16 Checking motor fan and device fan.

 Use a cleaning brush or compressed air to remove any dust and contamination. Remove
contamination with an industrial vacuum.
 Listen for running noises.
 Use the corresponding service function to select the different program steps for testing.
 If the centrifuge is used frequently, replace the fan as a precautionary measure.
AM 16 Checking the ventilation slots.

 Check all ventilation slots and openings. Clean them if required.

In addition, in Centrifuge 5804 and Centrifuge 5810:
 Insert the fixed-angle rotor.
 Switch on the device.
 Check whether the ventilation flap opens. Use the corresponding service function to do
this.
AM 17 Checking the fuse holder.

 Remove the fuses.

 Check the fuse holder contacts and the fuse mount for oxidation and deposits.
AM 18 Checking the PCBs and brake resistor.

 Use a cleaning brush or compressed air to remove any dust and contamination on the
PCB.
 Check the brake resistors using an Ohmmeter.
 Maintenance
Centrifuge 5418 R 55
English (EN)

AM 19 Checking the condenser fan.

 Use a cleaning brush or compressed air to remove any dust and contamination.
 Listen for running noises.
 Check the different control stages using the corresponding service function.
AM 20 Checking the compressor.

 Check switching the compressor on and off using the corresponding service function.
AM 20 Checking the heat exchanger.

 Use a cleaning brush or compressed air to remove any dust and contamination.
AM 21 Checking the temperature sensors.

 Display the current temperature on the display using the corresponding service function.
After the centrifuge has been at a standstill for an extended period with the lid open, the
displayed temperature must match the ambient temperature.
Note: Check the temperature according to the OQ.
AM 22 Evaluating the device data and deleting, if required.

 Call the following parameters with the corresponding service function or customer
service function:
• Error list
• Error history (last 4 errors)
• Number of activations
• Operating hours
• Total number of all rotor runs
• Number of runs per rotor type
Note: Which parameters are displayed depends on which centrifuge is checked.
AM 23 Software update
Software updates are provided free of charge as part of the maintenance to benefit from
improvements to the program.
A software update is performed by replacing an EPROM or via the USB interface.
You can download the latest software versions from the website
www.eppendorf-support.com.
AM 24 Performing a test run.
1. Insert the rotor with accessories.
2. Load the rotor with the maximum number of filled tubes.
3. Only refrigerated centrifuges: Start run by pressing the fast temp key. 6°C should be
reached quickly (depends on rotor, note the lowest temperature that can be achieved).
Additional information can be found in the operating manual.
 Maintenance
56 Centrifuge 5418 R
English (EN)

9.3.3 Qualification Services

Inspections Group 1 Group 2 Group 3 Group 4

Non-refrigerated Refrigerated Non-refrigerated Refrigerated
No imbalance No imbalance Imbalance Imbalance
detection detection detection detection
• 5418 • 5415 R • 5430 • 5417 R
• 5424 • 5418 R • 5702 • 5427 R
• 5452 • 5424 R • 5804 • 5430 R
• 5453 • 5810 • 5702 R
• 5702 RH
• 5804 R
• 5810 R
• 5920 R
Service interval OQ Every 12 months
Time required on 40 min 75 min 60 min 90 min
OQ
Time required on 30 min 60 min 45 min 75 min
IQ

The IQ checklist and the OQ checklist are available on the Internet at www.eppendorf-support.com
(password required) or on request.

You can find descriptions for the measurements in the chapter “Device tests for centrifuges".
 Group 1 Group 2 Group 3 Group 4
Non-refrigerated Refrigerated Non-refrigerated Refrigerated

9.3.4
No imbalance Imbalance Imbalance Imbalance
detection detection detection detection

Additional Maintenance for Centrifuges

5418
5452
5452
5453
5430
5702
5804
5810

5415 R
5418 R
5424 R
5430 R
5920 R

5417 R 5427 R
5702 R 5702 RH
Nr. Service interval in month 24 24 24 24 24 24 24 24 24 24 24 24 24 24 5810 R 5804 R
24 24
PS 01 Replacing the lid springs and the gas springs x x x x x x x x x x x x x x
Time required 15 min 15 min 15 min 15 min
PS 02 Replacing the seal of the rotor bowl x x x x x x x x x x x x
Time required 15 min 20 min 20 min
PS 03 Replacing the motor suspension x x x x x x x x x x x x x x x x
Additional Maintenance

PS 04 Performing an Imbalance compensation x x x x x x x x x

Time required 15 min 20 min 45 min 60 min
PS 05 Software update x x x x x x x x x x
Time required 15 min 15 min 20 min
PS 06 Electrical safety check x x x x x x x x x x x x x x x x
Time required 15 min 15 min 15 min 15 min
PS 07 Speed check x x x x x x x x x x x x x x x x
Time required 10 min 10 min 10 min 10 min
PS 08 Timer check x x x x x x x x x x x x x x x x
Time required 10 min 10 min 10 min 10 min
PS 09 Temperature check x x x x x x x x x x x x x x x x
Time required 50 min 70 min 50 min 70 min
PS 10 Compulsory testing in Germany (BGR 500) x x x x
Verification every year in operating state about 90 min about 120 min
Verification every 3 years in dissembled state about 150 min about 200 min
English (EN)
Maintenance
Centrifuge 5418 R
57
 Maintenance
58 Centrifuge 5418 R
English (EN)

Recommended service kits

• Spare parts kits are available for servicing some devices. The spare parts kits should be
used up completely every 24 months.

PS 01 Replacing the lid springs.

See chapter "Disassembly/assembly".
PS 01 Replacing the gas springs.
See chapter "Disassembly/assembly".
PS 02 Replacing the seal of the rotor bowl.
See chapter "Disassembly/assembly".
PS 03 Replacing the motor suspension.
See chapter "Disassembly/assembly".
PS 04 Performing an imbalance compensation.
See chapter "Alignment/adjustment" or Service function in chapter "Troubleshooting".
PS 05 Software update
A new software version makes extended functions available to the user, e.g. new rotors can
be used. A new version of the basic software is uploaded.

 Download current software versions from the website www.eppendorf-support.com.

 Install current software versions.
 To perform a software upgrade, EPROM may need to be replaced.
PS 06 Electrical safety check.
See chapter “Device tests for centrifuges”.
PS 07 Speed check.
See chapter “Device tests for centrifuges”.
PS 08 Timer check.
See chapter “Device tests for centrifuges”.
PS 09 Temperature check.
See chapter “Device tests for centrifuges”.
PS 10 Compulsory testing in Germany (BGR 500)
The accident prevention regulation BGR 500 (formerly VBG 7z) is valid in Germany.
Centrifuges with a rated power of > 500 W or a kinetic energy of > 10,000 Nm are subject to
testing.
The following Eppendorf AG centrifuges are subject to testing: Centrifuge 5804, 5804 R,
5810, 5810 R, 5810 RA, 5920 R.
These centrifuges must be tested once a year in their operating state and at least every 3
years in a dismantled state.
Rotors with a kinetic energy of ≤ 10,000 Nm are not subject to testing. Rotors that are used
in centrifuges subject to testing must be tested independent of their power output.
The owner of the centrifuge is obliged to document and keep the check results.
 Maintenance
Centrifuge 5418 R 59
English (EN)

9.4 Device tests for centrifuges

9.4.1 Speed check

Measuring device
• Digital hand tachometer suitable for contactless measurement, or stroboscope
• Measurement range: 1 to ≥ 30,000 rpm
• Accuracy: ≤ 0.1%
1. Install the rotor.
2. Fully load the rotor with filled tubes.
3. Mark the rotor hub with a reflector (measuring device accessory).
4. Close the centrifuge lid.
5. Start the centrifuge with the desired speed. Let the centrifuge run until the speed has stabilized.
6. Measure the speed via the monitoring glass in the centrifuge lid using the operating manual of the
measuring device.
7. Note the measuring result.
8. If required, repeat steps 5 to 7 for other rotational speeds.
A recommendation on the rotational speeds to be tested, and their tolerances, can be found in the technical
data or OQ of the corresponding centrifuge.

9.4.2 Temperature check

Ambient temperature: 23°C ± 2°C

Measuring device
• Thermometer with a temperature sensor and a low internal heat capacity, e.g. thermocouple, max. Ø
0.5 mm)
• Measurement range: 0 °C to ≥ 100 °C
• Accuracy: ≤ 0.1°C
Auxiliary equipment
• Rotor
• Tubes that fit the rotor, with min. volume of 1.5 mL

9.4.2.1 Refrigerated centrifuges

1. Install the rotor and load it symmetrically with tubes.

2. Fill the tubes with ethylene glycol.
3. Set the maximum rotational speed.
4. Set the minimum temperature that can be achieved (depends on the rotor).
5. Precool the centrifuge and rotor with tubes and with adapters, if required, using fast temp (refer to the
operating manual for the centrifuge).
6. Start the centrifuge with the previously set max. speed and min. temperature.
7. Wait for 60 min. Then stop the centrifuge.
8. Measure the temperature in the center of the tubes immediately after opening.
9. Compare the sample temperature with the temperature displayed by the centrifuge.
 Maintenance
60 Centrifuge 5418 R
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9.4.2.2 Non-refrigerated centrifuges

For non-refrigerated centrifuges, sample heating is checked against the ambient temperature.

1. Install the rotor and load it symmetrically with tubes.

2. Fill the tubes with ethylene glycol.
3. Set the maximum rotational speed.
4. Start the centrifuge and let it run for at least 45 min.
5. Measure the ambient temperature.
6. Stop the centrifuge and measure the temperature in the center of the tubes immediately after opening.
7. Compare the ambient temperature and sample temperature.

A recommendation on the temperatures to be tested, and their tolerances, can be found in the technical
data or OQ of the corresponding centrifuge.

9.4.3 Timer check

Measuring device
• Stopwatch
• Accuracy: ≤ 0.1 seconds
1. Set the run time to continuous operation.
2. Start the centrifuge and stopwatch at the same time.
3. Allow the centrifuge to run at least 5 min.
4. Compare the time displayed by the centrifuge with the time measured by the stopwatch.

A recommendation on the times to be tested, and their tolerances, can be found in the technical data or OQ
of the corresponding centrifuge.
 Maintenance
Centrifuge 5418 R 61
English (EN)

9.4.4 Electrical safety check

Effective standard
• Pre-IEC 62638 (international)
• VDE 701/702 (Germany)
• NFPA 99 (USA)

Notes concerning the measuring procedure:

• The electrical safety check may only be carried out by a suitably trained electrician.
• After repairs or after work on the electrical system of the centrifuge (incl. protective earth conductor
system) the electrical safety must be tested.
• The electrical safety must be checked at regular intervals (e.g. every 12 months).
• Measure on a grounded, metallic point that is not insulated and can be touched from the outside (e.g.
housing screws).
• The leakage current or resistance of the PE conductor cannot be measured at the centrifuge lid, rotor, or
motor shaft.
• Never measure at any interfaces that may be present. The centrifuge electronics could become
damaged.
• Due to the internal electrical design of the centrifuge, the insulation resistance cannot be measured
correctly.
• Due to the internal electrical design of the centrifuge, the equivalent leakage current cannot be
measured correctly.
→ Always measure the real leakage current (direct measurement or differential current measurement).
 Maintenance
62 Centrifuge 5418 R
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 Technical data
Centrifuge 5418 R 63
English (EN)

10 Technical data
10.1 Power supply

Tab. 10-1: Centrifuge 5418 R

Mains/power connection: 230 V, 50 to 60 Hz
120 V, 50 to 60 Hz
100 V, 50 to 60 Hz
Current consumption: 1.4 A (230 V)
2.8 A (120 V)
3.0 A (100 V)
Power consumption: max. 320 W
EMC: interference emission (radio interference) EN 61326 -1 – Class B (230 V)
FCC 15 Part A (120 V)
EMC: noise immunity EN 61326-1
Overvoltage category: II
Fuses: 2.5 AT (230 V)
6.25 AT (120 V / 100 V)

10.2 Ambient conditions

Environment: Only for use indoors.

Ambient temperature: Centrifuge 5418: 2 °C to 40 °C
Centrifuge 5418 R: 15 °C to 35 °C
Max. relative humidity: 75 %, non-condensing humidity.
Atmospheric pressure: Use up to an altitude of 2.000 m above MSL.
Degree of pollution: 2

10.3 Weight/dimensions

Tab. 10-2: Centrifuge 5418 R

Dimensions: Width: 298 cm (11,73 in)
Depth: 463 cm (18,23 in)
Height: 463 cm (18,23 in)
Weight without rotor: 22 kg
Noise level: < 57 dB (A) *

*) The noise level was measured according to DIN EN ISO 3745 frontally in a sound measuring room with
accuracy class 1 at a distance of 1 m from the device and at lab bench height.
 Technical data
64 Centrifuge 5418 R
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10.4 Application parameters

Start and stop times according to DIN 58 970
Rotor 5418 5418 R
Voltage Acceleration Deceleration Voltage Acceleration Deceleration
times times times times
FA-45-18-11 230 V 16 s 18 s 230 V 13 s 13 s
120 V 16 s 18 s 120 V 13 s 13 s
100 V 18 s 18 s 100 V 13 s 13 s

These values were calculated at 23 °C.

run time 0.5 min to 99 min, as well as infinity (oo),

Adjustable to 10 min in 0.5 min increments, then
increments of 1 min
Temperature 0°C to 40°C
Relative centrifugal force (RCF) 1 to 16,873 x g
adjustable to 5000 × g in 100 x g increments, then
increments of 200 × g.
RPM 100 to 14,000 rpm
adjustable in 100 rpm increments
Maximum load 18 micro test tubes of 2.0 mL each.
Max. kinetic energy 2,600 Nm
Test log mandatory: No
Allowable density of the centrifuge material (at 1.2 g/mL
maximum g-force (rcf)/speed (rpm) and maximum
load)

10.5 Tolerances

All data applies to an ambient temperature of 23 °C ± 2 °C.

Parameters Tolerance
Speed (1000 rpm up to max. speed) ± 100 rpm
Temperature At 6 °C ± 2 °C
Timer At 5 min run time ± 2 s
 Ordering information
Centrifuge 5418 R 65
English (EN)

11 Ordering information
11.1 Images of spare parts
Abb. 11-1: Centrifuge 5418 R

2-2

2-6

2-3

2-1
2-4

1-7

1-4

1-3

1-8 1-1

1-2

1-5

Fig. 11-1: Centrifuge 5418 R

 Ordering information
66 Centrifuge 5418 R
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Abb. 11-2: Internal view 1

3-4 6-2
3-5

3-1
6-1
5-1
5-2

1-6

4-7

Fig. 11-2: Internal view 1

Abb. 11-3: Internal view 2

5-4
5-3
3-3

7-1

7-2

Fig. 11-3: Internal view 2

 Ordering information
Centrifuge 5418 R 67
English (EN)

Abb. 11-4: Motor seal/temperature sensor

5-5

4-5

4-4

Fig. 11-4: Motor seal/temperature sensor

Abb. 11-5: Reed contact

3-2

Fig. 11-5: Reed contact

 Ordering information
68 Centrifuge 5418 R
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Abb. 11-6: PCB converter

Fig. 11-6: PCB converter

Abb. 11-7: Universal PCB control

Fig. 11-7: Universal PCB control

 Ordering information
Centrifuge 5418 R 69
English (EN)

11.2 Spare parts

11.2.1 Housing

Fig. Item Order no. Description

(International)
11-1 1-1 5401 850.025 Upper part of housing
11-1 1-2 5401 850.041 Bottom cover
11-1 1-3 5401 850.050 Keypad
Rotary knob
11-1 1-4 5702 852.040 1 piece
Device foot
4 pieces
11-1 1-5 5426 850.160 5415 R/5418 R
Vent cap ground plate
11-2 1-6 5404 850.069 3 pieces
11-1 1-7 5401 850.068 Sealing for chamber
11-1 1-8 5401 850.076 Tray for condensation water
--- 1-9 5404 850.018 Cap for emergency release
Cover cap
Set of 6 pieces
--- 1-10 5401 850.165 for housing screws

11.2.2 Lid

Fig. Item Order no. Description

(International)
Lid spring
11-1 2-1 5401 850.084 cpl. set
11-1 2-2 5401 850.033 Lid covering
11-1 2-3 5401 850.157 Lid panel with monitoring glass
11-1 2-4 5401 850.130 Lid axle
Guide sleeve
2 pieces
--- 2-5 5401 850.106 for axle
11-1 2-6 5702 853.046 Reed magnet
 Ordering information
70 Centrifuge 5418 R
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11.2.3 Lid latch

Fig. Item Order no. Description

(International)
11-2 3-1 5401 850.149 Motor for lid latch
11-5 3-2 5426 853.126 Reed contact
Microswitch lid latch
11-3 3-3 5810 853.044 with actuator
Latch hook
11-2 3-4 5426 853.240 2 pieces
Tension spring
11-2 3-5 5418 850.320 Set of 2

11.2.4 Electronics

Fig. Item Order no. Description

(International)
11-7 4-1 5401 850.092 PCB Control
PCB Converter
11-6 4-2 5426 851.220 230 V
11-6 4-2 5426 851.239 120 V
11-6 4-2 5426 851.247 100 V
Heat sink
for PCB Converter
--- 4-3 5426 851.069 100 V
Rubber sleeve
11-4 4-4 5426 855.072 for temperature sensor
Temperature sensor
11-4 4-5 5811 643.000 rotor bowl
--- 4-6 5408 856.070 condenser
Mains input module
11-2 4-7 5426 856.028 100/120 V
11-2 4-7 5426 856.117 230 V
Fuse
--- 4-8 5425 351.003 2.5 A T (230 V), set of 2
--- 4-8 5426 355.100 6.25 AT (100 V/120 V), set of 2
 Ordering information
Centrifuge 5418 R 71
English (EN)

11.2.5 Drive up to serial number 04823

Fig. Item Order no. Description

(International)
Motor
11-2 5-1 5401 850.114 100/120/230 V
Motor suspension
11-2 5-2 5425 300.107 Set of 3
Brake resistor
11-3 5-3 5426 856.125 100 V
11-3 5-3 5426 856.095 120/230 V
11-3 5-4 5426 856.044 Temperature monitor
Motor seal
11-4 5-5 5426 850.135 rubber sleeve

11.2.6 Drive from serial number 04824

Fig. Item Order no. Description

(International)
Motor
11-2 5-1 5401 850.114 100/120/230 V
Motor suspension
11-2 5-2 5425 300.107 Set of 3
Brake resistor
11-3 5-3 5401 850.190 100/120/230V
Motor seal
11-4 5-5 5426 850.135 rubber sleeve

11.2.7 Fan

Fig. Item Order no. Description

(International)
Condenser fan
11-2 6-1 5426 854.076 100/120 V
11-2 6-1 5426 854.068 230 V
Compressor fan
11-2 6-2 5426 854.114 100/120 V
11-2 6-2 5426 854.106 230 V
 Ordering information
72 Centrifuge 5418 R
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11.2.8 Cooling

Fig. Item Order no. Description

(International)
Compressor
11-3 7-1 5703 852.132 100-230 V, 50-60 Hz
11-3 7-2 5408 855.138 Dryer cartridge

11.2.9 Accessories

Fig. Item Order no. Description

(International)
--- 8-1 5401 850.122 Packing
Rotor key
--- 8-2 5416 301.001 Standard
Rotor FA-45-18-11
--- 8-3 5418 707.005 aerosol-tight, angle 45°, 18 places, max. tube diameter 11 mm, incl.
rotor lid (aluminum)
Rotor lid for FA-45-18-11
--- 8-4 5418 708.001 aerosol-tight, aluminum
Seal for rotor lid
FA-45-18-11 (5418/5418 R), FA-45-6-30 (5804/5804 R/5810/5810 R),
FA-6×50 (5920 R)
--- 8-5 5418 709.008 5 pieces
 Ordering information
Centrifuge 5418 R 73
English (EN)

11.3 Suggested spare parts

We recommend the following spare parts for the initial equipment of your service stock:

Fig. Item Order no. Description

(International)
Motor
11-2 3-1 5426 853.304 for lid latch
11-2 5-1 5401 850.114 100/120/230 V
11-7 4-1 5401 850.092 PCB Control
PCB Converter
11-6 4-2 5426 851.220 230 V
11-6 4-2 5426 851.239 120 V
11-6 4-2 5426 851.247 100 V
Motor suspension
11-2 5-2 5425 300.107 Set of 3
Brake resistor
11-3 5-3 5426 856.125 100 V
11-3 5-3 5426 856.095 120/230 V
11-3 5-3 5401 850.190 100/120/230V
 Ordering information
74 Centrifuge 5418 R
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 Technical information
Centrifuge 5418 R 75
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12 Technical information
 Technical information
76 Centrifuge 5418 R
English (EN)
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