Bioprocess Catalog

Eppendorf Bioprocess Products

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»We help bring life-saving

treatments to the world.«
Lina Tao - Head of Bioprocess Unit, Eppendorf SE

Bioprocess engineers develop and produce a multitude of products for more than a decade and with our single-use stirred-tank bioreactor
and ingredients available today. Their applications are diverse and technology, we are aiming to provide the new gold-standard for this
the products can be found in the pharmaceutical, chemical, and growing industry. By exploiting the strong synergies in bioreactor
nutritional industry. Within those branches, promising new bioprocess technology and polymer manufacturing, Eppendorf bioprocess has
approaches, such as cell and gene therapies (CGT) or stem cell-derived emerged as a global player and valuable resource to its customers.
novel food, start gaining momentum and open the door towards a With our equipment, training programs, and application services, we
new tomorrow. The progresses in the CGT field hold great potential to support scientists in resolving cultivation bottlenecks from bioprocess
revolutionize how we will live in the future, but theses progresses will development through production. In that way and in line with our
only be possible with the help of the bioprocessing industry. corporate mission, we contribute to the efforts of our customers
With our Eppendorf Bioprocess solutions, we are committed to help worldwide to improve human living conditions by bringing life-saving
bring life-saving treatments to the world. Our bioprocess solutions treatments to the world.
are successfully used in the development of cell and gene therapies
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Table of contents
SYSTEMS 12 - 51
>> DASbox® Mini Bioreactor System 18 - 21
>> DASGIP® Parallel Bioreactor Systems 22 - 25
>> Bioprocess Autosampler 26 - 27
>> SciVario® twin 28 - 31
>> BioFlo® 120 32 - 37
>> BioFlo® 320 38 - 43
>> BioFlo® 720 44 - 45
>> BioFlo®/CelliGen 510 46 - 47
>> BioFlo® 610 48 - 49
>> BioFlo® Pro 50 - 51

BIOREACTORS 52 - 107
>> BioBLU® c Single-Use Bioreactors 58 - 61
>> BioBLU® f Single-Use Bioreactors 62 - 65
>> BioBLU® Single-Use Bioreactors Adaptor Kits 66 - 67
>> DASbox® Mini Bioreactor 68 - 69
>> DASGIP® Bioblock / SciVario® twin Spinner Vessels 70 - 71
>> DASGIP® Bioblock / SciVario® twin Stirrer Vessels 72 - 73
>> SciVario® twin Benchtop Bioreactors for Cell Culture 74 - 75
>> SciVario® twin Benchtop Bioreactors for Microbiology 76 - 77
>> DASbox®, DASGIP®, and SciVario® twin Bioreactor Accessories 78 - 89
>> BioFlo® 120 Bioreactors 90 - 95
>> BioFlo® 120 Bioreactors Accessories 96 - 97
>> BioFlo® 320 Bioreactors 98 - 103
>> BioFlo® 320 Bioreactors Accessories 104 - 105
>> BioFlo®/CelliGen Bioreactors Replacement Parts 106 - 107

SOFTWARE 108 - 119

>> BioCommand® SCADA Software 112 - 113
>> DASware® control 114 - 115
>> DASware® connect 116
>> DASware® analyze 117
>> DASware® design 118 - 119

MONITORING & CONTROL 120 - 135

>>  ASGIP® PHPO for Monitoring of pH, DO, Redox and/or Level
D 124 - 125
>> DASGIP® GA for Exhaust Analysis 126 - 127
>> DASGIP® OD4 for Optical Density Monitoring 128
>> DASGIP® MP8 and MP4 Multi Pump Modules 129
>> DASGIP® TC4SC4 for Temperature and Agitation Control 130 - 131
>> DASGIP® Bioblock 132
>> DASGIP® Rotameters and Gassing Modules 133
>> DASGIP® MX Modules for TMFC Gas Mixing 134 - 135
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SYSTEM ACCESSORIES 136 - 171

>> Fibra-Cel® Disks 138 - 139
>> System Accessories 140 - 147
>> DASGIP® EGC for Exhaust Condensation 148
>> DASGIP® Peltier Exhaust Condensers 149
>> DASGIP® Feeding Accessories 150 - 151
>> DO Sensors 152 - 157
>> pH Sensors 158 - 163
>> Redox Sensors 164 - 168
>> Turbidity Sensors 169
>> CO2 Sensors 169
>> Optical Density Sensors 170
>> Level Sensors 171
>> Temperature Sensors 171

SERVICE 172 - 217

>> DASbox® Mini Bioreactor System Performance Plans 174 - 175
>> DASGIP® PHPO and OD4 Performance Plans 176 - 177
>> DASGIP® GA Performance Plans 178 - 179
>> DASGIP® TC4SC4 Performance Plans 180 - 181
>> DASGIP® MP8 and MP4 Performance Plans 182 - 183
>> DASGIP® MX4/4 Performance Plans 184 - 185
>> DASGIP® MX4/1, MF4 and Rotameters Performance Plans 186 - 187
>> DASGIP® PBR4 Performance Plans 188 - 189
>> DASGIP® Control System Performance Plans 190 - 191
>> SciVario® twin Performance Plans 192 - 193
>> DASGIP®, DASbox®, and SciVario® twin Vessels Performance Plans 194 - 195
>> BioFlo® 120 Performance Plans 196 - 197
>> BioFlo®/CelliGen 115 Performance Plans 198 - 199
>> BioFlo® 320 Performance Plans 200 - 201
>> BioFlo®/CelliGen 310 Performance Plans 202 - 203
>> CelliGen BLU Performance Plans 204 - 205
>> BioFlo® 415 Performance Plans 206 - 207
>> BioFlo®/CelliGen 510 (RPC/AB) Performance Plans 208 - 209
>> BioFlo® 610 Performance Plans 210 - 211
>> BioFlo® 720 Performance Plans 212 - 213
>> BioFlo® Pro Performance Plans 214 - 215
>> CelliGen Pro Performance Plans 216 - 217

Are you looking for more Details?

www
Do you miss further information or simply wish additional details?

Application Videos Websites On our catalog pages you will find QR codes with icons showing you
Notes what kind of materials (brochure, website, 360 degree views and
much more) we offer for viewing or downloading.

Simply scan the QR Code on the corresponding pages and make use
of our online offers.
6

epServices
for premium performance

Eppendorf Services
At Eppendorf Services, we are committed to providing sincere, reliable services and tools to help you maintain premium performance, and
maximum safety of your Eppendorf instruments and your workflows. This includes a comprehensive range of carefully designed service
solutions performed by our dedicated bioprocess service teams worldwide.

With our services, we complement our bioprocess portfolio with Technical and Application Support, Training, and Maintenance Services.
 7

Supporting You

Application Support Seminars and Technical Support Maintenance and

Training Certification

Knowledge Base Technical and Application Support

A comprehensive collection of bioprocess-related application notes, You can expect very high standards of support for your products
FAQs, and videos can be found on our website under Knowledge and applications from Eppendorf. Our team of specialists is pleased
Base and Applications. to help you with advice and assistance for all kinds of questions
regarding our bioprocess products and their applications.

Bioprocess Performance Plans

Technologically demanding products require first-class service Eppendorf Training Center
to ensure that the results they produce are optimized. Customers In addition to support during installation, we highly recommend
can rely on Eppendorf Service for superior support for their training for all new bioprocess customers. Furthermore Eppendorf
bioprocess products, beginning with startup of the system. The also offers individual training adapted to the user’s requirements
services range from technical support and troubleshooting to like training of new employees or advanced/refresher training for
delivery of replacement parts on short notice and customer-specific employees already having experience with the bioprocess systems
maintenance programs. in place. The structure and methods of the training can be tailored
to the customer’s requirements from a general overview to a very
detailed session on specific products or issues. Training in small
Preventive Maintenance groups makes it possible to provide individual guidance.
As with all complex technical systems, Eppendorf bioprocess
equipment should be maintained regularly to keep all parts in good www.eppendorf.link/epServices
working order. This maintenance avoids cost-intensive down times
and contributes to preservation of value. We recommend a complete www
preventive maintenance once a year. Additionally, we encourage
users to execute certain maintenance actions prior to every run or in
regular cycles (e.g. every month). We will be happy to advice.
8

Our Bioprocessing Solutions for

Your Applications: Discover our
Website www www.eppendorf.link/bioprocess

Information on bioprocessing is now available in a central location: Find research examples, application notes, and white papers on topics like
bioprocess development, stem cell cultivation, scale-up, and many more. Get to know our customers working in biopharmaceutical, biochemical,
and food/feed laboratories. Get engaged in webinars, see our training offerings and do not miss any news and events related to bioprocessing.

Vaccines Antibodies/hormones Stem cells

There is a rising demand for development Improved cultivation techniques such as Stem cell-based technologies are promising
of new vaccines. Eppendorf bioprocess perfusion and new possibilities in data approaches for therapy and drug discovery.
equipment helps companies in setting up handling and automation enhance develop- For commercialization, researchers are
flexible processes, optimized to balance ment of antibodies, therapeutic proteins, evaluating standardization of their cultures
costs and time-to-market. and active ingredients. and efficient scale-up.

Eppendorf Digital
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The Eppendorf App with its new splendid design is based on the latest technology to
provide better usability. Have more fun browsing through the product catalog to find
the right product for you. Start with the many possibilities to get information about
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function, it is now even easier to place products virtually in your lab. Experience
the diversity of Eppendorf on a 1:1 scale. Plan space requirements and configure
accessories comfortably from your tablet or smartphone.

Here are some more highlights you will find in our app:
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racks.

Download the Eppendorf App! We wish you a lot of fun!

* To use the AR function, your device needs the operating system IOS 13.0 or higher.
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Bioprocess
Knowledge Hub
Whether you are just starting in bioprocessing or have
numerous years of experience in the field, it can be a vast area
with many aspects to consider. Find information on general
bioprocessing topics like bioprocess monitoring and control
and scale-up, as well as expert advice on how to optimize
bioprocessing for specific applications, including cell culture
and microbial fermentation.

Become an expert in bioprocessing.

Join us at www.eppendorf.link/bioprocess-basics

www.eppendorf.link/bioprocess-basics
www
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Recent Application Notes

Resolving Cultivation Bottlenecks: The Bioprocessing Journey

Bioprocessing relies on living cells or their components to create pharmaceutical, biofuel, or nutrition
products. Whether bacterial, fungal, plant or mammalian, cells used for these processes need unique
conditions for optimal growth and product formation. This eBook describes the advantages of using
bioreactors and highlights different aspects that need to be considered when working with a bioreactor.
RESOLVING
CULTIVATION
BOTTLENECKS:
THE BIOPROCESSING
JOURNEY

Page 3 Page 4 Page 5 Page 6

The Next Level: Why
Move to a Bioreactor
How Do Bioreactors
Boost Reproducibility?
A Balanced Diet: Feeding
Bioreactor Cultures
The Basic Components of
a Bioreactor www.eppendorf.link/publicationBP2020

From Shaker to Pilot/Production Bioreactor: How Scale Up Assist Using the BioFlo® 720 Bioreactor
Control System Can Help Your Antibody Production Workflow. APPLICATION NOTE No. 436

From Shaker to Pilot/Production Bioreactor:

How Scale Up Assist Using the BioFlo® 720
Bioreactor Control System Can Help Your Antibody
This study details the use of this new Scale Up Assist feature of the BioFlo 720 Bioreactor Control Production Workflow.
Amanda Suttle, John Longsworth, and Ma Sha
Eppendorf, Inc., Enfield, CT, USA

System to scale CHO batch cultures from 3 L to 10 L BioBLU® Single-Use Bioreactors and from the 10 L to
Contact: bioprocess-experts@eppendorf.com

Abstract

To scale upstream bioprocesses, an effective scale-up points to use for different vessel sizes.

Thermo ScientificTM HyPerformaTM 50 L single use bioreactor. Our results demonstrated that the
strategy is required to ensure the reproducibility of both We cultivated Chinese Hamster Ovary cells (CHO) using
cell culture growth and batch yields at large working BioBLU Single-Use Vessels and a Thermo ScientificTM
volumes, ideally with little or no additional optimization. HyPerformaTM 50 L SUB. Our results demonstrated that
The Constant Power approach is a successful method for the parameters provided by the new Scale Up Assist
matching bioreactor yields at different scales. This study software resulted in similar cell growth and monoclonal

parameters provided by the new Scale Up Assist software resulted in similar cell growth and monoclonal
details the use of this new Scale Up Assist feature of the antibody (mAb) production yields for all three vessel sizes.
BioFlo 720 Bioreactor Control System to scale CHO batch Adoption of the controller’s Scale Up Assist function can
cultures from 3 L to 10 L BioBLU® Single-Use Vessels and decrease process development time while also helping
from the 10 L to 50 L Single Use Bioreactor (SUB) bag. to ensure consistent mAb yields across vessel sizes and
Using a built-in constant power software algorithm, the bioreactor platforms.

antibody (mAb) production yields for all three vessel sizes. Adoption of the controller’s Scale Up Assist
Scale Up Assist specified what agitation and gassing set

Introduction

function can decrease process development time while also helping to ensure consistent mAb yields Bioprocess development is often done at small or bench
scale to save production costs, optimize ideal conditions for
cultures, and shorten time to market.
include keeping a constant tip speed across bioreactors,
matching oxygen volumetric mass transfer coefficients (KLa),
and maintaining constant impeller power per unit volume
An effective strategy is required during scale-up to ensure (P/V). We have utilized the Constant Power approach,

across vessel sizes and bioreactor platforms.

matching cell growth in cultures and similar mAb or other manually calculated, for scale-up in both fermentation [1]
protein production yields from small, bench, and pilot scale. and cell culture [10] applications. This method involves
However, sometimes these strategies to scale-up cell culture calculating impeller power consumption per unit volume
processes are not as straightforward as we would like. using Equation 1:

www.eppendorf.link/appnote436
Many strategies have been established to streamline the
scale-up of protein and mAb production. These strategies P/V = Nρ × ρ × N3 × d

Scale-Up of a Biosimilar Production Process with CHO Cells from Small to Bench Scale
APPLICATION NOTE No. 416

Scale-Up of a Biosimilar Production Process with CHO

Cells from Small to Bench Scale
Franziska Janke, UGA Biopharma GmbH, Neuendorfstraße 20a, 16761 Hennigsdorf, Germany
Lars Kober, UGA Biopharma GmbH, Neuendorfstraße 20a, 16761 Hennigsdorf, Germany
Robert Glaser, Eppendorf Bioprocess Center, Rudolf-Schulten-Str. 5, 52428 Juelich, Germany
Bioprocess scale-up from small development scale to pilot and production scale is a fundamental part
of process development in the biopharmaceutical industry. To reproduce process performance while
Contact: bioprocess-experts@eppendorf.com; info@ugabiopharma.com

Abstract

Bioprocess scale-up from small development scale

to pilot and production scale is a fundamental part of
process development in the biopharmaceutical industry.
To reproduce process performance while increasing
aspects of scale‐up are oxygen mass transfer and the
dissolved oxygen (DO) concentration in the cultivation
medium. Oxygen mass transfer depends mainly on the air
sparge system, the gassing rate, oxygen concentration,
increasing working volume, the optimal cellular environment must be replicated. In this study, we
scaled up a CHO cell culture bioprocess from 1 L using a DASGIP® Parallel Bioreactor System to 5 L
working volume, the optimal cellular environment must be selected impellers, their settings, power input and
replicated. properties for gas dispersion.
Scale-up often comes along with transference of the In this study, we scaled up a CHO cell culture
process to bioprocess systems with other properties, bioprocess from 1 L using a DASGIP® Parallel Bioreactor
such as different geometries and engineering parameters. System to 5 L using a BioFlo® 320 Bioprocess Control

using a BioFlo® 320 Bioprocess Control Station. To develop a scale-up strategy, we partly compared
Depending on the scale-up strategy, parameters such as Station. To develop a scale-up strategy, we partly
mixing time, oxygen transfer, and power input are to be compared power number and volumetric mass transfer
considered. As it is usually not feasible to conserve all the coefficient (kLa) of the bioreactors. The results show that
parameters when scaling up, bioengineers must assess kLa value can be used as a suitable scale-up criterion from
which are the most critical. Among the most important small to bench-scale.

power number and volumetric mass transfer coefficient (kLa) of the bioreactors. The results show that
kLa value can be used as a suitable scale-up criterion from small to bench-scale.
Introduction

Monoclonal antibodies and therapeutic proteins are an In the development of biosimilars, small bioreactors save
important segment for the biotech industry. The execution of time, material, and resources. With the ability to work in
a successful bioprocess scale-up and transfer of technology parallel, the DASGIP Parallel Bioreactor System equipped
and activities enables a fast time to market approach, with 1 L vessels can be used to optimize bioprocess
supply chain risk mitigations, and market access [1]. Also, parameters, including temperature, shear stress, and
from a regulatory perspective, the Quality by Design (QbD) pH regulation. In the case of large scale production, it is
approach that is promoted by the EMA and FDA requires important to show that the results of the parallel system can
detailed process and product understanding gained by a be transferred to larger scales, such as the Eppendorf 5 L

www.eppendorf.link/appnote416
successful scale-up as well as a clear definition of critical vessel of the BioFlo 320 system. Another advantage is that
quality attributes and critical process parameters. the higher harvest volume of the larger system provides

Parallel Escherichia coli fermentation in the SciVario® twin, the Flexible Controller for All Your
Bioprocess Needs Application Note | No. 433 | 2021

Parallel Escherichia coli fermentation in the

SciVario® twin, the Flexible Controller for All
Your Bioprocess Needs
We performed parallel batch and fed-batch fermentations of Escherichia coli (E. coli) with the SciVario Robert Glaser1, Anne Niehus1, and Ma Sha2
1

2
Eppendorf Bioprocess Center, Juelich, Germany
Eppendorf, Inc., Enfield, CT, USA

twin using Eppendorf 1 L and 3 L glass vessels. These experiments highlighted SciVario twin’s
Contact: bioprocess-experts@eppendorf.com

Abstract

capabilities to control complex processes using various vessel sizes. In this application note, we The range of experimental organisms available for bio-
processing protocols and the complex demands for their
cultivation require new options in advanced bioprocess
In this application note, we describe major steps of a
typical E. coli fermentation, starting from the preparation
of the inoculum to the setup of process parameters and
control systems. The SciVario® twin bioprocess controller control strategies and operation of the vessels. We further

describe major steps of a typical E. coli fermentation, starting from the preparation of the inoculum to
can be used to regulate both the microbiological param- describe in detail the preparation of the fermentation
eters and in vitro cell culture needs of these choices, as medium, the bioprocess run itself and the handling of the
well as the application of single-use vessels using only a SciVario twin controller.
single controller platform. We propose this application note as a starting point for
We performed parallel batch and fed-batch fermenta- further optimization of our bioprocess control systems.

the setup of process parameters and control strategies and operation of the vessels. The application tions of Escherichia coli (E. coli) with the SciVario twin
using Eppendorf 1 L and 3 L glass vessels. These experi-
ments highlighted SciVario twin’s capabilities to control
complex processes using various vessel sizes.
We used E. coli K12 W3110 (DSM 5911) in this study.
The application note can serve as a starting point for
further optimization.

note can serve as a starting point for further optimization.

www.eppendorf.link/appnote433
Fig. 1: SciVario twin parallel bioprocess control system for glas and single-use bioreactors and fermenter.
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Controlled, Large-Scale Manufacturing of hiPSC-Derived Cardiomyocytes in ­

Stirred-Tank Bioreactors
APPLICATION NOTE No. 409 I April 2019

Controlled, Large-Scale Manufacturing of hiPSC-Derived

Cardiomyocytes in Stirred-Tank Bioreactors
Yang Jiang1, Karin Langenberg1, Viola Borgdorff1, Marta Duńska1, Robert Post1, Oscar Bartulos1, Mieke Doornbos1, Stefan Braam1,
Arie Reijerkerk1

Effective drug discovery and development relies in large part on the availability of predictive preclinical
1
Ncardia BV, Leiden, The Netherlands; 2Ncardia Inc, Wayne, PA USA; Contact: arie.reijerkerk@ncardia.com
Ulrike Rasche³
³Eppendorf Bioprocess Center Juelich, Germany; Contact: rasche.u@eppendorf.com

model systems. Application of human cellular models from tissues which are difficult to access, such
Abstract

Effective drug discovery and development relies in They expanded hiPSCs as cell aggregates, in a
large part on the availability of predictive preclinical DASbox® Mini Bioreactor System. Aggregate size, hiPSC-
model systems. Application of human cellular models marker expression during the expansion phase, and

as cardiomyocytes and neurons, is still challenging. Technologies based on human induced pluripotent
from tissues which are difficult to access, such as differentiation to the desired cell type were reproducible
cardiomyocytes and neurons, is still challenging. in three batches. In a proof of concept study, the
Technologies based on human induced pluripotent researchers scaled-up the process using a BioFlo® 320
stem cells (hiPSC) hold great promise to overcome this bioprocess control system. The cells retained key
challenge. Their routine use in industrial drug research iPSC markers during the expansion phase, providing

stem cells (hiPSC) hold great promise to overcome this challenge. This application note describes how
requires a constant supply of stem cell-derived cells confidence that stirred-tank bioreactors are suitable to
of consistent high quality. Researchers from Ncardia® scale-up the production of hiPSC-derived cellular models.
developed a bioprocess for the large-scale manufacturing The production of several billion cardiomyocytes
of cardiomyocytes derived from a variety of healthy per batch will enable the screening of large libraries
and diseased hiPSC lines for implementation into their of compounds against phenotypic cellular models

researchers from Ncardia® expanded hiPSCs as cell aggregates ina a DASbox® Mini Bioreactor System.
DiscoverHIT platform. representing human biology.

In a proof-of-concept study, the researchers scaled-up the process using a BioFlo 320 bioprocess
Introduction

Human stem cell technologies enable an end-to-end solution

for efficient high-throughput phenotypic screening in
drug discovery and development. State-of-the-art hiPSC

controller.
technologies allow the generation of relevant human cell
models for diseases derived from virtually any patient. Ncardia is a stem cell drug discovery and development
HiPSC-based cellular assays for drug research need to be company developing highly predictive human cellular (disease)
robust and reliable, and compatible with automated high assay systems for drug discovery. Its DiscoverHIT is a full
throughput screening (HTS) platforms. To mass produce

www.eppendorf.link/appnote409
service HTS-screening solution based on human induced
such assays, hiPSCs need to be expanded and differentiated pluripotent stem cell technology, comprising disease model
into the desired cell type in a reproducible and scalable development, manufacturing, assay development, and high
manner. throughput screening [1].
Stem cell characteristics and differentiation are

Optimizing CD4+ T Cells Long-term Expansion Process in the DASbox® Mini Bioreactor System
APPLICATION NOTE No. 453

Optimizing CD4 + T Cells Long-term Expansion Process

T cell lymphocytes play a central role in the adaptive immune response. They are an essential tool of in the DASbox® Mini Bioreactor System: Impact of the
Dissolved Oxygen

adoptive cell therapy for the treatment of chronic viral infections and malignant diseases. However, the
Silvia Tejerina Vargas, Vincent Dufey, Aurélie Tacheny, Françoise De Longueville
Eppendorf Application Technologies S.A., Namur, Belgium
Contact: bioprocess-experts@eppendorf.com

development of cell-based therapy products generally requires the production of a large quantity of high- Abstract

T cell lymphocytes play a central role in the adaptive impact of different oxygen tensions on cell proliferation.

quality viable T cells in a controlled environment. In this study, we tested the suitability of BioBLU® 0.3c
immune response. They are an essential tool of adoptive The control of several growth parameters during cell
cell therapy for the treatment of chronic viral infections expansion resulted in an efficient proliferation of highly
and malignant diseases. However, the development viable and functional CD4+ T cells after 16 days of culture.
of cell-based therapy products generally requires the Given that 100% dissolved oxygen (DO) in the bioreactor
production of a large quantity of high-quality viable T cells corresponds to 20.9% atmospheric oxygen level,

Single-Use Bioreactors controlled by a DASbox Mini Bioreactor System in the long-term expansion of
in a controlled environment. Stirred-tank bioreactors can incubation at two different oxygen tensions (70% or 20%
offer a suitable environment for the culture of T cells by DO) was carried out. The results suggested a tendency
providing homogeneous distribution of nutrients and towards a positive impact of lower DO levels on CD4+
gases, along with the maintenance of cells and molecules T cell proliferation rates. These results demonstrate the
in suspension with a high process control capability. In this substantial potential of the DASbox Mini Bioreactor System

CD4+ T cells as well as the impact of different oxygen tensions on cell proliferation.
study, we tested the suitability of BioBLU® 0.3c Single-Use used in combination with BioBLU 0.3c Single-Use
Bioreactors controlled by a DASbox Mini Bioreactor System Bioreactors to optimize T cell culture conditions.
in the long-term expansion of CD4+ T cells as well as the

Introduction

Adoptive cell therapy (ACT), a branch of immunotherapy, In clinical trials of various cancer treatments, adoptive cell
offers a promising treatment for chronic viral infections therapy with tumor infiltrating lymphocytes (TIL), gene-
and malignant diseases such as cancer [1]. This innovative modified T cells expressing novel T cell receptors (TCR),
approach involves the autologous or allogenic transplant or chimeric antigen receptors (CAR) have shown favorable
of immune cells into the patient’s body. Specifically, T cells results in the eradication of different types of tumors.

www.eppendorf.link/appnote453
are extracted from the patient, modified genetically (if Other therapies in development include Cytokine-Induced
necessary), expanded ex vivo and reinfused into the patient Killer (CIK) cells, γδ T cells, Regulatory T (Treg) cells, and
to target viral or tumor antigens [2,3]. Natural Killer (NK) cells [4]. At this time, five adoptive cell

Isolation of Exosomes from Stem Cells Using Single-Use Bioreactors, High-Speed- and
Ultracentrifugation
APPLICATION NOTE No. 476

Fast and Efficient Isolation of Exosomes from Stem Cells

Using a Combination of Single-Use Bioreactors,
High-Speed- and Ultracentrifugation

Cells release diverse types of membrane vesicles in the extracellular environment. Exosomes are
Pascal Rowart1, Vincent Dufey¹, Jan Knop², Françoise De Longueville¹
¹ Eppendorf Application Technologies S.A., Namur, Belgium
² Eppendorf SE, Hamburg, Germany
Corresponding author: rowart.p@eppendorf-eat.be
Additional contact: bioprocess-experts@eppendorf.com

Abstract
relatively small extracellular membrane vesicles (30–150 nm) and known as an important mode of
Cells release diverse types of membrane vesicles in the
extracellular environment. Those are called extracellular
vesicles (EVs) and comprise exosomes and microvesicles.
Exosomes are relatively small extracellular membrane
of exosomes. Subsequent clearance of the conditioned
culture medium was carried out by the Centrifuge CR22N,
a high-speed centrifuge able to reach a maximum speed of
32,300 × g. Here, the fixed-angle Rotor R15A was used due
cell-to-cell communication by transferring biomolecules, such as nucleic acids, proteins, enzymes, and
lipids, between cells. Here, we describe a fast and easy isolation process of exosomes from adipose-
vesicles (30–150 nm) and known as an important mode of to its high capacity of 10 × 50 mL and 10 × 15 mL conical
cell-to-cell communication by transferring biomolecules, tubes at once. In order to concentrate the exosomes
such as nucleic acids, proteins, enzymes, and lipids, further, the Ultracentrifuge CP100NX with a top speed
between cells. In addition, they can be used as biomarkers of 803,000 × g was used in concert with the swing-
for various diseases and are also investigated as natural bucket Rotor P32ST, which is able to hold up to 6 × 40 mL

derived stem cells by a combination of high-speed and ultracentrifugation. Stem cells were cultured in
drug delivery vehicles system for next-generation tubes. Furthermore, the use of a sucrose cushion during
therapeutic agents. the ultracentrifugation step allowed a rapid isolation of
Here, we describe a fast and easy isolation process pure and homogeneous exosomes compared to a more
of exosomes from adipose-derived stem cells by a heterogenous exosome population without sucrose.
combination of high-speed and ultracentrifugation. The The high-volume capacities of the utilized rotors reduced

BioBLU® 0.3c Single-Use Bioreactors and controlled by the DASbox® Mini Bioreactor System.
cells were cultured in BioBLU® 0.3c Single-Use Bioreactors the number of repetitive centrifugation steps and therefore
and controlled by the DASbox® Mini Bioreactor System. the exosome isolation time to less than 4 hours, which is
The DASbox Mini Bioreactor System allowed high quicker than other isolation methods, e.g. precipitation or
quantity stem cell culture and therefore high production filtration.

Introduction

www.eppendorf.link/appnote476
Cell signaling or cell communication is the ability of cells development and function of tissues [1]. This cell-to-cell
to receive, process, and transmit signals from or to their communication is achieved by direct cell interactions or
environment. It is a necessary process for the appropriate by the secretion of soluble factors. Most eukaryotic cells

Adeno-associated Virus Production in Suspension Cell Culture Using the SciVario® twin Bioprocess
Controller APPLICATION NOTE No. 450 | 2022

Adeno-associated Virus Production in Suspension Cell

Culture Using the SciVario twin Bioprocess Controller

Cell culture produced adeno-associated virus (AAV) vectors have gained momentum as one of the most
Jorge L. Escobar Ivirico and Ma Sha
Eppendorf, Inc., Enfield, CT, USA

Contact: bioprocess-experts@eppendorf.com

effective gene & protein delivery tools in vaccine production as well as gene therapy. In this application
Abstract

note, we demonstrated the feasibility of an efficient and scalable AAV production platform based on Vaccines are essential tools for resisting pathogens and
controlling global pandemics. Various types of virus
vaccines, such as live attenuated, inactivated pathogen
effective gene & protein delivery tools in vaccine produc-
tion as well as gene therapy.
In this application note, we demonstrated the feasibility of

suspension cell culture. We used a suspension-adapted HEK293 cell line as the host and incorporated a
and subunit vaccines have been widely produced using an efficient and scalable AAV production platform based
mammalian cell culture. However, they often rely on on suspension cell culture. We used a suspension-adapted
attachment cell culture. For higher production capacity, HEK293 cell line (Expi293F) as the host and incorporated
increased yield, and most importantly, ease of produc- a Helper-Free AAV System to eliminate the requirement
tion scale-up, it is more desirable to produce vaccines in for wild-type adenovirus co-infection. To achieve high

Helper-Free AAV System to eliminate the requirement for wild-type adenovirus co-infection.
suspension cell culture platforms. The widely available yield AAV capsid production, we performed the cellular
suspension bioreactors used in antibody production will transfection in a BioBLU 1c Single-Use Bioreactor con-
allow a faster development process of versatile vaccine trolled by the SciVario twin Bioreactor Control System.
platforms. Novel technologies such as mRNA vaccines, We monitored and analyzed metabolites, cell density and
Adeno virus vector and Adeno-associated virus (AAV) vec- viability, reaching a robust AAV titer of around
tor-based vaccines have all played a key role in controlling 1012 capsids/mL in line with the vaccine industry’s typical
the recent COVID-19 pandemic. Cell culture produced yields [1].
AAV vectors have gained momentum as one of the most

Interested in more bioprocess information?

Our quarterly Bioprocess Spotlight newsletter keeps you up to date about educational

www.eppendorf.link/appnote450
material, events, and product news related to your cell culture bioprocess workflow.
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12

Systems

Solutions that grow with you

From the parallel mini bioreactor system for early stage bioprocess development, the benchtop and parallel

bioreactor systems for the laboratory scale to the sterilize-in-place solutions for production: Eppendorf offers

users from industry and research extensive bioprocess solutions from a single source and meets the highest

quality demands.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 13

Systems

>> DASbox® Mini Bioreactor System 18 - 21

>> DASGIP® Parallel Bioreactor Systems 22 - 25
>> Bioprocess Autosampler 26 - 27
>> SciVario® twin 28 - 31
>> BioFlo® 120 32 - 37
>> BioFlo® 320 38 - 43
>> BioFlo® 720 44 - 45
>> BioFlo®/CelliGen 510 46 - 47
>> BioFlo® 610 48 - 49
>> BioFlo® Pro 50 - 51

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
14

Our Portfolio – Your Choice

Model DASbox® Mini Bioreactor System DASGIP® Parallel Bioreactor Systems

Page 18 22
Working volume ranges1) 60 – 250 mL 0.2 – 1.8 L
Single-use vessels  

Glass vessels, autoclavable  

Stainless-steel vessels, SIP

Interchangeable vessels  

Bacteria/yeasts/fungi  

Mammalian/animal cells  

Stem cells  

Insect cells  

Number of parallel units Up to 24 Up to 16

Controller2) DASware control DASware control
BioCommand®
DASware®  

Gas mixing options 4-gas (air, N2, O2, CO2) 4-gas (air, N2, O2, CO2)
Gas flow control2) TMFC R or TMFC
Exhaust analysis  

Optical density measurement  

Validation
Remote Device Monitoring and - -
Notifications (VisioNize®)
1)
Realized using multiple vessels 2) R = Rotameter, TMFC = Thermal Mass Flow Controller 3) OD measurement possible via third-party equipment
■ = standard, o = optional

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 15

SciVario® twin BioFlo® 120 BioFlo® 320

28 32 38
0.2 – 40 L 0.4 – 40 L 0.4 – 40 L
  

  

  

  

o  

o  

o  

2 Up to 8
Touchscreen Touchscreen Touchscreen
  

  

4-gas (air, N2, O2, CO2) 4-gas (air, N2, O2, CO2) 4-gas (air, N2, O2, CO2)
TMFC R or TMFC TMFC
  

3) 3) 3)

 - -

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
16

Our Portfolio – Your Choice

Model BioFlo® 720 Bioreactor BioFlo®/CelliGen 510 Fermentor/Bioreactor

Page 44 46
Working volume ranges1) 50 – 250 L 10.75 – 32 L
Single-use vessels 

Glass vessels, autoclavable

Stainless-steel vessels, SIP 

Interchangeable vessels 

Bacteria/yeasts/fungi  

Mammalian/animal cells  

Stem cells  

Insect cells  

Number of parallel units

Controller2) Touchscreen Touchscreen
Touchscreen controller  

BioCommand®  

DASware®
Gas mixing options 4-gas (air, N2, O2, CO2) 4-gas (air, N2, O2, CO2)
Gas flow control2) TMFC TMFC
Exhaust analysis  

Optical density measurement 3) 3)

Validation  
1)
Realized using multiple vessels 2) TMFC = Thermal Mass Flow Controller 3) OD measurement possible via third-party equipment

■ = standard

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 17

BioFlo® 610 Fermentor BioFlo® Pro Fermentor

48 50
16 – 100 L 45 – 2,400 L

 

 

 

Touchscreen Touchscreen
 

 

2-gas (air, O2) 2-gas (air, O2)

TMFC TMFC
 

3) 3)

 

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
18

Systems

DASbox® Mini Bioreactor System

Description Product features

The DASbox is a unique mini bioreactor system suitable for >> Parallel set-up of up to 24 bioreactors
microbial and cell culture as well as stem cell applications. It is >> Excellent scalability and reproducibility in both microbial and cell
designed as a 4-fold system with up to twenty-four parallel operating culture applications
bioreactors. With working volumes of 60 – 250 mL the DASbox is >> Supports industry standard glass bioreactors (DASbox Mini
the optimal tool for advanced process development and Design of Bioreactor) as well as BioBLU 0.3 Single-Use Bioreactors
Experiments (DoE) applications. All critical process parameters can >> Small working volumes save on the amount of cell material,
be precisely controlled. Liquid-free temperature control and exhaust medium and supplements required
condensation satisfy users with easy handling. >> Extremely compact system with a footprint of only 7.5 cm (3 in)
In addition to using industry standard glass bioreactors, the DASbox benchspace per vessel
can be equipped with Eppendorf BioBLU 0.3 Single-Use Bioreactors, >> Individual temperature control with liquid-free heating and cooling
all fully instrumented single-use mini bioreactors. (Peltier)
>> Liquid-free Peltier exhaust condenser with easy handling by
automatic slide in activation and slide out deactivation mode
Applications >> LC display with key process parameters and integrated alarm
>> Process development in cell culture and microbiology function simplifies monitoring
>> Controlled cultivation of stem cells >> Fully mass flow-controlled gas mixing with individual gas mixture
>> Design of Experiments (DoE) from air, N2, O2, and CO2, each directable either to headspace or
>> Media optimization sparger
>> Clone and cell line screening, strain characterization >> Standard sensors for precise measurement and control of
temperature, pH, DO, level and ORP (redox potential); optical pH
sensors available
>> Precise miniature variable speed pumps, continuous flow rates
down to 0.3 mL/h
>> Sealed magnetic overhead drives for single-use vessels and
direct overhead drives for autoclavable vessels; up- or downflow
selectable
>> Optional pull-out system for enhanced accessibility of bioreactors
and control unit

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 19

Systems

Parallel operation of up to 24 bioreactors makes the DASbox a The DASbox can be operated with BioBLU 0.3c, BioBLU 0.3sc, and
perfect fit for process development. 0.3f Single-Use Bioreactors for cell culture and microbial applications.

Ordering information
Description Order no.
DASbox® Mini Bioreactor System, for cell culture applications, max. 5 sL/h gassing
4-fold system 76DX04CC
8-fold system 76DX08CC
12-fold system 76DX12CC
16-fold system 76DX16CC
20-fold system 76DX20CC
24-fold system 76DX24CC
4-fold system for single-use vessels 76DX04CCSU
8-fold system for single-use vessels 76DX08CCSU
12-fold system for single-use vessels 76DX12CCSU
16-fold system for single-use vessels 76DX16CCSU
20-fold system for single-use vessels 76DX20CCSU
24-fold system for single-use vessels 76DX24CCSU
DASbox® Mini Bioreactor System, for microbial applications, max. 25 sL/h gassing
4-fold system 76DX04MB
8-fold system 76DX08MB
12-fold system 76DX12MB
16-fold system 76DX16MB
20-fold system 76DX20MB
24-fold system 76DX24MB
4-fold system for single-use vessels 76DX04MBSU
8-fold system for single-use vessels 76DX08MBSU
12-fold system for single-use vessels 76DX12MBSU
16-fold system for single-use vessels 76DX16MBSU
20-fold system for single-use vessels 76DX20MBSU
24-fold system for single-use vessels 76DX24MBSU

Suitable vessels for the DASbox can be found on pages 66 - 67.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
20

Systems

DASbox® Mini Bioreactor System

Technical specifications
Model DASbox® Cell Culture DASbox® Cell Culture DASbox® Microbiology DASbox® Microbiology
Single-Use Single-Use
Application Cell culture Cell culture Microbiology Microbiology
Number of parallel units Up to 24 Up to 24 Up to 24 Up to 24
Software DASware control, other DASware control, other DASware control, other DASware control, other
DASware optional DASware optional DASware optional DASware optional
User Interface Process computer with Process computer with Process computer with Process computer with
monitor monitor monitor monitor
Typical power consumption (4-fold 168 W (at 230 V)/ 168 W (at 230 V)/ 168 W (at 230 V)/ 168 W (at 230 V)/
system without process computer) 154 W (at 115 V) 154 W (at 115 V) 154 W (at 115 V) 154 W (at 115 V)
Dimensions (W × D × H, 4-fold 30 x 70 x 49 cm 30 x 70 x 49 cm 30 x 70 x 49 cm 30 x 70 x 49 cm
system without process computer) (12 x 28 x 19 in) (12 x 28 x 19 in) (12 x 28 x 19 in) (12 x 28 x 19 in)
Typical weight (4-fold system 44 kg 44 kg 44 kg 44 kg
without accessories)
Bioreactors
Vessels Glass vessels Single-use vessels Glass vessels Single-use vessels
Sterilization Autoclavable Pre-sterilized Autoclavable Pre-sterilized
Total volume 350 mL 380 mL 350 mL 380 mL
Agitation
Drive Direct overhead drives Magnetic overhead drives Direct overhead drives Magnetic overhead drives
Speed ranges 20 – 2500 rpm 20 – 500 rpm 20 – 2500 rpm 20 – 2000 rpm
Impellers Marine Pitched-blade Rushton-type Rushton-type
Gassing
Gas supply TMFC; overlay and/or TMFC; overlay and/or TMFC; sparger TMFC; sparger
sparger sparger
Standard gas flow rates 0.04 – 5 sL/h, 0.04 – 5 sL/h, 0.2 – 25 sL/h, 0.2 – 18 sL/h 0.2 – 25 sL/h,
0.04 – 3.5 sL/h CO2 0.04 – 3.5 sL/h CO2 CO2 0.2 – 18 sL/h CO2
Standard gas mixing Air, N2, O2 and/or CO2 Air, N2, O2 and/or CO2 Air, N2, O2 and/or CO2 Air, N2, O2 and/or CO2
Feeding
Feed lines per vessel 2 (standard)/ 2 (standard)/ 2 (standard)/ 2 (standard)/
4 (optional) 4 (optional) 4 (optional) 4 (optional)
Standard feed rates 0.3 – 9.5 mL/h to 0.3 – 9.5 mL/h to 0.3 – 9.5 mL/h to 0.3 – 9.5 mL/h to
(depending on tube diameter) 13 – 420 mL/h 13 – 420 mL/h 13 – 420 mL/h 13 – 420 mL/h
Monitoring and Control
Temperature control Liquid-free heating and Liquid-free heating and Liquid-free heating and Liquid-free heating and
cooling (Peltier) cooling (Peltier) cooling (Peltier) cooling (Peltier)
Standard temperature range 10 – 60°C 10 – 45°C 10 – 60°C 10 – 45°C
(at 25° C room temp.)
pH control CO2/base, and other CO2/base, and other Acid and/or base, and Acid and/or base, and
set-ups set-ups other set-ups other set-ups
DO control Cascade (O2 concentration, Cascade (O2 concentration, Cascade (agitation speed, Cascade (agitation speed,
gas flow rate), and other gas flow rate), and other O2 concentration, gas flow O2 concentration, gas flow
set-ups set-ups rate), and other set-ups rate), and other set-ups
ORP (redox) measurement - - Optional (select redox or Optional (select redox or
level) level)
Level/foam Optional Optional Optional (select redox or Optional (select redox or
level) level)
OD measurement Optional Optional Optional Optional
(DASGIP OD4) (DASGIP OD4) (DASGIP OD4) (DASGIP OD4)
Exhaust condensation Liquid-free (Peltier) Liquid-free (Peltier) Liquid-free (Peltier) Liquid-free (Peltier)
Exhaust analysis - - Optional Optional
(DASGIP GA4) (DASGIP GA4)

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 21

Systems

Together with the DASware software solutions, the DASbox supports Lab space is critical - The DASbox requires only 7.5 cm (3 in) of
process development following the Quality by Design (QbD) bench space per bioreactor.
approach.

Accessories
Description Order no.
DASbox® Overhead Drive, 20 – 2500 rpm, with cable L 1.6 m, direct-drive 78525185
DASbox® Overhead Drive, 20 – 2000 rpm, for BioBLU® 0.3, with cable L 1.6 m, magnetic-drive 78525186
DASbox® Autoclavable Carrier, for 4 vessels 76DXBKT4
DASbox® Autoclavable Carrier, stainless steel, labeled, for media bottles 78109113
DASbox® Feeding and Monitoring System MP8, without feed lines and addition bottles 76DXMP8
DASbox® Pull-Out System, for one DASbox® base unit 76DXRAIL
DASbox® GA4 Exhaust Analyzing Module, O2 0 – 100 %, CO2 0 – 25 %, including I/O option and accessories for 4 vessels 76DXGA4EX
DASbox® GA4 Exhaust Analyzing Module, O2 1 – 50 %, CO2 0 – 25 %, including I/O option and accessories for 4 vessels 76DXGA4X

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
22

Systems

DASGIP® Parallel Bioreactor Systems

Description Product features

DASGIP Parallel Bioreactor Systems for R&D and process >> Parallel operation of up to 16 glass or single-use bioreactors
development in both cell culture and microbiology allow for >> DASGIP Bioblock for advanced and user-friendly temperature
advanced bioprocess control and automation. Parallel processing, control
precise control of all relevant parameters, user-defined profiles, >> Suitable for cell culture and microbial fermentation
and innumerable automation features result in accelerated and >> Modular design of control units allows for flexible system
highly efficient process development. Our DASware software configurations that meet the demands of specific applications
solutions support DoE, process historians and comprehensive >> Control of agitation, pH, level, and DO (including customizable
data management. Configurable solutions address the unique cascade modes) in each bioreactor
requirements of microbial, mammalian and human cells, stem cell >> Variable speed pumps for accurate liquid addition and operation in
applications, as well as biofuel and biopolymer processes. batch, fed-batch, continuous, and cyclic perfusion mode
>> Optical absorbance measurement for online calculation of e.g.
OD600 or cell dry weight
>> TMFC individual gas mixing of air, N2, O2 and CO2
Applications >> Online calculation of OTR, CTR and RQ
>> Research and development in cell culture and microbiology >> DASware control software for advanced process control
>> Lab scale fermentation of aerobic and anaerobic bacteria, yeasts >> Compatible with DASware Software Suite for interconnectivity and
and fungi bioprocess information management
>> Cultivation of mammalian, insect and human cell lines
>> Special applications such as stem cell culture or biofuel/biopolymer
development

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 23

Systems

With DASware control 6, DASGIP Parallel Bioreactor Systems can be The DASGIP Bioblock can be used in a wide selection of vessels for
operated with up to 16 vessels. cell culture and microbiology, both glass and single-use.

Ordering information
Description Order no.
DASGIP® Parallel Bioreactor System, for cell culture, max. 50 sL/h gassing
4-fold system with DASGIP® Bioblock 76DG04CCBB
8-fold system with DASGIP® Bioblock 76DG08CCBB
12-fold system with DASGIP® Bioblock 76DG12CCBB
16-fold system with DASGIP® Bioblock 76DG16CCBB
4-fold system with DASGIP® Bioblock, for single-use vessels 76DG04CCSU
8-fold system with DASGIP® Bioblock, for single-use vessels 76DG08CCSU
12-fold system with DASGIP® Bioblock, for single-use vessels 76DG12CCSU
16-fold system with DASGIP® Bioblock, for single-use vessels 76DG16CCSU
DASGIP® Parallel Bioreactor System, for microbial applications, max. 250 sL/h gassing
4-fold system with DASGIP® Bioblock 76DG04MBBB
8-fold system with DASGIP® Bioblock 76DG08MBBB
12-fold system with DASGIP® Bioblock 76DG12MBBB
16-fold system with DASGIP® Bioblock 76DG16MBBB
4-fold system with DASGIP® Bioblock, for single-use vessels 76DG04MBSU
8-fold system with DASGIP® Bioblock, for single-use vessels 76DG08MBSU
12-fold system with DASGIP® Bioblock, for single-use vessels 76DG12MBSU
16-fold system with DASGIP® Bioblock, for single-use vessels 76DG16MBSU

i DASGIP Parallel Bioreactor Systems are configured to meet individual customer requirements. The systems shown are example configurations. Please contact us for more information.

Suitable vessels for the DASGIP Parallel Bioreactor Systems can be found on pages 68-71.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
24

Systems

DASGIP® Parallel Bioreactor Systems

Technical specifications
Model DASGIP® System for Cell Culture DASGIP® System for Microbiology
Application Cell culture Microbiology
Number of parallel units Up to 16 Up to 16
Software DASware control, other DASware optional DASware control, other DASware optional
User Interface Process computer with monitor Process computer with monitor
Typical power consumption (4-fold 480 W (at 230 V)/ 541 W (at 230 V)/
system without process computer) 432 W (at 115 V) 467 W (at 115 V)
(595 W/552 W without DASGIP Bioblock) (639 W/584 W without DASGIP Bioblock)
Dimensions (W × D × H, 4-fold 140 x 70 x 53 cm (55 x 28 x 21 in) 140 x 70 x 53 cm (55 x 28 x 21 in)
system without process computer)
Typical weight (4-fold system 78 kg 96 kg
without accessories) (60 kg without DASGIP Bioblock) (78 kg without DASGIP Bioblock)
Bioreactors
Vessels Glass/single-use vessels Glass/single-use vessels
Sterilization Autoclavable/ Autoclavable/
pre-sterilized pre-sterilized
Total volume 1.5 L to 2.3 L 1.3 L to 2.1 L
Agitation
Drive Direct/magnetic overhead drive Direct/magnetic overhead drive
Speed ranges 30 – 1,250 rpm (standard)/ 100 – 1,600 rpm (standard)/
100 – 1,600 rpm (optional) 30 – 1,250 rpm (optional)
Impellers Pitched-blade Rushton-type
Gassing
Gas supply TMFC; overlay and/or sparger TMFC/Rotameter; sparger
Standard gas flow rates 0.1 – 50 sL/h, 0.1 – 40 sL/h CO2 (DASGIP MX4/4, other 0.5 – 250 sL/h, 0.5 – 150 sL/h CO2 (DASGIP MX4/4H,
modules available) other modules available)
Standard gas mixing Air, N2, O2 and/or CO2 Air, N2, O2 and/or CO2
Feeding
Feed lines per vessel Up to 8 Up to 8
Standard feed rates 0.3 – 9.5 mL/h to 13 – 420 mL/h (DASGIP MP8) 0.3 – 9.5 mL/h to 13 – 420 mL/h (DASGIP MP8)
(depending on tube diameter) 10 – 70 mL/h to 0.4 – 5 L/h 10 – 70 mL/h to 0.4 – 5 L/h (DASGIP MP4)
(DASGIP MP4)
Monitoring and Control
Temperature control Heating and cooling integrated in DASGIP Bioblock Heating and cooling integrated in DASGIP Bioblock
(additional cooling fingers optional) (additional cooling fingers optional)
Standard temperature range 5 K above cooling agent temperature – 99°C1) (DASGIP 5 K above cooling agent temperature – 99°C1) (DASGIP
(at 25° C room temp.) Bioblock) Bioblock)
pH control Acid and/or base, and other set-ups
DO control Cascade (O2 concentration, gas flow rate), and other Cascade (agitation speed, optional O2 concentration,
set-ups optional gas flow rate), and other set-ups
ORP (redox) measurement - Optional
Level/foam Optional Optional
OD measurement Optional Optional
(DASGIP OD4) (DASGIP OD4)
Exhaust condensation Water-cooled or liquid-free (Peltier with DASGIP EGC4) Water-cooled or liquid-free (Peltier with DASGIP EGC4)
Exhaust analysis Optional Optional
(DASGIP GA4) (DASGIP GA4)
1)
Application-specific operating conditions apply.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 25

Systems

The DASGIP modules enable customized solutions for all With a wide variety of autoclavable and single-use vessels, DASGIP
requirements. For more information see pages 118 - 133. Parallel Bioreactor Systems can be adapted to a huge range of
applications.

Accessories
Description Order no.
Cable, for DASGIP® overhead drive RE30/RE40
L 1.3 m 78525121
L3m 78525112
Overhead Drive
RE30 (magnetic), 30 – 1250 rpm, for BioBLU® 1, for BioBLU® 1 78525189
RE30, 30 – 1250 rpm, for head plate port with M30 thread 78525187
RE40 (magnetic), 100 – 1600 rpm, for BioBLU® 1 78525198
RE40, 100 – 1600 rpm, for head plate port with M30 thread 78525188
Autoclavable Carrier, for 2 DASGIP® vessels 76DGBKT2

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
26

Systems

Bioprocess Autosampler

Description Product features

The Bioprocess Autosampler takes samples from multiple bioreactors >> Aseptic operation without the use of a laminar flow cabinet
and stores them tempered for later analysis. It is compatible with >> Compatible with differently-sized glass and BioBLU® Single-Use
glass and single-use bioreactors operated with a DASbox® Mini Bioreactors with working volumes of 60 mL to 1.8 L
Bioreactor System or a DASGIP® Parallel Bioreactor System. >> Suitable for 1.5 mL and 10 mL sample vials, up to 648 samples can
be stored
>> Enables regular sampling 24/7
Applications >> Sample storage between 4 and 40 °C.
>> Research and development in microbiology >> Sanitation procedure using ethanol is similar to procedures,
>> Small scale bioprocesses for aerobic and anaerobic bacteria and usually applied when sampling manually
yeasts >> Low dead volume minimizes the reduction of culture volume
>> Suitable for batch, fed-batch, and continuous processes >> Modular design that facilitates expansion of the number of
bioreactors to be sampled and retrofitting of existing bioprocess
systems
>> Control of the Bioprocess Autosampler is integrated in DASware®
control bioprocess software

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 27

Systems

Efficient sampling from bioreactors of Automating upstream bioprocess: operating 24/7, storing samples for later analysis. All using
different sizes. Different sample sizes with the same space as your bioreactor system!
aseptic operation and low dead volume.

Ordering information
Description Order no.
Bioprocess Autosampler, base unit, single head, with accessories
for 4 vessels, 1.6 m 7700 200 007
Bioprocess Autosampler, base unit, dual head, with accessories
for 8 vessels, 2.0 m 7700 200 008

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
28

Systems

SciVario® twin

Description Product features

The SciVario twin is a next-generation bioreactor control system >> Operation of 2 bioreactors (glass and/or BioBLU Single-Use
suitable for microbial and cell culture applications. The system is Bioreactors) in any combination of vessel types, independently run
capable of controlling two vessels at the same time, either glass or or in parallel
single-use. Compact, adaptable for changing requirements in the >> Suitable for cell culture and microbial fermentation
future, and its intuitive VisioNize® touch user interface make this >> Temperature control block for advanced and user-friendly
the ideal controller in R&D and process development. Digital sensor temperature control or temperature control via heat blankets and
technology, wide range pumps and gassing system, and a guided cooling fingers (benchtop bioreactors)
workflow to guide you step by step are just some of the features that >> Future proven bay-drawer concept for further extension and
will help you growing your cells. The controller integrates seamlessly reconfiguration
into the VisioNize Lab Suite, enabling remote monitoring and >> VisioNize touch: intuitive and exciting user interface supporting
notifications for your peace of mind. efficient and intelligent process control
>> Integrated digital sensor technology (Mettler Toledo ISM® and
Hamilton ARC®), supporting analog sensors for pH and DO and
Applications optical DO sensors
>> Research and development in cell culture and microbiology >> Optional PreSens® optical DO sensor technology for BioBLU
>> Lab scale fermentation of aerobic and anaerobic bacteria, yeasts Single-Use Bioreactors
and other or filamentous fungi >> Compact design, 16-40 cm of benchspace needed per vessel
>> Cultivation of mammalian and human cell lines >> Variable speed pumps for accurate liquid addition and operation in
>> Media optimization batch and fed-batch mode
>> Clone and cell line screening, strain characterization >> Wide range TMFC to allow for individual mixing of air, N2, O2, and
CO2 to headspace and/or submerged aeration
>> Compatible with DASware® control software for advanced process
control

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 29

Systems

With the compact design, you can make the most of precious bench space in the lab.

Ordering information
Description Order no.
SciVario® twin Fermenter/Bioreactor Control System, base unit
100 – 240 V/50/60 Hz, for 2 vessels 7600 100 001

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
30

Systems

SciVario® twin
Technical specifications
Model SciVario twin SciVario twin SciVario twin SciVario twin
Application Cell culture Cell culture Microbiology Microbiology
Software DASware control, DASware control, DASware control, DASware control,
VisioNize onboard VisioNize onboard VisioNize onboard VisioNize onboard
User Interface Touchscreen Touchscreen Touchscreen Touchscreen
Power supply 100 – 240 V, 50/60 Hz 100 – 240 V, 50/60 Hz 100 – 240 V, 50/60 Hz 100 – 240 V, 50/60 Hz
Dimensions (W × D × H) 30.6 × 34 × 75 cm / 12.0 × 30.6 × 34 × 75 cm / 12.0 × 30.6 × 34 × 75 cm / 12.0 × 30.6 × 34 × 75 cm / 12.0 ×
13.4 × 29.5 in 13.4 × 29.5 in 13.4 × 29.5 in 13.4 × 29.5 in
Weight w/o accessories 43.1 kg 43.1 kg 43.1 kg 43.1 kg
Bioreactors
Vessels Glass vessels Single-use vessels Glass vessels Single-use vessels
Sterilization Autoclavable Pre-sterilized Autoclavable Pre-sterilized
Total volume 1.5 - 4.3 L 1.8 L - 50 L 1.3 - 4.3 L 1.8 L - 5 L
Agitation
Drive Direct overhead drive Magnetic overhead drive Direct overhead drive Magnetic overhead drives
Speed ranges 25 - 1,250/ 25 - 1,250/ 25 - 1,250/ 25 - 1,250/
60 - 1,600 rpm 60 - 1,600 rpm 60 - 1,600 rpm 60 - 1,600 rpm
Impellers Pitched-blade Pitched-blade Rushton-type Rushton-type
Gassing
Gas supply TMFC; overlay and/or TMFC; overlay and/or TMFC; overlay and/or TMFC; overlay and/or
submerged submerged submerged submerged
Standard gas flow rates 0.1 - 1,200 sL/h 0.1 - 1,200 sL/h 0.1 - 1,200 sL/h 0.1 - 1,200 sL/h
(Air and O2) (Air and O2) (Air and O2) (Air and O2)
0.1 - 12 sL/h (N2 and CO2) 0.1 - 12 sL/h (N2 and CO2) 0.1 - 12 sL/h (N2 and CO2) 0.1 - 12 sL/h (N2 and CO2)
Standard gas mixing Air, N2, O2 and CO2 Air, N2, O2 and CO2 Air, N2, O2 and CO2 Air, N2, O2 and CO2
Feeding
Standard feed rates (depending on 4x small pump: 4x small pump: 4x small pump: 4x small pump:
tube diameter) 0.005 - 600 mL/h 0.005 - 600 mL/h 0.005 - 600 mL/h 0.005 - 600 mL/h
1x large pump: 1x large pump: 1x large pump: 1x large pump:
4.5 - 5,200 mL/h 4.5 - 5,200 mL/h 4.5 - 5,200 mL/h 4.5 - 5,200 mL/h
Monitoring and Control
Temperature control Heat blankets (optional Heat blankets (optional Heat blankets (optional Heat blankets (optional
cooling fingers) or cooling fingers) or cooling fingers) or cooling fingers) or
temperature control block temperature control block temperature control block temperature control block
with integrated heating with integrated heating with integrated heating with integrated heating
and cooling and cooling and cooling and cooling
Standard temperature range 10 – 70°C 25 – 40°C 10 – 70°C 25 – 40°C
(at 25° C room temp.)
pH control CO2 / base CO2 / base Acid and base Acid and base
DO control Cascade (O₂ concentration, Cascade (O₂ concentration, Cascade (agitation speed, Cascade (agitation speed,
gas flow rate) gas flow rate) O₂ concentration, gas flow O₂ concentration, gas flow
rate) rate)
Level/antifoam monitoring and yes yes yes yes
control
Exhaust condensation Liquid-free (Peltier) or Liquid-free (Peltier) or Liquid-free (Peltier) or Liquid-free (Peltier) or
water-based water-based water-based water-based

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Systems

Customizable and flexible thanks to the VisioNize® touch software on the 12 in touch display with ultra wide view angle,
innovative bay-drawer concept integrates the SciVario twin into the Eppendorf digital lab

Ordering information
Description Order no.
Drawer, for SciVario® twin
blank 7600 110 001
with 2 small peristaltic pumps 7600 110 002
with 1 big peristaltic pump 7600 110 003
optical pH 7600 110 004
Overhead Drive, for SciVario® twin
direct drive for glass vessel, MD30 7600 211 003
magnetic drive for single-use vessel, MD30 7600 211 004
magnetic drive for single-use vessels, MD30 7600 211 005
direct drive for glass vessel, MD40 7600 211 006
magnetic drive for single-use vessel, MD40 7600 211 007
magnetic drive for single-use vessels, MD40 7600 211 008
magnetic drive for single-use vessels, TB200 7600 211 010
Temperature Block, for SciVario® twin
for glass and single-use vessels, 0.7 – 1.8 L 7600 230 102
Heat blanket, for SciVario® twin
for glass and single-use vessels, 2.4 – 3.8 L 7600 230 201
for single-use vessels, 10 L 7600 230 202
for single-use vessels, 50 L 7600 230 203

Suitable vessels for the SciVario twin can be found on pages 68-75.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
32

Systems

BioFlo® 120

1
6

1. Easy-to-read 7 in integrated touchscreen

monitor

2. Three front-mounted pumps with

industry-standard easy load pump heads

5 3. Water recirculation module provides

precise temperature control and exhaust
condensing

4. High-precision thermal mass flow

controller (TMFC) or rotameter for gas
flow control with standard 4-gas mixing

5. Three user-defined analog input/output

4 connections on the advanced model

6. Connection for direct-drive and bi-

directional magnetic-drive motors

2
3

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 33

Systems

The BioFlo 120 Auto Culture modes allow 1-touch process control for Besides the BioBLU Single-Use Bioreactors for cell culture and
microbial and cell culture applications. microbial applications, water-jacketed or heat-blanketed autoclavable
vessels are available in four sizes.

Description Product features

The BioFlo 120 is a bench-scale bioreactor/fermentor system >> Scale-up from 250 mL to 40 L on a wide variety of autoclavable
perfectly suited for all levels of research and development. The and Eppendorf BioBLU Single-Use Bioreactors.
system was designed to be flexible to meet the wide-ranging needs >> Auto culture modes offer process control for microbial and cell
of scientists today. 24 interchangeable heat-blanketed and water- culture applications at the touch of a button.
jacketed autoclavable vessels are available, along with BioBLU >> Ready for process. Unbox and install in minutes.
Single-Use Bioreactors ranging from 250 mL to 40 L working volume. >> Save critical lab space with a minimal footprint.
Digital Mettler Toledo® ISM sensor technology is fully integrated >> Flexible universal connections for analog sensors or digital Mettler
allowing the user to choose between pH, redox, dissolved oxygen Toledo® ISM sensors offer unsurpassed flexibility.
(polarographic and/or optical), and carbon dioxide measurements >> User-defined DO cascades offer process flexibility.
without the need for additional equipment. A high-precision >> Automatic gas mixing algorithms for simplified control
expanded range thermal mass flow controller is available providing >> View your entire process with expanded trend screen.
the capability for low flow mammalian cell culture through high- >> Access your data from anywhere with Eppendorf SCADA, IP
demand microbial processes on a single controller. network, and remote monitoring capabilities.

Applications
>> Cultivation of mammalian, stem, insect, and plant cells
>> Bacterial, yeast, and fungal fermentation
>> Batch, fed-batch, continuous or perfusion

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
34

Systems

BioFlo® 120
BioFlo 120 Specifications
Control Station
Dimensions (W × D × H) 24.7 × 55.9 × 62.9 cm (9.7 × 22 × 24.8 in)
Net weight 14.8 kg (32.7 lb)
Touchscreen 7 in projected capacitive touchscreen
Communication 2 x USB (software updates, serial communication)
Utility Connection Requirement
Electrical IEC-C14 (with regional plug types) 100 – 120/208 – 240 (± 10 %) V, 50/60 Hz, 10 A, Single Phase
Water Quick-connect 10 psig (0.69 barg)
Gas supply (air, O2, N2, CO2) Push-connect fittings accept 1/4 in Autoclavable Single-use
tubing or hose barb fitting 10 psig (0.69 barg) 6 psig (0.44 barg)
Exhaust 0.5 psig (0.035 barg)
Operating conditions 10 – 30 °C, up to 80 % RH, non-condensing
Agitation
Direct drive 1 L: 25 – 1,500 rpm
3 L, 5 L, 10 L: 25 – 1,200 rpm
Magnetic drive (autoclavable 1 L, 3 L, or 5 L: 5 – 500 rpm
vessels) 10 L: 5 – 150 rpm
Magnetic drive (single-use vessels) BioBLU 1f & 3f: 5 – 1,200 rpm
BioBLU 1c: 5 – 500 rpm
BioBLU 3c, 5c, 5p, 10c & 14c: 5 – 200 rpm
BioBLU 50c: 5 – 150 rpm
Temperature
Autoclavable 1, 2, 5 L: 8 °C above coolant to 45 °C above ambient (0 °C – 70 °C absolute)1)
10 L: 8 °C above coolant to 40 °C above ambient (0 °C – 65 °C absolute)1)
BioBLU Single-Use Bioreactors BioBLU c bioreactors: 5 °C above ambient to 40 °C1)
BioBLU f bioreactors: 5 °C above coolant to 45 °C1)
Sensor type Pt100
Gas supply
Sparge 1 TMFC (0.04 – 20 SLPM, 0.01 - 5 SLPM or 0.002 - 1 SLPM) or 1 rotameter (multiple options available); ring or
microsparger
Sensors Communication Control range
pH Analog or digital Mettler Toledo ISM 2 – 12
DO Analog or digital Mettler Toledo ISM 0 – 200 % (air saturation)
Optical DO Digital Mettler Toledo ISM 0 – 200 % (air saturation)
Redox Analog or digital Mettler Toledo ISM (-)2,000 mV – (+)2,000 mV
CO2 Digital Mettler Toledo ISM 0 – 100 %
Pumps Pump head Fixed speed
Pumps 1, 2, & 3 Watson-Marlow 114DV 30 rpm (0 – 100 % duty cycle)
1)
Requires 115/230 V line voltage. Specifications cannot be guaranteed with operation at alternative line voltages
Specifications subject to change.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Systems

BioFlo 120 Advanced Control Station Bundles

Bundle includes Advanced Control Station with high precision TMFC, automatic four gas mix, 7 in (17.7 cm) integrated touchscreen, intuitive
bioprocess control software for control of both microbial fermentation and cell culture processes, three front mounted addition pumps, three
analog input/output connections, and region specific power cord.

Ordering information
Description Order no.
BioFlo® 120, advanced, plug type B
TMFC, 20 SLPM B120ACS000
TMFC, 1 SLPM B120ACS007
TMFC, 5 SLPM B120ACS014
BioFlo® 120, advanced, plug type CEE 7/7
TMFC, 20 SLPM B120ACS001
TMFC, 1 SLPM B120ACS008
TMFC, 5 SLPM B120ACS015
BioFlo® 120, advanced, plug type I
TMFC, 20 SLPM B120ACS002
TMFC, 1 SLPM B120ACS009
TMFC, 5 SLPM B120ACS016
BioFlo® 120, advanced, plug type J
TMFC, 20 SLPM B120ACS003
TMFC, 1 SLPM B120ACS010
TMFC, 5 SLPM B120ACS017
BioFlo® 120, advanced, plug type G
TMFC, 20 SLPM B120ACS004
TMFC, 1 SLPM B120ACS011
TMFC, 5 SLPM B120ACS018
BioFlo® 120, advanced, plug type N
TMFC, 20 SLPM B120ACS005
TMFC, 1 SLPM B120ACS012
TMFC, 5 SLPM B120ACS019
BioFlo® 120, advanced, plug type D
TMFC, 20 SLPM B120ACS006
TMFC, 1 SLPM B120ACS013
TMFC, 5 SLPM B120ACS020

Suitable vessels for the BioFlo 120 can be found on pages 88-95.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
36

Systems

BioFlo® 120
BioFlo 120 Standard Control Station Bundles
Bundle includes Standard Control Station manual flow control, automatic four gas mix, 7 in (17.7 cm) integrated touchscreen, intuitive
bioprocess control software for control of both microbial fermentation and cell culture processes, three front mounted addition pumps, and
region specific power cord.

Ordering information
Description Order no.
BioFlo® 120, standard, plug type B
Rotameter, 0.5 SLPM B120SCS000
Rotameter, 1 SLPM B120SCS010
Rotameter, 2.5 SLPM B120SCS020
Rotameter, 5 SLPM B120SCS030
Rotameter, 10 SLPM B120SCS040
Rotameter, 25 SLPM B120SCS050
BioFlo® 120, standard, plug type CEE 7/7
Rotameter, 0.5 SLPM B120SCS001
Rotameter, 1 SLPM B120SCS011
Rotameter, 2.5 SLPM B120SCS021
Rotameter, 5 SLPM B120SCS031
Rotameter, 10 SLPM B120SCS041
Rotameter, 25 SLPM B120SCS051
BioFlo® 120, standard, plug type I
Rotameter, 0.5 SLPM B120SCS002
Rotameter, 1 SLPM B120SCS012
Rotameter, 2.5 SLPM B120SCS022
Rotameter, 5 SLPM B120SCS032
Rotameter, 10 SLPM B120SCS042
Rotameter, 25 SLPM B120SCS052
BioFlo® 120, standard, plug type J
Rotameter, 0.5 SLPM B120SCS003
Rotameter, 1 SLPM B120SCS013
Rotameter, 2.5 SLPM B120SCS023
Rotameter, 5 SLPM B120SCS033
Rotameter, 10 SLPM B120SCS043
Rotameter, 25 SLPM B120SCS053
BioFlo® 120, standard, plug type G
Rotameter, 0.5 SLPM B120SCS004
Rotameter, 1 SLPM B120SCS014
Rotameter, 2.5 SLPM B120SCS024
Rotameter, 5 SLPM B120SCS034
Rotameter, 10 SLPM B120SCS044
Rotameter, 25 SLPM B120SCS054
BioFlo® 120, standard, plug type N
Rotameter, 0.5 SLPM B120SCS005
Rotameter, 1 SLPM B120SCS015
Rotameter, 2.5 SLPM B120SCS025
Rotameter, 5 SLPM B120SCS035
Rotameter, 10 SLPM B120SCS045
Rotameter, 25 SLPM B120SCS055
BioFlo® 120, standard, plug type D
Rotameter, 0.5 SLPM B120SCS006
Rotameter, 1 SLPM B120SCS016
Rotameter, 2.5 SLPM B120SCS026
Rotameter, 5 SLPM B120SCS036
Rotameter, 10 SLPM B120SCS046
Rotameter, 25 SLPM B120SCS056

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Systems

BioFlo/CelliGen 115 and BioFlo 120 replacement parts

Description BioFlo/CelliGen 115 BioFlo 120
Order no. Order no.
Cables
pH sensor, analog P0720-2276 1390810400
DO sensor, analog P0720-2336 1390810600
Redox sensor, analog P0720-27631) 1390810400
Sensor cable, ISM - M1379-8108
Optical DO sensor, ISM - M1379-8107
Foam sensor cable M1369-8035 (2 connections) 1390811000 (1 connection)/
1390811100 (3 connections)
Temperature sensor (RTD) M1369-8019 All vessels except BioBLU 1: 1390810000,
BioBLU 1: M1379-8112
Agitation motors
Autoclavable fermentation vessels (1 L/2 L) M1369-3120 1390080000
Autoclavable fermentation vessels (5 L/10 L) M1369-3125 1390080000
Autoclavable cell culture vessels, direct drive M1369-3135 1390080000
Autoclavable cell culture vessels, magnetic drive M1369-3130 M1379-9931
BioBLU 1c/1f - M1379-0850
BioBLU 3c/5c/5p/10c/14c/50c M1369-3130 M1379-9931
BioBLU 3f - 1386080000
Heat blankets
Autoclavable vessel 1 L M1369-8021 1390890000
Autoclavable vessel 2 L M1369-8022 1390890100
Autoclavable vessel 5 L M1369-8020 1390890200
Autoclavable vessel 10 L M1369-8023 1390890300
BioBLU 1c/1f - M1379-8200
BioBLU 3c/5c/5p/3f M1379-8116 M1379-8116
BioBLU 14c/10c M1379-8114 M1379-8114
BioBLU 50c M1379-8117 M1379-8117
BioBLU exhaust heater M1379-8115 M1379-8115
Heat blanket adaptor for BioBLU M1386-8125 1386811900
Base heaters for autoclavable vessels
1 L/2 L M1369-3107 1390310700
5 L/10 L M1369-3108 1390310800
1)
For BioFlo/CelliGen 115, a redox transmitter is required. See page 166 for details

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
38

Systems

BioFlo® 320

1.Control up to eight systems 2.Compact design with left- and 3. Universal control for both 4. Interchangeable autoclavable
from a single user interface right-handed orientation for microbial and cell culture and BioBLU® Single-Use
industry-leading minimum applications Bioreactors
footprint

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 39

Systems

A compact design, available in left- and right-handed orientation Easy-load pump heads provide quick installation of the pump tubing.
makes the BioFlo 320 the smarter solution for your laboratory.

Description Product features

The BioFlo 320 seamlessly blends beauty and utility in one all- >> Interchangeable autoclavable and BioBLU Single-Use Bioreactors
inclusive package. An industrial design, flexibility between >> Integrated Mettler Toledo Intelligent Sensor Management (ISM)
interchangeable autoclavable and single-use bioreactors, intelligent platform
sensors, Ethernet connectivity, and a software package are only a few >> Control up to eight systems from a single user interface
of the features. Extensive options give you the customizable solution >> Universal control for both microbial and cell culture applications
your lab requires. Combined with the strict quality standards that all >> Field-upgradable TMFC drawers for sparge and overlay gas
Eppendorf products are held to, the BioFlo 320 truly is the premium >> Enhanced software package with new cascade and time profile
choice in benchtop bioprocess systems. features
>> Built-in optical pH sensing technology for use with the BioBLU
Single-Use Bioreactors
Applications >> DeltaV™ compatibility available through DeltaV Discovery and
>> Research and development in cell culture and microbiology DeltaV ProPlus PAS
>> Bench- and pilot-scale fermentation of aerobic and anaerobic >> Up to six integrated pumps capable of operating in variable-speed
bacteria, yeasts, and fungi mode
>> Cultivation of mammalian, insect, and human cell lines >> Eight independently controlled process gas supplies
>> Specialized applications such as stem cell culture or biofuel/ >> Validation packages available for GMP regulated processes
biopolymer development >> Compatible with DASware Software Suite or BioCommand for
>> Specialized packed-bed impeller for vaccine production in bioprocess information management
anchorage and non-anchorage dependent cell lines
>> Suitable for batch, fed-batch, and continuous/perfusion processes
>> Validation packages available for GMP-regulated processes

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
40

Systems

BioFlo® 320
Control station
Dimensions (W × D × H) 40.6 × 40.6 × 66.0 cm (16 × 16 × 26 in)
Net weight 32 kg (70 lb)
Touchscreen 15 in Projected Capacitive Touchscreen
Communication 2 × USB (software updates, serial communication)

Ethernet (SCADA, IP Network)

3 × analog input/output (4 - 20 mA/0 - 5 V/0 - 10 V)
Agitation
Direct drive 25 - 1,500 rpm (1 L and 3 L)/ 25 - 1,200 rpm (5L and 10 L)
Magnetic drive (autoclavable 10 - 500 rpm (1 L, 3 L, and 5 L)/10 – 150 rpm (10 L)
vessels)
Magnetic drive BioBLU 1c: 10 – 500 rpm, BioBLU 1f: 10 – 1,200 rpm, BioBLU 3f: 25 – 1,200 rpm, BioBLU 3c/5c/5p and BioBLU
10c/14c: 10 – 200 rpm, BioBLU 50c: 10 – 150 rpm
Temperature
Water jacketed 5 °C above coolant to 55 °C above ambient (80 °C max)
Stainless-steel dish bottom 5 °C above coolant to 65 °C above ambient (90 °C max; 85 °C max for 10 L)
Single-use 5 °C above ambient to 40 °C (cell culture)/45 °C (microbiology)
Sensor type Pt100
Gassing
Sparge 1, 3, or 4 TMFC
Overlay/headspace 1 TMFC
Sensors Communication Control Range
pH Analog or Digital Mettler Toledo® ISM 2 - 12 pH
Optical pH Digital (Presens) 6 - 8 pH
DO Analog or Digital Mettler Toledo ISM 0 - 200 % (air saturation)
Optical DO Digital Mettler Toledo ISM 0 - 200 % (air saturation)
Redox Analog or Digital Mettler Toledo ISM (-)2,000mV - (+)2,000mV
CO2 Digital Mettler Toledo ISM 0 - 100 %
Pumps Pump Head Variable Speed Fixed Speed
Pumps 1, 2, and 3 Watson Marlow® 114DV 5 - 25 rpm 25 rpm (0-100 % duty cycle)
Pump 4 Watson Marlow 314D 20 - 100 rpm 100 rpm (0-100 % duty cycle)
External pumps 1 and 2 Watson Marlow 120U/DV 0.1-200 rpm N/A
Utility Connection Requirement
Electrical IEC (with regional plug type) 100-120/208-240 VAC, 50/60Hz, 20 A, single phase
Water Stainless-steel quick-connect 10 psig (0.69 barg)
Gas Supply (Air, O2, N2, CO2) Push-connect Autoclavable Single-use
10 psig (0.69 barg) 6 psig (0.44 barg)
Exhaust 0.5 psig (0.035 barg)
Operating conditions 10 - 30 °C, up to 80 % RH, non-condensing

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Systems

The BioFlo 320 is compatible with 250 mL - 40 L BioBLU Single-Use Control up to eight units from a single user interface.
Bioreactors.

Ordering information
Description Order no.
Validation, for BioFlo® 320, control station validation M1379-0102
BioFlo® 320, base control station, with water connection 1379 963 011
BioFlo® 320, base control station, no water connection 1379 962 911
BioFlo® 320, left-handed orientation/four front-mounted peristaltic pumps (3 @ 5 – 25 rpm/1 @ 20 – 100 rpm) 1379 963 211
BioFlo® 320, right-handed orientation/four front-mounted peristaltic pumps (3 @ 5 – 25 rpm/1 @ 20 – 100 rpm) 1379 963 411
BioFlo® 320
sparge gas option, 1 TMFC (0.002 – 1.0 SLPM) 1379 501 011
sparge gas option, 1 TMFC (0.04 – 20 SLPM) 1379 501 111
sparge gas option, 3 TMFC (0.002 – 1.0 SLPM) 1379 501 211
sparge gas option, 3 TMFC (0.04 – 20 SLPM) 1379 501 311
sparge gas option, 4 TMFC (0.002 – 1.0 SLPM) 1379 501 411
sparge gas option, 4 TMFC (0.04 – 20 SLPM) 1379 501 511
BioFlo® 320, overlay gas option, 1 TMFC (0.05 – 5 SLPM) 1379 502 111
BioFlo® 320, no overlay gas option module 1379 963 511
BioFlo® 320, secondary sensor module 1379 963 611
BioFlo® 320, no secondary sensor module 1379 963 711
BioFlo® 320, optical pH module 1379 963 811
BioFlo® 320, no optical pH module 1379 963 911
BioFlo® 320, equipment connection module 1379 964 011
BioFlo® 320, no equipment connection module 1379 964 111

Suitable vessels for the BioFlo 320 can be found on pages 96 – 103.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
42

Systems

BioFlo® 320

Ordering information
Description Order no.
Scale, vessel volume, 150 kg M1379-8188
Tubing, for BioFlo®/CelliGen 310 and BioFlo® 320, all vessel sizes M1287-9911
Pivot Arm Mount, for BioFlo® 320 touchscreen monitor M1379-9905
Touchscreen Monitor Bundle, includes desk mount and 1 m cable set, for BioFlo® 320 M1379-9906
Sparge Gas Drawer, field-installed
1 TMFC (0.002 – 1.0 SLPM) M1379-5010
1 TMFC (0.04 – 20 SLPM) M1379-5011
3 TMFC (0.002 – 1.0 SLPM) M1379-5012
3 TMFC (0.04 – 20 SLPM) M1379-5013
4 TMFC (0.002 – 1.0 SLPM) M1379-5014
4 TMFC (0.04 – 20 SLPM) M1379-5015
Overlay Gas Option Drawer, field-installed
1 TMFC (0.05 – 5 SLPM) M1379-5021
Secondary Sensor Module, field-installed M1379-9636
Optical pH Module, field-installed M1379-9638
Equipment Connection Module, field-installed M1379-9640

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Systems

Universal connections for four analog or digital Mettler Toledo ISM With four pumps of two different sizes, the BioFlo 320 is suitable for
sensors reduce sensor complexity. a large range of bioreactor sizes.

BioFlo/CelliGen 310 and BioFlo 320 replacement parts

Description BioFlo/CelliGen 310 BioFlo 320
Order no. Order no.
Cables
pH sensor, analog P0720-2273 M1379-8104
DO sensor, analog P0720-2333 M1379-8106
Redox sensor, analog P0720-22751) M1379-8105
Sensor cable, ISM - M1379-8108
Optical DO sensor, ISM - M1379-8107
Foam sensor cable M1297-8032 M1379-8109
Temperature sensor (RTD) M1294-8013 All vessels except BioBLU 1: M1379-8100,
BioBLU 1: M1379-8112
Agitation motors
Autoclavable fermentation vessels, M1287-0800 M1379-0800
direct drive
Autoclavable cell culture vessels, M1287-0750 M1379-0750
magnetic drive
BioBLU® 1c/1f - M1379-0850
BioBLU® 3c/5c/5p/10c/14c/50c - M1379-9931
BioBLU® 3f - 1386080000
Heat blankets
BioBLU® 1c/1f - M1379-8200
BioBLU® 3c/5c/5p M1379-8116 M1379-8116
BioBLU® 14c/10f/10c M1379-8114 M1379-8114
BioBLU® 50c M1379-8117 M1379-8117
BioBLU® Exhaust Heat Blanket M1379-8115 M1379-8115
1)
For BioFlo/CelliGen 310, the secondary pH/redox/DO option is required. See page 166 for details

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
44

Systems

BioFlo® 720

Description Product features

The BioFlo® 720 bioreactor control system was designed to save >> Compatible with Thermo Scientific™ HyPerforma™ 5:1 bioreactors
time and to mitigate risks. The well-known BioFlo software has been from 50 L to 2,000 L
enriched with a variety of new features to automate your workflow. >> High performance Mass Flow Controllers capable of up to 500:1
To ensure maximum flexibility and scalability, the new BioFlo 720 turn-down allowing multiple vessel sizes to be run from the same
bioreactor controller is compatible with the Thermo Scientific™ controller
HyPerforma™ 5:1 Single-Use Bioreactors (SUBs). The combined >> Suitable for analog & digital sensors (Mettler-Toledo® ISM and
solutions offer paramount scalability from 50 L to 2000 L with single- Hamilton® ARC)
use technology. >> Available with dual sparge, overlay and CO2 stripping options
>> Field-upgradeable options for future expansion of the system
>> Documentation packages available to help qualifying the system
for use in GMP environments1)
Applications >> Intuitive software tools such as Auto Calibrate and Auto Inflate
>> Tech transfer from R&D to production reduce preparation time, maximizing system efficiency
>> Pilot and production-scale bioreactor system for mammalian, >> Integrated Scale Up Assist software simplifies the workflow and
insect, and human cell lines calculations necessary to scale up and scale down
>> Suitable for batch, fed-batch, and continuous/perfusion processes >> Flexible choices for SUBs and Bioprocess Containers (BPCs)
>> Validation packages available for GMP-regulated processes1) >> Mobile enclosure with a small footprint (0.7 m2/7.55 sq ft) fitting
through a standard lab door
>> Watson-Marlow peristaltic pumps for flow rates in a range of
1.2 mL/min - 3.3 L/min
1)
BioFlo 720 is not a medical device as defined by the Food and Drug Administration or other regulatory
authorities.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 45

Systems

Easy to reach sensor location suitable for digital ISM and ARC Easy-load pump heads for fast and convenient tubing installation.
sensors.

Ordering information
Description Order no.
BioFlo® 720, bioreactor/fermenter control system
200 – 240 V/50/60 Hz, with three integrated Watson-Marlow® 314 peristaltic pumps 1385 200 011
200 – 240 V/50/60 Hz, with three integrated Watson-Marlow® 314 peristaltic pumps and two integrated Watson-Marlow® 520 peristaltic 1385 200 111
pumps

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
46

Systems

BioFlo®/CelliGen 510

Description Applications
The BioFlo 510 Fermentors and CelliGen 510 Bioreactors are >> Laboratory- and pilot-scale fermentation of aerobic and anaerobic
intermediate systems ideal for pilot through production applications. bacteria, yeasts, and fungi
These compact systems fit on a benchtop or on an optional mobile >> Laboratory- and pilot-scale cell culture of mammalian, insect, and
table. A modular design and wide variety of standard and optional human cell lines
components provide the flexibility to customize these systems to >> Special applications such as stem cell culture, vaccine, monoclonal
meet a variety of process requirements. antibody or biofuel/biopolymer production
>> Suitable for batch, fed-batch, and continuous/perfusion processes

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 47

Systems

Working volume
>> 10.75 L to 32.0 L

Gas flow
>> Various combinations of one, two, three or four TMFCs:
>> 0.06 – 3 SLPM
>> 0.3 – 15 SLPM
>> 0.6 – 32 SLPM
>> 1 – 64 SLPM
>> Air Wash System with TMFC (0 – 15 or 0 – 32 SLPM)
>> Overlay with TMFC (0 – 15 or 0 – 32 SLPM)
>> Overlay (valve only)

>> Exhaust line

>> Exhaust condenser
>> Manual or automatic pressure control

Impellers
>> Rushton-type – Used commonly for robust cell lines such as
bacteria, yeasts, and algae, where maximum OTR is desired.
Provided as standard on BioFlo 510 fermentors
>> Pitched-blade – Commonly used with mammalian, insect or
other shear sensitive cell lines for batch, fed-batch or continuous Numerous ports in the vessel head plate and sidewall provide
cultures. Produces axial and radial mixing. Provided as standard flexibility to position sensors, spray balls, addition valves, pressure
with the CelliGen 510 bioreactors. transducers, and more.
>> Marine – Commonly used with mammalian, insect, or other shear
sensitive cell lines for batch, fed-batch or continuous cultures.
Produces axial mixing Validation
>> Spinfilter – For suspension or microcarrier cultures where a dip >> Basic Package
tube inside the filter withdraws cell media as harvest or waste >> Basic Plus Package
>> Cell-Lift – Specially designed to provide uniform circulation for >> Enhanced Package
both suspension and microcarrier cultures. Can be used with
optional decanting columns for perfusion cultures Utility regulator & pre-filter kits
>> Packed-bed – For secreted products from anchorage-dependent >> Process air pre-filter/regulator kit
and suspension cells, this impeller immobilizes cells in a bed of >> Instrument air pre-filter/regulator kit
Fibra-Cel® Disks to provide extremely high cell densities >> Water pre-filter/regulator kit
>> Process steam regulator kit
Sensors >> Utility steam pre-filter/regulator kit
>> Single or redundant pH/DO >> Utility connection kit
>> Redox
>> Foam/level Additional options
>> Sprayballs for clean-in-place of vessel
Addition and sampling >> External variable-speed pumps
>> Resterilizable sampling valve >> External scales
>> Resterilizable addition valves (4 max) >> Spare parts kits
>> Resterilizable addition/harvest valve with dip tube (2 max) >> Preventive maintenance kits
>> 1.5 in sanitary fitting 7-port septum >> Mobile table
>> Addition vessels (glass/stainless steel) >> System passivation
>> Decanters >> Glycol/chiller heat exchanger
>> Sterile sampling assembly

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
48

Systems

BioFlo® 610

Description Applications
The BioFlo 610 is a compact, mobile, pilot-plant fermentor for >> Pilot-scale fermentation of aerobic and anaerobic bacteria, yeasts,
process development and small-scale production. A modular and fungi
design and wide variety of standard and optional components >> Special applications such as biofuel or biopolymer production
provide the flexibility to customize the system to meet your process >> Suitable for batch, fed-batch, and continuous processes
requirements.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 49

Systems

Working volumes Validation

>> 16.0 L to 50.0 L >> Basic Package
>> 32.0 L to 100.0 L >> Basic Plus Package
>> Enhanced Package
Gas flow control
>> Single gas Utility regulator and pre-filter kits
>> Two gas >> Process air pre-filter/regulator kit
>> Overlay (valve only) >> Instrument air pre-filter/regulator kit
>> Water pre-filter/regulator kit
Gas flow >> Process steam regulator kit
>> 1 TMFC, 50 L vessels, 1.5 – 75 SLPM >> Utility steam pre-filter/regulator kit
>> 1 TMFC, 100 L vessels, 3.0 – 150 SLPM >> Utility connection kit
>> 2 TMFC, 50 L vessels, 1.5 – 75/0.6 - 32 SLPM
>> 2 TMFC, 100 L vessels, 3.0 – 150/1.0 - 64 SLPM Additional options
>> Sprayballs for clean-in-place of vessel
Exhaust line >> External variable-speed pumps
>> Exhaust condenser >> External scales
>> Automatic pressure control >> Spare parts kits
>> Preventive maintenance kits
Impellers >> System passivation
>> Rushton-type, standard >> Low pressure seal alarm for double mechanical seal
>> Pitched-blade, optional >> Glycol/chiller heat exchanger
>> Marine, optional

Sensors
>> Single or redundant pH/DO
>> Redox
>> Foam/level

Addition and sampling

>> Resterilizable sampling valve
>> Resterilizable addition valves (4 max)
>> 1.5 in sanitary fitting 7-port septum
>> Addition vessels (glass/stainless steel)
>> Sterile sampling assembly

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
50

Systems

BioFlo® Pro

Description Applications
BioFlo Pro fermentors are modular systems designed for quick >> Pilot- and large-scale fermentation of aerobic and anaerobic
delivery, dependable operation, and system flexibility, all at an bacteria, yeasts, fungi, and insect cells
affordable price. BioFlo Pro systems utilize industry-standard >> Special applications such as biofuel/biopolymer production
components for dependable operation, and an Allen Bradley® >> Suitable for batch, fed-batch, and continuous processes
CompactLogix™ programmable-logic controller (PLC) for easy
integration into any production facility. The modular design enables
multiple options to be added, removed, or changed at any time to
meet your various process requirements. Vessels available in 120,
240, 400, 1,200, and 2,400 liter sizes.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Systems

Working volumes
>> 45 L to 120 L
>> 68 L to 240 L
>> 103 L to 400 L
>> 375 L to 1,200 L
>> 750 L to 2,400 L

Impellers
>> Rushton-type
>> Pitched-blade
>> Marine

Air inlet line

>> Thermal Mass Flow Controller
>> Dual inlet air filters (in series)
>> Dual-inlet air flters with test integrity ports
>> Gas overlay
>> 2-gas mix (O2 supplementation)

Exhaust line
>> Exhaust condenser
>> Manual or automatic backpressure control
>> Dual exhaust filters (in parallel)
>> Single or dual exhaust filters with test integrity ports

Pumps
>> Four built-in fixed-speed addition pumps
>> External variable-speed pumps

Housings/sensors
>> pH/DO analog and digital ISM sensors and transmitters
>> Redox sensor and transmitter
>> Retractable sensor housings BioFlo Pro fermentors offer working volumes of up to 2,400 L.
>> Redundant sensor kits

Foam kits
>> Foam kit Additional otions
>> High-foam kit >> Clean-in-place option (transfer panel/spray balls)
>> High-high foam kit >> Glycol/chiller heat exchanger
>> Low pressure seal alarm for double-mechanical seal
Utility regulator and pre-filter kits >> Vessel electropolish
>> Instrument air pre-filter/regulator kit >> System passivation
>> Water pre-filter/regulator kit >> Transfer lines (resterilizable)
>> Process steam regulator kit >> Addition vessel scales
>> Utility steam pre-filter/regulator kit >> DeltaV™ connectivity
>> Utility connection kit
Validation packages
Addition and sampling >> Basic Package
>> Sampling valve (Resterilizable) >> Basic Plus Package
>> Sterile sampling assembly >> Enhanced Package
>> Resterilizable addition valves
Parts kits
Vessel volume/weight >> Spare parts kits
>> Vessel volume via differential pressure >> Preventive maintenance kits
>> Load cells
>> Level sensor

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
52

Bioreactors

Dependability through proven design

With renowned polymer expertise, Eppendorf is proud to offer the largest portfolio of rigid-walled stirred-tank

single-use vessels – in small, bench and pilot scale. A wide range of industry standard glass bioreactors for the

cultivation of microbial, mammalian and human cells as well as phototrophic organisms completes our small and

bench scale vessel offering.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 53

Bioreactors

>> BioBLU® c Single-Use Bioreactors 58 - 61

>> BioBLU® f Single-Use Bioreactors 62 - 65
>> BioBLU® Single-Use Bioreactors Adaptor Kits 66 - 67
>> DASbox® Mini Bioreactor 68 - 69
>> DASGIP® Bioblock / SciVario® twin Spinner Vessels 70 - 71
>> DASGIP® Bioblock / SciVario® twin Stirrer Vessels 72 - 73
>> SciVario® twin Benchtop Bioreactors for Cell Culture 74 - 75
>> SciVario® twin Benchtop Bioreactors for Microbiology 76 - 77
>> DASbox®, DASGIP®,
and SciVario® twin Bioreactor Accessories 78 - 89
>> BioFlo® 120 Bioreactors 90 - 95
>> BioFlo® 120 Bioreactors Accessories 96 - 97
>> BioFlo® 320 Bioreactors 98 - 103
>> BioFlo® 320 Bioreactors Accessories 104 - 105
>> BioFlo®/CelliGen Bioreactors Replacement Parts 106 - 107

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
54

Our Portfolio – Your Choice

Model BioBLU® Single-Use Bioreactors DASbox® Mini Bioreactor

Page 58 68
Working volumes 100 - 250 mL, 60 - 250 mL
cell culture 320 mL - 1.25 L,
1.25 - 3.75 L,
3.5 - 10 L,
18.0 - 40.0 L
Working volumes 65 – 250 mL, 60 - 250 mL
microbiology 250 mL – 1.25 L,
1.25 – 3.75 L
Standard set-up DASbox (BioBLU 0.3) DASbox
DASGIP Bioblock (BioBLU 1)
SciVario twin (BioBLU 1, 3, 10, and 50)
BioFlo 120/320 (BioBLU 1, 3, 5, 10, 14, and 50)
Autoclavable 

Single-use 

Packed-bed impeller 

Cell-lift impeller
Bacteria/yeasts/fungi  

Plant cells/algae 

Mammalian/animal cells  

Insect cells  

Magnetic overhead drive 

Direct overhead drive 

Exhaust treatment Peltier-cooled/electric heat band/water-cooled Peltier-cooled

■ = standard, o = optional

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 55

DASGIP® Bioblock and SciVario twin SciVario twin Bioreactors BioFlo® 120 Bioreactors
Bioreactors
70 74 90
250 – 700 mL, 700 mL – 2.7 L, 0.4 - 10.5 L
350 mL – 1.0 L, 800 mL – 3.8 L
350 mL – 1.5 L

200 mL – 1.0 L, 700 mL – 2.7 L, 0.4 - 10.5 L

400 mL – 1.5 L, 800 mL – 3.8 L
400 mL – 1.8 L
DASGIP Bioblock / SciVario twin SciVario twin BioFlo 120

  

  

  

  

  

  

Water-cooled Water-cooled Water-cooled

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
56

Our Portfolio – Your Choice

Model BioFlo® 320 Bioreactors BioFlo®/CelliGen 510

Page 98
Working volumes 0.6 - 10.5 L 10.75 - 32 L
cell culture
Working volumes 0.6 - 10.5 L 10.75 - 32 L
microbiology
Standard set-up BioFlo 320 Benchtop/mobile table
Autoclavable 

Single-use
Sterilize-in-place 

Packed-bed impeller o o
Cell-lift impeller o o
Bacteria/yeasts/fungi  

Plant cells/algae  

Mammalian/animal cells  

Insect cells  

Magnetic overhead drive  

Direct overhead drive  

Exhaust treatment Water-cooled Water-cooled/

electric heat band
■ = standard, o = optional

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 57

BioFlo® 610 BioFlo® Pro

- -

16 - 100 L 45 - 2,400 L

Mobile skid Skid-mounted

 

 

 

 

Bottom drive Bottom drive

Water-cooled/ Water-cooled/
electric heat band electric heat band

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
58

Bioreactors

BioBLU® c Single-Use Bioreactors

Description Product features

Eppendorf BioBLU Single-Use Bioreactors combine all the >> Single-use, stirred-tank, rigid-walled bioreactors available in sizes
advantages of single-use technology with the trusted performance ranging from 100 mL to 40 L working volume
and scalability of a stirred-tank design. Single-use bioreactors >> All BioBLU c bioreactor sizes available with pitched-blade
eliminate autoclaving, improve turn-around time, and reduce overall impellers for cell culture applications
costs. The BioBLU portfolio has grown to accommodate users from >> BioBLU 0.3sc bioreactors are designed for stem cell applications in
early research and development through process development. research and development
BioBLU c and p bioreactors cover a wide variety of processes, >> BioBLU 5p bioreactors with Eppendorf-exclusive packed-bed
including shear-sensitive suspension cell cultures and adherent cell impeller, pre-loaded with Fibra-Cel® Disks
line development. >> Unique non-invasive pH and DO sensor technology drastically
reduces contamination risks (industry standard autoclavable pH
sensors available for pH measurement)
Applications >> Sealed magnetic drive with fully enclosed bearings maintain vessel
>> Insect, mammalian, and human suspension cell lines sterility
>> Stem cells >> Bioreactors are assembled with sparger, overlay, gas filters for
>> Adherent cells inlet and exhaust as well as penetrations for pH, DO, temperature,
liquid additions, sampling, and harvest
>> For use with Eppendorf benchtop bioreactor systems
>> Adaptor kits for legacy bioreactor controllers available

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Bioreactors

The BioBLU 0.3sc Single-Use Bioreactor is equipped with an 8-blade BioBLU 5p Single-Use Bioreactors create optimum growth conditions
impeller ensuring gentle mixing and reduced cell settling for stem for adherent cells and perfusion culture.
cell process development.

Ordering information
Order no. Order no.
Description (β-irradiated) (X-ray irradiated)
Vessel Working Volume Sparger Impeller pH Quantity
BioBLU® 0.3c 100 mL - 250 mL Open pipe 1x pitched-blade Standard 4-pack 1386100000 1386101000
BioBLU® 0.3c 100 mL - 250 mL Open pipe 1x pitched-blade Optical 4-pack 1386100200 1386101500
BioBLU® 0.3sc 100 mL - 250 mL Open pipe 1x 8-blade Optical 4-pack 1386100600 1386102000
BioBLU® 1c 320 mL - 1.25 L Open pipe 1x pitched-blade Standard 4-pack 1386110000 1386111000
BioBLU® 1c 320 mL - 1.25 L Open pipe 1x pitched-blade Optical 4-pack 1386110400 1386112000
BioBLU® 1c 320 mL - 1.25 L Open pipe 2x pitched-blade Standard 4-pack 1386110100 1386111100
BioBLU® 1c 320 mL - 1.25 L Open pipe 2x pitched-blade Optical 4-pack 1386110500 1386112100
BioBLU® 3c 1.25 L - 3.75 L Microsparger 1x pitched-blade Optical 1-pack 1386000100 1386124000
BioBLU® 3c 1.25 L - 3.75 L Macrosparger 1x pitched-blade Optical 1-pack 1386000300 1386125000
BioBLU® 3c 1.25 L - 3.75 L Microsparger 2x pitched-blade Optical 1-pack 1386120000 1386126000
BioBLU® 3c 1.25 L - 3.75 L Macrosparger 2x pitched-blade Optical 1-pack 1386121000 1386127000
BioBLU® 5p 3.75 L Microsparger Packed bed Optical 1-pack M1363-0119 –
BioBLU® 5p 3.75 L Macrosparger Packed bed Optical 1-pack M1363-0133 –
BioBLU® 10c 3.5 L -10 L Microsparger 1x pitched-blade Optical 1-pack 1386140000 1386140300
BioBLU® 10c 3.5 L -10 L Macrosparger 1x pitched-blade Optical 1-pack 1386141000 1386141300
BioBLU® 50c 18 L - 40 L Microsparger 1x pitched-blade Optical 1-pack M1363-0131 1386162000
BioBLU® 50c 18 L - 40 L Macrosparger 1x pitched-blade Optical 1-pack M1363-0129 1386163000

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
60

Bioreactors

BioBLU® c Single-Use Bioreactors

Technical specifications
Model BioBLU® 0.3c BioBLU® 0.3sc BioBLU® 1c
Bioreactors
Sterilization Irradiated by > 15 kGy (β) or > 25 kGy (X-ray).
SAL-level 10-6 for X-ray irradiated BioBLU® Single-Use Bioreactors
Working volume (total) 100 – 250 mL (380 mL) 100 – 250 mL (380 mL) 320 mL – 1.25 L (1.8 L)3)
Material vessel1) PS, PC (USP Class VI) PS, PC (USP Class VI) PS, PC (USP Class VI)
Material tubing Silicone, C-Flex2) Silicone, C-Flex2) Silicone, C-Flex2)
Head plate ports 2x Pg 13.5, 1x harvest tube, 1x Pg 13.5, 1x harvest tube, 3x Pg 13.5, 1x harvest tube,
1x thermowell, 1x sample 1x thermowell, 1x sample 1x thermowell, 1x sample
port, 1x liquid addition port, 1x liquid addition port, 2x liquid addition
overlay, 1x liquid addition overlay, 1x liquid addition overlay, 2x liquid addition
submerged, 1x DO sensor port submerged, 1x DO sensor port submerged, 1x DO sensor port
with permeable membrane, with permeable membrane, with permeable membrane,
1x gas sparge, 1x gas overlay, 1x optical pH option, 1x gas 1x gas sparge, 1x gas overlay,
1x exhaust sparge, 1x gas overlay, 1x exhaust
1x exhaust
Agitation
Drive Magnetic overhead drives Magnetic overhead drives Magnetic overhead drives
Impellers Pitched-blade 1x 8-blade Pitched-blade
Recommended agitation speed 20 – 500 rpm 20 – 200 rpm 30 – 600 rpm
Gassing
Gas supply Sparger and/or overlay Sparger and/or overlay Sparger and/or overlay
Monitoring and Control
Sensor length 120 mm 120 mm 220 mm
Temperature sensor Pt100 RTD (not included) Pt100 RTD (not included) Pt100 RTD (not included)
Max. operating temperature 40 °C 40 °C 40 °C
pH sensor Optical pH or standard glass Optical pH or standard glass Optical pH or standard glass
sensor (120 mm) sensor (120 mm) sensor (220 mm)
DO Sensor Polarographic DO - 4.7/118 mm Polarographic DO - 4.7/118 mm Polarographic DO - 4.7/229 mm

Exhaust condensation Liquid-free cooling (Peltier) Liquid-free cooling (Peltier) Liquid-free cooling (Peltier)/
water-cooled
1)
PC = Polycarbonate, PS = Polystyrene2) C-Flex is for optical pH version only.3) 425 mL minimal working volume when used with vessel stand and heating blanket

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Bioreactors

BioBLU® 3c BioBLU® 10c BioBLU® 50c BioBLU® 5p

Irradiated by > 15 kGy (β) or > 25 kGy (X-ray). Pre-sterilized (15 kGy
SAL-level 10-6 for X-ray irradiated BioBLU® Single-Use Bioreactors β-irradiated)
1.25 – 3.75 L (5 L) 3.3 – 10 L (13.3 L) 18 – 40 L (50 L) 3.75 L (5 L)
PC (USP Class VI) PC (USP Class VI) PS, PC (USP Class VI) PS, PC (USP Class VI)
Silicone, C-Flex Silicone, C-Flex Silicone, C-Flex Silicone, C-Flex
2x Pg 13.5, 1x harvest tube, 2x Pg 13.5 (1x 19 mm to 1x Pg 13.5, 1x harvest tube, 1x Pg 13.5, 1x harvest tube,
1x thermowell, 1x sample port, Pg 13.5), 1x harvest tube, 1x thermowell, 1x sample 1x thermowell, 1x sample port,
3x liquid addition overlay, 1x thermowell, 1x sample port, port, 3x liquid addition 3x liquid addition overlay, 1x DO
1x liquid addition submerged, 3x liquid addition overlay, overlay, 1x DO sensor port sensor port with permeable
1x DO sensor port with 1x liquid addition submerged, with permeable membrane, membrane, 1x optical pH sensor
permeable membrane, 1x optical 1x DO sensor port with 1x optical pH sensor port, port, 1x gas sparge, 1x gas
pH option, 1x gas sparge, 1x gas permeable membrane, 1x optical 1x gas sparge, 1x gas overlay, overlay, 1x exhaust
overlay, 1x exhaust pH option, 1x gas sparge, 1x gas 1x exhaust
overlay, 1x exhaust

Magnetic overhead drives Magnetic overhead drives Magnetic overhead drives Magnetic overhead drives
Pitched-blade Pitched-blade Pitched-blade Packed-bed
25 – 200 rpm 25 – 200 rpm 25 – 150 rpm 25 – 200 rpm

Sparger and/or overlay Sparger and/or overlay Sparger and/or overlay Sparger and/or overlay

225 mm 355 mm 526 mm 120 mm

Pt100 RTD (not included) Pt100 RTD (not included) Pt100 RTD (not included) Pt100 RTD (not included)
40 °C 40 °C 40 °C 40 °C
Optical pH or standard glass Optical pH or standard glass Optical pH or standard glass Optical pH or standard glass
sensor (220 mm) sensor (325 mm) sensor (625 mm) sensor (120 mm)
Polarographic or optical DO - Polarographic or optical DO - Polarographic DO - 12/526 mm Polarographic DO - 12/120 mm
12/225 mm 12/355 mm
Electric heater band, Peltier or Electric heater band Electric heater band Electric heater band, Peltier or
Water-cooled Water-cooled

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
62

Bioreactors

BioBLU® f Single-Use Bioreactors

Description Product features

Eppendorf BioBLU Single-Use Bioreactors combine all the >> Single-use, stirred-tank, rigid-walled bioreactors available in sizes
advantages of single-use technology with the trusted performance ranging from 65 mL to 3.75 L working volume
and scalability of a stirred-tank design. Single-use bioreactors >> Designed for high-density fermentation processes
eliminate labor-intensive cleaning, improve turn-around time, and >> Multiple Rushton-type impellers for efficient mixing and mass
reduce overall costs. BioBLU f bioreactors are specifically designed transfer
for robust microbial applications using bacteria, yeasts, and fungi. >> Sealed magnetic drive with fully enclosed bearings maintain vessel
sterility
>> Baffles for excellent mixing and mass transfer
Applications >> Bioreactors are assembled with sparger, gas filters for inlet and
>> Bacteria, yeasts, and fungi exhaust as well as penetrations for pH, DO, temperature, liquid
>> Suitable for high-cell density fermentation additions, sampling, and harvest
>> For use with Eppendorf benchtop bioreactor systems
>> Adaptor kits for legacy bioreactor controllers available

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 63

Bioreactors

Microbiology on the fast track - with the BioBLU f Single-Use Bioreactors.

Ordering information
Order no. Order no.
Description (β-irradiated) (X-ray irradiated)
Vessel Working Volume Sparger Impeller pH Quantity
BioBLU® 0.3f 65 mL - 250 mL Open pipe 2x Rushton-type Standard 4-pack 1386100100 1386101100
BioBLU® 1f 250 mL - 1.25 L Open pipe 2x Rushton-type Standard 4-pack 1386110200 1386113000
BioBLU® 1f 250 mL - 1.25 L Open pipe 3x Rushton-type Standard 4-pack 1386110300 1386113100
Order no.
(Autoclavable)
BioBLU® 3f 1.25 L - 3.75 L Macrosparger 3x Rushton-type Standard 1-pack 1386000900 –

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
64

Bioreactors

BioBLU® f Single-Use Bioreactors

Technical specifications
Model BioBLU® 0.3f
Application Microbiology
Bioreactors
Sterilization Irradiated by > 15 kGy (β) or > 25 kGy (X-ray).
SAL-level 10-6 for X-ray irradiated BioBLU® Single-Use Bioreactors
Working volume (total) 65 – 250 mL (380 mL)
Material vessel1) PS, PC (USP Class VI)
Material tubing Silicone
Head plate ports 2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 1x liquid
addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust

Agitation
Drive Magnetic overhead drives
Impellers Rushton-type
Recommended agitation speed 20 – 2,000 rpm
Gassing
Gas supply Sparger
Monitoring and Control
Sensor length 120 mm
Temperature sensor Pt100 RTD (not included)
Max. operating temperature 45 °C
pH sensor Standard glass sensor (120 mm)
DO Sensor Polarographic DO - 4.7/118 mm
Exhaust condensation Liquid-free cooling (Peltier)
1)
PC = Polycarbonate, PS = Polystyrene

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 65

Bioreactors

BioBLU® 1f BioBLU® 3f
Microbiology Microbiology

Irradiated by > 15 kGy (β) or > 25 kGy (X-ray). Autoclavable, no pre-sterilization

SAL-level 10-6 for X-ray irradiated BioBLU® Single-Use Bioreactors
250 mL – 1.25 L (1.8 L) 1.25 L – 3.75 L (5 L)
PS, PC (USP Class VI) PC (USP Class VI)
Silicone Silicone
3x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid 4x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid
addition overlay, 2x liquid addition submerged, 1x DO sensor port addition overlay, 1x liquid addition submerged, 1x gas sparge, 1x
with permeable membrane, 1x gas sparge, 1x exhaust, 4x baffles gas overlay, 1x exhaust, 4x baffles
(incl. cooling water connection)

Magnetic overhead drives Magnetic overhead drives

Rushton-type Rushton-type
100 – 1,500 rpm 25 – 1,200 rpm

Sparger Sparger and/or overlay

220 mm 220 mm
Pt100 RTD (not included) Pt100 RTD (not included)
45 °C 45 °C
Standard glass sensor (220 mm) Standard glass sensor (225 mm)
Polarographic DO - 4.7/229 mm Polarographic DO - 12/220 mm
Liquid-free cooling (Peltier)/water-cooled Water-cooled

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
66

Bioreactors

BioBLU® Single-Use Bioreactor Adaptor Kits

Description
Single-use bioreactor adaptor kits convert existing autoclavable bioreactor controllers for use with Eppendorf BioBLU Single-Use Bioreactors.
Easy to install kits provide all the necessary equipment for conversion.

Product features
>> Adapt your existing Eppendorf DASbox, DASGIP, and BioFlo/
CelliGen autoclavable systems for use with Eppendorf BioBLU
Single-Use Bioreactors
>> Provides all the benefits of single-use technology with minimal
upfront investment
>> Kits include all parts necessary for conversion of the respective
system, such as motor adaptor, exhaust treatment, and more
>> BioBLU Single-Use Bioreactors sold separately

Ordering information
Description Order no.
Adaptor Kit: BioBLU® Single-Use Vessels, for DASbox®
for 4 BioBLU® 0.3, Peltier condensers 78532298
Adaptor Kit: BioBLU® Single-Use Vessels, for DASGIP® Bioblock
for 4 BioBLU® 1f, Peltier condensers 78532295
for 4 BioBLU® 1c, Peltier condensers 78532299
for 4 BioBLU® 1f, water-based condensers 78532330
for 4 BioBLU® 1c, water-based condensers 78532329
Adaptor Kit: BioBLU® Single-Use Vessels
for DASGIP® benchtop system, for 4 BioBLU® 1c, Peltier condensers 78532328
for DASGIP® benchtop system, 115 V, for 4 BioBLU® 3c/5c/5p, Peltier condensers 78532296
for DASGIP® benchtop system, 230 V, for 4 BioBLU® 3c/5c/5p, Peltier condensers 78532297
Adaptor Kit: BioBLU® Single-Use Vessels, for DASGIP® benchtop system
for 4 BioBLU® 1c, water-based condensers 78532334
115 V, for 4 BioBLU® 3c/5c/5p, water-based condensers 78532331
230 V, for 4 BioBLU® 3c/5c/5p, water-based condensers 78532333
Adaptor Kit: BioBLU® Single-Use Vessel, for BioFlo® 320
100 – 240 V, BioBLU® 3c/5c/5p M1386-9943
100 – 240 V, BioBLU® 3f M1386-9923
100 – 240 V, BioBLU® 10c/14c M1386-9963
100 – 240 V, BioBLU® 50c M1386-9983

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 67

Bioreactors

Ordering information
Description Order no.
Adaptor Kit: BioBLU® Single-Use Vessel, for BioFlo®/CelliGen 115
100 – 240 V, BioBLU® 3c/5c/5p M1386-9940
100 – 240 V, BioBLU® 3f, for direct-drive motor of 1 L or 2 L vessel M1386-9920
100 – 240 V, BioBLU® 3f, for direct-drive motor of 5 L or 10 L vessel M1386-9921
100 – 240 V, BioBLU® 10c/14c M1386-9960
100 – 240 V, BioBLU® 50c M1386-9980
Adaptor Kit: BioBLU® Single-Use Vessel, for CelliGen 310
100 – 120 V, BioBLU® 3c/5c/5p M1386-9941
100 – 120 V, BioBLU® 10c/14c M1386-9961
100 – 120 V, BioBLU® 50c M1386-9981
200 – 240 V, BioBLU® 3c/5c/5p M1386-9942
200 – 240 V, BioBLU® 10c/14c M1386-9962
200 – 240 V, BioBLU® 50c M1386-9982
Adaptor Kit: BioBLU® Single-Use Vessel, for BioFlo® 310, 100 – 240 V, BioBLU® 3f M1386-9922
Adaptor kits are designed to adapt an existing unit in the field to single-use bioreactors. For information on single-use bioreactor bundles in combination with a new controller, please refer to page 91 and 99.

Accessories
Description Order no.
Adaptor
single-use compression fitting, for Pg 13.5 port, pack of 10 1386 010 200
single-use blind plug, for Pg 13.5 port, pack of 10 1386 010 300
single-use septum kit, for Pg 13.5 port, pack of 10 1386 010 100
single-use tri-port, for Pg 13.5 port, pack of 10 1386 010 000
OP-76 Optical pH Module 1) M1376-1001
DASGIP® EGC4 Exhaust Gas Condenser Controller, for 4 Peltier actuators, 110 – 240 V/50/60 Hz 76DGEGC4
Vessel Stand
BioBLU® 1 M1379-4000
BioBLU® 3 M1386-0300
BioBLU® 10 M1386-0302
Exhaust Condenser, Peltier
for 1 BioBLU® 0.3c/f Single-Use Vessel 76DXCONDSU
for 1 BioBLU® 1c Single-Use Vessel 76DGCONDSU1C
for 1 BioBLU® 1f Single-Use Vessel 76DGCONDSU1F
for 1 BioBLU® 3c/5c/5p Single-Use Vessel 76DGCONDSU5C
Y-Connector, with 1/4 in CPC connector P0620-0947
Vessel Connection Kit, for BioBLU® M1363-0112
Level/Foam Sensor, for 1 single-use vessel, including triple port, for 1 vessel, Pg 13.5 with two sensors (150 mm, 76DGLVLSU
220 mm) and media addition
Cooling Finger, for BioBLU® 3f 2) M1386-0555
1)
M1376-1001: For optical pH sensor cables, see page 161, 2) Requires M1287-5030

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
68

Bioreactors

DASbox® Mini Bioreactor

Description Product features

The DASbox Mini Bioreactor is an industry-standard autoclavable >> Industry-standard design for excellent scalability and
glass vessel featuring a multi port stainless steel head plate reproducibility in both cell culture and microbiology
and a powerful direct overhead drive. With working volumes of >> Stainless steel head plate, fully instrumented with standard sensors
60 – 250 mL it is perfectly suited for process development in cell for precise measurement and control of temperature, pH, DO, and
culture and microbial applications and ready for use with the level
Eppendorf DASbox. >> Small working volumes of 60 – 250 mL saving valuable resources
>> Powerful direct overhead drive with marine impeller (cell culture)
or two Rushton-type impellers (microbiology)
>> Liquid-free exhaust condenser with easy handling by automatic
slide-in activation and slide-out deactivation mode

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 69

Bioreactors

Technical specifications
Model Cell culture Microbiology
Standard set-up DASbox DASbox
Dimensions (W × D × H) 90 × 90 × 360 mm 90 × 90 × 360 mm
Bioreactors
Sterilization Autoclavable Autoclavable
Working volume (total) 60 – 250 mL (350 mL) 60 – 250 mL (350 mL)
Material vessel1) Glass Glass
Head plate Stainless steel, GLS80 screw cap Stainless steel, GLS80 screw cap
Head plate ports 6x Pg 13.5, 2x dip tube long, 2x dip tube short, 1x 6x Pg 13.5, 2x dip tube long, 2x dip tube short,
thermowell 1x thermowell
Agitation
Drive Direct overhead drive Direct overhead drive
Impellers 1x marine 2x Rushton-type
Recommended agitation speed 20 – 2,500 rpm 20 – 2,500 rpm
Gassing
Gas supply Overlay and/or sparger Sparger
Monitoring and Control
Sensor length 120 mm 120 mm
Temperature sensor Pt100 RTD Pt100 RTD
Exhaust condensation Liquid-free (Peltier) Liquid-free (Peltier)
1)
PC = Polycarbonate, PS = Polystyrene

Contents of vessel kits

Cell culture/Stem cell culture Microbiology
Flat-bottom vessel with stainless  

steel head plate, screw cap and

o-ring
Overhead drive (20 - 2,500 rpm) Sold separately Sold separately
Sensors Sold separately Sold separately
Impeller  

Sparger assembly (dip tube) 

Sparger assembly (L-sparger) 

Peltier exhaust condenser with filter Sold separately Sold separately

Thermowell  

Sampling assembly  

Liquid addition tube (qty. 3)  

Septum kit  

Inlet filter  

Silicone tubing  

Tools (tubing clamp, hex wrench)  

■ = standard

Ordering information
DASbox® Mini Bioreactor
Application Working volume (total) Impellers Order no.
Cell culture 60 – 250 mL (350 mL) 1x marine 76DS0250ODSS
Microbiology 60 – 250 mL (350 mL) 2x Rushton-type 76SR0250ODLS
Stem cell culture 60 – 250 mL (350 mL) 1x 8-blade 76DX0250V01

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
70

Bioreactors

DASGIP® Bioblock / SciVario® twin Spinner Vessels

Description Product features

Eppendorf offers a line of advanced autoclavable spinner vessels >> Working volumes ranging from 250 mL – 1.5 L
suitable for temperature control with the compact DASGIP Bioblock >> Direct overhead drives with 30 – 1,250 rpm (100 – 1,600 rpm
and SciVario twin temperature control block. These overhead driven optional), pitched-blade impellers
spinner vessels feature a stainless steel head plate with standard >> Industry-standard sensors available for accurate monitoring and
ports, pitched-blade impellers, and two side arms. Covering a working control of temperature, pH, DO, level, and OD
volume range of 200 mL – 1.6 L they are perfectly suited for cell >> Two GL45 side arms
culture research and process development.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 71

Bioreactors

Technical specifications
Model 0.7 L 1L 1.5 L
Application Cell culture Cell culture Cell culture
Standard set-up DASGIP Bioblock/SciVario twin DASGIP Bioblock/SciVario twin DASGIP Bioblock/SciVario twin
Dimensions (W × D × H) 250 × 150 × 510 mm 250 × 150 × 560 mm 250 × 150 × 610 mm
Bioreactors
Sterilization Autoclavable Autoclavable Autoclavable
Working volume (total) 250 mL – 700 mL (1.5 L) 350 mL – 1.0 L (1.9 L) 350 mL – 1.5 L (2.3 L)
Material vessel1) Glass Glass Glass
Head plate Stainless steel, screw cap Stainless steel, screw cap Stainless steel, screw cap
Head plate ports 1x M30, 7x Pg 13.5, 1x M30, 7x Pg 13.5, 1x M30, 7x Pg 13.5,
1x thermowell 1x thermowell 1x thermowell
Agitation
Drive Direct overhead drive Direct overhead drive Direct overhead drive
Impellers 1x pitched blade 2x pitched-blade 2x pitched-blade
Recommended agitation speed 30 – 1,250 rpm 30 – 1,250 rpm 30 – 1,250 rpm
Gassing
Gas supply Overlay and/or sparger Overlay and/or sparger Overlay and/or sparger
Monitoring and Control
Sensor length 220 mm 220 mm 320 mm
Temperature sensor Pt100 RTD Pt100 RTD Pt100 RTD
Exhaust condensation Water-cooled Water-cooled Water-cooled
1)
PC = Polycarbonate, PS = Polystyrene

Contents of Vessel Kits

Contents
Flat-bottom vessel with sidearms, 

stainless steel head plate and screw caps

Overhead drive (30 - 1,250 rpm) Sold separately
Sensors Sold separately
Impeller 

Sparger assembly (dip tube) 

Exhaust condenser with filter Sold separately

Thermowell 

Sampling assembly 

Triple port with liquid addition tubes 

Septum kit 

Inlet filter 

Silicone tubing 

Tools (tubing clamp, hex wrench) 

■ = standard

Ordering information
DASGIP® Bioblock / SciVario® twin Spinner Vessels
Vessel Volume Working volume (total) Impellers Order no.
0.7 L 250 mL – 700 mL (1.5 L) 1x pitched blade 76DS0700ODSS
1L 350 mL – 1.0 L (1.9 L) 2x pitched-blade 76DS1000ODSS
1.5 L 350 mL – 1.5 L (2.3 L) 2x pitched-blade 76DS1500ODSS

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
72

Bioreactors

DASGIP® Bioblock / SciVario® twin Stirrer Vessels

Description Product features

Eppendorf offers a line of advanced autoclavable stirrer vessels >> Working volumes ranging from 200 mL – 1.8 L
suitable for temperature control with the compact DASGIP Bioblock >> Direct overhead drives with 100 – 1,600 rpm (30 – 1,250 rpm
and SciVario twin temperature control block. These overhead-driven optional), Rushton-type impellers
stirrer vessels feature a stainless steel head plate with standard ports >> Industry-standard sensors available for precise monitoring and
and Rushton-type impellers. Covering a working volume range of control of temperature, pH, DO, redox potential, level, and OD
200 mL – 1.8 L they are perfectly suited for microbial research and
process development.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 73

Bioreactors

Technical specifications
Model 1L 1.5 L 1.8 L
Application Microbiology Microbiology Microbiology
Standard set-up DASGIP Bioblock/SciVario twin DASGIP Bioblock/SciVario twin DASGIP Bioblock/SciVario twin
Dimensions (W × D × H) 110 × 150 × 500 mm 110 × 150 × 570 mm 110 × 150 × 610 mm
Bioreactors
Sterilization Autoclavable Autoclavable Autoclavable
Working volume (total) 200 mL – 1.0 L (1.3 L) 400 mL – 1.5 L (1.8 L) 400 mL – 1.8 L (2.1 L)
Material vessel1) Glass Glass Glass
Head plate Stainless steel, screw cap Stainless steel, screw cap Stainless steel, screw cap
Head plate ports 1x M30, 7x Pg 13.5, 1x 1x M30, 7x Pg 13.5, 1x 1x M30, 7x Pg 13.5, 1x
thermowell thermowell thermowell
Agitation
Drive Direct overhead drive Direct overhead drive Direct overhead drive
Impellers 2x Rushton-type 2x Rushton-type 3x Rushton-type
Recommended agitation speed 100 – 1,600 rpm 100 – 1,600 rpm 100 – 1,600 rpm
Gassing
Gas supply Sparger Sparger Sparger
Monitoring and Control
Sensor length 220 mm 220 mm 320 mm
Temperature sensor Pt100 RTD Pt100 RTD Pt100 RTD
Exhaust condensation Water-cooled Water-cooled Water-cooled
1)
PC = Polycarbonate, PS = Polystyrene

Contents of Vessel Kits

Contents
Flat-bottom vessel with stainless 

steel head plate and screw caps

Overhead drive (100 - 1,600 rpm) Sold separately
Baffle assembly Sold separately (1 L and 1.5 L vessels only)
Sensors Sold separately
Impellers 

Sparger assembly (L-sparger) 

Exhaust condenser with filter Sold separately

Thermowell 

Sampling assembly 

Triple port with liquid addition tubes 

Septum kit 

Inlet filter 

Silicone tubing 

Tools (tubing clamp, hex wrench) 

■ = standard

Ordering information
DASGIP® Bioblock / SciVario® twin Stirrer Vessels
Vessel Volume Working volume (total) Impellers Order no.
1L 200 mL – 1.0 L (1.3 L) 2x Rushton-type 76SR0700ODLS
1.5 L 400 mL – 1.5 L (1.8 L) 2x Rushton-type 76SR1000ODLS
1.8 L 400 mL – 1.8 L (2.1 L) 3x Rushton-type 76SR1500ODLS

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
74

Bioreactors

SciVario® twin Benchtop Bioreactors for Cell Culture

Description Product features

DASGIP Benchtop Bioreactors feature an autoclavable glass >> Working volumes of 750 mL – 2.7 L and 850 mL – 3.8 L
body and a stainless steel head plate. 16 industry-standard >> Direct overhead drives with 30 – 1,250 rpm (100 – 1,600 rpm
ports, direct overhead drives and pitched-blade impellers ensure optional), pitched-blade impellers
optimal conditions for advanced cell culture research and process >> Industry-standard sensors available for precise monitoring and
development. All stainless-steel parts are laser-labelled with part control of temperature, pH, DO, level, and OD
numbers and have certificates of origin available.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 75

Bioreactors

Technical specifications
Model 2.5 L 3.5 L
Application Cell culture Cell culture
Standard set-up SciVario twin SciVario twin
Dimensions (W × D × H) 190 × 190 × 580 mm 190 × 190 × 660 mm
Bioreactors
Sterilization Autoclavable Autoclavable
Working volume (total) 750 mL – 2.7 L (3.1 L) 850 mL – 3.8 L (4.3 L)
Material vessel1) Glass Glass
Head plate Stainless steel Stainless steel
Head plate ports 1x M30, 8x M18x1.5, 8x D6 1x M30, 8x M18x1.5, 8x D6
Agitation
Drive Direct overhead drive Direct overhead drive
Impellers 2x pitched-blade 2x pitched-blade
Recommended agitation speed 30 – 1,250 rpm 30 – 1,250 rpm
Gassing
Gas supply Overlay and/or sparger Overlay and/or sparger
Monitoring and Control
Sensor length 220 mm 320 mm
Temperature sensor Pt100 RTD Pt100 RTD
Exhaust condensation Water-cooled Water-cooled
1)
PC = Polycarbonate, PS = Polystyrene

Contents of Vessel Kits

Contents
Dished-bottom vessel with 

stainless steel head plate

Vessel stand 

Overhead drive (30 - 1,250 rpm) Sold separately

Sensors Sold separately
Impeller 

Sparger assembly L-sparger 

Exhaust condenser with filter Sold separately

Thermowell 

Sampling assembly 

Triple port with liquid addition 

tubes
Septum kit 

Inlet filter 

Silicone tubing 

Tools (tubing clamp, hex 

wrench)
■ = standard

Ordering information
DASGIP® Benchtop Bioreactors for Cell Culture
Vessel Volume Working volume (total) Impellers Order no.
2.5 L 750 mL – 2.7 L (3.1 L) 2x pitched-blade 76DR03C
3.5 L 850 mL – 3.8 L (4.3 L) 2x pitched-blade 76DR04C

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
76

Bioreactors

SciVario® twin Benchtop Bioreactors for Microbiology

Description Product features

DASGIP Benchtop Bioreactors feature an autoclavable glass >> Working volumes of 700 mL – 2.7 L and 800 mL – 3.8 L
body and a stainless steel head plate. 16 industry-standard >> Direct overhead drives with 100 – 1,600 rpm (30 – 1,250 rpm
ports, direct overhead drives and Rushton-type impellers ensure optional), Rushton-type impellers
optimal conditions for advanced microbial research and process >> Industry-standard sensors available for precise monitoring and
development. All stainless-steel parts are laser-labelled with part control of temperature, pH, DO, redox potential, level, and OD
numbers and have certificates of origin available.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 77

Bioreactors

Technical specifications
Model 2.5 L 3.5 L
Application Microbiology Microbiology
Standard set-up SciVario twin SciVario twin
Dimensions (W × D × H) 190 × 190 × 580 mm 190 × 190 × 660 mm
Bioreactors
Sterilization Autoclavable Autoclavable
Working volume (total) 700 mL – 2.7 L (3.2 L) 800 mL – 3.8 L (4.3 L)
Material vessel1) Glass Glass
Head plate Stainless steel Stainless steel
Head plate ports 1x M30, 8x M18x1.5, 4x D6, 4x baffles 1x M30, 8x M18x1.5, 4x D6, 4x baffles
Agitation
Drive Direct overhead drive Direct overhead drive
Impellers 2x Rushton-type 3x Rushton-type
Recommended agitation speed 100 – 1,600 rpm 100 – 1,600 rpm
Gassing
Gas supply Sparger Sparger
Monitoring and Control
Sensor length 220 mm 320 mm
Temperature sensor Pt100 RTD Pt100 RTD
Exhaust condensation Water-cooled Water-cooled
1)
PC = Polycarbonate, PS = Polystyrene

Contents of Vessel Kits

Contents
Dished-bottom vessel with stainless 

steel head plate

Vessel stand 

Overhead drive (100 - 1,600 rpm) Sold separately

Baffle assembly 

Sensors Sold separately

Impeller 

Sparger (L-sparger) 

Exhaust condenser with filter Sold separately

Thermowell 

Sampling assembly 

Triple port with liquid addition tubes 

Septum kit 

Inlet filter 

Silicone tubing 

Tools (tubing clamp, hex wrench) 

■ = standard

Ordering information
DASGIP® Benchtop Bioreactors for Microbiology
Vessel Volume Working volume (total) Impellers Order no.
2.5 L 700 mL – 2.7 L (3.2 L) 2x Rushton-type 76DR03F
3.5 L 800 mL – 3.8 L (4.3 L) 3x Rushton-type 76DR04F

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
78

Bioreactors

DASbox®, DASGIP®,
and SciVario twin Bioreactor Accessories

Head Plates
>> Stainless steel, electropolished
>> All wetted parts laser-labeled with part numbers and material certificates

Model Stainless Steel Head Plate Stainless Steel Head Plate Stainless Steel Head Plate
Order no. 78107301 78107157 78107249
Diameter outer (O.D.) 77 mm 100 mm 190 mm
DASbox® MiniBioreactor 

DASGIP® Bioblock/SciVario® twin Spinner 

0.7 L/Stirrer 1 L
DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

D4 ports 4
D6 ports 1 8
M18 ports 8
M30 ports 1 1
Pg 13.5 ports 6 7
Thermowell included 1 - -
■ = standard

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 79

Bioreactors

Compression Fittings and Triple Ports

>> Available with Pg 13.5 and M18 threads
>> Inner Diameter (I.D.) 4 mm, 6 mm or 12 mm

Ordering information
Description Order no.
Compression Fitting, complete, with Pg 13.5 male thread
I.D. 4 mm 78532282
I.D. 6 mm 78532283
Compression Fitting, complete, with Pg 13.5 male thread, I.D. 12 mm 78532284
Compression Fitting, complete, with M18x1.5 male thread
I.D. 4 mm 78532279
I.D. 6 mm 78532280
Compression Fitting, complete, with M18x1.5 male thread, I.D. 12 mm 78532281
Adaptor
Pg 13.5 female thread to M18x1.5 male thread 77102016

Ordering information
Description Order no.
Back Ferrule, for compression fitting, PFA
O.D. 4 mm 78706352
O.D. 6 mm 78706354
O.D. 12 mm 78706356
Front Ferrule, for compression fitting, PFA
O.D. 4 mm 78706351
O.D. 6 mm 78706353
O.D. 12 mm 78706355

Ordering information
Description Order no.
Triple Port, Pg 13.5 thread, 3 tubes with O.D. 4 mm x L 85 mm, all parts included 78706414
Triple Port, M18 x 1.5 thread, 3 tubes with O.D. 4 mm x L 85 mm, all parts included 77102018
Triple Port, Pg 13.5 thread, welded, 3 tubes with O.D. 4 x Li 219 mm 78706587
Triple Port, Pg 13.5 thread, welded, 2 tubes with O.D. 4 x Li 219 mm, 1 tube with O.D. 4 x Li 21 mm 78706588
Triple Port, Pg 13.5 thread, welded, 3x O.D. 4 x Li 21 mm 78109071
Triple Port, Pg 13.5 thread, welded, 2 tubes with O.D. 4 x Li 21 mm, 1 tube with O.D. 4 x Li 219 mm 78109075

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
80

Bioreactors

DASbox®, DASGIP®,
and SciVario twin Bioreactor Accessories

Blind Plugs and Septa

>> Stainless steel
>> Pg 13.5, M18 and M6 threads
>> Silicon septa available for addition, inoculation or sampling

Ordering information
Description Order no.
Blind Plug, stainless steel, all parts included
Pg 13.5 thread 78532300
M6 male thread 77102020
M18 x 1.5 thread 77102017
Septum Holder, stainless steel, with septum
M18 x 1.5 male, I.D. 12 mm 77102019
Pg 13.5 male, I.D. 12 mm 77102006
Septum, silicone rubber
Pg 13.5, O.D. 18 mm/I.D. 12 mm 78106309
GL45 78106305
Septum, silicone rubber, PTFE-coated, GL45 78106306
Adaptor
Pg 13.5 female thread to M18x1.5 male thread 77102016

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 81

Bioreactors

O-Rings
>> O-Rings are available in different materials (VMQ silicone, NBR rubber) and sizes.

Ordering information
Description Order no.
O-Ring, VMQ 70/Si 820, red, 4 x 1.5 (I.D. x d) 78706417
O-Ring, VMQ 70/Si 820, red, 5 x 1.5 (I.D. x d) 78706429
O-Ring, VMQ 70/Si 820, red, 6 x 1.5 (I.D. x d) 78706416
O-Ring, VMQ 70/Si 820, red, 6 x 2 (I.D. x d) 78706407
O-Ring, VMQ 70/Si 820, red, 12 x 1.5 (I.D. x d) 78706419
O-Ring, VMQ 70/Si 820, red, 14 x 2 (I.D. x d) 78706406
O-Ring, VMQ 70/Si 820, red, 20 x 2 (I.D. x d) 78706458
O-Ring, VMQ 70/Si 820, red, 24 x 2 (I.D. x d) 78706440
O-Ring, VMQ 70/Si 820, red, 31 x 2.5 (I.D. x d) 78706439
O-Ring, VMQ 70/Si 820, red, 68 x 3 (I.D. x d) 78201138
O-Ring, VMQ 70/Si 820, red, 88 x 3 (I.D. x d) 78706408
O-Ring, VMQ 70/Si 820, red, 135 x 4 (I.D. x d) 78706460
O-Ring, VMQ 70/Si 820, red, 4 x 1 (I.D. x d) 78706415
O-Ring, VMQ 50/Si 50, blue, 8 x 1.5 (I.D. x d) 78706465
O-Ring, VMQ 65/Si 840, blue, 18 x 2.5 (I.D. x d) 78706447
O-Ring, NBR70/P583, black, 166.75 x 2 (I.D. x d) 78706478

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
82

Bioreactors

DASbox®, DASGIP®,
and SciVario twin Bioreactor Accessories

Valves
>> Easy and efficient sampling from any bioreactor
>> Self-sealing Luer lock connector

Ordering information
Description Order no.
Sampling Accessory, with swabable valve 78510145
Sample Valve, autoclavable, male Luer lock and swabable female Luer lock 78200077
Cap for Sampling Valve, autoclavable, male Luer lock 78200087
One Way Valve, with Luer lock female inlet and male outlet 78200078

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 83

Bioreactors

Stainless Steel Pipes

>> Stainless steel, electropolished
>> Various diameters and lengths available
>> For sampling, harvesting, submerged gassing and liquid addition

Model Stainless Steel Pipes, with barb, OD 4mm/ID 2 mm

Order no. 78107326 78107023 78107102 78107146 78107178
Length 180 mm 225 mm 270 mm 320 mm 370 mm
DASbox® MiniBioreactor 

DASGIP® Bioblock/ 

SciVario® twin Spinner 0.7 L/Stirrer 1 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard, o = optional

Model M6 Port Assembly, OD 6 mm/ID 2 mm Harvest Tube, OD 6 mm

Order no. 77102060 77102061 78107379 78107380
Length 281 mm 356 mm 320 mm 410 mm
DASbox® MiniBioreactor
DASGIP® Bioblock/
SciVario® twin Spinner 0.7 L/Stirrer 1 L
DASGIP® Bioblock/
SciVario® twin Spinner 1 L/Stirrer 1.5 L
DASGIP® Bioblock/
SciVario® twin Spinner 1.5 L/Stirrer 1.8 L
SciVario® twin Benchtop Bioreactors 2.5 L o o
SciVario® twin Benchtop Bioreactors 3.5 L o o
DASGIP® PhotoBioreactors 1.0 L
DASGIP® PhotoBioreactors 2.5 L o
■ = standard, o = optional

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
84

Bioreactors

DASbox®, DASGIP®,
and SciVario twin Bioreactor Accessories

Thermowells
>> Easy and sterile insertion of temperature sensors
>> Various lenghts available

Model Thermowell, M6, stainless steel

Order no. 77102027 77102003 77102029 77102030 77102031
Inner diameter (I.D.) 2 mm 2 mm 2 mm 2 mm 2 mm
Inner length 163 mm 188 mm 238 mm 263 mm 313 mm
Length 200 mm 225 mm 275 mm 300 mm 350 mm
DASGIP® Benchtop Spinner 0.5 L  

DASGIP® Benchtop Spinner 1 L 

DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock Spinner 0.7 L 

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 85

Bioreactors

Impellers
>> Rushton-type, pitched-blade and marine impellers for microbiology
and cell culture
>> Various sizes and shaft lengths available

6-Blade Rushton-Type 3-Blade Impeller, 8-Blade Impeller,

Model Impeller, stainless-steel 30° pitch, stainless steel 60° pitch, stainless steel
Order no. 78107304 78100557 78532236 78107325 78100604 78100605
Inner diameter (I.D.) 5 mm 8 mm 8 mm 5 mm 5 mm 8 mm
Diameter outer (O.D.) 30 mm 46 mm 50 mm 30 mm 34 mm 53 mm
DASbox® MiniBioreactor   

DASGIP® Bioblock/   

SciVario® twin Spinner 0.7 L/Stirrer 1 L

DASGIP® Bioblock/   

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock/   

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L   

SciVario® twin Benchtop Bioreactors 3.5 L   

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard

Model DASGIP® Lipseal Stirrer Assembly

Order no. 78525116 78525130 78525119 78525118 78525123
Seat M30 M30 M30 M30 Pg 13.5
Stirrer shaft diameter 8 mm 8 mm 8 mm 8 mm 5 mm
Stirrer shaft inner length 186 mm 199 mm 245 mm 298 mm 112 mm
DASbox® MiniBioreactor 

DASGIP® Benchtop Spinner 1 L 

DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock Spinner 0.7 L 

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
86

Bioreactors

DASbox®, DASGIP®,
and SciVario twin Bioreactor Accessories
Caps for Vessels and Bottles
>> Various sizes available, closed or with ports

Ordering information
Description Order no.
Screw Cap, for O.D. 100 mm neck, PBT, with hole ID 90 mm without gasket 78903225
Screw Cap, for O.D. 80 mm neck, PBT, with hole I.D. 73 mm, without gasket 78903226
Screw Cap, GL45, PBT, with port I.D. 34 mm 78903224
Screw Cap, GL45, PBT, closed, including PTFE-coated silicone washer 78106122

Accessories
Description Order no.
Washer, silicone, PTFE-coated
for GL45, I.D. 32 mm, O.D. 42 mm, d 3 mm 78106307

Exhaust Condensers
>> Minimized evaporation during cultivation
>> DASGIP Cooling Water Distribution Unit allows for individual
operation of each exhaust condender

Model Condenser
Order no. 77102050
Diameter outer (O.D.) 30 mm
DASGIP® Bioblock/ 

SciVario® twin Spinner 0.7 L/Stirrer 1 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 87

Bioreactors

Cooling Fingers
>> Highly efficient cooling even in high cell density applications
>> Various lengths available

Model Cooling Finger Cooling Finger

Order no. 77102037 77102036
Inner length 240 mm 325 mm
Length 295 mm 380 mm
Diameter outer (O.D.) 12 mm 12 mm
DASGIP® Bioblock/ 

SciVario® twin Spinner 0.7 L/Stirrer 1 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard

DASGIP® Heat Blankets

>> For individual temperature control of DASGIP vessels
>> Heating power 100 W
>> Power supply 115 or 230 V

Ordering information
Description Order no.
Heat Blanket, for DASGIP® vessel 0.5 – 3L
with Pt100, 95 x 260 mm, 100 W, 230 VAC, CE/UL certified 78525275
with Pt100, 95 x 260 mm, 100 W, 115 VAC, CE/UL certified 78525276

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
88

Bioreactors

DASbox®, DASGIP®,
and SciVario twin Bioreactor Accessories

Spargers
>> For submerged gassing
>> Stainless steel

Model L-Sparger for DASGIP® Culture Vessel, stainless steel

Order no. 77102052 77102054 77102022 77102023
Length 180 mm 300 mm 300 mm 370 mm
Port D4 D4 D6 D6
Width 40 mm 42 mm 63 mm 63 mm
DASbox® MiniBioreactor 

DASGIP® Bioblock/ o 

SciVario® twin Spinner 0.7 L/Stirrer 1 L

DASGIP® Bioblock/ o 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

SciVario® twin Benchtop Bioreactors 2.5 L o 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L o 

DASGIP® PhotoBioreactors 2.5 L o 

■ = standard, o = optional

Model Stainless Steel Pipes, with barb, OD 4mm/ID 2 mm

Order no. 78107326 78107023 78107102 78107146 78107178
Length 180 mm 225 mm 270 mm 320 mm 370 mm
DASbox® MiniBioreactor 

DASGIP® Bioblock/ 

SciVario® twin Spinner 0.7 L/Stirrer 1 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1 L/Stirrer 1.5 L

DASGIP® Bioblock/ 

SciVario® twin Spinner 1.5 L/Stirrer 1.8 L

SciVario® twin Benchtop Bioreactors 2.5 L 

SciVario® twin Benchtop Bioreactors 3.5 L 

DASGIP® PhotoBioreactors 1.0 L 

DASGIP® PhotoBioreactors 2.5 L 

■ = standard

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 89

Bioreactors

Micro Sparger
>> For submerged gassing
>> Stainless Steel
>> Pore size 10 µm

Ordering information
Description Order no.
Micro Sparger, stainless steel, pore size 10 µm, O.D. 6 mm, with O.D. 4 mm pipe
L 284 mm, 90° tip 78530205
L 370 mm, 90° tip 78530206
L 245 mm 78530511
L 290 mm 78530512
L 340 mm 78530513

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
90

Bioreactors

BioFlo® 120 Bioreactors

Description Product features

The BioFlo 120 is a bench-scale bioreactor/fermentor system >> Autoclavable glass vessels featuring working volume range
perfectly suited for all levels of research and development. The between 0.4 – 10.5 L
system was designed to be flexible to meet the wide-ranging needs >> Bundles for use with BioBLU Single-Use Bioreactors
of scientists today. 24 interchangeable heat-blanketed and water- >> Consistent vessel geometries for scale-up processes
jacketed autoclavable vessels are available, along with BioBLU >> Fermentation vessel assemblies capable of high oxygen transfer
Single-Use Bioreactors ranging from 250 mL to 40 L working volume. rates, designed for maximum density microbial applications
>> Pick from 24 autoclavable vessel options for cell culture and
fermentation, including heat-blanketed and water-jacketed vessels
with direct or magnetic drive
>> Conversion bundles to keep using your legacy vessels
>> Rushton-type, pitched-blade, and marine impellers as well as
spinfilters available

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 91

Bioreactors

Vessel Specifications
Autoclavable vessels
Vessel 1L 2L 5L 10 L
Total volume 1.3 L 3.0 L 7.5 L 14.0 L
Working volume 0.4 – 1.0 L 0.8 – 2.2 L 2.0 – 5.6 L 4.0 – 10.5 L
Vessel type Water-jacketed or heat-blanketed
Material Borosilicate glass, 316L stainless steel
Impellers
Direct drive or magnetic drive Rushton-type, pitched blade, marine or spin filter
Autoclave dimensions
Heat-blanketed
Outer diameter (O.D.) 20.3 cm 20.3 cm 29.8 cm 29.8 cm
8.0 in 8.0 in 11.7 in 11.7 in
Height (without exhaust filter) 54.0 cm 54.0 cm 61.0 cm 68.0 cm
21.3 in 21.3 in 24.0 in 26.9 in
Water-jacketed
Outer diameter (O.D.) 24.1 cm 24.1 cm 29.8 cm 29.8 cm
9.5 in 9.5 in 11.7 in 11.7 in
Height (without exhaust filter) 48.9 cm 56.5 cm 64.8 cm 76.8 cm
19.3 in 22.3 in 25.5 in 30.3 in
Number of head plate ports
6 mm 1 6 7 7
12 mm 9 7 8 8
19 mm 0 0 1 1
Total 10 13 16 16
Recommended sensor lengths
(mm)
Sensor
pH (analog)1) 200 225 325 425
pH/redox (digital)1) 225 225 325 425
DO (analog)1) 160 220 320 420
DO (digital/optical)1) 220 220 320 420
Redox (analog)1) 200 225 325 425
CO2 (digital)1) 220 220 320 320
1)
Installation may require compression fitting for optimal fit and depth (M1273-5040), 2 x included with Vessel Connection Kit

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
92

Bioreactors

BioFlo® 120 Bioreactors

Autoclavable Vessel Bundles - Fermentation
Bundles include: Vessel assembly with Rushton-type impeller and baffle assembly, exhaust condenser, heat blanket or heater base (for water
jacketed vessels), vessel connection kit (provides all the necessary accessories for start-up), motor assembly, pH/DO sensors and cables

Ordering information
Description Order no.
Fermentation Vessel Bundle, for BioFlo® 120, heat blanket, direct-drive
1L B120AVB000
2L B120AVB001
5L B120AVB002
10 L B120AVB003
Fermentation Vessel Bundle, for BioFlo® 120, water-jacketed vessel, direct-drive
1L B120AVB004
2L B120AVB005
5L B120AVB006
10 L B120AVB007

Autoclavable Vessel Bundles - Cell Culture

Bundles include: Vessel assembly with pitched-blade impeller, exhaust condenser, heat blanket or heater base (for water-jacketed vessels),
vessel connection kit (provides all the necessary accessories for start-up), motor assembly, pH/DO sensors and cables

Ordering information
Description Order no.
Cell Culture Vessel Bundle, for BioFlo® 120, heat blanket, magnetic-drive
1L B120AVB008
2L B120AVB009
5L B120AVB010
10 L B120AVB011
Cell Culture Vessel Bundle, for BioFlo® 120, water-jacketed vessel, magnetic-drive
1L B120AVB012
2L B120AVB013
5L B120AVB014
10 L B120AVB015
Cell Culture Vessel Bundle, for BioFlo® 120, heat blanket, direct-drive
1L B120AVB016
2L B120AVB017
5L B120AVB018
10 L B120AVB019
Cell Culture Vessel Bundle, for BioFlo® 120, water-jacketed vessel, direct-drive
1L B120AVB020
2L B120AVB021
5L B120AVB022
10 L B120AVB023

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 93

Bioreactors

BioBLU Single-use Bioreactor Bundles - Fermentation1)

Bundles include: Motor assembly, heat blanket, temperature sensor, pressure relief valve, electric-cooled (Peltier) exhaust condenser
for BioBLU 1f or water-cooled exhaust condensor for BioBLU 3f, pH/DO sensors and cables, vessel stand, sampling syringes, and
tubing.
1)
BioBLU Single-Use Bioreactores sold separately

Ordering information
Description Order no.
BioBLU® Single-Use Bioreactor Bundle, for BioFlo® 120, heat blanket, magnetic-drive
BioBLU® 1f B120SUV000
BioBLU® 3f B120SUV001

BioBLU Single-use Bioreactor Bundles - Cell Culture1)

Bundles include: Motor assembly, heat blanket, temperature sensor, pressure relief valves, Electric-cooled (Peltier) exhaust condenser
(BioBLU 1c only) or electric heater band, pH/DO sensors and cables (Optical pH sensor and OP-76 included in BioBLU 3c Single-Use
Bioreactors and up), vessel stand (BioBLU 1c), sampling syringes, and tubing.
1)
BioBLU vessels sold separately

Ordering information
Description Order no.
BioBLU® Single-Use Bioreactor Bundle, for BioFlo® 120, heat blanket, magnetic-drive
BioBLU® 1c B120SUV003
BioBLU® 3c/5c B120SUV004
BioBLU® 5p B120SUV005
BioBLU® 10c/14c B120SUV006
BioBLU® 50c B120SUV007

BioFlo 110/115 Conversion Bundles

Bundles include Vessel heat blanket or heater base (for water jacketed vessels), Vessel connection kit (provides all the necessary
accessories for start-up), Motor assembly, pH/DO cables1)
1)
pH/DO sensors are not included in conversion kits.

Ordering information
Description Order no.
BioFlo® 120 to BioFlo® 110/115 Bundle, heat blanket, direct-drive
1 L vessel B120110000
2 L vessel B120110001
5 L vessel B120110002
10 L vessel B120110003
BioFlo® 120 to BioFlo® 110/115 Bundle, water jacket, direct-drive
1 L/2 L vessel B120110004
5 L/10 L vessel B120110005
BioFlo® 120 to BioFlo® 110/115 Bundle, heat blanket, magnetic-drive
1 L vessel B120110006
2 L vessel B120110007
5 L vessel B120110008
10 L vessel B120110009
BioFlo® 120 to BioFlo® 110/115 Bundle, water jacket, magnetic-drive
1 L/2 L vessel B120110010
5 L/10 L vessel B120110011

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
94

Bioreactors

BioFlo® 120 Bioreactors

Contents of vessel assembly Fermentation vessel, Fermentation vessel, Cell culture vessel, Cell culture vessel,
heat-blanketed water-jacketed heat-blanketed water-jacketed
Water-jacketed vessel with -  - 

head plate1)
Heat-blanketed vessel with  -  -
head plate (includes heat
blanket)1)
Vessel stand  -  -
Direct drive assembly2)    

Magnetic drive assembly2) - -  

Immersion cooling coil  -  -

Thermowell    

Baffle assembly   - -
Two rushton-type impellers   - -
One pitched-blade impeller - -  

Ring sparger    

Sample tube    

Harvest tube    

Single addition tube and    

adaptor
1)
Heat-blanketed vessel assemblies include the heat blanket. Water-jacketed vessel assemblies do not include the base heater.
2)
Cell culture direct drive or magnetic drive assembly based on selected vessel bundle.
■ = standard

Autoclavable Vessel Assemblies - Fermentation

Ordering information
Description Order no.
Fermentation Vessel, for BioFlo® 120, heat blanket, direct-drive
1L 1390 400 000
2L 1390 400 100
5L 1390 400 200
10 L 1390 400 300
Fermentation Vessel, for BioFlo® 120, water jacket, direct-drive
1L 1390 402 000
2L 1390 402 100
5L 1390 402 200
10 L 1390 402 300

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 95

Bioreactors

Autoclavable Vessel Assemblies - Cell Culture

Ordering information
Description Order no.
Cell Culture Vessel, for BioFlo® 120, water jacket, direct-drive
1L 1390 402 400
2L 1390 402 500
5L 1390 402 600
10 L 1390 402 700
Cell Culture Vessel, for BioFlo® 120, water jacket, magnetic-drive
1L 1390 402 800
2L 1390 402 900
5L 1390 403 000
10 L 1390 403 100
Cell Culture Vessel, for BioFlo® 120, heat blanket, direct-drive
1L 1390 400 400
2L 1390 400 500
5L 1390 400 600
10 L 1390 400 700
Cell Culture Vessel, for BioFlo® 120, heat blanket, magnetic-drive
1L 1390 400 800
2L 1390 400 900
5L 1390 401 000
10 L 1390 401 100

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
96

Bioreactors

BioFlo® 120 Bioreactor Accessories

Ordering information
Description Order no.
Adaptor
6 mm port to 6 mm tube M1273-5054
12 mm port to 6 mm tube M1273-5056
12 mm port to 12 mm tube M1273-5058
12 mm port to tri-port M1273-9961
19 mm port to 1 1/2 in tri-clamp M1287-9545
compression fitting, 12 mm port to 12 mm sensor M1273-5040
compression fitting, 19 mm port to 12 mm sensor M1287-5037
compression fitting, for foam/level sensor and sample tube, 6 mm port to foam/level/sample M1273-5042
19 mm port to 19 mm sensor M1294-9542
for 19 mm port, for Pg 13.5 sensor M1294-9544
Septum Kit, 12 mm port, 10 septa included M1273-3031
Blind Plug
6 mm port M1273-9405
12 mm port M1273-9406
19 mm port M1273-9407

Ordering information
Description Order no.
Tube, barbed, d 6 mm
angled tip, L 76.2 mm M1273-9544
blunt tip, L 110.0 mm M1273-9575
angled tip, L 127.0 mm M1273-9545
blunt tip, L 175.0 mm M1273-9574
blunt tip, L 210.0 mm M1273-9432
Spare Parts Kit
1 L/2 L heat-blanketed vessel M1273-9991
5 L/10 L heat-blanketed vessel M1273-9992
1 L/2 L water-jacketed vessel M1273-9998
5 L/10 L water-jacketed vessel M1273-9999
Spare Parts Kit, head plate port o-rings/washers, all vessel sizes M1369-0122
Foam Trap Kit, 250 mL M1273-9942
Foam/Level Sensor
4 in/100 mm 1390 500 200
7.5 in/190 mm 1390 500 400
14 in/355 mm 1390 500 500
Vessel Connection Kit, for BioFlo® 120 1390 010 000
Syringe Sampling Kit, for BioFlo® 120 1390 991 500
Tubing Kit, for BioFlo® 120, all vessel sizes 1390 910 000
Start-Up Kit, fermentation and cell culture, autoclavable vessels M1369-0300
Autoclave Rack, angled, for BioFlo® 120, for 5 L/10 L vessels M1273-9266

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 97

Bioreactors

Vessel 1L 2L 5L 10 L
Baffles M1273-9263 M1273-9264 M1273-9245 M1273-9265
Sampling assembly1) M1273-9946 M1273-9949 M1273-9953 M1273-9956
Sampling tube M1273-92602) M1273-9198 M1273-9170 M1273-9193
Harvest tube M1273-92602) M1273-9197 M1273-9162 M1273-9510
Cooling ring M1273-92593) M1273-9249 M1273-9247 M1273-9250
Microsparger M1273-5007 M1273-5004 M1273-5005 M1273-5006
(10-15 µm pore size) (heat-blanketed)
M1273-5003
(water-jacketed)
Macrosparger M1273-92593) M1273-9256 M1273-9246 M1273-9251
Thermowell M1273-9200 M1273-9201 M1273-9202 M1273-9203
Glass vessel (heat-blanketed) M1273-9907 M1273-9909 M1273-9916 M1273-9918
Glass vessel (water-jacketed) M1273-9908 M1273-9915 M1273-9917 M1273-9919
Exhaust condenser 1390502000 1390502000 1390502000 1390502100
1)
Sampling assembly with glass bottle system
2)
Sample tube and harvest tube of 1L vessels is part of M1273-9260 (sampling/harvest/addition tube assembly)
3)
Cooling ring and macro sparger of 1L vessels is part of M1273-9259 (sparger/cooling ring assembly)

Impellers Rushton-type impeller Pitched-blade impeller, 45 ° pitch Marine impeller

upflow downflow
Order no. M1273- M1273- M1273- M1273- M1273- M1273- M1230- M1273- M1273-
9291 9292 9293 9206 9207 9290 9212 9901 9902
1 L vessel    

2 L vessel    

5 L vessel    

10 L vessel    

Spinfilter
for suspension cells for microcarriers
Order no. M1273- M1273- M1273- M1273- M1273- M1273- M1273- M1273-
3201 3202 3205 3210 3211 3212 3215 3220
1 L vessel  

2 L vessel  

5 L vessel  

10 L vessel  

■ = standard

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
98

Bioreactors

BioFlo® 320 Bioreactors

Description Product features

BioFlo 320 vessels feature an autoclavable borosilicate glass >> Working volume range between 0.6 – 10.5 L
body and stainless steel head plate. Stainless-steel dished-bottom >> Stainless steel dish bottom and water jacketed vessels
vessels are available for rapid heat transfer as well as traditional >> Interchangeable magnetic and direct overhead drive options
water-jacketed vessels for more gentle temperature control. Each >> Rushton, pitched-blade, marine, spinfilter, cell-lift and packed-bed
vessel type is available in four sizes, with interchangeable overhead impellers available
magnetic and direct drive options. A variety of impellers and
multiple industry standard head plate ports are available for user
flexibility.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 99

Bioreactors

Technical specifications
Vessel Volume 1L 3L 5L 10 L
Volume 2.5 L 5L 7.5 L 14 L
Working volume 0.6 - 1.9 L 1.3 - 3.8 L 1.9 - 5.6 L 3.5 - 10.5 L
Material Borosilicate glass, 316 L stainless steel, EPDM o-rings
Agitation
Drive Direct/magnetic overhead drive
Speed ranges 25 - 1200 rpm (Direct Drive) 25 - 500 rpm (Magnetic Drive)
Impellers 2 x Rushton, 1 x Pitched, 1x Marine, Spin Filter, Cell Lift, or Packed-bed
Gassing
Gas supply Overlay and/or Sparger
Sparger Type Ringsparger or microsparger

Monitoring and Control

Temperature sensor Pt100 RTD
Exhaust condensation Water-cooled
Recommended sensor lengths
pH1) 200 mm 225 mm 325 mm 425 mm
pH (packed-bed)1) 200 mm 200 mm 200 mm 225 mm
DO1) 220 mm 220 mm 320 mm 420 mm
DO (packed-bed)1) 120 mm 120 mm 220 mm 220 mm
Redox1) 200 mm 225 mm 325 mm 425 mm
CO21) 220 mm 220 mm 320 mm 420 mm
Head plate ports
6 mm 1 3 3 3
Pg 13.5 ports 9 10 12 12
19 mm 0 1 1 1
Headplate port
Total 10 14 16 16
Autoclave Dimensions
(with exhaust condensor)
Stainless Steel Dished Bottom
Vessels
Outer diameter (O.D.) 19.9 cm (7.8 in) 22.9 cm (9.0 in) 25.6 cm (10.1 in) 29.3 cm (11.5 in)
Height 51.8 cm (20.4 in) 58 cm (22.8 in) 61.2 cm (24.1 in) 67.9 cm (26.7 in)
Water-Jacketed Vessels
Outer diameter (O.D.) 21.6 cm (8.5 in) 23.1 cm (9.1 in) 27.7 cm (10.9 in) 32.3 cm (12.7 in)
Height 55.4 cm (21.8 in) 61.9 cm (24.4 in) 65.4 cm (25.7 in) 72.9 cm (28.7 in)
1)
May require 12 mm compression fitting (M1287-5030)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
100

Bioreactors

BioFlo® 320 Bioreactors

Contents of vessel kits Stainless-steel dished- Stainless-steel dished- Water-jacketed, direct Water-jacketed,
bottom, direct drive bottom, magnetic drive magnetic drive
drive
Water-jacketed vessel with - -  

head plate
Dished-bottom vessel with head   - -
plate (incl. stainless-steel heat
exchanger)
Direct drive  -  -
Magnetic drive -  - 

Baffles  Sold separately  Sold separately

Exhaust condenser    

Agitation motor Included with vessel bundles, separately offered with configured vessels
■ = standard

Kit contents Direct drive Magnetic drive impeller kits Vessel connection kit
impeller kits
Impeller Rushton (2) or Pitched-blade (1) or -
Pitched-blade(1) or marine (1) or
marine (1) spinfilter with marine (1) or
cell-lift (1) or
basket (1)
Drive shaft Included in vessel kit  -
Sparger with head plate adaptor   -
Thermowell   -
Sample tube with head plate   -
adaptor
Harvest tube with head plate   -
adaptor
Inlet & exhaust line with   

absolute filter
Compression fitting adaptors - -  (2x)
for sensors
Foam/level sensor with adaptor - - 

and cable
Temperature sensor - - 

Tri-port/Single addition tube - - 

and adaptor
Sampling kit - - 

■ = standard

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 101

Bioreactors

Stainless steel dished-bottom vessel bundles include vessel kit with direct-drive bearing housing (baffles included), direct-drive motor,
vessel connection kit, and Rushton impeller kit.

Water-jacketed vessel bundles include vessel kit with magnetic drive bearing housing, magnetic-drive motor for autoclavable vessels, and
vessel connection kit.
NOTE: Impeller kit is not included in water-jacketed vessel bundles.

BioBLU Single-Use Bioreactor bundles include magnetic drive motor for single-use vessels, heat blanket, and connection tubing.

Sensors and sensor cables must be purchased individually.

Ordering information
Description Order no.
Vessel Bundle, for BioFlo® 320, stainless-steel dished-bottom, direct-drive
1L M1379-0300
3L M1379-0301
5L M1379-0302
10 L M1379-0303
Vessel Bundle, for BioFlo® 320, water jacket, magnetic-drive
1L M1379-0310
3L M1379-0311
5L M1379-0312
10 L M1379-0313
Single-Use Bioreactor Bundle, for BioFlo® 320
for BioBLU® 1c M1379-0320
for BioBLU® 1f M1379-0321
for BioBLU® 3c/5c M1379-0322
for BioBLU® 5p M1379-0323
for BioBLU® 10c/14c M1379-0324
for BioBLU® 50c M1379-0325

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
102

Bioreactors

BioFlo® 320 Bioreactors

Validated Vessel Configurations
>> Choose from the options below to configure a validated
autoclavable vessel bundle that meets your process needs
>> All items are available for individual ordering
>> Eight independently controlled process gas supplies
>> Sensors and cables not included, sold separately
>> Addition motor not included, sold separately

Ordering information
Description Order no.
Validation, for BioFlo® 320, autoclavable vessel validation M1379-0103
Vessel, for BioFlo® 320, stainless-steel dished-bottom, direct-drive
1L M1379-1001
3L M1379-1002
5L M1379-1003
10 L M1379-1004
Vessel, for BioFlo® 320, water jacket, direct-drive
1L M1379-1005
3L M1379-1006
5L M1379-1007
10 L M1379-1008
Vessel, for BioFlo® 320, stainless-steel dished-bottom, magnetic-drive
1L M1379-1101
3L M1379-1102
5L M1379-1103
10 L M1379-1104
Vessel, for BioFlo® 320, water jacket, magnetic-drive
1L M1379-1105
3L M1379-1106
5L M1379-1107
10 L M1379-1108
Vessel Connection Kit, fermentation and cell culture, autoclavable vessels, all vessel sizes M1379-0115
Rushton-Type Impeller Kit, direct-drive
for 1 L vessel M1379-1011
for 3 L vessel M1379-1012
for 5 L vessel M1379-1013
for 10 L vessel M1379-1014
Pitched-Blade Impeller Kit, direct-drive
for 1 L vessel M1379-1015
for 3 L vessel M1379-1016
for 5 L vessel M1379-1017
for 10 L vessel M1379-1018

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 103

Bioreactors

Ordering information
Description Order no.
Marine Impeller Kit, direct-drive
for 1 L vessel M1379-1019
for 3 L vessel M1379-1020
for 5 L vessel M1379-1021
for 10 L vessel M1379-1022
Pitched-Blade Impeller Kit, magnetic-drive
for 1 L vessel M1379-5068
for 3 L vessel M1379-5069
for 5 L vessel M1379-5070
for 10 L vessel M1379-5071
Marine Impeller Kit, magnetic-drive
for 1 L vessel M1379-5072
for 3 L vessel M1379-5073
for 5 L vessel M1379-5074
for 10 L vessel M1379-5075
Spinfilter Impeller Kit, for suspension cells (10 µm)
for 1 L vessel M1379-1125
for 3 L vessel M1379-1126
for 5 L vessel M1379-1127
for 10 L vessel M1379-1128
Spinfilter Impeller Kit, for microcarriers (75 µm)
for 1 L vessel M1379-1135
for 3 L vessel M1379-1136
for 5 L vessel M1379-1137
for 10 L vessel M1379-1138
Cell-Lift Impeller Kit, for microcarriers (80 µm), for BioFlo® 320
for 1 L vessel M1379-1110
for 3 L vessel M1379-1111
for 5 L vessel M1379-1112
for 10 L vessel M1379-1113
Packed-Bed Impeller Kit, for BioFlo® 320
for 1 L vessel M1379-1140
for 3 L vessel M1379-1141
for 5 L vessel M1379-1142
for 10 L vessel M1379-1143
Air Wash Kit, required with Cell-Lift impeller kits, for BioFlo® 320 and CelliGen 310, all vessel sizes M1287-1150
Baffles, for BioFlo® 320
for 1 L vessel M1287-9217
for 3 L vessel M1287-9218
for 5 L vessel M1287-9219
for 10 L vessel M1287-9220
Decanter Column
for 1 L vessel M1287-1190
for 3 L vessel M1287-1191
for 5 L vessel M1287-1192
for 10 L vessel M1287-1193
Perfusion Kit, for BioFlo® 320, all vessel sizes M1379-1185

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
104

Bioreactors

BioFlo® 320 Bioreactor Accessories

Rushton impeller Pitched-blade impeller, 45° pitch Marine Impeller
Order M1379- M1379- M1379- M1379- M1287- M1287- M1287- M1379- M1287- M1287- M1287- M1379- M1287-
no. 9295 9296 9297 9298 9227 9228 9229 9230 9230 9950 9952 9954 9954
1L   

vessel
3L   

vessel
5L   

vessel
10 L  
1)

2)

1)

2)

vessel
1)
direct drive only, 2) magnetic drive only, ■ = standard

Ordering information
Description Order no.
Adaptor
compression fitting, 6.35 mm port to 1/4 in tube M1287-5034
compression fitting, 6.35 mm port to foam/level sensor M1294-5022
compression fitting, Pg 13.5 port to 1/4 in tube M1287-5033
compression fitting, Pg 13.5 port to 12 mm sensor M1287-5030
compression fitting, Pg 13.5 port to foam/level sensor M1287-5032
compression fitting, 19 mm port to 12 mm sensor M1287-5037
Pg 13.5 port to tri-port (3 x 3.2 mm) M1287-5035
Pg 13.5 port to 1 1/2 in tri-clamp M1287-9544
19 mm port to 1 1/2 in tri-clamp M1287-9545
19 mm port to 19 mm sensor M1294-9542
Blind Plug
6.35 mm port M1294-9534
Pg 13.5 port M1294-9540
19 mm port M1294-9536
Septum Kit, Pg 13.5 port, 10 septa included M1287-5031
Addition/Gas Overlay Kit, for Pg 13.5 port M1287-5043
Foam/Level Sensor
372 mm M1273-5036
206.4 mm F5-137C
User‘s Kit, fermentation and cell culture, autoclavable vessels, all vessel sizes M1379-0110
Head Plate O-Ring Kit, for BioFlo®/CelliGen 310 and BioFlo® 320 M1379-0121
Tubing Connection Kit, kynar M1379-0122
Teflon Ferrule Kit M1379-0116
Quick Connect Kit, polysulfone M1379-0135
Cable Tie Kit, 300 pieces P0700-8125
Tubing Clamp, plastic, pack of 20 M1379-0155
Needle-Free Sampling Valve, set of 10 M1379-0131
Connector, barbed tube to female Luer, 1/4 in tube, set of 10 M1379-0130
Hose Barb, for sparge gas option/overlay gas option, pack of 4 M1379-0145

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 105

Bioreactors

Ordering information
Description Order no.
Spare Parts Kit, stainless-steel dished-bottom vessel
1L M1287-6020
3L M1287-6021
5L M1287-6022
10 L M1287-6023
Spare Parts Kit, water jacket
1L M1287-6030
3L M1287-6031
5L M1287-6032
10 L M1287-6033
Bearing Housing Cap, pack of 10 M1273-9936
Replacement Screen and O-Ring Kit, for Cell-Lift impeller
1L M1287-9985
3L M1287-9986
5L M1287-9987
10 L M1287-9988
Needle-Free Syringe, pack of 10 M1363-9910
Autoclave Rack, angled, for BioFlo® 320, all vessel sizes XMF-8624
Autoclave Rack, for BioFlo® 320, low-profile, 10 L only M1227-9231

Vessel 1L 3L 5L 10 L
Baffles M1287-9217 M1287-9218 M1287-9219 M1287-9220
Sampling assembly (glass M1287-5042 (all vessel sizes)
bottle)
Sampling adaptor to 15/50 mL M1287-9964 (for sampling assembly M1287-5042 only)
tube
Sampling tube M1287-9486 M1287-9487 M1287-9487 M1287-9489
Harvest tube M1287-9482 M1287-9483 M1287-9484 M1287-9485
Macrosparge M1287-9475 M1287-9476 M1287-9477 M1287-9478
Microsparge M1287-5010 M1287-5011 M1287-5012 M1287-5013
Thermowell M1287-9213 M1287-9214 M1287-9215 M1287-9216
Glass vessel (dished-bottom) M1287-9930 M1287-9931 M1287-9932 M1287-9933
Glass vessel (water-jacketed) M1287-9920 M1287-9921 M1287-9922 M1287-9923
Head plate M1287-6950 M1287-6951 M1287-6952 M1287-6953
Exhaust condenser M1287-5039 M1287-5041 M1287-5041 M1287-5045
Bearing assembly (direct drive) M1379-4031 M1379-4032 M1379-4033 M1379-4034
Direct-drive shaft M1287-9470 M1287-9471 M1287-9472 M1287-9473
Bearing assembly (magnetic M1379-4035 (all vessel sizes)
drive)
Magnetic-drive shaft M1287-5050 M1287-5051 M1287-5052 M1287-5053

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
106

Bioreactors

BioFlo®/CelliGen® Bioreactor Replacement Parts

Replacement Rings and Compression Fitting Ferrules
>> Rings are available in 6 mm, 12 mm, and 19 mm size
>> Ferrules are available in 3/16 inch, 1/4 inch, and 12 mm size
>> Material: Teflon

Minimum order quantities may apply.

BioFlo/CelliGen 510,
BioFlo/CelliGen 115, BioFlo/CelliGen 310, BioFlo 610, BioFlo/
BioFlo 120 BioFlo 320 BioFlo 415 CelliGen Pro
1/4 inch back ferrule - H-1351 - -
1/4 inch front ferrule - H-1350 - -
12 mm back ferrule P0240-3201 P0240-3201 - -
12 mm front ferrule P0240-3200 P0240-3200 - -
3/16 inch back ferrule H-1260 H-1260 H-1260 H-1260
3/16 inch front ferrule H-1259 H-1259 H-1259 H-1259
6 mm ring P0100-9860 - - -
12 mm ring P0100-9870 - - -
19 mm ring P0100-9920 - - -

Replacement Septa
>> Red rubber septum
>> Validatable QMI 7-port and QMI 12-port septum

Minimum order quantities may apply.

BioFlo/CelliGen 510,
BioFlo/CelliGen 115, BioFlo/CelliGen 310, BioFlo 610, BioFlo/
BioFlo 120 BioFlo 320 BioFlo 415 CelliGen Pro
Septum P0280-2690 P0280-2690 P0280-2690 P0280-2690
QMI 7-port - - P0280-0640 P0280-0640
QMI 12-Port - - - P0280-0642
Harvest/Sample tube septum - - P0280-0643 -

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 107

Bioreactors

O-Rings
>> Replacement o-rings are available for current and legacy
Eppendorf bench-scale vessels
>> Material of o-rings: EPDM

Minimum order quantities may apply.

BioFlo/CelliGen 115, BioFlo BioFlo/CelliGen 115, BioFlo BioFlo 310/320 (stainless-steel

120 (heat-blanketed vessels) 120 (water-jacketed vessels) dished-bottom vessels)
6 /6.35 mm P0280-5882 P0280-5882 P0280-5882
6 mm sample/foam port P0280-6662 P0280-6662 -
12 mm P0280-5352 P0280-5352 -
Pg 13.5 - - P0280-5912
19 mm P0280-5962 P0280-5962 P0280-5952
Head plate P0280-7922 (1L) P0280-7922 (1L) P0280-8002 (1L)
P0280-8002 (2L) P0280-8002 (2L) P0280-8082 (3L)
P0280-8122 (5L) P0280-8122 (5L) P0280-8122 (5L)
P0280-8122 (10L) P0280-8122 (10L) P0280-8182 (10L)
Base (plate) - P0280-8622 (1L) P0280-8002 (1L)
P0280-8622 (2L) P0280-8082 (3L)
P0280-8682 (5L) P0280-8122 (5L)
P0280-8682 (10 L) P0280-8182 (10L)

CelliGen 310, BioFlo 320 BioFlo/CelliGen 510, BioFlo 610,

(water-jacketed vessels) BioFlo 415 BioFlo/CelliGen Pro
6 /6.35 mm P0280-5882 P0280-6662 -
6 mm sample/foam port - - -
12 mm - P0280-5952 -
Pg 13.5 P0280-5912 P0280-5912 P0280-5912 (BioFlo/CelliGen 510/BioFlo 610)
19 mm P0280-5952 P0280-5952 -
Head plate P0280-8002 (1L) P0280-8512 (5L) P0280-8222 (BioFlo/CelliGen 510-16L)
P0280-8082 (3L) P0280-8612 (10L) P0280-8742 (BioFlo/CelliGen 510-32L)
P0280-8122 (5L) P0280-8612 (15L) P0280-8742 (BioFlo 610-50L)
P0280-8182 (10L) P0280-8792 (BioFlo 610-100L)
P0280-9385 (BioFlo Pro-60L)
P0280-9365 (BioFlo Pro-120L/CelliGen Pro-60L)
P0280-9375 (BioFlo Pro-240L/BioFlo Pro-2400L/
CelliGen Pro-120L)
P0280-9315 (BioFlo Pro-400L)
P0280-9415 (CelliGen Pro-240L)
P0280-9395 (BioFlo Pro-800L/CelliGen Pro-500L
P0280-9405 (BioFlo Pro-1200L)
Base (plate) P0280-8082 (1L) - -
P0280-8122 (3L)
P0280-8182 (5L)
P0280-8242 (10L)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
108

Software

Much more than just bioprocess control

Eppendorf offers several BioCommand Supervisory Control and Data Acquisition (SCADA)

software packages to meet individual requirements in bioprocess control. The comprehensive DASware

software suite stands for next generation bioprocess management with DASware control 6 as the key to

parallel processing.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 109

>> BioCommand® SCADA Software 112 - 113

>> DASware® control 114 - 115
>> DASware® connect 116
>> DASware® analyze 117
>> DASware® design 118 - 119

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
110

Our Portfolio – Your Choice

BioCommand®

Model BioCommand® SCADA Software DASware® control

Page 112 114

Suitable systems All BioFlo/CelliGen systems DASbox, DASGIP, SciVario twin, BioFlo 120,
BioFlo 320
Process control  

Number of parallel units per Up to 5 DASbox: up to 24

controller DASGIP: up to 16
SciVario twin: up to 8
BioFlo systems: up to 8
Automated data logging  

Data historian  

Remote control and monitoring  

(web browser)
Event logging o 

Online charts/trending  

Analyzer integration o
Integration to third-party 

control systems
Design of Experiments
Configurable database queries 
1)

and recipes
Automated Microsoft® Excel®  

and Adobe® PDF export

Qualification Documents o IQ/OQ package optional
1)
Recipes only

■ = standard, o = optional

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 111

DASware® connect DASware® analyze DASware® design

116 117 118

DASbox, DASGIP, SciVario twin, BioFlo 120, DASbox, DASGIP, SciVario twin, BioFlo 120, DASbox, DASGIP, BioFlo 120, BioFlo 320
BioFlo 320 BioFlo 320

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
112

Software

BioCommand® SCADA Software

BioCommand®

Description Product features

Eppendorf offers three BioCommand software packages to enhance >> BioCommand Track and Trend: this entry-level package has been
your ability to monitor, control, and log data from your fermentation designed specifically for researchers and scientists requiring basic
and cell culture processes through your personal computer (PC). data management and monitoring capabilities. It provides the
These Supervisory Control and Data Acquisition (SCADA) packages ability to trend and control parameter setpoints, establish alarm
provide the tools needed for research, optimization, and if needed, settings, and produce batch records; and is ideal for basic process
the security and audit trails to support your regulatory requirements. management.
All are OPC compatible to enable your fermentor or bioreactor to >> BioCommand Batch Control: this intermediate package includes
„talk“ to any other OPC-compatible device in your lab or production all the capabilities of Track and Trend, plus additional enhanced
facility. control features including a sophisticated programming module,
custom synoptic display window, and equipment lock feature. The
added control features of this software package make it ideal for
optimizing your process.
>> BioCommand Batch Control Plus: our premium package includes
all of the features of the previous two packages, adding three levels
of security, event logs, and audit-trail capabilities to be compatible
with the FDA 21 CFR Part 11 requirements. The Batch Control Plus
package allows the power of the New Brunswick BioCommand
software to be utilized in validated processes.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 113

Software

Batch summary screen displays setpoints, current values, and more; Synoptic screen provides graphical representation of process
custom trend screens allow you to compare and track all of your information.
process data.

Ordering information
Description Order no.
BioCommand® Bioprocessing Software, Track and Trend M1326-0000
BioCommand® Bioprocessing Software, Batch Control M1326-0010
BioCommand® Bioprocessing Software, Batch Control Plus M1326-0020

Accessories
Description Order no.
Cable, for BioCommand®, RS-232-RS-422 M1286-8010
Connection Kit, for BioCommand® M1286-0100
Converter, RS-232 serial pin-out to RS-422 cable connection P0460-7550
Interconnecting Cable, for BioCommand® to controller connection, 50 ft/15.2 m M1171-8010
RS-232 Interface Box, RS-232 to USB, 8 ports M1287-0020
Ethernet Switch, 18 ports P0460-2591

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
114

Software

DASware® control

Description Product features

DASware control is the bioprocess control software at the core of all >> Parallel process control with individual control of each vessel:
DASGIP Parallel Bioreactor Systems and the DASbox Mini Bioreactor DASbox Mini Bioreactor System: up to 24 vessels, DASGIP Parallel
System and offers a parallel process design right from the scratch. Bioreactor Systems: up to 16
Combined with extensive embedded process automation features, >> Seamless integration with SciVario twin and BioFlo systems (up to
intelligent recipe management and integrated report generating 8 controllers)
capabilities it delivers an unprecedented level of integral process >> Integrated batch functionality for process and recipe management
documentation. >> Ideally suited for Design of Experiments (DoE)
The seamless integration of industry-leading OPC communication >> Parallel calibration and cleaning procedures
enables implementation of a host of solutions associated with QbD, >> Customized views and user-defined functions
DoE, PAT and the like. These are offered in the powerful DASware >> Automated data export, reporting and chart creation for Microsoft®
solution suite that integrates with DASware control. Excel®
>> Professional database with managed access (Postgre SQL Server)
>> OPC communication for easy integration with third party-
equipment using DASware connect and DASware analyze
>> Online batch-to-batch comparison, integrated analysis of offline
values, online calculated values, and alarm notification
>> IQ/OQ package available

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 115

Software

The parallel design of DASware control allows for the operation of a In the Process View of DASware control, the user finds all relevant
DASGIP Parallel Bioreactor System with up to 16 vessels. information at a glance and can access and edit individual parameter
settings.

Ordering information
Description Order no.
DASware® control, including PC, OS, and licenses
for 4-fold DASGIP® system 76DGCS4
for 8-fold DASGIP® system 76DGCS8
for 12-fold DASGIP® system 76DGCS12
for 16-fold DASGIP® system 76DGCS16
for 4-fold DASbox® Mini Bioreactor System 76DXCS4
for 8-fold DASbox® Mini Bioreactor System 76DXCS8
for 12-fold DASbox® Mini Bioreactor System 76DXCS12
for 16-fold DASbox® Mini Bioreactor System 76DXCS16
for 20-fold DASbox® Mini Bioreactor System 76DXCS20
for 24-fold DASbox® Mini Bioreactor System 76DXCS24
DASware® control Upgrade, for DASGIP® system, incl. database update and licenses
adding 4 vessels 76DGCS+4
DASware® control Upgrade, for DASbox® Mini Bioreactor System, incl. database update and licenses
adding 4 vessels 76DXCS+4
Software Update, from DASGIP® control 4.X or DASware® control 5.X to DASware® control 6
for 4 vessels, (with Windows®10) 76DWC6UPD
for BioFlo, (with Windows®10) 76DWC6UPDBF
for SciVario twin, (with Windows®10) 76DWC6UPDSVT

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
116

Software

DASware® connect

Description Product features

DASware connect was designed to integrate DASbox and DASGIP >> Integration of bioreactor systems into legacy control systems and
systems, SciVario twin, and BioFlo/CelliGen bioprocess control corporate historians using OPC technology
stations into process control systems and legacy corporate >> Interfacing with scientific software packages like LabVIEW® and
historians. This includes but is not limited to Emerson® DeltaV™, MATLAB®
Siemens® SIMATIC PCS 7®, ABB® 800 xA, OSIsoft® PI System and >> Enables, among others, the integration into: Emerson DeltaV,
MatrikonOPC® Historian. DASware connect facilitates company-wide Siemens SIMATIC PCS 7, ABB 800xA, OSIsoft PI System, Matrikon
access to all relevant bioprocess data like set-points, process values, OPC Historian
feed profiles, calibration and controller parameters as well as events
and alarms.

Ordering information
Description Order no.
DASware® connect, OPC server (OPC DA for ext. PCS), for 1 vessel 76DWCON

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 117

Software

DASware® analyze

Description Product features

DASware analyze enables seamless integration of sampling and >> Integration of third-party lab devices into bioreactor control units
analytical laboratory devices to the bioreactor system. A broad >> Enables bidirectional OPC interconnectivity, process-triggered
and growing range of analyzers can be integrated, among them feedback control loop and sampling on demand
nutrient analyzers, cell counters, biomass monitors, HPLC and mass >> Allows for online calculations and event- or data-driven decisions
spectrometers. The OPC network protocol as well as the Modbus® >> Users benefit from integration of existing benchtop equipment,
protocol allows for interconnectivity between the bioreactor system better process understanding and optimal process control
and the analyzer, including the possibility of direct feedback from the >> Integration includes nutrient analyzers and cell counters, biomass
bioreactor system according to online measured analytical data. This monitors, mass spectrometers, automation platforms and
facilitates feedback control loops for nutrients, biomass or product autosamplers, HPLC, and Raman spectroscopy
concentrations. Online calculations as well as event- and data-driven
decisions are supported. The bidirectional OPC communication,
available for supporting devices enables sampling on demand and
process-dependent analyzer panel selection.

Ordering information
Description Order no.
DASware® analyze, OPC client standard (OPC DA e.g. for external analyzer), for 1 vessel 76DWANA
DASware® analyze, serial/Modbus integration (e.g. for external biomass sensors), license for 1 vessel 76DWANAM
DASware® analyze, cable and license
for 4 Aber® Futura® sensors 76DWANA4AF
for 4 Hamilton® Fogale sensors 76DWANA4HF

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
118

Software

DASware® design

Description Product features

The DASbox Mini Bioreactor System and the DASGIP Parallel >> Built-in full factorial DoE: easy definition of experimental factors
Bioreactor Systems serve as ideal platforms to carry out Design and responses; center points and randomized positioning of runs
of Experiments (DoE) on bioreactors in parallel. DASware design >> Integration of third-party DoE tools
automatically compiles DoE information from DoE software tools into >> Recipe generator supporting multiple system layouts
recipes and feedback response information into DoE and multivariate
analysis and reporting tools.
The software comes with a full factorial DoE builder. Alternatively,
a large variety of DoE designs for screening, process development
and optimization can be automatically imported from third-party
DoE tools. Parallel recipes incorporating the DoE factor variations
(i.e. pH, DO, temperature set-points or feed rates) are automatically
populated. Following our Point-Click-Grow concept they can be
carried out on a set of bioreactors with a single mouse-click.

Ordering information
Description Order no.
DASware® design, DoE and local information management, license for 1 vessel 76DWDOE

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 119

Software

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
120

Monitoring & Control

Flexible solutions for monitoring & control

Eppendorf DASGIP® bioprocess analyzer modules deliver accurate measurement of critical process parameters

allowing real-time monitoring (and control) of pH, dissolved Oxygen, temperature, ORP/redox, level/anti foam,

cell density and exhaust. In addition, the DASGIP line includes variable speed pumps, and TMFC gassing stations.

The DASGIP EasyAccess software package allows the modules to be operated as stand-alone solutions.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 121

>> DASGIP® PHPO for Monitoring of pH, DO, Redox and/or Level 124 - 125
>> DASGIP® GA for Exhaust Analysis 126 - 127
>> DASGIP® OD4 for Optical Density Monitoring 128
>> DASGIP® MP8 and MP4 Multi Pump Modules 129
>> DASGIP® TC4SC4 for Temperature and Agitation Control 130 - 131
>> DASGIP® Bioblock 132
>> DASGIP® Rotameters and Gassing Modules 133
>> DASGIP® MX Modules for TMFC Gas Mixing 134 - 135

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
122

Our Portfolio – Your Choice

Model DASGIP® PHPO DASGIP® GA DASGIP® OD4

Page 124 126 128
Number of parallel bioreactors 4/ 8 4/2/1 4
per module
Operable as stand-alone  

pH monitoring and control 

DO monitoring and control o

Level/foam monitoring and o
control
ORP (redox) monitoring and o
control
Optical density measurement 

Exhaust analysis O2, CO2, OTR, CTR, RQ

Feeding
Gas flow control
Gas mixing
Temperature control
Agitation control
■ = standard, o = optional
OTR = Oxygen transfer rate, CTR = Carbon Dioxide transfer rate, RQ = Respiratory Quotient

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 123

DASGIP® MP DASGIP® TC4SC4 DASGIP® MF4 DASGIP® MX

129 130 133 134
4 4 4 4/1

   

TMFC TMFC
1 gas (e.g. air, N2, O2, CO2) 1/2/4 gas (air, N2, O2, CO2)


Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
124

Monitoring & Control

DASGIP® PHPO for Monitoring of pH, DO,

Redox and/or Level

Description Product features

Eppendorf provides a range of DASGIP bioprocess monitoring >> Parallel monitoring of four or eight pH sensors with temperature
modules delivering precise measurement and real-time control compensation and precise control
of pH, dissolved oxygen (DO), redox potential (ORP) and/or level/ >> PHPO modules feature additional DO control and optional level/
foam. Industry standard sensors can be connected. The monitoring foam control
systems enable parallel monitoring of four or eight pH sensors >> PHPORD module for four vessels features additional ORP control
with temperature compensation. Additionally, up to two Pt100 and optional level/foam control
temperature sensors can be connected and two 0/4-20mA/0-10V >> Easy-to-use one- or two-point calibration procedure for pH, DO
analog inputs provide external signal integration. An easy-to-use and temperature
one- or two-point calibration procedure for pH, DO and temperature
is integrated for use with DASware® control.
The four-channel modules PHPO (configured for pH and DO
control) and PHPORD (pH, DO and ORP) each feature four optional
conductivity-based level inputs. These inputs can be used for level
control during continuous operation or automated antifoam addition.

Applications
>> Parallel monitoring and control of crucial process parameters in
cell culture and microbiology
>> Seamless integration with DASGIP Parallel Bioreactor System

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 125

Monitoring & Control

Technical specifications
Model PH4PO4 PH4PO4L PH8PO8 PH4RD4 PH4PO4RD4L
Dimensions (W × D × H) 300 × 320 × 300 × 320 × 300 × 320 × 300 × 320 × 300 × 320 ×
190 mm / 11.8 × 190 mm / 11.8 × 190 mm / 11.8 × 190 mm / 11.8 × 190 mm / 11.8 ×
15.6 × 7.5 in 15.6 × 7.5 in 15.6 × 7.5 in 15.6 × 7.5 in 15.6 × 7.5 in
Weight 8 kg 8 kg 8.2 kg 8 kg 9.4 kg
Typical power consumption 31 W (230 V) / 31 W (230 V) / 31 W (230 V) / 31 W (230 V) / 31 W (230 V) /
20 W (115 V) 20 W (115 V) 20 W (115 V) 20 W (115 V) 20 W (115 V)
pH measurement
Channels 4 4 8 4 4
Measurement range (depending 0 – 14 0 – 14 0 – 14 0 – 14 0 – 14
on sensor)
DO measurement
Channels 4 4 8 – 4
Measurement range (depending 0 – 500 % DO 0 – 500 % DO 0 – 500 % DO – 0 – 500 % DO
on sensor)
Temperature compensation/measurement
Pt100 inputs 2 2 2 2 2
NTC inputs1) 4 4 8 – 4
Redox/ORP measurement
Channels – – – 4 4
Measurement range (depending – – – -2000 – 2000 mV -2000 – 2000 mV
on sensor)
Level measurement
Channels – 4 – – 4
1)
NTC = Negative Temperature Coefficient

Ordering information
Description Order no.
DASGIP® PH4PO4 Monitoring Module, for 4 vessels, without sensors, pH and DO 76DGPH4PO4
DASGIP® PH4PO4L Monitoring Module, for 4 vessels, without sensors, pH and DO with level/anti foam option 76DGPH4PO4L
DASGIP® PH8PO8 Monitoring Module, for 8 vessels without sensors, pH and DO 76DGPH8PO8
DASGIP® PH4PO4RD4L Monitoring Module, for 4 vessels, without sensors, pH, DO and redox with level/anti foam 76DGPH4PO4RDL
option

Accessories
Description Order no.
DO Cable, for DO sensor, for 1 vessel, T82 connector 76DGPOT82
Foam/Level Cable, for connecting a level sensor to a DASGIP® module, for 1 vessel 76DGLVLC
DO Cable, for DO sensor (optical), for 1 vessel, VP8 connector 76DGPOVP8
pH/Redox Cable, for pH/Redox Sensor, for 1 vessel, AK9 connector 76DGPHRDAK9
Platinum RTD Temperature Sensor, 100 Ohm class A, O.D. 1.6 mm, L 300 mm, cable L 3 m 78103304
i For sensors, see page 152- 168.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
126

Monitoring & Control

DASGIP® GA for Exhaust Analysis

Description Product features

The DASGIP GA4 exhaust analyzer supports precise online measurement >> Online calculation of OTR, CTR and RQ allowing for direct
of exhaust oxygen and carbon dioxide in four discrete analyzer channels. feedback
An integrated mass flow sensor allows online calculation and monitoring >> Available with two alternative electrochemical O2 sensors to best
of oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR) and serve individual customer‘s needs (1 – 50 % O2 or 0 – 100 % O2)
respiratory quotient (RQ), permitting direct conclusions on the metabolic >> Can be operated as a stand-alone solution with EasyAccess
state of the culture and online feedback loops. Optionally the DASGIP GA4 Software
can be equipped with an analog input/output interface for easy integration >> Humidity and temperature compensation (rHT option)
into third party systems.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 127

Monitoring & Control

Technical specifications
Model DASGIP® GA DASGIP® GA4E
Dimensions (W × D × H) 300 × 320 × 190 mm / 11.8 × 15.6 × 7.5 in 300 × 320 × 190 mm / 11.8 × 15.6 × 7.5 in
Weight 12.1 kg 12.1 kg
Typical power consumption 47 W (230 V)/36 W (115 V) 47 W (230 V)/36 W (115 V)
Exhaust oxygen measurement
Channels (O2, CO2, mass flow each) 4 4
Measuring principle Zirconium Dioxide (ZrO2) Galvanic Cell
Measurement range 1 – 50 % 0 – 100 %
Pressure range 0,8 – 1,2 bar 0,8 – 1,2 bar
Channels 4 4
Exhaust carbon dioxide measurement
Measurement range 0 – 25 % 0 – 25 %
Pressure range 0,8 – 1,2 bar 0,8 – 1,2 bar
Mass flow measurement
Measurement range 0 – 300 sL/h 0 – 300 sL/h

Ordering information
Description Order no.
DASGIP® GA4 Exhaust Analyzing Module, including accessories for 4 vessels, including analog I/O option
O2 1 – 50 % and CO2 0 – 25 % 76DGGA4X
O2 0 – 100 % and CO2 0 – 25 % (GA4E) 76DGGA4EX
DASGIP® GA4 Stand-Alone Exhaust Analyzing Module, including analog I/O option, including accessories, without relative humidity
measurement
for 1 vessel, O2 1 – 50 % and CO2 0 – 25 % 76DMGA1X
for 1 vessel, O2 0 – 100 %, CO2 0 – 25 % (GA1E) 76DMGA1EX
for 2 vessels, O2 1 – 50 % and CO2 0 – 25 % 76DMGA2X
for 2 vessels, O2 0 – 100 %, CO2 0 – 25 % (GA2E) 76DMGA2EX
for 4 vessels, O2 1 – 50 % and CO2 0 – 25 % 76DMGA4X
for 4 vessels, O2 0 – 100 %, CO2 0 – 25 % (GA4E) 76DMGA4EX

Accessories
Description Order no.
Kit to Compensate Relative Humidity and Temperature, for DASGIP® GA4
including accessories, for 1 vessel 76DGGA1RHT
including accessories, for 2 vessels 76DGGA2RHT
including accessories, for 4 vessels 76DGGA4RHT

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
128

Monitoring & Control

DASGIP® OD4 for Optical Density Monitoring

Description Product features

The DASGIP OD4 monitoring module is suitable for applications in >> Optical absorbance measurement in 4 bioreactors
cell culture and microbiology enabling parallel optical absorbance >> Runs with industry standard sensors, various sensor sizes available
measurement in 4 bioreactors. Integrated correlations to offline >> Integrated correlation to user-defined offline values
parameters such as OD600 or cell dry weight (CDW) provide online >> Can be operated as a stand-alone solution with EasyAccess
cell growth information. The DASGIP OD4 module can be operated Software
as a stand-alone module or be integrated into legacy control systems
and historians.

Technical specifications
Model DASGIP® OD4
Dimensions (W × D × H) 300 × 320 × 190 mm / 11.8 × 15.6 × 7.5 in
Weight 7.6 kg
Typical power consumption 21 W (230 V)/11 W (115 V)
OD measurement
Channels 4
Measurement range (depending on sensor) 0 – 5 AU

Ordering information
Description Order no.
DASGIP® OD4 Monitoring Module for Optical Density Measurement, for 4 vessels, including transmitter and cables, 76DGOD4
without sensors
DASGIP® OD4 Stand-Alone Monitoring Module for Optical Density Measurement, for 4 vessels, including 76DMOD4
transmitter and cables, without sensors, including DASGIP® EasyAccess software
i For OD sensors, see page 170.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 129

Monitoring & Control

DASGIP® MP8 and MP4 Multi Pump Modules

Description
DASGIP variable speed pump modules MP8 and MP4 provide eight
and four high precision speed controlled miniature peristaltic pumps,
respectively. Pump head tubings with different inner diameters allow
continuous flow rates from 0.3 – 420 mL/h (MP8) and 0.01 – 5 L/h
(MP4). With set points below the minimum continuous flow rate
duty cycling mode is activated automatically. Both modules can be
operated as stand-alone solutions or be integrated into legacy control
systems.

Product features
>> Bidirectional peristaltic pump heads with digitally controlled
variable speed motors
>> Continuous feed rates (depending on tube diameter) of
0.3 – 420 mL/h (MP8) and 0.01 – 5 L/h (MP4)
>> Embedded parallel calibration procedures
>> Can be operated as stand-alone solutions with EasyAccess
Software

Technical specifications
Model MP8 MP4
Dimensions (W × D × H) 300 × 320 × 190 mm / 11.8 × 15.6 × 7.5 in 300 × 320 × 190 mm / 11.8 × 15.6 × 7.5 in
Weight 8.1 kg 10.3 kg
Typical power consumption 8 W (230 V)/5 W (115 V) 11 W (230 V)/8 W (115 V)
Pumps
Quantity 8 4
Variant Pump head with 4 rollers Spring mounted 2-roller rotor
Drive Speed-controlled planetary drive Speed-controlled planetary drive
Operational modes Continuous and dispensing Continuous and dispensing
Tubes
Standard material PTFE PTFE/C-Flex
Inner diameter (flow rates) 0.25 mm (0.3 – 9.5 mL/h) 0.5 mm (0.01 – 0.07 L/h)
0.5 mm (1.3 – 42 mL/h) 0.8 mm (0.02 – 0.22 L/h)
1.0 mm (4.0 – 122 mL/h) 1.6 mm (0.06 – 0.74 L/h)
2.0 mm (13 – 420 mL/h) 2.4 mm (0.13 – 1.57 L/h)
3.2 mm (0.23 – 2.72 L/h)
4.8 mm (0.43 – 5.04 L/h)
Ordering information
Description Order no.
DASGIP® MP4 Multi Peristaltic Pump Module, for 4 feeds, without feed lines and addition bottles 76DGMP4
DASGIP® MP8 Multi Peristaltic Pump Module, for 8 feeds, without feed lines and addition bottles 76DGMP8
DASGIP® MP4 Stand-Alone Multi Peristaltic Pump Module, for 4 feeds, without feed lines and addition bottles, 76DMMP4
including DASGIP® EasyAccess software
DASGIP® MP8 Stand-Alone Multi Peristaltic Pump Module, for 8 feeds, without feed lines and addition bottles, 76DMMP8
including DASGIP® EasyAccess software
DASGIP® MP8 Multi Peristaltic Pump Module, for 8 feeds, without feed lines and addition bottles, including analog 76DGMP8X
I/O option

Accessories
Description Order no.
Package to Support Calibration of DASGIP® MP4/MP8, including balance
for 4 vessels 76DGMPAC4
for 8 vessels 76DGMPAC8
i For balances, see page 147.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
130

Monitoring & Control

DASGIP® TC4SC4 for Temperature and Agitation Control

Description Product features

DASGIP TC4SC4 Modules for Temperature and Agitation Control >> Individual temperature and agitation control for 4 vessels
provide individual stirring speed and temperature control for four >> Powerful stirring up to 1,600 rpm supports high oxygen transfer
bioreactors. Depending on the overhead drive stirring speeds rates in microbial applications
ranging from 30 to 1,600 rpm can be achieved. For temperature >> Gentle cultivation of animal and human cells is achieved with
control the TC4SC4 supplies four electrical outlets to switch cooling continuously adjustable agitation speeds down to 30 rpm
valves. The TC4SC4B module allows a seamless integration with the >> Use of the TC4SC4B module in combination with the DASGIP
compact temperature control system DASGIP Bioblock. Bioblock enables advanced temperature control up to 99 °C
>> Supports freely programmable scripts, trigger automation, user-
defined profiles and DO cascades (integrated in DASGIP Parallel
Bioreactor System)
>> Can be operated as a stand-alone solution with EasyAccess
Software

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 131

Monitoring & Control

Technical specifications
Model TC4SC4B
Dimensions (W × D × H) 300 × 320 × 190 mm / 11.8 × 15.6 × 7.5 in
Weight 9 kg
Temperature control
Set-up DASGIP Bioblock
Typical control range 5 K above cooling agent temperature – 99 °C
(depending on set-up)
Agitation control
Set-up Overhead drives
Typical speed range (depending 30 – 1,250 rpm/
on drive) 100 – 1,600 rpm

Ordering information
Description Order no.
DASGIP® TC4SC4 Temperature and Agitation Control Module, without sensors, for DASGIP® Bioblock and overhead 76DGTC4SC4B
drives (TC4SC4B), for 4 vessels

Accessories
Description Order no.
Overhead Drive, RE30 (magnetic), 30 – 1250 rpm, digitally encoded, for 1 BioBLU® 1 Single-Use Vessel 76DGRE30SU01
Overhead Drive, RE40 (magnetic), 100 – 1600 rpm, digitally encoded, for 1 BioBLU® 1 Single-Use Vessel 76DGRE40SU01
Overhead Drive, RE30, 30 – 1250 rpm, digitally encoded, for 1 vessel 76DGRE30
Overhead Drive, RE40, 100 – 1600 rpm, digitally encoded, for 1 vessel 76DGRE40

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132

Monitoring & Control

DASGIP® Bioblock
Description
The compact DASGIP Bioblock combined with the DASGIP TC4SC4B
Module for Temperature and Agitation Control provides an integrated
solution for accurate and independent temperature control for 4
bioreactors with overhead-driven agitation.
Each well is equipped with an individual electrical heating element
featuring an integrated safety temperature sensor as well as separate
cooling coils, activated by solenoid valves. A wide choice of DASGIP
vessels suitable for the Bioblock is available (working volumes
ranging from 200 mL – 2 L), including single-use vessels BioBLU 1c
and 1f.

Product features
>> Compact solution for 4 vessels with a footprint of 425 x 520 mm
(17 x 20 in)
>> Accurate temperature control up to 99 °C, individually in each well
>> Wide range of Bioblock-suitable glass and single-use vessels for
cell culture and microbiology
>> Vessels can be directly inserted into the Bioblock without any
additional connections

Technical specifications
Model DASGIP® Bioblock
Dimensions (W × D × H) 425 × 520 × 130 mm / 16.7 × 20.4 × 5.1 in
Weight 18 kg
Typical power consumption 309 W/298 W (230 V)/
(incl. DASGIP TC4SC4) 323 W/297 W (115 V)
Suitable working volumes 200 mL – 1.6 L (cell culture)/
200 mL – 1.8 L (microbiology)
Adjustable temperature range 5 K above cooling agent temperature – 99 °C

Ordering information
Description Order no.
DASGIP® Bioblock, 4-position heating/cooling block, max. temp. 99 °C, for 4 vessels 76DGTBLOCK

Accessories
Description Order no.
DASGIP® CWD4 Cooling Water Distribution Unit, including connection cable
for 4 condenser-/ and 4 cooling finger ports (DASGIP® CWD4+4) 76DGCWD44
for 4 condenser ports (DASGIP® CWD4) 76DGCWD4
Accessories
for DASGIP® CWD4+4, for 4-fold system 76DGCWD44UM
for DASGIP® CWD4, for 4-fold system 76DGCWD4UM
Inline Water Filter, for parallel DASGIP® Bioblock or benchtop systems, including accessories for 4-fold or 8-fold 76DGIWF
systems

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Monitoring & Control

DASGIP® Rotameters and Gassing Modules

DASGIP® WRM Rotameter Gassing Station
>> Designed to be mounted to the DASGIP Bioblock
>> Supplying four-channel rotameter gassing
>> Up to 75 sL/h or up to 260 sL/h gas flow rates

Ordering information
Description Order no.
DASGIP® WRM Rotameter Gassing Station, for 4 vessels, rotameter and manual valves
1 – 75 sL/h 76DGWRM
1 – 260 sL/h 76DGWRMH

Accessories
Description Order no.
Stand for Rotameter Gassing Station, for 1 DASGIP® WRM 76DGWRMRX4
Stand for Rotameter Gassing Station, for 2 DASGIP® WRM 76DGWRMRX8

DASGIP® MF4 for TMFC Gas Supply

>> Gassing of one bioreactor with four separate thermal mass flow-
controlled (TMFC) channels
>> Selectable gas types, including air, N2, O2, CO2
>> Individual set-points for each inlet gas
>> Constant flow rates up to 1200 sL/h

Ordering information
Description Order no.
DASGIP® MF4 Gassing Module, for 4 vessels, mass flow controller
1 – 30 sL/h, 1 – 18 sL/h CO2 76DGMF4F030
4 – 120 sL/h, 4 – 72 sL/h CO2 76DGMF4F120
10 – 300 sL/h, 10 – 180 sL/h CO2 76DGMF4F300
20 – 600 sL/h, 20 – 360 sL/h CO2 76DGMF4F600
40 – 1200 sL/h, 40 – 720 sL/h CO2 76DGMF4F1200

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
134

Monitoring & Control

DASGIP® MX Modules for TMFC Gas Mixing

Description Product features

The DASGIP MX4/4 gas mixing system supplies four separate culture >> Thermal mass flow-controlled (TMFC) gassing of one (MX4/1) or
vessels with independent mixtures of air, nitrogen, oxygen and up to 4 (MX4/4) bioreactors
carbon dioxide. Each gas outlet has separate setpoints for flow rate, >> Individual gas mixing from air, N2, O2 and CO2
and concentrations of O2 and CO2. The standard MX4/4 module with >> Gas flow rates ranging from 0.1 – 50 sL/h (MX4/4) to
a maximum gas flow rate of 50 sL/h per outlet covers a wide range of 40 – 1200 sL/h (MX4/1)
microbial and cell culture applications. For applications with a higher >> Can be operated as a stand-alone solution with EasyAccess
gas flow rate demand, the MX4/4H provides up to 250 sL/h per gas Software
outlet.
The DASGIP MX4/1 model suits pilot scale by providing one vessel
with flow rates up to 1200 sL/h. Optional pressure sensors allow safe
operation of glass bioreactors, BioBLU® Single-Use Bioreactors, and
disposable bags.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 135

Monitoring & Control

Technical specifications
Model MX4/4 MX4/4H MX4/1
Dimensions (W × D × H) 300 × 320 × 190 mm / 11.8 × 300 × 320 × 190 mm / 11.8 × 300 × 320 × 190 mm / 11.8 ×
15.6 × 7.5 in 15.6 × 7.5 in 15.6 × 7.5 in
Weight 16 kg 16 kg 10.2 kg
Typical power consumption 100 W (230 V)/ 100 W (230 V)/ 100 W (230 V)/
90 W (115 V) 90 W (115 V) 90 W (115 V)
Gas inlet
Quantity 4 4 4
Gas types Air, O2, CO2, N2 Air, O2, CO2, N2 Air, O2, CO2, N2
Gas outlet
Quantity 4 4 1
Flow rates (CO2) 0.1 – 50 sL/h 0.5 - 250 sL/h 1 – 30 sL/h (CO2:1 - 18 sL/h)
(CO2:0.1 – 40 sL/h) (CO2:0.5 – 150 sL/h) 4 – 120 sL/h (CO2:4 - 72 sL/h)
10 – 300 sL/h (CO2:10 - 180 sL/h)
20 – 600 sL/h (CO2:20 - 360 sL/h)
40 – 1200 sL/h (CO2:40 - 720 sL/h)

Ordering information
Description Order no.
DASGIP® MX4/4 Gas Mixing Module, mass flow controller, 0.1 – 50 sL/h, 0.1 – 40 sL/h CO2
for 4 vessels 76DGMX44
DASGIP® MX4/4 Gas Mixing Module, mass flow controller, 0.5 – 250 sL/h, 0.5 – 150 sL/h CO2
for 4 vessels 76DGMX44H
DASGIP® MX4/1 Gas Mixing Module, for 4 vessels (4x MX4/1), mass flow controller
20 – 600 sL/h 76DGMX41F600
10 – 300 sL/h 76DGMX41F300
4 – 120 sL/h 76DGMX41F120
1 – 30 sL/h 76DGMX41F030
40 – 1200 sL/h 76DGMX41F1200
DASGIP® MX4/4 Stand-Alone Gas Mixing Module, for 4 vessels, mass flow controller
0.1 – 50 sL/h, 0.1 – 40 sL/h CO2, including 2x 30 m gas tube and DASGIP® EasyAccess 76DMMX44
0.5 – 250 sL/h, 0.5 – 150 sL/h CO2 (MX4/4H), including 2x 30 m gas tube and DASGIP® EasyAccess software 76DMMX44H
DASGIP® MX4/1 Stand-Alone Gas Mixing Module, for 1 vessel, mass flow controller
20 – 600 sL/h, including 2x 30 m gas tube and DASGIP® EasyAccess software 76DMMX41F600
10 – 300 sL/h, including 2x 30 m gas tube and DASGIP® EasyAccess software 76DMMX41F300
4 – 120 sL/h, including 2x 30 m gas tube and DASGIP® EasyAccess software 76DMMX41F120
1 – 30 sL/h, including 2x 30 m gas tube and DASGIP® EasyAccess software 76DMMX41F030
40 – 1200 sL/h, including 2x 30 m gas tube and DASGIP® EasyAccess software 76DMMX41F1200

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
136

Accessories

Sensors, chillers, scales, and more

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 137

>> Fibra-Cel® Disks 138 - 139

>> System Accessories 140 - 147
>> DASGIP® EGC for Exhaust Condensation 148
>> DASGIP® Peltier Exhaust Condensers 149
>> DASGIP® Feeding Accessories 150 - 151
>> DO Sensors 152 - 157
>> pH Sensors 158 - 163
>> Redox Sensors 165 - 168
>> Turbidity Sensors 169
>> CO2 Sensors 169
>> Optical Density Sensors 170
>> Level Sensors 171
>> Temperature Sensors 171

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
138

Accessories

Fibra-Cel® Disks

Description Product features

Fibra-Cel is a solid support growth matrix for mammalian, animal, >> Low pressure drop across the bed of Fibra-Cel minimizes the
and insect cells, used predominantly for production of secreted variability and maintains a global viability of cells over the entire
products such as recombinant proteins and viruses. Fibra-Cel enables bed
sustained long-term periods of high-density growth in perfusion, >> High surface-to-volume ratios increases the total biomass that can
without danger of clogging; and eliminates the need for cell filtration be maintained in the bioreactor greatly enhancing production of
to separate cells from the end product. cellular products
>> Entrapped cells are shielded from the turbulence and are less
susceptible to shear forces from impeller blades and sparger gas
Applications bubbles
>> Hybridoma: DA4-4, 123A, 127A, GAMMA, 67-9-B >> Higher mass transfer of nutrients and oxygen versus standard
>> Anchorage-Dependent: 3T3, COS, Human Osteosarcoma, MRC-5, microcarrier systems
BHK, VERO, CHO, rCHO-tPA, rCHO - Hep B Surface Antigen, HEK >> Fibra-Cel is manufactured and tested according to strict quality
293, rHEK 293, rC127 - Hep B Surface Antigen, Normal Human guidelines
Fibroblasts, Stroma, Hepatocytes >> Composed of USP Class VI polypropylene and polyester non-woven
>> Insect: Tn-368, SF9, rSF9, Hi-5 fiber

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 139

Accessories

High-resolution micrograph of a Fibra‑Cel disk indicating the HEK-293 cells grown on Fibra-Cel disk at day 7 of the growth cycle
polyester mesh with polypropylene support

Ordering information
Description Order no.
Fibra-Cel® Disks
50 g M1292-9984
150 g M1292-9992
250 g M1292-9988
1 kg M1292-9974
Custom package sizes available on request.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
140

Accessories

System Accessories
Interface Kit for RS-232 Device
>> The RS-232 Device Interface Kit provides the ability to integrate
up to eight simple RS-232 devices into your fermentation process
to obtain weight measurements, flow rates or other data. Direct
connection to selected controllers, provides direct integration in
the touchscreen control software.
>> Integration into OPC compatible BioCommand® packages provides
the possibility for the development of powerful feed strategies
based on weights or pump flow rates.
>> The kit includes one USB cable to connect to your PC and an eight-
port RS-232 serial box.
>> This kit is designed to communicate with Mettler Toledo® scales
which use SICS level 0 communication protocol.

Ordering information
Description Order no.
RS-232 Interface Box, USB to RS-232, 8 RS-232 ports 1385 504 100

Analog Input/Output Module

>> OPC server interface
>> Communicates with OPC-compatible BioCommand® packages for
total process control (requires a computer with USB connection)
>> User-definable 0 – 5 V or 4 – 20 mA: 3 inputs, 3 outputs
>> 0-5 V: 4 inputs, 4 outputs
>> Connection of external pumps, transmitters for sensors, exhaust
analyzers

Ordering information
Description Order no.
Analog Input/Output Module
100 – 240 V/50/60 Hz M1372-1001

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 141

Accessories

Recirculating Chillers
>> For use with Eppendorf small and bench-scale systems
>> Powerful heat removal
>> Models from different vendors available

Ordering information
Description Order no.
Recirculating Chiller, ThermoFlex® 1400 PD2, 230 V/50 Hz P0620-2798
Recirculating Chiller, ThermoFlex® 1400 PD2, 120 V/60 Hz P0620-2796
Plumbing Package, for ThermoFlex® recirculating chiller P0620-0959
Pressure Regulator, for ThermoFlex® recirculating chiller, 0 – 20 PSIG (0 – 1.4 bar) P0620-2799
Pressure Gauge, for ThermoFlex® recirculating chiller, 0 – 30 PSIG (0 – 2.1 bar) M1287-9918
Chiller, Julabo® SC5000a
208 – 230 V, 60 Hz P0620-3380
400 V, 50 Hz P0620-3381
Connection Kit, for Julabo® SC5000a chiller, for BioFlo®/CelliGen 510 and BioFlo® 610 M1361-0391
Recirculating Chiller, with 600 W, including accessories
115 V/60 Hz 76DGCHILL06U1
230 V/50 Hz 76DGCHILL06U2
Recirculating Chiller, with 1200 W, including accessories
115 V/60 Hz 76DGCHILL12U1
230 V/50 Hz 76DGCHILL12U2

Benchtop Scale
>> Bench-top scales combine a robust design for long life, integrated
display, and the precision needed to meet a variety of needs.
>> Seamless integration with Eppendorf bench-scale controllers
(requires RS-232 device interface kit)
>> Offered in 6, 15, and 30 kg capacities
>> Benchtop Scale Kits include a connection cable to RS-232.

Ordering information
Description Order no.
Benchtop Scale Kit, Mettler Toledo® ICS425
6 kg, North American plug M1425-1001
15 kg, North American plug M1425-1002
35 kg, North American plug M1425-1003
6 kg, Schuko plug M1425-1004
15 kg, Schuko plug M1425-1005
35 kg, Schuko plug M1425-1006

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
142

Accessories

System Accessories
Variable Speed Pumps
>> Watson-Marlow® variable speed pumps

Ordering information
Description Order no.
Variable-Speed Pump, Watson-Marlow® 120U, with 114DV pump head, 0 – 200 rpm, including connection cable with M1287-9959
free leads
Variable-Speed Pump, Watson-Marlow® 120U, with 114DV pump head, 0 – 200 rpm, including connection cable with M1379-9959
Lumberg® connector, for BioFlo® 320 and BioFlo® 720
Variable-Speed Pump, Watson-Marlow® 120U, with 114DV pump head, 0 – 200 rpm, including connection cable with M1287-9978
Lumberg® connector, for BioFlo®/CelliGen 510 - Allen-Bradley®

Regulator/pre-filter kits
>> Regulator/pre-filter kits for water, gas, and steam
>> Utility connection kit for metric conversions and sterilize-in-place
systems

BioFlo/
CelliGen 115,
System DASbox® DASGIP® BioFlo 120 310 320 415
Single Gas (up to 4 systems) M1273-5002 M1273-5002 M1273-5002 M1273-5002
4-Gas M1363-5002 M1363-5002 M1363-5002
1/4 inch to 6 mm converter kit 1) 77105038 77105038 77105038

Single Water 76DGIWF 76DGIWF

Water (up to 4) M1273-5001 M1273-5001 M1273-5001 M1273-5001
Water (up to 4) - metric 77105037 77105037 77105037
1)
Requires M1363-5002

BioFlo/ BioFlo/
BioFlo/ CelliGen Pro CelliGen Pro BioFlo Pro BioFlo Pro
System CelliGen 510 BioFlo 610 (60 - 120L) (240 - 500 L) (800 - 1200 L) (2400 L)
Water M1361-1100 M1361-1100 M1290-0650 M1290-0654 M1290-0656 M1290-0657
Main Steam M1361-1101 M1361-1101 M1290-0660 M1290-0664 M1290-0666 M1290-0667
Process Steam M1361-1102 M1361-1102 M1290-0670 M1290-0674 M1290-0676 M1290-0677
Process Air M1361-1103 M1361-1103 - - - -
Instrument Air M1361-1104 M1361-1104 M1290-0760 M1290-0760 M1290-0760 M1290-0760
Process Steam to Utility Steam M1361-1105 M1361-1105 - - - -
2)

Utility Connection Kit M1361-9992 M1362-9992 M1298-9992 M1298-9992 M1290-9992

2)
Requires M1361-1101

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 143

Accessories

Tubing
>> Silicone tubing for liquid addition and gassing
>> Polyurethane tubing for gas connections to control station

Ordering information
Description Order no.
Tubing, silicone
I.D. 1/16 in/1.6 mm, O.D. 3/16 in/4.7 mm, L 50 ft/15.2 m M0740-2396
I.D. 1/8 in/3.2 mm, O.D. 1/4 in/6.4 mm, L 25 ft/7.6 m M0740-2445
I.D. 3/16 in/4.7 mm, O.D. 5/16 in/7.9 mm, L 25 ft/7.6 m M0740-2505
I.D. 3/32 in/2.4 mm, O.D. 1/4 in/6.4 mm, L 50 ft/15.2 m M0740-2430
I.D. 1/4 in/6.4 mm, O.D. 3/8 in/9.5 mm, L 25 ft/7.6 m M0740-2542
I.D. 5/16 in/7.9 mm, O.D. 7/16 in/11.1 mm, L 50 ft/15.2 m M0740-2590
Tubing, polyurethane, blue, I.D. 5/64 in/2.0 mm, O.D. 3/16 in/4.7 mm, L 50 ft/15.2 m M0740-3110
Tubing, polyurethane, blue, I.D. 1/8 in/3.2 mm, O.D. 1/4 in/6.4 mm, L 50 ft/15.2 m M0740-3111C3
Tubing, polyurethane, blue, I.D. 5/32 in/4.0 mm, O.D. 1/4 in/6.4 mm, L 25 ft/7.6 m M0740-3113C3

Replacement filters
>> Round and Cartridge Filters
>> Available in 0.2 µm and 1.2 µm

BioFlo/ BioFlo/ BioFlo/

CelliGen BioFlo CelliGen BioFlo BioFlo CelliGen BioFlo
115 120 310 320 415 510 610 BioFlo/CelliGen Pro
Inlet 0.2 Round P0200- P0200- P0200- P0200- - - - -
µm 0491 0495 0491 0495
(Small) (Small)
P0200- P0200-
0495 0495
(Large) (Large)
Inlet 0.2 Cartridge - - - - P0200- P0200- P0200- P0200-4061
µm 0129 4148 4148 (BioFlo Pro 60-120L/CelliGen Pro)
P0200-4060 (BioFlo Pro 240-400L)
P0200-4064 (BioFlo Pro 800-2400L)
Exhaust 0.2 Round P0200- P0200- P0200- - - - - -
µm 0495 0495 0495
Exhaust 0.2 Cartridge - - - P0200- P0200- P0200- - P0200-4061
µm 4130 0129 4147 (BioFlo Pro 60-120L/CelliGen Pro)
P0200-4060 (BioFlo Pro 240-400L)
P0200-4063 (BioFlo Pro 800-1200L)
P0200-4064 (BioFlo Pro 2400L)
Exhaust 1.2 Cartridge - - - - P0200- P0200- P0200- -
µm 4150 4146 4190
Sample 0.2 Round P0200- - P0200- - P0200- - - -
µm 0970 0970 0970

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
144

Accessories

System Accessories
Addition/Harvest Bottles
>> Autoclavable addition/harvest bottles in various sizes
>> Ranging from 250 mL – 10 L

Ordering information
Description Order no.
Foam Trap Kit, 250 mL M1273-9942
Addition/Harvest Bottle Kit, for aerobic processes
250 mL M1362-9905
500 mL M1362-9906
1L M1362-9901
2L M1362-9902
5L M1362-9903
10 L M1362-9904
Addition/Harvest Bottle Kit, for anaerobic processes
250 mL M1362-9913
500 mL M1362-9914
1L M1362-9915
2L M1362-9916
5L M1362-9917
10 L M1362-9918
Addition/Harvest Bottle Kit, for aerobic processes, including bottle holder
250 mL M1273-9989
500 mL M1273-9990
Bottle Holder Kit
for 250/500 mL bottle M1273-9940

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 145

Accessories

Other Accessories
Ordering information
Description Order no.
Hypodermic Needle
8 GA, L 107.95 mm, d 3.26 mm, 12 needles P0440-0061A
18 GA, L 38 mm, pack of 100 P0440-0064
Sample Vials, autoclavable, case of 72
25 mL, with caps M1227-9935
40 mL, with caps P0640-0500
Bearing Housing Cap, pack of 10 M1273-9936
Addition Vessel Kit
4L M1290-0550
7.5 L M1290-0551
11 L M1290-0552

Bioprocess Accessories and Parts

>> Tubing connectors
>> Tri-clamp to barbed tube connectors
>> Luer connectors

Straight connectors
1/4 in to 1/8 in P0242-0460
1/4 in to 1/4 in P0242-0510
1/4 in to 3/16 in P0242-0200
5/16 in to 1/4 in P0242-0201
3/8 in to 1/4 in P0242-0202
T-connector
1/4 x 1/4 x 1/4 in P0242-0210
Quick Connects
Female P0240-2680
Male P0240-2670
Tri-clamp to barbed tube
0.75 inch to 1/8 in barbed tube P0240-0774C3
0.75 inch to 1/4 in barbed tube P0240-0773C3
0.75 inch to 1/2 in barbed tube P0240-0775C3
Tubing Clamps
Clamp, plastic P0160-4460
Clamp, steel P0160-4830
Luer
Barbed tube to male luer, 1/8 in tube, stainless steel P0240-5000
Barbed tube to female luer, 1/4 in tube, set of 10 M1379-0130
Connector, barbed tube to female Luer, 1/8 in tube, polypropylene P0242-0590
Connector, barbed tube to male Luer, 1/8 in tube, polypropylene P0242-0591

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
146

Accessories

System Accessories
DASGIP® Process Computer
>> Intel Core® i5
>> SSD ≥ 240 GB
>> 22 in LCD monitor
>> Microsoft® Windows® 10

Ordering information
Description Order no.
Process Computer for Small Scale Systems, including OS software, DASware® control without licenses, and PC 76DGPCS
hardware, English language, with accessories

Uninterruptible Power Supply for

Bioreactor/Fermenter Systems
>> Uninterruptable power supplies and replacement batteries

Ordering information
Description Order no.
Uninterruptible Power Supply, for DASGIP® system, 230 V/50 Hz, Eaton® 5P 1550i, 1550 VA 78535272
Uninterruptible Power Supply, APC BackUPS BR1500G, 115 V, 60 Hz, 1500 W 78535267
Replacement Battery, for UPS BR1500(I/LCD), RBC33 78110007

DASGIP® CWD Cooling Water Distribution Unit

>> Exhaust cooling and/or temperature control in up to four vessels
>> Available with four or eight ports for condensers and/or cooling
fingers

Ordering information
Description Order no.
DASGIP® CWD4 Cooling Water Distribution Unit, including connection cable
for 4 condenser ports (DASGIP® CWD4) 76DGCWD4
for 4 condenser-/ and 4 cooling finger ports (DASGIP® CWD4+4) 76DGCWD44
Accessories, for DASGIP® CWD4+4, for 4-fold system 76DGCWD44UM
Accessories, for DASGIP® CWD4, for 4-fold system 76DGCWD4UM
Inline Water Filter, for parallel DASGIP® Bioblock or benchtop systems, including accessories for 4-fold or 8-fold systems 76DGIWF

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 147

Accessories

Balance
>> Precision balances e.g. for integration with DASGIP MP8 and MP4
multi pumps
>> Mettler Toledo® MS
>> Available with max. capacities of 3.2 – 12.2 kg

Ordering information
Description Order no.
Precision Balance, including license and cable
3.2 kg 76DGBAL32
4.2 kg 76DGBAL42
6.2 kg 76DGBAL62
8.2 kg 76DGBAL82
12.2 kg 76DGBAL122

Serial Device Servers for DASGIP® Products

>> Connects up to eight serial devices to the Ethernet

Ordering information
Description Order no.
Serial Device Server, with 2 ports
115 V 76DGRSPORT2U1
230 V 76DGRSPORT2U2
Serial Device Server, with 8 ports 76DGRSPORT8

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
148

Accessories

DASGIP® EGC for Exhaust Condensation

Description Product features

The Eppendorf DASGIP EGC4 Module in combination with >> Effective liquid-free exhaust condensation via Peltier technology
our Peltier Exhaust Condensers provides liquid-free exhaust >> Up to four exhaust condensers can be connected
condensation for up to four vessels. Proven effective for the DASbox >> Suitable for BioBLU Single-Use Bioreactors 1c, 1f, 3c, 5c, and 5p
Mini Bioreactor and for the mini scale BioBLU 0.3 Single-Use
Bioreactors, this innovative technology can also be utilized with the
larger vessels of the Eppendorf BioBLU family, BioBLU 1c, 1f, 3c,
5c, and 5p. Optimum recovery of condensate prevents volume loss
due to evaporation and associated changes in osmolarity as well as
blocking of exhaust filters. No cooling agent or chiller is needed so
users benefit from easy handling.

Ordering information
Description Order no.
DASGIP® EGC4 Exhaust Gas Condenser Controller, for 4 Peltier actuators, 110 – 240 V/50/60 Hz 76DGEGC4

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 149

Accessories

DASGIP® Peltier Exhaust Condensers

Description Product features

Our innovative Peltier Exhaust Condenser offers highly effective >> Liquid-free exhaust condensation through Peltier-based cooling
condensation - without the need for a cooling agent or chiller. >> Highly effective condensation minimizes volume loss caused by
Volume loss due to evaporation is thereby minimized and blocking evaporation
of exhaust filter prevented. The condenser‘s automatic slide in >> Prevents blocking of exhaust filter
activation and slide out deactivation mode satisfies users with its >> Automatic activation/deactivation with proximity sensor
easy handling. >> Automatic and manual de-icing functionality
The Peltier-based exhaust condenser was designed for use with the >> Suitable for BioBLU Single-Use Bioreactors 0.3c, 0.3f, 1c, 1f, 3c,
Eppendorf DASbox Mini Bioreactor System and is ready-to-use with 5c, and 5p
both autoclavable (DASbox Mini Bioreactor) and BioBLU Single-Use
Bioreactors in combination with the DASGIP EGC4 module. Applications
>> Cell culture and fermentation in mini scale using the Eppendorf
DASbox
>> Small and bench scale applications with BioBLU Single-Use
Bioreactors

Ordering information
Description Order no.
Actuator Unit for Peltier Exhaust Condenser
including cable L 1.6 m 78201321
including cable L 4.0 m 78201330
Exhaust Condenser Adaptor, including insulation
for DASbox® Mini Bioreactors autoclavable vessels 78201323
for BioBLU® 0.3c/0.3f/1c Single-Use Bioreactors 78201322
for BioBLU® 1f Single-Use Bioreactors 78109131
for BioBLU® 3c/5c/5p Single-Use Bioreactors 78201326
Exhaust Condenser Unit, for SciVario® twin, Peltier based 7600 241 001

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
150

Accessories

DASGIP® Feeding Accessories

DASGIP® Pump Head Tubings
>> Available with different inner diameter (ID) and wall thickness (W)
>> Material: Bioprene®/Marprene®
>> Connectors: male/female, female/female
>> Suitable for DASGIP MP4 and MP8, respectively

Ordering information
Description Order no.
Pump Head Tubing, for DASGIP® MP8 pump, PharMed®
I.D. 0.25/W 0.85 mm, male/female 78510198
I.D. 0.25/W 0.85 mm, female/female 78510119
Pump Head Tubing, for DASGIP® MP4 pump, Bioprene®
I.D. 0.5/W 1.6 mm, male/female 78510251
I.D. 2.4/W 1.6 mm, male/female 78510254
I.D. 3.2/W 1.6 mm, male/female 78510255
Pump Head Tubing, for DASGIP® MP8 pump, Bioprene®
I.D. 0.5/W 1.05 mm, male/female 78510117
I.D. 0.5/W 1.05 mm, female/female 78510118
I.D. 1.0/W 1.05 mm, male/female 78510109
I.D. 1.0/W 1.05 mm, female/female 78510236
Pump Head Tubing, for DASGIP® MP8 pump, Peripren®
I.D. 2.0/W 0.8 mm, male/female 78510197
I.D. 2.0/W 0.8 mm, female/female 78510237
Pump Head Tubing, for DASGIP® MP4 pump, Marprene®
I.D. 0.5/W 1.6 mm, female/female 78510292
I.D. 0.8/W 1.6 mm, female/female 78510293
I.D. 1.6/W 1.6 mm, female/female 78510295
I.D. 2.4/W 1.6 mm, female/female 78510296
I.D. 3.2/W 1.6 mm, female/female 78510297
I.D. 4.8/W 1.6 mm, female/female 78510298

DASGIP® Head Gears

>> Allows easy transfer of liquids from addition bottles to the
bioreactor
>> For GL45 neck

Ordering information
Description Order no.
Head Gear, for addition bottles with GL45 neck, C-Flex®
female Luer lock 78510311

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 151

Accessories

DASGIP® Feed Lines

>> Available with different inner diameter (ID) and length (L)
>> Material: C-Flex® or PTFE
>> Connectors: male/male

Ordering information
Description Order no.
Feed Line, uncolored with 2x Luer lock fittings, I.D. 0.8 mm, PTFE
L 1 m, male/male 78510241
L 3 m, male/male 78510243
Feed Line, with 2x Luer lock fittings, male/male, C-Flex®
I.D. 0.8 mm, L 1 m 78510309
I.D. 0.8 mm, L 2 m 78510310
I.D. 3.2 mm, L 1 m 78510320
I.D. 3.2 mm, L 2 m 78510321
Feed Line Set, for SciVario® twin double pump drawer, PTFE
I.D. 0.25 mm 7600 252 001
I.D. 0.5 mm 7600 252 002
I.D. 1.0 mm 7600 252 003
I.D. 2.0 mm 7600 252 004
Feed Line Set, for SciVario® twin double pump drawer, C-Flex®
I. D. 0.25 mm 7600 252 011
I. D. 0.5 mm 7600 252 012
I. D. 1.0 mm 7600 252 013
I. D. 2.0 mm 7600 252 014
Feed Line Set, for SciVario® twin single pump drawer, C-Flex®,
I. D. 0.5 mm 7600 252 101
I. D. 0.8 mm 7600 252 102
I. D. 1.6 mm 7600 252 103
I. D. 2.4 mm 7600 252 104
I. D. 3.2 mm 7600 252 105
I. D. 4.8 mm 7600 252 106

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
152

Accessories

DO Sensors
DO Sensors
>> Accurate monitoring of dissolved oxygen
>> Standard clark electrodes as well as optical sensors
>> Various sensor lengths available

Model
Vessel Size Sensor "DASbox® Spinner Spinner 1 L/ Spinner Bioreactor Bioreactor Photo­
Group 0.25 L" 0.7 L/ Stirrer 1.5 L 1.5 L/ 2.5 L 3.5 L Bioreactor
Stirrer 1 L Stirrer 1.8 L 1.0 L /2.5 L
DASbox® A, D, F 120 mm - - - - - -
MiniBioreactor
DASGIP® Bioblock A, D, F - 220-225 mm 220-225 mm 320-325 mm - - -
DASGIP® Benchtop A, D, F - - - - 220-225 mm 320-325 mm -
DASGIP® A, D, F - - - - - - 220-225 mm
PhotoBioreactors
SciVario® twin A, B, C, D, - 220-225 mm 220-225 mm 320-325 mm 220-225 mm 320-325 mm -
E, F

Model
Vessel Size Sensor Group BioBLU® BioBLU® 1c/f BioBLU® BioBLU® 5p BioBLU® BioBLU® 50c
0.3c/f/sc 3c/5c/3f 10c/14c
DASbox® A, D, F, G 162 mm - - - - -
MiniBioreactor
DASGIP® Bioblock A, D, F, G - 278 mm - - - -
DASGIP® Benchtop A, D, F, G - 278 mm 220-225 mm - - -
SciVario® twin A, B, C, D, E, - 278 mm 220-225 mm - 355 mm 526 mm
F, G

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 153

Accessories

Ordering information
Sensor
Description Group Order no.
DO Sensor, Mettler Toledo® InPro 6820, autoclavable, O.D. 12 mm A
L 120 mm 78108018
L 220 mm 78108026
L 320 mm 78108022
L 355 mm 78108076
L 526 mm 78108077
DO Sensor, Mettler Toledo® InPro 6850i, ISM®, autoclavable, O.D. 12 mm B
L 220 mm 78108063
L 320 mm 78108067
DO Sensor (Optical), Mettler Toledo® InPro 6860i, ISM®, autoclavable, O.D. 12 mm C
L 220 mm 78108071
L 320 mm 78108072
DO Sensor, Hamilton® OxyFerm, autoclavable, O.D. 12 mm D
L 120 mm 78108023
L 225 mm 78108039
L 325 mm 78108040
DO Sensor, Hamilton® OxyFerm FDA ARC, autoclavable, O.D. 12 mm E
L 225 mm 78108064
L 325 mm 78108069
DO Sensor (Optical), Hamilton® VisiFerm™, autoclavable, O.D. 12 mm, H0 cap F
L 120 mm 78108058
L 215 mm 78108059
L 325 mm 78108060
DASGIP® DO Sensor O.D. 4.7 mm G
including cable L 3 m and storage chamber for BioBLU® 0.3, L 162 mm 78108046
including cable L 1 m and storage chamber for BioBLU® 1, L 278 mm, for SciVario® twin 78108065
including cable L 3 m and storage chamber for BioBLU® 1, L 278 mm 78108051

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
154

Accessories

DO Sensors
Model
Vessel Size Sensor Group BioBLU® BioBLU® 1c/f BioBLU® BioBLU® 5p BioBLU® BioBLU® 50c
0.3c/f/sc 3c/5c/3f 10c/14c
BioFlo®/CelliGen 115 H - - 220-225 mm 120 mm 355/420 mm 526 mm
BioFlo® 120 G, H, I, J - 278 mm 220-225 mm 120 mm 355/420 mm 526 mm
BioFlo®/CelliGen 310 H - - 220-225 mm 120 mm 355/420 mm 526 mm
BioFlo® 320 G, H, I, J - 278 mm 220-225 mm 120 mm 355/420 mm 526 mm
CelliGen BLU - H - - 220-225 mm 120 mm 355/420 mm 526 mm
1st generation
CelliGen BLU - H - - 220-225 mm 120 mm 355/420 mm 526 mm
2nd generation

Model
Vessel Size Sensor 1L 2L 3L 5L 10 L 15 L All Vessels
Group
BioFlo®/CelliGen 115 H 160 mm 220 mm - 320 mm 420 mm - -
BioFlo® 120 H, I, J 160 mm 220 mm - 320 mm 420 mm - -
BioFlo®/CelliGen 310 H 220 mm - 220 mm 320 mm 420 mm - -
BioFlo® 320 H, I, J 220 mm - 220 mm 320 mm 420 mm - -
CelliGen 310 H 120 mm - 120 mm 220 mm 220 mm - -
(packed-bed)
BioFlo® 320 H, I, J 120 mm - 120 mm 220 mm 220 mm - -
(packed-bed)
BioFlo® 415 H - - - 320 mm 420 mm 625 mm -
BioFlo®/CelliGen 510 H, I, J * - - - - - - 120 mm
BioFlo® 610 H, I, J * - - - - - - 120 mm
BioFlo® 720 H, I, J 225 mm
BioFlo®/CelliGen Pro H, I, J * - - - - - - Standard
Housing -
120 mm
Retractable
Housing -
320 mm
* Depending on option installed

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 155

Accessories

Ordering information
Sensor
Description Group Order no.
DASGIP® DO Sensor O.D. 4.7 mm G
including cable L 1 m and storage chamber for BioBLU® 1, L 278 mm, for SciVario® twin 78108065
including cable L 3 m and storage chamber for BioBLU® 1, L 278 mm 78108051
DO Sensor, Mettler Toledo® InPro 6810, 160 mm, angled VP connector H P0720-6281
DO Sensor, Mettler Toledo® InPro 6820, straight T-82 connector
L 225 mm P0720-6526
L 355 mm P0720-6525
L 526 mm P0720-6529
DO Sensor, Mettler Toledo® InPro 6830, angled T-82 connector
L 120 mm P0720-6280
L 160 mm P0720-6580
L 220 mm P0720-6282
L 320 mm P0720-6283
L 420 mm P0720-6284
DO Sensor, Hamilton® OxyFerm FDA, 625 mm, straight T-82 connector P0720-6520
DO Sensor, Mettler Toledo® InPro 6850i, ISM® I
L 120 mm P0720-6652
L 220 mm P0720-6653
L 320 mm P0720-6654
L 420 mm P0720-6655
DO Sensor (Optical), Mettler Toledo® InPro 6860i, ISM® J
L 120 mm P0720-6651
L 220 mm P0720-6660
L 320 mm P0720-6661
L 420 mm P0720-6662
L 590 mm P0720-6666

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
156

Accessories

DO Sensors
Adaptor/
sensor
Model Cable housing
Analog Digital Digital
VP8 (ISM-
Optical DO/ Head plate Side-Wall Sensor
T-82 AK9 (ISM) ARC) adaptor Adaptor Housing
DASbox® MiniBioreactor 78522099 – 78522042 – – –
DASGIP® Parallel Bioreactor 78522099 – 78522042 771020161) – –
System
SciVario® twin 7600222501 7600220001 7600220002 771020161) – –
BioFlo®/CelliGen 115 P0720-2336 – – M1273-5040 – –
BioFlo® 120 1390810600 M1379-8108 M1379-8107 M1273-5040 – –
BioFlo®/CelliGen 310 P0720-2333 – – M1287-5030 – –
BioFlo® 320 M1379-8106 M1379-8108 M1379-8107 M1287-5030 – –
BioFlo® 415 P0720-2333 – – – – –
CelliGen BLU - 1st Generation P0720-2336 – – – – –
CelliGen BLU - 2nd Generation P0720-2336 – – – – –
BioFlo®/CelliGen 510 P0720-2336 – – – M1361-9208 P0720-
6240C3
BioFlo® 610 P0720-2336 – – – M1361-9208 P0720-
6240C3
BioFlo® 720 1385810600 1385810700 1385810800 – 0045890038 –
BioFlo®/CelliGen Pro - Standard P0720-2342 – – – – P0720-
Housing 6450C1
BioFlo®/CelliGen Pro - P0720-2342 – – – – P0720-5570C
Retractable Housing
1)
For DASGIP / SciVario twin Benchtop Biore-
actors

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 157

Accessories

Ordering information
Description Order no.
Sensor Cable, for connecting DO sensors to DASGIP® modules, grey, with plug type T82, L 2.9 m (9.5 ft) 78522099
Sensor Cable, for connecting DO sensors to DASGIP® modules, grey, with plug type VP8 for VisiFerm™, L 3 m (9.8 ft) 78522042
Sensor Cable, for analog DO sensors, for SciVario® twin, with plug type T82 7600 222 501
Sensor Cable, for ISM® sensors, for SciVario® twin, with plug type AK9 7600 220 001
Sensor Cable, for ARC® sensors, for SciVario® twin, with plug type VP8 7600 220 002
Sensor Cable, for analog DO sensors, for BioFlo® 120, with plug type T82, L 1 m (3.3 ft) 1390 810 600
Sensor Cable, for analog DO sensors, for BioFlo® 320, with plug type T82, L 1 m (3.3 ft) M1379-8106
Sensor Cable, for optical DO sensors, ISM®, with plug type VP8, L 1 m (3.3 ft) M1379-8107
Sensor Cable, for ISM® sensors, with plug type AK9, L 1 m (3.3 ft) M1379-8108
Sensor Cable, for analog DO sensors, for BioFlo®/CelliGen 310, BioFlo® 410, BioFlo® 415, with plug type T82, L 1.1 m P0720-2333
(3.5 ft)
Sensor Cable, for analog DO sensors, for BioFlo®/CelliGen 115, BioFlo®/CelliGen 510, BioFlo® 610, CelliGen BLU (2nd P0720-2336
generation), with plug type T82, L 1.4 m (4.6 ft)
Sensor Cable, for analog DO sensors, for BioFlo®/CelliGen Pro, with plug type T82, L 1.8 m (6 ft) P0720-2342
Sensor Cable, for analog DO sensors, controller with Lumberg® connector, with plug type T82, L 3 m (9.8 ft) 1385 810 600
Sensor Cable, for optical DO sensors, controller with Lumberg® connector, with plug type VP8, L 3 m (9.8 ft) 1385 810 700
Sensor Cable, for ISM® sensors, controller with Lumberg® connector, with plug type AK9, L 3 m (9.8 ft) 1385 810 800
Sensor Housing, stainless steel
for 25 mm Ingold® port, for 120 mm sensor length, with material certificate P0720-6450C1
InFit® 761, for 25 mm Ingold® port, for 120 mm sensor length, with material certificate P0720-6240C3
retractable, InTrac® 797, for 25 mm Ingold® port, 325 mm sensor length, with material certificate P0720-5570C
Ingold® Port Weldment, converts 1 1/2 in sanitary to 25 mm Ingold® port M1361-9208
Sensor Sleeve Bellow, for attaching sensor to SUB, with AseptiQuik® connector 0045 890 038
Adaptor
VP connector to T-82 connector, for analog sensors P0720-6470
Pg 13.5 female thread to M18x1.5 male thread 77102016
compression fitting, 12 mm port to 12 mm sensor M1273-5040
compression fitting, Pg 13.5 port to 12 mm sensor M1287-5030
Power Adaptor, for optical DO sensors, VP to T-82 connector P0720-9771

Accessories
Description Order no.
DO Electrolyte, Mettler Toledo®, pack of 3 x 25 mL 0045 840 006
DO Electrolyte, Mettler Toledo®
pack of 3 x 25 mL 78108053
Membrane Kit, for 12 mm DO sensors, Hamilton®, including spare o-rings, 3 membrane bodies 78108042
Membrane Kit, for 12 mm DO sensors, Mettler Toledo®, including spare o-rings, 4 membrane bodies 78108003
Membrane Kit, for 12 mm DO sensors, including spare o-rings, Mettler Toledo®
4 membrane bodies P0720-6268
1 membrane body P0720-6339
Membrane Kit, for 12 mm DO sensors, including spare o-rings, Hamilton®, 3 membrane bodies P0720-6570
Sensor Cap, Optocap BT O2T, for Mettler Toledo® InPro® 6860i P0720-6621
Dust Cap, for DO sensor, with 4-pin connector (T82) P0720-5567

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
158

Accessories

pH Sensors
pH Sensors
>> Electrodes for accurate monitoring of pH
>> Various sensor lengths available

Model
Vessel Size Sensor DASbox® Spinner Spinner 1 L/ Spinner Bioreactor Bioreactor Photo­
Group 0.25 L 0.7 L/ Stirrer 1.5 L 1.5 L/ 2.5 L 3.5 L Bioreactor
Stirrer 1 L Stirrer 1.8 L 1.0 L /2.5 L
DASbox® A, C 120 mm - - - - - -
MiniBioreactor
DASGIP® Bioblock A, C - 225 mm 225 mm 325 mm - - -
DASGIP® Benchtop A, C - - - - 225 mm 325 mm -
DASGIP® A, C - - - - - - 225 mm
PhotoBioreactors
SciVario® twin A, B, C, D - 225 mm 225 mm 325 mm 225 mm 325 mm -

Model
Vessel Size Sensor Group BioBLU® BioBLU® 1c/f BioBLU® BioBLU® 5p BioBLU® BioBLU® 50c
0.3c/f/sc 3c/5c/3f 10c/14c
DASbox® A, C 120 mm - - - - -
MiniBioreactor
DASGIP® Bioblock A, C - 225 mm - - - -
DASGIP® Benchtop A, C - 225 mm 225 mm - - -
SciVario® twin A, B, C, D - 225 mm 225 mm - 425 mm 625 mm

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 159

Accessories

Ordering information
Sensor
Description Group Order no.
pH Sensor, Mettler Toledo® 405-DPAS-SC-K8S, autoclavable, O.D. 12 mm A
L 120 mm 78103207
L 225 mm 78103220
L 325 mm 78103209
L 425 mm 78103227
pH Sensor, Mettler Toledo® InPro 3253i, ISM®, autoclavable, O.D. 12 mm B
L 225 mm 78103236
L 325 mm 78103239
pH Sensor, Hamilton® EasyFerm Plus, autoclavable, O.D. 12 mm C
L 120 mm 78103205
L 225 mm 78103230
L 325 mm 78103231
L 425 mm 78103232
L 625 mm 78108075
pH Sensor, Hamilton® EasyFerm Plus ARC, autoclavable, O.D. 12 mm D
L 225 mm 78103237
L 325 mm 78103242

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
160

Accessories

pH Sensors
Model
Vessel Size Sensor Group BioBLU® BioBLU® 1c/f BioBLU® BioBLU® 5p BioBLU® BioBLU® 50c
0.3c/f/sc 3c/5c/3f 10c/14c
BioFlo®/CelliGen 115 E - - 225 mm 120 mm 425 mm 625 mm
BioFlo® 120 E, F - 225 mm 225 mm 120 mm 425 mm 625 mm
BioFlo®/CelliGen 310 E - - 225 mm 120 mm 425 mm 625 mm
BioFlo® 320 E, F - 225 mm 225 mm 120 mm 425 mm 625 mm
CelliGen BLU - E - - 225 mm 120 mm 425 mm 625 mm
1st generation
CelliGen BLU - E - - 225 mm 120 mm 425 mm 625 mm
2nd generation

Model
Vessel Size Sensor 1L 2L 3L 5L 10 L 15 L All Vessels
Group
BioFlo®/CelliGen 115 E 200 mm 225 mm - 325 mm 425 mm - -
BioFlo® 120 E, F 200 mm 225 mm - 325 mm 425 mm - -
BioFlo®/CelliGen 310 E 200 mm - 225 mm 325 mm 425 mm - -
BioFlo® 320 E, F 200-225 mm - 225 mm 325 mm 425 mm - -
CelliGen 310 E 200 mm - 200-225 mm 200 mm 225 mm - -
(packed-bed)
BioFlo® 320 E, F 200-225 mm - 200-225 mm 200-225 mm 225 mm - -
(packed-bed)
BioFlo® 415 E - - - 325 mm 425 mm 625 mm -
BioFlo®/CelliGen 510 E, F * - - - - - - 120 mm
BioFlo® 610 E, F * - - - - - - 120 mm
BioFlo® 720 E, F - - - - - - 225 mm
BioFlo®/CelliGen Pro E, F * - - - - - - Standard
Housing -
125 mm
Retractable
Housing -
320 mm
* Depending on option installedw

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 161

Accessories

Ordering information
Sensor
Description Group Order no.
pH Sensor, Mettler Toledo® 405-DPAS-SC-K8S, gel-filled E
L 120 mm P0720-5581
L 200 mm P0720-5582
L 225 mm P0720-5584
L 325 mm P0720-5580
L 425 mm P0720-5583
pH Sensor, Hamilton® EasyFerm Plus, gel-filled
L 625 mm P0720-6540
pH/Redox Sensor, Mettler Toledo® InPro 3253i, ISM® F
L 120 mm P0720-6656
L 225 mm P0720-6657
L 325 mm P0720-6658
L 425 mm P0720-6659

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
162

Accessories

pH Sensors
Adaptor/
Sensor
Model Cable Housing
Analog Digital Digital
Headplate Side-Wall Sensor
AK9 AK9 (ISM) VP8 (ARC) Adaptor Adaptor Housing
DASbox® MiniBioreactor 78522020 – – – – -
DASGIP® Parallel Bioreactor 78522020 – – 771020161) – -
System
SciVario® twin 7600221501 760020001 760020002 771020161) – -
BioFlo®/CelliGen 115 P0720-2276 – – M1273-5040 – -
BioFlo® 120 1390810400 M1379-8108 – M1273-5040 – -
BioFlo®/CelliGen 310 P0720-2273 – – M1287-5030 – -
BioFlo® 320 M1379-8104 M1379-8108 – M1287-5030 – -
BioFlo® 415 P0720-2273 – – – – -
CelliGen BLU - 1st Generation N/A – – – – -
CelliGen BLU - 2nd Generation P0720-2276 – – – – -
BioFlo®/CelliGen 510 P0720-2276 – – – M1361-9208 P0720-
6240C3
BioFlo® 610 P0720-2276 – – – M1361-9208 P0720-
6240C3
BioFlo® 720 1385810400 1385810800 – – – 0045890038
BioFlo®/CelliGen Pro - Standard M1290-0610 – – – – P0720-
Housing 6450C1
BioFlo®/CelliGen Pro - M1290-0610 – – – – P0720-5570C
Retractable Housing and M1290-
8012
1)
For DASGIP / SciVario twin Benchtop Bioreactors

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 163

Accessories

Ordering information
Description Order no.
Sensor Cable, for connecting pH/Redox sensors to DASGIP® modules, grey, with plug type AK9, L 3 m (9.8 ft) 78522020
Sensor Cable, for analog pH sensors, for SciVario® twin, with plug type AK9 7600 221 501
Sensor Cable, for ISM® sensors, for SciVario® twin, with plug type AK9 7600 220 001
Sensor Cable, for ARC® sensors, for SciVario® twin, with plug type VP8 7600 220 002
Sensor Cable, for analog pH sensors, for BioFlo®/CelliGen 115, BioFlo®/CelliGen 510 and BioFlo® 610, with plug type P0720-2276
AK9, L 1.4 m (4.6 ft)
Sensor Cable, for analog pH sensors, controller with Lumberg® connector, with plug type AK9, L 3 m (9.8 ft) 1385 810 400
Sensor Cable, for ISM® sensors, controller with Lumberg® connector, with plug type AK9, L 3 m (9.8 ft) 1385 810 800
Sensor Cable, for analog pH sensors, for BioFlo®/CelliGen 310, BioFlo® 410, BioFlo® 415, with plug type AK9, L 1 m (3.3 ft) P0720-2273
Sensor Cable, for analog pH/Redox sensors, for BioFlo® 120, with plug type AK9, L 1 m (3.3 ft) 1390 810 400
Sensor Cable, for analog pH sensors, for BioFlo® 320, with plug type AK9, L 1 m (3.3 ft) M1379-8104
Sensor Cable, for ISM® sensors, with plug type AK9, L 1 m (3.3 ft) M1379-8108
Sensor Cable, for analog pH/Redox sensors, for BioFlo®/CelliGen Pro, with plug type AK9, L 1.8 m (5.9 ft) M1290-0610
Cable, pH/redox ground wire extension, for retractable sensor housings, 0.6 m M1290-8012
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for BioBLU® 1/3, 216 mm 78703017
shaft, L 2.0 m
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for BioBLU® 1c/3c/5c P0300-2371
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for BioBLU® 10c/14c P0300-2370
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for BioBLU® 50c P0300-2374
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for BioBLU® 5p P0300-2372
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for BioBLU® 0.3, 115.5 mm 78703030
shaft, L 1.5 m
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for SciVario® twin, for 7600 229 001
BioBLU® 1/3, 216 mm shaft
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for SciVario® twin, for 7600 229 002
BioBLU® 10, 346 mm shaft
Polymer Optical Fiber, for optical pH measurement in BioBLU® Single-Use Bioreactors, for SciVario® twin, for 7600 229 003
BioBLU® 50, 504 mm shaft
Sensor Housing, stainless steel
for 25 mm Ingold® port, for 120 mm sensor length, with material certificate P0720-6450C1
InFit® 761, for 25 mm Ingold® port, for 120 mm sensor length, with material certificate P0720-6240C3
retractable, InTrac® 797, for 25 mm Ingold® port, 325 mm sensor length, with material certificate P0720-5570C
Ingold® Port Weldment, converts 1 1/2 in sanitary to 25 mm Ingold® port M1361-9208
Sensor Sleeve Bellow, for attaching sensor to SUB, with AseptiQuik® connector 0045 890 038
Adaptor
Pg 13.5 female thread to M18x1.5 male thread 77102016
compression fitting, 12 mm port to 12 mm sensor M1273-5040
compression fitting, Pg 13.5 port to 12 mm sensor M1287-5030

Accessories
Description Order no.
Dust Cap, for sensor, K9 connector P0720-5317

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
164

Accessories

Redox Sensors
Redox Sensors
>> Accurate monitoring of ORP (redox potential)
>> Used with DASGIP/DASbox PHPO monitoring module series and
Eppendorf control units, respectively
>> Various sensor lengths available

Model
Vessel Size Sensor DASbox® Spinner Spinner 1 L/ Spinner Bioreactor Bioreactor Photo­
Group 0.25 L 0.7 L/ Stirrer 1.5 L 1.5 L/ 2.5 L 3.5 L Bioreactor
Stirrer 1 L Stirrer 1.8 L 1.0 L /2.5 L
DASbox® A 120 mm - - - - - -
MiniBioreactor
DASGIP® Bioblock A - 225 mm 225 mm 325 mm - - -
DASGIP® Benchtop A - - - - 225 mm 325 mm -
DASGIP® A - - - - - - 225 mm
PhotoBioreactors

Model
Vessel Size Sensor Group BioBLU® BioBLU® 1c/f BioBLU® BioBLU® 5p BioBLU® BioBLU® 50c
0.3c/f/sc 3c/5c/3f 10c/14c
DASbox® A 120 mm - - - - -
MiniBioreactor
DASGIP® Bioblock A - 225 mm - - - -
DASGIP® Benchtop A - 225 mm 225 mm - - -

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Accessories

Ordering information
Sensor
Description Group Order no.
Redox Sensor, Mettler Toledo® Pt4805-DPAS-SC-K8S, autoclavable, O.D. 12 mm A
L 120 mm 78103224
L 225 mm 78103225
L 325 mm 78103226

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
166

Accessories

Redox Sensors
Model
Vessel Size Sensor Group BioBLU® BioBLU® 1c/f BioBLU® BioBLU® 5p BioBLU® BioBLU® 50c
0.3c/f/sc 3c/5c/3f 10c/14c
BioFlo®/CelliGen 115 B - - 225 mm 225 mm 325 mm 625 mm
BioFlo® 120 B, C - 225 mm 225 mm 225 mm 325 mm 625 mm
BioFlo®/CelliGen 310 B - - 225 mm 225 mm 325 mm 625 mm
BioFlo® 320 B, C - 225 mm 225 mm 225 mm 325 mm 625 mm

Model
Vessel Size Sensor 1L 2L 3L 5L 10 L 15 L All Vessels
Group
BioFlo®/CelliGen 115 B 225 mm 225 mm - 325 mm 425 mm - -
BioFlo® 120 B, C 225 mm 225 mm - 325 mm 425 mm - -
BioFlo®/CelliGen 310 B 225 mm - 225 mm 325 mm 425 mm - -
BioFlo® 320 B, C 225 mm - 225 mm 325 mm 425 mm - -
CelliGen 310 B 225 mm - 225 mm 225 mm 225 mm - -
(packed-bed)
BioFlo® 320 B, C 225 mm - 225 mm 225 mm 225 mm - -
(packed-bed)
BioFlo® 415 B - - - 325 mm 425 mm 625 mm -
BioFlo®/CelliGen 510 B, C * - - - - - - 120 mm
BioFlo® 610 B, C * - - - - - - 120 mm
BioFlo 720
®
B, C - - - - - - 225 mm
BioFlo®/CelliGen Pro B, C * - - - - - - Standard
Housing -
120 mm
Retractable
Housing -
325 mm
* Depending on option installedw

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Accessories

Ordering information
Sensor
Description Group Order no.
Redox Sensor, Mettler Toledo® Pt4805-DPAS-SC-K8S, gel-filled B
L 120 mm P0720-5780
L 225 mm P0720-5783
L 325 mm P0720-5782
L 425 mm P0720-5784
Redox Sensor, Hamilton® EasyFerm RX, gel-filled
L 325 mm P0720-6532
L 425 mm P0720-6531
L 625 mm P0720-6530
pH/Redox Sensor, Mettler Toledo® InPro 3253i, ISM® C
L 120 mm P0720-6656
L 220 mm P0720-6657
L 320 mm P0720-6658
L 420 mm P0720-6659

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
168

Accessories

Redox Sensors
Model Cable Adaptor/Sensor
Analog Digital
AK9 ISM Headplate Side-Wall Sensor Housing
Adaptor Adaptor
DASbox® MiniBioreactor 78522020 – – – –
DASGIP® Parallel Bioreactor 78522020 – 77102016 1) – –
System
BioFlo®/CelliGen 115 P0720-2763 – M1273-5040 – –
BioFlo® 120 1390810400 M1379-8108 M1273-5040 – –
BioFlo®/CelliGen 310 P0720-2275 – M1287-5030 – –
BioFlo® 320 M1379-8105 M1379-8108 M1287-5030 – –
BioFlo® 415 P0720-2275 – – – –
BioFlo®/CelliGen 510 P0720-2277 – – M1361-9208 P0720-6240C3
BioFlo® 610 P0720-2277 – – M1361-9208 P0720-6240C3
BioFlo® 720 1385810500 1385810800 – – 0045890038
BioFlo®/CelliGen Pro - Standard M1290-0610 – – – P0720-6450C1
housing
BioFlo®/CelliGen Pro - M1290-0610 & – – – P0720-5570C
Retractable housing M1290-8012
1)
For DASGIP Benchtop Bioreactors

Ordering information
Description Order no.
Sensor Cable, for connecting pH/Redox sensors to DASGIP® modules, grey, with plug type AK9, L 3 m (9.8 ft) 78522020
Sensor Cable, for analog pH/Redox sensors, for BioFlo® 120, with plug type AK9, L 1 m (3.3 ft) 1390 810 400
Sensor Cable, for analog Redox sensors, for BioFlo® 320, with plug type AK9, L 1 m (3.3 ft) M1379-8105
Sensor Cable, for ISM® sensors, with plug type AK9, L 1 m (3.3 ft) M1379-8108
Sensor Cable, for analog Redox sensors, controller with Lumberg® connector, with plug type AK9, L 3 m (9.8 ft) 1385 810 500
Sensor Cable, for ISM® sensors, controller with Lumberg® connector, with plug type AK9, L 3 m (9.8 ft) 1385 810 800
Sensor Cable, for analog Redox sensors, for BioFlo® 110 and BioFlo®/CelliGen 115, with plug type AK9, L 1.8 m (5.9 ft) P0720-2763
Sensor Cable, for analog Redox sensors, for BioFlo®/CelliGen 310 and BioFlo® 415, with plug type AK9, L 1 m (3.3 ft) P0720-2275
Sensor Cable, for analog Redox sensors, for BioFlo®/CelliGen 510 and BioFlo® 610, with plug type AK9, L 1.4 m (4.6 ft) P0720-2277
Sensor Cable, for analog pH/Redox sensors, for BioFlo®/CelliGen Pro, with plug type AK9, L 1.8 m (5.9 ft) M1290-0610
Cable, pH/redox ground wire extension, for retractable sensor housings, 0.6 m M1290-8012
Transmitter, Mettler Toledo® M300
redox, panel mounted P0620-5974
redox, wall mounted P0620-5975
Secondary DO-pH/Redox Option, for BioFlo®/CelliGen 310 and BioFlo® 415, field-installed M1287-3530
Sensor Housing, stainless steel
for 25 mm Ingold® port, for 120 mm sensor length, with material certificate P0720-6450C1
InFit® 761, for 25 mm Ingold® port, for 120 mm sensor length, with material certificate P0720-6240C3
retractable, InTrac® 797, for 25 mm Ingold® port, 325 mm sensor length, with material certificate P0720-5570C
Ingold® Port Weldment, converts 1 1/2 in sanitary to 25 mm Ingold® port M1361-9208
Sensor Sleeve Bellow, for attaching sensor to SUB, with AseptiQuik® connector 0045 890 038
Adaptor
Pg 13.5 female thread to M18x1.5 male thread 77102016
compression fitting, 12 mm port to 12 mm sensor M1273-5040
compression fitting, Pg 13.5 port to 12 mm sensor M1287-5030

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Accessories

Turbidity Sensors
Ordering information
Description Order no.
Turbidity Sensor, Mettler Toledo® InPro® 8100, autoclavable, 297 mm (cable not included) P0720-5950
Turbidity Sensor, Mettler Toledo® InPro® 8200
SIP, 407 mm (with cable) P0720-5960
SIP, 120 mm (with cable) P0720-5961
SIP, 205 mm (with cable) P0720-5962
SIP, 297 mm (with cable) P0720-5963
Turbidity Cable, 5 m P0720-2430
Transmitter, Mettler Toledo® M800, one-channel P0620-6571

CO2 Sensors
Ordering information
Description Order no.
CO2 Sensor, Mettler Toledo® InPro® 5000i, ISM®
120 mm P0720-6663
220 mm P0720-6664
320 mm P0720-6665
Transmitter, Mettler Toledo® M800, one-channel P0620-6571
Sensor Cable, for CO2 sensors, for Mettler Toledo® transmitter, with plug type VP, L 3 m (9.8 ft) P0720-9660
Sensor Cable, for ISM® sensors, with plug type AK9, L 1 m (3.3 ft) M1379-8108
CO2 Membrane Kit, including spare o-rings and electrolyte, Mettler Toledo® InPro® 5000/InPro® 5000i, 4 membrane P0720-6641
bodies

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
170

Accessories

Optical Density Sensors

Optical Density Sensors
>> Accurate measurement of optical absorbance
>> Used with the DASGIP OD4 monitoring module
>> Different optical path lengths for various applications
>> Various sensor lengths available

Model DASGIP® OD Sensor

Optical path length 5 mm 5 mm 5 mm 10 mm 10 mm 10 mm 20 mm 20 mm 20 mm
781 781 781 781 781 781 781 781 781
Order no. 03411 03408 03414 03412 03409 03415 03413 03410 03416
120 225 335 120 335 335
Sensor length mm mm mm mm 225 mm mm 120 mm 225 mm mm
DASbox® MiniBioreactor   
DASGIP® Benchtop Spinner 0.5 L   
DASGIP® Benchtop Spinner 1.5 L   
DASGIP® Bioblock Spinner 0.7 L/Stirrer 1 L   
DASGIP® Bioblock Spinner 1 L/Stirrer 1.5 L   
DASGIP® Bioblock Spinner 1.5 L/Stirrer 1.8 L   
DASGIP® Benchtop Bioreactors 2.5 L   
DASGIP® Benchtop Bioreactors 3.5 L   
DASGIP® PhotoBioreactors 1.0 L   
DASGIP® PhotoBioreactors 2.5 L   

Accessories
Description Order no.
Compression Fitting, complete, with M18x1.5 male thread, I.D. 12 mm 78532281
Compression Fitting, complete, with Pg 13.5 male thread, I.D. 12 mm 78532284
OD Cable, for connecting OD sensors to DASGIP® modules, L 3 m 78522037

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

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Accessories

Level Sensors
Level Sensors
>> Activation of pumps for level control due to level changes
>> Anti foam addition due to foam build-up
>> Used with the DASGIP PHPO monitoring modules with level option

Ordering information
Description Order no.
Level Sensor, stainless steel with PFA coating
L 130 mm, Li 20 – 90 mm 78103145
L 200 mm, Li 20 – 160 mm 78103146
L 230 mm, Li 20 – 190 mm 78103147
Li = immersion depth

Accessories
Description Order no.
Compression Fitting, complete, with M18x1.5 male thread, I.D. 4 mm 78532279
Compression Fitting, complete, with Pg 13.5 male thread, I.D. 4 mm 78532282
Level Sensor Cable, L 3 m 78522031

Temperature Sensors
Temperature Sensors
>> Platinum RTD temperature sensors (Pt100)
>> Designed for use with DASGIP bioreactors

Ordering information
Description Order no.
Platinum RTD Temperature Sensor, 100 Ohm class A
O.D. 1.6 mm, L 150 mm, cable L 1.8 m 78103314
O.D. 1.6 mm, L 300 mm, cable L 1.3 m 78103308
Platinum RTD Temperature Sensor, 100 Ohm class A, O.D. 1.6 mm, L 300 mm, cable L 3 m 78103304
O.D. 1.6 mm, L 400 mm, cable L 3 m 78103307
Platinum RTD Temperature Sensor, 100 Ohm class 1/10b
O.D. 4.78 mm, L 111 mm, cable L 1.35 m (for BioBLU® 3c/5c/5p) 78103319
Temperature Sensor (RTD), for SciVario® twin
for BioBLU® 1 Single-Use Vessels, L 215 mm, O.D. 2.5 mm 7600 223 011
for BioBLU® 3 Single-Use Vessels, L 250 mm, O.D. 4.5 mm 7600 223 012
for BioBLU® 10 Single-Use Bioreactors, L 340 mm, O.D. 4.5 mm 7600 223 015
for BioBLU® 50 Single-Use Bioreactors, L 495 mm, O.D. 4.5 mm 7600 223 016
for glass vessels, L 300 mm, O.D. 1.6 mm 7600 223 002
for glass vessels, L 400 mm, O.D. 1.6 mm 7600 223 003

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
172

Service

As with all complex technical systems, Eppendorf bioprocess equipment should be maintained regularly to

keep all parts in good working order. This maintenance avoids cost-intensive down times

and contributes to preservation of value.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 173

>> DASbox® Mini Bioreactor System Performance Plans 174 - 175

>> DASGIP® PHPO and OD4 Performance Plans 176 - 177
>> DASGIP® GA Performance Plans 178 - 179
>> DASGIP® TC4SC4 Performance Plans 180 - 181
>> DASGIP® MP8 and MP4 Performance Plans 182 - 183
>> DASGIP® MX4/4 Performance Plans 184 - 185
>> DASGIP® MX4/1, MF4 and Rotameters Performance Plans 186 - 187
>> DASGIP® PBR4 Performance Plans 187 - 189
>> DASGIP® Control System Performance Plans 190 - 191
>> SciVario® twin Performance Plans 192 - 193
>> DASGIP®, DASbox®, and SciVario® twin Vessels Performance Plans 194 - 195
>> BioFlo® 120 Performance Plans 196 - 197
>> BioFlo®/CelliGen 115 Performance Plans 198 - 199
>> BioFlo® 320 Performance Plans 200 - 201
>> BioFlo®/CelliGen 310 Performance Plans 202 - 203
>> CelliGen BLU Performance Plans 204 - 205
>> BioFlo® 415 Performance Plans 206 - 207
>> BioFlo®/CelliGen 510 (RPC/AB) Performance Plans 208 - 209
>> BioFlo® 610 Performance Plans 210 - 211
>> BioFlo® 720 Performance Plans 212 - 213
>> BioFlo® Pro Performance Plans 214 - 215
>> CelliGen Pro Performance Plans 216 - 217

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
174

Service

DASbox® Mini Bioreactor System Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The Eppendorf DASbox is the solution for bioprocessing in small working volumes. In small volumes
precision and process reliability are essential. Slight deviations or irregularities influence the process and
the cultivated cells and organisms much more than at larger scales. Be on the safe side with calibrated and
adjusted mass flow sensors, sensor electronics and actuators.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 175

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)*
Order Number 0082 150 373 0082 150 374 0082 150 375 0082 150 379
External Inspection
General condition of DASbox® and all attached ■ ■ ■ ■
cables
General condition of tubing ■ ■ ■
Internal Inspection  
Check/update of firmware and internal battery ■ ■ ■
Check of internal settings ■ ■ ■ ■
Functional Check  
Operational check of all actuators ■ ■ ■
(heating, cooling, gassing, pumps)
Operational check of exhaust condenser ■ ■ ■
Operational check of sensor cables ■ ■
Operational check of all attached sensors ■
Verification
Verification of sensor performance ■ ■
Verification of agitation speed ■ ■ ■
Verification of gas flow ■
Verification of temperature measurement ■ ■ ■
Verification of exhaust performance ■ ■
Calibration and Adjustment
Calibration and adjustment of mass flow sensors ■ ■
Calibration of electronics of sensors for pH, DO, ■ ■ ■ ■
level, redox, temperature**
Documentation    
Check list provided ■ ■ ■ 
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
* Separate purchase of IQ/OQ documents will be necessary
**Where feature is present

For a complete mainenance of your DASbox® additional services are recommended:

Control system: Small scale vessel:
> Essential Check: 0082 150 003 > Essential Check: 0082 150 333
> Advanced Maintenance: 0082 150 004 > Advanced Maintenance: 0082 150 334
> Premium Service: 0082 150 005 > Premium Service: 0082 150 335

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
176

Service

DASGIP® PHPO and OD4 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The most common reasons for failed experiments are incorrect sensor readings or defective sensors.
Therefore, it is essential to identify and eliminate these risks before they arise. Preventive maintenance of
electronics, cables, and sensors notably diminishes in-process failure.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 177

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)*
Order Number (for DASGIP® PHPO) 0082 150 453 0082 150 454 0082 150 455 0082 150 459
Order Number (for DASGIP® OD4) 0082 150 043 0082 150 044 0082 150 045 0082 150 049
External Inspection
General condition of DASGIP® module and attached ■ ■ ■ ■
cables
Internal Inspection   
Check/update of firmware ■ ■ ■
Check/update of internal battery ■ ■ ■
Check of internal settings ■ ■ ■ ■
Functional Check  
Operational check of sensor cables ■ ■
Operational check of all attached sensors ■  
Verification
Verification of sensor performance  ■ ■
Calibration and Adjustment
Calibration and adjustment of electronics of sensors ■ ■ ■ ■
for pH, DO, level, redox, temperature**
1-point calibration of all attached sensors ■   
2-point calibration of all attached sensors ■ ■ 
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Cost of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
*Separate purchase of IQ/OQ documents will be necessary
**Where feature is present

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
178

Service

DASGIP® GA Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The DASGIP GA4 exhaust analyzer supports precise online measurement of exhaust oxygen and carbon
dioxide in four discrete analyzer channels. The online monitoring of oxygen transfer rate (OTR), carbon
dioxide transfer rate (CTR), and respiratory quotient (RQ) permits direct conclusions on the metabolic state
of the culture and allows implementation of online feedback loops. As the GA4 gas sensors have a limited
working lifetime, regular maintenance is essential to obtaining reliable experimental data.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 179

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 203 0082 150 204 0082 150 205 0082 150 209
External Inspection
General condition of DASGIP® GA4 module and ■  ■ ■ ■
attached cables
Internal Inspection   
Check/update of firmware ■ ■ ■
Check/exchange of internal battery  ■ ■  ■
Check of internal settings  ■ ■ ■ ■
Check lifetime of gas sensors  ■ ■ ■
Verification  
Characterization of gas sensor performance with air ■ ■  ■ ■
Characterization of gas sensor performance with test  ■ ■  ■  ■
gas2)
Characterization of mass flow sensor performance ■ ■ ■ ■
Calibration and Adjustment   
Calibration and adjustment of mass flow sensors  ■  ■
Calibration and adjustment of ambient pressure  ■  
■
sensor
1-point calibration of gas sensors with air ■   
2-point calibration of gas sensors with air and test ■ ■ ■
gas2)
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service  ■  ■  ■ ■
IQ/OQ report and certificate  ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repair/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
1)
Seprarate purchase of IQ/OQ documents will be necessary
2)
Test gas has to be provided by customer

Order numbers for additional, inhouse services:

GA4: GA4E:
> GA4 sensor train inspection (O2/CO2): 77 105 000 > GA4E sensor train inspection (O2/CO2): 77 105 039
> GA4 sensor train maintenance (O2/CO2/flow): 77 105 001 > GA4E sensor train maintenance (O2/CO2/flow): 77 105 040
> GA4 maintenance (4x O2/CO2): 77 105 002 > GA4E maintenance (4x O2/CO2): 77 105 041

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
180

Service

DASGIP® TC4SC4 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
Efficient mixing and accurate temperature control are indispensable to achieve optimum growth
conditions, especially for mass and heat transfer in microbial cultivation. Mammalian cells require smooth
mixing with low shear stress. The DASGIP TC4SC4 module provides individual temperature and agitation
controls for four bioreactors.
Their correct functioning is essential for every experiment, because even the smallest deviations can
disrupt the experiment, as well as further process development and upscaling. Therefore, regular
verification and calibration of motors, temperature sensors, and heating and cooling devices is
indispensable for standardized process conditions.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 181

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 413 0082 150 414 0082 150 415 0082 150 419
External Inspection
General condition of DASGIP® TC4SC4 module and ■ ■ ■ ■
attached cables
Internal Inspection   
Check/update of firmware ■ ■ ■
Check/exchange of internal battery ■ ■ ■
Check of internal settings ■ ■ ■ ■
Functional Check  
Operational checks of heating ■ ■ ■ ■
Operational check of cooling ■ ■ ■ ■
Operational check of agitation ■ ■ ■ ■
Verification
Verification of temperature sensor performance ■ ■ ■ ■
Verification of heating performance ■ ■ ■
Verification of cooling performance ■ ■ ■
Verification of agitation performance ■ ■ ■
Calibration
Calibration and adjustment of temperature ■ ■ ■
electronics
Calibration and adjustment of temperature sensors ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Cost of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
1)
Separate purchase of IQ/OQ documents will be necessary

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
182

Service

DASGIP® MP8 and MP4 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
Microbial and cell culture applications require precise flows of feed and buffer. The DASGIP variable-speed
pump modules MP4 and MP8 provide four or eight high-precision, speed-controlled miniature peristaltic
pumps, respectively.
Our field service engineers ensure accurate functioning of the pumps and accessories with scheduled
preventive maintenance. They take equipment usage and condition into account with predictive
maintenance techniques to determine when service will be required, thus saving money and downtime.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 183

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 083 0082 150 084 0082 150 085 0082 150 089
External Inspection
General condition of DASGIP® MP module, attached ■ ■ ■ ■
cables, and feed lines2)
Internal Inspection   
Check/update of firmware ■ ■ ■
Check/exchange of internal battery ■ ■ ■
Check for correct calibration parameters ■ ■
Functional Check  
Operational check of all pumps (manual mode) ■ ■ ■ ■
Operational check of all pumps (automatic mode) ■ ■ ■ ■
Verification
Verification of pump and pump head condition ■ ■ ■
Verification of tightness of the feed line manifold2) ■ ■
Verification of tightness of the feed lines2) ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Cost of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
Separate purchase of IQ/OQ documents will be necessary
1)

Where feed lines are available

2)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
184

Service

DASGIP® MX4/4 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The DASGIP MX4/4 gas mixing system supplies four separate culture vessels with independent mixtures
of air, N2, O2, and CO2. Each gas outlet has separate setpoints for flow rate, and concentrations of O2 and
CO2. Regular maintenance is crucial to ensuring reproducible and reliable gas mixtures for your cell
culture applications and microbial fermentations. Fast and precise calibration and adjustment of your gas
mixing system can be performed on-site, which minimizes down time.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 185

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 123 0082 150 124 0082 150 125 0082 150 129
External Inspection
General condition of DASGIP® MX module and ■ ■ ■ ■
attached cables
Internal Inspection   
Check/update of firmware ■ ■ ■
Check/exchange of internal battery ■ ■ ■
Check of internal settings ■ ■ ■ ■
Functional Check  
Operational check of inline gas filter ■ ■ ■
Operational check of alarm functions on all ■ ■ ■ ■
channels/gasses
Operational check of gas inlets/outlets ■ ■ ■ ■
Verification
Verification of valve resistance  ■ ■
Characterization of mass flow sensor performance ■ ■ ■
Verification of gassing system tightness  ■ ■
Calibration and Adjustment    
Calibration and adjustment of inline pressure ■ ■ 
sensors
Calibration and adjustment of valve current of flow ■ ■
valves
Calibration and adjustment of mass flow sensors ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repair/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
Separate purchase of IQ/OQ documents will be necessary
1)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
186

Service

DASGIP® MX4/1, MF4 and Rotameters

Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The DASGIP MX4/1 gas mixing system supplies one culture vessel with an individual mixture of air, N2, O2,
and CO2. The DASGIP MF4 gassing module allows mass flow-controlled gassing of up to four vessels with
individual flow rates. DASGIP Rotameters WRM and RX2/4 provide four-channel rotameter gassing at an
adjustable flow rate from one and two gas supplies, respectively. Regular maintenance is crucial to ensure
a reproducible and reliable gas supply. Fast calibration of your gassing system can be performed on-site,
which minimizes down time.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 187

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 163 0082 150 164 0082 150 165 0082 150 169
External Inspection
General condition of DASGIP® module ■ ■ ■ ■
General condition of all attached cables, tubes, and ■ ■ ■ ■
labels
Internal Inspection  
Check/update of firmware ■ ■ ■
Check/exchange of internal battery ■ ■ ■
Check of internal settings and parameters ■ ■ ■ ■
Inspection of flow controller2) ■ ■ ■ ■
Check of alarm settings ■ ■ ■ ■
Check system tightness ■ ■ ■ ■
Functional Check  
Operational check of pressure sensor and pressure ■ ■ ■ ■
control2)
Calibration
2-point calibration of WRM or RX2/42) ■
4-point calibration of WRM or RX2/42) ■ ■
4-point calibration of MX4/1 or MF42) ■
8-point calibration of MX4/1 or MF42) ■ ■
Documentation    
Check list provided ■ ■ ■ 
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
1)
Separate purchase of IQ/OQ documents will be necessary
2)
Where feature is present

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
188

Service

DASGIP® PBR4 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
Phototrophic organisms use different types of chlorophyll. To address their individual requirements, the
DASGIP PBR4 enables illumination of photobioreactors with selected wavelengths, ideally matched to
the absorption maxima of relevant chlorophyll molecules. The DASGIP PBR4 module provides parallel
illumination of up to 4 bioreactors under individual conditions. By selectively varying the light intensities
of different wavelength channels, both the spectral composition and the overall intensity of the resulting
light can be adjusted according to individual requirements. In addition to a continuous illumination mode
the DASGIP PBR4 module supports the configuration of variable day and night cycles as well as the
programming of different flash modes.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 189

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 293 0082 150 294 0082 150 295 0082 150 299
External Inspection
General condition of DASGIP® PBR4 module and all ■ ■ ■ ■
attached cables
Internal Inspection  
Check/update of firmware ■ ■ ■
Check/exchange of internal battery ■ ■ ■
Check of internal settings ■ ■ ■ ■
Functional Check  
Operational check of LED cables ■ ■ ■ ■
Operational check of all attached illuminators ■ ■ ■ ■
Operational check of all LED types ■ ■ ■ ■
Operational check of illumination modes ■ ■ ■
Verification
Verification of photon intensity values ■ ■
Documentation    
Check list provided ■ ■ ■ 
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
1)
Separate purchase of IQ/OQ documents will be necessary

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
190

Service

DASGIP® Control System Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The DASGIP control system comprises a process computer and the bioprocess control software DASware®
control. It is at the core of all DASGIP Parallel Bioreactor Systems and the DASbox® Mini Bioreactor
System. Operating multiple bioreactors in parallel reduces the number of runs needed and therefore
accelerates process development. DASware control facilitates the control of up to 24 bioreactors in
parallel. Combined with extensive embedded process automation features, intelligent recipe management,
and integrated report generating capabilities it offers an unprecedented level of integral process
documentation.
Our field service engineers ensure accurate functioning of your process computer, implement updates of
DASware control, and maintain your database, to ensure the best performance of your control system.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 191

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 003 0082 150 004 0082 150 005 0082 150 009
External Inspection
General condition of DASGIP® process computer ■ ■ ■ ■
General condition of all attached cables ■ ■ ■ ■
Internal Inspection  
Check database size ■ ■ ■
Check DTP-Insight firmware version ■ ■ ■ ■
Check process computer settings ■ ■ ■ ■
Check/update of firmware ■ ■ ■ ■
Update of minor release ■ ■
Functional Check  
Check automatic export function ■ ■ ■ ■
Perform database export ■ ■ ■
UPS capacity check2) ■ ■
Assistance
Scripting support ■
Documentation    
Check list provided ■ ■ ■ 
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
1)
Separate purchase of IQ/OQ documents will be necessary
2)
Where feature is present

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
192

Service

SciVario® twin Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires a smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The SciVario twin next-generation bioreactor control station is the first product of our new bioprocess
controller platform SciVario for small- and bench-scale instruments with a new intuitive user-interface
and highly innovative hard- and software solutions. The SciVario twin was developed for the individual
or parallel control of up to two bioreactors (BioBLU® Single-Use Bioreactors and glass vessels). With
the patented baydrawer system, the hardware of the controller can be flexibly adapted to your needs,
without the necessity of purchasing a completely new system.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over the years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 193

Service

Service Operation ESSENTIAL CHECK ADVANCED PREMIUM

MAINTENANCE SERVICE
Order Number 0082 150 770 0082 150 780 0082 150 790
External Inspection and Maintenance
General condition of the controller  
General condition of all attached cables and tubing
General condition of the vessels
General condition of all inserted drawers
General condition of all accessories (temperature
control block, heating blanket, motors)
Verify utility connections
Internal Inspection
Update to latest firmware and software revision
Perform export of experimental data
Functional Check
Operational check of all actuators
(heating, cooling, agitation, gassing, pumps)
Operational check of exhaust condensers
Operational check of sensor cables
Verification
Verification of the sensor performance
Verification of sensor electronics for pH and DO
Verification of the agitation speed
Verification of the temperature measurement
Perform software test run
Documentation
Check list provided
Dated service sticker to confirm Eppendorf service
Supporting Information
Contract period one year one year one year
Number of preventive services included one one one
Cost of repairs/parts replaced outside scope of not included not included discount on parts, labor,
preventive maintenance visit (where Eppendorf travel time
product warranty has expired)

*For a complete maintenance of your SciVario twin Small Scale Vessel DASware control system
system, Small Scale Vessel Maintenance and Essential Check 0082 150 333 0082 150 003
DASware® Control System Maintenance services Advanced Maintenance 0082 150 334 0082 150 004
are recommended.
Premium Service 0082 150 335 0082 150 005

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
194

Service

DASGIP®, DASbox®, and SciVario® twin

Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts.
Regular maintenance by qualified engineers helps to ensure reliable operation, while deferring it can
cause unreliable results, expensive repairs, and prolonged downtime. Eppendorf offers autoclavable,
overhead-driven glass vessels for microbial and cell culture applications. They feature multi-port
stainless steel head plates and Rushton-type or pitched-blade impellers and cover working volumes
of 60 mL – 3.8 L. Stirrer assemblies and head plates are equipped with numerous seals and O-rings to
ensure a safe and sterile connection between the different parts. To ensure culture sterility, it is essential
to exchange them on a regular basis. A preventive maintenance of the vessel and the stirring assembly
notably diminishes contamination and performance problems. Eppendorf Bioprocess Performance Plans
help you to maintain the optimal performance of your equipment over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 195

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation

CHECK MAINTENANCE SERVICE Qualification/
Operational
Qualification
(IQ/OQ)1)
Order Number 0082 150 333 0082 150 334 0082 150 335 0082 150 339
External Inspection
General condition of DASGIP® or DASbox® vessel ■ ■ ■ ■
General condition of all O-rings and seals ■ ■ ■ ■
General conditions of all reusable parts ■ ■ ■ ■
General condition of all vessel filters ■ ■ ■ ■
Maintenance  
Maintenance of stirrer assembly ■ ■ ■
Exchange of all O-rings on vessel ■ ■
Functional Check  
Operational check of vessel ■ ■
Documentation    
Check list provided ■ ■ ■ 
Dated service sticker to confirm Eppendorf service ■ ■ ■ ■
IQ/OQ report and certificate ■
Supporting Information
Contract period one year one year one year n/a
Number of preventive services included one one one n/a
Costs of repairs/parts replacement outside scope not included not included discount on parts, n/a
of preventive maintenance visit (where Eppendorf labor, travel time
product warranty has expired)
1)
Separate purchase of IQ/OQ documents will be necessary

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
196

Service

BioFlo® 120 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The Eppendorf BioFlo 120 offers simplicity and ease of use, without sacrificing capability. No matter if you
are in an academic, governmental or industrial research setting, or working with bacteria, yeast, fungi,
mammalian, insect or plant cells, the BioFlo 120 is an attractive solution to meet your needs. It features
an extensive range of glass and BioBLU® Single-Use Bioreactors options (250 mL – 40 L). Eppendorf
Bioprocess Performance Plans help you to maintain the optimal performance of your equipment over years
of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 197

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation Operational IQ/OQ

CHECK MAINTE- SERVICE Qualification Qualification
NANCE (IQ) (OQ)
Order Number 0082 140 043 0082 140 044 0082 140 045 0082 140 047 0082 140 048 0082 140 049
External Inspection and
Maintenance
Check installation environment ■ ■ ■ ■ ■
Verify utility connections ■ ■ ■ ■ ■
Inspect addition pump heads and ■ ■ ■ ■ ■
motors
Inspect motor cables and sensor ■ ■ ■ ■ ■
cables
Check vessel for damage ■ ■ ■ ■ ■
Internal Equipment
Check motor and bearing housing ■ ■ ■ ■ ■
for speed and operation1)
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, washers, ■ ■
and ferrules
Inspect cooling valve ■ ■ ■
Inspect gas sequencing valves ■ ■ ■
System Checks
Calibrate pH and DO loops with ■ ■ ■ ■ ■ ■
simulator
Check gas flow and mixing ■ ■ ■
Verify motor speed1) ■ ■ ■ ■ ■ ■
Verify temperature ■ ■ ■
Perform test run1) ■ ■ ■ ■ ■ ■
Check for latest firmware revision ■ ■ ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm ■ ■ ■ ■ ■ ■
Eppendorf service
IQ report and signed documentation ■ ■
OQ report and signed ■ ■
documentation
Supporting Information
Contract period one year one year one year n/a n/a n/a
Number of preventive services one one one n/a n/a n/a
included
Cost of repairs/parts replaced not included not included discount on n/a n/a n/a
outside scope of preventive parts, labor,
mainenance visit (where Eppendorf travel time
product warranty has expired)
Note: For customers using BioBLU® Single-Use Bioreactors please select the Essential Check only. Please contact your local Eppendorf representative for other service contract options.
1)
Customers using single-use vessels must provide a vessel for these tests.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
198

Service

BioFlo®/CelliGen 115 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The Eppendorf BioFlo/CelliGen 115 is an easy-to-use benchtop system, with built-in controls for operation
as a microbial fermentor or mammalian cell culture bioreactor.
It features an extensive range of glass and BioBLU Single-Use Bioreactor options. Eppendorf Bioprocess
Performance Plans help you to maintain the optimal performance of your equipment over years of frequent
use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 199

Service

Service Operation ESSENTIAL CHECK ADVANCED PREMIUM SERVICE

MAINTENANCE
Order Number 0082 140 003 0082 140 004 0082 140 005
External Inspection and Maintenance
Check installation environment ■ ■ ■
Verify utility connections ■ ■ ■
Inspect addition pump heads and motors ■ ■ ■
Inspect motor cables and sensor cables ■ ■ ■
Check vessel for damage ■ ■ ■
Internal Equipment
Check motor and bearing housing for speed and ■ ■ ■
operation1)
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, washers, and ferrules ■ ■
Inspect cooling valve ■ ■ ■
Inspect gas sequencing valves ■ ■ ■
System Checks
Calibrate pH and DO loops with simulator ■ ■ ■
Check gas flow and mixing ■ ■ ■
Verify motor speed1) ■ ■ ■
Verify temperature ■ ■ ■
Perform test run1) ■ ■ ■
Check for latest software revision ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■
Supporting Information
Contract period one year one year one year
Number of preventive services included one one one
Cost of repairs/parts replaced outside scope of not included not included discount on parts, labor,
preventive mainenance visit (where Eppendorf travel time
product warranty has expired)
Note: For customers using BioBLU® Single-Use Bioreactor please select the Essential Check only. Please contact your local Eppendorf representative for other service contract options.
1)
Customers using single-use vessels must provide a vessel for these tests.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
200

Service

BioFlo® 320 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The BioFlo 320, next-generation bioprocess control station, is designed as a universal platform capable of
meeting the ever changing needs of all segments of biotech and pharmaceutical sciences. The BioFlo 320
is suitable for microbial and cell culture, scale up and scale down, and batch, fed batch, and perfusion
processes. It features an extensive range of glass and BioBLU Single-Use Bioreactor options (250 mL – 40 L).
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 201

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation Operational IQ/OQ

CHECK MAINTE- SERVICE Qualification Qualification
NANCE (IQ) (OQ)
Order Number 0082 140 123 0082 140 124 0082 140 125 0082 140 127 0082 140 128 0082 140 129
External Inspection and
Maintenance
Check installation environment ■ ■ ■ ■ ■
Verify utility connections ■ ■ ■ ■ ■
Inspect addition pump heads and ■ ■ ■ ■ ■
motors
Inspect motor cables and sensor ■ ■ ■ ■ ■
cables
Check vessel for damage ■ ■ ■ ■ ■
Internal Equipment
Check motor and bearing housing ■ ■ ■ ■ ■
for speed and operation1)
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ■ ■
ferrules
Check water circulation system ■ ■ ■
Inspect gas sequencing valves ■ ■ ■
System Checks
Calibrate pH and DO loops with ■ ■ ■ ■ ■ ■
simulator
Check gas flow and mixing ■ ■ ■
Verify motor speed1) ■ ■ ■ ■ ■ ■
Verify temperature ■ ■ ■
Perform test run1) ■ ■ ■ ■ ■ ■
Check for latest software revision ■ ■ ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm ■ ■ ■ ■ ■ ■
Eppendorf service
IQ report and signed documentation ■ ■
OQ report and signed ■ ■
documentation
Supporting Information
Contract period one year one year one year n/a n/a n/a
Number of preventive services one one one n/a n/a n/a
included
Cost of repairs/parts replaced not included not included discount on n/a n/a n/a
outside scope of preventive parts, labor,
mainenance visit (where Eppendorf travel time
product warranty has expired)
Note: For customers using BioBLU® Single-Use Bioreactor please select the Essential Check only. Please contact your local Eppendorf representative for other service contract options.
1)
Customers using single-use vessels must provide a vessel for these tests.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
202

Service

BioFlo®/CelliGen 310 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The BioFlo/CelliGen 310 is a benchtop, autoclavable fermentor/bioreactor with advanced controller and
touchscreen interface. The system can operate glass vessels of four sizes ranging from 0.6 L to 10 L, as
well as BioBLU Single-Use Bioreactors covering working volumes of 1.25 L to 40 L.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 203

Service

Service Operation ESSENTIAL CHECK ADVANCED PREMIUM SERVICE

MAINTENANCE
Order Number 0082 140 083 0082 140 084 0082 140 085
External Inspection and Maintenance
Check installation environment ■ ■ ■
Verify utility connections ■ ■ ■
Inspect addition pump heads and motors ■ ■ ■
Inspect motor cables and sensor cables ■ ■ ■
Check vessel for damage ■ ■ ■
Internal Equipment
Check motor and bearing housing for speed and ■ ■ ■
operation1)
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ferrules ■ ■
Check water circulation system ■ ■ ■
Inspect gas sequencing valves ■ ■ ■
System Checks
Calibrate pH and DO loops with simulator ■ ■ ■
Verify motor speed1) ■ ■ ■
Verify temperature ■ ■ ■
Perform test run1) ■ ■ ■
Check for latest software revision ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■
Supporting Information
Contract period one year one year one year
Number of preventive services included one one one
Cost of repairs/parts replaced outside scope of not included not included discount on parts, labor,
preventive mainenance visit (where Eppendorf travel time
product warranty has expired)
Note: For customers using BioBLU® Single-Use Bioreactors please select the Essential Check only. Please contact your local Eppendorf representative for other service contract options.
1)
Customers using single-use vessels must provide a vessel for these tests.

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
204

Service

CelliGen BLU Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime. The CelliGen BLU combines the benefits of single-use technology with
the performance and scalability of a stirredtank design.
The CelliGen BLU features interchangeable pitched-blade, single-use vessels in 5 L, 14 L and 50 L
capacities, as well as a single-use vessel featuring the proprietary Eppendorf packed-bed impeller.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 205

Service

Service Operation ■
 ESSENTIAL CHECK
Order Number 0082 140 253
External Inspection and Maintenance
Check installation environment ■
Verify utility connections ■
Inspect addition pump heads and motors ■
Inspect motor cables and sensor cables ■
System Checks
Calibrate pH and DO loops with simulator ■
Verify gas flow and check mixing ■
Inspect gas sequencing valves ■
Verify motor speed (without vessel) ■
Verify temperature ■
Check for latest software revision ■
Check PI values for inconsistent settings ■
Documentation
Check list provided ■
Dated service sticker to confirm Eppendorf service ■
Supporting Information
Contract period one year
Number of preventive services included one
Cost of repairs/parts replaced outside scope of not included
preventive mainenance visit (where Eppendorf
product warranty has expired)

Specifications subject to change

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
206

Service

BioFlo® 415 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The BioFlo 415 is an economical sterilize-in-place fermentor available in three vessel sizes with working
volumes from 2 L to 15 L. The system's built-in electrical heaters make the BioFlo 415 particularly suitable
for those labs where a steam source is not present.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 207

Service

Service Operation ADVANCED PREMIUM SERVICE Installation Qualification

MAINTENANCE (IQ)
Order Number 0082 140 164 0082 140 165 0082 140 167
External Inspection and Maintenance
Check installation environment ■ ■ ■
Verify utility connections ■ ■ ■
Inspect addition pump heads and motors ■ ■ ■
Inspect motor cables and sensor cables ■ ■ ■
Check vessel for damage and leaks ■ ■ ■
Verify loop PI settings ■ ■ ■
Check valve operation ■ ■ ■
Check hoses ■ ■ ■
Internal Equipment
Check motor and bearing housing for speed and ■ ■
operation
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ferrules ■ ■
Inspect solenoid valves operations ■ ■
Inspect gas sequencing valves ■ ■
Replace rupture disk ■ ■
Test TMFC for operation ■ ■
System Checks
Calibrate pH and DO loops with simulator ■ ■ ■
Verify motor speed ■ ■ ■
Verify temperature ■ ■ ■
Perform test run ■ ■ ■
Check for latest software revision ■ ■ ■
Documentation
Check list provided ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■
IQ report and signed documentation ■
Supporting Information
Contract period one year one year n/a
Number of preventive services included one one n/a
Cost of repairs/parts replaced outside scope of not included discount on parts, labor, n/a
preventive mainenance visit (where Eppendorf travel time
product warranty has expired)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
208

Service

BioFlo®/CelliGen 510 (RPC/AB)

Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The BioFlo/CelliGen 510 is a sterilize-in-place pilot/production-scale fermentor/bioreactor that can be
placed on a laboratory bench or on a mobile cart. The system can be controlled by the Eppendorf RPC
controller or an Allen Bradley Programmable Logic Controller (PLC).
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 209

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation Operational IQ/OQ

CHECK MAINTE- SERVICE Qualification Qualification
NANCE (IQ) (OQ)
Order Number 0082 140 293 0082 140 294 0082 140 295 0082 140 297 0082 140 298 0082 140 299
External Inspection and
Maintenance
Check installation environment ■ ■ ■ ■ ■
Verify utility connections ■ ■ ■ ■ ■
Inspect addition pump heads and ■ ■ ■ ■ ■
motors
Inspect motor cables and sensor ■ ■ ■ ■ ■
cables
Check vessel for damage and leaks ■ ■ ■ ■ ■
Verify loop PI settings ■ ■ ■ ■ ■
Check valve operation ■ ■ ■ ■ ■
Check hoses ■ ■ ■ ■ ■
Internal Equipment
Check motor and bearing housing ■ ■ ■ ■ ■
for speed and operation
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ■ ■ ■
ferrules
Rebuild solenoid, pneumatic, hand- ■ ■ ■
operated, addition, sample, and
harvest valves
Inspect angle seat valves, gas ■ ■ ■
sequencing valves, and circulating
pump
Replace rupture disk ■ ■ ■
Test TMFC for operation ■ ■ ■ ■ ■
System Checks
Calibrate pH and DO loops with ■ ■ ■ ■ ■ ■
simulator
Verify motor speed ■ ■ ■ ■ ■ ■
Verify temperature ■ ■ ■
Perform pressure hold ■ ■ ■ ■ ■ ■
Perform test run ■ ■ ■ ■ ■ ■
Check for latest software revision ■ ■ ■ ■ ■ ■
Verify valve sequence ■ ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm ■ ■ ■ ■ ■ ■
Eppendorf service
IQ report and signed documentation ■ ■
OQ report and signed ■ ■
documentation
Supporting Information
Contract period one year one year one year n/a n/a n/a
Number of preventive services one one one n/a n/a n/a
included
Cost of repairs/parts replaced not included not included discount on n/a n/a n/a
outside scope of preventive parts, labor,
mainenance visit (where Eppendorf travel time
product warranty has expired)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
210

Service

BioFlo® 610 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The BioFlo 610 is a compact, mobile, pilot/production fermentor for technology transfer and small-scale
production. A modular design and wide variety of standard and optional components provide the flexibility
to customize the system to meet your process requirements. The BioFlo 610 is available in two vessel sizes
with working volumes of 16 L to 100 L.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 211

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation Operational IQ/OQ

CHECK MAINTE- SERVICE Qualification Qualification
NANCE (IQ) (OQ)
Order Number 0082 140 333 0082 140 334 0082 140 335 0082 140 337 0082 140 338 0082 140 339
External Inspection and
Maintenance
Check installation environment ■ ■ ■ ■ ■
Verify utility connections ■ ■ ■ ■ ■
Inspect addition pump heads and ■ ■ ■ ■ ■
motors
Inspect motor cables and sensor ■ ■ ■ ■ ■
cables
Check vessel for damage and leaks ■ ■ ■ ■ ■
Verify loop PI settings ■ ■ ■ ■ ■
Check valve operation ■ ■ ■ ■ ■
Check hoses ■ ■ ■ ■ ■
Internal Equipment
Check motor and bearing housing ■ ■ ■ ■ ■
for speed and operation
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ■ ■ ■
ferrules
Rebuild solenoid, pneumatic, hand- ■ ■ ■
operated, addition, sample, and
harvest valves
Inspect angle seat valves, gas ■ ■ ■
sequencing valves, and circulating
pump
Replace rupture disk ■ ■ ■
Test TMFC for operation ■ ■ ■ ■ ■
System Checks
Calibrate pH and DO loops with ■ ■ ■ ■ ■ ■
simulator
Verify motor speed ■ ■ ■ ■ ■ ■
Verify temperature ■ ■ ■
Perform pressure hold ■ ■ ■ ■ ■ ■
Perform test run ■ ■ ■ ■ ■ ■
Check for latest software revision ■ ■ ■ ■ ■ ■
Verify valve sequence ■ ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm ■ ■ ■ ■ ■ ■
Eppendorf service
IQ report and signed documentation ■ ■
OQ report and signed ■ ■
documentation
Supporting Information
Contract period one year one year one year n/a n/a n/a
Number of preventive services one one one n/a n/a n/a
included
Cost of repairs/parts replaced not included not included discount on n/a n/a n/a
outside scope of preventive parts, labor,
mainenance visit (where Eppendorf travel time
product warranty has expired)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
212

Service

BioFlo® 720 Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime. The BioFlo 720 is a compact, mobile, and flexible pilot/ production
bioreactor control system. It supports seamless scalability from 50 L upto 2000 L. Designed to save time,
the BioFlo 720 comes with a variety of new features to automate your workflow and to mitigate risks.
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 213

Service

Service Operation ESSENTIAL ADVANCED PREMIUM IQ/OQ OQ

CHECK MAINTE­ SERVICE
NANCE
Order Number 0082140905 0082140906 0082140907 0082140904 0082140914
External Inspection and Maintenance ■ ■ ■ ■
Check installation environment ■ ■ ■ ■
Verify utility connections ■ ■ ■
Inspect addition pump heads and motors ■ ■
Inspect RTD ■ ■ ■
Inspect temperature control cable ■ ■ ■
Inspect motor cables ■ ■ ■
Inspect sensor cables ■ ■ ■
Verify loop PI settings ■ ■ ■
Check valve operation ■ ■
Check hoses ■ ■ ■
Internal Equipment
Check motor for speed and operation ■ ■ ■ ■ ■
Inspect solenoid valves ■ ■
Test TMFC for operation ■ ■ ■ ■ ■
Inspect TCU ■ ■ ■
Inspect gas sequencing valves ■ ■
System Checks
Calibrate sensors ■ ■ ■ ■ ■
Verify motor speed ■ ■ ■ ■
Perform test run ■ ■
Validation & Metrology
Verify PI values ■ ■ ■ ■ ■
Check for the latest SW revision ■ ■ ■ ■ ■
Verify scale reading operates within specifications ■ ■
Verify shipment vs order ■
Verify TMFC operating within specifications ■ ■
Verify temperature is operating within specifications ■ ■
Verify agitation is operating within specifications ■ ■
Verify external scales and pumps operate within ■ ■
specifications
Test sensor readins in all operation modes ■ ■
Verify Input/Output operarate with in specifications ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm Eppendorf service ■ ■ ■
IQ/OQ report and signed documentation ■ ■
Supporting Information
Contract Period one year one year
Number of preventative services included one one
Cost of repairs/parts replaced outside scope of not included Discounts on
preventive maintenance visit(where Eppendorf parts, labor,
product warranty has expired travel time

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
214

Service

BioFlo® Pro Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The BioFlo Pro fermentor is a modular system designed for quick delivery, dependable operation, and
system flexibility. Systems are available in seven vessel sizes with working volumes from 32 L to 2,400 L.
All BioFlo Pro fermentors utilize industry-standard components including an Allen Bradley® Compact
Programmable Logic Controller (PLC).
Eppendorf Bioprocess Performance Plans help you to maintain the optimal performance of your equipment
over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 215

Service

Service Operation  SSENTIAL

E  DVANCED
A PREMIUM Installation Operational IQ/OQ
CHECK MAINTE- SERVICE Qualification Qualification
NANCE (IQ) (OQ)
Order Number 60L Vessel 0082 140 373 0082 140 374 0082 140 375 0082 140 377 0082 140 378 0082 140 379
Order Number 120 L Vessel 0082 140 463 0082 140 464 0082 140 465 0082 140 377 0082 140 378 0082 140 379
Order Number 240 L Vessel 0082 140 503 0082 140 504 0082 140 505 0082 140 377 0082 140 378 0082 140 379
Order Number 400 L Vessel 0082 140 543 0082 140 544 0082 140 545 0082 140 377 0082 140 378 0082 140 379
Order Number 800 L/1,200 L/2,400 L 0082 140 593 0082 140 594 0082 140 595 0082 140 377 0082 140 378 0082 140 379
Vessels
External Inspection and Maintenance
Check installation environment ■ ■ ■ ■ ■
Verify utility connections ■ ■ ■ ■ ■
Inspect addition pump heads and motors ■ ■ ■ ■ ■
Inspect motor cables and sensor cables ■ ■ ■ ■ ■
Check vessel for damage and leaks ■ ■ ■ ■ ■
Verify loop PI settings ■ ■ ■ ■ ■
Check valve operation ■ ■ ■ ■ ■
Check hoses ■ ■ ■ ■ ■
Internal Equipment
Check motor and bearing housing for ■ ■ ■ ■ ■
speed and operation
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ferrules ■ ■ ■
Rebuild solenoid, pneumatic, hand- ■ ■ ■
operated, addition, sample, and harvest
valves
Inspect angle seat valves, gas sequencing ■ ■ ■
valves, and circulating pump
Replace rupture disk ■ ■ ■
Test TMFC for operation ■ ■ ■ ■ ■
Check PLC battery ■ ■ ■
System Checks
Calibrate pH and DO loops with ■ ■ ■ ■ ■ ■
simulator
Verify motor speed ■ ■ ■ ■ ■ ■
Verify temperature ■ ■ ■
Perform pressure hold ■ ■ ■ ■ ■ ■
Perform test run ■ ■ ■ ■ ■ ■
Check for latest software revision ■ ■ ■ ■ ■ ■
Verify valve sequence ■ ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm ■ ■ ■ ■ ■ ■
Eppendorf service
IQ report and signed documentation ■ ■
OQ report and signed documentation ■ ■
Supporting Information
Contract period one year one year one year n/a n/a n/a
Number of preventive services included one one one n/a n/a n/a
Cost of repairs/parts replaced outside not included not included discount on n/a n/a n/a
scope of preventive mainenance visit parts, labor,
(where Eppendorf product warranty travel time
has expired)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
216

Service

CelliGen Pro Performance Plans

Bioprocess systems include several sophisticated technologies. Peak performance requires smooth
interplay of the subsystems and fully functioning consumable parts. Regular maintenance by qualified
engineers helps to ensure reliable operation, while deferring it can cause unreliable results, expensive
repairs, and prolonged downtime.
The CelliGen Pro bioreactor is a modular system designed for quick delivery, dependable operation, and
system flexibility. Systems are available in four vessel sizes with working volumes from 19 L to 520 L.
All CelliGen Pro bioreactors utilize industry-standard components including an Allen Bradley® Compact
Programmable Logic Controller (PLC). Eppendorf Bioprocess Performance Plans help you to maintain the
optimal performance of your equipment over years of frequent use.

i For more information go to www.eppendorf.link/bioprocess

Technical specifications subject to change.

 217

Service

Service Operation ESSENTIAL ADVANCED PREMIUM Installation Operational IQ/OQ

CHECK MAINTE- SERVICE Qualification Qualification
NANCE (IQ) (OQ)
Order Number 60L Vessel 0082 140 413 0082 140 414 0082 140 415 0082 140 417 0082 140 418 0082 140 419
Order Number 120 L Vessel 0082 140 693 0082 140 694 0082 140 695 0082 140 417 0082 140 418 0082 140 419
Order Number 240 L Vessel 0082 140 743 0082 140 744 0082 140 745 0082 140 417 0082 140 418 0082 140 419
Order Number 520 L Vessel 0082 140 643 0082 140 644 0082 140 645 0082 140 417 0082 140 418 0082 140 419
External Inspection and Maintenance ■ ■ ■ ■ ■
Check installation environment ■ ■ ■ ■ ■
Verify utility connections ■ ■ ■ ■ ■
Inspect addition pump heads and ■ ■ ■ ■ ■
motors
Inspect motor cables and sensor cables ■ ■ ■ ■ ■
Check vessel for damage and leaks ■ ■ ■ ■ ■
Verify loop PI settings ■ ■ ■ ■ ■
Check valve operation ■ ■ ■ ■ ■
Check hoses ■ ■ ■ ■ ■
Internal Equipment
Check motor and bearing housing for ■ ■ ■ ■ ■
speed and operation
Rebuild bearing housing ■ ■
Replace o-rings, gaskets, and ferrules ■ ■ ■
Rebuild solenoid, pneumatic, hand- ■ ■ ■
operated, addition, sample, and
harvest valves
Inspect angle seat valves, gas ■ ■ ■
sequencing valves, and circulating
pump
Replace rupture disk ■ ■ ■
Test TMFC for operation ■ ■ ■ ■ ■
Check PLC battery ■ ■ ■
System Checks
Calibrate pH and DO loops with ■ ■ ■ ■ ■ ■
simulator
Verify motor speed ■ ■ ■ ■ ■ ■
Verify temperature ■ ■ ■
Perform pressure hold ■ ■ ■ ■ ■ ■
Perform test run ■ ■ ■ ■ ■ ■
Check for latest software revision ■ ■ ■ ■ ■ ■
Verify valve sequence ■ ■ ■ ■ ■
Documentation
Check list provided ■ ■ ■
Dated service sticker to confirm ■ ■ ■ ■ ■ ■
Eppendorf service
IQ report and signed documentation ■ ■
OQ report and signed documentation ■ ■
Supporting Information
Contract period one year one year one year n/a n/a n/a
Number of preventive services included one one one n/a n/a n/a
Cost of repairs/parts replaced not included not included discount on n/a n/a n/a
outside scope of preventive parts, labor,
mainenance visit (where Eppendorf travel time
product warranty has expired)

Your local distributor: www.eppendorf.link/distributors

Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany · eppendorf@eppendorf.com · www.eppendorf.com
Application and Technical support

Your local distributor:

www.eppendorf.link/contact

Eppendorf Online Shop

www.eppendorf.link/eshop

Eppendorf General Lab Catalog 2024. More info at www.eppendorf.link/catalog or contact your local dealer.

More information for you:

Our distributors Legal notices, trademarks,

patent and design attributions

Your local contact: Trademarks of third parties may appear

on this catalogue when referring to their
products or services. All trademarks are
the property of their respective owners.
The trademark name representations and
listed owners can be found here:

www.eppendorf.link/ www.eppendorf.link/
distributors/ trademarks

Imprint:
Eppendorf SE E-mail: eppendorf@eppendorf.de Management Board: Chairman of the Supervisory Board:
Barkhausenweg 1 or contact form Dr. Wilhelm Plüster (Spokesperson for the Management Philipp von Loeper
22339 Hamburg Web: www.eppendorf.com Board, Chief Technology Officer), Axel Jaeger (Member
Germany Registration court: of the Management Board, Chief Financial Officer), Turnover tax identification number
Phone: + 49 40 53 801-0 Local Court Hamburg Dr. Dirk Eric Loebermann (Member of the Management pursuant to § 27 a Turnover Tax Law:
Fax: + 49 40 53 801-556 Registration number: HRB 171526 Board, Chief Operating Officer) DE 118649726

Eppendorf® and the Eppendorf Brand Design are registered trademarks of Eppendorf SE, Germany. U.S. Design Patents are listed on www.eppendorf.link/ip.
All rights reserved, including graphics and images. Order No.: AK01 009 820/EN1/012024/CCO_MEP/Copyright© 2024 by Eppendorf SE.